AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27554

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27554.t1 MTR_7g074880 96.626 326 11 0 1 326 1 326 0.0 649
MS.gene27554.t1 MTR_7g074730 78.049 328 70 1 1 326 1 328 0.0 546
MS.gene27554.t1 MTR_7g074870 90.559 286 27 0 41 326 3 288 0.0 540
MS.gene27554.t1 MTR_7g074820 76.292 329 75 2 1 326 1 329 0.0 527
MS.gene27554.t1 MTR_7g074850 75.380 329 78 1 1 326 1 329 0.0 526
MS.gene27554.t1 MTR_7g074710 70.245 326 97 0 1 326 1 326 1.57e-174 486
MS.gene27554.t1 MTR_4g023730 48.632 329 166 3 1 326 1 329 6.65e-106 313
MS.gene27554.t1 MTR_1g022440 46.729 321 166 4 8 326 8 325 7.08e-100 298
MS.gene27554.t1 MTR_1g022445 47.040 321 165 4 8 326 8 325 1.02e-96 290
MS.gene27554.t1 MTR_4g092080 39.222 334 189 5 1 324 7 336 1.21e-77 241
MS.gene27554.t1 MTR_4g081440 37.647 340 186 8 6 326 22 354 1.60e-75 236
MS.gene27554.t1 MTR_4g081440 37.647 340 185 9 6 326 18 349 4.01e-74 232
MS.gene27554.t1 MTR_5g056600 36.025 322 195 7 9 326 5 319 4.04e-68 216
MS.gene27554.t1 MTR_2g101330 39.877 326 180 10 7 326 4 319 1.82e-67 214
MS.gene27554.t1 MTR_8g062110 37.805 328 189 8 1 323 2 319 5.80e-65 208
MS.gene27554.t1 MTR_8g062110 37.500 328 190 7 1 323 11 328 7.02e-65 208
MS.gene27554.t1 MTR_8g062110 39.068 279 161 4 1 276 2 274 3.41e-62 199
MS.gene27554.t1 MTR_8g062110 39.068 279 161 4 1 276 2 274 4.06e-62 199
MS.gene27554.t1 MTR_3g005210 34.862 327 193 8 8 326 6 320 7.47e-61 197
MS.gene27554.t1 MTR_2g013230 35.838 346 196 9 1 326 1 340 5.09e-60 196
MS.gene27554.t1 MTR_8g062440 36.254 331 196 8 1 326 1 321 1.46e-59 194
MS.gene27554.t1 MTR_8g062440 35.952 331 198 7 1 326 1 322 5.04e-59 192
MS.gene27554.t1 MTR_3g031650 36.170 329 186 9 8 326 5 319 1.13e-57 189
MS.gene27554.t1 MTR_8g062440 38.351 279 163 5 1 276 1 273 9.44e-57 187
MS.gene27554.t1 MTR_4g077130 35.135 333 197 10 1 325 1 322 7.03e-56 184
MS.gene27554.t1 MTR_3g005170 33.436 326 197 8 8 325 10 323 8.39e-56 184
MS.gene27554.t1 MTR_2g013250 34.311 341 200 7 3 324 2 337 8.87e-56 184
MS.gene27554.t1 MTR_3g005170 35.531 273 168 5 8 277 10 277 2.98e-54 179
MS.gene27554.t1 MTR_5g072620 35.455 330 187 10 8 326 5 319 5.60e-53 177
MS.gene27554.t1 MTR_3g031650 37.226 274 160 6 8 276 5 271 4.77e-51 170
MS.gene27554.t1 MTR_2g013260 34.694 343 200 8 1 324 1 338 9.02e-51 172
MS.gene27554.t1 MTR_4g077100 35.231 281 173 7 1 277 3 278 1.10e-50 169
MS.gene27554.t1 MTR_2g104960 35.604 323 191 11 8 326 20 329 1.29e-50 171
MS.gene27554.t1 MTR_4g077100 35.587 281 172 8 1 277 3 278 3.00e-50 170
MS.gene27554.t1 MTR_4g009040 34.056 323 199 7 7 326 12 323 6.04e-50 169
MS.gene27554.t1 MTR_4g009040 34.146 328 196 8 7 326 12 327 1.06e-49 169
MS.gene27554.t1 MTR_7g117190 33.718 347 199 9 3 326 2 340 1.45e-49 168
MS.gene27554.t1 MTR_4g006940 33.956 321 199 7 8 326 17 326 1.78e-49 168
MS.gene27554.t1 MTR_4g006940 34.799 273 166 6 8 278 17 279 1.09e-44 154
