AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29137

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29137.t1 MTR_5g032420 98.447 322 5 0 1 322 1 322 0.0 644
MS.gene29137.t1 MTR_8g068890 81.366 322 53 4 1 322 1 315 1.23e-178 496
MS.gene29137.t1 MTR_3g100570 75.841 327 60 7 1 322 1 313 2.41e-170 475
MS.gene29137.t1 MTR_8g086070 38.006 321 159 3 3 322 18 299 3.32e-69 218
MS.gene29137.t1 MTR_7g066690 35.873 315 171 3 6 320 17 300 3.44e-64 205
MS.gene29137.t1 MTR_4g018750 35.016 317 175 3 6 322 15 300 2.84e-62 200
MS.gene29137.t1 MTR_1g090330 32.039 309 178 3 11 318 19 296 1.27e-53 178
MS.gene29137.t1 MTR_3g462140 32.704 318 182 3 4 320 13 299 1.17e-51 172
MS.gene29137.t1 MTR_8g086070 38.813 219 95 2 3 220 18 198 3.63e-46 155
MS.gene29137.t1 MTR_4g018750 33.214 280 156 3 6 285 15 263 1.63e-45 156
MS.gene29137.t1 MTR_4g018750 29.121 182 106 6 150 317 31 203 2.55e-14 72.4
MS.gene29137.t1 MTR_1g088995 30.671 313 178 6 6 318 33 306 1.74e-41 146
MS.gene29137.t1 MTR_1g088995 29.258 229 124 5 6 234 33 223 1.78e-21 91.7
MS.gene29137.t1 MTR_4g057850 25.552 317 188 9 7 315 23 299 1.26e-19 87.8
MS.gene29137.t1 MTR_4g057850 26.087 184 129 2 127 309 15 192 2.11e-11 64.3
MS.gene29137.t1 MTR_4g012850 22.327 318 202 7 6 319 16 292 1.35e-16 79.3
MS.gene29137.t1 MTR_3g104500 23.922 255 178 7 73 320 77 322 3.05e-16 78.6
MS.gene29137.t1 MTR_5g009500 23.754 341 199 9 3 319 35 338 3.28e-15 75.9
MS.gene29137.t1 MTR_5g009500 28.000 200 127 6 131 319 31 224 2.44e-11 64.3
MS.gene29137.t1 MTR_5g055410 26.316 304 183 7 22 319 37 305 3.73e-15 75.5
MS.gene29137.t1 MTR_7g117030 24.771 327 217 11 12 321 23 337 3.74e-14 72.8
MS.gene29137.t1 MTR_7g117030 26.106 226 128 7 134 322 14 237 6.57e-13 68.9
MS.gene29137.t1 MTR_8g027975 25.217 345 191 13 3 319 48 353 1.16e-13 71.2
MS.gene29137.t1 MTR_8g027975 26.633 199 131 4 131 319 44 237 1.72e-12 67.8
MS.gene29137.t1 MTR_5g073040 25.538 325 182 9 11 322 40 317 1.88e-13 70.5
MS.gene29137.t1 MTR_1g087840 25.249 301 190 10 22 319 35 303 2.03e-13 70.1
MS.gene29137.t1 MTR_2g102010 23.364 321 200 7 6 321 17 296 2.14e-13 70.1
MS.gene29137.t1 MTR_1g097890 27.586 232 150 5 90 319 89 304 2.92e-13 69.7
MS.gene29137.t1 MTR_4g088545 27.225 191 130 4 131 316 9 195 3.39e-13 68.6
MS.gene29137.t1 MTR_6g091800 25.481 208 125 6 128 317 12 207 3.96e-13 68.9
MS.gene29137.t1 MTR_6g034515 25.574 305 170 11 21 316 82 338 4.23e-13 69.7
MS.gene29137.t1 MTR_4g088545 27.225 191 130 4 131 316 9 195 4.59e-13 68.6
MS.gene29137.t1 MTR_6g091800 25.604 207 126 6 128 317 12 207 5.32e-13 68.6
MS.gene29137.t1 MTR_4g119770 21.765 340 216 9 5 322 17 328 5.93e-13 68.9
MS.gene29137.t1 MTR_4g088545 27.225 191 130 4 131 316 9 195 6.37e-13 68.6
MS.gene29137.t1 MTR_4g088545 23.565 331 202 11 2 319 8 300 9.33e-11 62.4
MS.gene29137.t1 MTR_6g091800 25.604 207 126 6 128 317 12 207 9.11e-13 68.2
MS.gene29137.t1 MTR_5g030530 24.611 321 194 9 9 319 14 296 9.83e-13 68.2
MS.gene29137.t1 MTR_7g068340 28.161 174 114 4 150 321 103 267 1.70e-12 67.8
MS.gene29137.t1 MTR_7g064980 20.859 326 222 6 3 322 22 317 4.33e-12 66.2
MS.gene29137.t1 MTR_2g098070 25.214 234 160 7 67 293 102 327 4.79e-12 66.6
MS.gene29137.t1 MTR_7g109135 23.492 315 206 7 8 319 24 306 1.17e-11 65.1
MS.gene29137.t1 MTR_8g066975 25.776 322 189 10 9 319 14 296 4.12e-11 63.2
MS.gene29137.t1 MTR_8g076760 26.908 249 162 9 85 320 65 306 4.68e-11 63.2
