Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29309.t1 | XP_003626325.2 | 90.3 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 7.00E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29309.t1 | G7KZQ0 | 90.3 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 5.1e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049341 | MS.gene29309 | 0.812813 | 3.51E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene29309 | 0.80038 | 1.48E-48 | -1.69E-46 |
| MS.gene050020 | MS.gene29309 | 0.837665 | 4.55E-57 | -1.69E-46 |
| MS.gene050438 | MS.gene29309 | 0.804004 | 2.66E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene29309 | 0.813671 | 2.28E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene29309 | 0.81436 | 1.60E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene29309 | 0.834321 | 3.21E-56 | -1.69E-46 |
| MS.gene051720 | MS.gene29309 | 0.814653 | 1.38E-51 | -1.69E-46 |
| MS.gene052020 | MS.gene29309 | 0.822293 | 2.56E-53 | -1.69E-46 |
| MS.gene052323 | MS.gene29309 | 0.811098 | 8.30E-51 | -1.69E-46 |
| MS.gene052611 | MS.gene29309 | 0.80195 | 7.07E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene29309 | 0.827596 | 1.43E-54 | -1.69E-46 |
| MS.gene052896 | MS.gene29309 | 0.803932 | 2.75E-49 | -1.69E-46 |
| MS.gene053221 | MS.gene29309 | 0.810687 | 1.02E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene29309 | 0.802911 | 4.48E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene29309 | 0.805483 | 1.31E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene29309 | 0.806189 | 9.28E-50 | -1.69E-46 |
| MS.gene058636 | MS.gene29309 | 0.810121 | 1.35E-50 | -1.69E-46 |
| MS.gene059937 | MS.gene29309 | 0.827984 | 1.16E-54 | -1.69E-46 |
| MS.gene060587 | MS.gene29309 | 0.824884 | 6.34E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29309.t1 | MTR_7g113900 | 90.345 | 145 | 10 | 2 | 1 | 145 | 24 | 164 | 3.34e-86 | 249 |
| MS.gene29309.t1 | MTR_2g024090 | 40.132 | 152 | 81 | 3 | 1 | 142 | 16 | 167 | 1.38e-29 | 106 |
| MS.gene29309.t1 | MTR_4g050050 | 35.625 | 160 | 86 | 2 | 1 | 143 | 18 | 177 | 1.74e-28 | 103 |
| MS.gene29309.t1 | MTR_4g050050 | 36.364 | 143 | 75 | 2 | 1 | 143 | 18 | 144 | 4.62e-27 | 99.4 |
| MS.gene29309.t1 | MTR_2g450100 | 42.982 | 114 | 62 | 1 | 33 | 143 | 63 | 176 | 1.42e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29309.t1 | AT1G05070 | 41.060 | 151 | 70 | 5 | 8 | 143 | 22 | 168 | 1.23e-31 | 111 |
| MS.gene29309.t1 | AT2G32580 | 37.662 | 154 | 84 | 3 | 1 | 143 | 15 | 167 | 4.85e-30 | 107 |
