AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27992

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27992.t1 MTR_8g089650 97.950 439 7 2 1 437 1 439 0.0 875
MS.gene27992.t1 MTR_4g104410 54.335 346 136 10 88 426 33 363 7.62e-115 341
MS.gene27992.t1 MTR_5g035980 46.959 411 156 14 67 428 48 445 1.43e-106 323
MS.gene27992.t1 MTR_4g106480 45.455 396 181 12 51 428 20 398 5.44e-103 312
MS.gene27992.t1 MTR_3g078380 46.173 392 178 13 60 428 30 411 6.36e-102 310
MS.gene27992.t1 MTR_1g021590 42.687 335 172 9 99 427 112 432 4.21e-80 255
MS.gene27992.t1 MTR_2g060070 39.710 345 190 8 82 424 51 379 1.66e-76 244
MS.gene27992.t1 MTR_5g018520 40.541 333 180 7 99 426 100 419 3.66e-76 245
MS.gene27992.t1 MTR_4g068457 37.883 359 204 7 79 427 108 457 9.15e-74 240
MS.gene27992.t1 MTR_2g101410 33.062 369 205 11 77 428 66 409 8.45e-59 199
MS.gene27992.t1 MTR_3g110185 36.441 354 195 12 85 428 78 411 4.89e-54 193
MS.gene27992.t1 MTR_3g110185 26.275 255 149 8 209 439 87 326 7.85e-12 67.8
MS.gene27992.t1 MTR_4g071130 24.290 317 169 11 78 366 2 275 2.60e-13 72.8
MS.gene27992.t1 MTR_6g465150 26.316 228 117 6 230 424 129 338 6.39e-13 70.5
MS.gene27992.t1 MTR_1g090827 24.606 317 168 11 78 366 2 275 7.96e-13 71.2
MS.gene27992.t1 MTR_3g088545 23.618 199 97 3 218 367 95 287 1.16e-12 69.3
MS.gene27992.t1 MTR_8g028700 27.354 223 126 9 209 424 230 423 1.38e-12 69.7
MS.gene27992.t1 MTR_2g102267 25.260 289 163 11 160 431 41 293 2.42e-12 68.2
MS.gene27992.t1 MTR_4g105700 27.074 229 134 10 222 429 419 635 1.13e-11 67.4
MS.gene27992.t1 MTR_1g072140 27.660 235 141 9 200 429 18 228 1.73e-11 65.5
MS.gene27992.t1 MTR_4g123597 25.857 321 186 12 73 374 414 701 2.39e-11 66.6
MS.gene27992.t1 MTR_4g069090 31.250 160 95 5 209 367 109 254 8.23e-11 64.7
MS.gene27992.t1 MTR_8g104010 27.523 218 132 7 209 425 912 1104 9.54e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27992.t1 AT1G24530 64.130 368 118 4 62 428 51 405 5.21e-168 479
MS.gene27992.t1 AT1G24130 46.053 380 175 12 67 428 45 412 2.02e-100 306
MS.gene27992.t1 AT5G50120 42.391 368 180 11 85 428 27 386 6.91e-91 281
MS.gene27992.t1 AT2G26490 41.493 335 176 9 99 427 99 419 7.84e-79 252
MS.gene27992.t1 AT1G49450 40.708 339 183 7 99 427 137 467 8.21e-76 244
MS.gene27992.t1 AT3G51930 38.292 363 182 11 84 424 50 392 2.30e-74 239
MS.gene27992.t1 AT3G50390 39.645 338 182 8 99 427 101 425 6.00e-74 239
MS.gene27992.t1 AT3G18950 38.393 336 189 8 99 427 141 465 7.40e-71 231
MS.gene27992.t1 AT4G34380 37.389 337 196 7 99 427 128 457 1.26e-65 218
MS.gene27992.t1 AT1G47610 39.763 337 179 12 99 426 27 348 1.31e-65 214
MS.gene27992.t1 AT1G21651 31.707 164 100 5 209 366 523 680 2.06e-14 76.3
MS.gene27992.t1 AT1G21650 31.707 164 100 5 209 366 523 680 2.97e-14 75.9
MS.gene27992.t1 AT1G49040 28.899 218 129 7 209 425 898 1090 1.39e-13 73.6
MS.gene27992.t1 AT4G02730 29.534 193 108 9 241 429 34 202 1.28e-12 68.9
MS.gene27992.t1 AT3G49660 26.316 209 125 7 222 424 127 312 2.70e-12 67.8
MS.gene27992.t1 AT1G62020 23.975 317 170 10 78 366 2 275 5.59e-12 68.6
MS.gene27992.t1 AT5G25150 33.103 145 86 5 225 366 477 613 1.21e-11 67.4
