Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31950.t1 | XP_013457066.1 | 89.1 | 184 | 19 | 1 | 1 | 183 | 123 | 306 | 2.40E-73 | 285 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31950.t1 | Q7XKE9 | 69.9 | 123 | 36 | 1 | 1 | 123 | 133 | 254 | 1.1e-42 | 174.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31950.t1 | G7ZZ83 | 89.1 | 184 | 19 | 1 | 1 | 183 | 123 | 306 | 1.7e-73 | 285.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050170 | MS.gene31950 | -0.808233 | 3.43E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene31950 | -0.801258 | 9.80E-49 | -1.69E-46 |
| MS.gene050864 | MS.gene31950 | 0.829496 | 4.98E-55 | -1.69E-46 |
| MS.gene050904 | MS.gene31950 | -0.802991 | 4.31E-49 | -1.69E-46 |
| MS.gene052494 | MS.gene31950 | 0.955223 | 4.97E-113 | -1.69E-46 |
| MS.gene053483 | MS.gene31950 | -0.807585 | 4.70E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene31950 | -0.803996 | 2.67E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene31950 | -0.820949 | 5.23E-53 | -1.69E-46 |
| MS.gene05960 | MS.gene31950 | 0.80251 | 5.42E-49 | -1.69E-46 |
| MS.gene06042 | MS.gene31950 | 0.839317 | 1.70E-57 | -1.69E-46 |
| MS.gene060982 | MS.gene31950 | -0.806452 | 8.17E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31950.t1 | MTR_4g088980 | 97.283 | 184 | 4 | 1 | 1 | 183 | 123 | 306 | 6.12e-127 | 359 |
| MS.gene31950.t1 | MTR_2g013220 | 74.457 | 184 | 39 | 2 | 1 | 183 | 137 | 313 | 4.17e-84 | 251 |
| MS.gene31950.t1 | MTR_8g098515 | 58.673 | 196 | 68 | 4 | 1 | 183 | 151 | 346 | 8.41e-57 | 182 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31950.t1 | AT2G20760 | 56.477 | 193 | 68 | 4 | 1 | 179 | 132 | 322 | 6.32e-54 | 174 |
| MS.gene31950.t1 | AT2G40060 | 63.158 | 114 | 40 | 1 | 4 | 115 | 115 | 228 | 7.60e-47 | 154 |
| MS.gene31950.t1 | AT3G51890 | 60.163 | 123 | 46 | 1 | 1 | 123 | 121 | 240 | 8.47e-46 | 151 |
Find 60 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCCTTTGGTTGTGATTC+AGG | 0.112857 | 4.1:-20809383 | None:intergenic |
| GTTTGCCTGGCTTTGGGCCT+TGG | 0.150519 | 4.1:-20809181 | None:intergenic |
| CGGTGGGTTTGCCTGGCTTT+GGG | 0.248540 | 4.1:-20809187 | None:intergenic |
| GCAAACCCACCGACCTTTCT+AGG | 0.257216 | 4.1:+20809198 | MS.gene31950:CDS |
| TCGGTGGGTTTGCCTGGCTT+TGG | 0.260717 | 4.1:-20809188 | None:intergenic |
| TTTGTGAAAATTCTCTTGAT+TGG | 0.330165 | 4.1:-20809042 | None:intergenic |
| TGAATCTACAGTACCGTTAT+TGG | 0.331003 | 4.1:-20809408 | None:intergenic |
| GGTGCTGATCCATTTGGTTT+AGG | 0.333375 | 4.1:-20809350 | None:intergenic |
| TGAGAAGAAAAGAGGCAAAA+AGG | 0.365407 | 4.1:+20809127 | MS.gene31950:CDS |