| MS.gene29309.t1 | AT4G04360 | 41.935 | 155 | 76 | 4 | 1 | 143 | 16 | 168 | 1.45e-28 | 103 |
| MS.gene29309.t1 | AT2G24290 | 39.873 | 158 | 80 | 3 | 1 | 143 | 15 | 172 | 2.74e-28 | 102 |
| MS.gene29309.t1 | AT4G30996 | 50.515 | 97 | 48 | 0 | 45 | 141 | 73 | 169 | 1.41e-27 | 100 |
| MS.gene29309.t1 | AT2G32580 | 43.000 | 100 | 57 | 0 | 44 | 143 | 6 | 105 | 1.58e-24 | 91.7 |
Find 40 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATATCCCGTTGCTTCTT+TGG | 0.212587 | 7.2:+3449570 | MS.gene29309:CDS |
| TGCATTTATTACTAATCTTT+TGG | 0.212992 | 7.2:-3450380 | None:intergenic |
| GAAGAACGAGCTCGTGATTA+TGG | 0.234749 | 7.2:+3450529 | MS.gene29309:CDS |
| TTCATTTCCTCCTTCATTGT+TGG | 0.237361 | 7.2:-3450283 | None:intergenic |
| AGCGCAGGTTGACGGCATTA+TGG | 0.273292 | 7.2:+3450506 | MS.gene29309:CDS |
| CTGTTCCTCACTTGCCTCAC+TGG | 0.367272 | 7.2:+3449549 | MS.gene29309:CDS |
| TGTTACCTTGTGTTCACAAT+TGG | 0.371180 | 7.2:+3449469 | MS.gene29309:CDS |
| GAATATCCCGTTGCTTCTTT+GGG | 0.376906 | 7.2:+3449571 | MS.gene29309:CDS |
| GCGCAGGTTGACGGCATTAT+GGG | 0.391190 | 7.2:+3450507 | MS.gene29309:CDS |
| TTACTATATGTACAGATTGT+GGG | 0.418392 | 7.2:+3450251 | MS.gene29309:intron |
| GAAGTAGAAAAGTGCAGCAT+AGG | 0.426789 | 7.2:+3450430 | MS.gene29309:CDS |
| CAGAGCTTGTTGAAGAGCGC+AGG | 0.429709 | 7.2:+3450491 | MS.gene29309:CDS |
| AAGAACGAGCTCGTGATTAT+GGG | 0.441049 | 7.2:+3450530 | MS.gene29309:CDS |
| GGTCTGTTCCAAAGTCTCAT+TGG | 0.443502 | 7.2:-3450359 | None:intergenic |
| AATTGTAGCCAATGAGACTT+TGG | 0.447499 | 7.2:+3450351 | MS.gene29309:CDS |
| GTGATTATGGGTGGAAGGAC+AGG | 0.457853 | 7.2:+3450542 | MS.gene29309:CDS |
| AACTTACCCAAAGAAGCAAC+GGG | 0.499220 | 7.2:-3449577 | None:intergenic |
| TGTTGAAGAGCGCAGGTTGA+CGG | 0.500440 | 7.2:+3450498 | MS.gene29309:CDS |
| AAGTAGAAAAGTGCAGCATA+GGG | 0.518644 | 7.2:+3450431 | MS.gene29309:CDS |
| TTCTTCAAACGCCAGCGAGA+TGG | 0.541960 | 7.2:-3449496 | None:intergenic |
| AGAAAAGTGCAGCATAGGGA+TGG | 0.556983 | 7.2:+3450435 | MS.gene29309:CDS |
| CAATCATGACATTGAGGACA+AGG | 0.561176 | 7.2:-3449523 | None:intergenic |
| CAATTGGACCACCATCTCGC+TGG | 0.563845 | 7.2:+3449485 | MS.gene29309:CDS |
| TGGAAACTTGTGAAGAAGCA+AGG | 0.573605 | 7.2:+3450455 | MS.gene29309:CDS |
| TTCAAACGCCAGCGAGATGG+TGG | 0.580007 | 7.2:-3449493 | None:intergenic |
| AGAAGCAAGGGAGAGAGCAG+AGG | 0.585238 | 7.2:+3450468 | MS.gene29309:CDS |
| GAGACATGATCCAACAATGA+AGG | 0.590741 | 7.2:+3450273 | MS.gene29309:CDS |
| AGCTCGTGATTATGGGTGGA+AGG | 0.596678 | 7.2:+3450537 | MS.gene29309:CDS |
