AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31222

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31222.t1 MTR_2g095750 97.266 512 13 1 1 512 1 511 0.0 1043
MS.gene31222.t1 MTR_2g095730 77.519 516 98 5 1 509 1 505 0.0 824
MS.gene31222.t1 MTR_2g095710 73.663 505 107 6 1 501 1 483 0.0 744
MS.gene31222.t1 MTR_4g057595 68.153 471 141 5 38 503 32 498 0.0 673
MS.gene31222.t1 MTR_1g094155 66.875 480 148 4 39 509 20 497 0.0 671
MS.gene31222.t1 MTR_4g057585 68.696 460 135 5 46 500 39 494 0.0 669
MS.gene31222.t1 MTR_4g057600 66.231 459 148 4 46 500 38 493 0.0 644
MS.gene31222.t1 MTR_1g050545 63.527 499 163 7 14 507 5 489 0.0 641
MS.gene31222.t1 MTR_4g057570 60.480 458 167 8 51 500 40 491 0.0 552
MS.gene31222.t1 MTR_5g097180 32.847 411 256 8 97 499 37 435 1.22e-66 222
MS.gene31222.t1 MTR_4g084710 32.117 411 259 7 97 499 39 437 2.55e-63 213
MS.gene31222.t1 MTR_3g079560 29.812 426 246 14 101 501 92 489 3.26e-52 185
MS.gene31222.t1 MTR_3g434850 30.068 439 244 20 101 503 44 455 3.43e-51 181
MS.gene31222.t1 MTR_3g079590 28.571 448 261 13 79 500 74 488 6.79e-51 181
MS.gene31222.t1 MTR_1g020090 29.929 421 251 11 98 500 101 495 2.97e-50 179
MS.gene31222.t1 MTR_1g021010 29.904 418 252 11 101 500 90 484 4.83e-50 179
MS.gene31222.t1 MTR_8g011350 28.051 467 254 13 101 501 45 495 2.75e-49 177
MS.gene31222.t1 MTR_3g079570 28.859 447 260 14 79 500 75 488 5.02e-49 176
MS.gene31222.t1 MTR_7g051430 27.349 479 263 14 86 498 33 492 7.79e-49 176
MS.gene31222.t1 MTR_3g079600 29.147 422 255 12 101 500 90 489 9.59e-48 172
MS.gene31222.t1 MTR_3g079620 29.515 454 263 14 79 503 74 499 3.88e-47 171
MS.gene31222.t1 MTR_3g034290 28.947 418 264 15 98 498 85 486 9.15e-47 170
MS.gene31222.t1 MTR_3g498755 27.983 461 273 18 72 499 8 442 1.02e-46 169
MS.gene31222.t1 MTR_1g021020 28.947 418 255 11 101 500 92 485 1.39e-46 169
MS.gene31222.t1 MTR_2g081620 28.605 430 260 12 101 503 55 464 1.61e-46 169
MS.gene31222.t1 MTR_2g063000 29.157 439 248 15 101 503 53 464 1.62e-46 169
MS.gene31222.t1 MTR_2g018590 27.609 460 278 12 77 501 30 469 1.69e-46 169
MS.gene31222.t1 MTR_7g021130 29.466 431 248 18 103 499 46 454 2.91e-46 168
MS.gene31222.t1 MTR_1g040370 30.147 408 233 15 103 499 46 412 3.16e-46 167
MS.gene31222.t1 MTR_2g062990 28.972 428 261 13 101 503 54 463 5.52e-46 167
MS.gene31222.t1 MTR_2g449850 29.091 440 249 18 96 499 47 459 6.08e-46 167
MS.gene31222.t1 MTR_7g051430 34.944 269 153 5 86 349 33 284 8.52e-46 166
MS.gene31222.t1 MTR_8g099930 28.111 434 256 18 101 499 47 459 6.03e-45 164
MS.gene31222.t1 MTR_4g122960 26.786 448 263 13 99 503 51 476 1.16e-44 164
MS.gene31222.t1 MTR_0003s0560 28.009 432 258 14 93 498 79 483 1.38e-44 164
MS.gene31222.t1 MTR_2g062980 28.211 436 254 13 101 503 54 463 2.00e-44 163
MS.gene31222.t1 MTR_4g122950 26.682 446 268 13 99 503 51 478 5.17e-44 162
MS.gene31222.t1 MTR_3g079630 28.097 452 266 15 79 500 74 496 5.63e-44 162
MS.gene31222.t1 MTR_5g072570 28.222 450 290 14 78 501 18 460 1.55e-43 160
MS.gene31222.t1 MTR_4g089155 26.411 443 271 12 93 499 59 482 1.80e-43 160
MS.gene31222.t1 MTR_2g018600 26.207 435 270 12 103 501 50 469 2.91e-43 160
MS.gene31222.t1 MTR_7g080180 36.782 261 141 8 101 349 42 290 3.50e-43 159
MS.gene31222.t1 MTR_4g075150 28.806 427 260 14 101 499 48 458 6.58e-43 159
MS.gene31222.t1 MTR_7g080180 37.165 261 140 9 101 349 42 290 6.68e-43 159
MS.gene31222.t1 MTR_2g018630 28.132 423 266 12 103 499 53 463 3.54e-42 157
MS.gene31222.t1 MTR_4g075150 28.074 431 260 13 101 499 48 460 3.97e-42 157
MS.gene31222.t1 MTR_8g011350 27.273 440 238 13 101 474 45 468 6.78e-42 156
MS.gene31222.t1 MTR_4g021510 29.103 457 267 18 72 495 31 463 1.25e-41 155
MS.gene31222.t1 MTR_1g095560 26.887 424 268 14 101 499 47 453 1.80e-41 155
MS.gene31222.t1 MTR_3g090130 27.563 439 271 14 93 501 34 455 1.92e-41 155
MS.gene31222.t1 MTR_3g045770 27.938 451 277 12 86 501 17 454 3.33e-41 154
MS.gene31222.t1 MTR_2g012860 25.113 442 278 11 93 499 47 470 6.09e-41 154
MS.gene31222.t1 MTR_4g021530 28.502 414 247 14 115 496 69 465 6.35e-41 153
MS.gene31222.t1 MTR_7g013790 27.294 436 253 14 103 499 51 461 6.13e-40 150
MS.gene31222.t1 MTR_2g012850 26.037 434 266 12 101 499 49 462 7.34e-40 150
MS.gene31222.t1 MTR_3g090150 27.739 429 285 12 93 501 39 462 8.82e-40 150
MS.gene31222.t1 MTR_4g021490 27.511 458 275 17 72 496 32 465 1.44e-39 150
MS.gene31222.t1 MTR_4g021535 27.230 426 237 13 115 496 65 461 2.11e-39 149
MS.gene31222.t1 MTR_4g021520 27.423 423 265 14 103 496 56 465 3.63e-39 149
MS.gene31222.t1 MTR_1g095540 25.822 426 270 11 101 499 47 453 2.60e-38 146
MS.gene31222.t1 MTR_1g095560 26.398 447 273 16 101 499 47 485 5.94e-38 145
MS.gene31222.t1 MTR_3g034290 29.552 335 209 13 98 416 85 408 6.96e-38 144
MS.gene31222.t1 MTR_4g021500 26.840 462 290 16 72 504 32 474 3.78e-37 146
MS.gene31222.t1 MTR_4g021500 26.277 411 260 12 115 496 504 900 8.15e-37 145
MS.gene31222.t1 MTR_4g021500 26.840 462 290 16 72 504 32 474 3.88e-37 146
MS.gene31222.t1 MTR_4g021500 26.515 396 260 13 115 496 504 882 1.73e-35 141
MS.gene31222.t1 MTR_3g100420 25.951 447 283 11 86 496 57 491 9.01e-37 142
MS.gene31222.t1 MTR_4g021520 27.720 386 238 13 103 460 56 428 1.41e-33 132
MS.gene31222.t1 MTR_0003s0560 30.859 256 158 7 93 345 79 318 1.97e-33 130
MS.gene31222.t1 MTR_2g063000 25.917 436 235 14 101 503 53 433 2.32e-33 132
MS.gene31222.t1 MTR_4g056520 26.824 425 256 12 114 497 44 454 7.86e-33 130
MS.gene31222.t1 MTR_2g009200 24.401 459 270 15 74 497 19 435 7.93e-30 122
