AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28813

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28813.t1 MTR_7g109550 96.330 218 3 1 1 218 108 320 7.05e-157 443
MS.gene28813.t1 MTR_7g094750 57.923 183 75 1 34 214 90 272 1.82e-72 223
MS.gene28813.t1 MTR_7g094750 57.923 183 75 1 34 214 90 272 3.05e-71 224
MS.gene28813.t1 MTR_2g022560 54.945 182 80 1 35 214 97 278 1.86e-68 217
MS.gene28813.t1 MTR_4g126950 53.191 188 87 1 32 218 106 293 5.89e-68 216
MS.gene28813.t1 MTR_5g033800 47.980 198 103 0 20 217 130 327 5.40e-67 211
MS.gene28813.t1 MTR_8g069790 46.429 224 112 2 2 217 137 360 9.58e-67 214
MS.gene28813.t1 MTR_5g033800 47.980 198 103 0 20 217 130 327 1.43e-65 211
MS.gene28813.t1 MTR_5g059400 48.649 185 92 2 35 217 175 358 2.42e-64 208
MS.gene28813.t1 MTR_1g116130 48.649 185 92 2 35 217 175 358 2.42e-64 208
MS.gene28813.t1 MTR_0136s0010 48.454 194 99 1 25 217 160 353 2.26e-63 206
MS.gene28813.t1 MTR_7g093450 48.980 196 97 2 24 217 237 431 5.30e-63 206
MS.gene28813.t1 MTR_4g121560 46.561 189 98 2 32 217 111 299 8.71e-59 192
MS.gene28813.t1 MTR_3g090120 47.027 185 92 3 35 213 129 313 2.26e-57 189
MS.gene28813.t1 MTR_8g093530 45.161 186 100 1 30 213 85 270 5.48e-57 187
MS.gene28813.t1 MTR_1g058380 44.792 192 101 3 32 218 52 243 1.02e-56 186
MS.gene28813.t1 MTR_5g071350 42.077 183 95 4 35 217 58 229 8.09e-47 159
MS.gene28813.t1 MTR_3g464570 39.891 183 99 4 35 217 43 214 1.01e-45 156
MS.gene28813.t1 MTR_1g112210 37.430 179 101 3 33 211 57 224 8.75e-45 154
MS.gene28813.t1 MTR_7g070060 43.017 179 94 4 34 209 63 236 4.35e-44 150
MS.gene28813.t1 MTR_4g016940 38.889 180 100 3 33 209 84 256 6.89e-44 152
MS.gene28813.t1 MTR_2g091175 41.530 183 95 4 29 211 44 214 7.15e-44 151
MS.gene28813.t1 MTR_4g079700 36.449 214 112 5 1 211 19 211 7.87e-44 150
MS.gene28813.t1 MTR_3g049330 42.021 188 95 5 35 221 31 205 7.97e-44 150
MS.gene28813.t1 MTR_4g014440 40.314 191 104 4 23 209 50 234 8.19e-44 152
MS.gene28813.t1 MTR_1g050448 38.889 180 97 4 33 211 51 218 1.11e-43 150
MS.gene28813.t1 MTR_2g073500 38.743 191 93 6 31 211 25 201 1.80e-43 149
MS.gene28813.t1 MTR_7g070060 43.017 179 94 4 34 209 63 236 7.31e-43 149
MS.gene28813.t1 MTR_5g034810 40.223 179 93 4 33 211 33 197 2.47e-42 147
MS.gene28813.t1 MTR_2g073510 43.575 179 86 6 33 209 31 196 5.26e-42 145
MS.gene28813.t1 MTR_3g021060 42.045 176 88 6 35 209 46 208 6.56e-42 145
MS.gene28813.t1 MTR_3g070900 38.947 190 104 4 33 218 104 285 1.09e-41 147
MS.gene28813.t1 MTR_1g111010 41.530 183 92 5 32 211 29 199 2.53e-41 144
MS.gene28813.t1 MTR_1g112210 37.430 179 88 4 33 211 57 211 2.65e-41 144
MS.gene28813.t1 MTR_2g015720 38.071 197 109 4 31 227 38 221 6.08e-41 143
MS.gene28813.t1 MTR_3g021090 37.853 177 98 3 33 209 29 193 1.06e-40 144
