Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058907.t1 | XP_013446711.1 | 96.4 | 338 | 10 | 2 | 1 | 336 | 1 | 338 | 1.30E-128 | 469.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058907.t1 | O48716 | 34.4 | 195 | 118 | 4 | 99 | 288 | 99 | 288 | 1.2e-24 | 115.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058907.t1 | A0A072TV41 | 96.4 | 338 | 10 | 2 | 1 | 336 | 1 | 338 | 9.4e-129 | 469.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058907 | MS.gene27992 | 0.827104 | 1.88E-54 | -1.69E-46 |
| MS.gene058907 | MS.gene47956 | 0.965661 | 6.79E-125 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015737 | MS.gene058907 | PPI |
| MS.gene59431 | MS.gene058907 | PPI |
| MS.gene020660 | MS.gene058907 | PPI |
| MS.gene029810 | MS.gene058907 | PPI |
| MS.gene007411 | MS.gene058907 | PPI |
| MS.gene064484 | MS.gene058907 | PPI |
| MS.gene05932 | MS.gene058907 | PPI |
| MS.gene22414 | MS.gene058907 | PPI |
| MS.gene04775 | MS.gene058907 | PPI |
| MS.gene010581 | MS.gene058907 | PPI |
| MS.gene060919 | MS.gene058907 | PPI |
| MS.gene016020 | MS.gene058907 | PPI |
| MS.gene64726 | MS.gene058907 | PPI |
| MS.gene049135 | MS.gene058907 | PPI |
| MS.gene33168 | MS.gene058907 | PPI |
| MS.gene007535 | MS.gene058907 | PPI |
| MS.gene055900 | MS.gene058907 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058907.t1 | MTR_8g089650 | 97.929 | 338 | 5 | 2 | 1 | 336 | 1 | 338 | 0.0 | 667 |
| MS.gene058907.t1 | MTR_4g104410 | 52.778 | 252 | 104 | 8 | 88 | 333 | 33 | 275 | 1.24e-75 | 237 |
| MS.gene058907.t1 | MTR_5g035980 | 47.751 | 289 | 116 | 11 | 67 | 327 | 48 | 329 | 1.99e-74 | 237 |
| MS.gene058907.t1 | MTR_4g106480 | 46.405 | 306 | 137 | 11 | 51 | 341 | 20 | 313 | 5.82e-73 | 231 |
| MS.gene058907.t1 | MTR_3g078380 | 46.000 | 300 | 132 | 11 | 60 | 336 | 30 | 322 | 3.00e-69 | 223 |
| MS.gene058907.t1 | MTR_1g021590 | 41.313 | 259 | 133 | 6 | 99 | 343 | 112 | 365 | 1.69e-57 | 193 |
| MS.gene058907.t1 | MTR_2g060070 | 41.224 | 245 | 136 | 6 | 82 | 324 | 51 | 289 | 3.16e-56 | 189 |
| MS.gene058907.t1 | MTR_5g018520 | 40.506 | 237 | 131 | 4 | 99 | 330 | 100 | 331 | 3.51e-54 | 184 |
| MS.gene058907.t1 | MTR_4g068457 | 37.548 | 261 | 148 | 5 | 79 | 329 | 108 | 363 | 5.90e-52 | 179 |
| MS.gene058907.t1 | MTR_2g101410 | 30.797 | 276 | 163 | 9 | 77 | 341 | 66 | 324 | 3.19e-33 | 128 |
| MS.gene058907.t1 | MTR_3g110185 | 36.000 | 250 | 138 | 9 | 85 | 325 | 78 | 314 | 2.58e-32 | 128 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058907.t1 | AT1G24530 | 64.493 | 276 | 92 | 3 | 62 | 336 | 51 | 321 | 3.49e-127 | 371 |
| MS.gene058907.t1 | AT1G24130 | 45.455 | 286 | 133 | 10 | 67 | 336 | 45 | 323 | 2.48e-72 | 230 |
