Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049979.t1 | XP_003615310.2 | 97.8 | 90 | 2 | 0 | 1 | 90 | 505 | 594 | 8.30E-43 | 182.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049979.t1 | Q9SUM4 | 52.9 | 70 | 33 | 0 | 15 | 84 | 619 | 688 | 3.7e-15 | 82.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049979.t1 | G7JYE8 | 97.8 | 90 | 2 | 0 | 1 | 90 | 499 | 588 | 6.0e-43 | 182.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049979 | MS.gene050020 | 0.817301 | 3.54E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene050807 | 0.805255 | 1.46E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene050815 | 0.8077 | 4.45E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene052020 | 0.802084 | 6.64E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene052611 | 0.804804 | 1.81E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene052665 | 0.818112 | 2.32E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene055512 | 0.804699 | 1.91E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene058906 | 0.810175 | 1.31E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene058982 | 0.812218 | 4.74E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene059937 | 0.814161 | 1.77E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene061618 | 0.818084 | 2.36E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene061893 | 0.806399 | 8.38E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene061947 | 0.823606 | 1.27E-53 | -1.69E-46 |
| MS.gene049979 | MS.gene062352 | 0.806663 | 7.37E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene06236 | 0.809229 | 2.10E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene064944 | 0.831824 | 1.34E-55 | -1.69E-46 |
| MS.gene049979 | MS.gene065392 | 0.800831 | 1.20E-48 | -1.69E-46 |
| MS.gene049979 | MS.gene065825 | 0.814461 | 1.52E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene066112 | 0.822238 | 2.64E-53 | -1.69E-46 |
| MS.gene049979 | MS.gene067071 | 0.823196 | 1.58E-53 | -1.69E-46 |
| MS.gene049979 | MS.gene068659 | 0.804224 | 2.39E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene070943 | 0.827586 | 1.44E-54 | -1.69E-46 |
| MS.gene049979 | MS.gene071315 | 0.801369 | 9.30E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene071316 | 0.800221 | 1.60E-48 | -1.69E-46 |
| MS.gene049979 | MS.gene07147 | 0.817693 | 2.89E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene072442 | 0.804821 | 1.80E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene073085 | 0.801233 | 9.92E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene07530 | 0.804687 | 1.92E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene08428 | 0.800875 | 1.17E-48 | -1.69E-46 |
| MS.gene049979 | MS.gene20375 | 0.803983 | 2.69E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene23105 | 0.806762 | 7.03E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene24268 | 0.814726 | 1.33E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene24610 | 0.801401 | 9.16E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene25810 | 0.801803 | 7.58E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene26972 | 0.826278 | 2.96E-54 | -1.69E-46 |
| MS.gene049979 | MS.gene27918 | 0.815175 | 1.06E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene27991 | 0.816775 | 4.65E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene28658 | 0.806875 | 6.65E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene28854 | 0.810771 | 9.76E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene29309 | 0.80038 | 1.48E-48 | -1.69E-46 |
| MS.gene049979 | MS.gene29576 | 0.802295 | 6.00E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene34369 | 0.805768 | 1.14E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene36281 | 0.806964 | 6.37E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene41679 | 0.824432 | 8.10E-54 | -1.69E-46 |
