AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27553

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27553.t1 MTR_7g074850 94.545 330 17 1 1 330 1 329 0.0 647
MS.gene27553.t1 MTR_7g074820 89.394 330 34 1 1 330 1 329 0.0 607
MS.gene27553.t1 MTR_7g074730 85.455 330 46 2 1 330 1 328 0.0 583
MS.gene27553.t1 MTR_7g074880 75.455 330 77 2 1 330 1 326 0.0 526
MS.gene27553.t1 MTR_7g074710 66.061 330 108 2 1 330 1 326 9.54e-165 462
MS.gene27553.t1 MTR_7g074870 72.222 288 77 1 43 330 4 288 7.81e-156 438
MS.gene27553.t1 MTR_4g023730 51.057 331 159 2 1 330 1 329 7.90e-111 325
MS.gene27553.t1 MTR_1g022440 47.059 323 166 4 8 330 8 325 1.38e-99 297
MS.gene27553.t1 MTR_1g022445 46.749 323 167 4 8 330 8 325 4.54e-97 290
MS.gene27553.t1 MTR_4g092080 41.071 336 184 6 1 328 7 336 8.71e-80 246
MS.gene27553.t1 MTR_4g081440 37.243 341 190 9 6 330 22 354 8.32e-72 227
MS.gene27553.t1 MTR_4g081440 37.243 341 189 10 6 330 18 349 2.20e-70 223
MS.gene27553.t1 MTR_2g101330 37.615 327 190 9 7 330 4 319 1.32e-64 207
MS.gene27553.t1 MTR_3g005210 35.061 328 195 8 8 330 6 320 1.51e-58 191
MS.gene27553.t1 MTR_8g062110 36.446 332 192 9 1 327 2 319 1.72e-57 189
MS.gene27553.t1 MTR_8g062110 36.145 332 193 8 1 327 11 328 2.61e-57 189
MS.gene27553.t1 MTR_2g013230 34.971 346 203 8 1 330 1 340 2.63e-57 189
MS.gene27553.t1 MTR_8g062440 36.119 335 196 8 1 330 1 322 9.35e-57 187
MS.gene27553.t1 MTR_4g077130 35.821 335 196 12 1 329 1 322 1.12e-56 187
MS.gene27553.t1 MTR_5g056600 35.583 326 195 9 9 330 5 319 5.26e-56 185
MS.gene27553.t1 MTR_8g062110 37.809 283 163 5 1 280 2 274 1.49e-54 180
MS.gene27553.t1 MTR_8g062440 35.821 335 196 9 1 330 1 321 1.50e-54 181
MS.gene27553.t1 MTR_8g062110 37.809 283 163 5 1 280 2 274 1.63e-54 179
MS.gene27553.t1 MTR_3g005170 34.251 327 197 9 8 329 10 323 1.31e-53 179
MS.gene27553.t1 MTR_3g031650 34.940 332 190 11 8 330 5 319 1.35e-52 176
MS.gene27553.t1 MTR_3g005170 36.462 277 164 6 8 281 10 277 6.75e-52 173
MS.gene27553.t1 MTR_2g013250 33.724 341 206 8 3 328 2 337 6.86e-52 175
MS.gene27553.t1 MTR_8g062440 38.163 283 162 6 1 280 1 273 1.99e-51 173
MS.gene27553.t1 MTR_4g077100 36.140 285 169 7 1 281 3 278 7.23e-51 170
MS.gene27553.t1 MTR_4g077100 36.491 285 168 8 1 281 3 278 2.25e-50 170
MS.gene27553.t1 MTR_5g072620 35.542 332 188 11 8 330 5 319 1.81e-48 165
MS.gene27553.t1 MTR_2g013260 35.174 344 201 9 1 328 1 338 3.02e-48 166
MS.gene27553.t1 MTR_4g009040 33.945 327 198 9 7 330 12 323 3.86e-47 162
MS.gene27553.t1 MTR_3g031650 35.842 279 161 8 8 280 5 271 1.19e-46 159
MS.gene27553.t1 MTR_4g009040 33.233 331 199 9 7 330 12 327 1.39e-45 158
MS.gene27553.t1 MTR_7g117190 33.333 345 207 11 3 330 2 340 5.43e-45 157
MS.gene27553.t1 MTR_2g104960 34.557 327 193 13 8 330 20 329 2.44e-44 155
MS.gene27553.t1 MTR_4g006940 33.333 327 197 11 8 330 17 326 4.00e-44 154
MS.gene27553.t1 MTR_2g028620 33.333 345 192 13 2 330 4 326 3.25e-43 152
