AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33495

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33495.t1 MTR_4g015460 93.511 262 15 2 1 260 245 506 8.43e-179 501
MS.gene33495.t1 MTR_4g015420 82.031 256 44 1 1 254 269 524 1.12e-154 441
MS.gene33495.t1 MTR_2g043200 64.961 254 85 1 1 254 269 518 9.81e-114 337
MS.gene33495.t1 MTR_4g066330 66.406 256 76 5 1 254 245 492 2.16e-111 330
MS.gene33495.t1 MTR_4g066280 64.453 256 81 6 1 254 245 492 3.20e-107 319
MS.gene33495.t1 MTR_2g098520 59.766 256 91 4 1 254 264 509 3.77e-101 304
MS.gene33495.t1 MTR_2g098540 58.366 257 95 4 1 255 217 463 7.30e-98 294
MS.gene33495.t1 MTR_2g098540 58.366 257 95 4 1 255 261 507 1.44e-97 295
MS.gene33495.t1 MTR_2g098790 58.594 256 94 5 1 254 268 513 2.78e-97 294
MS.gene33495.t1 MTR_4g066340 56.977 258 99 4 1 256 193 440 1.72e-96 290
MS.gene33495.t1 MTR_3g028720 57.255 255 97 4 2 254 269 513 4.60e-93 284
MS.gene33495.t1 MTR_4g066240 53.488 258 108 3 1 256 266 513 2.32e-92 281
MS.gene33495.t1 MTR_4g066580 55.859 256 101 4 1 254 269 514 1.79e-91 280
MS.gene33495.t1 MTR_6g078330 55.385 260 104 4 1 258 163 412 2.98e-91 276
MS.gene33495.t1 MTR_4g066210 54.688 256 104 5 1 254 273 518 4.58e-91 278
MS.gene33495.t1 MTR_6g078330 55.385 260 104 4 1 258 163 412 4.76e-91 275
MS.gene33495.t1 MTR_4g066240 53.282 259 108 4 1 256 266 514 1.50e-90 277
MS.gene33495.t1 MTR_6g078330 55.385 260 104 4 1 258 267 516 6.27e-90 276
MS.gene33495.t1 MTR_4g066590 56.078 255 100 4 1 253 263 507 6.66e-90 275
MS.gene33495.t1 MTR_6g078450 53.077 260 110 3 1 258 266 515 1.43e-88 272
MS.gene33495.t1 MTR_5g071520 54.373 263 96 4 1 255 278 524 3.50e-88 271
MS.gene33495.t1 MTR_4g066640 53.488 258 107 5 2 256 267 514 5.03e-85 263
MS.gene33495.t1 MTR_4g066630 52.941 255 108 3 1 253 269 513 9.28e-85 262
MS.gene33495.t1 MTR_6g078380 54.054 259 108 3 2 258 267 516 3.09e-80 251
MS.gene33495.t1 MTR_4g023000 51.172 256 106 6 2 254 254 493 1.07e-73 233
MS.gene33495.t1 MTR_3g026280 49.416 257 113 5 1 255 252 493 2.08e-72 229
MS.gene33495.t1 MTR_3g026400 45.669 254 125 3 2 253 234 476 3.48e-67 216
MS.gene33495.t1 MTR_3g026400 45.669 254 125 3 2 253 265 507 7.07e-67 216
MS.gene33495.t1 MTR_3g026620 45.669 254 125 5 2 253 266 508 1.08e-66 215
MS.gene33495.t1 MTR_4g083000 42.802 257 133 4 2 254 162 408 1.61e-65 209
MS.gene33495.t1 MTR_4g083000 42.802 257 133 4 2 254 256 502 7.78e-65 210
MS.gene33495.t1 MTR_3g028720 55.224 201 78 4 2 200 269 459 1.81e-63 206
MS.gene33495.t1 MTR_5g044000 42.023 257 134 5 2 253 274 520 8.02e-63 205
MS.gene33495.t1 MTR_4g081530 44.015 259 128 5 2 254 252 499 8.21e-63 205
MS.gene33495.t1 MTR_3g033790 42.353 255 133 4 2 254 238 480 3.37e-61 200
MS.gene33495.t1 MTR_6g078450 50.990 202 87 3 1 200 266 457 5.07e-58 191
MS.gene33495.t1 MTR_4g131810 40.784 255 138 5 2 253 259 503 7.07e-58 192
MS.gene33495.t1 MTR_3g033810 40.784 255 138 5 2 254 240 483 1.96e-56 188
MS.gene33495.t1 MTR_2g022760 39.922 258 144 5 2 257 162 410 8.18e-55 182
MS.gene33495.t1 MTR_4g066630 48.756 201 91 3 1 199 269 459 8.48e-55 183
MS.gene33495.t1 MTR_2g022710 38.783 263 147 7 2 260 162 414 1.69e-54 181
MS.gene33495.t1 MTR_2g022760 39.922 258 144 5 2 257 265 513 7.32e-54 182
MS.gene33495.t1 MTR_2g022710 38.783 263 147 7 2 260 263 515 1.36e-53 181
MS.gene33495.t1 MTR_0110s0040 42.529 261 129 8 2 257 270 514 3.35e-53 180