MS.gene27554.t1 MTR_4g006940 34.799 273 166 6 8 278 17 279 1.09e-44 154
MS.gene27554.t1 MTR_7g117190 34.343 297 167 8 3 276 2 293 1.06e-43 152
MS.gene27554.t1 MTR_2g013260 34.915 295 171 7 1 276 1 293 2.02e-43 151
MS.gene27554.t1 MTR_4g009690 34.675 323 196 8 7 326 12 322 3.32e-43 151
MS.gene27554.t1 MTR_4g009690 34.056 323 199 7 7 326 12 323 6.08e-43 151
MS.gene27554.t1 MTR_2g028620 30.702 342 201 11 2 326 4 326 2.58e-41 147
MS.gene27554.t1 MTR_3g005170 30.061 326 177 9 8 325 10 292 1.88e-40 143
MS.gene27554.t1 MTR_2g028620 32.753 287 175 8 2 279 4 281 4.12e-39 140
MS.gene27554.t1 MTR_8g062110 33.929 224 134 7 105 323 19 233 1.12e-29 113
MS.gene27554.t1 MTR_2g013230 32.530 249 143 8 98 326 7 250 2.64e-29 113
MS.gene27554.t1 MTR_6g406250 30.827 266 150 8 5 253 52 300 1.12e-28 114
MS.gene27554.t1 MTR_5g029990 28.947 266 169 6 8 270 12 260 2.85e-28 111
MS.gene27554.t1 MTR_8g062110 34.857 175 106 3 105 276 19 188 1.02e-26 104
MS.gene27554.t1 MTR_0164s0070 40.625 128 74 2 7 133 4 130 1.12e-26 105
MS.gene27554.t1 MTR_6g406250 31.081 222 124 6 5 211 52 259 1.97e-24 100
MS.gene27554.t1 MTR_7g117190 31.915 235 130 8 115 326 3 230 1.43e-22 94.4
MS.gene27554.t1 MTR_2g013260 30.894 246 147 7 98 324 26 267 5.43e-20 88.6
MS.gene27554.t1 MTR_7g074865 92.500 40 3 0 1 40 1 40 1.34e-19 81.6
MS.gene27554.t1 MTR_3g101500 24.765 319 197 13 7 305 3 298 1.28e-13 70.9
MS.gene27554.t1 MTR_3g101500 25.600 250 159 9 7 251 3 230 3.08e-13 69.7
MS.gene27554.t1 MTR_3g101500 27.225 191 119 6 7 197 3 173 2.35e-12 65.9
MS.gene27554.t1 MTR_0685s0030 30.702 114 78 1 164 277 3 115 2.91e-12 63.2
MS.gene27554.t1 MTR_0685s0030 30.702 114 78 1 164 277 3 115 5.98e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27554.t1 AT2G45400 49.849 331 158 5 3 326 35 364 8.00e-112 329
MS.gene27554.t1 AT2G45400 51.228 285 132 4 3 280 35 319 1.90e-99 296
MS.gene27554.t1 AT5G42800 45.482 332 168 4 1 326 1 325 3.15e-94 285
MS.gene27554.t1 AT2G45400 48.621 290 142 4 43 326 19 307 4.71e-93 279
MS.gene27554.t1 AT4G35420 40.606 330 183 8 1 326 1 321 4.63e-81 249
MS.gene27554.t1 AT2G45400 52.727 220 98 3 3 216 35 254 1.94e-75 232
MS.gene27554.t1 AT1G61720 40.060 332 181 8 7 326 12 337 2.47e-71 225
MS.gene27554.t1 AT1G68540 36.957 322 192 7 10 326 6 321 8.92e-66 210
MS.gene27554.t1 AT1G09480 37.578 322 191 7 8 326 8 322 1.76e-62 201
MS.gene27554.t1 AT1G09480 37.578 322 191 7 8 326 55 369 4.67e-62 202
MS.gene27554.t1 AT2G02400 37.237 333 181 11 5 326 3 318 9.06e-62 199
MS.gene27554.t1 AT1G25460 36.449 321 193 6 10 326 6 319 1.06e-61 199
MS.gene27554.t1 AT1G09490 36.646 322 194 7 8 326 8 322 2.06e-61 199
MS.gene27554.t1 AT1G09510 38.820 322 181 8 8 323 8 319 5.43e-61 197
MS.gene27554.t1 AT4G27250 32.840 338 198 9 2 317 7 337 4.02e-59 194
MS.gene27554.t1 AT4G27250 33.030 330 198 9 2 317 7 327 4.83e-58 191