MS.gene28813.t1 MTR_3g035830 37.288 177 99 3 33 209 29 193 2.06e-40 141
MS.gene28813.t1 MTR_8g463790 36.702 188 103 5 30 211 66 243 6.18e-40 142
MS.gene28813.t1 MTR_5g083260 38.378 185 104 3 28 209 76 253 9.04e-40 141
MS.gene28813.t1 MTR_7g068920 36.667 180 104 3 33 209 78 250 9.45e-40 141
MS.gene28813.t1 MTR_4g014430 39.785 186 102 3 33 215 106 284 1.45e-39 141
MS.gene28813.t1 MTR_4g087730 35.450 189 110 3 29 217 39 215 6.28e-39 138
MS.gene28813.t1 MTR_3g071650 40.782 179 94 4 35 209 89 259 1.04e-38 139
MS.gene28813.t1 MTR_2g022580 50.400 125 60 1 95 217 2 126 2.43e-38 129
MS.gene28813.t1 MTR_5g082880 42.162 185 90 6 33 209 163 338 5.00e-38 138
MS.gene28813.t1 MTR_4g088030 39.106 179 97 5 33 211 31 197 1.93e-37 134
MS.gene28813.t1 MTR_6g061710 45.600 125 65 2 9 131 139 262 1.83e-32 123
MS.gene28813.t1 MTR_1g076690 51.685 89 43 0 35 123 223 311 4.68e-30 117
MS.gene28813.t1 MTR_5g035150 36.264 182 101 5 33 209 83 254 4.34e-29 113
MS.gene28813.t1 MTR_5g035080 32.955 176 107 3 35 209 75 240 1.93e-28 111
MS.gene28813.t1 MTR_2g016060 35.638 188 113 5 28 209 47 232 1.98e-28 110
MS.gene28813.t1 MTR_5g035090 33.898 177 109 4 34 209 82 251 6.96e-28 110
MS.gene28813.t1 MTR_7g033230 34.225 187 103 5 29 209 328 500 1.13e-27 110
MS.gene28813.t1 MTR_7g033230 34.225 187 103 5 29 209 258 430 5.57e-27 108
MS.gene28813.t1 MTR_6g016880 35.676 185 102 5 32 209 75 249 8.16e-27 107
MS.gene28813.t1 MTR_8g027900 39.007 141 79 2 28 168 204 337 1.74e-26 107
MS.gene28813.t1 MTR_3g109840 34.078 179 100 5 35 209 73 237 6.82e-26 104
MS.gene28813.t1 MTR_8g104970 33.158 190 113 4 24 210 109 287 6.94e-26 104
MS.gene28813.t1 MTR_7g019140 44.565 92 51 0 32 123 76 167 3.88e-25 97.4
MS.gene28813.t1 MTR_7g019120 31.148 183 115 3 32 210 76 251 1.92e-24 100
MS.gene28813.t1 MTR_5g035120 43.750 96 54 0 33 128 73 168 3.47e-23 92.4
MS.gene28813.t1 MTR_8g099300 32.222 180 111 4 34 209 73 245 5.23e-23 96.3
MS.gene28813.t1 MTR_6g007777 36.000 150 78 5 33 179 114 248 7.97e-23 96.3
MS.gene28813.t1 MTR_6g016875 33.514 185 115 5 29 209 58 238 1.11e-22 95.5
MS.gene28813.t1 MTR_6g016240 32.461 191 104 7 35 209 71 252 5.00e-22 93.6
MS.gene28813.t1 MTR_3g464570 36.719 128 70 4 99 226 3 119 8.33e-22 90.9
MS.gene28813.t1 MTR_5g035070 33.146 178 112 3 35 211 87 258 8.81e-22 93.2
MS.gene28813.t1 MTR_4g092830 31.053 190 111 5 35 213 48 228 2.61e-21 91.7
MS.gene28813.t1 MTR_8g093870 28.788 198 121 4 33 216 131 322 4.71e-21 91.3
MS.gene28813.t1 MTR_7g070060 40.800 125 67 3 87 209 15 134 6.35e-20 86.7
MS.gene28813.t1 MTR_4g125060 28.788 198 121 4 33 216 130 321 9.03e-20 87.4
MS.gene28813.t1 MTR_4g125060 28.788 198 121 4 33 216 198 389 1.21e-19 87.4
MS.gene28813.t1 MTR_4g125060 28.788 198 121 4 33 216 169 360 1.83e-19 86.7