MS.gene33495.t1 MTR_0110s0030 39.922 258 140 6 2 257 270 514 4.31e-51 174
MS.gene33495.t1 MTR_4g131800 40.310 258 143 5 2 257 744 992 9.18e-51 178
MS.gene33495.t1 MTR_2g079580 38.431 255 143 6 2 253 260 503 3.32e-50 172
MS.gene33495.t1 MTR_7g051650 37.354 257 141 7 1 253 288 528 2.47e-48 167
MS.gene33495.t1 MTR_7g051650 37.354 257 141 7 1 253 292 532 2.88e-48 167
MS.gene33495.t1 MTR_7g033860 37.308 260 141 6 2 256 272 514 7.44e-48 166
MS.gene33495.t1 MTR_2g079550 37.255 255 146 6 2 253 258 501 7.91e-48 166
MS.gene33495.t1 MTR_0108s0030 37.891 256 126 5 2 256 228 451 3.51e-47 163
MS.gene33495.t1 MTR_7g051610 36.576 257 146 5 2 256 293 534 7.31e-47 164
MS.gene33495.t1 MTR_7g051510 35.968 253 147 5 2 253 293 531 1.12e-46 163
MS.gene33495.t1 MTR_3g028720 52.096 167 68 4 2 166 269 425 2.32e-44 155
MS.gene33495.t1 MTR_7g056747 33.205 259 144 4 1 258 172 402 1.19e-43 152
MS.gene33495.t1 MTR_4g066580 50.595 168 71 4 1 166 269 426 4.19e-43 152
MS.gene33495.t1 MTR_4g066240 43.529 170 84 3 1 168 266 425 1.30e-38 139
MS.gene33495.t1 MTR_4g016920 35.686 255 141 5 2 253 162 396 2.37e-38 139
MS.gene33495.t1 MTR_4g016920 35.686 255 141 5 2 253 255 489 7.39e-38 139
MS.gene33495.t1 MTR_5g068740 32.171 258 154 4 2 257 254 492 1.49e-37 138
MS.gene33495.t1 MTR_4g066630 46.386 166 77 3 1 164 269 424 1.76e-37 137
MS.gene33495.t1 MTR_7g068910 32.549 255 151 5 2 254 259 494 1.87e-35 132
MS.gene33495.t1 MTR_7g068910 32.549 255 151 5 2 254 269 504 1.96e-35 132
MS.gene33495.t1 MTR_1g088610 31.765 255 152 4 2 254 254 488 4.46e-35 132
MS.gene33495.t1 MTR_1g088610 39.167 120 66 2 139 257 503 616 1.41e-19 88.2
MS.gene33495.t1 MTR_7g068900 33.591 259 144 6 2 254 183 419 1.07e-34 130
MS.gene33495.t1 MTR_4g067040 45.455 154 72 4 1 152 264 407 5.05e-32 122
MS.gene33495.t1 MTR_2g022710 34.653 202 120 5 2 200 263 455 7.28e-29 114
MS.gene33495.t1 MTR_2g022710 34.653 202 120 5 2 200 263 455 9.74e-29 114
MS.gene33495.t1 MTR_1g088630 30.973 226 133 5 34 257 231 435 4.15e-27 108
MS.gene33495.t1 MTR_3g028720 56.667 90 38 1 2 90 269 358 1.69e-26 106
MS.gene33495.t1 MTR_4g067040 54.839 93 41 1 1 92 264 356 3.26e-25 103
MS.gene33495.t1 MTR_2g022710 35.758 165 96 4 2 164 263 419 7.64e-25 102
MS.gene33495.t1 MTR_2g079580 34.940 166 95 5 2 164 260 415 1.10e-22 96.3
MS.gene33495.t1 MTR_5g069350 45.000 120 36 4 145 254 89 188 5.99e-22 90.5
MS.gene33495.t1 MTR_6g078330 48.387 93 47 1 1 92 267 359 1.20e-21 93.2
MS.gene33495.t1 MTR_4g066630 48.387 93 47 1 1 92 269 361 2.99e-21 92.0
MS.gene33495.t1 MTR_3g026400 50.575 87 42 1 2 87 265 351 1.83e-20 89.7
MS.gene33495.t1 MTR_4g066640 54.023 87 38 2 2 86 267 353 3.83e-20 89.0
MS.gene33495.t1 MTR_2g022710 33.333 153 92 4 2 152 263 407 2.00e-19 87.0
MS.gene33495.t1 MTR_0110s0040 45.263 95 49 2 2 93 270 364 2.19e-16 78.6
MS.gene33495.t1 MTR_4g016920 39.024 123 73 2 2 122 162 284 5.02e-16 76.6
MS.gene33495.t1 MTR_8g038650 42.697 89 50 1 2 89 62 150 2.16e-15 72.0
MS.gene33495.t1 MTR_2g022710 35.246 122 73 3 2 121 263 380 6.68e-15 73.9
MS.gene33495.t1 MTR_4g122980 31.658 199 89 7 62 258 384 537 7.33e-14 71.2
MS.gene33495.t1 MTR_4g061170 65.854 41 12 1 202 240 24 64 8.28e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33495.t1 AT2G44450 47.876 259 118 6 1 257 110 353 2.45e-73 228
MS.gene33495.t1 AT5G44640 47.692 260 118 6 1 257 261 505 1.72e-72 230
MS.gene33495.t1 AT2G44450 47.876 259 118 6 1 257 261 504 1.23e-71 228