| MS.gene058907.t1 | AT5G50120 | 42.125 | 273 | 135 | 7 | 85 | 334 | 27 | 299 | 1.48e-65 | 212 |
| MS.gene058907.t1 | AT2G26490 | 40.928 | 237 | 130 | 4 | 99 | 330 | 99 | 330 | 1.72e-56 | 191 |
| MS.gene058907.t1 | AT3G51930 | 39.768 | 259 | 132 | 8 | 84 | 326 | 50 | 300 | 2.48e-54 | 184 |
| MS.gene058907.t1 | AT1G49450 | 41.176 | 238 | 129 | 5 | 99 | 330 | 137 | 369 | 9.99e-51 | 176 |
| MS.gene058907.t1 | AT3G50390 | 39.431 | 246 | 135 | 6 | 99 | 335 | 101 | 341 | 9.10e-50 | 173 |
| MS.gene058907.t1 | AT1G47610 | 41.453 | 234 | 123 | 7 | 99 | 326 | 27 | 252 | 3.55e-48 | 166 |
| MS.gene058907.t1 | AT1G47610 | 25.623 | 281 | 148 | 11 | 66 | 322 | 103 | 346 | 5.13e-11 | 63.5 |
| MS.gene058907.t1 | AT3G18950 | 38.075 | 239 | 135 | 6 | 99 | 330 | 141 | 373 | 1.62e-46 | 164 |
| MS.gene058907.t1 | AT4G34380 | 36.957 | 230 | 137 | 4 | 99 | 324 | 128 | 353 | 1.52e-43 | 157 |
| MS.gene058907.t1 | AT5G16750 | 23.293 | 249 | 149 | 6 | 85 | 326 | 10 | 223 | 1.70e-11 | 65.9 |
Find 0 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGATCGTTCGATATTAGTAT+GGG | + | 57395:17770-17789 | MS.gene058907:CDS | 35.0% | |
| GGGACAAAACACTCAAAATA+TGG | + | 57395:17508-17527 | MS.gene058907:CDS | 35.0% | |
| ! | TTTGGAGAAGCATAAATCAG+CGG | + | 57395:17695-17714 | MS.gene058907:CDS | 35.0% |
| !! | GCATGTTGTTGTTTCTTAGA+TGG | - | 57395:16910-16929 | None:intergenic | 35.0% |
| !! | GTTGTTGTTTCTTAGATGGA+TGG | - | 57395:16906-16925 | None:intergenic | 35.0% |
| !!! | GGATGGTTATGTAGTTTTGT+GGG | - | 57395:17401-17420 | None:intergenic | 35.0% |
| !!! | GGTTTTGAGAAGAGAGTTTT+TGG | - | 57395:17038-17057 | None:intergenic | 35.0% |
| !!! | GTTGATTTGGTTTTCAGTTG+CGG | - | 57395:17160-17179 | None:intergenic | 35.0% |
| AACACCAAATCCATTTCCTC+CGG | + | 57395:17219-17238 | MS.gene058907:CDS | 40.0% | |
| AGAGGATAGTGCGAATCATA+TGG | + | 57395:17797-17816 | MS.gene058907:CDS | 40.0% | |
| CGAATCATATGGTTGTGAGT+GGG | + | 57395:17808-17827 | MS.gene058907:CDS | 40.0% | |
| CGTTATCTACTCCGTTTCAT+GGG | + | 57395:17488-17507 | MS.gene058907:CDS | 40.0% | |
| GAATCATATGGTTGTGAGTG+GGG | + | 57395:17809-17828 | MS.gene058907:CDS | 40.0% | |
| GATATTAGTATGGGAACGAG+AGG | + | 57395:17779-17798 | MS.gene058907:CDS | 40.0% | |
| GCGATCGTTCGATATTAGTA+TGG | + | 57395:17769-17788 | MS.gene058907:CDS | 40.0% | |
| GGAGATATGTACACGTTTGA+CGG | - | 57395:17191-17210 | None:intergenic | 40.0% | |
| GGATAAACGAATACGCGTTT+GGG | + | 57395:17632-17651 | MS.gene058907:CDS | 40.0% | |
| GTAAAGGAGGTTGTTATGGA+GGG | - | 57395:17136-17155 | None:intergenic | 40.0% | |
| GTAGTTGCTATAGAGAGTTG+AGG | - | 57395:16983-17002 | None:intergenic | 40.0% | |
| TTTAGTTGAACCGGAGGAAA+TGG | - | 57395:17232-17251 | None:intergenic | 40.0% | |