MS.gene31222.t1 MTR_2g084530 26.339 448 280 13 84 492 11 447 1.01e-25 110
MS.gene31222.t1 MTR_2g018630 25.419 358 234 10 163 499 13 358 3.17e-24 104
MS.gene31222.t1 MTR_1g102130 24.545 440 230 20 103 499 107 487 7.14e-23 102
MS.gene31222.t1 MTR_4g021535 23.474 426 206 16 115 496 65 414 3.41e-20 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31222.t1 AT3G45010 66.068 501 160 5 12 505 8 505 0.0 691
MS.gene31222.t1 AT3G10410 62.795 508 166 6 5 502 4 498 0.0 676
MS.gene31222.t1 AT5G22980 62.450 498 176 5 13 503 5 498 0.0 644
MS.gene31222.t1 AT5G22960 62.874 167 42 2 95 261 28 174 1.63e-67 216
MS.gene31222.t1 AT1G15000 30.488 410 262 9 97 499 31 424 6.85e-54 188
MS.gene31222.t1 AT1G73300 29.291 437 253 14 86 499 36 439 1.87e-52 184
MS.gene31222.t1 AT1G73300 29.291 437 253 14 86 499 36 439 1.87e-52 184
MS.gene31222.t1 AT3G25420 28.541 466 253 13 101 501 44 494 2.34e-52 185
MS.gene31222.t1 AT3G12203 27.460 437 267 15 89 499 2 414 2.13e-51 181
MS.gene31222.t1 AT3G12203 27.460 437 267 15 89 499 23 435 5.69e-51 180
MS.gene31222.t1 AT2G22920 29.425 435 278 14 73 498 18 432 1.01e-49 177
MS.gene31222.t1 AT3G12203 27.930 401 248 14 116 499 1 377 2.41e-49 174
MS.gene31222.t1 AT1G73280 28.474 439 256 15 85 499 35 439 2.42e-49 176
MS.gene31222.t1 AT2G22970 28.281 442 274 16 73 499 18 431 2.81e-49 175
MS.gene31222.t1 AT5G36180 28.539 438 257 14 85 499 35 439 2.92e-49 176
MS.gene31222.t1 AT3G17180 30.023 433 262 15 98 499 50 472 6.03e-49 176
MS.gene31222.t1 AT1G73290 27.356 435 262 14 86 499 35 436 6.36e-49 174
MS.gene31222.t1 AT4G12910 28.216 482 258 15 86 501 38 497 2.32e-48 174
MS.gene31222.t1 AT2G22980 28.315 445 267 18 73 499 18 428 5.04e-48 172
MS.gene31222.t1 AT5G23210 29.933 451 249 15 101 503 65 496 1.29e-47 172
MS.gene31222.t1 AT3G52000 28.125 416 261 11 101 497 78 474 1.54e-47 172
MS.gene31222.t1 AT1G73270 25.977 435 276 13 85 501 35 441 1.61e-47 171
MS.gene31222.t1 AT1G73270 25.977 435 276 13 85 501 35 441 2.27e-47 171
MS.gene31222.t1 AT2G23010 27.578 446 276 15 73 499 18 435 3.51e-47 170
MS.gene31222.t1 AT2G23010 27.578 446 276 15 73 499 18 435 9.46e-47 169
MS.gene31222.t1 AT1G73310 27.578 417 256 11 103 499 49 439 1.00e-46 169
MS.gene31222.t1 AT4G15100 28.037 428 270 13 101 498 58 477 1.05e-46 170
MS.gene31222.t1 AT3G10450 27.546 432 269 16 85 499 31 435 1.76e-46 168
MS.gene31222.t1 AT3G10450 27.184 412 260 14 105 499 4 392 6.10e-46 165
MS.gene31222.t1 AT1G28110 28.438 429 257 17 101 498 45 454 9.41e-46 166
MS.gene31222.t1 AT1G28110 28.438 429 257 17 101 498 45 454 9.41e-46 166
MS.gene31222.t1 AT4G30810 28.046 435 264 13 99 504 50 464 1.63e-45 166
MS.gene31222.t1 AT4G12910 36.059 269 150 6 86 349 38 289 1.78e-45 166
MS.gene31222.t1 AT2G33530 28.704 432 256 17 101 499 47 459 1.81e-45 166
MS.gene31222.t1 AT3G63470 29.356 419 243 15 101 492 95 487 2.20e-45 166
MS.gene31222.t1 AT1G33540 27.689 437 257 15 103 499 45 462 2.46e-45 166
MS.gene31222.t1 AT1G73290 28.877 374 219 11 86 439 35 381 2.62e-45 164
MS.gene31222.t1 AT2G22920 29.455 404 246 14 73 462 18 396 2.77e-45 164
MS.gene31222.t1 AT3G52020 28.205 429 248 13 101 497 93 493 6.63e-45 165
MS.gene31222.t1 AT1G33540 27.586 435 256 15 105 499 4 419 8.91e-45 163
MS.gene31222.t1 AT2G22970 28.571 420 258 15 73 477 18 410 9.87e-45 163
MS.gene31222.t1 AT2G22970 28.401 419 258 15 73 476 18 409 1.18e-44 162
MS.gene31222.t1 AT2G22980 28.182 440 264 18 73 494 18 423 1.83e-44 162
MS.gene31222.t1 AT5G08260 30.345 435 239 17 101 492 53 466 2.99e-44 163
MS.gene31222.t1 AT2G22980 28.199 422 252 17 73 476 18 406 3.15e-44 161
MS.gene31222.t1 AT2G35770 30.073 409 247 13 103 489 54 445 3.70e-44 162
MS.gene31222.t1 AT2G22990 26.139 417 264 12 103 499 39 431 6.66e-44 161
MS.gene31222.t1 AT2G23000 27.149 442 265 13 90 499 19 435 1.21e-43 160
MS.gene31222.t1 AT2G23000 27.149 442 265 13 90 499 19 435 1.21e-43 160
MS.gene31222.t1 AT3G12240 27.804 428 243 17 102 499 43 434 2.11e-43 159
MS.gene31222.t1 AT3G52010 27.934 426 268 12 92 497 73 479 7.00e-43 159
MS.gene31222.t1 AT1G61130 28.037 428 262 14 101 498 45 456 9.66e-43 158
MS.gene31222.t1 AT1G73310 27.632 380 231 9 103 463 49 403 1.70e-42 157
MS.gene31222.t1 AT1G11080 27.972 429 271 13 101 498 64 485 2.22e-42 158
MS.gene31222.t1 AT3G12230 27.295 414 263 12 102 499 42 433 2.33e-42 157
MS.gene31222.t1 AT1G11080 27.972 429 271 13 101 498 64 485 2.69e-42 158
MS.gene31222.t1 AT3G10450 27.384 409 254 15 85 476 31 413 4.93e-42 155
MS.gene31222.t1 AT1G73290 32.353 272 159 7 86 343 35 295 6.49e-42 152
MS.gene31222.t1 AT5G23210 30.790 367 196 12 101 421 65 419 7.18e-42 156
MS.gene31222.t1 AT2G24000 27.506 429 261 16 103 498 46 457 1.16e-41 155
MS.gene31222.t1 AT3G12220 25.701 428 284 14 86 501 30 435 1.69e-41 154
MS.gene31222.t1 AT1G43780 28.111 434 256 17 103 499 53 467 1.88e-41 155
MS.gene31222.t1 AT3G10450 26.992 389 245 13 105 476 4 370 2.26e-41 152
MS.gene31222.t1 AT2G24010 28.235 425 255 15 103 499 46 448 2.33e-41 154
MS.gene31222.t1 AT5G42230 28.372 430 267 16 101 501 42 459 5.63e-41 154
MS.gene31222.t1 AT5G42230 28.372 430 267 16 101 501 42 459 5.63e-41 154
MS.gene31222.t1 AT3G12220 25.183 409 276 12 105 501 15 405 9.16e-41 152
MS.gene31222.t1 AT2G12480 28.117 409 261 12 103 501 47 432 1.28e-40 152
MS.gene31222.t1 AT2G12480 27.857 420 272 13 103 501 47 456 1.67e-40 152
MS.gene31222.t1 AT3G07990 27.553 421 275 13 98 499 45 454 1.82e-40 152
MS.gene31222.t1 AT4G30610 25.293 427 282 13 99 499 44 459 3.26e-40 151
MS.gene31222.t1 AT2G22990 26.131 398 249 11 103 478 39 413 4.08e-40 150