MS.gene33495.t1 AT3G60130 47.266 256 117 5 2 254 209 449 3.36e-70 223
MS.gene33495.t1 AT3G60130 47.266 256 117 5 2 254 261 501 1.30e-69 223
MS.gene33495.t1 AT5G42260 48.016 252 113 6 1 249 261 497 9.00e-69 221
MS.gene33495.t1 AT5G54570 44.961 258 125 5 2 253 162 408 7.43e-68 216
MS.gene33495.t1 AT5G54570 44.961 258 125 5 2 253 259 505 5.23e-67 217
MS.gene33495.t1 AT3G60130 45.882 255 111 5 2 254 209 438 5.47e-66 212
MS.gene33495.t1 AT2G44480 41.245 257 139 3 2 256 59 305 2.44e-65 206
MS.gene33495.t1 AT5G36890 42.802 257 134 4 1 254 234 480 3.83e-64 208
MS.gene33495.t1 AT5G36890 42.802 257 134 4 1 254 234 480 4.61e-64 208
MS.gene33495.t1 AT1G26560 45.312 256 127 6 2 253 260 506 5.72e-64 208
MS.gene33495.t1 AT2G44480 41.245 257 139 3 2 256 163 409 7.11e-64 205
MS.gene33495.t1 AT2G44480 41.245 257 139 3 2 256 265 511 4.95e-63 206
MS.gene33495.t1 AT2G44480 41.245 257 139 3 2 256 296 542 8.59e-63 206
MS.gene33495.t1 AT2G44490 41.603 262 138 6 2 257 244 496 1.18e-62 206
MS.gene33495.t1 AT5G24540 44.015 259 135 5 1 256 261 512 2.97e-62 204
MS.gene33495.t1 AT3G18070 43.295 261 128 7 2 257 66 311 1.06e-61 197
MS.gene33495.t1 AT5G24550 43.893 262 131 5 1 256 261 512 1.28e-61 202
MS.gene33495.t1 AT3G18070 43.295 261 128 7 2 257 178 423 1.44e-60 197
MS.gene33495.t1 AT2G25630 44.314 255 109 6 1 253 260 483 5.76e-60 197
MS.gene33495.t1 AT3G18070 43.295 261 128 7 2 257 249 494 5.76e-60 197
MS.gene33495.t1 AT3G18070 43.295 261 128 7 2 257 255 500 8.16e-60 197
MS.gene33495.t1 AT3G60120 40.977 266 130 8 1 255 242 491 1.18e-59 197
MS.gene33495.t1 AT3G60140 43.243 259 132 6 1 254 255 503 6.22e-59 196
MS.gene33495.t1 AT3G18080 43.462 260 127 7 2 256 266 510 4.24e-58 193
MS.gene33495.t1 AT2G44460 43.411 258 133 5 1 254 111 359 8.82e-58 190
MS.gene33495.t1 AT2G44460 43.411 258 133 5 1 254 257 505 1.74e-56 190
MS.gene33495.t1 AT1G51470 43.307 254 129 6 2 253 269 509 1.88e-56 188
MS.gene33495.t1 AT1G47600 43.701 254 128 7 2 253 269 509 2.13e-56 188
MS.gene33495.t1 AT3G03640 43.191 257 131 6 4 254 266 513 2.56e-56 189
MS.gene33495.t1 AT1G61820 40.698 258 141 6 2 256 120 368 4.54e-56 184
MS.gene33495.t1 AT1G47600 43.701 254 127 8 2 253 269 508 5.16e-55 185
MS.gene33495.t1 AT1G61820 40.698 258 141 6 2 256 259 507 1.18e-54 184
MS.gene33495.t1 AT4G21760 40.000 260 140 6 2 259 282 527 6.79e-54 182
MS.gene33495.t1 AT1G61810 38.760 258 146 5 2 256 162 410 2.77e-51 173
MS.gene33495.t1 AT1G75940 40.698 258 142 6 1 254 268 518 2.89e-51 175
MS.gene33495.t1 AT2G44470 39.535 258 139 6 2 253 259 505 7.63e-51 175
MS.gene33495.t1 AT4G21760 38.996 259 134 6 2 259 282 517 8.03e-51 174
MS.gene33495.t1 AT1G61810 38.760 258 146 5 2 256 262 510 1.95e-50 173
MS.gene33495.t1 AT5G26000 40.392 255 139 5 2 253 267 511 1.96e-50 173
MS.gene33495.t1 AT1G66270 42.205 263 125 8 2 253 262 508 1.63e-49 170
MS.gene33495.t1 AT1G66270 42.205 263 125 8 2 253 264 510 1.87e-49 170
MS.gene33495.t1 AT3G21370 40.927 259 138 8 1 254 264 512 4.91e-49 169
MS.gene33495.t1 AT2G32860 37.165 261 149 6 4 259 327 577 7.78e-49 170
MS.gene33495.t1 AT1G66280 40.856 257 139 6 1 253 263 510 1.68e-48 168
MS.gene33495.t1 AT5G25980 40.467 257 138 9 2 254 278 523 3.15e-48 167
MS.gene33495.t1 AT5G28510 39.147 258 142 6 1 253 272 519 3.94e-48 167
MS.gene33495.t1 AT2G32860 35.849 265 145 8 4 259 328 576 6.17e-48 167
MS.gene33495.t1 AT1G52400 40.000 255 138 8 4 254 273 516 4.95e-47 164
