AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27492

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27492.t1 MTR_7g073380 90.816 294 16 2 1 283 1 294 0.0 547
MS.gene27492.t1 MTR_7g073430 87.368 285 32 4 1 283 1 283 0.0 506
MS.gene27492.t1 MTR_0002s1250 40.076 262 132 6 13 264 5 251 5.21e-60 192
MS.gene27492.t1 MTR_8g005750 48.077 208 94 5 14 220 4 198 2.70e-57 185
MS.gene27492.t1 MTR_8g032510 45.540 213 106 5 14 220 7 215 3.19e-56 182
MS.gene27492.t1 MTR_2g075690 39.683 252 118 8 11 231 12 260 6.76e-49 164
MS.gene27492.t1 MTR_2g075700 40.741 243 116 5 17 231 17 259 2.61e-44 152
MS.gene27492.t1 MTR_2g075680 37.838 259 123 6 11 233 12 268 8.76e-42 146
MS.gene27492.t1 MTR_5g067700 38.983 236 109 12 5 238 4 206 6.38e-39 136
MS.gene27492.t1 MTR_3g093830 41.530 183 86 6 21 191 23 196 6.53e-38 136
MS.gene27492.t1 MTR_2g075750 54.455 101 45 1 108 207 13 113 1.01e-34 124
MS.gene27492.t1 MTR_2g075610 53.398 103 47 1 108 209 14 116 1.54e-33 121
MS.gene27492.t1 MTR_7g117200 31.980 197 106 4 12 195 9 190 1.78e-26 106
MS.gene27492.t1 MTR_3g090860 33.503 197 95 5 15 184 7 194 3.17e-26 105
MS.gene27492.t1 MTR_8g027860 51.765 85 33 1 106 182 111 195 4.50e-23 97.4
MS.gene27492.t1 MTR_8g099350 31.863 204 104 7 1 182 1 191 6.26e-21 91.7
MS.gene27492.t1 MTR_5g067680 38.889 108 61 1 75 182 159 261 8.04e-18 82.8
MS.gene27492.t1 MTR_7g028415 51.667 60 26 1 122 181 92 148 2.40e-14 69.7
MS.gene27492.t1 MTR_7g028710 51.667 60 26 1 122 181 92 148 2.40e-14 69.7
MS.gene27492.t1 MTR_5g073620 46.875 64 34 0 121 184 670 733 2.52e-14 73.6
MS.gene27492.t1 MTR_8g096730 35.088 114 68 3 76 189 26 133 3.61e-13 65.9
MS.gene27492.t1 MTR_8g092140 40.964 83 39 3 98 180 142 214 3.73e-13 67.8
MS.gene27492.t1 MTR_7g110720 35.345 116 72 2 66 180 158 271 1.62e-12 67.4
MS.gene27492.t1 MTR_3g095080 41.176 85 37 3 98 181 151 223 2.81e-12 65.5
MS.gene27492.t1 MTR_7g079010 43.077 65 34 1 116 180 134 195 5.04e-12 64.3
MS.gene27492.t1 MTR_3g095080 41.176 85 37 3 98 181 127 199 5.26e-12 64.3
MS.gene27492.t1 MTR_1g086790 46.667 60 29 1 122 181 157 213 5.39e-12 65.5
MS.gene27492.t1 MTR_2g088000 34.579 107 67 2 98 204 325 428 5.54e-12 66.2
MS.gene27492.t1 MTR_5g043880 47.458 59 28 1 122 180 123 178 6.61e-12 64.7
MS.gene27492.t1 MTR_8g007270 33.813 139 62 6 73 205 113 227 7.02e-12 63.9
MS.gene27492.t1 MTR_7g109800 46.667 60 29 1 122 181 144 200 1.50e-11 63.9
MS.gene27492.t1 MTR_3g053330 36.364 99 60 2 106 204 235 330 1.79e-11 64.3
MS.gene27492.t1 MTR_4g094588 47.458 59 28 1 122 180 177 232 4.39e-11 62.8
MS.gene27492.t1 MTR_5g091390 45.763 59 29 1 122 180 156 211 4.79e-11 62.8
MS.gene27492.t1 MTR_8g028565 39.744 78 43 2 106 181 136 211 7.00e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27492.t1 AT3G56400 41.758 182 81 5 14 182 10 179 3.65e-37 134
MS.gene27492.t1 AT2G40750 37.500 200 92 8 14 182 14 211 1.07e-27 109
MS.gene27492.t1 AT1G66550 34.158 202 112 7 14 212 8 191 1.53e-26 104