MS.gene29137.t1 MTR_6g091800 26.230 183 107 6 128 293 12 183 7.91e-11 60.8
MS.gene29137.t1 MTR_3g098460 27.660 188 125 6 138 322 205 384 8.38e-11 63.2
MS.gene29137.t1 MTR_3g028590 23.355 304 189 9 6 300 97 365 8.65e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29137.t1 AT4G24570 74.224 322 74 4 1 322 1 313 1.97e-168 471
MS.gene29137.t1 AT2G22500 75.232 323 67 6 1 322 1 311 2.43e-164 460
MS.gene29137.t1 AT5G09470 60.117 341 109 8 1 321 1 334 9.62e-125 361
MS.gene29137.t1 AT5G19760 38.272 324 150 4 3 320 15 294 2.43e-70 221
MS.gene29137.t1 AT3G54110 36.677 319 171 4 4 322 13 300 7.77e-67 212
MS.gene29137.t1 AT5G58970 36.308 325 158 5 6 319 12 298 9.84e-66 209
MS.gene29137.t1 AT5G58970 26.222 225 115 7 9 228 127 305 1.20e-12 67.8
MS.gene29137.t1 AT1G14140 33.441 311 172 5 11 318 21 299 9.24e-54 178
MS.gene29137.t1 AT4G03115 32.288 319 168 7 3 318 35 308 6.51e-47 160
MS.gene29137.t1 AT4G03115 32.288 319 168 7 3 318 67 340 1.35e-46 161
MS.gene29137.t1 AT5G58970 35.385 260 126 4 6 254 12 240 6.94e-45 154
MS.gene29137.t1 AT5G58970 26.012 173 123 3 150 317 32 204 3.12e-16 77.8
MS.gene29137.t1 AT4G03115 33.716 261 152 6 61 318 3 245 2.08e-42 147
MS.gene29137.t1 AT4G03115 34.156 243 142 5 79 318 10 237 1.59e-40 142
MS.gene29137.t1 AT4G03115 30.043 233 120 5 3 234 35 225 5.75e-25 100
MS.gene29137.t1 AT4G03115 29.614 233 121 5 3 234 35 225 6.85e-25 100
MS.gene29137.t1 AT5G01340 26.250 320 193 8 7 320 18 300 2.10e-20 90.1
MS.gene29137.t1 AT5G01340 26.178 191 134 2 120 309 3 187 8.19e-12 65.5
MS.gene29137.t1 AT5G46800 26.235 324 188 9 9 319 14 299 1.26e-17 82.0
MS.gene29137.t1 AT5G46800 21.717 198 146 2 131 319 5 202 2.79e-11 63.9
MS.gene29137.t1 AT5G46800 26.235 324 188 9 9 319 14 299 1.26e-17 82.0
MS.gene29137.t1 AT5G46800 21.717 198 146 2 131 319 5 202 2.79e-11 63.9
MS.gene29137.t1 AT5G46800 26.235 324 188 9 9 319 14 299 1.26e-17 82.0
MS.gene29137.t1 AT5G46800 21.717 198 146 2 131 319 5 202 2.79e-11 63.9
MS.gene29137.t1 AT5G56450 25.969 258 169 7 73 320 81 326 7.17e-17 80.5
MS.gene29137.t1 AT1G25380 25.413 303 190 7 22 320 37 307 1.13e-14 74.3
MS.gene29137.t1 AT1G25380 25.413 303 190 7 22 320 37 307 1.13e-14 74.3
MS.gene29137.t1 AT4G26180 28.431 204 126 7 127 317 13 209 1.93e-14 72.8
MS.gene29137.t1 AT1G07030 26.911 327 186 10 6 322 36 319 3.32e-14 72.8
MS.gene29137.t1 AT4G26180 28.431 204 126 7 127 317 13 209 3.34e-14 72.8
MS.gene29137.t1 AT3G51870 29.480 173 113 3 150 321 107 271 9.41e-14 71.6
MS.gene29137.t1 AT3G51870 22.517 302 186 9 20 319 105 360 1.17e-11 65.5
MS.gene29137.t1 AT1G34065 25.000 320 176 9 4 321 73 330 1.51e-13 70.9
MS.gene29137.t1 AT1G34065 25.000 320 176 9 4 321 80 337 1.88e-13 70.5
MS.gene29137.t1 AT2G33820 24.551 334 193 13 5 319 13 306 2.15e-13 70.1
MS.gene29137.t1 AT3G53940 24.352 193 129 4 138 318 73 260 1.22e-12 67.8
MS.gene29137.t1 AT3G53940 24.352 193 129 4 138 318 73 260 1.32e-12 68.2
MS.gene29137.t1 AT3G53940 23.030 330 202 8 3 319 70 360 1.89e-12 67.8
MS.gene29137.t1 AT4G01100 26.012 346 185 14 4 319 40 344 1.62e-12 67.8
MS.gene29137.t1 AT4G01100 26.396 197 134 3 131 319 35 228 2.10e-12 67.4
MS.gene29137.t1 AT5G01500 27.119 177 120 3 146 321 131 299 2.95e-12 67.4
MS.gene29137.t1 AT5G01500 24.000 300 184 7 20 319 133 388 3.51e-11 64.3
MS.gene29137.t1 AT4G27940 22.701 348 220 10 12 320 71 408 3.10e-12 67.4