| TTTGGAGAGATGGAACTGAT+GGG | - | 57395:17020-17039 | None:intergenic | 40.0% | |
| ! | GAAGACCATGTCTTTAGAGA+AGG | - | 57395:17262-17281 | None:intergenic | 40.0% |
| ! | GAGTGTTTTGTCCCATGAAA+CGG | - | 57395:17502-17521 | None:intergenic | 40.0% |
| !! | CGGATGGTTATGTAGTTTTG+TGG | - | 57395:17402-17421 | None:intergenic | 40.0% |
| !! | GAAGAGAGTTTTTGGAGAGA+TGG | - | 57395:17030-17049 | None:intergenic | 40.0% |
| !! | GGAAATGGATTTGGTGTTGA+AGG | - | 57395:17217-17236 | None:intergenic | 40.0% |
| !! | TTTTGGAGAGATGGAACTGA+TGG | - | 57395:17021-17040 | None:intergenic | 40.0% |
| !!! | AACGTGCTTTTTTTCTCCGA+CGG | - | 57395:17664-17683 | None:intergenic | 40.0% |
| !!! | GACGGTTCAGTGTTATTTTC+AGG | + | 57395:17741-17760 | MS.gene058907:CDS | 40.0% |
| !!! | GGTGGTTGTTGTTGTTTTTG+TGG | - | 57395:17326-17345 | None:intergenic | 40.0% |
| !!! | GGTTGTTGTTGTTTTTGTGG+TGG | - | 57395:17323-17342 | None:intergenic | 40.0% |
| !!! | TGGTTATGTAGTTTTGTGGG+AGG | - | 57395:17398-17417 | None:intergenic | 40.0% |
| !!! | TGTTGTTGTTTTTGTGGTGG+TGG | - | 57395:17320-17339 | None:intergenic | 40.0% |
| !!! | TTATGTAGTTTTGTGGGAGG+AGG | - | 57395:17395-17414 | None:intergenic | 40.0% |
| AACCGTCTACACAGGATCAT+CGG | + | 57395:17611-17630 | MS.gene058907:CDS | 45.0% | |
| AAGACTGCAAAATCCGTGTC+TGG | + | 57395:17292-17311 | MS.gene058907:CDS | 45.0% | |
| ATCCGATGATCCTGTGTAGA+CGG | - | 57395:17616-17635 | None:intergenic | 45.0% | |
| CGGATAAACGAATACGCGTT+TGG | + | 57395:17631-17650 | MS.gene058907:CDS | 45.0% | |
| CGTAAAGGAGGTTGTTATGG+AGG | - | 57395:17137-17156 | None:intergenic | 45.0% | |
| CTTAGATGGATGGAGTGAGA+TGG | - | 57395:16896-16915 | None:intergenic | 45.0% | |
| GCGAATCATATGGTTGTGAG+TGG | + | 57395:17807-17826 | MS.gene058907:CDS | 45.0% | |
| GCGTTATCTACTCCGTTTCA+TGG | + | 57395:17487-17506 | MS.gene058907:CDS | 45.0% | |
| TCGTTGCTTAGAATCCCTCA+AGG | + | 57395:17545-17564 | MS.gene058907:CDS | 45.0% | |
| ! | AGACACGGATTTTGCAGTCT+TGG | - | 57395:17293-17312 | None:intergenic | 45.0% |
| ! | CAATCCAGAGACGTTTTACG+TGG | - | 57395:17428-17447 | None:intergenic | 45.0% |
| ! | CATCGCCTTCTCTAAAGACA+TGG | + | 57395:17254-17273 | MS.gene058907:CDS | 45.0% |
| ! | CATGTCTTTAGAGAAGGCGA+TGG | - | 57395:17256-17275 | None:intergenic | 45.0% |
| ! | GAGTGAGATGGTTGAGTTGT+GGG | - | 57395:16884-16903 | None:intergenic | 45.0% |
| ! | GATTTTGCAGTCTTGGTGAG+TGG | - | 57395:17286-17305 | None:intergenic | 45.0% |
| ! | TGAACCGGAGGAAATGGATT+TGG | - | 57395:17226-17245 | None:intergenic | 45.0% |
| ! | TGGGAAGAGTGGTTAACTTG+CGG | - | 57395:17347-17366 | None:intergenic | 45.0% |
| !! | AGTTTTGTGGGAGGAGGAAA+CGG | - | 57395:17389-17408 | None:intergenic | 45.0% |