MS.gene28813.t1 MTR_5g069320 31.159 138 67 4 81 217 147 257 7.06e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28813.t1 AT3G62390 68.519 216 63 2 3 218 111 321 1.07e-110 324
MS.gene28813.t1 AT3G62390 68.519 216 63 2 3 218 111 321 1.44e-109 323
MS.gene28813.t1 AT5G19160 57.297 185 77 1 35 217 99 283 9.62e-72 222
MS.gene28813.t1 AT5G19160 57.297 185 77 1 35 217 99 283 3.55e-70 222
MS.gene28813.t1 AT5G20590 54.255 188 84 2 32 217 138 325 9.21e-70 218
MS.gene28813.t1 AT5G20590 54.255 188 84 2 32 217 138 325 1.20e-68 218
MS.gene28813.t1 AT3G06080 55.495 182 79 1 35 214 103 284 1.27e-68 214
MS.gene28813.t1 AT3G06080 55.495 182 79 1 35 214 103 284 1.23e-67 215
MS.gene28813.t1 AT1G60790 53.723 188 84 2 33 217 188 375 3.58e-67 216
MS.gene28813.t1 AT5G06700 51.596 188 88 2 32 217 252 438 5.31e-66 214
MS.gene28813.t1 AT3G12060 50.754 199 92 3 24 217 182 379 7.03e-66 213
MS.gene28813.t1 AT5G49340 47.120 191 98 3 30 217 95 285 1.53e-58 191
MS.gene28813.t1 AT1G48880 45.604 182 97 1 34 213 114 295 3.37e-56 185
MS.gene28813.t1 AT5G06230 42.157 204 110 2 19 214 46 249 9.33e-54 175
MS.gene28813.t1 AT5G06230 42.157 204 110 2 19 214 5 208 4.46e-53 175
MS.gene28813.t1 AT5G06230 42.157 204 110 2 19 214 46 249 1.05e-52 175
MS.gene28813.t1 AT3G11570 48.795 166 83 1 51 214 98 263 1.22e-49 167
MS.gene28813.t1 AT3G54260 41.899 179 93 3 35 213 51 218 2.21e-47 160
MS.gene28813.t1 AT2G38320 42.541 181 94 4 33 209 57 231 8.71e-47 159
MS.gene28813.t1 AT5G58600 39.894 188 101 5 35 221 66 242 1.84e-46 155
MS.gene28813.t1 AT2G40150 41.121 214 108 6 1 209 41 241 1.88e-46 159
MS.gene28813.t1 AT1G29050 41.530 183 96 2 35 217 61 232 9.15e-46 156
MS.gene28813.t1 AT2G34070 38.028 213 118 3 4 216 37 235 1.18e-45 156
MS.gene28813.t1 AT5G58600 39.894 188 101 5 35 221 66 242 1.76e-45 156
MS.gene28813.t1 AT2G40160 41.398 186 94 5 35 214 77 253 3.58e-45 153
MS.gene28813.t1 AT3G55990 43.500 200 95 6 19 209 120 310 4.99e-45 157
MS.gene28813.t1 AT2G30900 40.761 184 97 5 33 215 41 213 1.12e-44 150
MS.gene28813.t1 AT2G30010 41.489 188 96 4 33 217 54 230 1.93e-44 153
MS.gene28813.t1 AT2G30010 41.711 187 95 4 34 217 98 273 2.61e-44 154
MS.gene28813.t1 AT2G40160 41.398 186 94 5 35 214 77 253 4.84e-44 153
MS.gene28813.t1 AT2G40160 41.398 186 94 5 35 214 77 253 6.24e-44 153
MS.gene28813.t1 AT2G30900 40.761 184 97 5 33 215 41 213 2.82e-43 149
MS.gene28813.t1 AT2G31110 38.983 177 96 4 35 211 45 209 4.43e-43 146
MS.gene28813.t1 AT2G42570 39.548 177 95 3 35 211 47 211 7.82e-43 148
MS.gene28813.t1 AT1G73140 36.111 216 116 6 9 212 35 240 2.36e-42 148
MS.gene28813.t1 AT2G31110 38.983 177 96 4 35 211 45 209 6.32e-42 146
MS.gene28813.t1 AT1G78710 37.306 193 107 5 20 211 16 195 2.72e-41 142
MS.gene28813.t1 AT1G78710 37.306 193 107 5 20 211 23 202 1.72e-40 142