MS.gene27492.t1 AT2G46400 34.239 184 91 5 15 189 8 170 7.29e-26 103
MS.gene27492.t1 AT5G01900 34.392 189 96 5 10 187 3 174 3.79e-25 101
MS.gene27492.t1 AT1G66550 33.333 201 110 7 14 212 8 186 5.33e-25 100
MS.gene27492.t1 AT5G22570 32.432 185 103 5 14 191 9 178 5.54e-24 98.6
MS.gene27492.t1 AT4G11070 31.959 194 108 4 12 182 8 200 1.08e-22 95.5
MS.gene27492.t1 AT1G66560 33.721 172 98 5 15 185 9 165 6.43e-22 92.4
MS.gene27492.t1 AT1G66600 33.523 176 95 5 14 185 8 165 3.50e-21 90.1
MS.gene27492.t1 AT5G24110 33.333 180 83 4 16 182 17 172 2.08e-20 89.4
MS.gene27492.t1 AT2G40740 30.244 205 115 5 16 195 44 245 8.07e-20 87.4
MS.gene27492.t1 AT2G40740 30.244 205 115 5 16 195 53 254 9.51e-20 87.4
MS.gene27492.t1 AT4G11070 32.948 173 102 3 12 182 8 168 5.35e-19 85.1
MS.gene27492.t1 AT1G80590 36.522 115 65 2 124 233 86 197 6.89e-18 80.9
MS.gene27492.t1 AT4G23810 45.882 85 38 1 106 182 133 217 1.93e-17 81.3
MS.gene27492.t1 AT1G69310 37.097 124 71 4 69 188 89 209 1.93e-14 72.4
MS.gene27492.t1 AT1G69310 37.097 124 71 4 69 188 89 209 1.93e-14 72.4
MS.gene27492.t1 AT1G69310 37.097 124 71 4 69 188 89 209 1.93e-14 72.4
MS.gene27492.t1 AT5G45260 52.632 57 27 0 124 180 1209 1265 3.23e-13 70.1
MS.gene27492.t1 AT5G13080 45.070 71 36 1 122 192 66 133 1.58e-12 64.3
MS.gene27492.t1 AT5G41570 40.964 83 44 2 98 180 75 152 1.84e-12 64.7
MS.gene27492.t1 AT2G37260 45.833 72 35 2 117 188 344 411 2.40e-12 67.0
MS.gene27492.t1 AT2G37260 45.833 72 35 2 117 188 262 329 3.12e-12 66.2
MS.gene27492.t1 AT2G37260 45.833 72 35 2 117 188 264 331 3.20e-12 66.2
MS.gene27492.t1 AT5G49520 41.791 67 36 1 122 188 220 283 5.12e-12 65.9
MS.gene27492.t1 AT2G46130 45.763 59 29 1 122 180 29 84 5.76e-12 61.6
MS.gene27492.t1 AT1G64000 43.077 65 34 1 116 180 107 168 7.78e-12 63.5
MS.gene27492.t1 AT3G01970 38.202 89 44 2 122 210 64 141 1.09e-11 62.0
MS.gene27492.t1 AT4G04450 33.929 112 71 2 94 204 168 277 1.90e-11 64.3
MS.gene27492.t1 AT2G47260 46.667 60 29 1 122 181 173 229 2.59e-11 63.5
MS.gene27492.t1 AT4G04450 33.929 112 71 2 94 204 262 371 2.75e-11 63.9
MS.gene27492.t1 AT4G39410 38.542 96 44 4 93 188 201 281 2.94e-11 63.2
MS.gene27492.t1 AT2G44745 41.667 84 42 3 98 180 122 199 3.73e-11 62.0
MS.gene27492.t1 AT4G18170 36.538 104 54 4 80 180 132 226 3.95e-11 63.2
MS.gene27492.t1 AT1G62300 44.595 74 38 2 108 180 296 367 5.17e-11 63.2
MS.gene27492.t1 AT1G29860 44.444 63 32 1 122 184 135 194 6.75e-11 62.0

Find 57 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGATTGATGAATTGTTAAA+AGG 0.184934 7.2:+31211243 MS.gene27492:CDS
GGTGATATTGATAACATTAA+TGG 0.251913 7.2:+31211309 MS.gene27492:CDS
TCCATTTCTCTCAGTGAAAA+AGG 0.266907 7.2:+31213259 MS.gene27492:CDS
TGCTTTGCAGGAACTATTAC+AGG 0.282092 7.2:+31213027 MS.gene27492:intron
GATTATTGTTCAAGAACAAT+AGG 0.331883 7.2:-31211392 None:intergenic
CAAGAGTACTTCCATTGTTA+AGG 0.344618 7.2:+31211476 MS.gene27492:CDS