MS.gene31222.t1 AT2G22990 26.521 411 255 13 86 476 26 409 4.55e-40 151
MS.gene31222.t1 AT3G17180 30.607 379 228 13 98 446 50 423 7.87e-40 150
MS.gene31222.t1 AT2G12480 27.160 405 268 11 103 501 47 430 9.60e-40 149
MS.gene31222.t1 AT2G22920 37.931 232 132 7 73 299 18 242 3.60e-39 145
MS.gene31222.t1 AT2G12480 26.847 406 269 11 103 501 47 431 4.39e-39 148
MS.gene31222.t1 AT1G73280 32.288 319 174 10 85 383 35 331 8.19e-39 145
MS.gene31222.t1 AT5G09640 25.160 469 285 17 73 499 19 463 9.04e-39 147
MS.gene31222.t1 AT5G42240 25.935 428 280 14 101 501 46 463 2.12e-38 146
MS.gene31222.t1 AT2G22970 39.216 204 112 6 73 271 18 214 7.26e-38 141
MS.gene31222.t1 AT5G23210 29.298 413 226 14 138 503 8 401 1.92e-37 142
MS.gene31222.t1 AT2G22990 24.700 417 261 13 103 499 39 422 3.07e-37 142
MS.gene31222.t1 AT3G02110 26.316 437 261 12 103 499 53 468 3.36e-37 143
MS.gene31222.t1 AT2G35780 25.701 428 270 11 99 499 42 448 4.82e-37 142
MS.gene31222.t1 AT2G24010 27.273 440 255 17 103 499 46 463 1.10e-36 142
MS.gene31222.t1 AT2G22980 31.329 316 181 11 73 375 18 310 1.10e-36 139
MS.gene31222.t1 AT5G23210 29.126 412 226 14 139 503 8 400 1.13e-36 140
MS.gene31222.t1 AT2G05850 26.364 440 282 16 78 497 62 479 8.90e-36 139
MS.gene31222.t1 AT2G24010 26.797 459 258 19 103 499 17 459 2.20e-33 132
MS.gene31222.t1 AT3G12220 33.010 206 131 4 102 301 42 246 1.26e-32 129
MS.gene31222.t1 AT1G73280 26.124 356 214 11 163 499 12 337 5.78e-32 125
MS.gene31222.t1 AT3G56540 42.553 141 78 3 101 239 93 232 1.38e-31 123
MS.gene31222.t1 AT5G36180 26.197 355 215 10 163 499 12 337 1.63e-31 124
MS.gene31222.t1 AT5G36180 26.197 355 215 10 163 499 12 337 1.63e-31 124
MS.gene31222.t1 AT5G23210 29.878 328 173 11 139 421 8 323 4.69e-31 124
MS.gene31222.t1 AT2G27920 26.366 421 255 15 114 492 45 452 2.94e-28 117
MS.gene31222.t1 AT2G24010 34.101 217 121 7 103 299 17 231 5.58e-28 115
MS.gene31222.t1 AT1G73280 25.146 342 207 11 177 499 1 312 7.27e-28 114
MS.gene31222.t1 AT5G36180 25.904 332 200 9 163 476 12 315 1.67e-27 113
MS.gene31222.t1 AT2G22990 23.529 340 221 10 175 499 2 317 2.98e-27 112
MS.gene31222.t1 AT2G22990 23.529 340 221 10 175 499 2 317 7.24e-27 111
MS.gene31222.t1 AT5G36180 26.667 315 186 8 163 460 12 298 2.38e-26 110
MS.gene31222.t1 AT1G73310 25.079 315 197 7 163 463 12 301 2.78e-25 106
MS.gene31222.t1 AT2G22980 24.522 314 199 10 175 476 2 289 1.49e-24 103
MS.gene31222.t1 AT2G27920 25.521 384 237 13 145 492 10 380 6.84e-22 98.2
MS.gene31222.t1 AT2G27920 24.709 344 218 11 184 492 48 385 9.34e-17 82.8

Find 116 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTCTCTGGTGTGTTTA+TGG 0.125077 2.4:-7032736 None:intergenic
ACAACATGGCATCATCTTTA+AGG 0.142828 2.4:+7032541 None:intergenic
TCTTCACTATCAACCAAATA+TGG 0.169918 2.4:-7035760 None:intergenic
GTCTCCTACAACCCCATATT+TGG 0.211172 2.4:+7035747 MS.gene31222:CDS
TATGCCTTGTACTGAATTTC+CGG 0.228537 2.4:-7034759 None:intergenic
TCCCTTGTTTGGAATGAATA+TGG 0.250663 2.4:+7033588 MS.gene31222:CDS
CACAAGATTCTATAGCCTTT+TGG 0.276751 2.4:-7034857 None:intergenic
AGCTTTAGGTGTTGGGGACT+TGG 0.302455 2.4:+7035267 MS.gene31222:CDS
TTATCCTTGGAGAATTTAAA+AGG 0.303425 2.4:-7033558 None:intergenic
AGGATGACCCTGTTGTTATA+TGG 0.306709 2.4:+7033481 MS.gene31222:CDS
ATAAGAACTTGTCCCTTGTT+TGG 0.312379 2.4:+7033577 MS.gene31222:CDS
ATATCATACATTAATGTTGC+CGG 0.346161 2.4:-7035074 None:intergenic
AGACAATGGACTGATTAATA+AGG 0.347573 2.4:+7034797 MS.gene31222:CDS
CCTGTTGTTATATGGTTAAC+TGG 0.347839 2.4:+7033489 MS.gene31222:CDS
TCTTGAACATGAAGACAGTT+AGG 0.353758 2.4:+7035242 MS.gene31222:CDS
AACATCTGAAGTGCAGCTTT+TGG 0.363126 2.4:-7036120 None:intergenic
TGAAATATTCGATCAGATAA+TGG 0.371508 2.4:+7035045 MS.gene31222:CDS
CACTTCAGATGTTAAAAGAT+TGG 0.373515 2.4:+7036130 MS.gene31222:CDS
CTTCACTATCAACCAAATAT+GGG 0.374075 2.4:-7035759 None:intergenic
TTAGGGAAGCTTTAGGTGTT+GGG 0.376792 2.4:+7035260 MS.gene31222:CDS
CGATCTATATGACTTCTTGC+AGG 0.377801 2.4:+7034262 MS.gene31222:CDS
CCATTGTCTAATGCAAAATC+TGG 0.382981 2.4:-7034783 None:intergenic
AAGACAGTTAGGGAAGCTTT+AGG 0.386288 2.4:+7035253 MS.gene31222:CDS
CCAGTTAACCATATAACAAC+AGG 0.402521 2.4:-7033489 None:intergenic
AGTATACAGTGCCATGTTGC+AGG 0.410064 2.4:+7035312 MS.gene31222:CDS
TTACAAAAGACAGACACATT+TGG 0.417560 2.4:-7032569 None:intergenic
GAGGAGAATGTCATTGTTTG+TGG 0.420601 2.4:-7032637 None:intergenic
AAGGGACAAGTTCTTATCCT+TGG 0.432855 2.4:-7033571 None:intergenic
CTCGTTTGGCAGGGAATTCG+AGG 0.435718 2.4:+7035680 MS.gene31222:intron
GTAGACCAACCCACTGGAAC+AGG 0.438401 2.4:+7034182 MS.gene31222:CDS
GTTAGGGAAGCTTTAGGTGT+TGG 0.441008 2.4:+7035259 MS.gene31222:CDS
GGCTGGTCACATGGTTCCTA+TGG 0.441603 2.4:+7036092 MS.gene31222:CDS
TTTGATTTGTTAGGCACTAA+AGG 0.444714 2.4:+7034986 MS.gene31222:intron
GGTGTGTTTATGGAATCTTT+TGG 0.445106 2.4:-7032726 None:intergenic
CTTTGAATCTCGAAACACTA+AGG 0.445707 2.4:+7033461 MS.gene31222:CDS
TCAACCAAATATGGGGTTGT+AGG 0.451088 2.4:-7035751 None:intergenic
AAGAAGATCTCATATGCAAT+TGG 0.456279 2.4:+7035410 MS.gene31222:CDS
GAAAAGAAGTTCACTTTCCC+TGG 0.457022 2.4:+7032786 MS.gene31222:CDS
ATCAAGGTGCTTGTGTATGC+TGG 0.464364 2.4:+7035385 MS.gene31222:CDS
CAAGCAGAAAATCTCATAAG+AGG 0.468099 2.4:+7032690 MS.gene31222:CDS
GGTGCTTCTGTTGAAGAACT+TGG 0.468257 2.4:+7032822 MS.gene31222:CDS
TCATTGTTTGTGGGATAAGC+TGG 0.472247 2.4:-7032627 None:intergenic
ATTCCTGCTCTTCTTGAGGA+TGG 0.475493 2.4:+7035361 MS.gene31222:CDS
TTGGTTGATAGTGAAGAAGC+AGG 0.484292 2.4:+7035766 MS.gene31222:CDS
CGGACCTCTTGCTTTCCTCA+AGG 0.484984 2.4:+7035804 MS.gene31222:CDS