| AAAGATTAGTAATAAATGCA+AGG | 0.596837 | 7.2:+3450383 | MS.gene29309:CDS |
| TCCTCTGATAGCATTGTCAG+TGG | 0.601328 | 7.2:-3450313 | None:intergenic |
| AAACTTACCCAAAGAAGCAA+CGG | 0.604283 | 7.2:-3449578 | None:intergenic |
| AACGAGCTCGTGATTATGGG+TGG | 0.612321 | 7.2:+3450533 | MS.gene29309:CDS |
| GGAAACTTGTGAAGAAGCAA+GGG | 0.615984 | 7.2:+3450456 | MS.gene29309:CDS |
| CAGTAACAATCATGACATTG+AGG | 0.634830 | 7.2:-3449529 | None:intergenic |
| TCCACTGACAATGCTATCAG+AGG | 0.642154 | 7.2:+3450312 | MS.gene29309:CDS |
| ATATTCCAGTGAGGCAAGTG+AGG | 0.642772 | 7.2:-3449554 | None:intergenic |
| ACATGATCCAACAATGAAGG+AGG | 0.647322 | 7.2:+3450276 | MS.gene29309:CDS |
| GTGGTCCAATTGTGAACACA+AGG | 0.658093 | 7.2:-3449474 | None:intergenic |
| AATGAAGGAGGAAATGAACA+AGG | 0.667487 | 7.2:+3450288 | MS.gene29309:CDS |
| AGCAACGGGATATTCCAGTG+AGG | 0.784097 | 7.2:-3449563 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATAAATTTTACTTAAAG+TGG | - | chr7.2:3450109-3450128 | None:intergenic | 10.0% |
| !! | AAAGATTAGTAATAAATGCA+AGG | + | chr7.2:3450383-3450402 | MS.gene29309:CDS | 20.0% |
| !! | AGAAATGCAAAACATTTAAA+TGG | - | chr7.2:3450020-3450039 | None:intergenic | 20.0% |
| !! | CAATTCAATTCATATGTATT+AGG | + | chr7.2:3449800-3449819 | MS.gene29309:intron | 20.0% |
| !! | TAGGTTTATATATTAATTCG+TGG | + | chr7.2:3449819-3449838 | MS.gene29309:intron | 20.0% |
| !! | TAGTAATTAGAAAAATTCAC+TGG | + | chr7.2:3449713-3449732 | MS.gene29309:intron | 20.0% |
| !!! | TGCATTTATTACTAATCTTT+TGG | - | chr7.2:3450383-3450402 | None:intergenic | 20.0% |
| ! | ATTGAATAACCAAAACATTC+TGG | - | chr7.2:3449784-3449803 | None:intergenic | 25.0% |
| ! | ATTTGTAGAGTAAGTGAAAA+AGG | - | chr7.2:3449605-3449624 | None:intergenic | 25.0% |
| ! | TTACTATATGTACAGATTGT+GGG | + | chr7.2:3450251-3450270 | MS.gene29309:intron | 25.0% |
| ! | TTTACTATATGTACAGATTG+TGG | + | chr7.2:3450250-3450269 | MS.gene29309:intron | 25.0% |
| ! | TTTGTAGAGTAAGTGAAAAA+GGG | - | chr7.2:3449604-3449623 | None:intergenic | 25.0% |
| !!! | CATCATTTTTCAAACACAAA+GGG | - | chr7.2:3449690-3449709 | None:intergenic | 25.0% |
| ACAAGTTTGATTAGATGTAC+AGG | + | chr7.2:3449653-3449672 | MS.gene29309:intron | 30.0% | |
| ACTCTACAAATTAACAGACT+TGG | + | chr7.2:3449614-3449633 | MS.gene29309:intron | 30.0% | |
| ATAATGTCCAAAAGACAACA+TGG | - | chr7.2:3450212-3450231 | None:intergenic | 30.0% | |
| CTAAAAATGAGAACAGAATG+AGG | - | chr7.2:3450169-3450188 | None:intergenic | 30.0% | |
| CTTTGTTCTGATTCTCAAAA+AGG | - | chr7.2:3449742-3449761 | None:intergenic | 30.0% | |