MS.gene27992.t1 AT5G25150 33.103 145 86 5 225 366 477 613 1.47e-11 67.0
MS.gene27992.t1 AT3G21540 30.872 149 92 5 229 374 561 701 2.37e-11 66.6
MS.gene27992.t1 AT3G05090 29.412 204 94 9 209 366 83 282 3.37e-11 65.9
MS.gene27992.t1 AT3G05090 29.412 204 94 9 209 366 83 282 3.37e-11 65.9
MS.gene27992.t1 AT3G05090 29.412 204 94 9 209 366 83 282 3.37e-11 65.9
MS.gene27992.t1 AT5G16750 25.751 233 137 10 219 438 415 624 4.13e-11 65.9
MS.gene27992.t1 AT5G16750 23.293 249 149 6 85 326 10 223 5.60e-11 65.5
MS.gene27992.t1 AT2G21390 23.028 317 173 11 78 366 2 275 4.56e-11 65.9
MS.gene27992.t1 AT2G43770 32.164 171 83 8 288 429 46 212 7.24e-11 63.9

Find 122 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTGAACCGTCGTCGTTT+AGG 0.241194 4.4:+11669767 None:intergenic
AGGTGCTGTCGTCAGAGTTT+AGG 0.258964 4.4:+11670538 None:intergenic
CACTGAACCGTCGTCGTTTA+GGG 0.263337 4.4:+11669768 None:intergenic
CTAACGACTTAACCGGTTTC+CGG 0.279074 4.4:+11669524 None:intergenic
GGTGCTGTCGTCAGAGTTTA+GGG 0.283281 4.4:+11670539 None:intergenic
AGGTGATAGGGCGGGAAGAG+TGG 0.294960 4.4:+11670394 None:intergenic
CTGATCGGACGGTTAGGATT+TGG 0.299611 4.4:-11669597 MS.gene27992:CDS
GGGACAAAACACTCAAAATA+TGG 0.300866 4.4:-11669990 MS.gene27992:CDS
GCGATCGTTCGATATTAGTA+TGG 0.311845 4.4:-11669729 MS.gene27992:CDS
AAGGTTTGGCAACTTTCTAT+TGG 0.313481 4.4:-11669409 MS.gene27992:CDS
TGGGTGTGATCTGGCCTCTC+AGG 0.327971 4.4:-11669389 MS.gene27992:CDS
CCGCGTCTTCATGTGCTTTG+AGG 0.338253 4.4:+11669938 None:intergenic
CGCGTCTTCATGTGCTTTGA+GGG 0.347304 4.4:+11669939 None:intergenic
CGGCGTAAAGGAGGTTGTTA+TGG 0.349908 4.4:+11670361 None:intergenic
AACACAGAAACGACGCCGTT+TGG 0.351900 4.4:+11669457 None:intergenic
GGCGGGAAGAGTGGGGTGTG+AGG 0.353577 4.4:+11670403 None:intergenic
GGAAGAGTGGGGTGTGAGGG+AGG 0.357497 4.4:+11670407 None:intergenic
CGGATAAACGAATACGCGTT+TGG 0.358390 4.4:-11669867 MS.gene27992:CDS
TGGTTGTGAGTGGGGCTTTG+AGG 0.360564 4.4:-11669681 MS.gene27992:CDS
ACTTTCTATTGGGTGTGATC+TGG 0.363822 4.4:-11669398 MS.gene27992:CDS
AGGTTTGGCAACTTTCTATT+GGG 0.364284 4.4:-11669408 MS.gene27992:CDS
GCATGTTGTTGTTTCTTAGA+TGG 0.370683 4.4:+11670591 None:intergenic
TTTAGTTGAACCGGAGGAAA+TGG 0.373748 4.4:+11670269 None:intergenic
GCGTTATCTACTCCGTTTCA+TGG 0.381531 4.4:-11670011 MS.gene27992:CDS
GAGTGAGATGGTTGAGTTGT+GGG 0.382726 4.4:+11670617 None:intergenic
GATGGTTGAGTTGTGGGTGG+TGG 0.397246 4.4:+11670623 None:intergenic
GCACGTTCTAGTTGCGACTT+TGG 0.398974 4.4:-11669821 MS.gene27992:CDS
GGTTGTGAGTGGGGCTTTGA+GGG 0.403858 4.4:-11669680 MS.gene27992:CDS
GTGGGTGGTGGTGGCGCAGT+TGG 0.406772 4.4:+11670635 None:intergenic
TGAACCGGAGGAAATGGATT+TGG 0.407021 4.4:+11670275 None:intergenic
TTCTTTAGACGGTGAAATAA+AGG 0.410331 4.4:-11669428 MS.gene27992:CDS
GCGGGAAGAGTGGGGTGTGA+GGG 0.410576 4.4:+11670404 None:intergenic
GTGATAGGGCGGGAAGAGTG+GGG 0.413241 4.4:+11670396 None:intergenic
GACGGTCGTAGACGTTGATT+TGG 0.422093 4.4:+11670328 None:intergenic
GGTGATAGGGCGGGAAGAGT+GGG 0.426722 4.4:+11670395 None:intergenic
GGATAAACGAATACGCGTTT+GGG 0.431453 4.4:-11669866 MS.gene27992:CDS
TAGACGGTGAAATAAAGGTT+TGG 0.432760 4.4:-11669423 MS.gene27992:CDS
GGTGGTGGTGGCGCAGTTGG+CGG 0.433020 4.4:+11670638 None:intergenic