MS.gene27554.t1 AT5G58490 36.036 333 195 10 1 326 2 323 1.05e-57 189
MS.gene27554.t1 AT1G66800 35.890 326 201 7 1 326 1 318 1.35e-57 189
MS.gene27554.t1 AT1G51410 34.451 328 203 8 3 326 4 323 2.52e-57 188
MS.gene27554.t1 AT5G19440 35.276 326 191 8 8 325 10 323 5.19e-55 182
MS.gene27554.t1 AT1G09500 34.579 321 199 8 8 323 8 322 8.51e-54 179
MS.gene27554.t1 AT1G09500 36.264 273 165 6 8 276 8 275 1.72e-53 177
MS.gene27554.t1 AT1G15950 36.196 326 185 9 8 326 13 322 2.28e-51 173
MS.gene27554.t1 AT1G15950 36.196 326 185 9 8 326 13 322 2.28e-51 173
MS.gene27554.t1 AT2G33590 35.093 322 186 11 5 318 6 312 2.78e-50 170
MS.gene27554.t1 AT1G76470 34.568 324 205 5 5 326 4 322 1.40e-49 168
MS.gene27554.t1 AT1G15950 35.275 309 188 6 8 314 13 311 3.45e-48 165
MS.gene27554.t1 AT1G80820 34.268 321 198 7 8 326 8 317 3.94e-47 162
MS.gene27554.t1 AT2G33600 36.760 321 184 11 4 318 5 312 4.78e-47 161
MS.gene27554.t1 AT1G15950 36.996 273 160 6 8 278 13 275 1.23e-45 156
MS.gene27554.t1 AT1G15950 36.996 273 160 6 8 278 13 275 1.84e-45 156
MS.gene27554.t1 AT1G09490 33.220 295 188 6 35 326 3 291 1.94e-45 156
MS.gene27554.t1 AT1G15950 36.996 273 160 6 8 278 13 275 2.76e-45 156
MS.gene27554.t1 AT1G80820 35.531 273 164 6 8 278 8 270 3.30e-42 147
MS.gene27554.t1 AT1G09500 31.834 289 187 7 40 323 5 288 3.02e-39 140
MS.gene27554.t1 AT1G68540 32.877 219 138 5 112 326 1 214 2.91e-33 122
MS.gene27554.t1 AT2G23910 27.707 314 204 11 1 303 1 302 1.19e-30 118
MS.gene27554.t1 AT5G14700 29.560 318 182 10 8 300 55 355 1.55e-30 119
MS.gene27554.t1 AT2G23910 27.208 283 189 9 1 277 1 272 6.04e-28 110
MS.gene27554.t1 AT2G23910 27.208 283 189 9 1 277 1 272 7.27e-28 110
MS.gene27554.t1 AT4G30470 26.623 308 194 10 9 302 11 300 7.71e-26 105
MS.gene27554.t1 AT5G14700 33.014 209 107 7 8 197 55 249 4.59e-25 102
MS.gene27554.t1 AT1G15950 33.043 230 137 6 104 326 5 224 1.45e-24 100
MS.gene27554.t1 AT2G23910 26.136 264 175 9 49 303 16 268 2.13e-21 92.4
MS.gene27554.t1 AT4G33360 27.622 286 174 11 2 277 9 271 1.96e-18 84.7
MS.gene27554.t1 AT4G33360 27.972 286 173 12 2 277 9 271 2.20e-18 85.1
MS.gene27554.t1 AT4G33360 27.758 281 170 12 7 277 2 259 1.12e-17 82.8

Find 57 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCTGCATTGAAACTTTC+TGG 0.125713 7.2:+30653053 None:intergenic
ACCTCTCTGTCTTTGTTATT+TGG 0.159764 7.2:-30652633 MS.gene27554:intron
TATATGAAATTCAGGGTATT+TGG 0.255630 7.2:+30650742 None:intergenic
GAGAAGGCTGTTCTAGAGTT+TGG 0.265629 7.2:-30652744 MS.gene27554:CDS
GAGCAATTATATCTCCCTTT+TGG 0.266827 7.2:+30650809 None:intergenic
AGTGTTGGTATATGAAATTC+AGG 0.275539 7.2:+30650734 None:intergenic
ACTGGAGGTACAGGGTTTCT+TGG 0.280712 7.2:-30660379 MS.gene27554:CDS
CACTGCAACTCCAATTGATT+TGG 0.296688 7.2:-30653006 MS.gene27554:CDS
AGAACAATTGATGGAGCATT+AGG 0.319772 7.2:-30652951 MS.gene27554:CDS
GGCTTCCTCACAAAACTTCC+AGG 0.329690 7.2:-30653119 MS.gene27554:CDS