CTTTACAAAACAACCTACTA+TGG 0.350539 7.2:+31213119 MS.gene27492:CDS
ACATCATCATACATGATATC+AGG 0.372411 7.2:-31213416 None:intergenic
TTTAAAAGATCTTCAGCAAC+TGG 0.373083 7.2:-31211342 None:intergenic
ATAAATATACCTTGGGAAAT+CGG 0.385972 7.2:-31212634 None:intergenic
CTCTAAAGAATCCTCAGATT+TGG 0.392364 7.2:-31211445 None:intergenic
TCTAATTAATTTCAAGAAAG+TGG 0.395509 7.2:-31213449 None:intergenic
GGTCATCAATGGAGAAAGTA+TGG 0.401550 7.2:+31212591 MS.gene27492:CDS
CTTGATTCTCATGATCAGTT+AGG 0.404590 7.2:+31213383 MS.gene27492:CDS
GGAGAATCCTCTATGATCTC+AGG 0.407384 7.2:-31213170 None:intergenic
CAGTCTTGTTTGGCTGGTGT+TGG 0.412342 7.2:-31213236 None:intergenic
ATTATTGTTCAAGAACAATA+GGG 0.414455 7.2:-31211391 None:intergenic
CCTTCAAATGATTACCTCTC+TGG 0.419974 7.2:+31213314 MS.gene27492:CDS
GAGAAATGGACAGTCTTGTT+TGG 0.445787 7.2:-31213246 None:intergenic
AGTCTTGTTTGGCTGGTGTT+GGG 0.452762 7.2:-31213235 None:intergenic
AGGTAATCATTTGAAGGAGG+AGG 0.457187 7.2:-31213308 None:intergenic
TTCAAGAAAGTGGCCAATTG+AGG 0.469111 7.2:-31213439 None:intergenic
CCAGAGAGGTAATCATTTGA+AGG 0.470477 7.2:-31213314 None:intergenic
TGATTGATGAATTGTTAAAA+GGG 0.474553 7.2:+31211244 MS.gene27492:CDS
CAGAAAAGTTTCACAAACAT+GGG 0.477292 7.2:+31212539 MS.gene27492:intron
AATGGACAGTCTTGTTTGGC+TGG 0.477532 7.2:-31213242 None:intergenic
AGAAATATTCTGAATGAAAG+TGG 0.485810 7.2:+31211288 MS.gene27492:CDS
TCAGAAAAGTTTCACAAACA+TGG 0.494934 7.2:+31212538 MS.gene27492:intron
CTAGCTCATGGTAGTGGTCG+TGG 0.509082 7.2:+31211216 MS.gene27492:CDS
ACAAGTGTGATGGCCATAGT+AGG 0.510474 7.2:-31213132 None:intergenic
AACAATAATCCTACTTCTGA+GGG 0.511593 7.2:+31211405 MS.gene27492:CDS
TCTGATCGTCCCGTGGAAGA+TGG 0.520055 7.2:+31212570 MS.gene27492:CDS
GAAAATCTAGCTCATGGTAG+TGG 0.526481 7.2:+31211210 MS.gene27492:CDS
CCGTGGAAGATGGTCATCAA+TGG 0.528577 7.2:+31212580 MS.gene27492:CDS
AGCTAAGGAACATGGAAGAC+TGG 0.529377 7.2:-31213199 None:intergenic
GTAGAGGCTGTTACAAAAGA+AGG 0.531049 7.2:+31211502 MS.gene27492:CDS
GTTGTCAAAGCTAAGGAACA+TGG 0.540400 7.2:-31213207 None:intergenic
TGTATGATGATGTCCTCAAT+TGG 0.542759 7.2:+31213426 MS.gene27492:CDS
AAATCAGAGCCCTCAGAAGT+AGG 0.544337 7.2:-31211414 None:intergenic
GAACAATAATCCTACTTCTG+AGG 0.546015 7.2:+31211404 MS.gene27492:CDS
GGAAAGAGTTGTCAAAGCTA+AGG 0.561012 7.2:-31213214 None:intergenic
TGCACTCACAAACATGATCA+AGG 0.563442 7.2:+31213050 MS.gene27492:CDS
GAGAATCCTCTATGATCTCA+GGG 0.568526 7.2:-31213169 None:intergenic
CCATTGATGACCATCTTCCA+CGG 0.575191 7.2:-31212580 None:intergenic
GCAAGATTCTACAAGTGTGA+TGG 0.576779 7.2:-31213142 None:intergenic
GCCTCTACGTTCCTTAACAA+TGG 0.594370 7.2:-31211487 None:intergenic
AGTTTCACAAACATGGGAGA+AGG 0.598123 7.2:+31212545 MS.gene27492:CDS
ACAAGTGCAACAAATTCAAG+AGG 0.601952 7.2:+31213088 MS.gene27492:CDS
AACATGGAAGACTGGTCACA+AGG 0.611016 7.2:-31213191 None:intergenic
CATTGATGACCATCTTCCAC+GGG 0.612085 7.2:-31212579 None:intergenic