| AAAATTACTGGAAAGCAATT+GGG | 0.366531 | 4.1:+20809075 | MS.gene31950:CDS |
| TGCCTGGCTTTGGGCCTTGG+AGG | 0.380338 | 4.1:-20809178 | None:intergenic |
| TTCCCTTTGGAGTCTTTAGC+AGG | 0.387984 | 4.1:-20809287 | None:intergenic |
| CCGTTATTGGCAGCATCCTT+TGG | 0.394987 | 4.1:-20809395 | None:intergenic |
| GGTTTAGGTGCTGATCCATT+TGG | 0.396493 | 4.1:-20809335 | None:intergenic |
| GAAAGGTCGGTGGGTTTGCC+TGG | 0.402706 | 4.1:-20809194 | None:intergenic |
| ACTTCCGGTGCTGATCCATT+TGG | 0.420411 | 4.1:-20809356 | None:intergenic |
| TTGGAGTCTTTAGCAGGTGC+AGG | 0.422981 | 4.1:-20809281 | None:intergenic |
| TGTGATTCAGGTGCAACTTC+CGG | 0.424313 | 4.1:-20809371 | None:intergenic |
| CTCAATGTTGGCAACCTCAC+GGG | 0.426203 | 4.1:-20809108 | None:intergenic |
| GCAGACTGCTCCTCAGTGGC+AGG | 0.431231 | 4.1:-20809446 | None:intergenic |
| GTTTGTGGAACAGTTATACT+TGG | 0.453267 | 4.1:+20809019 | MS.gene31950:intron |
| ACAAGGTTCAAAACAGAGAA+AGG | 0.459770 | 4.1:+20808908 | MS.gene31950:CDS |
| AATGAGATTAAAGATTATTG+AGG | 0.464614 | 4.1:+20808828 | None:intergenic |
| GAAACAGCAACTAAACCAAA+TGG | 0.475248 | 4.1:+20809320 | MS.gene31950:CDS |
| GGATCAGCACCTAAACCAAA+TGG | 0.488856 | 4.1:+20809341 | MS.gene31950:CDS |
| AAGTGGGTTTCTATGAGAAA+AGG | 0.493819 | 4.1:+20808866 | MS.gene31950:CDS |
| GTGGCAGGAGCTTCTTTCTG+AGG | 0.503664 | 4.1:-20809431 | None:intergenic |
| GTCCTCCAAGGCCCAAAGCC+AGG | 0.509150 | 4.1:+20809176 | MS.gene31950:CDS |
| TTAGCAGGTGCAGGTGGAGG+AGG | 0.510354 | 4.1:-20809272 | None:intergenic |
| CCAAAGGATGCTGCCAATAA+CGG | 0.510873 | 4.1:+20809395 | MS.gene31950:CDS |
| ATATCTGCCGCATCCTAGAA+AGG | 0.513686 | 4.1:-20809211 | None:intergenic |
| TCTTTAGCAGGTGCAGGTGG+AGG | 0.524857 | 4.1:-20809275 | None:intergenic |
| CAAGGTTCAAAACAGAGAAA+GGG | 0.537446 | 4.1:+20808909 | MS.gene31950:CDS |
| TCTCAATGTTGGCAACCTCA+CGG | 0.539692 | 4.1:-20809109 | None:intergenic |
| CACCTGCTAAAGACTCCAAA+GGG | 0.547927 | 4.1:+20809285 | MS.gene31950:CDS |
| GAGTCTTTAGCAGGTGCAGG+TGG | 0.551285 | 4.1:-20809278 | None:intergenic |
| GCCAACATTGAGAAGAAAAG+AGG | 0.561154 | 4.1:+20809119 | MS.gene31950:CDS |
| AAAGATTATTGAGGAAGCTG+AGG | 0.562392 | 4.1:+20808837 | MS.gene31950:CDS |
| GAAGCTGAGGATTACAAAGT+GGG | 0.570600 | 4.1:+20808850 | MS.gene31950:CDS |
| AAATTACTGGAAAGCAATTG+GGG | 0.589625 | 4.1:+20809076 | MS.gene31950:CDS |
| CCTTGGAGGACAGTAACTGA+TGG | 0.599991 | 4.1:-20809164 | None:intergenic |
| CCACCGACCTTTCTAGGATG+CGG | 0.602155 | 4.1:+20809204 | MS.gene31950:CDS |
| TGCACCTGAATCACAACCAA+AGG | 0.604455 | 4.1:+20809379 | MS.gene31950:CDS |
| GGAGGAGGAATCATGTGAAG+TGG | 0.606489 | 4.1:-20809257 | None:intergenic |
| TGCTAAAGACTCCAAAGGGA+AGG | 0.606563 | 4.1:+20809289 | MS.gene31950:CDS |