TGGAGGGCAAGGGAGGTGAT+AGG 0.434136 4.4:+11670381 None:intergenic
GGTTGAGTTGTGGGTGGTGG+TGG 0.442279 4.4:+11670626 None:intergenic
TCTCGATGGTCACCGGAAAC+CGG 0.448997 4.4:-11669536 MS.gene27992:CDS
GGTGGTGGCGCAGTTGGCGG+AGG 0.449359 4.4:+11670641 None:intergenic
GGAGATATGTACACGTTTGA+CGG 0.458892 4.4:+11670310 None:intergenic
TGCTGAGTGGTTCTGCTGAT+CGG 0.460135 4.4:-11669612 MS.gene27992:CDS
ATTGCCGCTAACGACTTAAC+CGG 0.460335 4.4:+11669517 None:intergenic
ATTTGGAAGCGGGTTTATGA+TGG 0.461264 4.4:-11669580 MS.gene27992:CDS
GACGGTTAGGATTTGGAAGC+GGG 0.467016 4.4:-11669590 MS.gene27992:CDS
GGGCAAGGGAGGTGATAGGG+CGG 0.468879 4.4:+11670385 None:intergenic
GTTGTTGTTTCTTAGATGGA+TGG 0.471265 4.4:+11670595 None:intergenic
GTTCTGCTGATCGGACGGTT+AGG 0.471712 4.4:-11669603 MS.gene27992:CDS
AACACCAAATCCATTTCCTC+CGG 0.474707 4.4:-11670279 MS.gene27992:CDS
GGAGGTTGTTATGGAGGGCA+AGG 0.476994 4.4:+11670370 None:intergenic
GGCGTCGTTTCTGTGTTTAG+TGG 0.477810 4.4:-11669451 MS.gene27992:CDS
ATCAACTAATCATGAGGTTA+CGG 0.480358 4.4:-11670683 None:intergenic
GTGTTTAGTGGTTCTTTAGA+CGG 0.480377 4.4:-11669439 MS.gene27992:CDS
GTCGCCACGTAAAACGTCTC+TGG 0.481507 4.4:-11670077 MS.gene27992:CDS
GGAGGAGGAAACGGCGGAGA+CGG 0.486240 4.4:+11670121 None:intergenic
AGAGGATAGTGCAAATCATA+TGG 0.498930 4.4:-11669701 MS.gene27992:CDS
GGGTGTGATCTGGCCTCTCA+GGG 0.499450 4.4:-11669388 MS.gene27992:CDS
GTTGTTATGGAGGGCAAGGG+AGG 0.499756 4.4:+11670374 None:intergenic
TGTGGTGGTGGTTCCAGACA+CGG 0.508169 4.4:+11670193 None:intergenic
ACGGACCTTGCCGTCTCCAA+CGG 0.513299 4.4:-11670033 MS.gene27992:CDS
CGTTATCTACTCCGTTTCAT+GGG 0.513874 4.4:-11670010 MS.gene27992:CDS
TTGTGAGTGGGGCTTTGAGG+GGG 0.519148 4.4:-11669678 MS.gene27992:CDS
AAGACTGTAAAATCCGTGTC+TGG 0.524903 4.4:-11670206 MS.gene27992:CDS
GGAGGGCAAGGGAGGTGATA+GGG 0.530623 4.4:+11670382 None:intergenic
GGAAATGGATTTGGTGTTGA+AGG 0.532385 4.4:+11670284 None:intergenic
GTGTGAGGGAGGTGATGCAG+TGG 0.535067 4.4:+11670418 None:intergenic
TGTTGTGTTGGTGTTCTCGA+TGG 0.537133 4.4:-11669550 MS.gene27992:CDS
GGACGGTTAGGATTTGGAAG+CGG 0.537738 4.4:-11669591 MS.gene27992:CDS
ACGGCGGAGACGGTCGTTGA+CGG 0.541326 4.4:+11670131 None:intergenic
GGAGTGAGATGGTTGAGTTG+TGG 0.545632 4.4:+11670616 None:intergenic
CTTAGATGGATGGAGTGAGA+TGG 0.547151 4.4:+11670605 None:intergenic
TGAACATGCCGACGCTGTCA+CGG 0.551139 4.4:-11670052 MS.gene27992:CDS
TTTGGAGAGATGGAACTGAT+GGG 0.554738 4.4:+11670481 None:intergenic
ATCCGATGATCCTGTGTAGA+CGG 0.555899 4.4:+11669885 None:intergenic
GGCAAGGGAGGTGATAGGGC+GGG 0.557100 4.4:+11670386 None:intergenic
TTTCAGTTGCGGCGTAAAGG+AGG 0.557568 4.4:+11670352 None:intergenic
GTTGTGAGTGGGGCTTTGAG+GGG 0.558441 4.4:-11669679 MS.gene27992:CDS
TGGATTTGGTGTTGAAGGTG+TGG 0.559424 4.4:+11670289 None:intergenic
CGTAAAGGAGGTTGTTATGG+AGG 0.563981 4.4:+11670364 None:intergenic
AGGAGACGGAGAAGTTGTTG+AGG 0.565001 4.4:+11670442 None:intergenic
GTAGATAACGCCGTTGGAGA+CGG 0.567014 4.4:+11670023 None:intergenic
TGAGATGGTTGAGTTGTGGG+TGG 0.572126 4.4:+11670620 None:intergenic
GTCGTTGACGGTGGGAAGAG+TGG 0.575255 4.4:+11670143 None:intergenic
CGCGTTTGGGCGAAACCCGT+CGG 0.575820 4.4:-11669853 MS.gene27992:CDS