TTTGCAACTCACCAAATCTG+AGG 0.616026 7.2:+31211434 MS.gene27492:CDS
TCTTGCACACCGATTTCCCA+AGG 0.624999 7.2:+31212625 MS.gene27492:CDS
GCAAAGCCCTGAGATCATAG+AGG 0.632964 7.2:+31213163 MS.gene27492:CDS
GAGAGGTAATCATTTGAAGG+AGG 0.645608 7.2:-31213311 None:intergenic
TCCATTGTTAAGGAACGTAG+AGG 0.656442 7.2:+31211486 MS.gene27492:CDS
GAAGGAGTCTGATCGTCCCG+TGG 0.657169 7.2:+31212563 MS.gene27492:CDS
CATCAAAAGTGAGTCCAGAG+AGG 0.707648 7.2:-31213328 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAAAAAATGAGA+GGG - chr7.2:31212351-31212370 None:intergenic 10.0%
!! AATGTATTTGGTTAAAAAAT+TGG + chr7.2:31211968-31211987 MS.gene27492:intron 15.0%
!! CAAAATAAATAAAAAATGAG+AGG - chr7.2:31212352-31212371 None:intergenic 15.0%
!! TTAATTTCTTTACATCTTAA+AGG + chr7.2:31212843-31212862 MS.gene27492:intron 15.0%
!! AGTAAAAAAAGTTGAATATG+TGG - chr7.2:31211562-31211581 None:intergenic 20.0%
!! ATATAGAATTATAGATGTGT+TGG - chr7.2:31211657-31211676 None:intergenic 20.0%
!! ATATTACTCATATGTTGTAA+AGG - chr7.2:31212069-31212088 None:intergenic 20.0%
!! ATGATGAACAATAATTGATA+GGG - chr7.2:31212278-31212297 None:intergenic 20.0%
!! ATTATTGTTCAAGAACAATA+GGG - chr7.2:31211394-31211413 None:intergenic 20.0%
!! ATTGTTCTTATAAATAGGAT+CGG - chr7.2:31211931-31211950 None:intergenic 20.0%
!! CCAAAATTGTTCTTATAAAT+AGG - chr7.2:31211936-31211955 None:intergenic 20.0%
!! CCTATTTATAAGAGAAATTT+GGG - chr7.2:31212018-31212037 None:intergenic 20.0%
!! GACAATCTTAAAAGTAAAAT+GGG - chr7.2:31212681-31212700 None:intergenic 20.0%
!! TACAACATATGAGTAATATT+GGG + chr7.2:31212070-31212089 MS.gene27492:intron 20.0%
!! TATAGAATTATAGATGTGTT+GGG - chr7.2:31211656-31211675 None:intergenic 20.0%
!! TATTAAGAAAGAAGAATTAG+AGG - chr7.2:31212969-31212988 None:intergenic 20.0%
!! TCAACAAAAGTTAATGTATT+TGG + chr7.2:31211956-31211975 MS.gene27492:intron 20.0%
!! TCCTATTTATAAGAGAAATT+TGG - chr7.2:31212019-31212038 None:intergenic 20.0%
!! TGAAAAAGAATTGCATAATT+GGG - chr7.2:31212521-31212540 None:intergenic 20.0%
!! TGACAATCTTAAAAGTAAAA+TGG - chr7.2:31212682-31212701 None:intergenic 20.0%
!! TGATTGATGAATTGTTAAAA+GGG + chr7.2:31211244-31211263 MS.gene27492:CDS 20.0%
!! TTACAACATATGAGTAATAT+TGG + chr7.2:31212069-31212088 MS.gene27492:intron 20.0%
!! TTCAAATAGTGAAATTTGAT+TGG - chr7.2:31212711-31212730 None:intergenic 20.0%
!!! AATTTTAGATGTATGAAAAG+TGG - chr7.2:31211680-31211699 None:intergenic 20.0%
!!! CCTATTTATAAGAACAATTT+TGG + chr7.2:31211933-31211952 MS.gene27492:intron 20.0%
!!! TAAAATGTTAAATGAGTTGA+AGG + chr7.2:31212890-31212909 MS.gene27492:intron 20.0%
!!! TTATTTGAACTTTTAAGATG+AGG + chr7.2:31211846-31211865 MS.gene27492:intron 20.0%
!!! TTGTTGTGTTTTTTTTTTTG+TGG + chr7.2:31212369-31212388 MS.gene27492:intron 20.0%
!!! TTTTCCTTTTTTTTCTTCTT+CGG + chr7.2:31211750-31211769 MS.gene27492:intron 20.0%
!!! TTTTGATGAGAGAAAATTAT+TGG - chr7.2:31212159-31212178 None:intergenic 20.0%
! AAACTCGTATAAATATACCT+TGG - chr7.2:31212645-31212664 None:intergenic 25.0%