MS.gene29137.t1 AT5G66380 28.718 195 121 8 136 322 12 196 3.31e-12 66.6
MS.gene29137.t1 AT3G55640 23.590 195 134 3 138 321 38 228 3.96e-12 65.9
MS.gene29137.t1 AT3G55640 23.590 195 134 3 138 321 38 228 3.96e-12 65.9
MS.gene29137.t1 AT4G01100 27.881 269 156 11 80 319 35 294 6.52e-12 65.9
MS.gene29137.t1 AT2G39970 23.656 279 178 8 71 317 44 319 8.55e-12 65.5
MS.gene29137.t1 AT5G17400 22.712 295 188 7 6 293 14 275 8.64e-12 65.5
MS.gene29137.t1 AT5G51050 29.101 189 123 6 138 322 223 404 1.78e-11 65.1
MS.gene29137.t1 AT2G26360 22.910 323 181 11 3 317 112 374 1.78e-11 65.1
MS.gene29137.t1 AT2G33820 25.728 206 141 6 116 316 3 201 2.38e-11 63.5
MS.gene29137.t1 AT4G01100 25.648 347 201 17 3 319 39 358 2.51e-11 64.3
MS.gene29137.t1 AT3G21390 22.659 331 202 9 9 313 22 324 5.88e-11 63.2
MS.gene29137.t1 AT3G21390 22.659 331 202 9 9 313 44 346 7.56e-11 62.8
MS.gene29137.t1 AT4G28390 22.185 302 195 7 6 300 83 351 8.31e-11 62.8
MS.gene29137.t1 AT4G28390 22.185 302 195 7 6 300 83 351 8.31e-11 62.8

Find 118 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTATTCCCACAATTTCT+AGG 0.306500 5.3:+21524237 MS.gene29137:CDS
CACATGGATCGTGGTTGTTC+CGG 0.314214 5.3:-21523523 None:intergenic
CTCCGACCGGCACTTGCTTT+CGG 0.332288 5.3:+21523495 MS.gene29137:CDS
CTGCTTCGCCATTCTGGTAA+TGG 0.333635 5.3:-21523892 None:intergenic
GGACTTATTGCCGGCGGGGT+TGG 0.357971 5.3:+21523768 MS.gene29137:CDS
TGTTGTTCTGTTTGTTACTT+TGG 0.360137 5.3:+21524274 MS.gene29137:CDS
ACATCCACTAGATCTTATTA+AGG 0.364470 5.3:+21523419 MS.gene29137:CDS
ACCACCTTATGCTGGTGCTT+TGG 0.365642 5.3:+21524163 MS.gene29137:CDS
ACCTTCCTGCTTCGCCATTC+TGG 0.380086 5.3:-21523898 None:intergenic
GGTGTTATGCGTGATGGGCT+TGG 0.402456 5.3:+21524029 MS.gene29137:CDS
CGGAGGATCTGAACCGGGTT+CGG 0.406065 5.3:-21523477 None:intergenic
ATCGAGGCTGGACTTATTGC+CGG 0.411260 5.3:+21523759 MS.gene29137:CDS
GTGTTATGCGTGATGGGCTT+GGG 0.421903 5.3:+21524030 MS.gene29137:CDS
CCAACACGAGGCTGAGGAAC+CGG 0.438856 5.3:-21523555 None:intergenic
CGAACCATGGCAACATCGGC+GGG 0.449170 5.3:-21523807 None:intergenic
GCCGGTCGGAGGATCTGAAC+CGG 0.451478 5.3:-21523483 None:intergenic
TATTCCCACAATTTCTAGGC+AGG 0.452419 5.3:+21524241 MS.gene29137:CDS
GGTGTAACAGCTTTGTTCTC+CGG 0.452972 5.3:+21523615 MS.gene29137:CDS
GGCGGGGTTGGTGCTGCTAT+CGG 0.453712 5.3:+21523780 MS.gene29137:CDS
GCATCTCCGCCACTGTGCTC+AGG 0.455298 5.3:+21523637 MS.gene29137:CDS
CTGTTTCGCAAGATCGAGGC+TGG 0.456258 5.3:+21523747 MS.gene29137:CDS
GTTGGTCACAGCTTCACAGC+TGG 0.456447 5.3:+21523968 MS.gene29137:CDS
GAGCACAGTGGCGGAGATGC+CGG 0.463595 5.3:-21523634 None:intergenic
ATCTGAACCGGGTTCGGCTT+AGG 0.465925 5.3:-21523471 None:intergenic
AAGGTTCGCATGCAACTTCA+AGG 0.468746 5.3:+21523438 MS.gene29137:CDS
GGCGGCTGTTGCGTCGAATC+CGG 0.475424 5.3:+21524088 MS.gene29137:CDS
GCACTTGCTTTCGGTCAAAC+CGG 0.477758 5.3:+21523504 MS.gene29137:CDS
AGCCGATGGGAGACTCCCAC+AGG 0.481179 5.3:+21523836 MS.gene29137:CDS
CATTACCAGAATGGCGAAGC+AGG 0.481369 5.3:+21523893 MS.gene29137:CDS
CAATCCAAAGCACCAGCATA+AGG 0.486227 5.3:-21524167 None:intergenic
GTGTGACCAGTCTGTGGCGC+GGG 0.488791 5.3:+21523919 MS.gene29137:CDS
GCAAGTTCGCAAGTTGTTGA+AGG 0.493746 5.3:+21524298 MS.gene29137:CDS
CAAGGAGATGATTCTTGAGA+AGG 0.493818 5.3:+21524007 MS.gene29137:CDS
ATGAACATGAAGGTGGAGGT+TGG 0.497583 5.3:+21524134 MS.gene29137:CDS
CTGCAAAACTAGCTGTCACA+TGG 0.501796 5.3:-21524055 None:intergenic
