Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32510.t1 | XP_024641057.1 | 74.2 | 256 | 54 | 4 | 1 | 245 | 1 | 255 | 2.70E-88 | 335.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32510.t1 | Q8W487 | 40.0 | 190 | 106 | 4 | 63 | 244 | 60 | 249 | 3.5e-28 | 126.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32510.t1 | A0A396I021 | 74.2 | 256 | 54 | 4 | 1 | 245 | 1 | 255 | 2.0e-88 | 335.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene32510 | MS.gene46105 | PPI |
| MS.gene32510 | MS.gene46104 | PPI |
| MS.gene000604 | MS.gene32510 | PPI |
| MS.gene32510 | MS.gene89319 | PPI |
| MS.gene32510 | MS.gene055201 | PPI |
| MS.gene32510 | MS.gene040271 | PPI |
| MS.gene32510 | MS.gene040272 | PPI |
| MS.gene32510 | MS.gene67544 | PPI |
| MS.gene32510 | MS.gene034255 | PPI |
| MS.gene32510 | MS.gene053230 | PPI |
| MS.gene32510 | MS.gene040269 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32510.t1 | MTR_5g080880 | 75.686 | 255 | 50 | 4 | 1 | 244 | 1 | 254 | 3.05e-124 | 356 |
| MS.gene32510.t1 | MTR_5g080870 | 66.935 | 248 | 51 | 5 | 1 | 245 | 1 | 220 | 1.34e-101 | 299 |
| MS.gene32510.t1 | MTR_7g063520 | 38.956 | 249 | 125 | 7 | 8 | 244 | 20 | 253 | 2.81e-46 | 155 |
| MS.gene32510.t1 | MTR_4g006560 | 33.793 | 145 | 92 | 2 | 104 | 244 | 91 | 235 | 7.91e-20 | 85.5 |
| MS.gene32510.t1 | MTR_1g013120 | 28.270 | 237 | 146 | 10 | 24 | 244 | 4 | 232 | 1.37e-14 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32510.t1 | AT3G55605 | 40.590 | 271 | 120 | 10 | 1 | 244 | 1 | 257 | 1.23e-44 | 150 |
| MS.gene32510.t1 | AT5G05990 | 36.364 | 264 | 136 | 7 | 5 | 244 | 3 | 258 | 1.83e-42 | 145 |
| MS.gene32510.t1 | AT2G39795 | 37.302 | 252 | 136 | 8 | 6 | 244 | 7 | 249 | 4.77e-41 | 141 |
| MS.gene32510.t1 | AT5G02050 | 40.758 | 211 | 100 | 6 | 55 | 244 | 60 | 266 | 4.19e-38 | 134 |
| MS.gene32510.t1 | AT2G39790 | 31.984 | 247 | 159 | 6 | 1 | 245 | 1 | 240 | 3.20e-27 | 105 |
| MS.gene32510.t1 | AT1G15870 | 29.861 | 144 | 98 | 3 | 104 | 244 | 97 | 240 | 3.11e-14 | 70.5 |
| MS.gene32510.t1 | AT1G80720 | 29.747 | 158 | 106 | 5 | 92 | 244 | 31 | 188 | 5.92e-12 | 63.2 |
| MS.gene32510.t1 | AT1G80720 | 29.747 | 158 | 106 | 5 | 92 | 244 | 83 | 240 | 1.08e-11 | 63.2 |
Find 44 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATCATCAACTATCTTAAA+TGG | 0.152055 | 5.1:-62751081 | None:intergenic |
| TTATCCGCAGCTTGTTTATT+AGG | 0.223286 | 5.1:+62751307 | MS.gene32510:CDS |
| ATCAGTTCATAAGACTTAAA+AGG | 0.242549 | 5.1:+62751106 | MS.gene32510:CDS |
| AAGCACAACAAATTTCTTAC+TGG | 0.307443 | 5.1:+62751798 | MS.gene32510:CDS |
| TAAGAAGGATGGGACCTCTT+TGG | 0.326135 | 5.1:+62751251 | MS.gene32510:CDS |
| CGAAGTCAAGCACTTTCGTT+AGG | 0.372011 | 5.1:+62750594 | MS.gene32510:CDS |
| CAAGCATTCCACTTTCAGTC+AGG | 0.380172 | 5.1:+62751223 | MS.gene32510:CDS |