MS.gene27553.t1 MTR_2g013260 34.915 295 175 6 1 280 1 293 5.17e-42 147
MS.gene27553.t1 MTR_4g009690 32.722 327 201 9 7 330 12 322 7.27e-41 145
MS.gene27553.t1 MTR_4g009690 32.515 326 204 8 7 330 12 323 1.82e-40 144
MS.gene27553.t1 MTR_4g006940 35.018 277 164 8 8 282 17 279 1.83e-40 143
MS.gene27553.t1 MTR_4g006940 35.018 277 164 8 8 282 17 279 1.83e-40 143
MS.gene27553.t1 MTR_2g028620 35.461 282 162 9 2 275 4 273 2.48e-40 144
MS.gene27553.t1 MTR_7g117190 34.014 294 176 9 3 280 2 293 1.67e-38 139
MS.gene27553.t1 MTR_3g005170 30.581 327 178 10 8 329 10 292 6.35e-38 137
MS.gene27553.t1 MTR_2g013230 31.707 246 148 7 101 330 9 250 1.59e-28 111
MS.gene27553.t1 MTR_8g062110 32.159 227 137 7 106 327 19 233 2.36e-26 105
MS.gene27553.t1 MTR_5g029990 29.137 278 162 11 8 276 12 263 5.15e-24 100
MS.gene27553.t1 MTR_0164s0070 38.760 129 76 2 7 134 4 130 8.87e-24 97.4
MS.gene27553.t1 MTR_8g062110 33.146 178 108 3 106 280 19 188 1.71e-23 95.9
MS.gene27553.t1 MTR_6g406250 28.352 261 167 9 5 257 52 300 1.43e-20 91.3
MS.gene27553.t1 MTR_7g117190 31.466 232 138 9 116 330 3 230 1.58e-20 89.0
MS.gene27553.t1 MTR_2g013260 31.765 255 150 9 90 328 21 267 2.38e-19 86.7
MS.gene27553.t1 MTR_7g074865 85.366 41 6 0 1 41 1 41 3.36e-19 80.9
MS.gene27553.t1 MTR_6g406250 27.232 224 146 7 2 217 47 261 1.42e-17 81.6
MS.gene27553.t1 MTR_3g101500 27.574 272 164 8 7 272 3 247 1.76e-14 73.6
MS.gene27553.t1 MTR_3g101500 27.574 272 164 8 7 272 3 247 1.83e-14 73.2
MS.gene27553.t1 MTR_0685s0030 31.624 117 76 1 165 281 3 115 1.66e-13 66.6
MS.gene27553.t1 MTR_3g101500 30.208 192 113 5 7 198 3 173 2.58e-13 68.6
MS.gene27553.t1 MTR_0685s0030 31.624 117 76 1 165 281 3 115 4.65e-13 66.6
MS.gene27553.t1 MTR_3g011130 33.913 115 69 3 106 216 35 146 7.74e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27553.t1 AT2G45400 50.751 333 156 4 3 330 35 364 1.51e-107 318
MS.gene27553.t1 AT5G42800 48.036 331 165 5 1 330 1 325 1.52e-99 298
MS.gene27553.t1 AT2G45400 51.034 290 135 3 3 287 35 322 6.03e-95 285
MS.gene27553.t1 AT2G45400 50.000 294 139 4 42 330 17 307 2.58e-88 267
MS.gene27553.t1 AT4G35420 41.667 336 175 9 1 330 1 321 1.10e-81 251
MS.gene27553.t1 AT2G45400 53.636 220 97 2 3 217 35 254 3.15e-73 227
MS.gene27553.t1 AT1G61720 39.275 331 189 7 7 330 12 337 1.03e-72 228
MS.gene27553.t1 AT1G68540 39.571 326 182 10 10 330 6 321 2.72e-61 198
MS.gene27553.t1 AT5G58490 37.176 347 176 12 1 330 2 323 2.28e-57 188
MS.gene27553.t1 AT2G02400 36.795 337 181 12 5 330 3 318 3.60e-57 188
MS.gene27553.t1 AT5G19440 37.003 327 188 9 8 329 10 323 1.44e-56 186
MS.gene27553.t1 AT1G09480 36.503 326 193 7 8 330 8 322 2.89e-56 186
MS.gene27553.t1 AT4G27250 33.234 337 204 7 2 322 7 338 3.09e-56 186
MS.gene27553.t1 AT1G09480 36.503 326 193 7 8 330 55 369 7.09e-56 186
MS.gene27553.t1 AT1G25460 36.615 325 191 8 10 330 6 319 2.47e-55 183
MS.gene27553.t1 AT1G51410 34.535 333 200 9 3 330 4 323 9.71e-55 182