CTCTACTCCACCACCAGGAT+GGG 0.504042 5.3:+21523666 MS.gene29137:CDS
AGGTCCCTGCCTAGAAATTG+TGG 0.505879 5.3:-21524246 None:intergenic
GGGTCCCATGGCTCTATACA+AGG 0.509423 5.3:+21524214 MS.gene29137:CDS
GTAGAGTCCCATCCTGGTGG+TGG 0.512466 5.3:-21523673 None:intergenic
TAGTTTCTACGCTGAGCCTG+TGG 0.518837 5.3:-21523852 None:intergenic
AGGCTGGACTTATTGCCGGC+GGG 0.524366 5.3:+21523763 MS.gene29137:CDS
CGATGACCCGCGCCACAGAC+TGG 0.524749 5.3:-21523925 None:intergenic
GGCTTCATATGATCAATTCA+AGG 0.534751 5.3:+21523989 MS.gene29137:CDS
CATGAAGGTGGAGGTTGGAA+AGG 0.542492 5.3:+21524139 MS.gene29137:CDS
AAATCCCTTGTATAGAGCCA+TGG 0.543025 5.3:-21524219 None:intergenic
AAGTGGACGGATCGTGAAGC+CGG 0.545520 5.3:+21523711 MS.gene29137:CDS
CGGTGGATGTTATTAAGACA+AGG 0.547567 5.3:+21524108 MS.gene29137:CDS
AGAAGGGTGTTATGCGTGAT+GGG 0.547920 5.3:+21524024 MS.gene29137:CDS
TTGAAGACAGTTCGTGCTGA+GGG 0.548173 5.3:+21524194 MS.gene29137:CDS
GGAAAGGAACCACCTTATGC+TGG 0.548867 5.3:+21524155 MS.gene29137:CDS
TAACCCCGCCGATGTTGCCA+TGG 0.550940 5.3:+21523803 MS.gene29137:CDS
CATTCGAACCATGGCAACAT+CGG 0.551061 5.3:-21523811 None:intergenic
CGTGTTGGCCTTATCTCAGT+CGG 0.555631 5.3:+21523570 MS.gene29137:CDS
GAGAAGGGTGTTATGCGTGA+TGG 0.556651 5.3:+21524023 MS.gene29137:CDS
GGTCCCTGCCTAGAAATTGT+GGG 0.557689 5.3:-21524245 None:intergenic
GTCCGTCTTGTTCAACAAGA+AGG 0.557738 5.3:+21523594 MS.gene29137:CDS
CACCTTCTTGTTGAACAAGA+CGG 0.561342 5.3:-21523596 None:intergenic
CCGGTTCCTCAGCCTCGTGT+TGG 0.563139 5.3:+21523555 MS.gene29137:CDS
ATGGTTCGAATGCAAGCCGA+TGG 0.563779 5.3:+21523822 MS.gene29137:CDS
AAACAGCGGCATAGTGCCAC+CGG 0.564991 5.3:-21523730 None:intergenic
AAGGAGATGATTCTTGAGAA+GGG 0.566148 5.3:+21524008 MS.gene29137:CDS
GACGGACTCCGACTGAGATA+AGG 0.567027 5.3:-21523578 None:intergenic
AGTTCGTGCTGAGGGTCCCA+TGG 0.567299 5.3:+21524202 MS.gene29137:CDS
AACTTCGTAGAGTCCCATCC+TGG 0.571198 5.3:-21523679 None:intergenic
AATCCCTTGTATAGAGCCAT+GGG 0.571325 5.3:-21524218 None:intergenic
CTTGTCTTAATAACATCCAC+CGG 0.572366 5.3:-21524107 None:intergenic
ATCGGCTTGCATTCGAACCA+TGG 0.573789 5.3:-21523820 None:intergenic
TCGAACCATGGCAACATCGG+CGG 0.579571 5.3:-21523808 None:intergenic
GATCCATGTGGGGTCTACTC+CGG 0.580439 5.3:+21523536 MS.gene29137:CDS
ACAAACAGAACAACAGTGAA+AGG 0.581480 5.3:-21524266 None:intergenic
AGCCTGTGGGAGTCTCCCAT+CGG 0.586325 5.3:-21523838 None:intergenic
CGAACCTTAATAAGATCTAG+TGG 0.587146 5.3:-21523423 None:intergenic
GGCTGGACTTATTGCCGGCG+GGG 0.587219 5.3:+21523764 MS.gene29137:CDS
ATAAGGCCAACACGAGGCTG+AGG 0.587508 5.3:-21523561 None:intergenic
GACTGTGAACCGCGCCATGT+TGG 0.590940 5.3:+21523950 MS.gene29137:CDS
ACTCTACTCCACCACCAGGA+TGG 0.591202 5.3:+21523665 MS.gene29137:CDS
CGAAAGCAAGTGCCGGTCGG+AGG 0.591618 5.3:-21523494 None:intergenic
GGTGTGACCAGTCTGTGGCG+CGG 0.596360 5.3:+21523918 MS.gene29137:CDS
AGTTTCTACGCTGAGCCTGT+GGG 0.598129 5.3:-21523851 None:intergenic
GGTATTGCTTCAATCATCGC+AGG 0.598971 5.3:+21523387 MS.gene29137:CDS
AATAAGATCTAGTGGATGTG+TGG 0.600430 5.3:-21523415 None:intergenic
CCGGTCGGAGGATCTGAACC+GGG 0.601331 5.3:-21523482 None:intergenic
CCCGGTTCAGATCCTCCGAC+CGG 0.604021 5.3:+21523482 MS.gene29137:CDS
GCCTCGATCTTGCGAAACAG+CGG 0.604552 5.3:-21523744 None:intergenic
GTTGAAGACAGTTCGTGCTG+AGG 0.606713 5.3:+21524193 MS.gene29137:CDS
ATTCCCACAATTTCTAGGCA+GGG 0.607281 5.3:+21524242 MS.gene29137:CDS
GAACCGGAGTAGACCCCACA+TGG 0.607542 5.3:-21523539 None:intergenic