CGATCGTTCGATATTAGTAT+GGG 0.577039 4.4:-11669728 MS.gene27992:CDS
AACCGTCTACACAGGATCAT+CGG 0.578209 4.4:-11669887 MS.gene27992:CDS
GAGGTTGTTATGGAGGGCAA+GGG 0.579267 4.4:+11670371 None:intergenic
GAAAGTCAAAGTTCTCCAAA+CGG 0.579574 4.4:-11669472 MS.gene27992:CDS
GAAGACCATGTCTTTAGAGA+AGG 0.579938 4.4:+11670239 None:intergenic
GCAAATCATATGGTTGTGAG+TGG 0.588639 4.4:-11669691 MS.gene27992:CDS
GAGTGGTTCTGCTGATCGGA+CGG 0.588641 4.4:-11669608 MS.gene27992:CDS
GAGGGAGGTGATGCAGTGGT+AGG 0.589481 4.4:+11670422 None:intergenic
GTAAAGGAGGTTGTTATGGA+GGG 0.593750 4.4:+11670365 None:intergenic
GTCTCTGATTTGTTGCTGAG+TGG 0.599006 4.4:-11669625 MS.gene27992:CDS
TTGGTGTTCTCGATGGTCAC+CGG 0.602395 4.4:-11669543 MS.gene27992:CDS
CATCGCCTTCTCTAAAGACA+TGG 0.603402 4.4:-11670244 MS.gene27992:CDS
GGTGATGCAGTGGTAGGAGA+CGG 0.605357 4.4:+11670428 None:intergenic
GCGGAGACGGTCGTTGACGG+TGG 0.612720 4.4:+11670134 None:intergenic
CGGAGACGGTCGTTGACGGT+GGG 0.614987 4.4:+11670135 None:intergenic
GGCAAGGTCCGTGACAGCGT+CGG 0.616914 4.4:+11670044 None:intergenic
AACGATGGAACCGTCTACAC+AGG 0.620199 4.4:-11669895 MS.gene27992:CDS
AACGGAGTAGATAACGCCGT+TGG 0.621817 4.4:+11670017 None:intergenic
AAATCATATGGTTGTGAGTG+GGG 0.624059 4.4:-11669689 MS.gene27992:CDS
TAACGCCGTTGGAGACGGCA+AGG 0.624466 4.4:+11670028 None:intergenic
CAGAAAATATTGACCCTGAG+AGG 0.634509 4.4:+11669375 None:intergenic
CATGTCTTTAGAGAAGGCGA+TGG 0.637582 4.4:+11670245 None:intergenic
GCGGTTGCAGTTTCTAACGA+TGG 0.639355 4.4:-11669910 MS.gene27992:CDS
GAAGAGTGGTTAACTTGCGG+TGG 0.646427 4.4:+11670157 None:intergenic
GATATTAGTATGGGAACGAG+AGG 0.652105 4.4:-11669719 MS.gene27992:CDS
AATTCAAGAGTATAATGATG+AGG 0.660610 4.4:-11669497 MS.gene27992:CDS
CAAATCATATGGTTGTGAGT+GGG 0.660806 4.4:-11669690 MS.gene27992:CDS
GAAACCGGTTAAGTCGTTAG+CGG 0.662961 4.4:-11669521 MS.gene27992:CDS
TGGGAAGAGTGGTTAACTTG+CGG 0.663058 4.4:+11670154 None:intergenic
AACTAATCATGAGGTTACGG+TGG 0.663538 4.4:-11670680 None:intergenic
CCTCAAAGCACATGAAGACG+CGG 0.680860 4.4:-11669938 MS.gene27992:CDS
GTAGTTGCTATAGAGAGTTG+AGG 0.682756 4.4:+11670518 None:intergenic
TTTGGAGAAGCATAAATCAG+CGG 0.685092 4.4:-11669803 MS.gene27992:CDS
GCTTTAGCCCTAAACGACGA+CGG 0.704964 4.4:-11669775 MS.gene27992:CDS
ACATGAAGACGCGGTCAACG+CGG 0.728323 4.4:-11669929 MS.gene27992:CDS
TTTGTGGGAGGAGGAAACGG+CGG 0.729879 4.4:+11670115 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAAGAGTATAATGATG+AGG - chr4.4:11670541-11670560 MS.gene27992:CDS 25.0%
TTCTTTAGACGGTGAAATAA+AGG - chr4.4:11670610-11670629 MS.gene27992:CDS 30.0%
AAATCATATGGTTGTGAGTG+GGG - chr4.4:11670349-11670368 MS.gene27992:CDS 35.0%
AAGGTTTGGCAACTTTCTAT+TGG - chr4.4:11670629-11670648 MS.gene27992:CDS 35.0%
AGAGGATAGTGCAAATCATA+TGG - chr4.4:11670337-11670356 MS.gene27992:CDS 35.0%
AGGTTTGGCAACTTTCTATT+GGG - chr4.4:11670630-11670649 MS.gene27992:CDS 35.0%
CAAATCATATGGTTGTGAGT+GGG - chr4.4:11670348-11670367 MS.gene27992:CDS 35.0%
CGATCGTTCGATATTAGTAT+GGG - chr4.4:11670310-11670329 MS.gene27992:CDS 35.0%
GAAAGTCAAAGTTCTCCAAA+CGG - chr4.4:11670566-11670585 MS.gene27992:CDS 35.0%
GGGACAAAACACTCAAAATA+TGG - chr4.4:11670048-11670067 MS.gene27992:CDS 35.0%