| !! | TGGATTTGGTGTTGAAGGTG+TGG | - | 57395:17212-17231 | None:intergenic | 45.0% |
| !!! | ACGTGCTTTTTTTCTCCGAC+GGG | - | 57395:17663-17682 | None:intergenic | 45.0% |
| !!! | GATGGTTTTAGTTGAACCGG+AGG | - | 57395:17238-17257 | None:intergenic | 45.0% |
| !!! | GGCGATGGTTTTAGTTGAAC+CGG | - | 57395:17241-17260 | None:intergenic | 45.0% |
| AACGATGGAACCGTCTACAC+AGG | + | 57395:17603-17622 | MS.gene058907:CDS | 50.0% | |
| AACGGAGTAGATAACGCCGT+TGG | - | 57395:17484-17503 | None:intergenic | 50.0% | |
| ACACTGAACCGTCGTCGTTT+AGG | - | 57395:17734-17753 | None:intergenic | 50.0% | |
| AGGAGACGGAGAAGTTGTTG+AGG | - | 57395:17059-17078 | None:intergenic | 50.0% | |
| CACTGAACCGTCGTCGTTTA+GGG | - | 57395:17733-17752 | None:intergenic | 50.0% | |
| CGGCGTAAAGGAGGTTGTTA+TGG | - | 57395:17140-17159 | None:intergenic | 50.0% | |
| GAGGTTGTTATGGAGGGCAA+GGG | - | 57395:17130-17149 | None:intergenic | 50.0% | |
| GCGGTTGCAGTTTCTAACGA+TGG | + | 57395:17588-17607 | MS.gene058907:CDS | 50.0% | |
| GCTTTAGCCCTAAACGACGA+CGG | + | 57395:17723-17742 | MS.gene058907:CDS | 50.0% | |
| GTAGATAACGCCGTTGGAGA+CGG | - | 57395:17478-17497 | None:intergenic | 50.0% | |
| TTTCAGTTGCGGCGTAAAGG+AGG | - | 57395:17149-17168 | None:intergenic | 50.0% | |
| ! | AGAGACGTTTTACGTGGCGA+CGG | - | 57395:17422-17441 | None:intergenic | 50.0% |
| ! | AGGTGCTGTCGTCAGAGTTT+AGG | - | 57395:16963-16982 | None:intergenic | 50.0% |
| ! | GACGGTCGTAGACGTTGATT+TGG | - | 57395:17173-17192 | None:intergenic | 50.0% |
| ! | GCACGTTCTAGTTGCGACTT+TGG | + | 57395:17677-17696 | MS.gene058907:CDS | 50.0% |
| ! | GGAGTGAGATGGTTGAGTTG+TGG | - | 57395:16885-16904 | None:intergenic | 50.0% |
| ! | GGTGCTGTCGTCAGAGTTTA+GGG | - | 57395:16962-16981 | None:intergenic | 50.0% |
| ! | GGTTTTCAGTTGCGGCGTAA+AGG | - | 57395:17152-17171 | None:intergenic | 50.0% |
| ! | TGAGATGGTTGAGTTGTGGG+TGG | - | 57395:16881-16900 | None:intergenic | 50.0% |
| !! | GAAGAGTGGTTAACTTGCGG+TGG | - | 57395:17344-17363 | None:intergenic | 50.0% |
| ACACGAAGACGCAGTCAACG+CGG | + | 57395:17569-17588 | MS.gene058907:CDS | 55.0% | |
| GGAGGTTGTTATGGAGGGCA+AGG | - | 57395:17131-17150 | None:intergenic | 55.0% | |
| GGTGATGCAGTGGTAGGAGA+CGG | - | 57395:17073-17092 | None:intergenic | 55.0% | |
| GTCGCCACGTAAAACGTCTC+TGG | + | 57395:17421-17440 | MS.gene058907:CDS | 55.0% | |
| GTTGTTATGGAGGGCAAGGG+AGG | - | 57395:17127-17146 | None:intergenic | 55.0% | |
| TGAACATGCCGACGCTGTCA+CGG | + | 57395:17446-17465 | MS.gene058907:CDS | 55.0% | |
| TGCGTCTTCGTGTGCCTTGA+GGG | - | 57395:17562-17581 | None:intergenic | 55.0% | |
| TTTGTGGGAGGAGGAAACGG+CGG | - | 57395:17386-17405 | None:intergenic | 55.0% | |
| ! | ACGTTTTACGTGGCGACGGA+TGG | - | 57395:17418-17437 | None:intergenic | 55.0% |