MS.gene33495.t1 AT1G52400 40.000 255 138 8 4 254 273 516 4.95e-47 164
MS.gene33495.t1 AT3G09260 38.996 259 145 5 1 255 263 512 5.55e-47 164
MS.gene33495.t1 AT1G61810 39.149 235 132 4 2 233 262 488 1.02e-44 158
MS.gene33495.t1 AT5G54570 39.706 204 107 4 2 200 259 451 2.48e-41 147
MS.gene33495.t1 AT4G27820 35.433 254 144 6 2 253 162 397 3.59e-41 146
MS.gene33495.t1 AT4G27820 35.433 254 144 6 2 253 162 397 3.59e-41 146
MS.gene33495.t1 AT4G27830 35.827 254 142 6 2 253 162 396 3.60e-41 146
MS.gene33495.t1 AT4G27820 35.433 254 144 6 2 253 247 482 1.95e-40 146
MS.gene33495.t1 AT4G27830 35.827 254 142 6 2 253 250 484 1.96e-40 146
MS.gene33495.t1 AT1G02850 33.203 256 147 5 2 254 252 486 4.51e-38 139
MS.gene33495.t1 AT1G02850 33.203 256 147 5 2 254 275 509 4.56e-38 140
MS.gene33495.t1 AT3G62750 31.890 254 153 5 2 253 153 388 3.44e-37 135
MS.gene33495.t1 AT3G62750 31.890 254 153 5 2 253 153 388 3.44e-37 135
MS.gene33495.t1 AT4G22100 35.798 257 147 7 2 256 245 485 5.39e-37 137
MS.gene33495.t1 AT3G62750 31.890 254 153 5 2 253 238 473 1.30e-36 135
MS.gene33495.t1 AT3G62750 31.496 254 154 5 2 253 264 499 1.65e-36 135
MS.gene33495.t1 AT1G02850 33.074 257 147 6 2 254 275 510 2.36e-36 135
MS.gene33495.t1 AT1G02850 34.483 232 128 5 26 254 249 459 2.20e-35 132
MS.gene33495.t1 AT5G24540 38.806 201 114 4 1 199 261 454 5.94e-35 130
MS.gene33495.t1 AT3G62740 31.518 257 149 7 2 253 244 478 4.11e-33 126
MS.gene33495.t1 AT1G45191 32.549 255 148 5 2 254 252 484 2.78e-31 121
MS.gene33495.t1 AT5G26000 36.500 200 116 3 2 200 267 456 2.79e-30 117
MS.gene33495.t1 AT1G45191 32.157 255 160 5 2 254 264 507 4.39e-30 118
MS.gene33495.t1 AT1G45191 32.157 255 160 5 2 254 252 495 4.56e-30 117
MS.gene33495.t1 AT1G45191 32.157 255 155 6 2 254 252 490 5.42e-30 117
MS.gene33495.t1 AT5G48375 36.818 220 93 7 2 218 235 411 7.27e-30 116
MS.gene33495.t1 AT1G60090 31.765 255 154 6 2 254 247 483 1.30e-29 116
MS.gene33495.t1 AT5G25980 37.313 201 113 7 2 200 278 467 1.90e-29 115
MS.gene33495.t1 AT5G25980 37.313 201 113 7 2 200 278 467 1.90e-29 115
MS.gene33495.t1 AT3G18070 39.412 170 86 5 2 166 66 223 7.70e-29 109
MS.gene33495.t1 AT3G18070 39.412 170 86 5 2 166 178 335 7.31e-28 109
MS.gene33495.t1 AT2G44470 35.468 203 115 5 2 199 259 450 1.70e-27 110
MS.gene33495.t1 AT1G60090 30.651 261 156 7 2 254 247 490 1.77e-27 110
MS.gene33495.t1 AT3G60140 40.588 170 87 6 1 166 255 414 5.38e-26 105
MS.gene33495.t1 AT1G52400 35.644 202 116 7 1 199 270 460 2.00e-22 95.9
MS.gene33495.t1 AT5G16580 33.491 212 119 8 2 210 107 299 3.94e-22 93.2
MS.gene33495.t1 AT3G62750 27.723 202 127 4 2 202 264 447 4.55e-20 89.4
MS.gene33495.t1 AT1G61810 33.133 166 100 4 2 164 262 419 8.85e-20 88.2
MS.gene33495.t1 AT3G62740 30.256 195 110 6 2 192 244 416 8.96e-19 85.5
MS.gene33495.t1 AT3G06510 30.846 201 112 5 69 258 364 548 3.27e-17 81.3
MS.gene33495.t1 AT3G18070 43.820 89 49 1 2 89 255 343 7.40e-17 79.7
MS.gene33495.t1 AT3G06510 31.250 192 89 6 69 258 364 514 6.97e-15 74.3
MS.gene33495.t1 AT2G44470 43.333 90 49 2 2 89 259 348 8.71e-15 73.6
MS.gene33495.t1 AT1G02850 29.697 165 99 3 2 164 275 424 9.00e-15 73.9
MS.gene33495.t1 AT3G62750 35.484 93 59 1 2 93 238 330 1.69e-12 67.0
MS.gene33495.t1 AT3G62750 35.484 93 59 1 2 93 238 330 1.69e-12 67.0
MS.gene33495.t1 AT3G62750 35.484 93 59 1 2 93 264 356 2.18e-12 66.6

Find 60 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAACATAATAGAATTAT+TGG 0.142938 4.2:+85750259 MS.gene33495:CDS
