Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29524.t1 | XP_024641057.1 | 73 | 274 | 53 | 3 | 1 | 272 | 1 | 255 | 3.70E-86 | 328.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29524.t1 | Q8W487 | 43.8 | 210 | 97 | 4 | 63 | 271 | 60 | 249 | 4.8e-26 | 119.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29524.t1 | A0A396I021 | 73.0 | 274 | 53 | 3 | 1 | 272 | 1 | 255 | 2.7e-86 | 328.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene055201 | MS.gene29524 | PPI |
| MS.gene29524 | MS.gene46105 | PPI |
| MS.gene29524 | MS.gene46104 | PPI |
| MS.gene29524 | MS.gene040272 | PPI |
| MS.gene29524 | MS.gene89319 | PPI |
| MS.gene29524 | MS.gene040271 | PPI |
| MS.gene29524 | MS.gene67544 | PPI |
| MS.gene000604 | MS.gene29524 | PPI |
| MS.gene29524 | MS.gene034255 | PPI |
| MS.gene29524 | MS.gene053230 | PPI |
| MS.gene29524 | MS.gene040269 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29524.t1 | MTR_5g080880 | 74.088 | 274 | 48 | 4 | 1 | 271 | 1 | 254 | 2.31e-125 | 360 |
| MS.gene29524.t1 | MTR_5g080870 | 63.603 | 272 | 47 | 4 | 1 | 272 | 1 | 220 | 3.38e-108 | 317 |
| MS.gene29524.t1 | MTR_7g063520 | 38.202 | 267 | 129 | 7 | 8 | 271 | 20 | 253 | 1.97e-45 | 153 |
| MS.gene29524.t1 | MTR_4g006560 | 31.515 | 165 | 89 | 3 | 108 | 271 | 94 | 235 | 8.54e-16 | 75.1 |
| MS.gene29524.t1 | MTR_1g013120 | 26.641 | 259 | 155 | 9 | 21 | 271 | 1 | 232 | 4.46e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29524.t1 | AT3G55605 | 40.206 | 291 | 120 | 12 | 1 | 271 | 1 | 257 | 3.53e-45 | 153 |
| MS.gene29524.t1 | AT5G05990 | 36.559 | 279 | 142 | 7 | 5 | 271 | 3 | 258 | 5.35e-44 | 150 |
| MS.gene29524.t1 | AT2G39795 | 40.476 | 210 | 104 | 5 | 63 | 271 | 60 | 249 | 7.95e-37 | 131 |
| MS.gene29524.t1 | AT5G02050 | 37.118 | 229 | 110 | 6 | 55 | 271 | 60 | 266 | 5.06e-35 | 127 |
| MS.gene29524.t1 | AT2G39790 | 28.968 | 252 | 139 | 7 | 31 | 272 | 19 | 240 | 4.96e-19 | 84.0 |
| MS.gene29524.t1 | AT1G15870 | 27.451 | 204 | 121 | 6 | 69 | 271 | 63 | 240 | 3.75e-12 | 65.1 |
Find 44 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATCAACAACTATCTTAAA+TGG | 0.169759 | 5.2:-68677894 | None:intergenic |
| TGCAGTTCAGATTGTTGTTT+TGG | 0.284237 | 5.2:+68678434 | MS.gene29524:CDS |
| TTATCAAGGTGAAGAAATTC+AGG | 0.325526 | 5.2:+68677947 | MS.gene29524:CDS |
| TAAGAAGGATGGGACCTCTT+TGG | 0.326135 | 5.2:+68678091 | MS.gene29524:CDS |
| TGCAAGAAATTTGTTGTGCT+TGG | 0.355860 | 5.2:-68678608 | None:intergenic |
| CAAGCATTCCACTTTCAGTC+AGG | 0.380172 | 5.2:+68678063 | MS.gene29524:CDS |
| CTGCGGATAACCATCTCATC+AGG | 0.394847 | 5.2:-68678134 | None:intergenic |
| CTCTTATTTCTAAGTACTTG+TGG | 0.408675 | 5.2:-68678577 | None:intergenic |
| CCAAGTCAATCACTTTCGTT+AGG | 0.411187 | 5.2:+68677407 | MS.gene29524:CDS |
| TTCAGTCAGGGTTTCTAAGA+AGG | 0.411975 | 5.2:+68678076 | MS.gene29524:CDS |