| CTGCCGCATCCTAGAAAGGT+CGG | 0.609710 | 4.1:-20809207 | None:intergenic |
| GGAAGCTGAGGATTACAAAG+TGG | 0.610216 | 4.1:+20808849 | MS.gene31950:CDS |
| CGCATCCTAGAAAGGTCGGT+GGG | 0.610689 | 4.1:-20809203 | None:intergenic |
| TCAATGTTGGCAACCTCACG+GGG | 0.611587 | 4.1:-20809107 | None:intergenic |
| TCAAAACAGAGAAAGGGAGA+AGG | 0.613489 | 4.1:+20808915 | MS.gene31950:CDS |
| CCATCAGTTACTGTCCTCCA+AGG | 0.615544 | 4.1:+20809164 | MS.gene31950:CDS |
| CCGCATCCTAGAAAGGTCGG+TGG | 0.616621 | 4.1:-20809204 | None:intergenic |
| TAAACCAAATGGATCAGCAC+CGG | 0.618396 | 4.1:+20809352 | MS.gene31950:CDS |
| AAAGACTCCAAAGGGAAGGA+CGG | 0.629704 | 4.1:+20809293 | MS.gene31950:CDS |
| GCACCTGCTAAAGACTCCAA+AGG | 0.630783 | 4.1:+20809284 | MS.gene31950:CDS |
| GGAGGAATCATGTGAAGTGG+TGG | 0.643969 | 4.1:-20809254 | None:intergenic |
| GAAAGAAGCTCCTGCCACTG+AGG | 0.676516 | 4.1:+20809436 | MS.gene31950:CDS |
| TGCAGCAGACTGCTCCTCAG+TGG | 0.680257 | 4.1:-20809450 | None:intergenic |
| TGGGGAGATAATTCCCCGTG+AGG | 0.688947 | 4.1:+20809094 | MS.gene31950:CDS |
| GCTCAATGTTGAGACAAACA+AGG | 0.723788 | 4.1:+20808891 | MS.gene31950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTCTAAAAATTAAAAGAA+CGG | - | chr4.1:20808992-20809011 | None:intergenic | 15.0% |
| !!! | GTAAAAAGTAATGATAATGT+AGG | - | chr4.1:20808954-20808973 | None:intergenic | 20.0% |
| ! | AAAAATTACTGGAAAGCAAT+TGG | + | chr4.1:20809074-20809093 | MS.gene31950:CDS | 25.0% |
| ! | AAAATTACTGGAAAGCAATT+GGG | + | chr4.1:20809075-20809094 | MS.gene31950:CDS | 25.0% |
| ! | TTTGTGAAAATTCTCTTGAT+TGG | - | chr4.1:20809045-20809064 | None:intergenic | 25.0% |
| AAATTACTGGAAAGCAATTG+GGG | + | chr4.1:20809076-20809095 | MS.gene31950:CDS | 30.0% | |
| TTAGAGATGAACTATGTTTG+TGG | + | chr4.1:20809004-20809023 | MS.gene31950:intron | 30.0% | |
| ! | TCAAGAGAATTTTCACAAAG+AGG | + | chr4.1:20809046-20809065 | MS.gene31950:CDS | 30.0% |
| AAAGATTATTGAGGAAGCTG+AGG | + | chr4.1:20808837-20808856 | MS.gene31950:CDS | 35.0% | |
| AAGAGGCTGACAAAAATTAC+TGG | + | chr4.1:20809063-20809082 | MS.gene31950:CDS | 35.0% | |
| ACAAGGTTCAAAACAGAGAA+AGG | + | chr4.1:20808908-20808927 | MS.gene31950:CDS | 35.0% | |
| CAAGGTTCAAAACAGAGAAA+GGG | + | chr4.1:20808909-20808928 | MS.gene31950:CDS | 35.0% | |
| GAAACAGCAACTAAACCAAA+TGG | + | chr4.1:20809320-20809339 | MS.gene31950:CDS | 35.0% | |
| GTTTGTGGAACAGTTATACT+TGG | + | chr4.1:20809019-20809038 | MS.gene31950:intron | 35.0% | |
| TGAATCTACAGTACCGTTAT+TGG | - | chr4.1:20809411-20809430 | None:intergenic | 35.0% | |
| TGAGAAGAAAAGAGGCAAAA+AGG | + | chr4.1:20809127-20809146 | MS.gene31950:CDS | 35.0% | |