GTGTTTAGTGGTTCTTTAGA+CGG - chr4.4:11670599-11670618 MS.gene27992:CDS 35.0%
TAGACGGTGAAATAAAGGTT+TGG - chr4.4:11670615-11670634 MS.gene27992:CDS 35.0%
! TTTGGAGAAGCATAAATCAG+CGG - chr4.4:11670235-11670254 MS.gene27992:CDS 35.0%
!! GCATGTTGTTGTTTCTTAGA+TGG + chr4.4:11669450-11669469 None:intergenic 35.0%
!! GTTGTTGTTTCTTAGATGGA+TGG + chr4.4:11669446-11669465 None:intergenic 35.0%
!!! GGATGGTTATGTAGTTTTGT+GGG + chr4.4:11669941-11669960 None:intergenic 35.0%
!!! GGTTTTGAGAAGAGAGTTTT+TGG + chr4.4:11669578-11669597 None:intergenic 35.0%
!!! GTTGATTTGGTTTTCAGTTG+CGG + chr4.4:11669700-11669719 None:intergenic 35.0%
AACACCAAATCCATTTCCTC+CGG - chr4.4:11669759-11669778 MS.gene27992:CDS 40.0%
AAGACTGTAAAATCCGTGTC+TGG - chr4.4:11669832-11669851 MS.gene27992:CDS 40.0%
ACTTTCTATTGGGTGTGATC+TGG - chr4.4:11670640-11670659 MS.gene27992:CDS 40.0%
CAGAAAATATTGACCCTGAG+AGG + chr4.4:11670666-11670685 None:intergenic 40.0%
CGTTATCTACTCCGTTTCAT+GGG - chr4.4:11670028-11670047 MS.gene27992:CDS 40.0%
GATATTAGTATGGGAACGAG+AGG - chr4.4:11670319-11670338 MS.gene27992:CDS 40.0%
GCAAATCATATGGTTGTGAG+TGG - chr4.4:11670347-11670366 MS.gene27992:CDS 40.0%
GCGATCGTTCGATATTAGTA+TGG - chr4.4:11670309-11670328 MS.gene27992:CDS 40.0%
GGAGATATGTACACGTTTGA+CGG + chr4.4:11669731-11669750 None:intergenic 40.0%
GGATAAACGAATACGCGTTT+GGG - chr4.4:11670172-11670191 MS.gene27992:CDS 40.0%
GTAAAGGAGGTTGTTATGGA+GGG + chr4.4:11669676-11669695 None:intergenic 40.0%
GTAGTTGCTATAGAGAGTTG+AGG + chr4.4:11669523-11669542 None:intergenic 40.0%
TTTAGTTGAACCGGAGGAAA+TGG + chr4.4:11669772-11669791 None:intergenic 40.0%
TTTGGAGAGATGGAACTGAT+GGG + chr4.4:11669560-11669579 None:intergenic 40.0%
! AGACACGGATTTTACAGTCT+TGG + chr4.4:11669833-11669852 None:intergenic 40.0%
! ATTTGGAAGCGGGTTTATGA+TGG - chr4.4:11670458-11670477 MS.gene27992:CDS 40.0%
! GAAGACCATGTCTTTAGAGA+AGG + chr4.4:11669802-11669821 None:intergenic 40.0%
! GAGTGTTTTGTCCCATGAAA+CGG + chr4.4:11670042-11670061 None:intergenic 40.0%
! GATTTTACAGTCTTGGTGAG+TGG + chr4.4:11669826-11669845 None:intergenic 40.0%
!! CGGATGGTTATGTAGTTTTG+TGG + chr4.4:11669942-11669961 None:intergenic 40.0%
!! GAAGAGAGTTTTTGGAGAGA+TGG + chr4.4:11669570-11669589 None:intergenic 40.0%
!! GATGGATCGTTTTGTTGTGT+TGG - chr4.4:11670476-11670495 MS.gene27992:CDS 40.0%
!! GGAAATGGATTTGGTGTTGA+AGG + chr4.4:11669757-11669776 None:intergenic 40.0%
!! TTTTGGAGAGATGGAACTGA+TGG + chr4.4:11669561-11669580 None:intergenic 40.0%
!!! AACGTGCTTTTTTTCTCCGA+CGG + chr4.4:11670204-11670223 None:intergenic 40.0%
!!! GACGGTTCAGTGTTATTTTC+AGG - chr4.4:11670281-11670300 MS.gene27992:CDS 40.0%
!!! GGTGGTTGTTGTTGTTTTTG+TGG + chr4.4:11669866-11669885 None:intergenic 40.0%
!!! GGTTGTTGTTGTTTTTGTGG+TGG + chr4.4:11669863-11669882 None:intergenic 40.0%
!!! TGGTTATGTAGTTTTGTGGG+AGG + chr4.4:11669938-11669957 None:intergenic 40.0%
!!! TGTTGTTGTTTTTGTGGTGG+TGG + chr4.4:11669860-11669879 None:intergenic 40.0%
!!! TTATGTAGTTTTGTGGGAGG+AGG + chr4.4:11669935-11669954 None:intergenic 40.0%
AACCGTCTACACAGGATCAT+CGG - chr4.4:11670151-11670170 MS.gene27992:CDS 45.0%