| TGATACAAAACATACATCGA+AGG | + | chr7.2:3449913-3449932 | MS.gene29309:intron | 30.0% | |
| ! | ATGTATGTTTTGTATCACAG+TGG | - | chr7.2:3449910-3449929 | None:intergenic | 30.0% |
| !! | GCATCATTTTTCAAACACAA+AGG | - | chr7.2:3449691-3449710 | None:intergenic | 30.0% |
| AAACTTACCCAAAGAAGCAA+CGG | - | chr7.2:3449581-3449600 | None:intergenic | 35.0% | |
| AATGAAGGAGGAAATGAACA+AGG | + | chr7.2:3450288-3450307 | MS.gene29309:CDS | 35.0% | |
| AATTGTAGCCAATGAGACTT+TGG | + | chr7.2:3450351-3450370 | MS.gene29309:CDS | 35.0% | |
| CAGTAACAATCATGACATTG+AGG | - | chr7.2:3449532-3449551 | None:intergenic | 35.0% | |
| TGTTACCTTGTGTTCACAAT+TGG | + | chr7.2:3449469-3449488 | MS.gene29309:CDS | 35.0% | |
| TTCATTTCCTCCTTCATTGT+TGG | - | chr7.2:3450286-3450305 | None:intergenic | 35.0% | |
| ! | AAGTAGAAAAGTGCAGCATA+GGG | + | chr7.2:3450431-3450450 | MS.gene29309:CDS | 35.0% |
| ! | TCTATCTAGCCAGAATGTTT+TGG | + | chr7.2:3449772-3449791 | MS.gene29309:intron | 35.0% |
| !! | CATAGCTAGCTTTGTTTATG+TGG | + | chr7.2:3450045-3450064 | MS.gene29309:intron | 35.0% |
| AACTTACCCAAAGAAGCAAC+GGG | - | chr7.2:3449580-3449599 | None:intergenic | 40.0% | |
| AAGAACGAGCTCGTGATTAT+GGG | + | chr7.2:3450530-3450549 | MS.gene29309:CDS | 40.0% | |
| ACATGATCCAACAATGAAGG+AGG | + | chr7.2:3450276-3450295 | MS.gene29309:CDS | 40.0% | |
| ATGTCCAAAAGACAACATGG+TGG | - | chr7.2:3450209-3450228 | None:intergenic | 40.0% | |
| CAATCATGACATTGAGGACA+AGG | - | chr7.2:3449526-3449545 | None:intergenic | 40.0% | |
| GAATATCCCGTTGCTTCTTT+GGG | + | chr7.2:3449571-3449590 | MS.gene29309:CDS | 40.0% | |
| GAGACATGATCCAACAATGA+AGG | + | chr7.2:3450273-3450292 | MS.gene29309:CDS | 40.0% | |
| GGAAACTTGTGAAGAAGCAA+GGG | + | chr7.2:3450456-3450475 | MS.gene29309:CDS | 40.0% | |
| TGGAAACTTGTGAAGAAGCA+AGG | + | chr7.2:3450455-3450474 | MS.gene29309:CDS | 40.0% | |
| ! | GAAGTAGAAAAGTGCAGCAT+AGG | + | chr7.2:3450430-3450449 | MS.gene29309:CDS | 40.0% |
| ! | TTCACCACCATGTTGTCTTT+TGG | + | chr7.2:3450202-3450221 | MS.gene29309:intron | 40.0% |
| ! | TTGTTTAGCTCAACACGGTA+CGG | + | chr7.2:3449943-3449962 | MS.gene29309:intron | 40.0% |
| !! | TTGCCTTGTTTAGCTCAACA+CGG | + | chr7.2:3449938-3449957 | MS.gene29309:intron | 40.0% |
| ATATTCCAGTGAGGCAAGTG+AGG | - | chr7.2:3449557-3449576 | None:intergenic | 45.0% | |
| GAAGAACGAGCTCGTGATTA+TGG | + | chr7.2:3450529-3450548 | MS.gene29309:CDS | 45.0% | |
| GGAATATCCCGTTGCTTCTT+TGG | + | chr7.2:3449570-3449589 | MS.gene29309:CDS | 45.0% | |