GTACAGGGTTTCTTGGTTCA+TGG 0.333463 7.2:-30660372 MS.gene27554:CDS
AGGGAGCTGAAGGAAATTAA+AGG 0.350467 7.2:-30650613 MS.gene27554:CDS
TCATTCATTTCCAAATCAAT+TGG 0.363586 7.2:+30652996 None:intergenic
ATGTAATGGATGAGAGTTAT+TGG 0.372531 7.2:-30652836 MS.gene27554:CDS
AAAGGAAGAGTTTGTGTGAC+TGG 0.394367 7.2:-30660397 MS.gene27554:CDS
GATTCAGTGTTGCAAGGAAA+AGG 0.394699 7.2:-30650503 MS.gene27554:CDS
TCTTCAATATTTGCAATGAA+TGG 0.401037 7.2:+30650785 None:intergenic
TGGCTTCTCTCGTCTCCATA+TGG 0.409487 7.2:-30650885 MS.gene27554:CDS
TTGCTGCATTGAAACTTTCT+GGG 0.416184 7.2:+30653054 None:intergenic
GTAGGGGACAAGAATCCATT+TGG 0.425747 7.2:-30650905 MS.gene27554:intron
ATTAAGAGACTTCTTGAAGA+TGG 0.426383 7.2:-30660346 MS.gene27554:CDS
CAAGTGCTTCAGCAGTGTAT+TGG 0.428274 7.2:-30652875 MS.gene27554:CDS
GTTTGTGTGACTGGAGGTAC+AGG 0.443705 7.2:-30660388 MS.gene27554:CDS
ATTGAATATGACCAAGCAAA+TGG 0.453024 7.2:+30652783 None:intergenic
TATCATCAACATGCACCATA+TGG 0.453095 7.2:+30650870 None:intergenic
AGTTTCAATGCAGCAATTGA+AGG 0.458381 7.2:-30653047 MS.gene27554:CDS
CTTGAGCATCCTAATCCAAA+AGG 0.458566 7.2:-30650824 MS.gene27554:CDS
GAGATTTGAAACCATTTGCT+TGG 0.459886 7.2:-30652794 MS.gene27554:CDS
TTCTAAAACAATGGCAGAGA+AGG 0.467203 7.2:-30652760 MS.gene27554:CDS
TTCATTTGTCCTAAGCGTCC+TGG 0.480298 7.2:-30652666 MS.gene27554:CDS
TAAATTGAGCCAGGACGCTT+AGG 0.480492 7.2:+30652657 None:intergenic
ATTCAGTGTTGCAAGGAAAA+GGG 0.509113 7.2:-30650502 MS.gene27554:CDS
TGGAGACGAGAGAAGCCAAA+TGG 0.520904 7.2:+30650890 None:intergenic
AGGACGCTTAGGACAAATGA+AGG 0.523329 7.2:+30652668 None:intergenic
GTGGTAATGGAAGAAGAGAA+AGG 0.524390 7.2:-30660415 None:intergenic
ATATTCAATTTCTAAAACAA+TGG 0.525829 7.2:-30652769 MS.gene27554:CDS
AGATAAAGATGATGATGTAA+TGG 0.533409 7.2:-30652850 MS.gene27554:CDS
ACACAGCCTTGAGGAAATGT+TGG 0.550570 7.2:-30650533 MS.gene27554:CDS
AAGGGTCCAAGAACAAAAGT+TGG 0.557011 7.2:+30652687 None:intergenic
TTGAGCATCCTAATCCAAAA+GGG 0.569459 7.2:-30650823 MS.gene27554:CDS
ACAGAGCGCAAGAAAGATGT+TGG 0.569599 7.2:-30653140 MS.gene27554:intron
CTAGAGTTTGGAGAACAACA+TGG 0.574839 7.2:-30652732 MS.gene27554:CDS
AGAGAGGTATAAATTGAGCC+AGG 0.580168 7.2:+30652648 None:intergenic
GCAGCAATTGAAGGATGCAA+AGG 0.592622 7.2:-30653038 MS.gene27554:CDS
ACTAGAACTCACTCTAGTGT+TGG 0.602184 7.2:+30650719 None:intergenic
ACCTGGATGAACTCTAACGG+TGG 0.616190 7.2:+30660309 None:intergenic
CTGACCTGGATGAACTCTAA+CGG 0.622324 7.2:+30660306 None:intergenic
GCCACCGTTAGAGTTCATCC+AGG 0.624738 7.2:-30660310 MS.gene27554:intron
TTTGTGTGACTGGAGGTACA+GGG 0.633409 7.2:-30660387 MS.gene27554:CDS
GTGTTGGTATATGAAATTCA+GGG 0.639563 7.2:+30650735 None:intergenic
CGATACGATTCAGTGTTGCA+AGG 0.647384 7.2:-30650509 MS.gene27554:CDS
TATCGTCCAACATTTCCTCA+AGG 0.653115 7.2:+30650527 None:intergenic
ACCAAATAACAAAGACAGAG+AGG 0.665667 7.2:+30652632 None:intergenic
AATCAATTGGAGTTGCAGTG+TGG 0.684696 7.2:+30653009 None:intergenic