ATCCGATGATCCTGTGTAGA+CGG + chr4.4:11670156-11670175 None:intergenic 45.0%
ATTGCCGCTAACGACTTAAC+CGG + chr4.4:11670524-11670543 None:intergenic 45.0%
CGGATAAACGAATACGCGTT+TGG - chr4.4:11670171-11670190 MS.gene27992:CDS 45.0%
CGTAAAGGAGGTTGTTATGG+AGG + chr4.4:11669677-11669696 None:intergenic 45.0%
CTTAGATGGATGGAGTGAGA+TGG + chr4.4:11669436-11669455 None:intergenic 45.0%
GAAACCGGTTAAGTCGTTAG+CGG - chr4.4:11670517-11670536 MS.gene27992:CDS 45.0%
GCGTTATCTACTCCGTTTCA+TGG - chr4.4:11670027-11670046 MS.gene27992:CDS 45.0%
! CAATCCAGAGACGTTTTACG+TGG + chr4.4:11669968-11669987 None:intergenic 45.0%
! CATCGCCTTCTCTAAAGACA+TGG - chr4.4:11669794-11669813 MS.gene27992:CDS 45.0%
! CATGTCTTTAGAGAAGGCGA+TGG + chr4.4:11669796-11669815 None:intergenic 45.0%
! CTAACGACTTAACCGGTTTC+CGG + chr4.4:11670517-11670536 None:intergenic 45.0%
! GAGTGAGATGGTTGAGTTGT+GGG + chr4.4:11669424-11669443 None:intergenic 45.0%
! TGAACCGGAGGAAATGGATT+TGG + chr4.4:11669766-11669785 None:intergenic 45.0%
! TGGGAAGAGTGGTTAACTTG+CGG + chr4.4:11669887-11669906 None:intergenic 45.0%
! TGTTGTGTTGGTGTTCTCGA+TGG - chr4.4:11670488-11670507 MS.gene27992:CDS 45.0%
!! AGTTTTGTGGGAGGAGGAAA+CGG + chr4.4:11669929-11669948 None:intergenic 45.0%
!! GTCTCTGATTTGTTGCTGAG+TGG - chr4.4:11670413-11670432 MS.gene27992:CDS 45.0%
!! TGGATTTGGTGTTGAAGGTG+TGG + chr4.4:11669752-11669771 None:intergenic 45.0%
!!! ACGTGCTTTTTTTCTCCGAC+GGG + chr4.4:11670203-11670222 None:intergenic 45.0%
!!! GATGGTTTTAGTTGAACCGG+AGG + chr4.4:11669778-11669797 None:intergenic 45.0%
!!! GGCGATGGTTTTAGTTGAAC+CGG + chr4.4:11669781-11669800 None:intergenic 45.0%
AACACAGAAACGACGCCGTT+TGG + chr4.4:11670584-11670603 None:intergenic 50.0%
AACGATGGAACCGTCTACAC+AGG - chr4.4:11670143-11670162 MS.gene27992:CDS 50.0%
AACGGAGTAGATAACGCCGT+TGG + chr4.4:11670024-11670043 None:intergenic 50.0%
ACACTGAACCGTCGTCGTTT+AGG + chr4.4:11670274-11670293 None:intergenic 50.0%
AGGAGACGGAGAAGTTGTTG+AGG + chr4.4:11669599-11669618 None:intergenic 50.0%
CACTGAACCGTCGTCGTTTA+GGG + chr4.4:11670273-11670292 None:intergenic 50.0%
CCTCAAAGCACATGAAGACG+CGG - chr4.4:11670100-11670119 MS.gene27992:CDS 50.0%
CGCGTCTTCATGTGCTTTGA+GGG + chr4.4:11670102-11670121 None:intergenic 50.0%
CGGCGTAAAGGAGGTTGTTA+TGG + chr4.4:11669680-11669699 None:intergenic 50.0%
CTGATCGGACGGTTAGGATT+TGG - chr4.4:11670441-11670460 MS.gene27992:CDS 50.0%
GACGGTTAGGATTTGGAAGC+GGG - chr4.4:11670448-11670467 MS.gene27992:CDS 50.0%
GAGGTTGTTATGGAGGGCAA+GGG + chr4.4:11669670-11669689 None:intergenic 50.0%
GCGGTTGCAGTTTCTAACGA+TGG - chr4.4:11670128-11670147 MS.gene27992:CDS 50.0%
GCTTTAGCCCTAAACGACGA+CGG - chr4.4:11670263-11670282 MS.gene27992:CDS 50.0%
GGACGGTTAGGATTTGGAAG+CGG - chr4.4:11670447-11670466 MS.gene27992:CDS 50.0%
GGCGTCGTTTCTGTGTTTAG+TGG - chr4.4:11670587-11670606 MS.gene27992:CDS 50.0%
GTAGATAACGCCGTTGGAGA+CGG + chr4.4:11670018-11670037 None:intergenic 50.0%
TTTCAGTTGCGGCGTAAAGG+AGG + chr4.4:11669689-11669708 None:intergenic 50.0%
! AGAGACGTTTTACGTGGCGA+CGG + chr4.4:11669962-11669981 None:intergenic 50.0%
! AGGTGCTGTCGTCAGAGTTT+AGG + chr4.4:11669503-11669522 None:intergenic 50.0%