MS.gene27553.t1 AT4G27250 32.530 332 203 7 2 322 7 328 1.67e-54 182
MS.gene27553.t1 AT1G09490 35.276 326 197 8 8 330 8 322 2.67e-54 181
MS.gene27553.t1 AT1G09510 36.533 323 191 8 8 327 8 319 3.78e-53 177
MS.gene27553.t1 AT1G66800 34.848 330 203 8 1 330 1 318 4.14e-53 177
MS.gene27553.t1 AT1G76470 36.991 319 190 7 5 321 4 313 8.75e-52 174
MS.gene27553.t1 AT1G15950 35.692 325 192 9 8 330 13 322 4.02e-47 162
MS.gene27553.t1 AT1G15950 35.692 325 192 9 8 330 13 322 4.02e-47 162
MS.gene27553.t1 AT1G09500 35.636 275 164 6 8 278 8 273 9.63e-47 159
MS.gene27553.t1 AT1G09500 35.563 284 170 7 8 287 8 282 2.42e-46 160
MS.gene27553.t1 AT2G33590 34.769 325 187 11 5 322 6 312 5.39e-45 156
MS.gene27553.t1 AT1G15950 35.144 313 187 7 8 318 13 311 1.27e-44 155
MS.gene27553.t1 AT1G15950 37.906 277 156 8 8 282 13 275 3.91e-43 150
MS.gene27553.t1 AT1G15950 37.770 278 157 8 8 283 13 276 4.54e-43 150
MS.gene27553.t1 AT1G80820 35.152 330 187 12 8 330 8 317 5.29e-43 151
MS.gene27553.t1 AT1G15950 37.410 278 158 7 8 283 13 276 7.03e-43 150
MS.gene27553.t1 AT2G33600 36.196 326 183 12 4 322 5 312 7.77e-43 150
MS.gene27553.t1 AT1G09490 32.180 289 184 6 45 330 12 291 1.58e-39 141
MS.gene27553.t1 AT1G80820 36.786 280 155 9 8 282 8 270 5.01e-39 139
MS.gene27553.t1 AT1G68540 36.486 222 129 7 113 330 1 214 3.95e-34 125
MS.gene27553.t1 AT1G09500 32.389 247 156 5 45 287 9 248 4.48e-32 121
MS.gene27553.t1 AT2G23910 26.562 320 204 12 1 307 1 302 7.80e-27 107
MS.gene27553.t1 AT2G23910 26.027 292 189 10 1 283 1 274 5.16e-25 102
MS.gene27553.t1 AT2G23910 25.862 290 188 10 1 281 1 272 7.80e-25 101
MS.gene27553.t1 AT4G30470 25.490 306 204 10 9 306 11 300 3.03e-23 98.2
MS.gene27553.t1 AT1G15950 31.718 227 145 6 106 330 6 224 4.06e-22 94.0
MS.gene27553.t1 AT5G14700 27.273 319 192 10 8 304 55 355 3.18e-21 93.6
MS.gene27553.t1 AT4G33360 26.056 284 185 6 2 281 9 271 2.11e-18 84.7
MS.gene27553.t1 AT4G33360 26.056 284 185 6 2 281 9 271 3.81e-18 84.3
MS.gene27553.t1 AT2G23910 26.054 261 166 10 58 307 24 268 1.19e-17 82.0
MS.gene27553.t1 AT5G14700 28.632 234 136 7 8 225 55 273 1.22e-17 82.0
MS.gene27553.t1 AT4G33360 25.806 279 182 6 7 281 2 259 1.65e-17 82.4

Find 64 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAATCTGTTCTTGAATT+TGG 0.142678 7.2:-30663592 MS.gene27553:CDS
AGTTTACTAGCTTTCTTATT+TGG 0.183198 7.2:-30663481 MS.gene27553:intron
CATTGAAAATATTTAGCTTT+TGG 0.214602 7.2:+30663938 None:intergenic
GAGCAATTATATCTCCCTTT+TGG 0.266827 7.2:+30663266 None:intergenic
AGAGAGCTTATCGGAGTTAA+AGG 0.285396 7.2:-30663055 MS.gene27553:CDS
ACTGGAGGTACAGGGTTTAT+TGG 0.300052 7.2:-30672547 MS.gene27553:CDS
ATACAATGGATGAAAGTTAT+TGG 0.306077 7.2:-30663684 MS.gene27553:CDS
AGAACCATTGATGGAGCATT+AGG 0.319772 7.2:-30663799 MS.gene27553:CDS
GATGTTGGCATTTGAAGTTC+AGG 0.343651 7.2:+30663191 None:intergenic
CATTTGAAGTTCAGGGTACT+TGG 0.365683 7.2:+30663199 None:intergenic