GTTCAAGAATGTTTCCGCAT+TGG 0.488329 2.4:-7035227 None:intergenic
ACAGTGCCATGTTGCAGGAT+TGG 0.491110 2.4:+7035317 MS.gene31222:CDS
TACCTTGCAGCTTCGGAATG+AGG 0.492745 2.4:-7032870 None:intergenic
CTTGAACATGAAGACAGTTA+GGG 0.500091 2.4:+7035243 MS.gene31222:CDS
AGGAATTCATATAAATCTAA+AGG 0.501724 2.4:+7034599 MS.gene31222:CDS
ACAAAATGAGGATCATTCTC+TGG 0.506628 2.4:-7032747 None:intergenic
CTTGTACTGAATTTCCGGAT+TGG 0.507308 2.4:-7034754 None:intergenic
CAAGGAAACAAGGCAAAAGA+AGG 0.507437 2.4:+7034579 MS.gene31222:CDS
GGGTATTCCTGCTCTTCTTG+AGG 0.508272 2.4:+7035357 MS.gene31222:CDS
GCAATTTCACTGCTGCATCC+TGG 0.508823 2.4:-7033516 None:intergenic
GGATGCAAGGGAAACTGATA+AGG 0.515489 2.4:+7036151 MS.gene31222:CDS
TGAACCCTAGATGCAAAAGC+TGG 0.524049 2.4:-7034555 None:intergenic
ATATGGTTAACTGGAGGGCC+AGG 0.526048 2.4:+7033498 MS.gene31222:CDS
ATTCCATCCTCAAGAAGAGC+AGG 0.526347 2.4:-7035364 None:intergenic
GTTGTTCCCACGATGCCAAA+AGG 0.528378 2.4:+7034842 MS.gene31222:CDS
ATGTCCAGCGTATGATTCTC+CGG 0.529003 2.4:-7034526 None:intergenic
AAGATGATGCAGTTGAAGAG+AGG 0.530703 2.4:-7032656 None:intergenic
TCTTCTTGAGGATGGAATCA+AGG 0.531157 2.4:+7035369 MS.gene31222:CDS
GAAGAACTTGGTCATCATGC+AGG 0.536316 2.4:+7032834 MS.gene31222:CDS
GTTGATGCCATGACATGGTC+AGG 0.537146 2.4:+7035706 MS.gene31222:CDS
TAGGGAAGCTTTAGGTGTTG+GGG 0.537357 2.4:+7035261 MS.gene31222:CDS
TGAAACCTGTTCCAGTGGGT+TGG 0.537449 2.4:-7034187 None:intergenic
ATGTTAAAAGATTGGATGCA+AGG 0.542286 2.4:+7036138 MS.gene31222:CDS
AATCATTCTTAGTGAACTGA+GGG 0.546470 2.4:-7034494 None:intergenic
CAATCCGGAAATTCAGTACA+AGG 0.549343 2.4:+7034755 MS.gene31222:CDS
GCACCAGAGTCAACAAAACC+AGG 0.549754 2.4:-7032804 None:intergenic
TTGTTTGGAATGAATATGGA+TGG 0.552020 2.4:+7033592 MS.gene31222:CDS
AGGAGAATGTCATTGTTTGT+GGG 0.552520 2.4:-7032636 None:intergenic
TGGTAACGGATTAACCAATC+CGG 0.554595 2.4:+7034740 MS.gene31222:CDS
GACATTAGAAAGCAATGTGT+GGG 0.565208 2.4:+7035184 MS.gene31222:CDS
TTGTTATATGGTTAACTGGA+GGG 0.566502 2.4:+7033493 MS.gene31222:CDS
TTTGCATCTAGGGTTCATCA+AGG 0.567295 2.4:+7034561 MS.gene31222:CDS
GATCTCATATGCAATTGGCT+TGG 0.569300 2.4:+7035415 MS.gene31222:CDS
TGTTAAAAGATTGGATGCAA+GGG 0.569319 2.4:+7036139 MS.gene31222:CDS
ATATTTCCATGAACAAAATG+AGG 0.569648 2.4:-7032759 None:intergenic
TTTGGCAGGGAATTCGAGGT+GGG 0.572491 2.4:+7035684 MS.gene31222:intron
GTTGTTATATGGTTAACTGG+AGG 0.581708 2.4:+7033492 MS.gene31222:CDS
CAAAAGACAGACACATTTGG+AGG 0.583029 2.4:-7032566 None:intergenic
GTTTGGCAGGGAATTCGAGG+TGG 0.583856 2.4:+7035683 MS.gene31222:intron
TTCCTCATTCCGAAGCTGCA+AGG 0.587932 2.4:+7032868 MS.gene31222:CDS
TGGGAACAACTTGTTGATGT+TGG 0.588848 2.4:-7034829 None:intergenic
GGTGAAAGAGGCTGGTCACA+TGG 0.589518 2.4:+7036083 MS.gene31222:intron
AGAATGAATGTGAAATGAAG+AGG 0.598446 2.4:-7032599 None:intergenic
TTCACTATCAACCAAATATG+GGG 0.599058 2.4:-7035758 None:intergenic
ATATTTGTAGACCAACCCAC+TGG 0.600249 2.4:+7034176 MS.gene31222:CDS
ATCCATATTCATTCCAAACA+AGG 0.604369 2.4:-7033590 None:intergenic
TTTCATCCAATCCTGCAACA+TGG 0.613087 2.4:-7035323 None:intergenic
TGACATTAGAAAGCAATGTG+TGG 0.619542 2.4:+7035183 MS.gene31222:CDS
GATTTGTTAGGCACTAAAGG+TGG 0.621116 2.4:+7034989 MS.gene31222:intron
GATCAGATAATGGACATAGC+CGG 0.623123 2.4:+7035055 MS.gene31222:CDS
AAATCATTCTTAGTGAACTG+AGG 0.624376 2.4:-7034495 None:intergenic
CAAAAGGCTATAGAATCTTG+TGG 0.625610 2.4:+7034858 MS.gene31222:CDS
TAGCTGAAACCTGTTCCAGT+GGG 0.633323 2.4:-7034191 None:intergenic
ATAGCTGAAACCTGTTCCAG+TGG 0.637939 2.4:-7034192 None:intergenic
TCCATATTCATTCCAAACAA+GGG 0.638155 2.4:-7033589 None:intergenic
TCGAACGTCGCTATCATCAG+TGG 0.646032 2.4:-7034216 None:intergenic
TAGGGTTCATCAAGGAAACA+AGG 0.646926 2.4:+7034569 MS.gene31222:CDS
AAGGGAAACTGATAAGGACA+AGG 0.660416 2.4:+7036157 MS.gene31222:CDS
CACCAGAGTCAACAAAACCA+GGG 0.660712 2.4:-7032803 None:intergenic
AAACTGATAAGGACAAGGGG+AGG 0.667086 2.4:+7036162 MS.gene31222:CDS
AGGGAAACTGATAAGGACAA+GGG 0.667941 2.4:+7036158 MS.gene31222:CDS
GGTGGGTTGATGCCATGACA+TGG 0.669211 2.4:+7035701 MS.gene31222:CDS
TGAGGAAAGCAAGAGGTCCG+TGG 0.669417 2.4:-7035801 None:intergenic
TTGTTATGACTTCTCCAATG+CGG 0.671699 2.4:+7035213 MS.gene31222:CDS
TGTTTGGAATGAATATGGAT+GGG 0.672561 2.4:+7033593 MS.gene31222:CDS
GAATGAATATGGATGGGACA+TGG 0.683982 2.4:+7033599 MS.gene31222:CDS
ATTACCGGAGAATCATACGC+TGG 0.686475 2.4:+7034522 MS.gene31222:CDS
GGGAAACTGATAAGGACAAG+GGG 0.694048 2.4:+7036159 MS.gene31222:CDS
CAAACCTTGAGGAAAGCAAG+AGG 0.699262 2.4:-7035808 None:intergenic
GACGTTCGACATGATGAAGA+CGG 0.699400 2.4:+7034230 MS.gene31222:CDS
CAGTTAACCATATAACAACA+GGG 0.704037 2.4:-7033488 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAGAATGAAATATT+AGG + chr2.4:7032934-7032953 MS.gene31222:intron 10.0%
!! AGAAAAGAGAATAATTTAAT+AGG - chr2.4:7033627-7033646 None:intergenic 15.0%
!! AGCAAAGAATTTATAAAAAA+TGG + chr2.4:7033071-7033090 MS.gene31222:intron 15.0%
!! TTATAAAAAATGGAATAGTT+TGG + chr2.4:7033081-7033100 MS.gene31222:intron 15.0%
!!! AAAGTGTTTTTCTTAAAATT+CGG - chr2.4:7033288-7033307 None:intergenic 15.0%
!!! AAGAATGATTTTTATATTAC+CGG + chr2.4:7034507-7034526 MS.gene31222:CDS 15.0%
!!! CATTCTATTTTATCTTATTT+TGG - chr2.4:7032927-7032946 None:intergenic 15.0%
!!! CTCTATTGATTTATATTAAT+TGG + chr2.4:7033348-7033367 MS.gene31222:intron 15.0%
!!! TTAGCTATTTTTACAATAAA+AGG - chr2.4:7033893-7033912 None:intergenic 15.0%
!!! TTTGATCTAGTTTAATTTAT+TGG + chr2.4:7033118-7033137 MS.gene31222:intron 15.0%
!! AAGGAAAAATAACAAGTAAA+TGG - chr2.4:7034946-7034965 None:intergenic 20.0%