! AACTCGTATAAATATACCTT+GGG - chr7.2:31212644-31212663 None:intergenic 25.0%
! ACAACATATGAGTAATATTG+GGG + chr7.2:31212071-31212090 MS.gene27492:intron 25.0%
! AGAAATATTCTGAATGAAAG+TGG + chr7.2:31211288-31211307 MS.gene27492:CDS 25.0%
! ATAAATATACCTTGGGAAAT+CGG - chr7.2:31212637-31212656 None:intergenic 25.0%
! ATAGTGAAATTTGATTGGAT+TGG - chr7.2:31212706-31212725 None:intergenic 25.0%
! ATTTCTCTTATAAATAGGAC+CGG + chr7.2:31212020-31212039 MS.gene27492:intron 25.0%
! CCCAAATTTCTCTTATAAAT+AGG + chr7.2:31212015-31212034 MS.gene27492:intron 25.0%
! CTGAAAAAGAATTGCATAAT+TGG - chr7.2:31212522-31212541 None:intergenic 25.0%
! GAAAAACCATAGGTTAAAAT+AGG - chr7.2:31211610-31211629 None:intergenic 25.0%
! GATGATGAACAATAATTGAT+AGG - chr7.2:31212279-31212298 None:intergenic 25.0%
! GATTATTGTTCAAGAACAAT+AGG - chr7.2:31211395-31211414 None:intergenic 25.0%
! GTGATTGATGAATTGTTAAA+AGG + chr7.2:31211243-31211262 MS.gene27492:CDS 25.0%
! TGAATATTTATATGCTTTGC+AGG + chr7.2:31213015-31213034 MS.gene27492:intron 25.0%
!! AAAAATGAAAATCTAGCTCA+TGG + chr7.2:31211204-31211223 MS.gene27492:CDS 25.0%
!! ATGAGAGAAAATTATTGGTT+AGG - chr7.2:31212154-31212173 None:intergenic 25.0%
!! GGTGATATTGATAACATTAA+TGG + chr7.2:31211309-31211328 MS.gene27492:CDS 25.0%
!! TTTCATAGTCTTACATTGAT+TGG + chr7.2:31212461-31212480 MS.gene27492:intron 25.0%
!! TTTTTCATACACACTATTCA+TGG + chr7.2:31211624-31211643 MS.gene27492:intron 25.0%
!!! GATTGGTTTTGAGTTTTTTT+TGG + chr7.2:31212478-31212497 MS.gene27492:intron 25.0%
AACAATAATCCTACTTCTGA+GGG + chr7.2:31211405-31211424 MS.gene27492:CDS 30.0%
AATTGATAGGGTCCAATATT+AGG - chr7.2:31212266-31212285 None:intergenic 30.0%
ACATCATCATACATGATATC+AGG - chr7.2:31213419-31213438 None:intergenic 30.0%
ATTGATAGGGTCCAATATTA+GGG - chr7.2:31212265-31212284 None:intergenic 30.0%
CAGAAAAGTTTCACAAACAT+GGG + chr7.2:31212539-31212558 MS.gene27492:intron 30.0%
CTTTACAAAACAACCTACTA+TGG + chr7.2:31213119-31213138 MS.gene27492:CDS 30.0%
CTTTCCGAAGAAGAAAAAAA+AGG - chr7.2:31211757-31211776 None:intergenic 30.0%
TAGTGTGTATGAAAAACCAT+AGG - chr7.2:31211620-31211639 None:intergenic 30.0%
TCAGAAAAGTTTCACAAACA+TGG + chr7.2:31212538-31212557 MS.gene27492:intron 30.0%
TTCTTATAAATAGGATCGGT+GGG - chr7.2:31211927-31211946 None:intergenic 30.0%
TTTAAAAGATCTTCAGCAAC+TGG - chr7.2:31211345-31211364 None:intergenic 30.0%
! AGTTGCTGAAGATCTTTTAA+AGG + chr7.2:31211344-31211363 MS.gene27492:CDS 30.0%
! GTTGTTTCCTCTAGTTATTA+TGG + chr7.2:31212407-31212426 MS.gene27492:intron 30.0%
! TCCTCTAGTTATTATGGTTT+TGG + chr7.2:31212413-31212432 MS.gene27492:intron 30.0%
!! GTGACTTTAGTGTATTTTAG+AGG - chr7.2:31212217-31212236 None:intergenic 30.0%
!! TAGTAGGTTGTTTTGTAAAG+AGG - chr7.2:31213119-31213138 None:intergenic 30.0%
!!! AGTTGACCTATTTTAACCTA+TGG + chr7.2:31211601-31211620 MS.gene27492:intron 30.0%
ACAAGTGCAACAAATTCAAG+AGG + chr7.2:31213088-31213107 MS.gene27492:CDS 35.0%