| TGCAAGAGTACATGATCAAC+TGG | 0.419125 | 5.2:+68678627 | MS.gene29524:CDS |
| CTGGAAAAGTAGAGAATACT+TGG | 0.432278 | 5.2:+68678646 | MS.gene29524:CDS |
| CACAAGTACTTAGAAATAAG+AGG | 0.434165 | 5.2:+68678578 | MS.gene29524:CDS |
| AACAACTTTGTTATTATGAC+AGG | 0.438232 | 5.2:+68678180 | MS.gene29524:CDS |
| TTAGAAACCCTGACTGAAAG+TGG | 0.461666 | 5.2:-68678071 | None:intergenic |
| GATCAACAACTATCTTAAAT+GGG | 0.468373 | 5.2:-68677893 | None:intergenic |
| TAAGCATCAAAAGGATGATA+AGG | 0.471842 | 5.2:+68678013 | MS.gene29524:CDS |
| GAGAAAAGTCTCATCGGAGT+TGG | 0.476774 | 5.2:-68677566 | None:intergenic |
| ATCGAAAATCACTTGTGCTC+AGG | 0.490320 | 5.2:+68677600 | MS.gene29524:CDS |
| AGAAAAGTCTCATCGGAGTT+GGG | 0.497268 | 5.2:-68677565 | None:intergenic |
| GTCAGGGTTTCTAAGAAGGA+TGG | 0.501378 | 5.2:+68678080 | MS.gene29524:CDS |
| TGAACCCTCGTAGCGGTTGA+CGG | 0.507166 | 5.2:-68677497 | None:intergenic |
| AGGTGAAGAAATTCAGGTTG+AGG | 0.508552 | 5.2:+68677953 | MS.gene29524:CDS |
| CAAGTCAATCACTTTCGTTA+GGG | 0.508696 | 5.2:+68677408 | MS.gene29524:CDS |
| TGAACATGATAAGCATCAAA+AGG | 0.514345 | 5.2:+68678004 | MS.gene29524:CDS |
| ATGCTTATCATGTTCATCAC+CGG | 0.515294 | 5.2:-68677997 | None:intergenic |
| GAAAGCACGGCGCGATGACC+CGG | 0.519271 | 5.2:-68677469 | None:intergenic |
| ACACTTAAAAGAACTTATCA+AGG | 0.539500 | 5.2:+68677933 | MS.gene29524:CDS |
| AAGCATTCCACTTTCAGTCA+GGG | 0.546965 | 5.2:+68678064 | MS.gene29524:CDS |
| AAGTGATTGACTTGGTGCGA+AGG | 0.575471 | 5.2:-68677399 | None:intergenic |
| TGCTGCCGTCAACCGCTACG+AGG | 0.576536 | 5.2:+68677492 | MS.gene29524:CDS |
| TCAGGGTTTCTAAGAAGGAT+GGG | 0.578315 | 5.2:+68678081 | MS.gene29524:CDS |
| TGTTGCTTACCCTGATGAGA+TGG | 0.579238 | 5.2:+68678124 | MS.gene29524:CDS |
| GTTTACAGCGCGGAACTCAT+CGG | 0.581714 | 5.2:-68677626 | None:intergenic |
| GTTGACGGCAGCAGAAAGCA+CGG | 0.587486 | 5.2:-68677482 | None:intergenic |
| GCTGCCGTCAACCGCTACGA+GGG | 0.594490 | 5.2:+68677493 | MS.gene29524:CDS |
| CCTAACGAAAGTGATTGACT+TGG | 0.601517 | 5.2:-68677407 | None:intergenic |
| TAAACGGAGAAAAGTCTCAT+CGG | 0.611707 | 5.2:-68677572 | None:intergenic |
| AACACAATTGAACTCCAAAG+AGG | 0.630953 | 5.2:-68678105 | None:intergenic |
| GATTCATATTAAACAAGCTG+CGG | 0.638671 | 5.2:-68678151 | None:intergenic |
| TCAGAAGAAAAGTGAAACGT+AGG | 0.644962 | 5.2:-68677526 | None:intergenic |
| TGCGGATAACCATCTCATCA+GGG | 0.665501 | 5.2:-68678133 | None:intergenic |
| AAAGTAGAGAATACTTGGTG+TGG | 0.686000 | 5.2:+68678651 | MS.gene29524:CDS |
| GAACAAATGAACCCTCGTAG+CGG | 0.717050 | 5.2:-68677504 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATGTTGAAAATTTGATTT+TGG | + | chr5.2:68677838-68677857 | MS.gene29524:intron | 15.0% |
| !! | AATTGAAAGTAATACAAACA+GGG | - | chr5.2:68678476-68678495 | None:intergenic | 20.0% |
| !! | ATCATGAGTCTAATAAATTA+AGG | - | chr5.2:68677660-68677679 | None:intergenic | 20.0% |