TGTCGACGCCATTACCAGAA+TGG 0.612357 5.3:+21523884 MS.gene29137:CDS
GCCGCTGTTTCGCAAGATCG+AGG 0.616216 5.3:+21523743 MS.gene29137:CDS
ACCAGAATGGCGAAGCAGGA+AGG 0.618327 5.3:+21523897 MS.gene29137:CDS
GTTTGACCGAAAGCAAGTGC+CGG 0.620216 5.3:-21523501 None:intergenic
GATAGCAGCACCAACCCCGC+CGG 0.621207 5.3:-21523778 None:intergenic
GACCGAAAGCAAGTGCCGGT+CGG 0.622497 5.3:-21523497 None:intergenic
AGGAAGGTGTGACCAGTCTG+TGG 0.623057 5.3:+21523913 MS.gene29137:CDS
AAACGCGCCTAAGCCGAACC+CGG 0.629487 5.3:+21523464 MS.gene29137:CDS
TGTCTGCCTGAGCACAGTGG+CGG 0.630046 5.3:-21523643 None:intergenic
GGAACAACCACGATCCATGT+GGG 0.630425 5.3:+21523525 MS.gene29137:CDS
TGGTTCGAATGCAAGCCGAT+GGG 0.641550 5.3:+21523823 MS.gene29137:CDS
CGGAACAACCACGATCCATG+TGG 0.645591 5.3:+21523524 MS.gene29137:CDS
AAGGGTGATGAACATGAAGG+TGG 0.649113 5.3:+21524127 MS.gene29137:CDS
TGGACGGATCGTGAAGCCGG+TGG 0.650308 5.3:+21523714 MS.gene29137:CDS
GAGGCTGGACTTATTGCCGG+CGG 0.651692 5.3:+21523762 MS.gene29137:CDS
AGTAGACCCCACATGGATCG+TGG 0.652877 5.3:-21523532 None:intergenic
GGCTGTTGCGTCGAATCCGG+TGG 0.655060 5.3:+21524091 MS.gene29137:CDS
CTGTGAAGCTGTGACCAACA+TGG 0.655608 5.3:-21523964 None:intergenic
TCCAAAGCACCAGCATAAGG+TGG 0.656451 5.3:-21524164 None:intergenic
TTCGTAGAGTCCCATCCTGG+TGG 0.665174 5.3:-21523676 None:intergenic
GACAAGGGTGATGAACATGA+AGG 0.670089 5.3:+21524124 MS.gene29137:CDS
AGACACTCTACTCCACCACC+AGG 0.671703 5.3:+21523661 MS.gene29137:CDS
GGTGATGAACATGAAGGTGG+AGG 0.674760 5.3:+21524130 MS.gene29137:CDS
TGCAAAACTAGCTGTCACAT+GGG 0.682381 5.3:-21524054 None:intergenic
GGTGGATGTTATTAAGACAA+GGG 0.685805 5.3:+21524109 MS.gene29137:CDS
GAACCATGGCAACATCGGCG+GGG 0.689811 5.3:-21523806 None:intergenic
GGTCCCATGGCTCTATACAA+GGG 0.689831 5.3:+21524215 MS.gene29137:CDS
GAACAACCACGATCCATGTG+GGG 0.695252 5.3:+21523526 MS.gene29137:CDS
AAGCTGTGACCAACATGGCG+CGG 0.720195 5.3:-21523959 None:intergenic
ACTGAGATAAGGCCAACACG+AGG 0.747866 5.3:-21523567 None:intergenic
GAGTGTCTGCCTGAGCACAG+TGG 0.754735 5.3:-21523646 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACATCCACTAGATCTTATTA+AGG + chr5.3:21523419-21523438 MS.gene29137:CDS 30.0%
GATTTATTCCCACAATTTCT+AGG + chr5.3:21524237-21524256 MS.gene29137:CDS 30.0%
! TGTTGTTCTGTTTGTTACTT+TGG + chr5.3:21524274-21524293 MS.gene29137:CDS 30.0%
AAGGAGATGATTCTTGAGAA+GGG + chr5.3:21524008-21524027 MS.gene29137:CDS 35.0%
ACAAACAGAACAACAGTGAA+AGG - chr5.3:21524269-21524288 None:intergenic 35.0%
CGAACCTTAATAAGATCTAG+TGG - chr5.3:21523426-21523445 None:intergenic 35.0%
CTTGTCTTAATAACATCCAC+CGG - chr5.3:21524110-21524129 None:intergenic 35.0%
GGCTTCATATGATCAATTCA+AGG + chr5.3:21523989-21524008 MS.gene29137:CDS 35.0%
GGTGGATGTTATTAAGACAA+GGG + chr5.3:21524109-21524128 MS.gene29137:CDS 35.0%
! AATAAGATCTAGTGGATGTG+TGG - chr5.3:21523418-21523437 None:intergenic 35.0%
!! ACGAAGTTCTGAAAAACAAG+TGG + chr5.3:21523694-21523713 MS.gene29137:CDS 35.0%
!! AGTTCTGAAAAACAAGTGGA+CGG + chr5.3:21523698-21523717 MS.gene29137:CDS 35.0%
AAATCCCTTGTATAGAGCCA+TGG - chr5.3:21524222-21524241 None:intergenic 40.0%
AATCCCTTGTATAGAGCCAT+GGG - chr5.3:21524221-21524240 None:intergenic 40.0%
CAAGGAGATGATTCTTGAGA+AGG + chr5.3:21524007-21524026 MS.gene29137:CDS 40.0%
CACCTTCTTGTTGAACAAGA+CGG - chr5.3:21523599-21523618 None:intergenic 40.0%
CGGTGGATGTTATTAAGACA+AGG + chr5.3:21524108-21524127 MS.gene29137:CDS 40.0%