| GGTCTGTTCCAAAGTCTCAT+TGG | - | chr7.2:3450362-3450381 | None:intergenic | 45.0% | |
| GTACCGTGTTGAGCTAAACA+AGG | - | chr7.2:3449944-3449963 | None:intergenic | 45.0% | |
| GTGGTCCAATTGTGAACACA+AGG | - | chr7.2:3449477-3449496 | None:intergenic | 45.0% | |
| TCCACTGACAATGCTATCAG+AGG | + | chr7.2:3450312-3450331 | MS.gene29309:CDS | 45.0% | |
| TCCTCTGATAGCATTGTCAG+TGG | - | chr7.2:3450316-3450335 | None:intergenic | 45.0% | |
| ! | AGAAAAGTGCAGCATAGGGA+TGG | + | chr7.2:3450435-3450454 | MS.gene29309:CDS | 45.0% |
| ! | TGTTTAGCTCAACACGGTAC+GGG | + | chr7.2:3449944-3449963 | MS.gene29309:intron | 45.0% |
| AACGAGCTCGTGATTATGGG+TGG | + | chr7.2:3450533-3450552 | MS.gene29309:CDS | 50.0% | |
| AGCAACGGGATATTCCAGTG+AGG | - | chr7.2:3449566-3449585 | None:intergenic | 50.0% | |
| AGCTCGTGATTATGGGTGGA+AGG | + | chr7.2:3450537-3450556 | MS.gene29309:CDS | 50.0% | |
| GTGATTATGGGTGGAAGGAC+AGG | + | chr7.2:3450542-3450561 | MS.gene29309:CDS | 50.0% | |
| TTCTTCAAACGCCAGCGAGA+TGG | - | chr7.2:3449499-3449518 | None:intergenic | 50.0% | |
| ! | GTTTAGCTCAACACGGTACG+GGG | + | chr7.2:3449945-3449964 | MS.gene29309:intron | 50.0% |
| ! | TGTTGAAGAGCGCAGGTTGA+CGG | + | chr7.2:3450498-3450517 | MS.gene29309:CDS | 50.0% |
| AGAAGCAAGGGAGAGAGCAG+AGG | + | chr7.2:3450468-3450487 | MS.gene29309:CDS | 55.0% | |
| CAATTGGACCACCATCTCGC+TGG | + | chr7.2:3449485-3449504 | MS.gene29309:CDS | 55.0% | |
| CTGTTCCTCACTTGCCTCAC+TGG | + | chr7.2:3449549-3449568 | MS.gene29309:CDS | 55.0% | |
| TTCAAACGCCAGCGAGATGG+TGG | - | chr7.2:3449496-3449515 | None:intergenic | 55.0% | |
| ! | AGCGCAGGTTGACGGCATTA+TGG | + | chr7.2:3450506-3450525 | MS.gene29309:CDS | 55.0% |
| ! | GCGCAGGTTGACGGCATTAT+GGG | + | chr7.2:3450507-3450526 | MS.gene29309:CDS | 55.0% |
| !! | CAGAGCTTGTTGAAGAGCGC+AGG | + | chr7.2:3450491-3450510 | MS.gene29309:CDS | 55.0% |
| GCTCAACACGGTACGGGGAT+TGG | + | chr7.2:3449950-3449969 | MS.gene29309:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 3449460 | 3450570 | 3449460 | ID=MS.gene29309 |
| chr7.2 | mRNA | 3449460 | 3450570 | 3449460 | ID=MS.gene29309.t1;Parent=MS.gene29309 |
| chr7.2 | exon | 3449460 | 3449592 | 3449460 | ID=MS.gene29309.t1.exon1;Parent=MS.gene29309.t1 |
| chr7.2 | CDS | 3449460 | 3449592 | 3449460 | ID=cds.MS.gene29309.t1;Parent=MS.gene29309.t1 |
| chr7.2 | exon | 3450266 | 3450570 | 3450266 | ID=MS.gene29309.t1.exon2;Parent=MS.gene29309.t1 |
| chr7.2 | CDS | 3450266 | 3450570 | 3450266 | ID=cds.MS.gene29309.t1;Parent=MS.gene29309.t1 |
| Gene Sequence |
| Protein sequence |