ACATATGAGTAATATTGGGG+TGG + chr7.2:31212074-31212093 MS.gene27492:intron 35.0%
ATATGAGTAATATTGGGGTG+GGG + chr7.2:31212076-31212095 MS.gene27492:intron 35.0%
ATTTCTCTCAGTGAAAAAGG+AGG + chr7.2:31213262-31213281 MS.gene27492:CDS 35.0%
CATATGAGTAATATTGGGGT+GGG + chr7.2:31212075-31212094 MS.gene27492:intron 35.0%
CTTGATTCTCATGATCAGTT+AGG + chr7.2:31213383-31213402 MS.gene27492:CDS 35.0%
GAACAATAATCCTACTTCTG+AGG + chr7.2:31211404-31211423 MS.gene27492:CDS 35.0%
GCCAAAACCATAATAACTAG+AGG - chr7.2:31212417-31212436 None:intergenic 35.0%
GTTCTTATAAATAGGATCGG+TGG - chr7.2:31211928-31211947 None:intergenic 35.0%
TATCCACTACATGTGATGAA+AGG + chr7.2:31212309-31212328 MS.gene27492:intron 35.0%
TCCATTTCTCTCAGTGAAAA+AGG + chr7.2:31213259-31213278 MS.gene27492:CDS 35.0%
! CTCTAAAGAATCCTCAGATT+TGG - chr7.2:31211448-31211467 None:intergenic 35.0%
! TGTATGATGATGTCCTCAAT+TGG + chr7.2:31213426-31213445 MS.gene27492:CDS 35.0%
!! CAAGAGTACTTCCATTGTTA+AGG + chr7.2:31211476-31211495 MS.gene27492:CDS 35.0%
!! CACTTTTGATGATCCAGAAA+AGG + chr7.2:31213340-31213359 MS.gene27492:CDS 35.0%
!! TAGGTTGTTTTGTAAAGAGG+TGG - chr7.2:31213116-31213135 None:intergenic 35.0%
!! TCCTTTTTCACTGAGAGAAA+TGG - chr7.2:31213263-31213282 None:intergenic 35.0%
AGGTAATCATTTGAAGGAGG+AGG - chr7.2:31213311-31213330 None:intergenic 40.0%
AGTCCTTTCATCACATGTAG+TGG - chr7.2:31212315-31212334 None:intergenic 40.0%
AGTGAAAAAGGAGGATTGCA+AGG + chr7.2:31213271-31213290 MS.gene27492:CDS 40.0%
AGTTTCACAAACATGGGAGA+AGG + chr7.2:31212545-31212564 MS.gene27492:CDS 40.0%
ATAGGGTCCAATATTAGGGT+GGG - chr7.2:31212261-31212280 None:intergenic 40.0%
CCAATAATAAGTACTCCCTC+CGG - chr7.2:31212042-31212061 None:intergenic 40.0%
CCAGAGAGGTAATCATTTGA+AGG - chr7.2:31213317-31213336 None:intergenic 40.0%
CCTTCAAATGATTACCTCTC+TGG + chr7.2:31213314-31213333 MS.gene27492:CDS 40.0%
CTCTTATAAATAGGACCGGA+GGG + chr7.2:31212024-31212043 MS.gene27492:intron 40.0%
GAGAATCCTCTATGATCTCA+GGG - chr7.2:31213172-31213191 None:intergenic 40.0%
GAGAGGTAATCATTTGAAGG+AGG - chr7.2:31213314-31213333 None:intergenic 40.0%
GCAAGATTCTACAAGTGTGA+TGG - chr7.2:31213145-31213164 None:intergenic 40.0%
GGAAAGAGTTGTCAAAGCTA+AGG - chr7.2:31213217-31213236 None:intergenic 40.0%
GGTCATCAATGGAGAAAGTA+TGG + chr7.2:31212591-31212610 MS.gene27492:CDS 40.0%
GTAGAGGCTGTTACAAAAGA+AGG + chr7.2:31211502-31211521 MS.gene27492:CDS 40.0%
GTTGTCAAAGCTAAGGAACA+TGG - chr7.2:31213210-31213229 None:intergenic 40.0%
TCCATTGTTAAGGAACGTAG+AGG + chr7.2:31211486-31211505 MS.gene27492:CDS 40.0%
TCTCTTATAAATAGGACCGG+AGG + chr7.2:31212023-31212042 MS.gene27492:intron 40.0%
TGCACTCACAAACATGATCA+AGG + chr7.2:31213050-31213069 MS.gene27492:CDS 40.0%
TGCTTTGCAGGAACTATTAC+AGG + chr7.2:31213027-31213046 MS.gene27492:intron 40.0%
TTTGCAACTCACCAAATCTG+AGG + chr7.2:31211434-31211453 MS.gene27492:CDS 40.0%
! GAAAATCTAGCTCATGGTAG+TGG + chr7.2:31211210-31211229 MS.gene27492:CDS 40.0%