| ! | AAGTGGGTTTCTATGAGAAA+AGG | + | chr4.1:20808866-20808885 | MS.gene31950:CDS | 35.0% |
| GAAAAGAGGCAAAAAGGATC+AGG | + | chr4.1:20809133-20809152 | MS.gene31950:CDS | 40.0% | |
| GAAGCTGAGGATTACAAAGT+GGG | + | chr4.1:20808850-20808869 | MS.gene31950:CDS | 40.0% | |
| GCCAACATTGAGAAGAAAAG+AGG | + | chr4.1:20809119-20809138 | MS.gene31950:CDS | 40.0% | |
| GCTCAATGTTGAGACAAACA+AGG | + | chr4.1:20808891-20808910 | MS.gene31950:CDS | 40.0% | |
| TAAACCAAATGGATCAGCAC+CGG | + | chr4.1:20809331-20809350 | MS.gene31950:CDS | 40.0% | |
| TCAAAACAGAGAAAGGGAGA+AGG | + | chr4.1:20808915-20808934 | MS.gene31950:CDS | 40.0% | |
| ! | GCCTCTTTTCTTCTCAATGT+TGG | - | chr4.1:20809123-20809142 | None:intergenic | 40.0% |
| AAAGACTCCAAAGGGAAGGA+CGG | + | chr4.1:20809293-20809312 | MS.gene31950:CDS | 45.0% | |
| ATATCTGCCGCATCCTAGAA+AGG | - | chr4.1:20809214-20809233 | None:intergenic | 45.0% | |
| CACCTGCTAAAGACTCCAAA+GGG | + | chr4.1:20809285-20809304 | MS.gene31950:CDS | 45.0% | |
| CCAAAGGATGCTGCCAATAA+CGG | + | chr4.1:20809395-20809414 | MS.gene31950:CDS | 45.0% | |
| GCATCCTTTGGTTGTGATTC+AGG | - | chr4.1:20809386-20809405 | None:intergenic | 45.0% | |
| GGAAGCTGAGGATTACAAAG+TGG | + | chr4.1:20808849-20808868 | MS.gene31950:CDS | 45.0% | |
| GGATCAGCACCTAAACCAAA+TGG | + | chr4.1:20809341-20809360 | MS.gene31950:CDS | 45.0% | |
| TCTCAATGTTGGCAACCTCA+CGG | - | chr4.1:20809112-20809131 | None:intergenic | 45.0% | |
| TGCACCTGAATCACAACCAA+AGG | + | chr4.1:20809379-20809398 | MS.gene31950:CDS | 45.0% | |
| TGCTAAAGACTCCAAAGGGA+AGG | + | chr4.1:20809289-20809308 | MS.gene31950:CDS | 45.0% | |
| TGTGATTCAGGTGCAACTTC+CGG | - | chr4.1:20809374-20809393 | None:intergenic | 45.0% | |
| ! | TTCTTTTCCGTCCTTCCCTT+TGG | - | chr4.1:20809303-20809322 | None:intergenic | 45.0% |
| !! | GGTGCTGATCCATTTGGTTT+AGG | - | chr4.1:20809332-20809351 | None:intergenic | 45.0% |
| !! | GGTTTAGGTGCTGATCCATT+TGG | - | chr4.1:20809338-20809357 | None:intergenic | 45.0% |
| !! | TTCCCTTTGGAGTCTTTAGC+AGG | - | chr4.1:20809290-20809309 | None:intergenic | 45.0% |
| CCATCAGTTACTGTCCTCCA+AGG | + | chr4.1:20809164-20809183 | MS.gene31950:CDS | 50.0% | |
| CCGTTATTGGCAGCATCCTT+TGG | - | chr4.1:20809398-20809417 | None:intergenic | 50.0% | |
| CCTTGGAGGACAGTAACTGA+TGG | - | chr4.1:20809167-20809186 | None:intergenic | 50.0% | |
| CTCAATGTTGGCAACCTCAC+GGG | - | chr4.1:20809111-20809130 | None:intergenic | 50.0% | |
| GCACCTGCTAAAGACTCCAA+AGG | + | chr4.1:20809284-20809303 | MS.gene31950:CDS | 50.0% | |
| GGAGGAATCATGTGAAGTGG+TGG | - | chr4.1:20809257-20809276 | None:intergenic | 50.0% | |