MS.gene28813.t1 AT5G01360 36.464 181 103 4 33 209 34 206 4.19e-40 141
MS.gene28813.t1 AT5G01620 39.691 194 104 4 27 215 102 287 5.46e-40 142
MS.gene28813.t1 AT5G01620 39.691 194 104 4 27 215 102 287 5.46e-40 142
MS.gene28813.t1 AT5G01620 39.691 194 104 4 27 215 110 295 6.72e-40 142
MS.gene28813.t1 AT5G01360 36.464 181 103 4 33 209 90 262 1.11e-39 141
MS.gene28813.t1 AT3G11030 38.919 185 103 3 28 209 99 276 4.37e-39 140
MS.gene28813.t1 AT3G14850 36.269 193 111 4 35 227 34 214 2.41e-38 136
MS.gene28813.t1 AT2G40320 36.735 196 111 4 17 209 66 251 4.01e-38 137
MS.gene28813.t1 AT2G38320 39.535 172 92 4 59 227 1 163 5.02e-36 129
MS.gene28813.t1 AT5G51640 35.000 200 109 5 18 210 121 306 5.50e-33 124
MS.gene28813.t1 AT4G25360 49.485 97 49 0 27 123 167 263 6.88e-32 122
MS.gene28813.t1 AT4G25360 49.485 97 49 0 27 123 167 263 6.88e-32 122
MS.gene28813.t1 AT1G70230 39.227 181 96 6 35 211 79 249 1.93e-31 119
MS.gene28813.t1 AT3G28150 37.500 184 104 4 30 209 65 241 8.25e-30 115
MS.gene28813.t1 AT1G01430 33.816 207 119 6 8 209 75 268 5.66e-29 113
MS.gene28813.t1 AT5G15890 34.759 187 113 4 25 210 177 355 9.91e-27 107
MS.gene28813.t1 AT4G11090 35.795 176 97 5 3 169 39 207 3.99e-26 105
MS.gene28813.t1 AT4G23790 33.663 202 118 6 13 209 61 251 7.53e-26 104
MS.gene28813.t1 AT2G14530 30.909 220 125 10 7 209 32 241 1.20e-24 100
MS.gene28813.t1 AT4G01080 32.768 177 108 4 34 209 90 256 2.17e-24 100
MS.gene28813.t1 AT3G02440 30.000 180 112 4 35 209 135 305 1.38e-23 98.6
MS.gene28813.t1 AT3G02440 30.000 180 112 4 35 209 127 297 1.62e-23 98.2
MS.gene28813.t1 AT2G37720 31.959 194 120 5 32 215 140 331 3.80e-23 97.1
MS.gene28813.t1 AT5G64470 33.679 193 107 9 33 213 53 236 1.06e-21 91.7
MS.gene28813.t1 AT5G64470 33.166 199 112 9 35 221 55 244 1.39e-21 91.3
MS.gene28813.t1 AT5G64470 34.031 191 105 9 35 213 55 236 2.01e-21 91.7
MS.gene28813.t1 AT5G64470 34.031 191 105 9 35 213 55 236 2.29e-21 91.7
MS.gene28813.t1 AT5G64020 28.125 192 126 4 35 216 57 246 7.41e-21 90.1
MS.gene28813.t1 AT5G15900 28.889 180 115 4 35 208 67 239 1.73e-20 89.4
MS.gene28813.t1 AT5G15900 28.889 180 115 4 35 208 86 258 1.80e-20 89.4
MS.gene28813.t1 AT5G20680 32.124 193 115 7 33 213 210 398 2.13e-19 86.7
MS.gene28813.t1 AT5G20680 32.124 193 115 7 33 213 210 398 2.13e-19 86.7
MS.gene28813.t1 AT5G20680 32.124 193 115 7 33 213 210 398 2.13e-19 86.7
MS.gene28813.t1 AT5G20680 32.124 193 115 7 33 213 210 398 2.13e-19 86.7
MS.gene28813.t1 AT5G20680 31.771 192 117 6 33 213 192 380 2.14e-19 86.7
MS.gene28813.t1 AT5G20680 31.771 192 117 6 33 213 116 304 2.36e-19 86.3
MS.gene28813.t1 AT2G34070 35.714 112 61 2 105 216 1 101 3.05e-16 75.5
MS.gene28813.t1 AT3G14850 30.894 123 73 4 105 227 1 111 1.51e-11 62.8

Find 40 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTATAGTTTCAGGTTTC+TGG 0.139405 7.4:-5862209 MS.gene28813:intron