AATTGAATGTTGCAAGGAAA+AGG 0.371801 7.2:-30662945 MS.gene27553:CDS
AGCATCCATGAGTTTCTTAG+AGG 0.383422 7.2:+30663012 None:intergenic
TCATCTTCTAGAGAGCTTAT+CGG 0.385289 7.2:-30663064 MS.gene27553:intron
AAAGGAAGAGTTTGTGTGAC+TGG 0.394367 7.2:-30672565 MS.gene27553:CDS
GTACAGGGTTTATTGGTTCA+TGG 0.397157 7.2:-30672540 MS.gene27553:CDS
ATTGCTGCATTAAAACTCTC+AGG 0.400078 7.2:+30663901 None:intergenic
TCTTTCAGTTCAAAATCAAC+TGG 0.415670 7.2:+30663844 None:intergenic
GAGGTTAATTGCGGAAGCTT+AGG 0.430315 7.2:+30663031 None:intergenic
GGTACGAGATACAAGCAATA+AGG 0.431637 7.2:+30663349 None:intergenic
TCTTCAATAGTTGCAATGAA+TGG 0.434791 7.2:+30663242 None:intergenic
CTTGAACATCCTAATCCAAA+AGG 0.439060 7.2:-30663281 MS.gene27553:CDS
GTTTGTGTGACTGGAGGTAC+AGG 0.443705 7.2:-30672556 MS.gene27553:CDS
AATTCCTAATGCTCCATCAA+TGG 0.462695 7.2:+30663795 None:intergenic
ATTAAGAGACTCCTTGAAGA+TGG 0.464111 7.2:-30672514 MS.gene27553:CDS
TTCATTTGTCCTAAGCTACC+TGG 0.469552 7.2:-30663514 MS.gene27553:CDS
TAACAGCGTAACCATCTTCA+AGG 0.473218 7.2:+30672503 None:intergenic
TAGCCTTGACGAAATGTTTG+AGG 0.478982 7.2:-30662972 MS.gene27553:CDS
ATTTGGAGAACAACATGGGT+TGG 0.487832 7.2:-30663575 MS.gene27553:CDS
CTTGAATTTGGAGAACAACA+TGG 0.492029 7.2:-30663580 MS.gene27553:CDS
ATTGAATGTTGCAAGGAAAA+GGG 0.495429 7.2:-30662944 MS.gene27553:CDS
AAGAACAGATTTCTCAGCTA+GGG 0.495734 7.2:+30663600 None:intergenic
TCTAAGAAACTCATGGATGC+TGG 0.498821 7.2:-30663010 MS.gene27553:CDS
TTATTGCTTGTATCTCGTAC+CGG 0.499138 7.2:-30663347 MS.gene27553:CDS
GCAGCAATTGAAGGGTGCAT+TGG 0.503156 7.2:-30663886 MS.gene27553:CDS
TGAACAGATCCAGGTAGCTT+AGG 0.503818 7.2:+30663505 None:intergenic
CAAGAACAGATTTCTCAGCT+AGG 0.509193 7.2:+30663599 None:intergenic
ATGTTGGCATTTGAAGTTCA+GGG 0.512887 7.2:+30663192 None:intergenic
GTAGTAATGGAAGAAGAGAA+AGG 0.514620 7.2:-30672583 None:intergenic
ATTAACCTCTAAGAAACTCA+TGG 0.523086 7.2:-30663017 MS.gene27553:CDS
ACACCATACGACCAAGCAAA+AGG 0.525769 7.2:+30663631 None:intergenic
GCTTGTATCTCGTACCGGTA+TGG 0.528101 7.2:-30663342 MS.gene27553:CDS
AGTAAACTATGAACAGATCC+AGG 0.532869 7.2:+30663496 None:intergenic
AAAGGTCCTACAACAAAAGT+TGG 0.543969 7.2:+30663535 None:intergenic
GCATGTTGATGATGTGGCTA+GGG 0.545455 7.2:-30663318 MS.gene27553:CDS
TGTAGAACTCACTTGGATGT+TGG 0.557000 7.2:+30663176 None:intergenic
ATCATCAACATGCACCATAC+CGG 0.559899 7.2:+30663328 None:intergenic
ACCACTGTTAGAGCTGATCC+AGG 0.562994 7.2:-30672478 MS.gene27553:intron
TTGAACATCCTAATCCAAAA+GGG 0.565903 7.2:-30663280 MS.gene27553:CDS
TTGAATTTGGAGAACAACAT+GGG 0.580887 7.2:-30663579 MS.gene27553:CDS
AGACAAAGAAGAAGATACAA+TGG 0.599895 7.2:-30663698 MS.gene27553:CDS
TTGCTGCATTAAAACTCTCA+GGG 0.608456 7.2:+30663902 None:intergenic
GGACGCAATTGAATGTTGCA+AGG 0.609289 7.2:-30662951 MS.gene27553:CDS
CAGGGTACTTGGAAGAAACA+AGG 0.616116 7.2:+30663210 None:intergenic
CGTCCTCAAACATTTCGTCA+AGG 0.621284 7.2:+30662969 None:intergenic
TTTGTGTGACTGGAGGTACA+GGG 0.633409 7.2:-30672555 MS.gene27553:CDS