| MS.gene049979 | MS.gene42162 | 0.805684 | 1.19E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene47689 | 0.810746 | 9.89E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene47691 | 0.808063 | 3.72E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene47875 | 0.817508 | 3.18E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene47955 | 0.81479 | 1.29E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene48321 | 0.824489 | 7.85E-54 | -1.69E-46 |
| MS.gene049979 | MS.gene50990 | 0.81288 | 3.39E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene51240 | 0.810097 | 1.37E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene53586 | 0.829464 | 5.07E-55 | -1.69E-46 |
| MS.gene049979 | MS.gene54919 | 0.809498 | 1.84E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene56555 | 0.805899 | 1.07E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene57388 | 0.818415 | 1.98E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene59838 | 0.801748 | 7.78E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene62682 | 0.822115 | 2.82E-53 | -1.69E-46 |
| MS.gene049979 | MS.gene62720 | 0.814659 | 1.38E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene64101 | 0.805071 | 1.59E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene65276 | 0.828729 | 7.65E-55 | -1.69E-46 |
| MS.gene049979 | MS.gene66280 | 0.805438 | 1.33E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene70730 | 0.808723 | 2.69E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene72141 | 0.803005 | 4.28E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene72268 | 0.80068 | 1.29E-48 | -1.69E-46 |
| MS.gene049979 | MS.gene72812 | 0.808586 | 2.88E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene74226 | 0.824908 | 6.25E-54 | -1.69E-46 |
| MS.gene049979 | MS.gene74980 | 0.830311 | 3.15E-55 | -1.69E-46 |
| MS.gene049979 | MS.gene76734 | 0.801572 | 8.45E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene78337 | 0.807975 | 3.89E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene81998 | 0.803332 | 3.67E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene82119 | 0.802199 | 6.28E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene85604 | 0.819395 | 1.19E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene85606 | 0.812081 | 5.07E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene86678 | 0.81597 | 7.04E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene88087 | 0.807531 | 4.83E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene89096 | 0.804402 | 2.20E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene90649 | 0.817959 | 2.52E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene90689 | 0.81599 | 6.96E-52 | -1.69E-46 |
| MS.gene049979 | MS.gene90787 | 0.81148 | 6.85E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene94406 | 0.821269 | 4.42E-53 | -1.69E-46 |
| MS.gene049979 | MS.gene95236 | 0.802754 | 4.83E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene95800 | 0.801103 | 1.05E-48 | -1.69E-46 |
| MS.gene049979 | MS.gene97761 | 0.813071 | 3.08E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene97762 | 0.800203 | 1.61E-48 | -1.69E-46 |
| MS.gene049979 | MS.gene98717 | 0.802336 | 5.89E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene99887 | 0.808044 | 3.76E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049979.t1 | MTR_5g066440 | 97.778 | 90 | 2 | 0 | 1 | 90 | 499 | 588 | 2.05e-58 | 188 |
| MS.gene049979.t1 | MTR_6g085190 | 55.714 | 70 | 31 | 0 | 15 | 84 | 553 | 622 | 3.84e-23 | 92.0 |
| MS.gene049979.t1 | MTR_4g127880 | 50.000 | 68 | 34 | 0 | 15 | 82 | 441 | 508 | 4.20e-19 | 80.5 |
| MS.gene049979.t1 | MTR_4g127880 | 50.000 | 68 | 34 | 0 | 15 | 82 | 624 | 691 | 4.50e-19 | 80.5 |