TGCAAAACTAGCTGTCACAT+GGG - chr5.3:21524057-21524076 None:intergenic 40.0%
! ATTCCCACAATTTCTAGGCA+GGG + chr5.3:21524242-21524261 MS.gene29137:CDS 40.0%
! TATTCCCACAATTTCTAGGC+AGG + chr5.3:21524241-21524260 MS.gene29137:CDS 40.0%
!!! GGTGTCAAAGGTTTTGTTGA+AGG + chr5.3:21523363-21523382 MS.gene29137:CDS 40.0%
!!! GTCAAAGGTTTTGTTGAAGG+TGG + chr5.3:21523366-21523385 MS.gene29137:CDS 40.0%
AAGGGTGATGAACATGAAGG+TGG + chr5.3:21524127-21524146 MS.gene29137:CDS 45.0%
AAGGTTCGCATGCAACTTCA+AGG + chr5.3:21523438-21523457 MS.gene29137:CDS 45.0%
ATGAACATGAAGGTGGAGGT+TGG + chr5.3:21524134-21524153 MS.gene29137:CDS 45.0%
CATTCGAACCATGGCAACAT+CGG - chr5.3:21523814-21523833 None:intergenic 45.0%
CTGCAAAACTAGCTGTCACA+TGG - chr5.3:21524058-21524077 None:intergenic 45.0%
GACAAGGGTGATGAACATGA+AGG + chr5.3:21524124-21524143 MS.gene29137:CDS 45.0%
GCAAGTTCGCAAGTTGTTGA+AGG + chr5.3:21524298-21524317 MS.gene29137:CDS 45.0%
GGTATTGCTTCAATCATCGC+AGG + chr5.3:21523387-21523406 MS.gene29137:CDS 45.0%
TTGAAGACAGTTCGTGCTGA+GGG + chr5.3:21524194-21524213 MS.gene29137:CDS 45.0%
! CAATCCAAAGCACCAGCATA+AGG - chr5.3:21524170-21524189 None:intergenic 45.0%
! GTCCGTCTTGTTCAACAAGA+AGG + chr5.3:21523594-21523613 MS.gene29137:CDS 45.0%
!! AGAAGGGTGTTATGCGTGAT+GGG + chr5.3:21524024-21524043 MS.gene29137:CDS 45.0%
!! GGTGTAACAGCTTTGTTCTC+CGG + chr5.3:21523615-21523634 MS.gene29137:CDS 45.0%
!! TAGTTTTGCAGCGGGATTTG+TGG + chr5.3:21524067-21524086 MS.gene29137:CDS 45.0%
!!! TGTGACAGCTAGTTTTGCAG+CGG + chr5.3:21524058-21524077 MS.gene29137:CDS 45.0%
AACTTCGTAGAGTCCCATCC+TGG - chr5.3:21523682-21523701 None:intergenic 50.0%
ACCACCTTATGCTGGTGCTT+TGG + chr5.3:21524163-21524182 MS.gene29137:CDS 50.0%
ACTGAGATAAGGCCAACACG+AGG - chr5.3:21523570-21523589 None:intergenic 50.0%
AGGTCCCTGCCTAGAAATTG+TGG - chr5.3:21524249-21524268 None:intergenic 50.0%
AGTTTCTACGCTGAGCCTGT+GGG - chr5.3:21523854-21523873 None:intergenic 50.0%
ATCGAGGCTGGACTTATTGC+CGG + chr5.3:21523759-21523778 MS.gene29137:CDS 50.0%
ATGGTTCGAATGCAAGCCGA+TGG + chr5.3:21523822-21523841 MS.gene29137:CDS 50.0%
CACATGGATCGTGGTTGTTC+CGG - chr5.3:21523526-21523545 None:intergenic 50.0%
CATGAAGGTGGAGGTTGGAA+AGG + chr5.3:21524139-21524158 MS.gene29137:CDS 50.0%
CATTACCAGAATGGCGAAGC+AGG + chr5.3:21523893-21523912 MS.gene29137:CDS 50.0%
CTGCTTCGCCATTCTGGTAA+TGG - chr5.3:21523895-21523914 None:intergenic 50.0%
CTGTGAAGCTGTGACCAACA+TGG - chr5.3:21523967-21523986 None:intergenic 50.0%
GAACAACCACGATCCATGTG+GGG + chr5.3:21523526-21523545 MS.gene29137:CDS 50.0%
GCACTTGCTTTCGGTCAAAC+CGG + chr5.3:21523504-21523523 MS.gene29137:CDS 50.0%
GGAAAGGAACCACCTTATGC+TGG + chr5.3:21524155-21524174 MS.gene29137:CDS 50.0%
GGAACAACCACGATCCATGT+GGG + chr5.3:21523525-21523544 MS.gene29137:CDS 50.0%
GGTCCCTGCCTAGAAATTGT+GGG - chr5.3:21524248-21524267 None:intergenic 50.0%
GGTGATGAACATGAAGGTGG+AGG + chr5.3:21524130-21524149 MS.gene29137:CDS 50.0%
GTTGAAGACAGTTCGTGCTG+AGG + chr5.3:21524193-21524212 MS.gene29137:CDS 50.0%
GTTTGACCGAAAGCAAGTGC+CGG - chr5.3:21523504-21523523 None:intergenic 50.0%
TAGTTTCTACGCTGAGCCTG+TGG - chr5.3:21523855-21523874 None:intergenic 50.0%
TGGTTCGAATGCAAGCCGAT+GGG + chr5.3:21523823-21523842 MS.gene29137:CDS 50.0%
TGTCGACGCCATTACCAGAA+TGG + chr5.3:21523884-21523903 MS.gene29137:CDS 50.0%
! GGTCCCATGGCTCTATACAA+GGG + chr5.3:21524215-21524234 MS.gene29137:CDS 50.0%