| ATTACTTGGTGAGGCTGAAT+AGG | 0.381828 | 5.1:+62751848 | MS.gene32510:CDS |
| CTGCGGATAACCATCTCATC+AGG | 0.394847 | 5.1:-62751294 | None:intergenic |
| CTCTTATTTCTAAGTACTTG+TGG | 0.408675 | 5.1:-62751765 | None:intergenic |
| TTCAGTCAGGGTTTCTAAGA+AGG | 0.411975 | 5.1:+62751236 | MS.gene32510:CDS |
| AACAGATTTGTTATTATGAC+TGG | 0.418204 | 5.1:+62751340 | MS.gene32510:CDS |
| AGTAAGAAATTTGTTGTGCT+TGG | 0.424406 | 5.1:-62751796 | None:intergenic |
| CAAGAAAAGTAGAGATTACT+TGG | 0.433788 | 5.1:+62751834 | MS.gene32510:CDS |
| CACAAGTACTTAGAAATAAG+AGG | 0.434165 | 5.1:+62751766 | MS.gene32510:CDS |
| GATCATCAACTATCTTAAAT+GGG | 0.461342 | 5.1:-62751080 | None:intergenic |
| TTAGAAACCCTGACTGAAAG+TGG | 0.461666 | 5.1:-62751231 | None:intergenic |
| CTCGTAGCGGTTGACGGCAG+TGG | 0.471104 | 5.1:-62750678 | None:intergenic |
| AAGAAAAGTCTCATCGGAGT+TGG | 0.473820 | 5.1:-62750753 | None:intergenic |
| GAACATGATAATCATCAAAC+GGG | 0.483902 | 5.1:+62751189 | MS.gene32510:CDS |
| AGAACATGATAATCATCAAA+CGG | 0.497010 | 5.1:+62751188 | MS.gene32510:CDS |
| AGAAAAGTCTCATCGGAGTT+GGG | 0.497268 | 5.1:-62750752 | None:intergenic |
| GTCAGGGTTTCTAAGAAGGA+TGG | 0.501378 | 5.1:+62751240 | MS.gene32510:CDS |
| TGAACCCTCGTAGCGGTTGA+CGG | 0.507166 | 5.1:-62750684 | None:intergenic |
| AGACTTAAAAGGACTTATCA+AGG | 0.514679 | 5.1:+62751117 | MS.gene32510:CDS |
| ATCTTAAATGGGAAATTGCT+CGG | 0.515748 | 5.1:-62751069 | None:intergenic |
| GTTTACAGCGCGGAACTCAT+CGG | 0.541419 | 5.1:-62750813 | None:intergenic |
| AGGTGAAGAAATTCATGTTG+AGG | 0.546869 | 5.1:+62751137 | MS.gene32510:CDS |
| AAGCATTCCACTTTCAGTCA+GGG | 0.546965 | 5.1:+62751224 | MS.gene32510:CDS |
| GAAGTCAAGCACTTTCGTTA+GGG | 0.555276 | 5.1:+62750595 | MS.gene32510:CDS |
| TCAGGGTTTCTAAGAAGGAT+GGG | 0.578315 | 5.1:+62751241 | MS.gene32510:CDS |
| TGTTGCTTACCCTGATGAGA+TGG | 0.579238 | 5.1:+62751284 | MS.gene32510:CDS |
| CACTGCCGTCAACCGCTACG+AGG | 0.581926 | 5.1:+62750679 | MS.gene32510:CDS |
| AAGTGGAATGCTTGATTGAG+TGG | 0.598328 | 5.1:-62751214 | None:intergenic |
| TAAACGAAGAAAAGTCTCAT+CGG | 0.611707 | 5.1:-62750759 | None:intergenic |
| AAAGTAGAGATTACTTGGTG+AGG | 0.619484 | 5.1:+62751839 | MS.gene32510:CDS |
| GTTGACGGCAGTGGAAAGCA+CGG | 0.620720 | 5.1:-62750669 | None:intergenic |
| AACACAATTGAACTCCAAAG+AGG | 0.630953 | 5.1:-62751265 | None:intergenic |
| ACTGCCGTCAACCGCTACGA+GGG | 0.639281 | 5.1:+62750680 | MS.gene32510:CDS |
| GATTCCTAATAAACAAGCTG+CGG | 0.642181 | 5.1:-62751311 | None:intergenic |
| TCAGAAGAAAAGTGAAACGT+AGG | 0.644962 | 5.1:-62750713 | None:intergenic |
| AAGTGCTTGACTTCGAACGA+AGG | 0.657096 | 5.1:-62750586 | None:intergenic |
| TGCGGATAACCATCTCATCA+GGG | 0.665501 | 5.1:-62751293 | None:intergenic |
| GAACAAATGAACCCTCGTAG+CGG | 0.717050 | 5.1:-62750691 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCATGAGTTTAATAAATTA+AGG | - | chr5.1:62750847-62750866 | None:intergenic | 15.0% |