TTCCCATTGGTTCCTATTAA+TGG 0.150183 7.4:+5862318 None:intergenic
CCTTGGAAGGTCACAATCTT+TGG 0.179023 7.4:+5863043 None:intergenic
ATGGAGAGGAGCTGATATTT+TGG 0.261754 7.4:-5861970 MS.gene28813:CDS
TCAAAACCTTCATCTATAAA+AGG 0.284074 7.4:+5863119 None:intergenic
AGAGAGGGAACTATAGTTTC+AGG 0.285584 7.4:-5862216 MS.gene28813:CDS
CAAGAATCTCTAGCATAAAT+AGG 0.310027 7.4:+5863143 None:intergenic
TTGTGGGTGATTCCATTAAT+AGG 0.313259 7.4:-5862330 MS.gene28813:CDS
TGGAGTTTATAAGAGGAAAA+AGG 0.315433 7.4:-5862360 MS.gene28813:CDS
ACTATAGTTCCCTCTCTCTT+TGG 0.323759 7.4:+5862222 None:intergenic
GTGATTTAACAAAAGGGTAT+TGG 0.335445 7.4:-5863184 MS.gene28813:CDS
TGATTTAACAAAAGGGTATT+GGG 0.360468 7.4:-5863183 MS.gene28813:CDS
CTTGGAAGGTCACAATCTTT+GGG 0.364337 7.4:+5863044 None:intergenic
AAAGAGTGTGATTTAACAAA+AGG 0.376080 7.4:-5863191 MS.gene28813:CDS
CGTGCCTCATACACTCTCTT+TGG 0.387282 7.4:+5862259 None:intergenic
CGAATTGATGCGATTGATCA+TGG 0.420809 7.4:-5862002 MS.gene28813:CDS
GAGAAGTGCTAACCCAGCTT+TGG 0.426335 7.4:+5861911 None:intergenic
AAAATGTTGGAGTTTATAAG+AGG 0.432567 7.4:-5862367 MS.gene28813:CDS
TTGTGAGGGGAATGGAAGAT+TGG 0.439163 7.4:-5863096 MS.gene28813:CDS
ATCGCATCAATTCGCAAAGT+TGG 0.456158 7.4:+5862011 None:intergenic
AGGAAAATTACCAAAGAGAG+AGG 0.476594 7.4:-5862232 MS.gene28813:CDS
TCACATCACAAAACCAAAGC+TGG 0.490317 7.4:-5861924 MS.gene28813:CDS
TTGATCATGGTTCGTCAAGA+TGG 0.494455 7.4:-5861989 MS.gene28813:CDS
AAGAGTGTGATTTAACAAAA+GGG 0.500174 7.4:-5863190 MS.gene28813:CDS
ATTCCATTAATAGGAACCAA+TGG 0.514445 7.4:-5862321 MS.gene28813:CDS
TGCACAACATTGATTCCCAT+TGG 0.516029 7.4:+5862305 None:intergenic
CCAAAGATTGTGACCTTCCA+AGG 0.518341 7.4:-5863043 MS.gene28813:intron
TGGATAGAAACTTTACTAAG+TGG 0.522870 7.4:-5863076 MS.gene28813:CDS
CATGAAAGCAAAGCAAGAGT+AGG 0.524542 7.4:-5862047 MS.gene28813:CDS
AGATCCAAAGAGAGTGTATG+AGG 0.528363 7.4:-5862263 MS.gene28813:CDS
TTCCATTAATAGGAACCAAT+GGG 0.535430 7.4:-5862320 MS.gene28813:CDS
ATGAAAGCAAAGCAAGAGTA+GGG 0.551438 7.4:-5862046 MS.gene28813:CDS
GAAACTTTACTAAGTGGAGA+TGG 0.558108 7.4:-5863070 MS.gene28813:CDS
CACATCACAAAACCAAAGCT+GGG 0.583781 7.4:-5861923 MS.gene28813:CDS
TTGAGAGAGACTACCTTGGA+AGG 0.601243 7.4:+5863030 None:intergenic
GAGTGTATGAGGCACGTGGA+AGG 0.601700 7.4:-5862252 MS.gene28813:CDS
CATGGTTCGTCAAGATGGAG+AGG 0.632218 7.4:-5861984 MS.gene28813:CDS
GGAAAATTACCAAAGAGAGA+GGG 0.638676 7.4:-5862231 MS.gene28813:CDS
AATGCATGGTAATAGAACAA+AGG 0.639849 7.4:-5863288 MS.gene28813:CDS
AAGAGAGTGTATGAGGCACG+TGG 0.685399 7.4:-5862256 MS.gene28813:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAACAAATATCAAATATTT+TGG + chr7.4:5863049-5863068 None:intergenic 15.0%
!!! GAACAAATATCAAATATTTT+GGG + chr7.4:5863048-5863067 None:intergenic 15.0%