GAGCAATCTAAGAGTCTTAT+TGG 0.231240 4.2:+85749171 MS.gene33495:CDS
ACTGTTAATGTGACTTTAAC+AGG 0.248704 4.2:+85749312 MS.gene33495:CDS
CAGGCTCAAAATGGTATAAT+AGG 0.277472 4.2:+85748882 None:intergenic
ACGATTATCATAACAGAAAA+TGG 0.280720 4.2:+85749636 MS.gene33495:CDS
ATACTTTGCATGGTCCCTTT+TGG 0.290939 4.2:+85750123 MS.gene33495:CDS
AGCTCTCTGCCCGGTGGTTT+AGG 0.309537 4.2:+85750230 MS.gene33495:CDS
GACGGATACACTGTGCGTTT+TGG 0.311280 4.2:+85750163 MS.gene33495:CDS
GACGCCCTTGATTTCATGTT+TGG 0.322541 4.2:+85748972 MS.gene33495:CDS
ATGTTTGTCCAAAGGGGATT+CGG 0.336791 4.2:+85749574 MS.gene33495:CDS
ACCACATATCAGGCTCAAAA+TGG 0.345183 4.2:+85748873 None:intergenic
TCTAATGGGTCAGTTGAGTA+TGG 0.349718 4.2:-85748933 None:intergenic
CATGAAATCAAGGGCGTCTT+CGG 0.355937 4.2:-85748967 None:intergenic
GCAGTGAACGTTGGTAAATC+TGG 0.366493 4.2:-85749147 None:intergenic
GATCCAGACTGTATAGGAAT+TGG 0.386737 4.2:-85749255 None:intergenic
AGGCAAGGTGTGAATGTAAA+AGG 0.387936 4.2:+85750100 MS.gene33495:intron
TGTTTGTCCAAAGGGGATTC+GGG 0.400286 4.2:+85749575 MS.gene33495:CDS
TCCATTCAAAGTTGTCCAAA+AGG 0.405927 4.2:-85750138 None:intergenic
TGGCTATATGTTTGTCCAAA+GGG 0.410402 4.2:+85749567 MS.gene33495:CDS
TTTATACAGCCGCTAACAAC+AGG 0.426920 4.2:+85749093 MS.gene33495:CDS
ACATAAGACACCAAGCTTGC+TGG 0.447607 4.2:-85749123 None:intergenic
AACCACCGGGCAGAGAGCTT+AGG 0.450563 4.2:-85750226 None:intergenic
AGTAGGTCCCGAATCCCCTT+TGG 0.457019 4.2:-85749582 None:intergenic
ATGACAGATAAGAATAAAGA+TGG 0.458312 4.2:+85749445 MS.gene33495:intron
CCGCCTGATCCAGACTGTAT+AGG 0.458956 4.2:-85749261 None:intergenic
ACCATCCAAACATGAAATCA+AGG 0.473106 4.2:-85748977 None:intergenic
ACCACCGGGCAGAGAGCTTA+GGG 0.475465 4.2:-85750225 None:intergenic
ATCACTCGAAGAGGCGCTCA+TGG 0.478154 4.2:+85749905 MS.gene33495:CDS
AATAAAGATGGGACATACAT+TGG 0.483048 4.2:+85749457 MS.gene33495:CDS
ATTCCTATACAGTCTGGATC+AGG 0.483543 4.2:+85749258 MS.gene33495:CDS
CAAAATGGTATAATAGGCAT+TGG 0.486147 4.2:+85748888 MS.gene33495:CDS
GCATTGGATTAAACTCTCAC+TGG 0.490284 4.2:+85748904 MS.gene33495:CDS
AGCAATCTAAGAGTCTTATT+GGG 0.490733 4.2:+85749172 MS.gene33495:CDS
CCATTCAAAGTTGTCCAAAA+GGG 0.499789 4.2:-85750137 None:intergenic
CCCTTGATTTCATGTTTGGA+TGG 0.504970 4.2:+85748976 MS.gene33495:CDS
TCTGGATCAGGCGGCGCTGA+CGG 0.508743 4.2:+85749270 MS.gene33495:CDS
AGGAATTGGTTTCGTTGCAT+TGG 0.510612 4.2:-85749241 None:intergenic
AAACCAATTCCTATACAGTC+TGG 0.511299 4.2:+85749252 MS.gene33495:CDS
AGGAAAGTATCCAGCAAGCT+TGG 0.526086 4.2:+85749113 MS.gene33495:CDS
GACAACTTTGAATGGAATGA+CGG 0.527539 4.2:+85750145 MS.gene33495:CDS
GGATACTTTCCTGTTGTTAG+CGG 0.535598 4.2:-85749102 None:intergenic
AATATGTGCAGGCTGCAACT+TGG 0.539391 4.2:+85749547 MS.gene33495:intron
CAAGAAACTTCCTAAACCAC+CGG 0.545200 4.2:-85750240 None:intergenic
TTGGCTATATGTTTGTCCAA+AGG 0.547740 4.2:+85749566 MS.gene33495:CDS
TTTCCTTTGTGTACAACAGT+AGG 0.554600 4.2:-85749599 None:intergenic
GACACCCTAAGCTCTCTGCC+CGG 0.573913 4.2:+85750221 MS.gene33495:CDS
TGGGACATACATTGGAGCAT+GGG 0.581493 4.2:+85749465 MS.gene33495:CDS
ATGGGACATACATTGGAGCA+TGG 0.582897 4.2:+85749464 MS.gene33495:CDS
GATTGCTCTGCAGTGAACGT+TGG 0.602380 4.2:-85749156 None:intergenic
GGCTATATGTTTGTCCAAAG+GGG 0.602874 4.2:+85749568 MS.gene33495:CDS
TGACAGATAAGAATAAAGAT+GGG 0.612129 4.2:+85749446 MS.gene33495:intron
ATGTAAAAGGATACTTTGCA+TGG 0.622027 4.2:+85750113 MS.gene33495:CDS