| !!! | AAATGTTGAAAATTTGATTT+TGG | + | chr5.1:62751025-62751044 | MS.gene32510:intron | 15.0% |
| !! | AATTGAAAGTAATACAAACA+AGG | - | chr5.1:62751664-62751683 | None:intergenic | 20.0% |
| !! | ATGGTATGAAAATTTGAATT+TGG | + | chr5.1:62750970-62750989 | MS.gene32510:intron | 20.0% |
| !! | TTAATAAATTAAGGAAGACA+AGG | - | chr5.1:62750838-62750857 | None:intergenic | 20.0% |
| !!! | AATTTGATTTTGGTTGATAT+AGG | + | chr5.1:62751035-62751054 | MS.gene32510:intron | 20.0% |
| !!! | ACATCAGTGATTTTAAATAA+AGG | + | chr5.1:62751443-62751462 | MS.gene32510:intron | 20.0% |
| !!! | ATTTGATTTTGGTTGATATA+GGG | + | chr5.1:62751036-62751055 | MS.gene32510:intron | 20.0% |
| !!! | ATTTTAAATAAAGGCTATAG+CGG | + | chr5.1:62751452-62751471 | MS.gene32510:intron | 20.0% |
| ! | AGAACATGATAATCATCAAA+CGG | + | chr5.1:62751188-62751207 | MS.gene32510:CDS | 25.0% |
| ! | AGATCATCAACTATCTTAAA+TGG | - | chr5.1:62751084-62751103 | None:intergenic | 25.0% |
| ! | ATCAGTTCATAAGACTTAAA+AGG | + | chr5.1:62751106-62751125 | MS.gene32510:CDS | 25.0% |
| ! | GATCATCAACTATCTTAAAT+GGG | - | chr5.1:62751083-62751102 | None:intergenic | 25.0% |
| !! | ATTTTCATACCATTACGAAA+TGG | - | chr5.1:62750963-62750982 | None:intergenic | 25.0% |
| !! | TTTTCATACCATTACGAAAT+GGG | - | chr5.1:62750962-62750981 | None:intergenic | 25.0% |
| !!! | AACAGATTTGTTATTATGAC+TGG | + | chr5.1:62751340-62751359 | MS.gene32510:CDS | 25.0% |
| AAAAAAGACCCATTTCGTAA+TGG | + | chr5.1:62750951-62750970 | MS.gene32510:intron | 30.0% | |
| AAGCACAACAAATTTCTTAC+TGG | + | chr5.1:62751798-62751817 | MS.gene32510:CDS | 30.0% | |
| AATCAGAATGAAGTACATAC+TGG | - | chr5.1:62751374-62751393 | None:intergenic | 30.0% | |
| AGACTTAAAAGGACTTATCA+AGG | + | chr5.1:62751117-62751136 | MS.gene32510:CDS | 30.0% | |
| ATCTTAAATGGGAAATTGCT+CGG | - | chr5.1:62751072-62751091 | None:intergenic | 30.0% | |
| ATTGATCAGAAAAACTCAAC+TGG | + | chr5.1:62750637-62750656 | MS.gene32510:CDS | 30.0% | |
| CAAGAAAAGTAGAGATTACT+TGG | + | chr5.1:62751834-62751853 | MS.gene32510:CDS | 30.0% | |
| CACAAGTACTTAGAAATAAG+AGG | + | chr5.1:62751766-62751785 | MS.gene32510:CDS | 30.0% | |
| GAACATGATAATCATCAAAC+GGG | + | chr5.1:62751189-62751208 | MS.gene32510:CDS | 30.0% | |
| TAAACGAAGAAAAGTCTCAT+CGG | - | chr5.1:62750762-62750781 | None:intergenic | 30.0% | |
| TGAACTTATACCTTATAAGC+TGG | + | chr5.1:62750921-62750940 | MS.gene32510:intron | 30.0% | |
| TTGATCAGAAAAACTCAACT+GGG | + | chr5.1:62750638-62750657 | MS.gene32510:CDS | 30.0% | |
| TTTAAGCATACCAGCTTATA+AGG | - | chr5.1:62750934-62750953 | None:intergenic | 30.0% | |
| ! | GTGCATTCAATATTTGAAAC+CGG | + | chr5.1:62751416-62751435 | MS.gene32510:intron | 30.0% |
| ! | TGCATTCAATATTTGAAACC+GGG | + | chr5.1:62751417-62751436 | MS.gene32510:intron | 30.0% |
| !! | AGTAAGAAATTTGTTGTGCT+TGG | - | chr5.1:62751799-62751818 | None:intergenic | 30.0% |
| !! | CTCTTATTTCTAAGTACTTG+TGG | - | chr5.1:62751768-62751787 | None:intergenic | 30.0% |
| AACACAATTGAACTCCAAAG+AGG | - | chr5.1:62751268-62751287 | None:intergenic | 35.0% | |
| AGGTGAAGAAATTCATGTTG+AGG | + | chr5.1:62751137-62751156 | MS.gene32510:CDS | 35.0% | |
| ATCAAAAATCACTTGTGCTC+AGG | + | chr5.1:62750787-62750806 | MS.gene32510:CDS | 35.0% | |
| GATTCCTAATAAACAAGCTG+CGG | - | chr5.1:62751314-62751333 | None:intergenic | 35.0% | |
| TAAAATCACTGATGTAACCC+CGG | - | chr5.1:62751438-62751457 | None:intergenic | 35.0% | |
| TCAGAAGAAAAGTGAAACGT+AGG | - | chr5.1:62750716-62750735 | None:intergenic | 35.0% | |
| TCCAAATCCCTGGAAAAAAA+TGG | - | chr5.1:62751724-62751743 | None:intergenic | 35.0% | |
| ! | AAAGTAGAGATTACTTGGTG+AGG | + | chr5.1:62751839-62751858 | MS.gene32510:CDS | 35.0% |
| ! | AACTACTATTTTCCGCGATT+TGG | + | chr5.1:62751536-62751555 | MS.gene32510:intron | 35.0% |
| ! | GCATTCAATATTTGAAACCG+GGG | + | chr5.1:62751418-62751437 | MS.gene32510:intron | 35.0% |
| ! | TTATCCGCAGCTTGTTTATT+AGG | + | chr5.1:62751307-62751326 | MS.gene32510:CDS | 35.0% |
| !!! | AGATTCTGCCATTTTTTTCC+AGG | + | chr5.1:62751713-62751732 | MS.gene32510:intron | 35.0% |
| !!! | ATTTTGGTTGATATAGGGTG+AGG | + | chr5.1:62751041-62751060 | MS.gene32510:intron | 35.0% |
| !!! | GATTCTGCCATTTTTTTCCA+GGG | + | chr5.1:62751714-62751733 | MS.gene32510:intron | 35.0% |
| !!! | TGAGGTTTCGAGTTTTGATT+TGG | + | chr5.1:62751155-62751174 | MS.gene32510:CDS | 35.0% |
| !!! | TGCAGTTCAGATTGTTGTTT+TGG | + | chr5.1:62751624-62751643 | MS.gene32510:intron | 35.0% |
| AAGAAAAGTCTCATCGGAGT+TGG | - | chr5.1:62750756-62750775 | None:intergenic | 40.0% | |
| AAGCATTCCACTTTCAGTCA+GGG | + | chr5.1:62751224-62751243 | MS.gene32510:CDS | 40.0% | |
| AAGTGGAATGCTTGATTGAG+TGG | - | chr5.1:62751217-62751236 | None:intergenic | 40.0% | |
| AGAAAAGTCTCATCGGAGTT+GGG | - | chr5.1:62750755-62750774 | None:intergenic | 40.0% | |
| CCAATCTCAATTCCAAATCG+CGG | - | chr5.1:62751551-62751570 | None:intergenic | 40.0% | |
| CCACTATAGCTGCTATGATA+TGG | - | chr5.1:62751480-62751499 | None:intergenic | 40.0% | |
| CCATATCATAGCAGCTATAG+TGG | + | chr5.1:62751477-62751496 | MS.gene32510:intron | 40.0% | |
| GAAGTCAAGCACTTTCGTTA+GGG | + | chr5.1:62750595-62750614 | MS.gene32510:CDS | 40.0% | |
| TTAGAAACCCTGACTGAAAG+TGG | - | chr5.1:62751234-62751253 | None:intergenic | 40.0% | |
| ! | ATTACTTGGTGAGGCTGAAT+AGG | + | chr5.1:62751848-62751867 | MS.gene32510:CDS | 40.0% |
| ! | CGCGATTTGGAATTGAGATT+GGG | + | chr5.1:62751549-62751568 | MS.gene32510:intron | 40.0% |
| ! | GATTTGGAATTGAGATTGGG+TGG | + | chr5.1:62751552-62751571 | MS.gene32510:intron | 40.0% |
| ! | GCTATAGCACTTTTGCATAG+CGG | + | chr5.1:62751503-62751522 | MS.gene32510:intron | 40.0% |
| ! | TCAGGGTTTCTAAGAAGGAT+GGG | + | chr5.1:62751241-62751260 | MS.gene32510:CDS | 40.0% |
| ! | TTCAGTCAGGGTTTCTAAGA+AGG | + | chr5.1:62751236-62751255 | MS.gene32510:CDS | 40.0% |
| ! | TTTTTCCAGGGATTTGGATG+AGG | + | chr5.1:62751726-62751745 | MS.gene32510:intron | 40.0% |
| !!! | GCCATTTTTTTCCAGGGATT+TGG | + | chr5.1:62751720-62751739 | MS.gene32510:intron | 40.0% |
| AAGTGCTTGACTTCGAACGA+AGG | - | chr5.1:62750589-62750608 | None:intergenic | 45.0% | |
| CAAGCATTCCACTTTCAGTC+AGG | + | chr5.1:62751223-62751242 | MS.gene32510:CDS | 45.0% | |
| CGAAGTCAAGCACTTTCGTT+AGG | + | chr5.1:62750594-62750613 | MS.gene32510:CDS | 45.0% | |
| GAACAAATGAACCCTCGTAG+CGG | - | chr5.1:62750694-62750713 | None:intergenic | 45.0% | |
| GTCTCTACAACAAGCAGTAC+AGG | - | chr5.1:62751587-62751606 | None:intergenic | 45.0% | |
| TAAGAAGGATGGGACCTCTT+TGG | + | chr5.1:62751251-62751270 | MS.gene32510:CDS | 45.0% | |
| TGCGGATAACCATCTCATCA+GGG | - | chr5.1:62751296-62751315 | None:intergenic | 45.0% | |
| ! | CCGCGATTTGGAATTGAGAT+TGG | + | chr5.1:62751548-62751567 | MS.gene32510:intron | 45.0% |
| ! | GTCAGGGTTTCTAAGAAGGA+TGG | + | chr5.1:62751240-62751259 | MS.gene32510:CDS | 45.0% |
| ! | TGTTGCTTACCCTGATGAGA+TGG | + | chr5.1:62751284-62751303 | MS.gene32510:CDS | 45.0% |
| CTGCGGATAACCATCTCATC+AGG | - | chr5.1:62751297-62751316 | None:intergenic | 50.0% | |
| GGAAGACAAGGTTTACAGCG+CGG | - | chr5.1:62750826-62750845 | None:intergenic | 50.0% | |
| GTTTACAGCGCGGAACTCAT+CGG | - | chr5.1:62750816-62750835 | None:intergenic | 50.0% | |
| CAACGCCTCATCCAAATCCC+TGG | - | chr5.1:62751734-62751753 | None:intergenic | 55.0% | |
| GTTGACGGCAGTGGAAAGCA+CGG | - | chr5.1:62750672-62750691 | None:intergenic | 55.0% | |
| TGAACCCTCGTAGCGGTTGA+CGG | - | chr5.1:62750687-62750706 | None:intergenic | 55.0% | |
| ACTGCCGTCAACCGCTACGA+GGG | + | chr5.1:62750680-62750699 | MS.gene32510:CDS | 60.0% | |
| CACTGCCGTCAACCGCTACG+AGG | + | chr5.1:62750679-62750698 | MS.gene32510:CDS | 65.0% | |
| CTCGTAGCGGTTGACGGCAG+TGG | - | chr5.1:62750681-62750700 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 62750569 | 62751894 | 62750569 | ID=MS.gene32510 |
| chr5.1 | mRNA | 62750569 | 62751894 | 62750569 | ID=MS.gene32510.t1;Parent=MS.gene32510 |
| chr5.1 | exon | 62750569 | 62750829 | 62750569 | ID=MS.gene32510.t1.exon1;Parent=MS.gene32510.t1 |
| chr5.1 | CDS | 62750569 | 62750829 | 62750569 | ID=cds.MS.gene32510.t1;Parent=MS.gene32510.t1 |
| chr5.1 | exon | 62751057 | 62751373 | 62751057 | ID=MS.gene32510.t1.exon2;Parent=MS.gene32510.t1 |
| chr5.1 | CDS | 62751057 | 62751373 | 62751057 | ID=cds.MS.gene32510.t1;Parent=MS.gene32510.t1 |
| chr5.1 | exon | 62751735 | 62751894 | 62751735 | ID=MS.gene32510.t1.exon3;Parent=MS.gene32510.t1 |
| chr5.1 | CDS | 62751735 | 62751894 | 62751735 | ID=cds.MS.gene32510.t1;Parent=MS.gene32510.t1 |
| Gene Sequence |
| Protein sequence |