!!! TAAAGTTTTGATTTTTAAGA+AGG - chr7.4:5861943-5861962 MS.gene28813:CDS 15.0%
!! AATGTATAATTGTATATGAC+TGG - chr7.4:5863021-5863040 MS.gene28813:intron 20.0%
!! TAATTATTCAGAACTTTACT+TGG - chr7.4:5862991-5863010 MS.gene28813:intron 20.0%
!!! CAAAAGACATACAATTATTT+TGG - chr7.4:5862512-5862531 MS.gene28813:intron 20.0%
!!! GCAAAGTTAATTTTATTCTT+TGG - chr7.4:5862754-5862773 MS.gene28813:intron 20.0%
! AAAATGTTGGAGTTTATAAG+AGG - chr7.4:5862810-5862829 MS.gene28813:intron 25.0%
! ATAAGTTGTTAATCCAAACA+AGG + chr7.4:5862246-5862265 None:intergenic 25.0%
! ATGTATTGTGTTAGTCAATT+TGG - chr7.4:5862583-5862602 MS.gene28813:intron 25.0%
! ATTATTGACAAGACATACTT+AGG - chr7.4:5862614-5862633 MS.gene28813:intron 25.0%
! CAAAATCATTGTTTACTCTT+AGG - chr7.4:5862703-5862722 MS.gene28813:intron 25.0%
! CAAATAAGTCAATCCAAATA+AGG - chr7.4:5862450-5862469 MS.gene28813:intron 25.0%
! TCAAAACCTTCATCTATAAA+AGG + chr7.4:5862061-5862080 None:intergenic 25.0%
! TTATTGACAAGACATACTTA+GGG - chr7.4:5862615-5862634 MS.gene28813:intron 25.0%
!! AAAGAGTGTGATTTAACAAA+AGG - chr7.4:5861986-5862005 MS.gene28813:CDS 25.0%
!! AAGAGTGTGATTTAACAAAA+GGG - chr7.4:5861987-5862006 MS.gene28813:CDS 25.0%
!! GTCTTTTGTCAATAATTTCA+TGG + chr7.4:5862500-5862519 None:intergenic 25.0%
!!! ATAAGTTTTGTGAGATTGTT+TGG + chr7.4:5862415-5862434 None:intergenic 25.0%
!!! GGATATTATTTTTCTGTCAT+AGG - chr7.4:5862775-5862794 MS.gene28813:intron 25.0%
!!! TAAGTTTTGTGAGATTGTTT+GGG + chr7.4:5862414-5862433 None:intergenic 25.0%
!!! TGATTTAACAAAAGGGTATT+GGG - chr7.4:5861994-5862013 MS.gene28813:CDS 25.0%
!!! TGTTTTAAGTTGTTTTCGTA+AGG - chr7.4:5862385-5862404 MS.gene28813:intron 25.0%
!!! TTTGATTTTTAAGAAGGTTG+AGG - chr7.4:5861949-5861968 MS.gene28813:CDS 25.0%
ATGTATTTCGTTGGTCAATT+TGG - chr7.4:5862473-5862492 MS.gene28813:intron 30.0%
ATTCCATTAATAGGAACCAA+TGG - chr7.4:5862856-5862875 MS.gene28813:intron 30.0%
CAAATAAGGATGTATTTCGT+TGG - chr7.4:5862464-5862483 MS.gene28813:intron 30.0%
CAACGAAATACATCCTTATT+TGG + chr7.4:5862466-5862485 None:intergenic 30.0%
CAAGAATCTCTAGCATAAAT+AGG + chr7.4:5862037-5862056 None:intergenic 30.0%
GTTCAATGCAACAAAAATGT+TGG - chr7.4:5862797-5862816 MS.gene28813:intron 30.0%
TGGAGTTTATAAGAGGAAAA+AGG - chr7.4:5862817-5862836 MS.gene28813:intron 30.0%
TGGATAGAAACTTTACTAAG+TGG - chr7.4:5862101-5862120 MS.gene28813:intron 30.0%
TTCCATTAATAGGAACCAAT+GGG - chr7.4:5862857-5862876 MS.gene28813:intron 30.0%
TTGCAATTGTAGTTATGCTT+AGG - chr7.4:5862220-5862239 MS.gene28813:CDS 30.0%
! GTGATTTAACAAAAGGGTAT+TGG - chr7.4:5861993-5862012 MS.gene28813:CDS 30.0%
! TCTTGTCCTTTTATAGATGA+AGG - chr7.4:5862052-5862071 MS.gene28813:CDS 30.0%
!! GCTGTTTTCATAAGCTATTT+TGG - chr7.4:5862323-5862342 MS.gene28813:CDS 30.0%
AGGAAAATTACCAAAGAGAG+AGG - chr7.4:5862945-5862964 MS.gene28813:intron 35.0%