!! AATTATTCACTTGTATTAAG+TGG + chr2.4:7034356-7034375 MS.gene31222:intron 20.0%
!! AGGAATTCATATAAATCTAA+AGG + chr2.4:7034599-7034618 MS.gene31222:CDS 20.0%
!! CAAAATAGAATACACAAATA+TGG + chr2.4:7035634-7035653 MS.gene31222:intron 20.0%
!! CAGAATAAAAATATAGTTCT+TGG + chr2.4:7033010-7033029 MS.gene31222:intron 20.0%
!! GATTATAAGTTTGACTAATT+TGG + chr2.4:7035593-7035612 MS.gene31222:intron 20.0%
!! GTAAACCTTTATAATAAACA+GGG + chr2.4:7034708-7034727 MS.gene31222:intron 20.0%
!! TCTTTCACCTATTAAAAAAA+CGG - chr2.4:7036072-7036091 None:intergenic 20.0%
!! TGTAAACCTTTATAATAAAC+AGG + chr2.4:7034707-7034726 MS.gene31222:intron 20.0%
!! TGTACACTATATTTATGTAT+AGG + chr2.4:7034643-7034662 MS.gene31222:intron 20.0%
!! TTTCATAAGCTATATTAGTT+TGG - chr2.4:7033853-7033872 None:intergenic 20.0%
!!! AATATTAGGTAAGTTTTTTG+TGG + chr2.4:7032948-7032967 MS.gene31222:intron 20.0%
!!! ATTGCTATGTTTTATGAAAA+TGG + chr2.4:7033534-7033553 MS.gene31222:CDS 20.0%
!!! TAGTTTGTATTTGATTTGTT+AGG + chr2.4:7034977-7034996 MS.gene31222:intron 20.0%
!!! TATTTTGACACAAGAAATTT+TGG - chr2.4:7035621-7035640 None:intergenic 20.0%
!!! TTAACTTCCGTTTTTTTAAT+AGG + chr2.4:7036062-7036081 MS.gene31222:intron 20.0%
!!! TTAAGTTGTTTATCCAAATA+TGG + chr2.4:7033958-7033977 MS.gene31222:intron 20.0%
!!! TTGTCATCAAATTCTTATTT+TGG - chr2.4:7033328-7033347 None:intergenic 20.0%
! ACAAACAAGAAAAAATGCAT+TGG - chr2.4:7034142-7034161 None:intergenic 25.0%
! ACAAACTATTAGTGTCAATA+AGG - chr2.4:7034965-7034984 None:intergenic 25.0%
! ACAACTTATGTCATATTTGT+AGG + chr2.4:7033775-7033794 MS.gene31222:intron 25.0%
! ACATAAGCATTTATATGACA+AGG + chr2.4:7033914-7033933 MS.gene31222:intron 25.0%
! ATATCATACATTAATGTTGC+CGG - chr2.4:7035077-7035096 None:intergenic 25.0%
! ATATTTCCATGAACAAAATG+AGG - chr2.4:7032762-7032781 None:intergenic 25.0%
! CAAAACCCTGTTTATTATAA+AGG - chr2.4:7034716-7034735 None:intergenic 25.0%
! CATAAGCATTTATATGACAA+GGG + chr2.4:7033915-7033934 MS.gene31222:intron 25.0%
! TATATGAGTAAGAACTAAAG+TGG - chr2.4:7033660-7033679 None:intergenic 25.0%
! TGAAATATTCGATCAGATAA+TGG + chr2.4:7035045-7035064 MS.gene31222:CDS 25.0%
! TTATAAATGTGATTCTCGTT+TGG + chr2.4:7035666-7035685 MS.gene31222:intron 25.0%
! TTATCCTTGGAGAATTTAAA+AGG - chr2.4:7033561-7033580 None:intergenic 25.0%
!! AGATTTTCTGCTTGTAATTT+TGG - chr2.4:7032684-7032703 None:intergenic 25.0%
!! CATGACTATCTTTGAATTTT+AGG + chr2.4:7032901-7032920 MS.gene31222:intron 25.0%
!! GATTTTCTGCTTGTAATTTT+GGG - chr2.4:7032683-7032702 None:intergenic 25.0%
!! TCTTAAAATTCGGTCTATTT+TGG - chr2.4:7033278-7033297 None:intergenic 25.0%
!! TTTTGTTCATGGAAATATTG+TGG + chr2.4:7032764-7032783 MS.gene31222:CDS 25.0%
!!! AGACTGTTTGAGAATTTTTT+TGG + chr2.4:7033750-7033769 MS.gene31222:intron 25.0%
!!! ATGCATTTTTTCTTGTTTGT+AGG + chr2.4:7034142-7034161 MS.gene31222:intron 25.0%
!!! GTTTTTTTAATAGGTGAAAG+AGG + chr2.4:7036071-7036090 MS.gene31222:intron 25.0%
!!! TATTTGTAGGTTTTTTTGAG+AGG + chr2.4:7033788-7033807 MS.gene31222:intron 25.0%
!!! TTGGAGTTTAATTTTAGAAG+TGG - chr2.4:7033259-7033278 None:intergenic 25.0%
AAATCATTCTTAGTGAACTG+AGG - chr2.4:7034498-7034517 None:intergenic 30.0%
AAGAAGATCTCATATGCAAT+TGG + chr2.4:7035410-7035429 MS.gene31222:CDS 30.0%
AAGTAAATGGCACAACAAAT+TGG - chr2.4:7034933-7034952 None:intergenic 30.0%
AATCATTCTTAGTGAACTGA+GGG - chr2.4:7034497-7034516 None:intergenic 30.0%
AATGCATTAGTGAACTCAAA+CGG - chr2.4:7035139-7035158 None:intergenic 30.0%
AGAATGAATGTGAAATGAAG+AGG - chr2.4:7032602-7032621 None:intergenic 30.0%
ATCCATATTCATTCCAAACA+AGG - chr2.4:7033593-7033612 None:intergenic 30.0%
ATGTTAAAAGATTGGATGCA+AGG + chr2.4:7036138-7036157 MS.gene31222:CDS 30.0%
CACTTCAGATGTTAAAAGAT+TGG + chr2.4:7036130-7036149 MS.gene31222:CDS 30.0%
CAGTTAACCATATAACAACA+GGG - chr2.4:7033491-7033510 None:intergenic 30.0%
CGACATAACAAATTGTTCAA+TGG - chr2.4:7033419-7033438 None:intergenic 30.0%
CTTCACTATCAACCAAATAT+GGG - chr2.4:7035762-7035781 None:intergenic 30.0%
GTCAGGTCAAAAAGAATTTA+AGG + chr2.4:7035723-7035742 MS.gene31222:CDS 30.0%
TAATCAATGTGCTTCACTTA+AGG - chr2.4:7035578-7035597 None:intergenic 30.0%
TAATGTCATAGTACTGCATT+AGG - chr2.4:7035171-7035190 None:intergenic 30.0%
TCCATATTCATTCCAAACAA+GGG - chr2.4:7033592-7033611 None:intergenic 30.0%
TCTCTAAATTTCACAATGAC+AGG + chr2.4:7034455-7034474 MS.gene31222:intron 30.0%
TCTTCACTATCAACCAAATA+TGG - chr2.4:7035763-7035782 None:intergenic 30.0%
TCTTGCAATAATGACATGTT+TGG + chr2.4:7033686-7033705 MS.gene31222:intron 30.0%
TGATGTTTACATGTCACTAT+CGG + chr2.4:7035983-7036002 MS.gene31222:intron 30.0%
TGGATGAAAAATCTAGAAGT+GGG + chr2.4:7035337-7035356 MS.gene31222:CDS 30.0%
TGTTAAAAGATTGGATGCAA+GGG + chr2.4:7036139-7036158 MS.gene31222:CDS 30.0%
TTACAAAAGACAGACACATT+TGG - chr2.4:7032572-7032591 None:intergenic 30.0%
TTCACTATCAACCAAATATG+GGG - chr2.4:7035761-7035780 None:intergenic 30.0%
TTGGATGAAAAATCTAGAAG+TGG + chr2.4:7035336-7035355 MS.gene31222:CDS 30.0%
TTGTTATATGGTTAACTGGA+GGG + chr2.4:7033493-7033512 MS.gene31222:CDS 30.0%
! AATGATCCTCATTTTGTTCA+TGG + chr2.4:7032753-7032772 MS.gene31222:CDS 30.0%
! CTTCAAACTAAAAGTGTGTT+TGG + chr2.4:7035886-7035905 MS.gene31222:intron 30.0%
! TCTAATCAGAATTTGAAGTC+CGG + chr2.4:7035534-7035553 MS.gene31222:intron 30.0%
! TGTTTGGAATGAATATGGAT+GGG + chr2.4:7033593-7033612 MS.gene31222:CDS 30.0%
! TTGTTTGGAATGAATATGGA+TGG + chr2.4:7033592-7033611 MS.gene31222:CDS 30.0%
!! AATAAACAGGGTTTTGCTAT+TGG + chr2.4:7034720-7034739 MS.gene31222:intron 30.0%
!! AGACAATGGACTGATTAATA+AGG + chr2.4:7034797-7034816 MS.gene31222:CDS 30.0%
!! TTTGATTTGTTAGGCACTAA+AGG + chr2.4:7034986-7035005 MS.gene31222:intron 30.0%
!!! TTTTAAGTTTGTTCCCTTCT+CGG + chr2.4:7034888-7034907 MS.gene31222:intron 30.0%
ACAAAATGAGGATCATTCTC+TGG - chr2.4:7032750-7032769 None:intergenic 35.0%
AGGAGAATGTCATTGTTTGT+GGG - chr2.4:7032639-7032658 None:intergenic 35.0%
AGTAATATGTGTTAGTCCTC+CGG + chr2.4:7035451-7035470 MS.gene31222:intron 35.0%
ATAAGAACTTGTCCCTTGTT+TGG + chr2.4:7033577-7033596 MS.gene31222:CDS 35.0%
ATCATTCTCTGGTGTGTTTA+TGG - chr2.4:7032739-7032758 None:intergenic 35.0%
CAAAAGGCTATAGAATCTTG+TGG + chr2.4:7034858-7034877 MS.gene31222:CDS 35.0%
CAAGCAGAAAATCTCATAAG+AGG + chr2.4:7032690-7032709 MS.gene31222:CDS 35.0%
CCAGTTAACCATATAACAAC+AGG - chr2.4:7033492-7033511 None:intergenic 35.0%
CCATTGTCTAATGCAAAATC+TGG - chr2.4:7034786-7034805 None:intergenic 35.0%
CCTGTTGTTATATGGTTAAC+TGG + chr2.4:7033489-7033508 MS.gene31222:CDS 35.0%
CTTGAACATGAAGACAGTTA+GGG + chr2.4:7035243-7035262 MS.gene31222:CDS 35.0%
CTTTGAATCTCGAAACACTA+AGG + chr2.4:7033461-7033480 MS.gene31222:CDS 35.0%
GACATTAGAAAGCAATGTGT+GGG + chr2.4:7035184-7035203 MS.gene31222:CDS 35.0%
GACGAATTATTTAAGAGAGC+CGG - chr2.4:7035556-7035575 None:intergenic 35.0%
GTTGTTATATGGTTAACTGG+AGG + chr2.4:7033492-7033511 MS.gene31222:CDS 35.0%
TATGCCTTGTACTGAATTTC+CGG - chr2.4:7034762-7034781 None:intergenic 35.0%
TCCTCAAGGTTTGTGTTTAA+GGG + chr2.4:7035818-7035837 MS.gene31222:intron 35.0%
TCTTGAACATGAAGACAGTT+AGG + chr2.4:7035242-7035261 MS.gene31222:CDS 35.0%
TGACATTAGAAAGCAATGTG+TGG + chr2.4:7035183-7035202 MS.gene31222:CDS 35.0%
TGATTGAACATAGCACAACA+GGG - chr2.4:7034003-7034022 None:intergenic 35.0%
TTCCTCAAGGTTTGTGTTTA+AGG + chr2.4:7035817-7035836 MS.gene31222:intron 35.0%
TTGATTGAACATAGCACAAC+AGG - chr2.4:7034004-7034023 None:intergenic 35.0%
TTGTTATGACTTCTCCAATG+CGG + chr2.4:7035213-7035232 MS.gene31222:CDS 35.0%
TTTCAGTTTATGACCGAGAA+GGG - chr2.4:7034904-7034923 None:intergenic 35.0%
! ACAATGACAGGCATTTTTCA+AGG + chr2.4:7034467-7034486 MS.gene31222:intron 35.0%
! CACAAGATTCTATAGCCTTT+TGG - chr2.4:7034860-7034879 None:intergenic 35.0%
! CCAGATTTTGCATTAGACAA+TGG + chr2.4:7034783-7034802 MS.gene31222:CDS 35.0%
! CTTGATTGTTTGAGTTTGTG+TGG - chr2.4:7034080-7034099 None:intergenic 35.0%
! GGTGTGTTTATGGAATCTTT+TGG - chr2.4:7032729-7032748 None:intergenic 35.0%
! TCCCTTGTTTGGAATGAATA+TGG + chr2.4:7033588-7033607 MS.gene31222:CDS 35.0%
! TCTATTTTACCTTGCAGCTT+CGG - chr2.4:7032880-7032899 None:intergenic 35.0%
! TGGTCCTTTTAAATTCTCCA+AGG + chr2.4:7033554-7033573 MS.gene31222:CDS 35.0%
! TTTTAATAGGTGAAAGAGGC+TGG + chr2.4:7036075-7036094 MS.gene31222:intron 35.0%
! TTTTCAGTTTATGACCGAGA+AGG - chr2.4:7034905-7034924 None:intergenic 35.0%
!! CAATTTGTTATGTCGATTGC+AGG + chr2.4:7033424-7033443 MS.gene31222:intron 35.0%
AAATGTGATTCTCGTTTGGC+AGG + chr2.4:7035670-7035689 MS.gene31222:intron 40.0%
AAGACAGTTAGGGAAGCTTT+AGG + chr2.4:7035253-7035272 MS.gene31222:CDS 40.0%
AAGATGATGCAGTTGAAGAG+AGG - chr2.4:7032659-7032678 None:intergenic 40.0%
AAGGGAAACTGATAAGGACA+AGG + chr2.4:7036157-7036176 MS.gene31222:CDS 40.0%
AAGGGACAAGTTCTTATCCT+TGG - chr2.4:7033574-7033593 None:intergenic 40.0%
AATGTGATTCTCGTTTGGCA+GGG + chr2.4:7035671-7035690 MS.gene31222:intron 40.0%
ACCCTTAAACACAAACCTTG+AGG - chr2.4:7035822-7035841 None:intergenic 40.0%
AGCACAACAGGGAAAATCTA+GGG - chr2.4:7033992-7034011 None:intergenic 40.0%
AGGATGACCCTGTTGTTATA+TGG + chr2.4:7033481-7033500 MS.gene31222:CDS 40.0%
AGGGAAACTGATAAGGACAA+GGG + chr2.4:7036158-7036177 MS.gene31222:CDS 40.0%
ATATTTGTAGACCAACCCAC+TGG + chr2.4:7034176-7034195 MS.gene31222:CDS 40.0%
CAAAAGACAGACACATTTGG+AGG - chr2.4:7032569-7032588 None:intergenic 40.0%
CAAGGAAACAAGGCAAAAGA+AGG + chr2.4:7034579-7034598 MS.gene31222:CDS 40.0%
CAATCCGGAAATTCAGTACA+AGG + chr2.4:7034755-7034774 MS.gene31222:CDS 40.0%
CGATCTATATGACTTCTTGC+AGG + chr2.4:7034262-7034281 MS.gene31222:CDS 40.0%
CTTGTACTGAATTTCCGGAT+TGG - chr2.4:7034757-7034776 None:intergenic 40.0%
GAAAAGAAGTTCACTTTCCC+TGG + chr2.4:7032786-7032805 MS.gene31222:CDS 40.0%
GAATGAATATGGATGGGACA+TGG + chr2.4:7033599-7033618 MS.gene31222:CDS 40.0%
GAGGAGAATGTCATTGTTTG+TGG - chr2.4:7032640-7032659 None:intergenic 40.0%
GATCTCATATGCAATTGGCT+TGG + chr2.4:7035415-7035434 MS.gene31222:CDS 40.0%
GTTCAAGAATGTTTCCGCAT+TGG - chr2.4:7035230-7035249 None:intergenic 40.0%
TAGCACAACAGGGAAAATCT+AGG - chr2.4:7033993-7034012 None:intergenic 40.0%
TAGGGTTCATCAAGGAAACA+AGG + chr2.4:7034569-7034588 MS.gene31222:CDS 40.0%
TCAACCAAATATGGGGTTGT+AGG - chr2.4:7035754-7035773 None:intergenic 40.0%
TCATTGTTTGTGGGATAAGC+TGG - chr2.4:7032630-7032649 None:intergenic 40.0%
TCTTCTTGAGGATGGAATCA+AGG + chr2.4:7035369-7035388 MS.gene31222:CDS 40.0%
TTTCATCCAATCCTGCAACA+TGG - chr2.4:7035326-7035345 None:intergenic 40.0%
! ACATTCCAGCTTTTGCATCT+AGG + chr2.4:7034550-7034569 MS.gene31222:CDS 40.0%
! CATTCCAGCTTTTGCATCTA+GGG + chr2.4:7034551-7034570 MS.gene31222:CDS 40.0%
! TTGTTTGAGTTTGTGTGGAC+TGG - chr2.4:7034075-7034094 None:intergenic 40.0%
! TTTGCATCTAGGGTTCATCA+AGG + chr2.4:7034561-7034580 MS.gene31222:CDS 40.0%
! TTTTTGACCTGACCATGTCA+TGG - chr2.4:7035716-7035735 None:intergenic 40.0%
!! AACATCTGAAGTGCAGCTTT+TGG - chr2.4:7036123-7036142 None:intergenic 40.0%
!! GATCAGATAATGGACATAGC+CGG + chr2.4:7035055-7035074 MS.gene31222:CDS 40.0%
!! GATTTGTTAGGCACTAAAGG+TGG + chr2.4:7034989-7035008 MS.gene31222:intron 40.0%
!! TGGGAACAACTTGTTGATGT+TGG - chr2.4:7034832-7034851 None:intergenic 40.0%
!! TGGTAACGGATTAACCAATC+CGG + chr2.4:7034740-7034759 MS.gene31222:CDS 40.0%