GGAAGAGTTTGTGTGACTGG+AGG 0.702220 7.2:-30660394 MS.gene27554:CDS
GGACGCTTAGGACAAATGAA+GGG 0.710235 7.2:+30652669 None:intergenic
CGAGTTCAAACACAGCCTTG+AGG 0.724622 7.2:-30650542 MS.gene27554:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.2 gene 30650492 30660431 30650492 ID=MS.gene27554
chr7.2 mRNA 30650492 30660431 30650492 ID=MS.gene27554.t1;Parent=MS.gene27554
chr7.2 exon 30660311 30660431 30660311 ID=MS.gene27554.t1.exon1;Parent=MS.gene27554.t1
chr7.2 CDS 30660311 30660431 30660311 ID=cds.MS.gene27554.t1;Parent=MS.gene27554.t1
chr7.2 exon 30652634 30653158 30652634 ID=MS.gene27554.t1.exon2;Parent=MS.gene27554.t1
chr7.2 CDS 30652634 30653158 30652634 ID=cds.MS.gene27554.t1;Parent=MS.gene27554.t1
chr7.2 exon 30650731 30650923 30650731 ID=MS.gene27554.t1.exon3;Parent=MS.gene27554.t1
chr7.2 CDS 30650731 30650923 30650731 ID=cds.MS.gene27554.t1;Parent=MS.gene27554.t1
chr7.2 exon 30650492 30650633 30650492 ID=MS.gene27554.t1.exon4;Parent=MS.gene27554.t1
chr7.2 CDS 30650492 30650633 30650492 ID=cds.MS.gene27554.t1;Parent=MS.gene27554.t1
Gene Sequence

>MS.gene27554

ATGGAAGAAGAGAAAGGAAGAGTTTGTGTGACTGGAGGTACAGGGTTTCTTGGTTCATGGATTATTAAGAGACTTCTTGAAGATGGTTACACTGTTAATGCCACCGTTAGAGTTCATCCAGGTCAGTAATAAGTTTTTTTTTGTGTCAAATAACTTAATAGTAAGAGTTCATACATTTTAAATATAGAGATGAGGTATTTGGGGTTTGAATCCATCCCCTGCATATATTAAACGATGTCTCTATTTAACCAACACAAGTTGGCTCAGTTGGTAAGGAACGGGCCACTTCCTTGCAGAAGGTTGTGTGTTCGATTCCCTCTGCCGATGTGAGGGCAGTGTTGGTGGGTGATCTGTAAGTACCTCTATAAGTGCCCTTTGGGCCCGGAGGCGTGCCCTGACCCTGGTGGTGACCGGAGCCGAACCCACCTAAACTTTTTTTCATCAAAAAAAAAAAAAACGATGTCTCTACCAATTGATCTTCGAACTCCATTTCCTTATGAATAAATTAAATAATAACGAGTCCTTTGAATTTTCATTTTTTTTTTCATGTACTTATAAAAAAGTAGTTTTTCGTGTTGCAAAATTGAAATTCTTCCTCCAATAATAAAAAGAAAAATTAAAATTCTTCCCTTCCTCTCTTCAACTCGCTGCCTCAGCCCTAAAAATAATGTAGGAGTGTTCATATCATTTTCATATTCAATTTAATATAATGTGCAGAAATGGATTAATAAACCCAAAATAAACAAGATAAAAATAGGAAAACAAATAAAACATTTTTTAAAATGGATTGGACCAATTTTGAGTTCCTAAAGTGAAAACATATCCAAACAAAAAGTTAGCCATATTGTTGACAAAAAGGTGCCATGTCTTTTTTATAAATTATAAATCCAACTGTCGCGTCATTTTTCGGAGATCAAGGCTATTTGATTAGCTTTTGACTCACTAATTTATTTCATGACTGAACATTTAATTTTTTTTAAGAAAAGGGGAAAAAAGTTCAAACACCCCATATATTGAAAAGATTTAGGTTCGGGGGTTGGTTACATAAAGGGAAGGTGTTAGCACCCTTTATGTCCGTAGTACTCTACGGGAACCTTTTAGTTTTATTTAAATAATGTGCTATAACTTGCTTATTTTGAAAGAAAAGGAATTTTAAGTGATTAAAAAAAGGAGGACCTAATTATCTACATTTTATTGATTTGGAAGGACGAGTGTCCTCTGCCTACGTACCCGTGAGGGATCAAAACCTCGTAGTTCGGGGTTAAAATTGACGTTTGATTGGTTGAGTGTTTTTTATTATTTTTGAAAAAATGGTTTAAAAGAATGAAAGAAAGCCAAAGTGGCAAAGTGAATTTGTTTGTATTTTTTATATTAAAGAAAAATGACTTGAAATATGATTAAATTGATTGAAGTTTGATTAAGGAAAGAAAATAAAAAAAAGACGTCACTTGATTTGAGATCAAGGTTTATTTTAAGAAAAATATTTTTGTGTTTTGTTTTTGTTGTTTTTTGAATGATAAACTAACGGAGCATAAAAAAGGTAAAAGCGTAGGGACCTTATGGCGGCGTTGGCCCACCACAAGGACCTTCTACCTAAACTAGCAAGTGTAAAAAGTAAACATGCACCCACACACGCACACATGCACCTAAACTATTACAAAAAAACCTATTAATACATTCTATGGTCTGCGAGAAGTAAATGACAGAAAAATAAATATGCATGATTTACATTAAGCCTATATTTACATTCTAACATCTTGCTGTAAAATAAATGATAAAATAAATTAAAATGCGAAATGATCAAACTAAACTAGAAAATGCATGAATGAATTACATTTAAGCCTAACTTATACATTCTAAAAAAATCTATGCGATAAAAATAAATGACAAAAGAAAATATGCATGAATGATAGTAAATTTGTAGAACAATAAACCTAGTACTAGATTAAAATGCATGAGAATTAAAAAAATGCAATGCTAACGAAAAGACTAAAGTGCGTGAAATAAAATACAGCAGGGGAGGTAAACAGTGATCAAGGGGATGCAGAACTTAAAATAAGTGAAATTGGGCCTTAGGAAAGCTGCAGGCGCTGGGCTTTAAATACTGGATTCAGACCGTCTGATTGAGATCAGACGGTCTTGAGGATGATTAGAGGGATCTCACATGGGCCAATGGATCGTATCTAACAGTCAAGGATGCGTGGGCGCACGGTAAGGCTATGTAACCACGCACATGCGATCACAAGGCCTATAAATAGGCAGTAATCAGGGTTTTCACTCATTTTTAGTCCTCTCTCTCTCTCTATCTCAGTTCTCTCTCTCTATGTCTTCTTCTTCTCTCAACTTTTCGGCGAGAAGCAAGTACACCGGTGCGGTGGTTGGCCGGTCGGAGTGGCGGCGCCACCGCACCGGACTTTAAAAGTTCCTCTTTTTATATTTTTTTTTTAGCTCTTTCTCTTCTACTAACCTCCCTCTATTCAATTTCAAACTTAAAATTCTCAGAGAACAAACGTATTGTCCTAGATCTGAACAACAAAATAAAAAGAGAATACCTTCCGTTACCGTACACGTTCGTACCACGGTTTGGCTGCTGTCACGGGCGTTGTGGTGATTTCCGTCCGGTTCTGTGATGCTTGTTGGGTTCTGGATGTGCTGTCTGTTGACTGATACAGGTTCGGACTCTGGCTGCTTGCTGGTCGTCGGTGTAGGCTGTGTTGGTTACGTTGTCGTCGTAATCGATCCGAGGCATGAATGGCTCCGCCTTGCTAGTTGTTGAGTTCGGTTTGATTCATGGCTACGGTTCAGTGTTGCATTTGTGGATGAATCGGTGGTTGGAAAGGAAATTTGAGGTTGGTTTGGTCACAGATGTGTTGGGTTGTGGAATTGGTTGCCGTTATTGTCGATCTGGTATTGGATTGGTCGATTGGCTTTTGTGTTGTCTGTGTTAGAAAATTGTAGGTTTTTGTCTCTGAGAATTGTTTGTGTATTATGATATATGATACAGGTTTATGTGCGTTGAAATTGTTGTGTTCTGCAGATGAATTAGGCGTGATTCATTTGCGGGTTGTGCTGGAAATTTCGGCCCCCTCAACTTGTGTTTTGCAGGTTGTATATATAGTGAGAAGCTTTGGATGTTAGGGTTTGTTAAAAAGATGCACAAAAACTGGCTTTGGATTGGAATGGATTGTCATTTTGTTCCTGTAAATGTGTCTCTGCAGATGAGGCTGAGAATGATGAGGCGTGACAGCTTACTTTGAACTTGGACAATTTTTTAGACAAAAAAATATAAGACTAAAAAGAGATAAAGATTAACATAAAGGAAAAGGATTAACATAAAGAAAAAAAGATTAAAATTAATTGAAACCTAAAATGAATTAAAATTAGTAACAAGAAATTTAAATAATAATAATAATAATAATAGTAATAAGATAACTATGCATATTTTTTTTGGGATTTTTGACTTATCTTAAAAAAAGAAGTAATTAACTAAAAAAAGAAATGAATGACTAATTAAAATATTAATTGATTAAAAAAATAAAAGATGGAAATAAAGATAACAATAAAAAAAATTGAGAGAAGAGGACCCGACTCGGAATCAAATATGAGGTATTTTAAAAATACCTCTCTGTCGAATTTTTGACTGAAAAATCAGAGATCGGTCAGGGTCAGGCGACGCGTGTCAGTGGGACCTTAGGTCAAAAATTGGGGTATGACACCAACAAATGCCGATTCATGTCTTAGTTCGTTGGAAGTATAGTAAAACTAGATATATAGAAAGAAAAAAAATTTAGAGAGAGTTTGAGTGGGATCTATAGTAAAAAAATGTTGAAAGATATGTTTAGGTGATTTAGACAATATACACTGTGTTTGGAACAAGAGAAATAGAGGAGAGAGAGCAATGTGAGAAAGAAATAGGGAAGAGAGAGTAAAGCCATTGTGTATGGAACAAGAGAGATACAAGAGAGAAAGACTAAAAGTTGATGGGTTCCACATAAAAATCAATCTCTCCATTGATGTGGAGAAAGAGCTTCAACCTATGGTTTTCCATTTTAAGCCTCATAAAAGATAAAAATTAAATAATATAAAAAAAATCTGTTTTACAAGTTAAAGGATACTATTATCAATTTAAAATACAATTCTACTTCTCTCTTCTCTTTCTTTTTATACAAAACAATACATCAAATCTACTCTATTTTCCATCTTTTTCTCTCTTCTCATACTCTCTCTTACCTACTTCTCTCTTCTCAACTTCTCTTCCAAACCAAACATATACTATATAACATATACTATATAGATAGTAAAAAAATGATGAAAAATATGTTTAGGTGATTTAGACATAGAGAGAAGATACGGAAAAACATCAAGATAATATTTAAAAAGATGTAGAGATTAAGAGGTTAACGAGATTTTTTTTTTTTTAAAGAAGGTTAAGGAGAATGTTAAATCGTGTTCCACATACATTGATTAAACAACTAAAAGAAATAAGTTTGTATTAAAAAGTGATATAATTATTACTTGATCAAAAGTAACTGCGTATATTTTCAAGATAAATTTTTTATTTTTGGATTCTTTAATCAGTGTCTTGGAGACATTGGTTAACAAGATTTATTAAGTTGAACCTAAGAGAAATGATATTTGAACATTATGTTTTTACTTTCTCTCTATTGCTTTGGTTTTTATGTCAATATCTCACTTTCTTTGTAAAATTTTGGTTGTATAAAAAATTGTCTTTTTTTTTTTGTACAAAAAGAGGGCAGCGCCCAAGAAAAAGAAAAAGAAAACTAAGCGATTAATCAAACATTCTAGGCATGCAATCCCCAGAAATTGTCATTTAAATGAATGTTCAAATAACACAACTCTGAAGCTAAATATAGTCCGCGATAAAACATTCTATCATTATTTTATCCATTTAGTAGGGTTGTTGTTGCATAAATCCAACAAAATGTAAGAAAATTGATGTTCATGTTGCGAATTCGATGAGAAATCACACCAATAACAACTTGATGTGTGGAGCAAGGGATAATTAAGCAACCAAAATCAAAGTGTATGGAACAATTATACAAGCCAAAAGTAGAAAACAACAGGAGAAAAGAAGAGAGAACACAAAAGAATTTGTTGACCCAGTTCGATCCAAATTGACCTAGTCTGGGGGAGAGAGCAGCCCTCCGTTTCACTATATGATGAGTAACGTTACAAAAGAGATATCAATGGGTTACAAGAATAAGTCTCCCGCCTAATTCTACCCAAATCCCAACCTCTTCCCGTAACCAAGAGACTCAATCCTAATAGTGTTTCCCAAGGTGAATCAACCTTCAAACCCTAGTTTGTTGCCAAGAGACAAACTCTATACAAACCCTAGTTTGATTTCTAAACCCTTGTTTCTGCCCCTTCTATCTCCAGGTTTACACTGATACAATGTCTATATTTATAGTAAAAGAATCCTCAATAAAACTGGAACAAATCTAGGCCCGAAAATACGTTTCAGTTTTATTTTCTGCTGTCAAAATCGGCCACCGTGCTCACCAAATCGGCCACCGATTTTTACAGAATCAACAGACAAAAAAAACATAGTCCAAAATCGGCCACCGTGCTCTGAAAATCGACCACCGATTTTCAACTTCCAGAATTCCAATTTCTTCAATTTTAAGGCACTTTCACAACAGTACACACTTCTTAGTTCGCTGAAAAACACCCAATAATTACTCAAATGCCCCATCGACAATTAACCGCCGATGGCTCTTTATGGCGGCAGTTAACTGCCGATCCAAACATGTATACATGAAAATGTCCATCGACACTTAAGTGCCCTGGAGAGAAAATTTCTCACATCTTATGACAAAAGGACAAGGATTTTTCAGATCTGTTTGGTAGCTGAAACATTTATTGATTCTAGTAACAATTTTGTTTTGGTTTGGCTTGTGGAGAAAGAAGTAGAAGAAGCAAACCCATCATGGGAGATGGTGTTGCAGTGACACCTCTGCAACCATGATAGAAGCTCCACCATAAGATAAAAATTCAATGAATTTTGATTATATGAGTGAACTTTTGTTAGATGGCTGTTGGTTGGAAGCTTCTCCCGATGGATCTGAGTTCTTGCTGCAAAGTTCTCCTTTTTCAAATCCAATCTTTGACCCTTCATTCTCTTAGTCTGCCTTGGAGAACAACAAACCCTTCAGATTTTATGCTACACAACACTTAAGTACCGATGGAGTTTTTCATGTGCACATGTTTGGATCGGCAGTTAACTGCCGCCGCAAAGAGCCATCGGCGGTTAACTGCCGATGAGGACATTTGAATAATTTTTGGGTGTTTTTCAGTGAATTATGAAGTGTGAACAGCAATTTTCAAATATAATCCCTATGTTCAATCTCTAACACACCCATCACACATGCATCCCATAGTGAGTGGTGGTCAAACAACATATATAAGATTATAATTTAGTATTTGACCAAAAAAATTAGTAGTAACATTTTAGAAATTTTTTAAAAATAAAAAATTAAAAAATTATTGTGTATATGTTTGTCATAATAAAAACCATTTTTTTAAAGAAATTACATATAAACGAGTTTAAAAAATTTCTAAAAATTGCTTCTAATTTCTAAAATACTATTTTGGTCGAATAACAAGTTATTATTAATGAGATCTCCATCGACAGATATTTATTCTAGTAGAAAAGGTTAGATTCTTACAACATAACAAATCAAGGTTCATATCTGAGTTGTAAGAGATGTTTGCATTTAATCTCTAACATGTGTCAAATATGATTAACTCCAGGGAAAAAAATTATGGAAGGAAAAAAACTAGCCATTATGACATCTCTAACTAGTCTTATGTTATACTCCCTCCGTTTCAAAATGAGTGTCGCTTTAGCCAAAAAAATTTGTTTCAAAATGAGTGTCGTTTTCAGTTTTCAATGCAATAATAACTTTTTCTTTTCATTTGTACCCTCCAATTAATACTCTGCACACTACTTTCAATGTATTACTTTTAAAACTAACCAATAGTTAATAAGGATAATTTGGTAAAATAACCATTCTCTTCCTCTTAATCATTGCATTTCTTAATATGTGTGTAAACACCTAAAACGACACTCATTTTGAAACGGAGGGAGTAAAGAAATTTTTTAGCTGTTTTAATTATATGTCTTAATATGCATGAAGTGTTAATTCAGCGGTAAGAGTTTAACTATTAGTGCTACATTAATTATTGATAATTTTTCCTCCTCCAATGTATACTTGACCAACAAGCAATAAAATTGTGTTTCATATAACAGAGCGCAAGAAAGATGTTGGCTTCCTCACAAAACTTCCAGGTGCATCCCAAAAGCTAAAATTCTTCAATGCTGATCTAAGCATCCCAGAAAGTTTCAATGCAGCAATTGAAGGATGCAAAGGAATATTCCACACTGCAACTCCAATTGATTTGGAAATGAATGAATCAGAAGAAACAGTGACAAAAAGAACAATTGATGGAGCATTAGGAATTTTAAAAGCATGCAAAAATTCAAAGACAGTGAAGAGAGTTATTTACACTTCAAGTGCTTCAGCAGTGTATTGGCAAGATAAAGATGATGATGTAATGGATGAGAGTTATTGGAGTGATGAAAATCTTCTTAGAGATTTGAAACCATTTGCTTGGTCATATTCAATTTCTAAAACAATGGCAGAGAAGGCTGTTCTAGAGTTTGGAGAACAACATGGTTTAGATGTTGTGACTATAATACCAACTTTTGTTCTTGGACCCTTCATTTGTCCTAAGCGTCCTGGCTCAATTTATACCTCTCTGTCTTTGTTATTTGGTAAGTGTTATAATTTTTTCTAGTCCTTTTTTTTCTTCTGACTATATTTGTTGAGATTGTTTTTTTAATAAAAATTGGGGAAGAGGAAGCTATTGTTTATAAATGTGGAACTAATGATCATTTTTCAACGGACTCAAATGGGATTTGAACTCCATTTTTGAGGTTATAAAGTCAAGTTTTTACCACTAAGTTGACATCTAATGCATACTCCCACCGTTCCAAAATGTAAGCAAAAATGAGTCAATATAAATTGATATATTTGGTACAAATTTTGAATCAAATACATTAACTTTTGTTGACCAATTTTTGCTTATATTTTAGGACGGGGGAGTATTTGTTAAGAAGTTGAGTTTCACACTATGAAAAACCATCAAATTAGCTTTGGAAATGAAAGAGAGAGAAGATAAAATTGGTCTTCAATGTTAGTGTATCTAATTGTGTGGATTAGGGATCAGTTGGTCAATTAGGTGATTAAGTGATTGTTGAGTATGATGACTTATAGGTACTGAGAAGTGGGAAGGATTGAATGAGCGAAGAGAGTGAGACCTGCCCTTAAACTTGAGCCAAACATTTGTTGTGGGGACGACGTCCCCGCGGTACAGTTCAATGATGCTATAGTTGGACCGCGGTATTTAAATACAAGTTGGATATTGTAAACCATAATCTTTTCACTAATAATACAAATGAATTATAGAGAGACATACACACGATTAACCCAACTTCACTATCAAACATCTTGTGTTCTTTATTTGCGTTCATCATTTCTTTGCTCTTTTAATTTAGCTTGTTGTTCTAACGGTAGTCAACATGAATTGATTGTAATTTTTGCTTCAATTTTCAACCACTATATTTTACGACTTATCATAGAAATCATGGTTAGGCCTAACACAACCAGCTTGTGAGGTGAGGATTGCCTCCACTTATAAACATATTGTTAGGCCATCTCCTATCCGTTGTGAGACTCTTAACACACCCCCTCACGACCAACACTATTGGGCTTTGTTCGTGGACATAAATGGTGGGTGGTCTGATAGCGGAAACCTGATGGCAGGTGGCCCGATGGATTTTGAAGAGACTATGATACCATCTTAGAAATCGTGGTTAGGCTTAACATAATCCTACAAAACCGACTTATGTGATGAGTATTGCTCCCGCTTATAATTTATAAACACATTGTCAGACCATCTCCTATCCGATTTGACACTTTTAACAACTTATTAACCTCATCAAAATTGCAACTGGTTGATTGGAATGTGTAGTATATGTAATGTTTCTTTAGTAATAAGTATTGTCACAATCGTGAATTTTAGCTTTACTATGTCAACTTATAATATTTTATAAGTATTGTCATTTTCTTGAATTTTTCTATCTAATTCATCAAAACACTAAGTATCACTCCCAACACAATACTGACAAAATATTAGTGTGATACATATAAAAAAAACATTTGTTGGAATATGTCAGGACCTTAAATGAATCTTAGTTTCCTTTAGAAAATTAGTTTATGCAGTTGTGTGGAAGAATACATTTGGTTTTTAAAAAAAAAAACAACAATATTAATATTGTCCGTTAAAAAAAAGTATTGTCATTACTTTGATTTTCCCAATCTAATATGTAGAATTCACAATGGATACCAATAAATTCATTTTTAGGCATACCTCTAGTTTCATTGTAAAAATGCTAATTAAGAATAGAAAATGGATGTTGTAGGGGACAAGAATCCATTTGGCTTCTCTCGTCTCCATATGGTGCATGTTGATGATATTGCAAGAGCACATATATTTCTGCTTGAGCATCCTAATCCAAAAGGGAGATATAATTGCTCACCATTCATTGCAAATATTGAAGAAATAGCTCAACTTATTTCTGCCAAATACCCTGAATTTCATATACCAACACTAGAGTGAGTTCTAGTGTCATCTTTTATAAAAAAGATTTTTTATTAGATTAATGTGATATTTCCTTTTACTGTAACTAGTATTTATTCATTTTGTTCATAGGGAGCTGAAGGAAATTAAAGGTGACAAGCTTCCACATTTAACCTCAAAAAAACTCATGGATGCTGGATTCGAGTTCAAACACAGCCTTGAGGAAATGTTGGACGATACGATTCAGTGTTGCAAGGAAAAGGGTTATCTTTGA
Protein sequence

>MS.gene27554.t1

MEEEKGRVCVTGGTGFLGSWIIKRLLEDGYTVNATVRVHPERKKDVGFLTKLPGASQKLKFFNADLSIPESFNAAIEGCKGIFHTATPIDLEMNESEETVTKRTIDGALGILKACKNSKTVKRVIYTSSASAVYWQDKDDDVMDESYWSDENLLRDLKPFAWSYSISKTMAEKAVLEFGEQHGLDVVTIIPTFVLGPFICPKRPGSIYTSLSLLFGDKNPFGFSRLHMVHVDDIARAHIFLLEHPNPKGRYNCSPFIANIEEIAQLISAKYPEFHIPTLEELKEIKGDKLPHLTSKKLMDAGFEFKHSLEEMLDDTIQCCKEKGYL