! GATAGTGTGACATCCTTTTC+TGG - chr7.2:31213356-31213375 None:intergenic 40.0%
! GGAGAAAGTATGGCCAAAAA+AGG + chr7.2:31212601-31212620 MS.gene27492:CDS 40.0%
! GGCTTTAGTGACTTTGTGTT+TGG - chr7.2:31212239-31212258 None:intergenic 40.0%
!! ATAGAGACAAAAGCACCGAA+AGG - chr7.2:31212125-31212144 None:intergenic 40.0%
!! GAGAAATGGACAGTCTTGTT+TGG - chr7.2:31213249-31213268 None:intergenic 40.0%
AAATCAGAGCCCTCAGAAGT+AGG - chr7.2:31211417-31211436 None:intergenic 45.0%
ACAAGTGTGATGGCCATAGT+AGG - chr7.2:31213135-31213154 None:intergenic 45.0%
AGCTAAGGAACATGGAAGAC+TGG - chr7.2:31213202-31213221 None:intergenic 45.0%
CATCAAAAGTGAGTCCAGAG+AGG - chr7.2:31213331-31213350 None:intergenic 45.0%
CTAAAGCCCCACCCTAATAT+TGG + chr7.2:31212251-31212270 MS.gene27492:intron 45.0%
GATAGGGTCCAATATTAGGG+TGG - chr7.2:31212262-31212281 None:intergenic 45.0%
GCCTCTACGTTCCTTAACAA+TGG - chr7.2:31211490-31211509 None:intergenic 45.0%
GGAGAATCCTCTATGATCTC+AGG - chr7.2:31213173-31213192 None:intergenic 45.0%
TAGGGTCCAATATTAGGGTG+GGG - chr7.2:31212260-31212279 None:intergenic 45.0%
! AACATGGAAGACTGGTCACA+AGG - chr7.2:31213194-31213213 None:intergenic 45.0%
! CATTGATGACCATCTTCCAC+GGG - chr7.2:31212582-31212601 None:intergenic 45.0%
! CCATTGATGACCATCTTCCA+CGG - chr7.2:31212583-31212602 None:intergenic 45.0%
!! AATGGACAGTCTTGTTTGGC+TGG - chr7.2:31213245-31213264 None:intergenic 45.0%
!! AGTCTTGTTTGGCTGGTGTT+GGG - chr7.2:31213238-31213257 None:intergenic 45.0%
!! CCGGAGGGAGTACTTATTAT+TGG + chr7.2:31212039-31212058 MS.gene27492:intron 45.0%
!!! CGGTGTGCAAGATCCTTTTT+TGG - chr7.2:31212617-31212636 None:intergenic 45.0%
CCGTGGAAGATGGTCATCAA+TGG + chr7.2:31212580-31212599 MS.gene27492:CDS 50.0%
GCAAAGCCCTGAGATCATAG+AGG + chr7.2:31213163-31213182 MS.gene27492:CDS 50.0%
!! ACTTTGGCTTCGGTGCCTTT+CGG + chr7.2:31212107-31212126 MS.gene27492:intron 50.0%
!! CAGTCTTGTTTGGCTGGTGT+TGG - chr7.2:31213239-31213258 None:intergenic 50.0%
!! TCTTGCACACCGATTTCCCA+AGG + chr7.2:31212625-31212644 MS.gene27492:CDS 50.0%
TCTGATCGTCCCGTGGAAGA+TGG + chr7.2:31212570-31212589 MS.gene27492:CDS 55.0%
! GGCTTCCGTGACTTTGGCTT+CGG + chr7.2:31212097-31212116 MS.gene27492:intron 55.0%
!! AGGCACCGAAGCCAAAGTCA+CGG - chr7.2:31212105-31212124 None:intergenic 55.0%
!! CTAGCTCATGGTAGTGGTCG+TGG + chr7.2:31211216-31211235 MS.gene27492:CDS 55.0%
GAAGGAGTCTGATCGTCCCG+TGG + chr7.2:31212563-31212582 MS.gene27492:CDS 60.0%
GGGTGGGGCTTCCGTGACTT+TGG + chr7.2:31212091-31212110 MS.gene27492:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 31211201 31213460 31211201 ID=MS.gene27492
chr7.2 mRNA 31211201 31213460 31211201 ID=MS.gene27492.t1;Parent=MS.gene27492
chr7.2 exon 31211201 31211523 31211201 ID=MS.gene27492.t1.exon1;Parent=MS.gene27492.t1
chr7.2 CDS 31211201 31211523 31211201 ID=cds.MS.gene27492.t1;Parent=MS.gene27492.t1
chr7.2 exon 31212542 31212646 31212542 ID=MS.gene27492.t1.exon2;Parent=MS.gene27492.t1