TCCAACACTATCACTCGAAG+AGG 0.624433 4.2:+85749896 MS.gene33495:CDS
CCATCCAAACATGAAATCAA+GGG 0.640294 4.2:-85748976 None:intergenic
GCCTCTTCGAGTGATAGTGT+TGG 0.646940 4.2:-85749897 None:intergenic
TGCATTGGCAGCATACATGC+TGG 0.650774 4.2:-85749226 None:intergenic
CCTATACAGTCTGGATCAGG+CGG 0.652705 4.2:+85749261 MS.gene33495:CDS
AAGAAACTTCCTAAACCACC+GGG 0.661886 4.2:-85750239 None:intergenic
GGACCTACTGTTGTACACAA+AGG 0.673741 4.2:+85749596 MS.gene33495:CDS
ACCCTAAGCTCTCTGCCCGG+TGG 0.683098 4.2:+85750224 MS.gene33495:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGATTTCAAATTATA+CGG - chr4.2:85749060-85749079 None:intergenic 10.0%
!! TTCTAATAAATTTAAATCAT+AGG + chr4.2:85749855-85749874 MS.gene33495:intron 10.0%
!! AAAACAATTTATGTCAAACA+AGG - chr4.2:85749413-85749432 None:intergenic 20.0%
!! ATTCTGATTTAAGTAAATAG+AGG - chr4.2:85749996-85750015 None:intergenic 20.0%
!! CTTGAACATAATAGAATTAT+TGG + chr4.2:85750259-85750278 MS.gene33495:CDS 20.0%
!! TTCTGATTTAAGTAAATAGA+GGG - chr4.2:85749995-85750014 None:intergenic 20.0%
!!! AATCTTTTTTATCTGTTTAC+AGG + chr4.2:85749070-85749089 MS.gene33495:intron 20.0%
!!! TTGACTATATTGTTGTTTTT+TGG + chr4.2:85750065-85750084 MS.gene33495:intron 20.0%
! ACGATTATCATAACAGAAAA+TGG + chr4.2:85749636-85749655 MS.gene33495:CDS 25.0%
! ATGACAGATAAGAATAAAGA+TGG + chr4.2:85749445-85749464 MS.gene33495:intron 25.0%
! GTTTATTAGACACTGAAAAA+AGG - chr4.2:85749822-85749841 None:intergenic 25.0%
! TGACAGATAAGAATAAAGAT+GGG + chr4.2:85749446-85749465 MS.gene33495:intron 25.0%
!! ATTATCTTTTATCTGCAATG+AGG + chr4.2:85749964-85749983 MS.gene33495:CDS 25.0%
!! ATTGGGTCATATGATTTTAT+TGG + chr4.2:85749189-85749208 MS.gene33495:CDS 25.0%
!! GTTTGACATAAATTGTTTTC+TGG + chr4.2:85749414-85749433 MS.gene33495:intron 25.0%
!! TTTTCTGTTATGATAATCGT+AGG - chr4.2:85749636-85749655 None:intergenic 25.0%
!!! TTTTTTGGTTTTTTGTGTGA+AGG + chr4.2:85750080-85750099 MS.gene33495:intron 25.0%
AACAACTAAACAATATGTGC+AGG + chr4.2:85749536-85749555 MS.gene33495:intron 30.0%
AATAAAGATGGGACATACAT+TGG + chr4.2:85749457-85749476 MS.gene33495:CDS 30.0%
ACTGTTAATGTGACTTTAAC+AGG + chr4.2:85749312-85749331 MS.gene33495:CDS 30.0%
ATGTAAAAGGATACTTTGCA+TGG + chr4.2:85750113-85750132 MS.gene33495:CDS 30.0%
CAAAATGGTATAATAGGCAT+TGG + chr4.2:85748888-85748907 MS.gene33495:CDS 30.0%
!! AGCAATCTAAGAGTCTTATT+GGG + chr4.2:85749172-85749191 MS.gene33495:CDS 30.0%
AAACCAATTCCTATACAGTC+TGG + chr4.2:85749252-85749271 MS.gene33495:CDS 35.0%
ACCATCCAAACATGAAATCA+AGG - chr4.2:85748980-85748999 None:intergenic 35.0%
ATCCATGGTTGTTGTAGAAA+CGG - chr4.2:85749021-85749040 None:intergenic 35.0%
CCATCCAAACATGAAATCAA+GGG - chr4.2:85748979-85748998 None:intergenic 35.0%
CCATTCAAAGTTGTCCAAAA+GGG - chr4.2:85750140-85750159 None:intergenic 35.0%
GACAACTTTGAATGGAATGA+CGG + chr4.2:85750145-85750164 MS.gene33495:CDS 35.0%
TCCATTCAAAGTTGTCCAAA+AGG - chr4.2:85750141-85750160 None:intergenic 35.0%
TGATGTATGCCATCTTCATT+AGG - chr4.2:85749360-85749379 None:intergenic 35.0%
TGGCTATATGTTTGTCCAAA+GGG + chr4.2:85749567-85749586 MS.gene33495:CDS 35.0%
TTCATTAGGAGTGGTAAGAA+AGG - chr4.2:85749346-85749365 None:intergenic 35.0%
TTGGCTATATGTTTGTCCAA+AGG + chr4.2:85749566-85749585 MS.gene33495:CDS 35.0%
TTTCCTTTGTGTACAACAGT+AGG - chr4.2:85749602-85749621 None:intergenic 35.0%
! GAAACGGTCAAAAAGTATAG+TGG - chr4.2:85749005-85749024 None:intergenic 35.0%