! GACGGTCGTAGACGTTGATT+TGG + chr4.4:11669713-11669732 None:intergenic 50.0%
! GCACGTTCTAGTTGCGACTT+TGG - chr4.4:11670217-11670236 MS.gene27992:CDS 50.0%
! GGAGTGAGATGGTTGAGTTG+TGG + chr4.4:11669425-11669444 None:intergenic 50.0%
! GGTGCTGTCGTCAGAGTTTA+GGG + chr4.4:11669502-11669521 None:intergenic 50.0%
! GGTTTTCAGTTGCGGCGTAA+AGG + chr4.4:11669692-11669711 None:intergenic 50.0%
! TGAGATGGTTGAGTTGTGGG+TGG + chr4.4:11669421-11669440 None:intergenic 50.0%
! TGCTGAGTGGTTCTGCTGAT+CGG - chr4.4:11670426-11670445 MS.gene27992:CDS 50.0%
! TTGGTGTTCTCGATGGTCAC+CGG - chr4.4:11670495-11670514 MS.gene27992:CDS 50.0%
!! GAAGAGTGGTTAACTTGCGG+TGG + chr4.4:11669884-11669903 None:intergenic 50.0%
ACATGAAGACGCGGTCAACG+CGG - chr4.4:11670109-11670128 MS.gene27992:CDS 55.0%
CCGCGTCTTCATGTGCTTTG+AGG + chr4.4:11670103-11670122 None:intergenic 55.0%
GGAGGTTGTTATGGAGGGCA+AGG + chr4.4:11669671-11669690 None:intergenic 55.0%
GGTGATGCAGTGGTAGGAGA+CGG + chr4.4:11669613-11669632 None:intergenic 55.0%
GTCGCCACGTAAAACGTCTC+TGG - chr4.4:11669961-11669980 MS.gene27992:CDS 55.0%
GTTCTGCTGATCGGACGGTT+AGG - chr4.4:11670435-11670454 MS.gene27992:CDS 55.0%
GTTGTTATGGAGGGCAAGGG+AGG + chr4.4:11669667-11669686 None:intergenic 55.0%
TCTCGATGGTCACCGGAAAC+CGG - chr4.4:11670502-11670521 MS.gene27992:CDS 55.0%
TGAACATGCCGACGCTGTCA+CGG - chr4.4:11669986-11670005 MS.gene27992:CDS 55.0%
TGGTTGTGAGTGGGGCTTTG+AGG - chr4.4:11670357-11670376 MS.gene27992:CDS 55.0%
TTTGTGGGAGGAGGAAACGG+CGG + chr4.4:11669926-11669945 None:intergenic 55.0%
! ACGTTTTACGTGGCGACGGA+TGG + chr4.4:11669958-11669977 None:intergenic 55.0%
! GAGTGGTTCTGCTGATCGGA+CGG - chr4.4:11670430-11670449 MS.gene27992:CDS 55.0%
! GATGGTTGAGTTGTGGGTGG+TGG + chr4.4:11669418-11669437 None:intergenic 55.0%
! GGTTGTGAGTGGGGCTTTGA+GGG - chr4.4:11670358-11670377 MS.gene27992:CDS 55.0%
!! GTTGTGAGTGGGGCTTTGAG+GGG - chr4.4:11670359-11670378 MS.gene27992:CDS 55.0%
!! TGTGGTGGTGGTTCCAGACA+CGG + chr4.4:11669848-11669867 None:intergenic 55.0%
!! TTGTGAGTGGGGCTTTGAGG+GGG - chr4.4:11670360-11670379 MS.gene27992:CDS 55.0%
ACGGACCTTGCCGTCTCCAA+CGG - chr4.4:11670005-11670024 MS.gene27992:CDS 60.0%
AGGTGATAGGGCGGGAAGAG+TGG + chr4.4:11669647-11669666 None:intergenic 60.0%
GAGGGAGGTGATGCAGTGGT+AGG + chr4.4:11669619-11669638 None:intergenic 60.0%
GGAGGGCAAGGGAGGTGATA+GGG + chr4.4:11669659-11669678 None:intergenic 60.0%
GGGTGTGATCTGGCCTCTCA+GGG - chr4.4:11670650-11670669 MS.gene27992:CDS 60.0%
GGTGATAGGGCGGGAAGAGT+GGG + chr4.4:11669646-11669665 None:intergenic 60.0%
GTCGTTGACGGTGGGAAGAG+TGG + chr4.4:11669898-11669917 None:intergenic 60.0%
GTGATAGGGCGGGAAGAGTG+GGG + chr4.4:11669645-11669664 None:intergenic 60.0%
GTGTGAGGGAGGTGATGCAG+TGG + chr4.4:11669623-11669642 None:intergenic 60.0%
TAACGCCGTTGGAGACGGCA+AGG + chr4.4:11670013-11670032 None:intergenic 60.0%
TGGAGGGCAAGGGAGGTGAT+AGG + chr4.4:11669660-11669679 None:intergenic 60.0%
TGGGTGTGATCTGGCCTCTC+AGG - chr4.4:11670649-11670668 MS.gene27992:CDS 60.0%
! GGTTGAGTTGTGGGTGGTGG+TGG + chr4.4:11669415-11669434 None:intergenic 60.0%
ACGGCGGAGACGGTCGTTGA+CGG + chr4.4:11669910-11669929 None:intergenic 65.0%