| !! | TAATTGAAAGTAATACAAAC+AGG | - | chr5.2:68678477-68678496 | None:intergenic | 20.0% |
| !!! | AATTTGATTTTGGTTGATAT+AGG | + | chr5.2:68677848-68677867 | MS.gene29524:intron | 20.0% |
| !!! | ATTTGATTTTGGTTGATATA+GGG | + | chr5.2:68677849-68677868 | MS.gene29524:intron | 20.0% |
| !!! | TGATTTTCGATTCAATTAAA+CGG | - | chr5.2:68677591-68677610 | None:intergenic | 20.0% |
| ! | ACACTTAAAAGAACTTATCA+AGG | + | chr5.2:68677933-68677952 | MS.gene29524:CDS | 25.0% |
| ! | AGATCAACAACTATCTTAAA+TGG | - | chr5.2:68677897-68677916 | None:intergenic | 25.0% |
| ! | ATGGTATGGAAATTTGAATT+TGG | + | chr5.2:68677783-68677802 | MS.gene29524:intron | 25.0% |
| ! | GATCAACAACTATCTTAAAT+GGG | - | chr5.2:68677896-68677915 | None:intergenic | 25.0% |
| ! | TTTAATCATACCAGCTTATA+AGG | - | chr5.2:68677747-68677766 | None:intergenic | 25.0% |
| !!! | AACAACTTTGTTATTATGAC+AGG | + | chr5.2:68678180-68678199 | MS.gene29524:CDS | 25.0% |
| AAAAAAGACCCATTTCGTAA+TGG | + | chr5.2:68677764-68677783 | MS.gene29524:intron | 30.0% | |
| ATTGATCAGAAAAACTCAAC+CGG | + | chr5.2:68677450-68677469 | MS.gene29524:CDS | 30.0% | |
| ATTTCCATACCATTACGAAA+TGG | - | chr5.2:68677776-68677795 | None:intergenic | 30.0% | |
| CACAAGTACTTAGAAATAAG+AGG | + | chr5.2:68678578-68678597 | MS.gene29524:CDS | 30.0% | |
| CTAATAAATTAAGGAAGACG+AGG | - | chr5.2:68677651-68677670 | None:intergenic | 30.0% | |
| GATTCATATTAAACAAGCTG+CGG | - | chr5.2:68678154-68678173 | None:intergenic | 30.0% | |
| TAAGCATCAAAAGGATGATA+AGG | + | chr5.2:68678013-68678032 | MS.gene29524:CDS | 30.0% | |
| TGAACATGATAAGCATCAAA+AGG | + | chr5.2:68678004-68678023 | MS.gene29524:CDS | 30.0% | |
| TGAACTTATACCTTATAAGC+TGG | + | chr5.2:68677734-68677753 | MS.gene29524:intron | 30.0% | |
| TTATCAAGGTGAAGAAATTC+AGG | + | chr5.2:68677947-68677966 | MS.gene29524:CDS | 30.0% | |
| TTTCCATACCATTACGAAAT+GGG | - | chr5.2:68677775-68677794 | None:intergenic | 30.0% | |
| ! | GTGCATTCAATATTTGAAAC+CGG | + | chr5.2:68678249-68678268 | MS.gene29524:intron | 30.0% |
| ! | TGCATTCAATATTTGAAACC+GGG | + | chr5.2:68678250-68678269 | MS.gene29524:intron | 30.0% |
| !! | CTCTTATTTCTAAGTACTTG+TGG | - | chr5.2:68678580-68678599 | None:intergenic | 30.0% |
| AACACAATTGAACTCCAAAG+AGG | - | chr5.2:68678108-68678127 | None:intergenic | 35.0% | |
| ATGCTTATCATGTTCATCAC+CGG | - | chr5.2:68678000-68678019 | None:intergenic | 35.0% | |
| CAAGTCAATCACTTTCGTTA+GGG | + | chr5.2:68677408-68677427 | MS.gene29524:CDS | 35.0% | |
| CTGGAAAAGTAGAGAATACT+TGG | + | chr5.2:68678646-68678665 | MS.gene29524:CDS | 35.0% | |
| TAAACGGAGAAAAGTCTCAT+CGG | - | chr5.2:68677575-68677594 | None:intergenic | 35.0% | |
| TCAGAAGAAAAGTGAAACGT+AGG | - | chr5.2:68677529-68677548 | None:intergenic | 35.0% | |
| TCCAAATCCCTGGAAAAAAA+TGG | - | chr5.2:68678536-68678555 | None:intergenic | 35.0% | |