! TCCAAAGCACCAGCATAAGG+TGG - chr5.3:21524167-21524186 None:intergenic 50.0%
! TTTTGCAGCGGGATTTGTGG+CGG + chr5.3:21524070-21524089 MS.gene29137:CDS 50.0%
!! ATCGGCTTGCATTCGAACCA+TGG - chr5.3:21523823-21523842 None:intergenic 50.0%
!! CGTGTTGGCCTTATCTCAGT+CGG + chr5.3:21523570-21523589 MS.gene29137:CDS 50.0%
!! GAGAAGGGTGTTATGCGTGA+TGG + chr5.3:21524023-21524042 MS.gene29137:CDS 50.0%
!! GTGTTATGCGTGATGGGCTT+GGG + chr5.3:21524030-21524049 MS.gene29137:CDS 50.0%
!!! GTGACAGCTAGTTTTGCAGC+GGG + chr5.3:21524059-21524078 MS.gene29137:CDS 50.0%
AAACAGCGGCATAGTGCCAC+CGG - chr5.3:21523733-21523752 None:intergenic 55.0%
AAGCTGTGACCAACATGGCG+CGG - chr5.3:21523962-21523981 None:intergenic 55.0%
AAGTGGACGGATCGTGAAGC+CGG + chr5.3:21523711-21523730 MS.gene29137:CDS 55.0%
ACCAGAATGGCGAAGCAGGA+AGG + chr5.3:21523897-21523916 MS.gene29137:CDS 55.0%
ACCTTCCTGCTTCGCCATTC+TGG - chr5.3:21523901-21523920 None:intergenic 55.0%
ACTCTACTCCACCACCAGGA+TGG + chr5.3:21523665-21523684 MS.gene29137:CDS 55.0%
AGACACTCTACTCCACCACC+AGG + chr5.3:21523661-21523680 MS.gene29137:CDS 55.0%
AGTAGACCCCACATGGATCG+TGG - chr5.3:21523535-21523554 None:intergenic 55.0%
ATAAGGCCAACACGAGGCTG+AGG - chr5.3:21523564-21523583 None:intergenic 55.0%
ATCTGAACCGGGTTCGGCTT+AGG - chr5.3:21523474-21523493 None:intergenic 55.0%
CGGAACAACCACGATCCATG+TGG + chr5.3:21523524-21523543 MS.gene29137:CDS 55.0%
CTCTACTCCACCACCAGGAT+GGG + chr5.3:21523666-21523685 MS.gene29137:CDS 55.0%
CTGTTTCGCAAGATCGAGGC+TGG + chr5.3:21523747-21523766 MS.gene29137:CDS 55.0%
GATCCATGTGGGGTCTACTC+CGG + chr5.3:21523536-21523555 MS.gene29137:CDS 55.0%
GCCTCGATCTTGCGAAACAG+CGG - chr5.3:21523747-21523766 None:intergenic 55.0%
GTTGGTCACAGCTTCACAGC+TGG + chr5.3:21523968-21523987 MS.gene29137:CDS 55.0%
TCGAACCATGGCAACATCGG+CGG - chr5.3:21523811-21523830 None:intergenic 55.0%
TTCGTAGAGTCCCATCCTGG+TGG - chr5.3:21523679-21523698 None:intergenic 55.0%
! AGGAAGGTGTGACCAGTCTG+TGG + chr5.3:21523913-21523932 MS.gene29137:CDS 55.0%
! GACGGACTCCGACTGAGATA+AGG - chr5.3:21523581-21523600 None:intergenic 55.0%
! GGGTCCCATGGCTCTATACA+AGG + chr5.3:21524214-21524233 MS.gene29137:CDS 55.0%
!! GGTGTTATGCGTGATGGGCT+TGG + chr5.3:21524029-21524048 MS.gene29137:CDS 55.0%
AAACGCGCCTAAGCCGAACC+CGG + chr5.3:21523464-21523483 MS.gene29137:CDS 60.0%
AGCCTGTGGGAGTCTCCCAT+CGG - chr5.3:21523841-21523860 None:intergenic 60.0%
AGGCTGGACTTATTGCCGGC+GGG + chr5.3:21523763-21523782 MS.gene29137:CDS 60.0%
AGTTCGTGCTGAGGGTCCCA+TGG + chr5.3:21524202-21524221 MS.gene29137:CDS 60.0%
CCAACACGAGGCTGAGGAAC+CGG - chr5.3:21523558-21523577 None:intergenic 60.0%
CGAACCATGGCAACATCGGC+GGG - chr5.3:21523810-21523829 None:intergenic 60.0%
CGGAGGATCTGAACCGGGTT+CGG - chr5.3:21523480-21523499 None:intergenic 60.0%
CTCCGACCGGCACTTGCTTT+CGG + chr5.3:21523495-21523514 MS.gene29137:CDS 60.0%
GAACCATGGCAACATCGGCG+GGG - chr5.3:21523809-21523828 None:intergenic 60.0%
GAACCGGAGTAGACCCCACA+TGG - chr5.3:21523542-21523561 None:intergenic 60.0%
GACTGTGAACCGCGCCATGT+TGG + chr5.3:21523950-21523969 MS.gene29137:CDS 60.0%
GAGGCTGGACTTATTGCCGG+CGG + chr5.3:21523762-21523781 MS.gene29137:CDS 60.0%
GAGTGTCTGCCTGAGCACAG+TGG - chr5.3:21523649-21523668 None:intergenic 60.0%
GTAGAGTCCCATCCTGGTGG+TGG - chr5.3:21523676-21523695 None:intergenic 60.0%
TAACCCCGCCGATGTTGCCA+TGG + chr5.3:21523803-21523822 MS.gene29137:CDS 60.0%