ATGAAAGCAAAGCAAGAGTA+GGG - chr7.4:5863131-5863150 MS.gene28813:CDS 35.0%
GAAACTTTACTAAGTGGAGA+TGG - chr7.4:5862107-5862126 MS.gene28813:intron 35.0%
GGAAAATTACCAAAGAGAGA+GGG - chr7.4:5862946-5862965 MS.gene28813:intron 35.0%
TCGAAGAAGAACTATAGTGA+CGG - chr7.4:5862660-5862679 MS.gene28813:intron 35.0%
TGTTCTTTCTGCAACTTTGT+AGG - chr7.4:5863063-5863082 MS.gene28813:CDS 35.0%
TTGTGGGTGATTCCATTAAT+AGG - chr7.4:5862847-5862866 MS.gene28813:intron 35.0%
!! GAACTATAGTTTCAGGTTTC+TGG - chr7.4:5862968-5862987 MS.gene28813:intron 35.0%
!! TTCCCATTGGTTCCTATTAA+TGG + chr7.4:5862862-5862881 None:intergenic 35.0%
!!! AGATGAAGGTTTTGATTGTG+AGG - chr7.4:5862066-5862085 MS.gene28813:CDS 35.0%
!!! AGGAAAAAGGCTAGTTTTTG+TGG - chr7.4:5862830-5862849 MS.gene28813:intron 35.0%
!!! ATGAAGGTTTTGATTGTGAG+GGG - chr7.4:5862068-5862087 MS.gene28813:CDS 35.0%
!!! GATGAAGGTTTTGATTGTGA+GGG - chr7.4:5862067-5862086 MS.gene28813:CDS 35.0%
!!! GGAAAAAGGCTAGTTTTTGT+GGG - chr7.4:5862831-5862850 MS.gene28813:intron 35.0%
ACTATAGTTCCCTCTCTCTT+TGG + chr7.4:5862958-5862977 None:intergenic 40.0%
AGAGAGGGAACTATAGTTTC+AGG - chr7.4:5862961-5862980 MS.gene28813:intron 40.0%
ATCGCATCAATTCGCAAAGT+TGG + chr7.4:5863169-5863188 None:intergenic 40.0%
CACATCACAAAACCAAAGCT+GGG - chr7.4:5863254-5863273 MS.gene28813:CDS 40.0%
CATGAAAGCAAAGCAAGAGT+AGG - chr7.4:5863130-5863149 MS.gene28813:CDS 40.0%
CGAATTGATGCGATTGATCA+TGG - chr7.4:5863175-5863194 MS.gene28813:CDS 40.0%
CTTGGAAGGTCACAATCTTT+GGG + chr7.4:5862136-5862155 None:intergenic 40.0%
GAATTTGAGAGAGACTACCT+TGG + chr7.4:5862154-5862173 None:intergenic 40.0%
GGAGTTGCAAAAGTGAAGTT+AGG - chr7.4:5862724-5862743 MS.gene28813:intron 40.0%
TCACATCACAAAACCAAAGC+TGG - chr7.4:5863253-5863272 MS.gene28813:CDS 40.0%
TTGATCATGGTTCGTCAAGA+TGG - chr7.4:5863188-5863207 MS.gene28813:CDS 40.0%
! AGATCCAAAGAGAGTGTATG+AGG - chr7.4:5862914-5862933 MS.gene28813:intron 40.0%
! ATGGAGAGGAGCTGATATTT+TGG - chr7.4:5863207-5863226 MS.gene28813:CDS 40.0%
! TGGTTTTCAACACAGCACAT+TGG - chr7.4:5863227-5863246 MS.gene28813:CDS 40.0%
! TTTTCAACACAGCACATTGG+TGG - chr7.4:5863230-5863249 MS.gene28813:CDS 40.0%
!! AGTTATGCTTAGGCCTTGTT+TGG - chr7.4:5862230-5862249 MS.gene28813:CDS 40.0%
!! TGCACAACATTGATTCCCAT+TGG + chr7.4:5862875-5862894 None:intergenic 40.0%
CCAAAGATTGTGACCTTCCA+AGG - chr7.4:5862134-5862153 MS.gene28813:intron 45.0%
CCTTGGAAGGTCACAATCTT+TGG + chr7.4:5862137-5862156 None:intergenic 45.0%
TTGAGAGAGACTACCTTGGA+AGG + chr7.4:5862150-5862169 None:intergenic 45.0%
TTGTGAGGGGAATGGAAGAT+TGG - chr7.4:5862081-5862100 MS.gene28813:CDS 45.0%
!!! GGTTTTGATTGTGAGGGGAA+TGG - chr7.4:5862073-5862092 MS.gene28813:CDS 45.0%
!!! AAATTCAATATTTTTTTTTT+TGG - chr7.4:5862168-5862187 MS.gene28813:intron 5.0%