chr7.2 CDS 31212542 31212646 31212542 ID=cds.MS.gene27492.t1;Parent=MS.gene27492.t1
chr7.2 exon 31213037 31213460 31213037 ID=MS.gene27492.t1.exon3;Parent=MS.gene27492.t1
chr7.2 CDS 31213037 31213460 31213037 ID=cds.MS.gene27492.t1;Parent=MS.gene27492.t1
Gene Sequence

>MS.gene27492

ATGAAAAATGAAAATCTAGCTCATGGTAGTGGTCGTGGAAAAGTGATTGATGAATTGTTAAAAGGGCGTGAACTTGCACACAAACTTAGAAATATTCTGAATGAAAGTGGTGATATTGATAACATTAATGGATCAACAACACCAGTTGCTGAAGATCTTTTAAAGGAAGTGCTTACAACTTTTACTAATTCCCTATTGTTCTTGAACAATAATCCTACTTCTGAGGGCTCTGATTTGCAACTCACCAAATCTGAGGATTCTTTAGAGAGTAATTGCAAGAGTACTTCCATTGTTAAGGAACGTAGAGGCTGTTACAAAAGAAGGTATTTTTTTTTTTTACTAATTACTAATTTTGCTTCCACATATTCAACTTTTTTTACTATATGAGATCTATATAAATAGTTGACCTATTTTAACCTATGGTTTTTCATACACACTATTCATGGATCTCTCCCAACACATCTATAATTCTATATCCACTTTTCATACATCTAAAATTTTTATATGATTTTTTTTATTCATACATGCATAGTTTTATATATATAGTGTTTTTCCTTTTTTTTCTTCTTCGGAAAGTATTTTATTTTAGCTGATTTTTTAATTTTATTTTTCTTCTCTTATTTCTTGACTTATATAATTTCAATTTTATTTGAACTTTTAAGATGAGGTAATGTATAGTTTGTTCTTTAGTATGATTAGCGATTATAATTGCTAATTAATACTCCCACCGATCCTATTTATAAGAACAATTTTGGTCAACAAAAGTTAATGTATTTGGTTAAAAAATTGGACAAAATACATTCACTTTTGTTGACCCAAATTTCTCTTATAAATAGGACCGGAGGGAGTACTTATTATTGGTTGTCCTTTACAACATATGAGTAATATTGGGGTGGGGCTTCCGTGACTTTGGCTTCGGTGCCTTTCGGTGCTTTTGTCTCTATCTCTCTCCTAACCAATAATTTTCTCTCATCAAAATAATTTATTTTAATCATTCAATCATCACTCTCTTTCCTCTAAAATACACTAAAGTCACCAAACACAAAGTCACTAAAGCCCCACCCTAATATTGGACCCTATCAATTATTGTTCATCATCATTGTCATCATATCCACTACATGTGATGAAAGGACTATGTTGCAATTATCCCTCTCATTTTTTATTTATTTTGTTGTGTTTTTTTTTTTGTGGTGCTTTATTTATTTTGTTGTTTCCTCTAGTTATTATGGTTTTGGCTGACACAGTAAAATTATTTGATTGTTTCATAGTCTTACATTGATTGGTTTTGAGTTTTTTTTGGTAAACTTATAAAACTTACCCAATTATGCAATTCTTTTTCAGAAAAGTTTCACAAACATGGGAGAAGGAGTCTGATCGTCCCGTGGAAGATGGTCATCAATGGAGAAAGTATGGCCAAAAAAGGATCTTGCACACCGATTTCCCAAGGTATATTTATACGAGTTTAATTAAGTACAAACCCATTTTACTTTTAAGATTGTCATCCAATCCAATCAAATTTCACTATTTGAACACATTATCATGTTAACTGATTTTTAAATTAATTAATTTAATTACTACGATGTCGAGATTTGATTTAATAATTAATTAGTAAAATGATTTATAATATCAATGTATATATAATTTAATTTCTTTACATCTTAAAGGATTGAGTCGATCAACTGATTTGAATAAAATGTTAAATGAGTTGAAGGAGTATCTTAGTTCAAATTTTAAAACTTATATTTAGTTTTATACAAAAAAAATTCCTCTAATTCTTCTTTCTTAATATCTCTTCTATATGCTAAGAAGCTAAATGAATATTTATATGCTTTGCAGGAACTATTACAGGTGCACTCACAAACATGATCAAGGTTGCAAAGCGACAAAACAAGTGCAACAAATTCAAGAGGAACCACCTCTTTACAAAACAACCTACTATGGCCATCACACTTGTAGAATCTTGCAAAGCCCTGAGATCATAGAGGATTCTCCTTGTGACCAGTCTTCCATGTTCCTTAGCTTTGACAACTCTTTCCCAACACCAGCCAAACAAGACTGTCCATTTCTCTCAGTGAAAAAGGAGGATTGCAAGGAAGAGATTGTTCATCCTCCTCCTTCAAATGATTACCTCTCTGGACTCACTTTTGATGATCCAGAAAAGGATGTCACACTATCATCAACACTTGATTCTCATGATCAGTTAGGTGTCAACATTCCTGATATCATGTATGATGATGTCCTCAATTGGCCACTTTCTTGA
Protein sequence

>MS.gene27492.t1

MKNENLAHGSGRGKVIDELLKGRELAHKLRNILNESGDIDNINGSTTPVAEDLLKEVLTTFTNSLLFLNNNPTSEGSDLQLTKSEDSLESNCKSTSIVKERRGCYKRRKVSQTWEKESDRPVEDGHQWRKYGQKRILHTDFPRNYYRCTHKHDQGCKATKQVQQIQEEPPLYKTTYYGHHTCRILQSPEIIEDSPCDQSSMFLSFDNSFPTPAKQDCPFLSVKKEDCKEEIVHPPPSNDYLSGLTFDDPEKDVTLSSTLDSHDQLGVNIPDIMYDDVLNWPLS