CGCGTTTGGGCGAAACCCGT+CGG - chr4.4:11670185-11670204 MS.gene27992:CDS 65.0%
CGGAGACGGTCGTTGACGGT+GGG + chr4.4:11669906-11669925 None:intergenic 65.0%
GCGGGAAGAGTGGGGTGTGA+GGG + chr4.4:11669637-11669656 None:intergenic 65.0%
GGAAGAGTGGGGTGTGAGGG+AGG + chr4.4:11669634-11669653 None:intergenic 65.0%
GGAGGAGGAAACGGCGGAGA+CGG + chr4.4:11669920-11669939 None:intergenic 65.0%
GGCAAGGGAGGTGATAGGGC+GGG + chr4.4:11669655-11669674 None:intergenic 65.0%
GGCAAGGTCCGTGACAGCGT+CGG + chr4.4:11669997-11670016 None:intergenic 65.0%
GGGCAAGGGAGGTGATAGGG+CGG + chr4.4:11669656-11669675 None:intergenic 65.0%
GCGGAGACGGTCGTTGACGG+TGG + chr4.4:11669907-11669926 None:intergenic 70.0%
GGCGGGAAGAGTGGGGTGTG+AGG + chr4.4:11669638-11669657 None:intergenic 70.0%
!! GGTGGTGGTGGCGCAGTTGG+CGG + chr4.4:11669403-11669422 None:intergenic 70.0%
!! GTGGGTGGTGGTGGCGCAGT+TGG + chr4.4:11669406-11669425 None:intergenic 70.0%
! GGTGGTGGCGCAGTTGGCGG+AGG + chr4.4:11669400-11669419 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 11669366 11670694 11669366 ID=MS.gene27992
chr4.4 mRNA 11669366 11670694 11669366 ID=MS.gene27992.t1;Parent=MS.gene27992
chr4.4 exon 11669366 11670694 11669366 ID=MS.gene27992.t1.exon1;Parent=MS.gene27992.t1
chr4.4 CDS 11669366 11670694 11669366 ID=cds.MS.gene27992.t1;Parent=MS.gene27992.t1
Gene Sequence

>MS.gene27992

ATGAGGTTACGGTGGTTGTTAGACACGTGCTCCTCCGCCAACTGCGCCACCACCACCCACAACTCAACCATCTCACTCCATCCATCTAAGAAACAACAACATGCATCTGACTCAAGCACTTCTTCTTCTTCAACCCTAAACTCTGACGACAGCACCTCAACTCTCTATAGCAACTACTCAATGCAAACACTCCCATCAGTTCCATCTCTCCAAAAACTCTCTTCTCAAAACCTCAACAACTTCTCCGTCTCCTACCACTGCATCACCTCCCTCACACCCCACTCTTCCCGCCCTATCACCTCCCTTGCCCTCCATAACAACCTCCTTTACGCCGCAACTGAAAACCAAATCAACGTCTACGACCGTCAAACGTGTACATATCTCCACACCTTCAACACCAAATCCATTTCCTCCGGTTCAACTAAAACCATCGCCTTCTCTAAAGACATGGTCTTCACCACTCACCAAGACTGTAAAATCCGTGTCTGGAACCACCACCACAAAAACAACAACAACCACCGCAAGTTAACCACTCTTCCCACCGTCAACGACCGTCTCCGCCGTTTCCTCCTCCCACAAAACTACATAACCATCCGTCGCCACGTAAAACGTCTCTGGATTGAACATGCCGACGCTGTCACGGACCTTGCCGTCTCCAACGGCGTTATCTACTCCGTTTCATGGGACAAAACACTCAAAATATGGAGAATCTCTGATCTTCGTTGCTTAGAATCCCTCAAAGCACATGAAGACGCGGTCAACGCGGTTGCAGTTTCTAACGATGGAACCGTCTACACAGGATCATCGGATAAACGAATACGCGTTTGGGCGAAACCCGTCGGAGAAAAAAAGCACGTTCTAGTTGCGACTTTGGAGAAGCATAAATCAGCGGTTAATGCTTTAGCCCTAAACGACGACGGTTCAGTGTTATTTTCAGGTGCATGCGATCGTTCGATATTAGTATGGGAACGAGAGGATAGTGCAAATCATATGGTTGTGAGTGGGGCTTTGAGGGGGCATCAGAAAGCAATACTGTGTTTGATCAACGTCTCTGATTTGTTGCTGAGTGGTTCTGCTGATCGGACGGTTAGGATTTGGAAGCGGGTTTATGATGGATCGTTTTGTTGTGTTGGTGTTCTCGATGGTCACCGGAAACCGGTTAAGTCGTTAGCGGCAATTCAAGAGTATAATGATGAGGATGAAAGTCAAAGTTCTCCAAACGGCGTCGTTTCTGTGTTTAGTGGTTCTTTAGACGGTGAAATAAAGGTTTGGCAACTTTCTATTGGGTGTGATCTGGCCTCTCAGGGTCAATATTTTCTGAATTCCTAG
Protein sequence

>MS.gene27992.t1

MRLRWLLDTCSSANCATTTHNSTISLHPSKKQQHASDSSTSSSSTLNSDDSTSTLYSNYSMQTLPSVPSLQKLSSQNLNNFSVSYHCITSLTPHSSRPITSLALHNNLLYAATENQINVYDRQTCTYLHTFNTKSISSGSTKTIAFSKDMVFTTHQDCKIRVWNHHHKNNNNHRKLTTLPTVNDRLRRFLLPQNYITIRRHVKRLWIEHADAVTDLAVSNGVIYSVSWDKTLKIWRISDLRCLESLKAHEDAVNAVAVSNDGTVYTGSSDKRIRVWAKPVGEKKHVLVATLEKHKSAVNALALNDDGSVLFSGACDRSILVWEREDSANHMVVSGALRGHQKAILCLINVSDLLLSGSADRTVRIWKRVYDGSFCCVGVLDGHRKPVKSLAAIQEYNDEDESQSSPNGVVSVFSGSLDGEIKVWQLSIGCDLASQGQYFLNS