CATGGTTCGTCAAGATGGAG+AGG - chr7.4:5863193-5863212 MS.gene28813:CDS 50.0%
CGTGCCTCATACACTCTCTT+TGG + chr7.4:5862921-5862940 None:intergenic 50.0%
! AAGAGAGTGTATGAGGCACG+TGG - chr7.4:5862921-5862940 MS.gene28813:intron 50.0%
!! GAGAAGTGCTAACCCAGCTT+TGG + chr7.4:5863269-5863288 None:intergenic 50.0%
GAGTGTATGAGGCACGTGGA+AGG - chr7.4:5862925-5862944 MS.gene28813:intron 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 5861890 5863309 5861890 ID=MS.gene28813
chr7.4 mRNA 5861890 5863309 5861890 ID=MS.gene28813.t1;Parent=MS.gene28813
chr7.4 exon 5863044 5863309 5863044 ID=MS.gene28813.t1.exon1;Parent=MS.gene28813.t1
chr7.4 CDS 5863044 5863309 5863044 ID=cds.MS.gene28813.t1;Parent=MS.gene28813.t1
chr7.4 exon 5862210 5862402 5862210 ID=MS.gene28813.t1.exon2;Parent=MS.gene28813.t1
chr7.4 CDS 5862210 5862402 5862210 ID=cds.MS.gene28813.t1;Parent=MS.gene28813.t1
chr7.4 exon 5861890 5862114 5861890 ID=MS.gene28813.t1.exon3;Parent=MS.gene28813.t1
chr7.4 CDS 5861890 5862114 5861890 ID=cds.MS.gene28813.t1;Parent=MS.gene28813.t1
Gene Sequence

>MS.gene28813

ATGCATGGTAATAGAACAAAGGTTGAAGTGAGTGCTTTAGTGAGTAATAAGAGTAAAGTTTTGATTTTTAAGAAGGTTGAGGTGAAGAAGATAGTAAAAGAGTGTGATTTAACAAAAGGGTATTGGGTTTTTGATGAAAGTTATCCTATTTATGCTAGAGATTCTTGTCCTTTTATAGATGAAGGTTTTGATTGTGAGGGGAATGGAAGATTGGATAGAAACTTTACTAAGTGGAGATGGCAACCCAAAGATTGTGACCTTCCAAGGTAGTCTCTCTCAAATTCAATATTTTTTTTTTTGGAATACTACTTTTATTTATAGTTTGTCATCTTGCAATTGTAGTTATGCTTAGGCCTTGTTTGGATTAACAACTTATTTGCAATTTAGCATGATAAGCGCAAATAAAGTCAAATTGTTTTTGTATAAGTTATAAGCTGTTTTCATAAGCTATTTTGGAGAGCTTATGAAAATAATTTGAAAACAACTTAATTGACATGTTTTAAGTTGTTTTCGTAAGGTCTCCCAAACAATCTCACAAAACTTATGTCAGTAGATAAGCTCAAATAAGTCAATCCAAATAAGGATGTATTTCGTTGGTCAATTTGGTCCATGAAATTATTGACAAAAGACATACAATTATTTTGGTGAACAGTTATTTTGATTCTTGAACATATAAAAATTGCAAAAACATACATGTATTGTGTTAGTCAATTTGGTCTATAAAATTATTGACAAGACATACTTAGGGATGTTTTTGTAATTTTGATACATCGAAGAAGAACTATAGTGACGGTAACTCACACATTTAGAGATCAAAATCATTGTTTACTCTTAGGAGTTGCAAAAGTGAAGTTAGGAACAACTGCAAAGTTAATTTTATTCTTTGGATATTATTTTTCTGTCATAGGTTCAATGCAACAAAAATGTTGGAGTTTATAAGAGGAAAAAGGCTAGTTTTTGTGGGTGATTCCATTAATAGGAACCAATGGGAATCAATGTTGTGCATGTTATTGAGTGCTGTTAAAGATCCAAAGAGAGTGTATGAGGCACGTGGAAGGAAAATTACCAAAGAGAGAGGGAACTATAGTTTCAGGTTTCTGGTAATTATTCAGAACTTTACTTGGTTTGTGTAATGTATAATTGTATATGACTGGTCCCAAAATATTTGATATTTGTTCTTTCTGCAACTTTGTAGGACTATCAATGTACAGTTGAATACTATGTGAGTCATTTCTTAGTTCATGAAAGCAAAGCAAGAGTAGGGCAGAAACGAAGACCAACTTTGCGAATTGATGCGATTGATCATGGTTCGTCAAGATGGAGAGGAGCTGATATTTTGGTTTTCAACACAGCACATTGGTGGTCACATCACAAAACCAAAGCTGGGTTAGCACTTCTCTCTATCTTTTCGAAATTGTAG
Protein sequence

>MS.gene28813.t1

MHGNRTKVEVSALVSNKSKVLIFKKVEVKKIVKECDLTKGYWVFDESYPIYARDSCPFIDEGFDCEGNGRLDRNFTKWRWQPKDCDLPRFNATKMLEFIRGKRLVFVGDSINRNQWESMLCMLLSAVKDPKRVYEARGRKITKERGNYSFRFLDYQCTVEYYVSHFLVHESKARVGQKRRPTLRIDAIDHGSSRWRGADILVFNTAHWWSHHKTKAGLALLSIFSKL