! GAGCAATCTAAGAGTCTTAT+TGG + chr4.2:85749171-85749190 MS.gene33495:CDS 35.0%
AAGAAACTTCCTAAACCACC+GGG - chr4.2:85750242-85750261 None:intergenic 40.0%
ACCATGGATCACGACAAAAT+TGG + chr4.2:85749032-85749051 MS.gene33495:intron 40.0%
AGGCAAGGTGTGAATGTAAA+AGG + chr4.2:85750100-85750119 MS.gene33495:intron 40.0%
ATGTTTGTCCAAAGGGGATT+CGG + chr4.2:85749574-85749593 MS.gene33495:CDS 40.0%
ATTCCTATACAGTCTGGATC+AGG + chr4.2:85749258-85749277 MS.gene33495:CDS 40.0%
CAAGAAACTTCCTAAACCAC+CGG - chr4.2:85750243-85750262 None:intergenic 40.0%
CCATGGATCACGACAAAATT+GGG + chr4.2:85749033-85749052 MS.gene33495:intron 40.0%
CCCTTGATTTCATGTTTGGA+TGG + chr4.2:85748976-85748995 MS.gene33495:CDS 40.0%
CTTACCACTCCTAATGAAGA+TGG + chr4.2:85749348-85749367 MS.gene33495:intron 40.0%
GATCCAGACTGTATAGGAAT+TGG - chr4.2:85749258-85749277 None:intergenic 40.0%
GCATTGGATTAAACTCTCAC+TGG + chr4.2:85748904-85748923 MS.gene33495:CDS 40.0%
GGATACTTTCCTGTTGTTAG+CGG - chr4.2:85749105-85749124 None:intergenic 40.0%
GGCTATATGTTTGTCCAAAG+GGG + chr4.2:85749568-85749587 MS.gene33495:CDS 40.0%
TATGCCATCTTCATTAGGAG+TGG - chr4.2:85749355-85749374 None:intergenic 40.0%
TTTATACAGCCGCTAACAAC+AGG + chr4.2:85749093-85749112 MS.gene33495:CDS 40.0%
! AGGAATTGGTTTCGTTGCAT+TGG - chr4.2:85749244-85749263 None:intergenic 40.0%
! ATACTTTGCATGGTCCCTTT+TGG + chr4.2:85750123-85750142 MS.gene33495:CDS 40.0%
! CCCTTTTGGACAACTTTGAA+TGG + chr4.2:85750137-85750156 MS.gene33495:CDS 40.0%
! CGGCAGCTTTTAGATCTAAT+GGG - chr4.2:85748950-85748969 None:intergenic 40.0%
! TCGGCAGCTTTTAGATCTAA+TGG - chr4.2:85748951-85748970 None:intergenic 40.0%
! TCTAATGGGTCAGTTGAGTA+TGG - chr4.2:85748936-85748955 None:intergenic 40.0%
!!! TGGTTTTTTGTGTGAAGGCA+AGG + chr4.2:85750085-85750104 MS.gene33495:intron 40.0%
AATATGTGCAGGCTGCAACT+TGG + chr4.2:85749547-85749566 MS.gene33495:intron 45.0%
ACATAAGACACCAAGCTTGC+TGG - chr4.2:85749126-85749145 None:intergenic 45.0%
ATGGGACATACATTGGAGCA+TGG + chr4.2:85749464-85749483 MS.gene33495:CDS 45.0%
GACCGTTTCTACAACAACCA+TGG + chr4.2:85749016-85749035 MS.gene33495:intron 45.0%
GACGCCCTTGATTTCATGTT+TGG + chr4.2:85748972-85748991 MS.gene33495:CDS 45.0%
GGACCTACTGTTGTACACAA+AGG + chr4.2:85749596-85749615 MS.gene33495:CDS 45.0%
TCCAACACTATCACTCGAAG+AGG + chr4.2:85749896-85749915 MS.gene33495:CDS 45.0%
TGGGACATACATTGGAGCAT+GGG + chr4.2:85749465-85749484 MS.gene33495:CDS 45.0%
TGTTTGTCCAAAGGGGATTC+GGG + chr4.2:85749575-85749594 MS.gene33495:CDS 45.0%
! CCCAATTTTGTCGTGATCCA+TGG - chr4.2:85749036-85749055 None:intergenic 45.0%
! GCAGTGAACGTTGGTAAATC+TGG - chr4.2:85749150-85749169 None:intergenic 45.0%
!! AGGAAAGTATCCAGCAAGCT+TGG + chr4.2:85749113-85749132 MS.gene33495:CDS 45.0%
!! CATGAAATCAAGGGCGTCTT+CGG - chr4.2:85748970-85748989 None:intergenic 45.0%
CCTATACAGTCTGGATCAGG+CGG + chr4.2:85749261-85749280 MS.gene33495:CDS 50.0%
GATTGCTCTGCAGTGAACGT+TGG - chr4.2:85749159-85749178 None:intergenic 50.0%
! GACGGATACACTGTGCGTTT+TGG + chr4.2:85750163-85750182 MS.gene33495:CDS 50.0%
! GCCTCTTCGAGTGATAGTGT+TGG - chr4.2:85749900-85749919 None:intergenic 50.0%
! TGCATTGGCAGCATACATGC+TGG - chr4.2:85749229-85749248 None:intergenic 50.0%
AGTAGGTCCCGAATCCCCTT+TGG - chr4.2:85749585-85749604 None:intergenic 55.0%
CCGCCTGATCCAGACTGTAT+AGG - chr4.2:85749264-85749283 None:intergenic 55.0%
!! ATCACTCGAAGAGGCGCTCA+TGG + chr4.2:85749905-85749924 MS.gene33495:CDS 55.0%