| GGAGGAGGAATCATGTGAAG+TGG | - | chr4.1:20809260-20809279 | None:intergenic | 50.0% | |
| TCAATGTTGGCAACCTCACG+GGG | - | chr4.1:20809110-20809129 | None:intergenic | 50.0% | |
| TTGGAGTCTTTAGCAGGTGC+AGG | - | chr4.1:20809284-20809303 | None:intergenic | 50.0% | |
| !! | ACTTCCGGTGCTGATCCATT+TGG | - | chr4.1:20809359-20809378 | None:intergenic | 50.0% |
| CGCATCCTAGAAAGGTCGGT+GGG | - | chr4.1:20809206-20809225 | None:intergenic | 55.0% | |
| CTGCCGCATCCTAGAAAGGT+CGG | - | chr4.1:20809210-20809229 | None:intergenic | 55.0% | |
| GAAAGAAGCTCCTGCCACTG+AGG | + | chr4.1:20809436-20809455 | MS.gene31950:CDS | 55.0% | |
| GAGTCTTTAGCAGGTGCAGG+TGG | - | chr4.1:20809281-20809300 | None:intergenic | 55.0% | |
| GCAAACCCACCGACCTTTCT+AGG | + | chr4.1:20809198-20809217 | MS.gene31950:CDS | 55.0% | |
| GTGGCAGGAGCTTCTTTCTG+AGG | - | chr4.1:20809434-20809453 | None:intergenic | 55.0% | |
| TCTTTAGCAGGTGCAGGTGG+AGG | - | chr4.1:20809278-20809297 | None:intergenic | 55.0% | |
| TGGGGAGATAATTCCCCGTG+AGG | + | chr4.1:20809094-20809113 | MS.gene31950:CDS | 55.0% | |
| !! | CCACCGACCTTTCTAGGATG+CGG | + | chr4.1:20809204-20809223 | MS.gene31950:CDS | 55.0% |
| CCGCATCCTAGAAAGGTCGG+TGG | - | chr4.1:20809207-20809226 | None:intergenic | 60.0% | |
| TGCAGCAGACTGCTCCTCAG+TGG | - | chr4.1:20809453-20809472 | None:intergenic | 60.0% | |
| TTAGCAGGTGCAGGTGGAGG+AGG | - | chr4.1:20809275-20809294 | None:intergenic | 60.0% | |
| ! | CGGTGGGTTTGCCTGGCTTT+GGG | - | chr4.1:20809190-20809209 | None:intergenic | 60.0% |
| ! | GAAAGGTCGGTGGGTTTGCC+TGG | - | chr4.1:20809197-20809216 | None:intergenic | 60.0% |
| ! | TCGGTGGGTTTGCCTGGCTT+TGG | - | chr4.1:20809191-20809210 | None:intergenic | 60.0% |
| !! | GTTTGCCTGGCTTTGGGCCT+TGG | - | chr4.1:20809184-20809203 | None:intergenic | 60.0% |
| GCAGACTGCTCCTCAGTGGC+AGG | - | chr4.1:20809449-20809468 | None:intergenic | 65.0% | |
| GTCCTCCAAGGCCCAAAGCC+AGG | + | chr4.1:20809176-20809195 | MS.gene31950:CDS | 65.0% | |
| !! | TGCCTGGCTTTGGGCCTTGG+AGG | - | chr4.1:20809181-20809200 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 20808829 | 20809475 | 20808829 | ID=MS.gene31950 |
| chr4.1 | mRNA | 20808829 | 20809475 | 20808829 | ID=MS.gene31950.t1;Parent=MS.gene31950 |
| chr4.1 | exon | 20808829 | 20808936 | 20808829 | ID=MS.gene31950.t1.exon1;Parent=MS.gene31950.t1 |
| chr4.1 | CDS | 20808829 | 20808936 | 20808829 | ID=cds.MS.gene31950.t1;Parent=MS.gene31950.t1 |
| chr4.1 | exon | 20809032 | 20809475 | 20809032 | ID=MS.gene31950.t1.exon2;Parent=MS.gene31950.t1 |
| chr4.1 | CDS | 20809032 | 20809475 | 20809032 | ID=cds.MS.gene31950.t1;Parent=MS.gene31950.t1 |
| Gene Sequence |
| Protein sequence |