TGTCTGCCTGAGCACAGTGG+CGG - chr5.3:21523646-21523665 None:intergenic 60.0%
! GCCGCTGTTTCGCAAGATCG+AGG + chr5.3:21523743-21523762 MS.gene29137:CDS 60.0%
!! GACCGAAAGCAAGTGCCGGT+CGG - chr5.3:21523500-21523519 None:intergenic 60.0%
AGCCGATGGGAGACTCCCAC+AGG + chr5.3:21523836-21523855 MS.gene29137:CDS 65.0%
CCCGGTTCAGATCCTCCGAC+CGG + chr5.3:21523482-21523501 MS.gene29137:CDS 65.0%
CCGGTCGGAGGATCTGAACC+GGG - chr5.3:21523485-21523504 None:intergenic 65.0%
CCGGTTCCTCAGCCTCGTGT+TGG + chr5.3:21523555-21523574 MS.gene29137:CDS 65.0%
GATAGCAGCACCAACCCCGC+CGG - chr5.3:21523781-21523800 None:intergenic 65.0%
GCATCTCCGCCACTGTGCTC+AGG + chr5.3:21523637-21523656 MS.gene29137:CDS 65.0%
GCCGGTCGGAGGATCTGAAC+CGG - chr5.3:21523486-21523505 None:intergenic 65.0%
GGACTTATTGCCGGCGGGGT+TGG + chr5.3:21523768-21523787 MS.gene29137:CDS 65.0%
GGCGGCTGTTGCGTCGAATC+CGG + chr5.3:21524088-21524107 MS.gene29137:CDS 65.0%
GGCTGGACTTATTGCCGGCG+GGG + chr5.3:21523764-21523783 MS.gene29137:CDS 65.0%
GGCTGTTGCGTCGAATCCGG+TGG + chr5.3:21524091-21524110 MS.gene29137:CDS 65.0%
GGTGTGACCAGTCTGTGGCG+CGG + chr5.3:21523918-21523937 MS.gene29137:CDS 65.0%
GTGTGACCAGTCTGTGGCGC+GGG + chr5.3:21523919-21523938 MS.gene29137:CDS 65.0%
TGGACGGATCGTGAAGCCGG+TGG + chr5.3:21523714-21523733 MS.gene29137:CDS 65.0%
! GAGCACAGTGGCGGAGATGC+CGG - chr5.3:21523637-21523656 None:intergenic 65.0%
!! CGAAAGCAAGTGCCGGTCGG+AGG - chr5.3:21523497-21523516 None:intergenic 65.0%
!! GGCGGGGTTGGTGCTGCTAT+CGG + chr5.3:21523780-21523799 MS.gene29137:CDS 65.0%
CGATGACCCGCGCCACAGAC+TGG - chr5.3:21523928-21523947 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 21523360 21524328 21523360 ID=MS.gene29137
chr5.3 mRNA 21523360 21524328 21523360 ID=MS.gene29137.t1;Parent=MS.gene29137
chr5.3 exon 21523360 21524328 21523360 ID=MS.gene29137.t1.exon1;Parent=MS.gene29137.t1
chr5.3 CDS 21523360 21524328 21523360 ID=cds.MS.gene29137.t1;Parent=MS.gene29137.t1
Gene Sequence

>MS.gene29137

ATGGGTGTCAAAGGTTTTGTTGAAGGTGGTATTGCTTCAATCATCGCAGGATGTTCCACACATCCACTAGATCTTATTAAGGTTCGCATGCAACTTCAAGGTGAAAACGCGCCTAAGCCGAACCCGGTTCAGATCCTCCGACCGGCACTTGCTTTCGGTCAAACCGGAACAACCACGATCCATGTGGGGTCTACTCCGGTTCCTCAGCCTCGTGTTGGCCTTATCTCAGTCGGAGTCCGTCTTGTTCAACAAGAAGGTGTAACAGCTTTGTTCTCCGGCATCTCCGCCACTGTGCTCAGGCAGACACTCTACTCCACCACCAGGATGGGACTCTACGAAGTTCTGAAAAACAAGTGGACGGATCGTGAAGCCGGTGGCACTATGCCGCTGTTTCGCAAGATCGAGGCTGGACTTATTGCCGGCGGGGTTGGTGCTGCTATCGGTAACCCCGCCGATGTTGCCATGGTTCGAATGCAAGCCGATGGGAGACTCCCACAGGCTCAGCGTAGAAACTACAAATCTGTTGTCGACGCCATTACCAGAATGGCGAAGCAGGAAGGTGTGACCAGTCTGTGGCGCGGGTCATCGTTGACTGTGAACCGCGCCATGTTGGTCACAGCTTCACAGCTGGCTTCATATGATCAATTCAAGGAGATGATTCTTGAGAAGGGTGTTATGCGTGATGGGCTTGGGACCCATGTGACAGCTAGTTTTGCAGCGGGATTTGTGGCGGCTGTTGCGTCGAATCCGGTGGATGTTATTAAGACAAGGGTGATGAACATGAAGGTGGAGGTTGGAAAGGAACCACCTTATGCTGGTGCTTTGGATTGTGCGTTGAAGACAGTTCGTGCTGAGGGTCCCATGGCTCTATACAAGGGATTTATTCCCACAATTTCTAGGCAGGGACCTTTCACTGTTGTTCTGTTTGTTACTTTGGAGCAAGTTCGCAAGTTGTTGAAGGATTTCTGA
Protein sequence

>MS.gene29137.t1

MGVKGFVEGGIASIIAGCSTHPLDLIKVRMQLQGENAPKPNPVQILRPALAFGQTGTTTIHVGSTPVPQPRVGLISVGVRLVQQEGVTALFSGISATVLRQTLYSTTRMGLYEVLKNKWTDREAGGTMPLFRKIEAGLIAGGVGAAIGNPADVAMVRMQADGRLPQAQRRNYKSVVDAITRMAKQEGVTSLWRGSSLTVNRAMLVTASQLASYDQFKEMILEKGVMRDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEVGKEPPYAGALDCALKTVRAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKLLKDF