AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32646

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32646.t1 MTR_3g106900 99.064 641 6 0 1 641 1 641 0.0 1313
MS.gene32646.t1 MTR_4g114960 57.231 650 220 11 1 641 1 601 0.0 746
MS.gene32646.t1 MTR_2g008170 45.963 644 275 11 1 618 1 597 3.44e-169 496
MS.gene32646.t1 MTR_8g008860 44.038 629 289 12 1 618 1 577 7.22e-166 487
MS.gene32646.t1 MTR_3g080490 43.503 354 151 5 4 357 3 307 2.86e-95 313
MS.gene32646.t1 MTR_2g037980 34.540 359 178 8 2 355 3 309 1.35e-59 210
MS.gene32646.t1 MTR_4g127440 30.169 590 316 13 2 577 3 510 5.40e-59 208
MS.gene32646.t1 MTR_4g115420 46.053 152 79 2 2 151 5 155 5.74e-38 148
MS.gene32646.t1 MTR_4g115420 28.493 365 199 9 227 577 172 488 7.56e-28 118
MS.gene32646.t1 MTR_8g106040 26.629 353 203 6 6 353 5 306 3.24e-33 133
MS.gene32646.t1 MTR_0530s0020 26.606 327 178 6 32 355 17 284 3.54e-30 125
MS.gene32646.t1 MTR_6g059380 34.437 151 95 1 4 150 7 157 6.14e-19 90.9
MS.gene32646.t1 MTR_1g033880 35.570 149 82 4 7 149 8 148 2.98e-17 84.7
MS.gene32646.t1 MTR_8g089850 30.220 182 117 5 1 178 1 176 8.03e-16 79.3
MS.gene32646.t1 MTR_3g086670 33.333 147 93 3 6 150 5 148 1.39e-15 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32646.t1 AT2G25430 74.887 661 124 15 5 638 4 649 0.0 898
MS.gene32646.t1 AT4G32285 72.430 642 155 10 5 638 4 631 0.0 867
MS.gene32646.t1 AT4G32285 72.430 642 155 10 5 638 4 631 0.0 867
MS.gene32646.t1 AT1G03050 45.609 649 255 14 1 610 1 590 0.0 533
MS.gene32646.t1 AT4G02650 44.665 656 281 15 1 626 1 604 4.92e-164 483
MS.gene32646.t1 AT1G05020 40.199 403 180 6 4 400 3 350 5.81e-96 308
MS.gene32646.t1 AT4G25940 31.954 604 300 18 2 572 3 528 2.20e-72 245
MS.gene32646.t1 AT1G14910 33.645 535 262 18 2 514 3 466 1.43e-70 242
MS.gene32646.t1 AT1G14910 33.271 535 259 19 2 514 3 461 7.72e-67 232
MS.gene32646.t1 AT2G01600 30.146 617 309 19 2 594 3 521 7.01e-65 224
MS.gene32646.t1 AT5G57200 34.722 360 175 8 2 355 3 308 8.77e-62 216
MS.gene32646.t1 AT5G35200 43.333 150 82 2 6 153 11 159 1.80e-34 138
MS.gene32646.t1 AT5G35200 28.212 358 202 11 227 575 174 485 7.39e-24 106
MS.gene32646.t1 AT5G35200 43.333 150 82 2 6 153 11 159 1.80e-34 138
MS.gene32646.t1 AT5G35200 28.212 358 202 11 227 575 174 485 7.39e-24 106
MS.gene32646.t1 AT1G68110 26.836 354 186 11 7 353 5 292 1.88e-18 88.2
MS.gene32646.t1 AT1G33340 35.099 151 92 2 3 150 6 153 1.16e-17 85.9
MS.gene32646.t1 AT4G40080 34.014 147 91 4 10 150 11 157 1.08e-15 79.7
MS.gene32646.t1 AT1G25240 35.811 148 81 5 7 148 5 144 1.86e-15 79.0
MS.gene32646.t1 AT1G25240 35.811 148 81 5 7 148 6 145 1.93e-15 79.0

Find 213 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTCCTCTCTGGACTCTT+TGG 0.225582 3.1:-84680561 None:intergenic
TGCTAGCAGTGTGGCGTTGC+CGG 0.225948 3.1:+84681061 MS.gene32646:CDS
CTAGTAATTCTTTGAACTTC+CGG 0.266921 3.1:-84680489 None:intergenic
TCAGGCTCTGGTGGAGGTGG+CGG 0.270903 3.1:-84680672 None:intergenic
TTCTTGGCTTGTAGGCCAAC+TGG 0.276217 3.1:+84680225 MS.gene32646:CDS
GGAGGTCCCATTCCATGAAC+TGG 0.286179 3.1:-84681359 None:intergenic
GAGTCATTTGCAGAGGCTTT+TGG 0.294432 3.1:+84680197 MS.gene32646:CDS
TGGATCGTTTCTTGGCTTGT+AGG 0.296888 3.1:+84680217 MS.gene32646:CDS
TGCACAGATCCATCTGGGGC+TGG 0.298625 3.1:-84681119 None:intergenic
ATACATGCCATTCAACAATA+AGG 0.303811 3.1:-84680985 None:intergenic
ATGAGGCTCATTCAAGTTCT+TGG 0.306921 3.1:+84679860 MS.gene32646:CDS
CAGGCTCTGGTGGAGGTGGC+GGG 0.310633 3.1:-84680671 None:intergenic
GTGGTGGAGGTGGGGATGAG+AGG 0.323272 3.1:+84679977 MS.gene32646:CDS
TCCTCCGTAAACTGCGGTTT+TGG 0.326695 3.1:-84680702 None:intergenic
AAGAATTACTAGTAAGTTGT+TGG 0.329377 3.1:+84680500 MS.gene32646:CDS
CAAACCCCTGCAGCTGAACC+TGG 0.330516 3.1:+84680879 MS.gene32646:CDS
TTTGCGTTGGCACTCTTTGC+TGG 0.331820 3.1:+84680777 MS.gene32646:CDS
TCTAGGCAGAAGAATGCTTT+AGG 0.335417 3.1:+84680948 MS.gene32646:CDS
ACAGGTGTTGTGGTCTTTCC+CGG 0.343337 3.1:-84681086 None:intergenic
GGTAGTGTTGGTGGTGGTGG+TGG 0.343928 3.1:+84679961 MS.gene32646:CDS
TGCCAAGCTGAAGTTACTTC+CGG 0.346193 3.1:-84680858 None:intergenic
GTGGGAGAGAAAATAATTTC+AGG 0.350857 3.1:+84680004 MS.gene32646:CDS
ACAAGAGGAGGAGCCGGTTC+AGG 0.352668 3.1:+84680605 MS.gene32646:CDS
TGCTGATATATGCGAGGTTT+TGG 0.359618 3.1:+84680317 MS.gene32646:CDS
TCAGGCTCAGGCTCTGGTGG+AGG 0.361839 3.1:-84680678 None:intergenic
TTGTTGCTTCTTCTCCATTT+CGG 0.363887 3.1:-84681207 None:intergenic
CAGTGTGGCGTTGCCGGGAC+CGG 0.367032 3.1:+84681067 MS.gene32646:CDS
GGAGCTTCCTCTTTCCTCTC+TGG 0.367047 3.1:-84680570 None:intergenic
GGCGAGATCCTCCGAGTATC+CGG 0.368486 3.1:+84680470 MS.gene32646:CDS
GCTGGTAGTGTTGGTGGTGG+TGG 0.373659 3.1:+84679958 MS.gene32646:CDS
TGCAGTCACTGCAGACGATC+AGG 0.373792 3.1:+84680746 MS.gene32646:CDS
TGTCGGGGAATGATGATAGA+AGG 0.374281 3.1:+84680118 MS.gene32646:CDS
GGCTCAGGCTCTGGTGGAGG+TGG 0.378487 3.1:-84680675 None:intergenic
GAGCAACATGGCGCCGGAAT+TGG 0.378632 3.1:+84679558 MS.gene32646:CDS
ATCTGTGCAGCCAGTTAATC+AGG 0.381955 3.1:+84681133 MS.gene32646:CDS
TGCATTGTATCCGTTGGTTA+AGG 0.382273 3.1:+84680278 MS.gene32646:CDS
TCAAAGGCCTTCACACAATC+AGG 0.395986 3.1:-84680375 None:intergenic
TTTGGCTCAGGCTCAGGCTC+TGG 0.396279 3.1:-84680684 None:intergenic
GCTTTCCCAGGTTCAGCTGC+AGG 0.404670 3.1:-84680885 None:intergenic
ATAATCTCTTCCTGGAAAAT+CGG 0.405006 3.1:-84679787 None:intergenic
TCAGCTGTGTCCAAGCGATT+GGG 0.406593 3.1:+84679694 MS.gene32646:CDS
CTTCTGCCTAGATAAATTGC+TGG 0.406648 3.1:-84680937 None:intergenic
CTGGGTTGGCTAAGAACAAT+AGG 0.407625 3.1:+84680244 MS.gene32646:CDS
TAGCATACATAATCTCTTCC+TGG 0.407692 3.1:-84679795 None:intergenic
ACTTCCGGCTGTCCATTTGA+TGG 0.408563 3.1:-84680843 None:intergenic
TATGGAGGTGGACAATTTAG+AGG 0.412015 3.1:+84680048 MS.gene32646:CDS
CTTTCCCAGGTTCAGCTGCA+GGG 0.412658 3.1:-84680884 None:intergenic
CTTGATCCCTTATTGTTGAA+TGG 0.413257 3.1:+84680978 MS.gene32646:CDS
GTTGTGGTCTTTCCCGGTCC+CGG 0.413441 3.1:-84681080 None:intergenic
GCGAACGGATCCTGATTAAC+TGG 0.416129 3.1:-84681143 None:intergenic
CTATGTGCAAATGGCCGAAA+TGG 0.416745 3.1:+84681193 MS.gene32646:CDS
CCGGCTAATAATAATGCAAA+TGG 0.417868 3.1:+84680804 MS.gene32646:CDS
TGGGAAGCCTTTCCATCAAA+TGG 0.420108 3.1:+84680831 MS.gene32646:CDS
GATTGGGGAAGACGCGTGAT+TGG 0.420367 3.1:+84679710 MS.gene32646:CDS
TTCAGCTGTGTCCAAGCGAT+TGG 0.424563 3.1:+84679693 MS.gene32646:CDS
ACAATCGCCACCTCCAATTC+CGG 0.424606 3.1:-84679571 None:intergenic
GCACAGATCCATCTGGGGCT+GGG 0.426777 3.1:-84681118 None:intergenic
TCCTCTTGTACTTCCAGCTT+AGG 0.430283 3.1:-84680591 None:intergenic
TGAGCCAAAACCGCAGTTTA+CGG 0.430872 3.1:+84680698 MS.gene32646:CDS
CCACCGAATGAGTATGAGTA+TGG 0.432260 3.1:+84680030 MS.gene32646:CDS
TGAACCTGGGAAAGCGGATT+GGG 0.433469 3.1:+84680893 MS.gene32646:CDS
ATCTCGTTCATATCCTGAAC+CGG 0.433714 3.1:-84680618 None:intergenic
ACTGGCTGCACAGATCCATC+TGG 0.435637 3.1:-84681125 None:intergenic
TTGTTACTGTGACTCCTTTG+AGG 0.437358 3.1:+84680142 MS.gene32646:CDS
AACAAAATGAACAATGGTAC+TGG 0.437938 3.1:+84681299 MS.gene32646:CDS
GCTAGCAGTGTGGCGTTGCC+GGG 0.438220 3.1:+84681062 MS.gene32646:CDS
CGGCTGTCCATTTGATGGAA+AGG 0.439223 3.1:-84680838 None:intergenic
TGAGGCTCATTCAAGTTCTT+GGG 0.442141 3.1:+84679861 MS.gene32646:CDS
AGCGGATTGGGAACTGGCAT+TGG 0.444215 3.1:+84680905 MS.gene32646:CDS
TACAATTGGTGTAAAGAAAG+TGG 0.445885 3.1:+84680444 MS.gene32646:CDS
GGCTCAGGCTCAGGCTCTGG+TGG 0.448716 3.1:-84680681 None:intergenic
ACCTCCCTCCTATGTGCAAA+TGG 0.449011 3.1:+84681184 MS.gene32646:CDS
TGGAGGAGTTTGTGAGGGAT+AGG 0.450640 3.1:+84680529 MS.gene32646:CDS
GGCACTCTTTGCTGGTGCAC+CGG 0.450777 3.1:+84680785 MS.gene32646:CDS
TACATGCCATTCAACAATAA+GGG 0.450802 3.1:-84680984 None:intergenic
AATGGTACTGGATACTATGC+TGG 0.457007 3.1:+84681311 MS.gene32646:CDS
TGGGAAAGCGGATTGGGAAC+TGG 0.460427 3.1:+84680899 MS.gene32646:CDS
AGACAGCCAGCAATTTATCT+AGG 0.466153 3.1:+84680931 MS.gene32646:CDS
GTGAGCACTTCCCAACTTAG+TGG 0.466638 3.1:+84681032 MS.gene32646:CDS
GGCTTGTAGGCCAACTGGGT+TGG 0.467428 3.1:+84680230 MS.gene32646:CDS
AGCACTCCCTCCACTAAGTT+GGG 0.468854 3.1:-84681042 None:intergenic
CTGAACCTGGGAAAGCGGAT+TGG 0.473866 3.1:+84680892 MS.gene32646:CDS
TCTTGGCTTGTAGGCCAACT+GGG 0.474549 3.1:+84680226 MS.gene32646:CDS
GACGAGTCACGACGAAGATC+CGG 0.474753 3.1:+84679600 MS.gene32646:CDS
TTAGAGGGGAAGGTGGGATG+AGG 0.476333 3.1:+84680064 MS.gene32646:CDS
GTGGGGATGAGAGGTTCAGT+GGG 0.476909 3.1:+84679986 MS.gene32646:CDS
TAGCACTCCCTCCACTAAGT+TGG 0.477701 3.1:-84681043 None:intergenic
CCATTTGCATTATTATTAGC+CGG 0.478356 3.1:-84680804 None:intergenic
CAGAAGAATGCTTTAGGTGG+TGG 0.478692 3.1:+84680954 MS.gene32646:CDS
TGTTACTGTGACTCCTTTGA+GGG 0.482095 3.1:+84680143 MS.gene32646:CDS
GACAATTTAGAGGGGAAGGT+GGG 0.482887 3.1:+84680058 MS.gene32646:CDS
GAGGTCCCATTCCATGAACT+GGG 0.483754 3.1:-84681358 None:intergenic
CATTTCGGCCATTTGCACAT+AGG 0.483880 3.1:-84681192 None:intergenic
ACCGCAGTTTACGGAGGACT+TGG 0.485521 3.1:+84680707 MS.gene32646:CDS
TAGTAAGTTGTTGGAGACAC+TGG 0.489566 3.1:+84680509 MS.gene32646:CDS
GGAGTATGGTAATACCCGTT+TGG 0.490570 3.1:-84681380 None:intergenic
AAAAGCGATTGGAGCGGTGA+AGG 0.491311 3.1:+84679504 MS.gene32646:CDS
GTGAAGGATCAGACGAGTAT+TGG 0.495455 3.1:+84679520 MS.gene32646:CDS
AGTTCCCAATCCGCTTTCCC+AGG 0.496887 3.1:-84680897 None:intergenic
CAACATGGCGCCGGAATTGG+AGG 0.497407 3.1:+84679561 MS.gene32646:CDS
GGTGATGTGAATGAAGTGTC+GGG 0.503105 3.1:+84680102 MS.gene32646:CDS
GAAAGAGGAAGCTCCTAAGC+TGG 0.504819 3.1:+84680578 MS.gene32646:CDS
GGACAATTTAGAGGGGAAGG+TGG 0.509390 3.1:+84680057 MS.gene32646:CDS
ACGCGTCTTCCCCAATCGCT+TGG 0.510059 3.1:-84679704 None:intergenic
TCTTTGAACTTCCGGATACT+CGG 0.511861 3.1:-84680481 None:intergenic
CCTCCATACTCATACTCATT+CGG 0.512264 3.1:-84680033 None:intergenic
CTGGCTGCACAGATCCATCT+GGG 0.512279 3.1:-84681124 None:intergenic
AGCACAATCCGAAAAGCGAT+TGG 0.514162 3.1:+84679493 MS.gene32646:CDS
GGGGTATAATTCTCAGGTGC+AGG 0.514218 3.1:-84680651 None:intergenic
ATGGAATGGGACCTCCAAAC+GGG 0.516808 3.1:+84681366 MS.gene32646:CDS
TTAGCTCTCCCAGCCCCAGA+TGG 0.517956 3.1:+84681110 MS.gene32646:CDS
TTCGGCCATTTGCACATAGG+AGG 0.519643 3.1:-84681189 None:intergenic
AGTGTGGCGTTGCCGGGACC+GGG 0.520277 3.1:+84681068 MS.gene32646:CDS
ATGTTGTTTGATAGAAAAGC+TGG 0.520386 3.1:+84679940 MS.gene32646:CDS
TTGGTGTAAAGAAAGTGGTT+TGG 0.520726 3.1:+84680449 MS.gene32646:CDS
ATGTTCAATGATGCGGCGAA+CGG 0.523167 3.1:-84681158 None:intergenic
GGTGGCGGGGTATAATTCTC+AGG 0.525615 3.1:-84680657 None:intergenic
ATGCTACGAGGAGAGGGACT+AGG 0.526966 3.1:+84679812 MS.gene32646:CDS
ATCTTCCCGAAAATCCTCTC+CGG 0.528840 3.1:-84680174 None:intergenic
TCCTTTGAGGGATATGAAGC+CGG 0.529821 3.1:+84680155 MS.gene32646:CDS
GGAAGTACAAGAGGAGGAGC+CGG 0.530728 3.1:+84680599 MS.gene32646:CDS
AAACCCCTGCAGCTGAACCT+GGG 0.532015 3.1:+84680880 MS.gene32646:CDS
TCAGGATATGAACGAGATCA+AGG 0.532360 3.1:+84680623 MS.gene32646:CDS
GGAGGAGTTTGTGAGGGATA+GGG 0.532775 3.1:+84680530 MS.gene32646:CDS
AGCTGTGGCATCAGTATGCA+AGG 0.533057 3.1:+84681249 MS.gene32646:CDS
CGGAGGACTTGGTGAATCTG+AGG 0.533271 3.1:+84680718 MS.gene32646:CDS
CTTATATACTTCTCACTCGC+CGG 0.534004 3.1:-84679619 None:intergenic
AGAAGAATGCTTTAGGTGGT+GGG 0.534327 3.1:+84680955 MS.gene32646:CDS
TGCAGCTGAACCTGGGAAAG+CGG 0.538233 3.1:+84680887 MS.gene32646:CDS
AGGCTCTGGTGGAGGTGGCG+GGG 0.540099 3.1:-84680670 None:intergenic
AAAGAGTCCAGAGAGGAAAG+AGG 0.540156 3.1:+84680563 MS.gene32646:CDS
GATAGAAAAGCTGGTAGTGT+TGG 0.540496 3.1:+84679949 MS.gene32646:CDS
CATGGCGCCGGAATTGGAGG+TGG 0.543914 3.1:+84679564 MS.gene32646:CDS
GGGGCTGGGAGAGCTAAAAC+AGG 0.544685 3.1:-84681104 None:intergenic
TTTCCCAGGTTCAGCTGCAG+GGG 0.545857 3.1:-84680883 None:intergenic
GGTGGACAATTTAGAGGGGA+AGG 0.546935 3.1:+84680054 MS.gene32646:CDS
ATGGTACTGGATACTATGCT+GGG 0.551613 3.1:+84681312 MS.gene32646:CDS
ATGGAGGTGGACAATTTAGA+GGG 0.551974 3.1:+84680049 MS.gene32646:CDS
ATGAGGAAGACGAGGTCGTA+TGG 0.552855 3.1:+84680081 MS.gene32646:CDS
GTTCATCGACTGATGAATGA+TGG 0.553308 3.1:+84679760 MS.gene32646:CDS
TGGCATCAGTATGCAAGGGA+TGG 0.556853 3.1:+84681254 MS.gene32646:CDS
CTTTCCATCAAATGGACAGC+CGG 0.557840 3.1:+84680839 MS.gene32646:CDS
TCAGGGTAATAGATTTGCGT+TGG 0.558037 3.1:+84680764 MS.gene32646:CDS
TTGTGAGGGATAGGGCTAAG+AGG 0.559656 3.1:+84680538 MS.gene32646:CDS
TCCTAAGCTGGAAGTACAAG+AGG 0.562685 3.1:+84680590 MS.gene32646:CDS
CAGCTGTGTCCAAGCGATTG+GGG 0.563623 3.1:+84679695 MS.gene32646:CDS
GGTGGGGATGAGAGGTTCAG+TGG 0.563806 3.1:+84679985 MS.gene32646:CDS
CATGGAATGGGACCTCCAAA+CGG 0.565171 3.1:+84681365 MS.gene32646:CDS
TAATAATGCAAATGGATCGT+GGG 0.567598 3.1:+84680812 MS.gene32646:CDS
AGAAAAGCTGGTAGTGTTGG+TGG 0.569447 3.1:+84679952 MS.gene32646:CDS
AATGAGTATGAGTATGGAGG+TGG 0.576242 3.1:+84680036 MS.gene32646:CDS
AGCACTTGCATAAGCATCAA+AGG 0.577337 3.1:-84680391 None:intergenic
TCGGCCATTTGCACATAGGA+GGG 0.577744 3.1:-84681188 None:intergenic
AGTAGCGAGCAACATGGCGC+CGG 0.578249 3.1:+84679552 MS.gene32646:CDS
ATTTGCACATAGGAGGGAGG+TGG 0.579870 3.1:-84681182 None:intergenic
TTTGATTGCATTGTATCCGT+TGG 0.580416 3.1:+84680272 MS.gene32646:CDS
AGCCGGAAGTAACTTCAGCT+TGG 0.581334 3.1:+84680856 MS.gene32646:CDS
GTATGGTAATACCCGTTTGG+AGG 0.584432 3.1:-84681377 None:intergenic
TTATGTATGCTACGAGGAGA+GGG 0.585734 3.1:+84679806 MS.gene32646:CDS
TATGCAAGGGATGGGATGCA+AGG 0.585796 3.1:+84681263 MS.gene32646:CDS
ATAATAATGCAAATGGATCG+TGG 0.587702 3.1:+84680811 MS.gene32646:CDS
ATTATGTATGCTACGAGGAG+AGG 0.593045 3.1:+84679805 MS.gene32646:CDS
TTGTTCTTAGCCAACCCAGT+TGG 0.593548 3.1:-84680240 None:intergenic
TTGAATGGCATGTATGATCA+AGG 0.598862 3.1:+84680993 MS.gene32646:CDS
GGAAGATGAGTCATTTGCAG+AGG 0.598868 3.1:+84680190 MS.gene32646:CDS
GCTTGACAAATTCTTTGACA+TGG 0.599304 3.1:+84680347 MS.gene32646:CDS
ATGCTTTGTATCTCGATCAG+AGG 0.601861 3.1:+84679908 MS.gene32646:CDS
TCCGGCTTCATATCCCTCAA+AGG 0.602526 3.1:-84680156 None:intergenic
AACTTGATGTCTTACTCGCG+TGG 0.604269 3.1:+84679652 MS.gene32646:CDS
TGGTGATGTGAATGAAGTGT+CGG 0.612307 3.1:+84680101 MS.gene32646:CDS
GACACTGGAGGAGTTTGTGA+GGG 0.619044 3.1:+84680524 MS.gene32646:CDS
GCAGTCACTGCAGACGATCA+GGG 0.621548 3.1:+84680747 MS.gene32646:CDS
TAAGCTGGAAGTACAAGAGG+AGG 0.625162 3.1:+84680593 MS.gene32646:CDS
AAAGCTGGTAGTGTTGGTGG+TGG 0.625469 3.1:+84679955 MS.gene32646:CDS
TGGAGGTGGACAATTTAGAG+GGG 0.626675 3.1:+84680050 MS.gene32646:CDS
TTGTGCATGAGCAGCAGCTG+TGG 0.626683 3.1:+84681234 MS.gene32646:CDS
AGACACTGGAGGAGTTTGTG+AGG 0.628043 3.1:+84680523 MS.gene32646:CDS
GGTCCCATTCCATGAACTGG+GGG 0.628379 3.1:-84681356 None:intergenic
AGGTGGAATGTTCAATGATG+CGG 0.628537 3.1:-84681165 None:intergenic
AGCACTTCCCAACTTAGTGG+AGG 0.634431 3.1:+84681035 MS.gene32646:CDS
GCCATTTGCACATAGGAGGG+AGG 0.634464 3.1:-84681185 None:intergenic
TGGTACTGGATACTATGCTG+GGG 0.635659 3.1:+84681313 MS.gene32646:CDS
GGCATCAGTATGCAAGGGAT+GGG 0.637136 3.1:+84681255 MS.gene32646:CDS
ACCAAGTCCTCCGTAAACTG+CGG 0.641216 3.1:-84680708 None:intergenic
GGAGGACTTGGTGAATCTGA+GGG 0.641575 3.1:+84680719 MS.gene32646:CDS
TAAACAGATTGATGAACTTG+TGG 0.645164 3.1:+84680416 MS.gene32646:CDS
AGCTTGAACAAAATGAACAA+TGG 0.645990 3.1:+84681293 MS.gene32646:CDS
CCATACTCATACTCATTCGG+TGG 0.649853 3.1:-84680030 None:intergenic
TGAAAACTCTCCTTAACCAA+CGG 0.650974 3.1:-84680288 None:intergenic
GAGAGCTAAAACAGGTGTTG+TGG 0.651335 3.1:-84681096 None:intergenic
GCTGTATGCTGATATATGCG+AGG 0.654854 3.1:+84680311 MS.gene32646:CDS
AGCGAAAGTAGCGAGCAACA+TGG 0.657386 3.1:+84679546 MS.gene32646:CDS
TGAGGGATATGAAGCCGGAG+AGG 0.659064 3.1:+84680160 MS.gene32646:CDS
CCGAATGAGTATGAGTATGG+AGG 0.662053 3.1:+84680033 MS.gene32646:CDS
GCACTTCCCAACTTAGTGGA+GGG 0.664190 3.1:+84681036 MS.gene32646:CDS
TAAGTTGTTGGAGACACTGG+AGG 0.669834 3.1:+84680512 MS.gene32646:CDS
GCTGTGGCATCAGTATGCAA+GGG 0.669933 3.1:+84681250 MS.gene32646:CDS
AGGCAGAAGAATGCTTTAGG+TGG 0.677279 3.1:+84680951 MS.gene32646:CDS
TGGCATGTATGATCAAGGAA+TGG 0.678938 3.1:+84680998 MS.gene32646:CDS
TTGAACTTCCGGATACTCGG+AGG 0.680206 3.1:-84680478 None:intergenic
GGACTTGGTGAATCTGAGGG+AGG 0.680416 3.1:+84680722 MS.gene32646:CDS
TGGAGGGAGTGCTAGCAGTG+TGG 0.687452 3.1:+84681052 MS.gene32646:CDS
AGGTCCCATTCCATGAACTG+GGG 0.690448 3.1:-84681357 None:intergenic
GGTACTGGATACTATGCTGG+GGG 0.690788 3.1:+84681314 MS.gene32646:CDS
GGAGTATCCTGATTGTGTGA+AGG 0.692728 3.1:+84680368 MS.gene32646:CDS
GCCAAAACCGCAGTTTACGG+AGG 0.695554 3.1:+84680701 MS.gene32646:CDS
GTGATGTGAATGAAGTGTCG+GGG 0.703090 3.1:+84680103 MS.gene32646:CDS
TGGCTGCACAGATCCATCTG+GGG 0.707777 3.1:-84681123 None:intergenic
AATCCGAAAAGCGATTGGAG+CGG 0.709364 3.1:+84679498 MS.gene32646:CDS
AAGGTGGGATGAGGAAGACG+AGG 0.712639 3.1:+84680073 MS.gene32646:CDS
AGAGGCCAAAGAGTCCAGAG+AGG 0.718357 3.1:+84680556 MS.gene32646:CDS
TGTATGATCAAGGAATGGTG+AGG 0.740576 3.1:+84681003 MS.gene32646:CDS
AAGAGATTATGTATGCTACG+AGG 0.775372 3.1:+84679800 MS.gene32646:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGAATTACTAGTAAGTTGT+TGG + chr3.1:84680500-84680519 MS.gene32646:CDS 25.0%
AACAAAATGAACAATGGTAC+TGG + chr3.1:84681299-84681318 MS.gene32646:CDS 30.0%
AGCTTGAACAAAATGAACAA+TGG + chr3.1:84681293-84681312 MS.gene32646:CDS 30.0%
ATAATAATGCAAATGGATCG+TGG + chr3.1:84680811-84680830 MS.gene32646:CDS 30.0%
ATAATCTCTTCCTGGAAAAT+CGG - chr3.1:84679790-84679809 None:intergenic 30.0%
ATACATGCCATTCAACAATA+AGG - chr3.1:84680988-84681007 None:intergenic 30.0%
CCATTTGCATTATTATTAGC+CGG - chr3.1:84680807-84680826 None:intergenic 30.0%
CTAGTAATTCTTTGAACTTC+CGG - chr3.1:84680492-84680511 None:intergenic 30.0%
TAAACAGATTGATGAACTTG+TGG + chr3.1:84680416-84680435 MS.gene32646:CDS 30.0%
TAATAATGCAAATGGATCGT+GGG + chr3.1:84680812-84680831 MS.gene32646:CDS 30.0%
TACAATTGGTGTAAAGAAAG+TGG + chr3.1:84680444-84680463 MS.gene32646:CDS 30.0%
TACATGCCATTCAACAATAA+GGG - chr3.1:84680987-84681006 None:intergenic 30.0%
! ATGTTGTTTGATAGAAAAGC+TGG + chr3.1:84679940-84679959 MS.gene32646:CDS 30.0%
!! TATGTCTGATTTTAGAGATG+AGG + chr3.1:84679843-84679862 MS.gene32646:CDS 30.0%
AAGAGATTATGTATGCTACG+AGG + chr3.1:84679800-84679819 MS.gene32646:CDS 35.0%
CCGGCTAATAATAATGCAAA+TGG + chr3.1:84680804-84680823 MS.gene32646:CDS 35.0%
CTTGATCCCTTATTGTTGAA+TGG + chr3.1:84680978-84680997 MS.gene32646:CDS 35.0%
GCTTGACAAATTCTTTGACA+TGG + chr3.1:84680347-84680366 MS.gene32646:CDS 35.0%
GTGGGAGAGAAAATAATTTC+AGG + chr3.1:84680004-84680023 MS.gene32646:CDS 35.0%
TAGCATACATAATCTCTTCC+TGG - chr3.1:84679798-84679817 None:intergenic 35.0%
TGAAAACTCTCCTTAACCAA+CGG - chr3.1:84680291-84680310 None:intergenic 35.0%
TTGAATGGCATGTATGATCA+AGG + chr3.1:84680993-84681012 MS.gene32646:CDS 35.0%
TTGGTGTAAAGAAAGTGGTT+TGG + chr3.1:84680449-84680468 MS.gene32646:CDS 35.0%
TTTGATTGCATTGTATCCGT+TGG + chr3.1:84680272-84680291 MS.gene32646:CDS 35.0%
! AACTTGTGGCGTTTTACAAT+TGG + chr3.1:84680430-84680449 MS.gene32646:CDS 35.0%
! TCATTTTGTTCAAGCTAGAC+TGG - chr3.1:84681289-84681308 None:intergenic 35.0%
! TTGTTGCTTCTTCTCCATTT+CGG - chr3.1:84681210-84681229 None:intergenic 35.0%
AATGAGTATGAGTATGGAGG+TGG + chr3.1:84680036-84680055 MS.gene32646:CDS 40.0%
AGACAGCCAGCAATTTATCT+AGG + chr3.1:84680931-84680950 MS.gene32646:CDS 40.0%
AGCACTTGCATAAGCATCAA+AGG - chr3.1:84680394-84680413 None:intergenic 40.0%
AGGTGGAATGTTCAATGATG+CGG - chr3.1:84681168-84681187 None:intergenic 40.0%
ATCTCGTTCATATCCTGAAC+CGG - chr3.1:84680621-84680640 None:intergenic 40.0%
ATGCTTTGTATCTCGATCAG+AGG + chr3.1:84679908-84679927 MS.gene32646:CDS 40.0%
ATGGAGGTGGACAATTTAGA+GGG + chr3.1:84680049-84680068 MS.gene32646:CDS 40.0%
ATTATGTATGCTACGAGGAG+AGG + chr3.1:84679805-84679824 MS.gene32646:CDS 40.0%
CCTCCATACTCATACTCATT+CGG - chr3.1:84680036-84680055 None:intergenic 40.0%
CTTATATACTTCTCACTCGC+CGG - chr3.1:84679622-84679641 None:intergenic 40.0%
CTTCTGCCTAGATAAATTGC+TGG - chr3.1:84680940-84680959 None:intergenic 40.0%
TAGTAAGTTGTTGGAGACAC+TGG + chr3.1:84680509-84680528 MS.gene32646:CDS 40.0%
TATGGAGGTGGACAATTTAG+AGG + chr3.1:84680048-84680067 MS.gene32646:CDS 40.0%
TCAGGATATGAACGAGATCA+AGG + chr3.1:84680623-84680642 MS.gene32646:CDS 40.0%
TCAGGGTAATAGATTTGCGT+TGG + chr3.1:84680764-84680783 MS.gene32646:CDS 40.0%
TCTAGGCAGAAGAATGCTTT+AGG + chr3.1:84680948-84680967 MS.gene32646:CDS 40.0%
TCTTTGAACTTCCGGATACT+CGG - chr3.1:84680484-84680503 None:intergenic 40.0%
TGGCATGTATGATCAAGGAA+TGG + chr3.1:84680998-84681017 MS.gene32646:CDS 40.0%
TGTATGATCAAGGAATGGTG+AGG + chr3.1:84681003-84681022 MS.gene32646:CDS 40.0%
TGTTACTGTGACTCCTTTGA+GGG + chr3.1:84680143-84680162 MS.gene32646:CDS 40.0%
TTATGTATGCTACGAGGAGA+GGG + chr3.1:84679806-84679825 MS.gene32646:CDS 40.0%
TTGTTACTGTGACTCCTTTG+AGG + chr3.1:84680142-84680161 MS.gene32646:CDS 40.0%
! AATGGTACTGGATACTATGC+TGG + chr3.1:84681311-84681330 MS.gene32646:CDS 40.0%
! AGAAGAATGCTTTAGGTGGT+GGG + chr3.1:84680955-84680974 MS.gene32646:CDS 40.0%
! ATGAGGCTCATTCAAGTTCT+TGG + chr3.1:84679860-84679879 MS.gene32646:CDS 40.0%
! ATGGTACTGGATACTATGCT+GGG + chr3.1:84681312-84681331 MS.gene32646:CDS 40.0%
! GATAGAAAAGCTGGTAGTGT+TGG + chr3.1:84679949-84679968 MS.gene32646:CDS 40.0%
! GTTCATCGACTGATGAATGA+TGG + chr3.1:84679760-84679779 MS.gene32646:CDS 40.0%
! TGAGGCTCATTCAAGTTCTT+GGG + chr3.1:84679861-84679880 MS.gene32646:CDS 40.0%
! TGCATTGTATCCGTTGGTTA+AGG + chr3.1:84680278-84680297 MS.gene32646:CDS 40.0%
! TGCTGATATATGCGAGGTTT+TGG + chr3.1:84680317-84680336 MS.gene32646:CDS 40.0%
! TGGTGATGTGAATGAAGTGT+CGG + chr3.1:84680101-84680120 MS.gene32646:CDS 40.0%
!! GGGATCATTCTGCTTTTGTT+AGG + chr3.1:84679881-84679900 MS.gene32646:CDS 40.0%
AAAGAGTCCAGAGAGGAAAG+AGG + chr3.1:84680563-84680582 MS.gene32646:CDS 45.0%
AACTTGATGTCTTACTCGCG+TGG + chr3.1:84679652-84679671 MS.gene32646:CDS 45.0%
AATCCGAAAAGCGATTGGAG+CGG + chr3.1:84679498-84679517 MS.gene32646:CDS 45.0%
AGCACAATCCGAAAAGCGAT+TGG + chr3.1:84679493-84679512 MS.gene32646:CDS 45.0%
ATCTGTGCAGCCAGTTAATC+AGG + chr3.1:84681133-84681152 MS.gene32646:CDS 45.0%
ATCTTCCCGAAAATCCTCTC+CGG - chr3.1:84680177-84680196 None:intergenic 45.0%
ATGTTCAATGATGCGGCGAA+CGG - chr3.1:84681161-84681180 None:intergenic 45.0%
CATTTCGGCCATTTGCACAT+AGG - chr3.1:84681195-84681214 None:intergenic 45.0%
CCACCGAATGAGTATGAGTA+TGG + chr3.1:84680030-84680049 MS.gene32646:CDS 45.0%
CCATACTCATACTCATTCGG+TGG - chr3.1:84680033-84680052 None:intergenic 45.0%
CCGAATGAGTATGAGTATGG+AGG + chr3.1:84680033-84680052 MS.gene32646:CDS 45.0%
CTATGTGCAAATGGCCGAAA+TGG + chr3.1:84681193-84681212 MS.gene32646:CDS 45.0%
CTTTCCATCAAATGGACAGC+CGG + chr3.1:84680839-84680858 MS.gene32646:CDS 45.0%
GACAATTTAGAGGGGAAGGT+GGG + chr3.1:84680058-84680077 MS.gene32646:CDS 45.0%
GCTGTATGCTGATATATGCG+AGG + chr3.1:84680311-84680330 MS.gene32646:CDS 45.0%
GGAAGATGAGTCATTTGCAG+AGG + chr3.1:84680190-84680209 MS.gene32646:CDS 45.0%
GGAGTATGGTAATACCCGTT+TGG - chr3.1:84681383-84681402 None:intergenic 45.0%
GTATGGTAATACCCGTTTGG+AGG - chr3.1:84681380-84681399 None:intergenic 45.0%
GTGAAGGATCAGACGAGTAT+TGG + chr3.1:84679520-84679539 MS.gene32646:CDS 45.0%
TAAGTTGTTGGAGACACTGG+AGG + chr3.1:84680512-84680531 MS.gene32646:CDS 45.0%
TCAAAGGCCTTCACACAATC+AGG - chr3.1:84680378-84680397 None:intergenic 45.0%
TCCTCTTGTACTTCCAGCTT+AGG - chr3.1:84680594-84680613 None:intergenic 45.0%
TCTTTCCTCTCTGGACTCTT+TGG - chr3.1:84680564-84680583 None:intergenic 45.0%
TGAGCCAAAACCGCAGTTTA+CGG + chr3.1:84680698-84680717 MS.gene32646:CDS 45.0%
TGCCAAGCTGAAGTTACTTC+CGG - chr3.1:84680861-84680880 None:intergenic 45.0%
TGGAGGTGGACAATTTAGAG+GGG + chr3.1:84680050-84680069 MS.gene32646:CDS 45.0%
TGGGAAGCCTTTCCATCAAA+TGG + chr3.1:84680831-84680850 MS.gene32646:CDS 45.0%
TGTCGGGGAATGATGATAGA+AGG + chr3.1:84680118-84680137 MS.gene32646:CDS 45.0%
TTGTTCTTAGCCAACCCAGT+TGG - chr3.1:84680243-84680262 None:intergenic 45.0%
! AGAAAAGCTGGTAGTGTTGG+TGG + chr3.1:84679952-84679971 MS.gene32646:CDS 45.0%
! AGGCAGAAGAATGCTTTAGG+TGG + chr3.1:84680951-84680970 MS.gene32646:CDS 45.0%
! ATGAAGCCGGAGAGGATTTT+CGG + chr3.1:84680168-84680187 MS.gene32646:CDS 45.0%
! CAGAAGAATGCTTTAGGTGG+TGG + chr3.1:84680954-84680973 MS.gene32646:CDS 45.0%
! GAGAGCTAAAACAGGTGTTG+TGG - chr3.1:84681099-84681118 None:intergenic 45.0%
! GAGGCTTTTGGATCGTTTCT+TGG + chr3.1:84680209-84680228 MS.gene32646:CDS 45.0%
! GAGTCATTTGCAGAGGCTTT+TGG + chr3.1:84680197-84680216 MS.gene32646:CDS 45.0%
! GGAGTATCCTGATTGTGTGA+AGG + chr3.1:84680368-84680387 MS.gene32646:CDS 45.0%
! GGTGATGTGAATGAAGTGTC+GGG + chr3.1:84680102-84680121 MS.gene32646:CDS 45.0%
! TAAGCTGGAAGTACAAGAGG+AGG + chr3.1:84680593-84680612 MS.gene32646:CDS 45.0%
! TCCTAAGCTGGAAGTACAAG+AGG + chr3.1:84680590-84680609 MS.gene32646:CDS 45.0%
! TCCTTTGAGGGATATGAAGC+CGG + chr3.1:84680155-84680174 MS.gene32646:CDS 45.0%
! TGGTACTGGATACTATGCTG+GGG + chr3.1:84681313-84681332 MS.gene32646:CDS 45.0%
!! CTGGGTTGGCTAAGAACAAT+AGG + chr3.1:84680244-84680263 MS.gene32646:CDS 45.0%
!! GTGATGTGAATGAAGTGTCG+GGG + chr3.1:84680103-84680122 MS.gene32646:CDS 45.0%
!! TGGATCGTTTCTTGGCTTGT+AGG + chr3.1:84680217-84680236 MS.gene32646:CDS 45.0%
!!! TGATGGAACACCGATTTTCC+AGG + chr3.1:84679777-84679796 MS.gene32646:CDS 45.0%
AAAAGCGATTGGAGCGGTGA+AGG + chr3.1:84679504-84679523 MS.gene32646:CDS 50.0%
AATGCCCCCAGTTCATGGAA+TGG + chr3.1:84681352-84681371 MS.gene32646:CDS 50.0%
ACAATCGCCACCTCCAATTC+CGG - chr3.1:84679574-84679593 None:intergenic 50.0%
ACCAAGTCCTCCGTAAACTG+CGG - chr3.1:84680711-84680730 None:intergenic 50.0%
ACCTCCCTCCTATGTGCAAA+TGG + chr3.1:84681184-84681203 MS.gene32646:CDS 50.0%
ACTTCCGGCTGTCCATTTGA+TGG - chr3.1:84680846-84680865 None:intergenic 50.0%
AGACACTGGAGGAGTTTGTG+AGG + chr3.1:84680523-84680542 MS.gene32646:CDS 50.0%
AGCACTCCCTCCACTAAGTT+GGG - chr3.1:84681045-84681064 None:intergenic 50.0%
AGCACTTCCCAACTTAGTGG+AGG + chr3.1:84681035-84681054 MS.gene32646:CDS 50.0%
AGCCGGAAGTAACTTCAGCT+TGG + chr3.1:84680856-84680875 MS.gene32646:CDS 50.0%
AGCGAAAGTAGCGAGCAACA+TGG + chr3.1:84679546-84679565 MS.gene32646:CDS 50.0%
AGCTGTGGCATCAGTATGCA+AGG + chr3.1:84681249-84681268 MS.gene32646:CDS 50.0%
AGGTCCCATTCCATGAACTG+GGG - chr3.1:84681360-84681379 None:intergenic 50.0%
ATGAGGAAGACGAGGTCGTA+TGG + chr3.1:84680081-84680100 MS.gene32646:CDS 50.0%
ATGCCCCCAGTTCATGGAAT+GGG + chr3.1:84681353-84681372 MS.gene32646:CDS 50.0%
ATGGAATGGGACCTCCAAAC+GGG + chr3.1:84681366-84681385 MS.gene32646:CDS 50.0%
ATTTGCACATAGGAGGGAGG+TGG - chr3.1:84681185-84681204 None:intergenic 50.0%
CATGGAATGGGACCTCCAAA+CGG + chr3.1:84681365-84681384 MS.gene32646:CDS 50.0%
CGGCTGTCCATTTGATGGAA+AGG - chr3.1:84680841-84680860 None:intergenic 50.0%
GAAAGAGGAAGCTCCTAAGC+TGG + chr3.1:84680578-84680597 MS.gene32646:CDS 50.0%
GACACTGGAGGAGTTTGTGA+GGG + chr3.1:84680524-84680543 MS.gene32646:CDS 50.0%
GAGGTCCCATTCCATGAACT+GGG - chr3.1:84681361-84681380 None:intergenic 50.0%
GCACTTCCCAACTTAGTGGA+GGG + chr3.1:84681036-84681055 MS.gene32646:CDS 50.0%
GCGAACGGATCCTGATTAAC+TGG - chr3.1:84681146-84681165 None:intergenic 50.0%
GCTGTGGCATCAGTATGCAA+GGG + chr3.1:84681250-84681269 MS.gene32646:CDS 50.0%
GGACAATTTAGAGGGGAAGG+TGG + chr3.1:84680057-84680076 MS.gene32646:CDS 50.0%
GGAGGAGTTTGTGAGGGATA+GGG + chr3.1:84680530-84680549 MS.gene32646:CDS 50.0%
GGCATCAGTATGCAAGGGAT+GGG + chr3.1:84681255-84681274 MS.gene32646:CDS 50.0%
GGGGTATAATTCTCAGGTGC+AGG - chr3.1:84680654-84680673 None:intergenic 50.0%
GGTGGACAATTTAGAGGGGA+AGG + chr3.1:84680054-84680073 MS.gene32646:CDS 50.0%
GTGAGCACTTCCCAACTTAG+TGG + chr3.1:84681032-84681051 MS.gene32646:CDS 50.0%
TAGCACTCCCTCCACTAAGT+TGG - chr3.1:84681046-84681065 None:intergenic 50.0%
TATGCAAGGGATGGGATGCA+AGG + chr3.1:84681263-84681282 MS.gene32646:CDS 50.0%
TATGGAATGCCCCCAGTTCA+TGG + chr3.1:84681347-84681366 MS.gene32646:CDS 50.0%
TCAGCTGTGTCCAAGCGATT+GGG + chr3.1:84679694-84679713 MS.gene32646:CDS 50.0%
TCCGGCTTCATATCCCTCAA+AGG - chr3.1:84680159-84680178 None:intergenic 50.0%
TCGGCCATTTGCACATAGGA+GGG - chr3.1:84681191-84681210 None:intergenic 50.0%
TGGAGGAGTTTGTGAGGGAT+AGG + chr3.1:84680529-84680548 MS.gene32646:CDS 50.0%
TGGCATCAGTATGCAAGGGA+TGG + chr3.1:84681254-84681273 MS.gene32646:CDS 50.0%
TTCAGCTGTGTCCAAGCGAT+TGG + chr3.1:84679693-84679712 MS.gene32646:CDS 50.0%
TTCGGCCATTTGCACATAGG+AGG - chr3.1:84681192-84681211 None:intergenic 50.0%
TTGAACTTCCGGATACTCGG+AGG - chr3.1:84680481-84680500 None:intergenic 50.0%
TTGTGAGGGATAGGGCTAAG+AGG + chr3.1:84680538-84680557 MS.gene32646:CDS 50.0%
! ACAGGTGTTGTGGTCTTTCC+CGG - chr3.1:84681089-84681108 None:intergenic 50.0%
! GGTACTGGATACTATGCTGG+GGG + chr3.1:84681314-84681333 MS.gene32646:CDS 50.0%
! TCCTCCGTAAACTGCGGTTT+TGG - chr3.1:84680705-84680724 None:intergenic 50.0%
! TGAACCTGGGAAAGCGGATT+GGG + chr3.1:84680893-84680912 MS.gene32646:CDS 50.0%
! TGAAGCCGGAGAGGATTTTC+GGG + chr3.1:84680169-84680188 MS.gene32646:CDS 50.0%
! TTTGCGTTGGCACTCTTTGC+TGG + chr3.1:84680777-84680796 MS.gene32646:CDS 50.0%
!! AAAGCTGGTAGTGTTGGTGG+TGG + chr3.1:84679955-84679974 MS.gene32646:CDS 50.0%
!! GGAGGACTTGGTGAATCTGA+GGG + chr3.1:84680719-84680738 MS.gene32646:CDS 50.0%
!! TCACCGCTCCAATCGCTTTT+CGG - chr3.1:84679504-84679523 None:intergenic 50.0%
!! TCTTGGCTTGTAGGCCAACT+GGG + chr3.1:84680226-84680245 MS.gene32646:CDS 50.0%
!! TGAACTGGGGGCATTCCATA+AGG - chr3.1:84681347-84681366 None:intergenic 50.0%
!! TTCTTGGCTTGTAGGCCAAC+TGG + chr3.1:84680225-84680244 MS.gene32646:CDS 50.0%
!!! GTAAACTGCGGTTTTGGCTC+AGG - chr3.1:84680699-84680718 None:intergenic 50.0%
AAACCCCTGCAGCTGAACCT+GGG + chr3.1:84680880-84680899 MS.gene32646:CDS 55.0%
AAGGTGGGATGAGGAAGACG+AGG + chr3.1:84680073-84680092 MS.gene32646:CDS 55.0%
ACCGCAGTTTACGGAGGACT+TGG + chr3.1:84680707-84680726 MS.gene32646:CDS 55.0%
ACTGGCTGCACAGATCCATC+TGG - chr3.1:84681128-84681147 None:intergenic 55.0%
AGAGGCCAAAGAGTCCAGAG+AGG + chr3.1:84680556-84680575 MS.gene32646:CDS 55.0%
AGTTCCCAATCCGCTTTCCC+AGG - chr3.1:84680900-84680919 None:intergenic 55.0%
ATGCTACGAGGAGAGGGACT+AGG + chr3.1:84679812-84679831 MS.gene32646:CDS 55.0%
CAGCTGTGTCCAAGCGATTG+GGG + chr3.1:84679695-84679714 MS.gene32646:CDS 55.0%
CTGAACCTGGGAAAGCGGAT+TGG + chr3.1:84680892-84680911 MS.gene32646:CDS 55.0%
CTGGCTGCACAGATCCATCT+GGG - chr3.1:84681127-84681146 None:intergenic 55.0%
CTTTCCCAGGTTCAGCTGCA+GGG - chr3.1:84680887-84680906 None:intergenic 55.0%
GACGAGTCACGACGAAGATC+CGG + chr3.1:84679600-84679619 MS.gene32646:CDS 55.0%
GATTGGGGAAGACGCGTGAT+TGG + chr3.1:84679710-84679729 MS.gene32646:CDS 55.0%
GCAGTCACTGCAGACGATCA+GGG + chr3.1:84680747-84680766 MS.gene32646:CDS 55.0%
GCCAAAACCGCAGTTTACGG+AGG + chr3.1:84680701-84680720 MS.gene32646:CDS 55.0%
GCCATTTGCACATAGGAGGG+AGG - chr3.1:84681188-84681207 None:intergenic 55.0%
GGAGCTTCCTCTTTCCTCTC+TGG - chr3.1:84680573-84680592 None:intergenic 55.0%
GGAGGTCCCATTCCATGAAC+TGG - chr3.1:84681362-84681381 None:intergenic 55.0%
GGTCCCATTCCATGAACTGG+GGG - chr3.1:84681359-84681378 None:intergenic 55.0%
GTGGGGATGAGAGGTTCAGT+GGG + chr3.1:84679986-84680005 MS.gene32646:CDS 55.0%
TGAGGGATATGAAGCCGGAG+AGG + chr3.1:84680160-84680179 MS.gene32646:CDS 55.0%
TGCAGCTGAACCTGGGAAAG+CGG + chr3.1:84680887-84680906 MS.gene32646:CDS 55.0%
TGCAGTCACTGCAGACGATC+AGG + chr3.1:84680746-84680765 MS.gene32646:CDS 55.0%
TGGCTGCACAGATCCATCTG+GGG - chr3.1:84681126-84681145 None:intergenic 55.0%
TTAGAGGGGAAGGTGGGATG+AGG + chr3.1:84680064-84680083 MS.gene32646:CDS 55.0%
TTGTGCATGAGCAGCAGCTG+TGG + chr3.1:84681234-84681253 MS.gene32646:CDS 55.0%
TTTCCCAGGTTCAGCTGCAG+GGG - chr3.1:84680886-84680905 None:intergenic 55.0%
! GGAAGTACAAGAGGAGGAGC+CGG + chr3.1:84680599-84680618 MS.gene32646:CDS 55.0%
!! AGCGGATTGGGAACTGGCAT+TGG + chr3.1:84680905-84680924 MS.gene32646:CDS 55.0%
!! CGGAGGACTTGGTGAATCTG+AGG + chr3.1:84680718-84680737 MS.gene32646:CDS 55.0%
!! GGACTTGGTGAATCTGAGGG+AGG + chr3.1:84680722-84680741 MS.gene32646:CDS 55.0%
!! GGGGGCATTCCATAAGGCAT+AGG - chr3.1:84681341-84681360 None:intergenic 55.0%
!! GGTGGCGGGGTATAATTCTC+AGG - chr3.1:84680660-84680679 None:intergenic 55.0%
!! TGGGAAAGCGGATTGGGAAC+TGG + chr3.1:84680899-84680918 MS.gene32646:CDS 55.0%
ACGCGTCTTCCCCAATCGCT+TGG - chr3.1:84679707-84679726 None:intergenic 60.0%
AGTAGCGAGCAACATGGCGC+CGG + chr3.1:84679552-84679571 MS.gene32646:CDS 60.0%
CAAACCCCTGCAGCTGAACC+TGG + chr3.1:84680879-84680898 MS.gene32646:CDS 60.0%
GCTGGGGGTCCTATGCCTTA+TGG + chr3.1:84681329-84681348 MS.gene32646:CDS 60.0%
GCTTTCCCAGGTTCAGCTGC+AGG - chr3.1:84680888-84680907 None:intergenic 60.0%
GGCACTCTTTGCTGGTGCAC+CGG + chr3.1:84680785-84680804 MS.gene32646:CDS 60.0%
GGCGAGATCCTCCGAGTATC+CGG + chr3.1:84680470-84680489 MS.gene32646:CDS 60.0%
GGCTTGTAGGCCAACTGGGT+TGG + chr3.1:84680230-84680249 MS.gene32646:CDS 60.0%
GGGGCTGGGAGAGCTAAAAC+AGG - chr3.1:84681107-84681126 None:intergenic 60.0%
GGTGGGGATGAGAGGTTCAG+TGG + chr3.1:84679985-84680004 MS.gene32646:CDS 60.0%
GTTGTGGTCTTTCCCGGTCC+CGG - chr3.1:84681083-84681102 None:intergenic 60.0%
TGCTAGCAGTGTGGCGTTGC+CGG + chr3.1:84681061-84681080 MS.gene32646:CDS 60.0%
! ACAAGAGGAGGAGCCGGTTC+AGG + chr3.1:84680605-84680624 MS.gene32646:CDS 60.0%
! CAACATGGCGCCGGAATTGG+AGG + chr3.1:84679561-84679580 MS.gene32646:CDS 60.0%
! GAGCAACATGGCGCCGGAAT+TGG + chr3.1:84679558-84679577 MS.gene32646:CDS 60.0%
! GCACAGATCCATCTGGGGCT+GGG - chr3.1:84681121-84681140 None:intergenic 60.0%
! TGCACAGATCCATCTGGGGC+TGG - chr3.1:84681122-84681141 None:intergenic 60.0%
! TTAGCTCTCCCAGCCCCAGA+TGG + chr3.1:84681110-84681129 MS.gene32646:CDS 60.0%
! TTTGGCTCAGGCTCAGGCTC+TGG - chr3.1:84680687-84680706 None:intergenic 60.0%
!! AGTGTTGGTGGTGGTGGTGG+AGG + chr3.1:84679964-84679983 MS.gene32646:CDS 60.0%
!! GCTGGTAGTGTTGGTGGTGG+TGG + chr3.1:84679958-84679977 MS.gene32646:CDS 60.0%
!! GGTAGTGTTGGTGGTGGTGG+TGG + chr3.1:84679961-84679980 MS.gene32646:CDS 60.0%
!! TGGAGGGAGTGCTAGCAGTG+TGG + chr3.1:84681052-84681071 MS.gene32646:CDS 60.0%
!! TTGGTGGTGGTGGTGGAGGT+GGG + chr3.1:84679968-84679987 MS.gene32646:CDS 60.0%
!!! TGCGGTTTTGGCTCAGGCTC+AGG - chr3.1:84680693-84680712 None:intergenic 60.0%
GCTAGCAGTGTGGCGTTGCC+GGG + chr3.1:84681062-84681081 MS.gene32646:CDS 65.0%
! CATGGCGCCGGAATTGGAGG+TGG + chr3.1:84679564-84679583 MS.gene32646:CDS 65.0%
! GTGGTGGAGGTGGGGATGAG+AGG + chr3.1:84679977-84679996 MS.gene32646:CDS 65.0%
! TCAGGCTCAGGCTCTGGTGG+AGG - chr3.1:84680681-84680700 None:intergenic 65.0%
! TCAGGCTCTGGTGGAGGTGG+CGG - chr3.1:84680675-84680694 None:intergenic 65.0%
!! GTTGGTGGTGGTGGTGGAGG+TGG + chr3.1:84679967-84679986 MS.gene32646:CDS 65.0%
!! TGGTGGTGGTGGTGGAGGTG+GGG + chr3.1:84679969-84679988 MS.gene32646:CDS 65.0%
AGTGTGGCGTTGCCGGGACC+GGG + chr3.1:84681068-84681087 MS.gene32646:CDS 70.0%
CAGTGTGGCGTTGCCGGGAC+CGG + chr3.1:84681067-84681086 MS.gene32646:CDS 70.0%
! AGGCTCTGGTGGAGGTGGCG+GGG - chr3.1:84680673-84680692 None:intergenic 70.0%
! CAGGCTCTGGTGGAGGTGGC+GGG - chr3.1:84680674-84680693 None:intergenic 70.0%
! GGCTCAGGCTCAGGCTCTGG+TGG - chr3.1:84680684-84680703 None:intergenic 70.0%
! GGCTCAGGCTCTGGTGGAGG+TGG - chr3.1:84680678-84680697 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 84679484 84681409 84679484 ID=MS.gene32646
chr3.1 mRNA 84679484 84681409 84679484 ID=MS.gene32646.t1;Parent=MS.gene32646
chr3.1 exon 84679484 84681409 84679484 ID=MS.gene32646.t1.exon1;Parent=MS.gene32646.t1
chr3.1 CDS 84679484 84681409 84679484 ID=cds.MS.gene32646.t1;Parent=MS.gene32646.t1
Gene Sequence

>MS.gene32646

ATGACTTCGAGCACAATCCGAAAAGCGATTGGAGCGGTGAAGGATCAGACGAGTATTGGAATAGCGAAAGTAGCGAGCAACATGGCGCCGGAATTGGAGGTGGCGATTGTGAAAGCGACGAGTCACGACGAAGATCCGGCGAGTGAGAAGTATATAAGAGAGATCTTGAACTTGATGTCTTACTCGCGTGGTTATGTGAACGCGTGTGTTTCAGCTGTGTCCAAGCGATTGGGGAAGACGCGTGATTGGATTGTTGCGTTGAAAGCTCTTATACTTGTTCATCGACTGATGAATGATGGAACACCGATTTTCCAGGAAGAGATTATGTATGCTACGAGGAGAGGGACTAGGTTGTTGAATATGTCTGATTTTAGAGATGAGGCTCATTCAAGTTCTTGGGATCATTCTGCTTTTGTTAGGACTTATGCTTTGTATCTCGATCAGAGGCTTGAGTTGATGTTGTTTGATAGAAAAGCTGGTAGTGTTGGTGGTGGTGGTGGAGGTGGGGATGAGAGGTTCAGTGGGAGAGAAAATAATTTCAGGTCGCCACCGAATGAGTATGAGTATGGAGGTGGACAATTTAGAGGGGAAGGTGGGATGAGGAAGACGAGGTCGTATGGTGATGTGAATGAAGTGTCGGGGAATGATGATAGAAGGATTGTTACTGTGACTCCTTTGAGGGATATGAAGCCGGAGAGGATTTTCGGGAAGATGAGTCATTTGCAGAGGCTTTTGGATCGTTTCTTGGCTTGTAGGCCAACTGGGTTGGCTAAGAACAATAGGATGATTTTGATTGCATTGTATCCGTTGGTTAAGGAGAGTTTTCAGCTGTATGCTGATATATGCGAGGTTTTGGCAGTGTTGCTTGACAAATTCTTTGACATGGAGTATCCTGATTGTGTGAAGGCCTTTGATGCTTATGCAAGTGCTGCTAAACAGATTGATGAACTTGTGGCGTTTTACAATTGGTGTAAAGAAAGTGGTTTGGCGAGATCCTCCGAGTATCCGGAAGTTCAAAGAATTACTAGTAAGTTGTTGGAGACACTGGAGGAGTTTGTGAGGGATAGGGCTAAGAGGCCAAAGAGTCCAGAGAGGAAAGAGGAAGCTCCTAAGCTGGAAGTACAAGAGGAGGAGCCGGTTCAGGATATGAACGAGATCAAGGCACTGCCTGCACCTGAGAATTATACCCCGCCACCTCCACCAGAGCCTGAGCCTGAGCCAAAACCGCAGTTTACGGAGGACTTGGTGAATCTGAGGGAGGATGCAGTCACTGCAGACGATCAGGGTAATAGATTTGCGTTGGCACTCTTTGCTGGTGCACCGGCTAATAATAATGCAAATGGATCGTGGGAAGCCTTTCCATCAAATGGACAGCCGGAAGTAACTTCAGCTTGGCAAACCCCTGCAGCTGAACCTGGGAAAGCGGATTGGGAACTGGCATTGGTTGAGACAGCCAGCAATTTATCTAGGCAGAAGAATGCTTTAGGTGGTGGGCTTGATCCCTTATTGTTGAATGGCATGTATGATCAAGGAATGGTGAGGCAGCATGTGAGCACTTCCCAACTTAGTGGAGGGAGTGCTAGCAGTGTGGCGTTGCCGGGACCGGGAAAGACCACAACACCTGTTTTAGCTCTCCCAGCCCCAGATGGATCTGTGCAGCCAGTTAATCAGGATCCGTTCGCCGCATCATTGAACATTCCACCTCCCTCCTATGTGCAAATGGCCGAAATGGAGAAGAAGCAACAATTACTTGTGCATGAGCAGCAGCTGTGGCATCAGTATGCAAGGGATGGGATGCAAGGCCAGTCTAGCTTGAACAAAATGAACAATGGTACTGGATACTATGCTGGGGGTCCTATGCCTTATGGAATGCCCCCAGTTCATGGAATGGGACCTCCAAACGGGTATTACCATACTCCTATATGA
Protein sequence

>MS.gene32646.t1

MTSSTIRKAIGAVKDQTSIGIAKVASNMAPELEVAIVKATSHDEDPASEKYIREILNLMSYSRGYVNACVSAVSKRLGKTRDWIVALKALILVHRLMNDGTPIFQEEIMYATRRGTRLLNMSDFRDEAHSSSWDHSAFVRTYALYLDQRLELMLFDRKAGSVGGGGGGGDERFSGRENNFRSPPNEYEYGGGQFRGEGGMRKTRSYGDVNEVSGNDDRRIVTVTPLRDMKPERIFGKMSHLQRLLDRFLACRPTGLAKNNRMILIALYPLVKESFQLYADICEVLAVLLDKFFDMEYPDCVKAFDAYASAAKQIDELVAFYNWCKESGLARSSEYPEVQRITSKLLETLEEFVRDRAKRPKSPERKEEAPKLEVQEEEPVQDMNEIKALPAPENYTPPPPPEPEPEPKPQFTEDLVNLREDAVTADDQGNRFALALFAGAPANNNANGSWEAFPSNGQPEVTSAWQTPAAEPGKADWELALVETASNLSRQKNALGGGLDPLLLNGMYDQGMVRQHVSTSQLSGGSASSVALPGPGKTTTPVLALPAPDGSVQPVNQDPFAASLNIPPPSYVQMAEMEKKQQLLVHEQQLWHQYARDGMQGQSSLNKMNNGTGYYAGGPMPYGMPPVHGMGPPNGYYHTPI