| MS.gene049979.t1 | MTR_4g127880 | 50.000 | 68 | 34 | 0 | 15 | 82 | 644 | 711 | 4.64e-19 | 80.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049979.t1 | AT4G30200 | 52.857 | 70 | 33 | 0 | 15 | 84 | 607 | 676 | 5.81e-20 | 82.8 |
| MS.gene049979.t1 | AT4G30200 | 52.857 | 70 | 33 | 0 | 15 | 84 | 590 | 659 | 6.03e-20 | 82.8 |
| MS.gene049979.t1 | AT4G30200 | 52.857 | 70 | 33 | 0 | 15 | 84 | 619 | 688 | 6.05e-20 | 82.8 |
| MS.gene049979.t1 | AT3G24440 | 56.250 | 64 | 28 | 0 | 19 | 82 | 522 | 585 | 1.52e-19 | 81.6 |
| MS.gene049979.t1 | AT3G24440 | 56.250 | 64 | 28 | 0 | 19 | 82 | 524 | 587 | 1.54e-19 | 81.6 |
| MS.gene049979.t1 | AT2G18870 | 48.485 | 66 | 34 | 0 | 19 | 84 | 145 | 210 | 3.53e-16 | 70.9 |
| MS.gene049979.t1 | AT5G57380 | 39.759 | 83 | 50 | 0 | 8 | 90 | 523 | 605 | 9.76e-16 | 70.9 |
| MS.gene049979.t1 | AT5G57380 | 39.759 | 83 | 50 | 0 | 8 | 90 | 503 | 585 | 1.03e-15 | 70.9 |
| MS.gene049979.t1 | AT2G18880 | 46.269 | 67 | 36 | 0 | 18 | 84 | 440 | 506 | 2.80e-13 | 63.9 |
Find 15 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGGTCTGCTAAGCATGC+AGG | 0.438648 | 5.3:-52177085 | None:intergenic |
| CAGTGAAAGTGTGCATTAGC+TGG | 0.492511 | 5.3:-52177102 | None:intergenic |
| TCAGAGTGAAGTTTCTCACT+TGG | 0.493449 | 5.3:+52176993 | MS.gene049979:CDS |
| TCTCACTTGGTTCAGTCTCA+AGG | 0.500305 | 5.3:+52177006 | MS.gene049979:CDS |
| GGCTAATCAGCAAGAGAGAA+GGG | 0.512174 | 5.3:+52177027 | MS.gene049979:CDS |
| ATTCTGTGAGGGTAGTCAAA+TGG | 0.527017 | 5.3:+52176939 | MS.gene049979:CDS |
| AGGCTAATCAGCAAGAGAGA+AGG | 0.548852 | 5.3:+52177026 | MS.gene049979:CDS |
| GTCAAATGGCTAGAGCATCA+AGG | 0.566998 | 5.3:+52176953 | MS.gene049979:CDS |
| GCTGGTCTGCTAAGCATGCA+GGG | 0.576398 | 5.3:-52177084 | None:intergenic |
| TCAAATGGCTAGAGCATCAA+GGG | 0.588860 | 5.3:+52176954 | MS.gene049979:CDS |
| AACGAAATGAGACAAATGAC+TGG | 0.614089 | 5.3:+52176875 | None:intergenic |
| TGATTATGAGTATTCTGTGA+GGG | 0.621336 | 5.3:+52176928 | MS.gene049979:CDS |
| GAATTCTAGAAAGCGCGTGA+AGG | 0.626345 | 5.3:+52176901 | MS.gene049979:CDS |
| ATGATTATGAGTATTCTGTG+AGG | 0.692962 | 5.3:+52176927 | MS.gene049979:CDS |
| TAATCAGCAAGAGAGAAGGG+TGG | 0.716441 | 5.3:+52177030 | MS.gene049979:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGATTATGAGTATTCTGTG+AGG | + | chr5.3:52176927-52176946 | MS.gene049979:CDS | 30.0% | |
| TGATTATGAGTATTCTGTGA+GGG | + | chr5.3:52176928-52176947 | MS.gene049979:CDS | 30.0% | |
| ATTCTGTGAGGGTAGTCAAA+TGG | + | chr5.3:52176939-52176958 | MS.gene049979:CDS | 40.0% | |
| TCAGAGTGAAGTTTCTCACT+TGG | + | chr5.3:52176993-52177012 | MS.gene049979:CDS | 40.0% | |
| ! | TCAAATGGCTAGAGCATCAA+GGG | + | chr5.3:52176954-52176973 | MS.gene049979:CDS | 40.0% |
| AGGCTAATCAGCAAGAGAGA+AGG | + | chr5.3:52177026-52177045 | MS.gene049979:CDS | 45.0% | |
| GGCTAATCAGCAAGAGAGAA+GGG | + | chr5.3:52177027-52177046 | MS.gene049979:CDS | 45.0% | |
| TAATCAGCAAGAGAGAAGGG+TGG | + | chr5.3:52177030-52177049 | MS.gene049979:CDS | 45.0% | |
| TCTCACTTGGTTCAGTCTCA+AGG | + | chr5.3:52177006-52177025 | MS.gene049979:CDS | 45.0% | |
| ! | CAGTGAAAGTGTGCATTAGC+TGG | - | chr5.3:52177105-52177124 | None:intergenic | 45.0% |
| ! | GAATTCTAGAAAGCGCGTGA+AGG | + | chr5.3:52176901-52176920 | MS.gene049979:CDS | 45.0% |
| ! | GTCAAATGGCTAGAGCATCA+AGG | + | chr5.3:52176953-52176972 | MS.gene049979:CDS | 45.0% |
| ! | AGCTGGTCTGCTAAGCATGC+AGG | - | chr5.3:52177088-52177107 | None:intergenic | 55.0% |
| ! | GCTGGTCTGCTAAGCATGCA+GGG | - | chr5.3:52177087-52177106 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 52176881 | 52177153 | 52176881 | ID=MS.gene049979 |
| chr5.3 | mRNA | 52176881 | 52177153 | 52176881 | ID=MS.gene049979.t1;Parent=MS.gene049979 |
| chr5.3 | exon | 52176881 | 52177153 | 52176881 | ID=MS.gene049979.t1.exon1;Parent=MS.gene049979.t1 |
| chr5.3 | CDS | 52176881 | 52177153 | 52176881 | ID=cds.MS.gene049979.t1;Parent=MS.gene049979.t1 |
| Gene Sequence |
| Protein sequence |