| TTGATCAGAAAAACTCAACC+GGG | + | chr5.2:68677451-68677470 | MS.gene29524:CDS | 35.0% | |
| ! | AAAGTAGAGAATACTTGGTG+TGG | + | chr5.2:68678651-68678670 | MS.gene29524:CDS | 35.0% |
| ! | GCATTCAATATTTGAAACCG+GGG | + | chr5.2:68678251-68678270 | MS.gene29524:intron | 35.0% |
| ! | TGAGGTTGCGATTTTTCATT+TGG | + | chr5.2:68677971-68677990 | MS.gene29524:CDS | 35.0% |
| !! | TGCAAGAAATTTGTTGTGCT+TGG | - | chr5.2:68678611-68678630 | None:intergenic | 35.0% |
| !!! | AGATTCTGCCATTTTTTTCC+AGG | + | chr5.2:68678525-68678544 | MS.gene29524:intron | 35.0% |
| !!! | ATTTTGGTTGATATAGGGTG+AGG | + | chr5.2:68677854-68677873 | MS.gene29524:intron | 35.0% |
| !!! | GATTCTGCCATTTTTTTCCA+GGG | + | chr5.2:68678526-68678545 | MS.gene29524:intron | 35.0% |
| !!! | TGCAGTTCAGATTGTTGTTT+TGG | + | chr5.2:68678434-68678453 | MS.gene29524:CDS | 35.0% |
| AAGCATTCCACTTTCAGTCA+GGG | + | chr5.2:68678064-68678083 | MS.gene29524:CDS | 40.0% | |
| AGAAAAGTCTCATCGGAGTT+GGG | - | chr5.2:68677568-68677587 | None:intergenic | 40.0% | |
| AGACCCATTTCGTAATGGTA+TGG | + | chr5.2:68677769-68677788 | MS.gene29524:intron | 40.0% | |
| ATCGAAAATCACTTGTGCTC+AGG | + | chr5.2:68677600-68677619 | MS.gene29524:CDS | 40.0% | |
| CCAAGTCAATCACTTTCGTT+AGG | + | chr5.2:68677407-68677426 | MS.gene29524:CDS | 40.0% | |
| CCAATCTCAATTCCAAATCG+CGG | - | chr5.2:68678360-68678379 | None:intergenic | 40.0% | |
| CCACTATAGCTGCTATGATA+TGG | - | chr5.2:68678289-68678308 | None:intergenic | 40.0% | |
| CCATATCATAGCAGCTATAG+TGG | + | chr5.2:68678286-68678305 | MS.gene29524:intron | 40.0% | |
| CCTAACGAAAGTGATTGACT+TGG | - | chr5.2:68677410-68677429 | None:intergenic | 40.0% | |
| TTAGAAACCCTGACTGAAAG+TGG | - | chr5.2:68678074-68678093 | None:intergenic | 40.0% | |
| ! | AACTACTAGTTTCCGCGATT+TGG | + | chr5.2:68678345-68678364 | MS.gene29524:intron | 40.0% |
| ! | CGCGATTTGGAATTGAGATT+GGG | + | chr5.2:68678358-68678377 | MS.gene29524:intron | 40.0% |
| ! | GATTTGGAATTGAGATTGGG+TGG | + | chr5.2:68678361-68678380 | MS.gene29524:intron | 40.0% |
| ! | GCGATTTTTCATTTGGTTGC+CGG | + | chr5.2:68677978-68677997 | MS.gene29524:CDS | 40.0% |
| ! | GCTATAGCACTTTTGCATAG+CGG | + | chr5.2:68678312-68678331 | MS.gene29524:intron | 40.0% |
| ! | TCAGGGTTTCTAAGAAGGAT+GGG | + | chr5.2:68678081-68678100 | MS.gene29524:CDS | 40.0% |
| ! | TGCAAGAGTACATGATCAAC+TGG | + | chr5.2:68678627-68678646 | MS.gene29524:CDS | 40.0% |
| ! | TTCAGTCAGGGTTTCTAAGA+AGG | + | chr5.2:68678076-68678095 | MS.gene29524:CDS | 40.0% |
| ! | TTTTTCCAGGGATTTGGATG+AGG | + | chr5.2:68678538-68678557 | MS.gene29524:intron | 40.0% |
| !! | AGGTGAAGAAATTCAGGTTG+AGG | + | chr5.2:68677953-68677972 | MS.gene29524:CDS | 40.0% |
| !!! | GCCATTTTTTTCCAGGGATT+TGG | + | chr5.2:68678532-68678551 | MS.gene29524:intron | 40.0% |
| CAAGCATTCCACTTTCAGTC+AGG | + | chr5.2:68678063-68678082 | MS.gene29524:CDS | 45.0% | |