!! TTAGGGAAGCTTTAGGTGTT+GGG + chr2.4:7035260-7035279 MS.gene31222:CDS 40.0%
!! TTGGTTGATAGTGAAGAAGC+AGG + chr2.4:7035766-7035785 MS.gene31222:CDS 40.0%
!!! CAGGGTTTTGCTATTGGTAA+CGG + chr2.4:7034726-7034745 MS.gene31222:intron 40.0%
AAACTGATAAGGACAAGGGG+AGG + chr2.4:7036162-7036181 MS.gene31222:CDS 45.0%
AGGGAAAATCTAGGGGGTTT+AGG - chr2.4:7033984-7034003 None:intergenic 45.0%
AGTATACAGTGCCATGTTGC+AGG + chr2.4:7035312-7035331 MS.gene31222:CDS 45.0%
ATAGCTGAAACCTGTTCCAG+TGG - chr2.4:7034195-7034214 None:intergenic 45.0%
ATGTCCAGCGTATGATTCTC+CGG - chr2.4:7034529-7034548 None:intergenic 45.0%
ATTACCGGAGAATCATACGC+TGG + chr2.4:7034522-7034541 MS.gene31222:CDS 45.0%
ATTCCATCCTCAAGAAGAGC+AGG - chr2.4:7035367-7035386 None:intergenic 45.0%
ATTCCTGCTCTTCTTGAGGA+TGG + chr2.4:7035361-7035380 MS.gene31222:CDS 45.0%
CAAACCTTGAGGAAAGCAAG+AGG - chr2.4:7035811-7035830 None:intergenic 45.0%
CACAACAGGGAAAATCTAGG+GGG - chr2.4:7033990-7034009 None:intergenic 45.0%
CACCAGAGTCAACAAAACCA+GGG - chr2.4:7032806-7032825 None:intergenic 45.0%
GAAGAACTTGGTCATCATGC+AGG + chr2.4:7032834-7032853 MS.gene31222:CDS 45.0%
GACGTTCGACATGATGAAGA+CGG + chr2.4:7034230-7034249 MS.gene31222:CDS 45.0%
GCACAACAGGGAAAATCTAG+GGG - chr2.4:7033991-7034010 None:intergenic 45.0%
GGATGCAAGGGAAACTGATA+AGG + chr2.4:7036151-7036170 MS.gene31222:CDS 45.0%
GGGAAACTGATAAGGACAAG+GGG + chr2.4:7036159-7036178 MS.gene31222:CDS 45.0%
GGTGCTTCTGTTGAAGAACT+TGG + chr2.4:7032822-7032841 MS.gene31222:CDS 45.0%
GTCTCCTACAACCCCATATT+TGG + chr2.4:7035747-7035766 MS.gene31222:CDS 45.0%
TAGCTGAAACCTGTTCCAGT+GGG - chr2.4:7034194-7034213 None:intergenic 45.0%
TAGGGGGTTTAGGCCATATT+TGG - chr2.4:7033974-7033993 None:intergenic 45.0%
TGAACCCTAGATGCAAAAGC+TGG - chr2.4:7034558-7034577 None:intergenic 45.0%
! ATCAAGGTGCTTGTGTATGC+TGG + chr2.4:7035385-7035404 MS.gene31222:CDS 45.0%
! CTATAGCCTTTTGGCATCGT+GGG - chr2.4:7034851-7034870 None:intergenic 45.0%
! TCTATAGCCTTTTGGCATCG+TGG - chr2.4:7034852-7034871 None:intergenic 45.0%
!! CAATAGCTACAAAGCACCGA+CGG - chr2.4:7036030-7036049 None:intergenic 45.0%
!! GCAGCTTTTGGTTGATCCAT+AGG - chr2.4:7036111-7036130 None:intergenic 45.0%
!! GTTAGGGAAGCTTTAGGTGT+TGG + chr2.4:7035259-7035278 MS.gene31222:CDS 45.0%
!! TAGGGAAGCTTTAGGTGTTG+GGG + chr2.4:7035261-7035280 MS.gene31222:CDS 45.0%
!!! GCAGGAGTTTTGAAAAGCCA+CGG + chr2.4:7035784-7035803 MS.gene31222:CDS 45.0%
!!! TTCCCTGGTTTTGTTGACTC+TGG + chr2.4:7032801-7032820 MS.gene31222:CDS 45.0%
ACAGTGCCATGTTGCAGGAT+TGG + chr2.4:7035317-7035336 MS.gene31222:CDS 50.0%
ATATGGTTAACTGGAGGGCC+AGG + chr2.4:7033498-7033517 MS.gene31222:CDS 50.0%
CTTACTTTCCAGCCAAACGC+TGG - chr2.4:7035497-7035516 None:intergenic 50.0%
GCAATTTCACTGCTGCATCC+TGG - chr2.4:7033519-7033538 None:intergenic 50.0%
GCACCAGAGTCAACAAAACC+AGG - chr2.4:7032807-7032826 None:intergenic 50.0%
GGGTATTCCTGCTCTTCTTG+AGG + chr2.4:7035357-7035376 MS.gene31222:CDS 50.0%
GTTGATGCCATGACATGGTC+AGG + chr2.4:7035706-7035725 MS.gene31222:CDS 50.0%
GTTGTTCCCACGATGCCAAA+AGG + chr2.4:7034842-7034861 MS.gene31222:CDS 50.0%
TACCTTGCAGCTTCGGAATG+AGG - chr2.4:7032873-7032892 None:intergenic 50.0%
TCGAACGTCGCTATCATCAG+TGG - chr2.4:7034219-7034238 None:intergenic 50.0%
TGAAACCTGTTCCAGTGGGT+TGG - chr2.4:7034190-7034209 None:intergenic 50.0%
TGTTAGTCCTCCGGTTTCCA+AGG + chr2.4:7035460-7035479 MS.gene31222:intron 50.0%
TTACCCGGTTCCTTGGAAAC+CGG - chr2.4:7035473-7035492 None:intergenic 50.0%
TTCCTCATTCCGAAGCTGCA+AGG + chr2.4:7032868-7032887 MS.gene31222:CDS 50.0%
TTTGGCAGGGAATTCGAGGT+GGG + chr2.4:7035684-7035703 MS.gene31222:intron 50.0%
!! AGCTTTAGGTGTTGGGGACT+TGG + chr2.4:7035267-7035286 MS.gene31222:CDS 50.0%
!! GTCACTATCGGTGTTGTGTC+TGG + chr2.4:7035995-7036014 MS.gene31222:intron 50.0%
CAGCCAAACGCTGGATTACC+CGG - chr2.4:7035488-7035507 None:intergenic 55.0%
CGGACCTCTTGCTTTCCTCA+AGG + chr2.4:7035804-7035823 MS.gene31222:CDS 55.0%
CTCGTTTGGCAGGGAATTCG+AGG + chr2.4:7035680-7035699 MS.gene31222:intron 55.0%
GAACCGGGTAATCCAGCGTT+TGG + chr2.4:7035482-7035501 MS.gene31222:intron 55.0%
GTAGACCAACCCACTGGAAC+AGG + chr2.4:7034182-7034201 MS.gene31222:CDS 55.0%
GTTTGGCAGGGAATTCGAGG+TGG + chr2.4:7035683-7035702 MS.gene31222:intron 55.0%
TCCTCCGGTTTCCAAGGAAC+CGG + chr2.4:7035466-7035485 MS.gene31222:intron 55.0%
TGAGGAAAGCAAGAGGTCCG+TGG - chr2.4:7035804-7035823 None:intergenic 55.0%
TGTCTGGCACAAGTGTCCGT+CGG + chr2.4:7036011-7036030 MS.gene31222:intron 55.0%
! GGCTGGTCACATGGTTCCTA+TGG + chr2.4:7036092-7036111 MS.gene31222:CDS 55.0%
! GGTGAAAGAGGCTGGTCACA+TGG + chr2.4:7036083-7036102 MS.gene31222:intron 55.0%
!! GGTGGGTTGATGCCATGACA+TGG + chr2.4:7035701-7035720 MS.gene31222:CDS 55.0%
CCTCCGGTTTCCAAGGAACC+GGG + chr2.4:7035467-7035486 MS.gene31222:intron 60.0%
CGCTGGATTACCCGGTTCCT+TGG - chr2.4:7035480-7035499 None:intergenic 60.0%
CGGGTAATCCAGCGTTTGGC+TGG + chr2.4:7035486-7035505 MS.gene31222:intron 60.0%
CCCGGTTCCTTGGAAACCGG+AGG - chr2.4:7035470-7035489 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 7032546 7036206 7032546 ID=MS.gene31222
chr2.4 mRNA 7032546 7036206 7032546 ID=MS.gene31222.t1;Parent=MS.gene31222
chr2.4 exon 7032546 7032889 7032546 ID=MS.gene31222.t1.exon1;Parent=MS.gene31222.t1
chr2.4 CDS 7032546 7032889 7032546 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
chr2.4 exon 7033446 7033620 7033446 ID=MS.gene31222.t1.exon2;Parent=MS.gene31222.t1
chr2.4 CDS 7033446 7033620 7033446 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