AACCACCGGGCAGAGAGCTT+AGG - chr4.2:85750229-85750248 None:intergenic 60.0%
ACCACCGGGCAGAGAGCTTA+GGG - chr4.2:85750228-85750247 None:intergenic 60.0%
AGCTCTCTGCCCGGTGGTTT+AGG + chr4.2:85750230-85750249 MS.gene33495:CDS 60.0%
GACACCCTAAGCTCTCTGCC+CGG + chr4.2:85750221-85750240 MS.gene33495:CDS 60.0%
ACCCTAAGCTCTCTGCCCGG+TGG + chr4.2:85750224-85750243 MS.gene33495:CDS 65.0%
! TCTGGATCAGGCGGCGCTGA+CGG + chr4.2:85749270-85749289 MS.gene33495:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 85748885 85750285 85748885 ID=MS.gene33495
chr4.2 mRNA 85748885 85750285 85748885 ID=MS.gene33495.t1;Parent=MS.gene33495
chr4.2 exon 85748885 85748997 85748885 ID=MS.gene33495.t1.exon1;Parent=MS.gene33495.t1
chr4.2 CDS 85748885 85748997 85748885 ID=cds.MS.gene33495.t1;Parent=MS.gene33495.t1
chr4.2 exon 85749092 85749333 85749092 ID=MS.gene33495.t1.exon2;Parent=MS.gene33495.t1
chr4.2 CDS 85749092 85749333 85749092 ID=cds.MS.gene33495.t1;Parent=MS.gene33495.t1
chr4.2 exon 85749452 85749486 85749452 ID=MS.gene33495.t1.exon3;Parent=MS.gene33495.t1
chr4.2 CDS 85749452 85749486 85749452 ID=cds.MS.gene33495.t1;Parent=MS.gene33495.t1
chr4.2 exon 85749558 85749657 85749558 ID=MS.gene33495.t1.exon4;Parent=MS.gene33495.t1
chr4.2 CDS 85749558 85749657 85749558 ID=cds.MS.gene33495.t1;Parent=MS.gene33495.t1
chr4.2 exon 85749877 85749985 85749877 ID=MS.gene33495.t1.exon5;Parent=MS.gene33495.t1
chr4.2 CDS 85749877 85749985 85749877 ID=cds.MS.gene33495.t1;Parent=MS.gene33495.t1
chr4.2 exon 85750102 85750285 85750102 ID=MS.gene33495.t1.exon6;Parent=MS.gene33495.t1
chr4.2 CDS 85750102 85750285 85750102 ID=cds.MS.gene33495.t1;Parent=MS.gene33495.t1
Gene Sequence

>MS.gene33495

GCTCAAAATGGTATAATAGGCATTGGATTAAACTCTCACTGGTTTAAACCATACTCAACTGACCCATTAGATCTAAAAGCTGCCGAAGACGCCCTTGATTTCATGTTTGGATGGTAACCACTATACTTTTTGACCGTTTCTACAACAACCATGGATCACGACAAAATTGGGACCGTATAATTTGAAATCTTTTTTATCTGTTTACAGGTTTATACAGCCGCTAACAACAGGAAAGTATCCAGCAAGCTTGGTGTCTTATGTGCCAGATTTACCAACGTTCACTGCAGAGCAATCTAAGAGTCTTATTGGGTCATATGATTTTATTGGAATCAATTACTACACCAGCATGTATGCTGCCAATGCAACGAAACCAATTCCTATACAGTCTGGATCAGGCGGCGCTGACGGTGTCAATAGTGTTTTCAATACTGTTAATGTGACTTTAACAGGTAAGAACACCTTTCTTACCACTCCTAATGAAGATGGCATACATCATTAATAGAAAAATCAATGTGTGCTATGATTCCTTGTTTGACATAAATTGTTTTCTGGTATGATTCATGACAGATAAGAATAAAGATGGGACATACATTGGAGCATGGGTGAGTAAGACTCTTAAGATTATTGTTACATCTTATTTGATTGATGAATAACAACTAAACAATATGTGCAGGCTGCAACTTGGCTATATGTTTGTCCAAAGGGGATTCGGGACCTACTGTTGTACACAAAGGAAAAGTATAACAATCCTACGATTATCATAACAGAAAATGGTAATATTTATAATACATTCTTTTATTTTTTTTATTTACTTCTAACATAAAATTTAAGAAAATATATTATGTATATATCAATCATTTTATATATTAAATCTACTAATTGTCATTAAAAAAAGCAAATACTTCTAATATTCTCTCACAGTGTATTTCATTCTCCTTTTTTCAGTGTCTAATAAACTAAAATTTGAAAATTCTAATAAATTTAAATCATAGGTATGAATGAAGTCAATGATCCAACACTATCACTCGAAGAGGCGCTCATGGATACTAACAGAATTGATTACTTTTATCGTCATCTATATTATCTTTTATCTGCAATGAGGTAGCACCCTCTATTTACTTAAATCAGAATCAATTATATTATTTTCGCACTCTAATTTTTTAGTTTTATGCATTTAATTTTGACTATATTGTTGTTTTTTGGTTTTTTGTGTGAAGGCAAGGTGTGAATGTAAAAGGATACTTTGCATGGTCCCTTTTGGACAACTTTGAATGGAATGACGGATACACTGTGCGTTTTGGAATTAACTTTGTTGATTATGACAATGATCTTAGAAGACACCCTAAGCTCTCTGCCCGGTGGTTTAGGAAGTTTCTTGAACATAATAGAATTATTGGTTGA
Protein sequence

>MS.gene33495.t1

AQNGIIGIGLNSHWFKPYSTDPLDLKAAEDALDFMFGWFIQPLTTGKYPASLVSYVPDLPTFTAEQSKSLIGSYDFIGINYYTSMYAANATKPIPIQSGSGGADGVNSVFNTVNVTLTDKNKDGTYIGAWAATWLYVCPKGIRDLLLYTKEKYNNPTIIITENGMNEVNDPTLSLEEALMDTNRIDYFYRHLYYLLSAMRQGVNVKGYFAWSLLDNFEWNDGYTVRFGINFVDYDNDLRRHPKLSARWFRKFLEHNRIIG