| ! | GATGGTTGAGTTGTGGGTGG+TGG | - | 57395:16878-16897 | None:intergenic | 55.0% |
| !! | TGTGGTGGTGGTTCCAGACA+CGG | - | 57395:17308-17327 | None:intergenic | 55.0% |
| ACGGACCTTGCCGTCTCCAA+CGG | + | 57395:17465-17484 | MS.gene058907:CDS | 60.0% | |
| AGGTGATAGGGCGGGAAGAG+TGG | - | 57395:17107-17126 | None:intergenic | 60.0% | |
| CTGCGTCTTCGTGTGCCTTG+AGG | - | 57395:17563-17582 | None:intergenic | 60.0% | |
| GAGGGAGGTGATGCAGTGGT+AGG | - | 57395:17079-17098 | None:intergenic | 60.0% | |
| GGAGGGCAAGGGAGGTGATA+GGG | - | 57395:17119-17138 | None:intergenic | 60.0% | |
| GGTGATAGGGCGGGAAGAGT+GGG | - | 57395:17106-17125 | None:intergenic | 60.0% | |
| GTCGTTGACGGTGGGAAGAG+TGG | - | 57395:17358-17377 | None:intergenic | 60.0% | |
| GTGATAGGGCGGGAAGAGTG+GGG | - | 57395:17105-17124 | None:intergenic | 60.0% | |
| GTGTGAGGGAGGTGATGCAG+TGG | - | 57395:17083-17102 | None:intergenic | 60.0% | |
| TAACGCCGTTGGAGACGGCA+AGG | - | 57395:17473-17492 | None:intergenic | 60.0% | |
| TGGAGGGCAAGGGAGGTGAT+AGG | - | 57395:17120-17139 | None:intergenic | 60.0% | |
| ! | GGTTGAGTTGTGGGTGGTGG+TGG | - | 57395:16875-16894 | None:intergenic | 60.0% |
| ACGGCGGAGACGGTCGTTGA+CGG | - | 57395:17370-17389 | None:intergenic | 65.0% | |
| CGCGTTTGGGCGAAACCCGT+CGG | + | 57395:17645-17664 | MS.gene058907:CDS | 65.0% | |
| CGGAGACGGTCGTTGACGGT+GGG | - | 57395:17366-17385 | None:intergenic | 65.0% | |
| GCGGGAAGAGTGGGGTGTGA+GGG | - | 57395:17097-17116 | None:intergenic | 65.0% | |
| GGAAGAGTGGGGTGTGAGGG+AGG | - | 57395:17094-17113 | None:intergenic | 65.0% | |
| GGAGGAGGAAACGGCGGAGA+CGG | - | 57395:17380-17399 | None:intergenic | 65.0% | |
| GGCAAGGGAGGTGATAGGGC+GGG | - | 57395:17115-17134 | None:intergenic | 65.0% | |
| GGCAAGGTCCGTGACAGCGT+CGG | - | 57395:17457-17476 | None:intergenic | 65.0% | |
| GGGCAAGGGAGGTGATAGGG+CGG | - | 57395:17116-17135 | None:intergenic | 65.0% | |
| GCGGAGACGGTCGTTGACGG+TGG | - | 57395:17367-17386 | None:intergenic | 70.0% | |
| GGCGGGAAGAGTGGGGTGTG+AGG | - | 57395:17098-17117 | None:intergenic | 70.0% | |
| !! | GGTGGTGGTGGCGCAGTTGG+CGG | - | 57395:16863-16882 | None:intergenic | 70.0% |
| !! | GTGGGTGGTGGTGGCGCAGT+TGG | - | 57395:16866-16885 | None:intergenic | 70.0% |
| ! | GGTGGTGGCGCAGTTGGCGG+AGG | - | 57395:16860-16879 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57395 | gene | 16826 | 17869 | 16826 | ID=MS.gene058907 |
| 57395 | mRNA | 16826 | 17869 | 16826 | ID=MS.gene058907.t1;Parent=MS.gene058907 |
| 57395 | exon | 16826 | 17869 | 16826 | ID=MS.gene058907.t1.exon1;Parent=MS.gene058907.t1 |
| 57395 | CDS | 16826 | 17869 | 16826 | ID=cds.MS.gene058907.t1;Parent=MS.gene058907.t1 |
| Gene Sequence |
| Protein sequence |