chr2.4 exon 7034164 7034283 7034164 ID=MS.gene31222.t1.exon3;Parent=MS.gene31222.t1
chr2.4 CDS 7034164 7034283 7034164 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
chr2.4 exon 7034477 7034620 7034477 ID=MS.gene31222.t1.exon4;Parent=MS.gene31222.t1
chr2.4 CDS 7034477 7034620 7034477 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
chr2.4 exon 7034729 7034879 7034729 ID=MS.gene31222.t1.exon5;Parent=MS.gene31222.t1
chr2.4 CDS 7034729 7034879 7034729 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
chr2.4 exon 7034999 7035087 7034999 ID=MS.gene31222.t1.exon6;Parent=MS.gene31222.t1
chr2.4 CDS 7034999 7035087 7034999 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
chr2.4 exon 7035178 7035436 7035178 ID=MS.gene31222.t1.exon7;Parent=MS.gene31222.t1
chr2.4 CDS 7035178 7035436 7035178 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
chr2.4 exon 7035692 7035825 7035692 ID=MS.gene31222.t1.exon8;Parent=MS.gene31222.t1
chr2.4 CDS 7035692 7035825 7035692 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
chr2.4 exon 7036084 7036206 7036084 ID=MS.gene31222.t1.exon9;Parent=MS.gene31222.t1
chr2.4 CDS 7036084 7036206 7036084 ID=cds.MS.gene31222.t1;Parent=MS.gene31222.t1
Gene Sequence

>MS.gene31222

ATGGCATCATCTTTAAGGTTCCTCCAAATGTGTCTGTCTTTTGTAATGTTCTTCCTCTTCATTTCACATTCATTCTCATCTCCAGCTTATCCCACAAACAATGACATTCTCCTCTCTTCAACTGCATCATCTTACCCAAAATTACAAGCAGAAAATCTCATAAGAGGACTTAATCTTTTTCCAAAAGATTCCATAAACACACCAGAGAATGATCCTCATTTTGTTCATGGAAATATTGTGGAAAAGAAGTTCACTTTCCCTGGTTTTGTTGACTCTGGTGCTTCTGTTGAAGAACTTGGTCATCATGCAGGCTATTATAGTCTTCCTCATTCCGAAGCTGCAAGGTAAAATAGATCATGACTATCTTTGAATTTTAGGCCAAAATAAGATAAAATAGAATGAAATATTAGGTAAGTTTTTTGTGGTAAAAAATGATCTAAGCATAAAGCAAGAAAAGCTTTAGACAGAATAAAAATATAGTTCTTGGAAAGCATAAAGTCAGAATAATAAAAGTGAAAGTGAACAAGCAAAGAATTTATAAAAAATGGAATAGTTTGGATTTCTTATGTTCATTTGATCTAGTTTAATTTATTGGATATTTGTTTGATCATGTATTTTTATCATGTCTTTGATGTTGTCATTACTCGTGTATCAACTTAATCAAAATATATATCAAAGTATCATAATTTGAAATATCTGTTATAACATCTCCACTTCTAAAATTAAACTCCAAAATAGACCGAATTTTAAGAAAAACACTTTTTCAAATTATTACTTAACCAAAATAAGAATTTGATGACAACTCTATTGATTTATATTAATTGGTTTTTTGTCAACTCACATCATAATAATGTGTGCGAAGACATGTTTCCATTGAACAATTTGTTATGTCGATTGCAGGATGTTCTATTTTTTCTTTGAATCTCGAAACACTAAGGATGACCCTGTTGTTATATGGTTAACTGGAGGGCCAGGATGCAGCAGTGAAATTGCTATGTTTTATGAAAATGGTCCTTTTAAATTCTCCAAGGATAAGAACTTGTCCCTTGTTTGGAATGAATATGGATGGGACATGGTACCTATTAAATTATTCTCTTTTCTTTTTCTTTTTCCACTTTAGTTCTTACTCATATATGATCATCTTGCAATAATGACATGTTTGGATTGACTTATTTGCGCTTATTTGCTGACATAAACAATTGTGAGACTGTTTGAGAATTTTTTTGGAAACAACTTATGTCATATTTGTAGGTTTTTTTGAGAGGTCATATCTATAAGTTGTTTTCAGTTTATTTTCATAAGCTCCAAACTAATATAGCTTATGAAACTGTTTGACTTTATTTACCTTTTATTGTAAAAATAGCTAATACATAAGCATTTATATGACAAGGGCTTATGATATAAGTGCTTAATTAAGTTGTTTATCCAAATATGGCCTAAACCCCCTAGATTTTCCCTGTTGTGCTATGTTCAATCAATGTAGAGTGTCAACAACAATAGCAAAAGTAATGATATTTATGCATATACCAGTCCACACAAACTCAAACAATCAAGTTATTATTTATTAGCACTGAAGTTGTTTTTTTAATAGTGCCAATGCATTTTTTCTTGTTTGTAGGCATCAAATATTATATTTGTAGACCAACCCACTGGAACAGGTTTCAGCTATACCACTGATGATAGCGACGTTCGACATGATGAAGACGGTGTTAGCAACGATCTATATGACTTCTTGCAGGTTATTTGATTTATTCAGTGATTAAAGAAAATAATATCAAAATAACATTTGAAATGATTGCTTTATTTATTGAATTATTCACTTGTATTAAGTGGTTAAACTTGATTCACATTACAATCAATCTAAATTGCAAATTCTATGTTAAACTCTAATGTTGTGCATACATAAGATTCTCTAAATTTCACAATGACAGGCATTTTTCAAGGAACACCCTCAGTTCACTAAGAATGATTTTTATATTACCGGAGAATCATACGCTGGACATTACATTCCAGCTTTTGCATCTAGGGTTCATCAAGGAAACAAGGCAAAAGAAGGAATTCATATAAATCTAAAGGTAATATTACATAGTCATATAATGTACACTATATTTATGTATAGGTAAACTTTGTCAAAATAATATCAATGTAATGTAAACTAATTTGTAAACCTTTATAATAAACAGGGTTTTGCTATTGGTAACGGATTAACCAATCCGGAAATTCAGTACAAGGCATATCCAGATTTTGCATTAGACAATGGACTGATTAATAAGGATGAACATGCCAACATCAACAAGTTGTTCCCACGATGCCAAAAGGCTATAGAATCTTGTGGTATGACATTTTAAGTTTGTTCCCTTCTCGGTCATAAACTGAAAATTTTACCAATTTGTTGTGCCATTTACTTGTTATTTTTCCTTATTGACACTAATAGTTTGTATTTGATTTGTTAGGCACTAAAGGTGGAGACGCATGCATGACTTCAAGAAGCATTTGCAGTGAAATATTCGATCAGATAATGGACATAGCCGGCAACATTAATGTATGATATCAAAAAAGAAAAGCACTATAAGTATATATGAAAATAATGCCGTTTGAGTTCACTAATGCATTATGTTTATTCCTAATGCAGTACTATGACATTAGAAAGCAATGTGTGGGAAGTCTTTGTTATGACTTCTCCAATGCGGAAACATTCTTGAACATGAAGACAGTTAGGGAAGCTTTAGGTGTTGGGGACTTGGAATTTGTTTCTTGCAGTAGCACAGTATACAGTGCCATGTTGCAGGATTGGATGAAAAATCTAGAAGTGGGTATTCCTGCTCTTCTTGAGGATGGAATCAAGGTGCTTGTGTATGCTGGAGAAGAAGATCTCATATGCAATTGGCTTGGTAAGCTCAATTACAGTAATATGTGTTAGTCCTCCGGTTTCCAAGGAACCGGGTAATCCAGCGTTTGGCTGGAAAGTAAGTAAAGTTCTGCAATAGATCTAATCAGAATTTGAAGTCCGGCTCTCTTAAATAATTCGTCCTTAAGTGAAGCACATTGATTATAAGTTTGACTAATTTGGAACCAAAATTTCTTGTGTCAAAATAGAATACACAAATATGGTAGTTAGATTTATAAATGTGATTCTCGTTTGGCAGGGAATTCGAGGTGGGTTGATGCCATGACATGGTCAGGTCAAAAAGAATTTAAGGCGTCTCCTACAACCCCATATTTGGTTGATAGTGAAGAAGCAGGAGTTTTGAAAAGCCACGGACCTCTTGCTTTCCTCAAGGTTTGTGTTTAAGGGTGTATTCATTAGAGTATTAGACGATTGATCTATATAGCACTGACACTTCAAACTAAAAGTGTGTTTGGTTTTCGACGCACGCTGTGTTCGACATCGAGACAAGTATTTGCATTTGATCATTTTTATTTTCTCAAATTATTACTGATGTTTACATGTCACTATCGGTGTTGTGTCTGGCACAAGTGTCCGTCGGTGCTTTGTAGCTATTGATACGCGTGTGTTTAACTTCCGTTTTTTTAATAGGTGAAAGAGGCTGGTCACATGGTTCCTATGGATCAACCAAAAGCTGCACTTCAGATGTTAAAAGATTGGATGCAAGGGAAACTGATAAGGACAAGGGGAGGAGATAATGTCTCGTCTATATGA
Protein sequence

>MS.gene31222.t1

MASSLRFLQMCLSFVMFFLFISHSFSSPAYPTNNDILLSSTASSYPKLQAENLIRGLNLFPKDSINTPENDPHFVHGNIVEKKFTFPGFVDSGASVEELGHHAGYYSLPHSEAARMFYFFFESRNTKDDPVVIWLTGGPGCSSEIAMFYENGPFKFSKDKNLSLVWNEYGWDMASNIIFVDQPTGTGFSYTTDDSDVRHDEDGVSNDLYDFLQAFFKEHPQFTKNDFYITGESYAGHYIPAFASRVHQGNKAKEGIHINLKGFAIGNGLTNPEIQYKAYPDFALDNGLINKDEHANINKLFPRCQKAIESCGTKGGDACMTSRSICSEIFDQIMDIAGNINYYDIRKQCVGSLCYDFSNAETFLNMKTVREALGVGDLEFVSCSSTVYSAMLQDWMKNLEVGIPALLEDGIKVLVYAGEEDLICNWLGNSRWVDAMTWSGQKEFKASPTTPYLVDSEEAGVLKSHGPLAFLKVKEAGHMVPMDQPKAALQMLKDWMQGKLIRTRGGDNVSSI