| GAACAAATGAACCCTCGTAG+CGG | - | chr5.2:68677507-68677526 | None:intergenic | 45.0% | |
| GAGAAAAGTCTCATCGGAGT+TGG | - | chr5.2:68677569-68677588 | None:intergenic | 45.0% | |
| TAAGAAGGATGGGACCTCTT+TGG | + | chr5.2:68678091-68678110 | MS.gene29524:CDS | 45.0% | |
| TATGGTCACTGATGTAACCC+CGG | - | chr5.2:68678271-68678290 | None:intergenic | 45.0% | |
| TCTCTACAACCAAGCAGTAC+AGG | - | chr5.2:68678396-68678415 | None:intergenic | 45.0% | |
| TGCGGATAACCATCTCATCA+GGG | - | chr5.2:68678136-68678155 | None:intergenic | 45.0% | |
| TGTTGCTAACCTGTACTGCT+TGG | + | chr5.2:68678384-68678403 | MS.gene29524:intron | 45.0% | |
| ! | CCGCGATTTGGAATTGAGAT+TGG | + | chr5.2:68678357-68678376 | MS.gene29524:intron | 45.0% |
| ! | GTCAGGGTTTCTAAGAAGGA+TGG | + | chr5.2:68678080-68678099 | MS.gene29524:CDS | 45.0% |
| ! | TGTTGCTTACCCTGATGAGA+TGG | + | chr5.2:68678124-68678143 | MS.gene29524:CDS | 45.0% |
| !! | AAGTGATTGACTTGGTGCGA+AGG | - | chr5.2:68677402-68677421 | None:intergenic | 45.0% |
| CTGCGGATAACCATCTCATC+AGG | - | chr5.2:68678137-68678156 | None:intergenic | 50.0% | |
| GTTTACAGCGCGGAACTCAT+CGG | - | chr5.2:68677629-68677648 | None:intergenic | 50.0% | |
| CAACGCCTCATCCAAATCCC+TGG | - | chr5.2:68678546-68678565 | None:intergenic | 55.0% | |
| GGAAGACGAGGTTTACAGCG+CGG | - | chr5.2:68677639-68677658 | None:intergenic | 55.0% | |
| GTTGACGGCAGCAGAAAGCA+CGG | - | chr5.2:68677485-68677504 | None:intergenic | 55.0% | |
| TGAACCCTCGTAGCGGTTGA+CGG | - | chr5.2:68677500-68677519 | None:intergenic | 55.0% | |
| GCTGCCGTCAACCGCTACGA+GGG | + | chr5.2:68677493-68677512 | MS.gene29524:CDS | 65.0% | |
| TGCTGCCGTCAACCGCTACG+AGG | + | chr5.2:68677492-68677511 | MS.gene29524:CDS | 65.0% | |
| ! | GAAAGCACGGCGCGATGACC+CGG | - | chr5.2:68677472-68677491 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 68677382 | 68678706 | 68677382 | ID=MS.gene29524 |
| chr5.2 | mRNA | 68677382 | 68678706 | 68677382 | ID=MS.gene29524.t1;Parent=MS.gene29524 |
| chr5.2 | exon | 68677382 | 68677642 | 68677382 | ID=MS.gene29524.t1.exon1;Parent=MS.gene29524.t1 |
| chr5.2 | CDS | 68677382 | 68677642 | 68677382 | ID=cds.MS.gene29524.t1;Parent=MS.gene29524.t1 |
| chr5.2 | exon | 68677870 | 68678213 | 68677870 | ID=MS.gene29524.t1.exon2;Parent=MS.gene29524.t1 |
| chr5.2 | CDS | 68677870 | 68678213 | 68677870 | ID=cds.MS.gene29524.t1;Parent=MS.gene29524.t1 |
| chr5.2 | exon | 68678413 | 68678466 | 68678413 | ID=MS.gene29524.t1.exon3;Parent=MS.gene29524.t1 |
| chr5.2 | CDS | 68678413 | 68678466 | 68678413 | ID=cds.MS.gene29524.t1;Parent=MS.gene29524.t1 |
| chr5.2 | exon | 68678547 | 68678706 | 68678547 | ID=MS.gene29524.t1.exon4;Parent=MS.gene29524.t1 |
| chr5.2 | CDS | 68678547 | 68678706 | 68678547 | ID=cds.MS.gene29524.t1;Parent=MS.gene29524.t1 |
| Gene Sequence |
| Protein sequence |