ATACACAGCAGAAGCACTTG+AGG 0.642464 7.2:+30663726 None:intergenic
TATGGTGCATGTTGATGATG+TGG 0.643007 7.2:-30663324 MS.gene27553:CDS
AGTTTCTTAGAGGTTAATTG+CGG 0.647629 7.2:+30663022 None:intergenic
TGCATGTTGATGATGTGGCT+AGG 0.650912 7.2:-30663319 MS.gene27553:CDS
ACCTGGATCAGCTCTAACAG+TGG 0.657128 7.2:+30672477 None:intergenic
CTGCATTTGGAAGATTTGTG+AGG 0.662737 7.2:+30663962 None:intergenic
AATCAACTGGAGTAGCTGTG+TGG 0.675600 7.2:+30663857 None:intergenic
GGTAGCTTAGGACAAATGAA+AGG 0.676303 7.2:+30663517 None:intergenic
GGAAGAGTTTGTGTGACTGG+AGG 0.702220 7.2:-30672562 MS.gene27553:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.2 gene 30662934 30672599 30662934 ID=MS.gene27553
chr7.2 mRNA 30662934 30672599 30662934 ID=MS.gene27553.t1;Parent=MS.gene27553
chr7.2 exon 30672479 30672599 30672479 ID=MS.gene27553.t1.exon1;Parent=MS.gene27553.t1
chr7.2 CDS 30672479 30672599 30672479 ID=cds.MS.gene27553.t1;Parent=MS.gene27553.t1
chr7.2 exon 30663482 30664009 30663482 ID=MS.gene27553.t1.exon2;Parent=MS.gene27553.t1
chr7.2 CDS 30663482 30664009 30663482 ID=cds.MS.gene27553.t1;Parent=MS.gene27553.t1
chr7.2 exon 30663188 30663389 30663188 ID=MS.gene27553.t1.exon3;Parent=MS.gene27553.t1
chr7.2 CDS 30663188 30663389 30663188 ID=cds.MS.gene27553.t1;Parent=MS.gene27553.t1
chr7.2 exon 30662934 30663075 30662934 ID=MS.gene27553.t1.exon4;Parent=MS.gene27553.t1
chr7.2 CDS 30662934 30663075 30662934 ID=cds.MS.gene27553.t1;Parent=MS.gene27553.t1
Gene Sequence

>MS.gene27553

ATGGAAGAAGAGAAAGGAAGAGTTTGTGTGACTGGAGGTACAGGGTTTATTGGTTCATGGATTATTAAGAGACTCCTTGAAGATGGTTACGCTGTTAATACCACTGTTAGAGCTGATCCAGGTAACCCTTTTTGAAAGTAAAAAAAACTATAAACTAATAAACTACATATTGTTATTGGATTTTAATGATATTATTTAATTTTCATATTGTTTAGTATCTCATTTGAATTAGAATTAAGTTGTACATCGTATTATTAAATTAATTCTTCTTTAATTGTTATTAAAAAAATATCTTCTTCAATTAAAAAAAGTTAAATTACCTTCTTAAAAAAAAGTTAAATTACCCGGAAAAAATAGAGATAAAAAAAATATGCCACTAAAAGACATTTGAATTACTTTTTTGAATTAACCAAGATAAAAAAATAGAATAAAAATATATAATACAATAAGACTTAAATAAGTTTTTGCTCTTTATAGATATATCATTTAAAAAAAATATATATATAATATTTTCTTTGTTAGCCGTCTAAATTTTTCTTCAAAATTTTTCTTCATCTTTTAAATGTTTTCGGTCAATATTTTTCATCTTTCTTTTAAGTCAACTCATAAGTATCCTTCAAAATAGATAAATTAAAGTATCCTTCAAATTTCTAAATTATTTTTTGCAGTGAAGTTTAGTAAATTATAAAAATTTCTCTTGGAAAAGTTTAGAATTTTTTAACAAAAGATAAATTAAATATAAATTTTTAAACATGAAAAGTACACATGAGTTCACTTAAAAATAGATATAAAAAATGTTATTGGAAAACTTTTTTTTTAAAGGAATTATTAGAAAAATTTGAATGGCTATTGTTTGAGAAAAAAATGAGGGATTAAAAATGAAACACATTTATAGGAATTAAAAAGTTATTTAACTCTAATAATAAAAAGTATTTTTTTATATATTTAAATAAACTAATATGACAGGTTTAAATAAAAATTGTCTATAATCCAGTCTTTTTAGATGAAACAGTTGTACCCTTGAAGTTTGGTTGGATAATGTACCCATGATCTAATTATACAATGTTATGTCATTTTTTGTATTAATTTATTTAAAGATAAAATCAAATTTTAAATGAGTTATGCATGCATGTGGGGTACGAGTACCCAACCAAACTTCATTGTATCTAAGAAGTTCCCTTCTTTTTAGCCATATTTCTAAAAAGAGCAATGCTATATCTCTAGAAAAATAACATCAAGAATTCTTCAAGAATAAATAGTAGCCAATCACAATGCACTATTTGCCTCAATTCAAGGAGGTGTGGCTGAAAAATAGGAAGTGGACAAATAAATCATCTAATTAAAATTTGCCAACTGTGTCTTGAAAAATTCTTGAAGCTATTTTTCTTGAGAAGTAGCATTTTCCTTCTAAAAAATACTTAGGTTTTGTATTTGAATGAAAGACTAATCTAACTCTAGTCTGACTTAGACAAAGATATAATAGACCCTATCGTTTGGTCGAACATATTCTCACCCAATATATTTTTTAAGAAATATTCTCATCCCTATACTATTAGTTATGAAGCACAAATTCCGACGTAGACACCTGACATGACACCGACACAGACACGTTGACACCACTAATGTAAACAACATAGGACACTGACACCACTACCTATATATTATTAATTTATTTATTAAAGATATAAACTAAGAATCAAAATACATATATATGCATCTAACTTGAGCAATTTATTTCATTAAATAAAGTTCTATAACAAGATACAATAGTATTTGATCTTTATTTATCCATCTACTATAGAAAAATTTAAAAAATGTGTTGCAATCAACTTTGATGTCTACACTAGTACAAAAAATACTTTATAGATTCGTGCTTAAGTCGGTTCGCTCTGCCACCCGTCTTAAGAAGTGACGCGGTGGCAATCTTGTAATTATTTTAAACTTTCTTAAGTCAGTTATAAGTTTACCCGACTTAAGGTTGATAGAATAATGAAACAAAATTATTTATAATTAATTTACTTAAGTTAGTCTGCTGACCACCCAACCTAAAAAAATTTCCCTTATTTTTTTGTCAAGATAAAAACTTTCCATTATTTTCTTTGTGAATCAATTTCCCTCTTTTATTTATTAATTATTAAATTATTAATTAAATAAATTAAATTTATTAATTAAATTTATAGTTATATATCACACAAGGTTTTCACTTTCTCTCTCTTTCTCAATTTCAATCGAACCAGACAAAGCTTCCTTTCTCACTTTCTCAATTTCAATTGAACCTCCATCACGCACAATCCTACAATCCCACACAAGCTCACTCACAATCGAACCTTCTTCACAAGCTCTCTCATTCAATCTAACCTTCGTCACTCACAATCATACAAAAGCTCACTCACTTCGTCAGGCACGCCGGAACCTCCGTCACTCACTCGATGAACCTTCCTCGCCACTCGCTCATTCACTCTCTCTGGAACCTCCGTCGCGCACGCCGGATTAAAGCCATCGCTGATGGAATGGAAGAAAGCCATCGCTGCCGGAACCTCCGTCACTCACCCTCTTCGTCATTCACTCTACCCCCAAAGGTATTTTTATTCTTTCATAATTTCTTCTATGATTTGGGAATTAGGGTTAACTGAAATTGTCATGTCTTTGAATTGTTTTGATGAAATTGATAATTCTAGAGCTTGATGGTTTTGATTTCTACGAAAATTTGGGATTAAGAACAGTGAAAACTTTCTGGGCAATAACCATGACTGTATGTGTTTGATTTGAAAAATATTTAGTGTTAGCTCATGAAATTGATTAGGTTCATATTATTAATTTTGATTTTCAATTTTGGATTTTGACTGGAGAAGAAGAATCACTCTGTATATTATGCTTTTACCGTGTAGCAGAATCTTTCTTAATATTAACTAGTGGAGAAAAACACCCTGAAGCCTCTAAAGGCCTCACAAATTAGTCATAACTCATAACACCCGTCACTATAACAATCACATATACCAGGACCCAACAAGTATAACAAGCAAACAAGCAATTCTCTATGGAGTTTGTTATACAAACAAGAAATTCAGTCTAAAGGGAACCATTAGTTACACCAGCAACATGAGAGAGCTCTGTTTAAGAATTATAAGTTTCCAAATGAGTACAAAGGAGTCCTCATGGGTATTATTATAATTGGTATTCATTTAGTATTCGCTAATTAGTAATTTGGTAATCCGCAAAGAATAAAGTACTTCTCAGACTATACCATGAGTTGAACGAGTAGTTAACTAACAAAGTATTGATATGCCAACACAGAATACACAAGTATATAAAGATTTGTGACCAATAACAATGATGACAATATTGTTTTATATATCTTTCCTTTTCTTTTACATTTTTTAACCGTATAGCAGAATATTTCTTAATACTTGCTAGTTCTTTTGTTTTGTATTTGTAGACTAAGTGTATGCGATAGTTGCGGCTGATAATTTGCTCTTCGACTAAGTTTCGTTTTGTGGTCTCATGATGTAATTTTATTGTTTGGTATAGATGGTTGTAGAAAATTGAGCTCATGATCTTTTTGGAGATTCGAGTTTCTTAATATCACTTGTTATATTTCTGCCTTGTAATGATTTGCGGTAATTTTTTTAAAGAGAACAAATGATTTTTCTGTTATATATTATTTGACATTATATGAATTTTCTGTTATGTTGACCTGCATTTGATATAAGAGTTCTATTGAGAAATAGATACAATTATTTAGTAATAATGCAAAGTAGCACCACATAGACTAAAACATTATAAGATCTCATGAAATTGTAGACAAACTACATGTATGGGATCAAACTACACTCAGATTTATAATGGACTTTAAAAATCAATTAGTTTAAAGAAAACCATGTATTTCATTAGTAGTATGTTTCTTAAATAGATCATAATGACTATTTCATTAATAGTATGTTTATTTAGGTATAATTGACTACTGTCAAAAAAAATAAAAGGTATAATTGACTACATAAAGCATACACGGGACAATTATTTATTTTCATGAATCTAATGGTTAAAAGAGAAATATTTATTTCATTAGTATAATGATGTGTCTCTTGAATACATTTCTAACAAAAGCAATGGCTATAATACTTTCTTCTTTCTAACAAAAGCAACTGCTATAATGCTTTCTTCCTGTTCTCTCCCACCTGCCCCAAACCATTCCATTTCTATGAAACATTCAAACATGTATTGTTATTGCAATGGGACACGCAACTGCCATGCATGATAAATCTTGCTTGGGAAGTTAACTTATTTTAACCATGTTAATCTTAATTTCAATTTCATTTTTTATACCTCAAAAAAGTTCTATATATTACGCTTCCATCATTACTACTTCACATTCAAACTCATTAAAAATACTCAACTCCTTAAGATATAGATACTCTTGTATACCTAGTGGCTCACATTTCTTTGGCATAGCTGCCTGTTCTCAGGGGCGGAGGGACCATGGGTGGCTGTGGTCCCCCCCAATTTTTCAAATTTTTTTTTTACTATTACCATACTACTATTATATATTACTATTTACACATGGTTATGAATATGGTCCCCTCATTATTAACATTTGTGTCTTTTTTATGTTGGGCCTTTGTCTTTTATAGTAAAAAAATCTTTCTACTTCTAAAAAAAAAGTCCATTATGTAAGTTGGAGCCCTCAATTCTATCTTTTATGGTAAAGACCTAATTAACTTTGGATGTAACTTAAATTTCTTATATATATAATGCATTGGAAGATTTAGTGTTTCTTTGCTAAAGTATACCTATTACGACTTGGTCCCCCAATGTATAAAACCTGGCTCCACCCCTGCCTGTTCTGGTTTTGCCTTTGCAATTTAGTTTCAGTGTCTACTTTTGGCGTTTTGGGCTAAGCTAAGGCTGGAATATTAATGATGATTTTGTCTTTAACTTAGCTTTGGCTTTTAGGTGTTAACAATAAATATAGTTTTCTGTACTGATATTGTTCTGAGCATCTAATTAGTAACCTTGTCTACTATCTTTGTTGTCGCAACTAGAAATTGCTTTATGTCTGCTATACTGTCAGGTTCAATGCTTTGAGCTCACAAATTTTTTTCTTGGCTTAATATGTTCGTCTCTTGTTTTAACTACTACTTATATAAGCTTTGTTAAATCCTTGTTGTAGTGAGACAATGTTATATTAAATAAACTGTGACTATGTTGCTTTCATTAAAATTTGTTGTATAAAAGCTCTATAATGAAAGGTTGGGGCTAGGGAGAGATATACTTTACATTTAAATAATAAACTAGTTGGAGATGGCAGGTTCAGTGCAACCTGTGTATGAGCACTAGTATTTGCTTATATGCAATTTTCTTATCATCTACTTTTCTGATCAGCCAATATACAGGGGAACATGTGGATTTGTATTCGACCCTGAAACATGTGAGCTGCAATTTTCGTGTTATGAATTGGTTATTATAGATATATACGTAATTGTATTTTGCCATTGTCATTATTATGCATAGTTTCATTTCAGTCTTTTGTGTATTAAAACGGTTCACTAATCCATATAGCTTTGAATTGAATTTTCTGATTGAGAAAATATCGAATAATAGAATGCCTTCTGATTGAGAAAACATCAAATAATAGAATGCCAAACTGTGTTTGCATGGTATGGGTCACCTTGCTTTGAGAAAAAATTGACTGAACATAGGTTGCAGGACAATTATGCATATTAAATTGGATGATAAATTTGATGTTGTAGGATTCTCTAGTGTCAGACGAATTTGTGCGAAATGGACGTGAGGACATCTTGGTGAAATCCCTTGGGCAACCTGAACACCCTGGTCGTGTTCGTGCTGCTGGTCGAGGCGTGGGTATTCGAGAATACTTTGGATCAAAATCTCATTCTACCCCATCTGTTACCAGTGATGCCCAACTAGCAGCCTTGACTAAGAAGATTACACAAGACGTGTTGCAGTCTCTTAACTCAACAAAATCAAAATTTCCCAATTTATTCTTCTAATACCACTCAACATGTTAGCACAAAGGGTAGTTGCTCTATTGTTCCTCAGACGCCAGATGATGAAGAGAATATCCCAGAAGAGTGTGAATTGTATATTGATGGAAGCAGTAATGTGGTGGCACACGCTTATGTGTACAACGTAGGGCCCACTATACACAATCAGGTGCTAACTAATGATATGGTTAGAGTGGCTGTTACCAAGGTGATAGATGCAACGACTCAAGTACCTGTGTCCACTAATGAGGTGACAACAATTGCTGAGGCCGTCAACACTTTTGCAAGTTATTTCTAATAAGGTATTTATTATATTTATGTCTATCACTATTTACACTTGATAGTGTTTAGACTTGTGTAAGGTTTTATCATAATGGTTTAGGATGGTGATATACCTATGAAAGTTGATGTTCCACCAAAAGAGTCGGAACTTATTTGTCAAAAATTGATAGTGAAGGCAATGTACATGGAACAAGCTTTGAAGTTTAAGGCTGGTCATAGTGGGATTGATATTGAGCTACAAAGGGGCGATATCATGGACTTGTGTTTGGGTAAAAAGGAATTACACTTGACTATTTTGCAAGTGTGGCTAACGTAAGTATATTTTATTTGTAAATTTAATGGTCATTCAATTACTTAATTTTATTTATAATGAAATTTTGTTTCACCATCTTAGGTATCTACAACGTCGTTGTGTTGAATTGGGCAAAAGTGGCATGTACGGAATGACCAAGTTAGTGTCAAACCTACATTCAAAACACGATGGACCATAATAAAAAGGATTTGTACCTAGCTCCTTACTTTAACAAGTAATTTTTCTCTCTCTTTATATCCAATCACTTGTAATATAGAGATTTGAATTTTGCAAATAATTTAATTGTTTAATGCTTGTAGTCTTCATTGGCAATTGCTCATATTAATCCCAAGAAGCGTGAGGTGACCTTTCTATGTTCGTTAGGAAAGAAGCCGGCAGATAAAAAAATATCAGCCATTGTTGATTTGTAAGTATTCAATTTATTTATTGTACATGAACTGTGATATGTTTAATGATATAAGTTTAACGATTAAGTATTAATTATTGTAGAGCTTTGGAAGATTATAATAAGCTACAATGGGTGAGAAAGCATAATAAAGTAGTATGGTTTTATCCCATTGTAAGTTGTTTACACATGCTACTGCTTCATCTATCATCTCTGTGTTGTTTCTGTATTAAATATCTTCTATTTTGAATAGTATTTACCGACTAAACGGTCGCATTTGATCATATTCGATTTCAGAGTCCAAGGCAATTAGTTTGTTATGAGAGCGGATATTTTGTCATGCTACATATGTTGAACATTGTATCCGATGGTTTTGTGGATTCATGTACGTATAAGATATCTTTAACAACTATTATAAATTAATTAATTTTATTTTTACATGTTTATATGATAAACTAATATTATGTCATCATTAATTTATTCAGTTATTTGCGGATTCAACTCCCTGTCAAAAGGATGAAGTTAAAAATGTTCAAGAACGCTGTTCAAATTTGATTCTGGAACTTATTGAAGCAAATGCGGAGTCATCTTAGTGAGAGACTGGTTTTTGGATTAGGTGGGGAGTAAGCTTTAGTGTGATACTGGTTTTTTTTAGCAGTTGGGGTTTTAGCTTAGTGTTAGTCCTGTTTTTGATTTTTTTTTACTATTTGCATTTTGGTTCTAACATGTACTTTACTCTGTCAGTGCAATGACTATCTTCTTTCGTATGCCAATATAGTTCTATAGTTTTATTTAATTAGTACATGTGGATTTTGGTTATGGTTTTCTCTTCAATGTATCTTTCTATTACTACAAGTGACATTAGATATTATGCATGTATGCAGTGGACAACAGGAAAACAAATAGAGCATATTCTGCATGAATGCTATATTAAAAAAAAATGAACTTTTTAAGTCGGTTAAAATTGGGTGACTTAAGAAGTTAATATGATGCCTTAGGACGGTTGTTAAATCTGACTTAAAAAGCAAATACTTCTTATGACGGGAGCTATTAGGTCAGACGCGTGCCTGACTTAAAAAGCCATTTTTAACCGTCTTAAAAAATGATGCCTTAAGACGGTTGGTAAATCCGACTTAAAAAGAAAATACTTCTTATGACGGGAGCTATTAGGTCGGACGCGCGCCCGACTTAAAAATCCCTTTTTAACCGACTTAAAAAGGGTTTTTTGTACTAGTGCTATAACCCCTCCTTGATAAATATTTCTCACACATCTTTAACTCTTGAATCTACGGTGTGTGGAAGCAGAGAAAAAAAATTCAGATATTTGTCTAAATTGTCCAACGAAGTGTTATATAGGTGTCGGACACCGGTCCAAAGTGTTAAAGTAAAAAAGTAAACAACTTATTTATGACACATATCGTACGAGTGTCATATGGTGTCAGGCACCGCGACACGTGTAATCCTAGAGATGTCCGTGCTCCATAGACCATATATTCTTAGGTTGTTTAATCAAGTGTGAATTTTGGTTATTTGTTGTTATTATAATAGTATTATGTTTTCCATGTAATGAATCTAACGATATCTATGTAAGCGAAATTATGTAAATAGCAGGGCAAAACAAAGATGTTAGCTTCCTCACAAATCTTCCAAATGCAGCCCAAAAGCTAAATATTTTCAATGCTGATCTTAGCTACCCTGAGAGTTTTAATGCAGCAATTGAAGGGTGCATTGGAGTATTCCACACAGCTACTCCAGTTGATTTTGAACTGAAAGAACCAGAGGAAATAGTGACAAAAAGAACCATTGATGGAGCATTAGGAATTCTAAAAGCATGCAAAAATTCAAAGACAGTGAAAAGAGTTGTTTACACCTCAAGTGCTTCTGCTGTGTATACGCAAGACAAAGAAGAAGATACAATGGATGAAAGTTATTGGAGTGATGTGAATATTCTTAGAACTTTGAAGCCTTTTGCTTGGTCGTATGGTGTTTCTAAAACCCTAGCTGAGAAATCTGTTCTTGAATTTGGAGAACAACATGGGTTGGATATTGTGACTCTTGTACCAACTTTTGTTGTAGGACCTTTCATTTGTCCTAAGCTACCTGGATCTGTTCATAGTTTACTAGCTTTCTTATTTGGTAAGTGAAAGTTTCCCATTGGTACGTTCTTGGATTATTTTGAAAGTATTTTCTTAAATAGATGAAAATTAACAAATGGAAGTTGCATTCAGGTGATATTGACAAGCATTCCTTATTGCTTGTATCTCGTACCGGTATGGTGCATGTTGATGATGTGGCTAGGGCACATATATTCTTACTTGAACATCCTAATCCAAAAGGGAGATATAATTGCTCACCATTCATTGCAACTATTGAAGAAATAGTTGACCTTGTTTCTTCCAAGTACCCTGAACTTCAAATGCCAACATCCAAGTGAGTTCTACATATTAATTACATGTCCTTCTCCATGTTAATAACTTGACTAAAATTATGTGATCTTTCTTTTATTGTTACTAATACTATTCAATTTTTGTTCATCTTCTAGAGAGCTTATCGGAGTTAAAGGTCCTAAGCTTCCGCAATTAACCTCTAAGAAACTCATGGATGCTGGATTCAAGTTTAAATATAGCCTTGACGAAATGTTTGAGGACGCAATTGAATGTTGCAAGGAAAAGGGTTATCTTTGA
Protein sequence

>MS.gene27553.t1

MEEEKGRVCVTGGTGFIGSWIIKRLLEDGYAVNTTVRADPAGQNKDVSFLTNLPNAAQKLNIFNADLSYPESFNAAIEGCIGVFHTATPVDFELKEPEEIVTKRTIDGALGILKACKNSKTVKRVVYTSSASAVYTQDKEEDTMDESYWSDVNILRTLKPFAWSYGVSKTLAEKSVLEFGEQHGLDIVTLVPTFVVGPFICPKLPGSVHSLLAFLFGDIDKHSLLLVSRTGMVHVDDVARAHIFLLEHPNPKGRYNCSPFIATIEEIVDLVSSKYPELQMPTSKELIGVKGPKLPQLTSKKLMDAGFKFKYSLDEMFEDAIECCKEKGYL