AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34135

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34135.t1 MTR_1g100240 96.703 364 10 1 1 364 1 362 0.0 723
MS.gene34135.t1 MTR_5g089950 30.688 189 122 4 61 244 6 190 1.16e-19 88.6
MS.gene34135.t1 MTR_8g062340 30.108 186 127 2 61 244 6 190 1.57e-19 88.2
MS.gene34135.t1 MTR_3g080040 29.255 188 128 3 61 245 6 191 1.91e-19 87.4
MS.gene34135.t1 MTR_8g062340 30.108 186 127 2 61 244 6 190 8.78e-19 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34135.t1 AT4G21800 74.174 333 85 1 19 351 4 335 0.0 523
MS.gene34135.t1 AT4G21800 74.174 333 85 1 19 351 4 335 0.0 523
MS.gene34135.t1 AT4G12790 30.857 175 118 3 61 233 6 179 1.04e-19 88.2
MS.gene34135.t1 AT4G12790 30.857 175 118 3 61 233 6 179 1.04e-19 88.2
MS.gene34135.t1 AT4G12790 30.857 175 118 3 61 233 6 179 1.04e-19 88.2
MS.gene34135.t1 AT4G12790 30.857 175 118 3 61 233 6 179 1.04e-19 88.2
MS.gene34135.t1 AT4G12790 30.857 175 118 3 61 233 6 179 1.04e-19 88.2
MS.gene34135.t1 AT4G12790 30.857 175 118 3 61 233 6 179 1.04e-19 88.2
MS.gene34135.t1 AT5G22370 27.957 186 131 3 61 244 6 190 1.19e-15 77.0
MS.gene34135.t1 AT5G22370 27.957 186 131 3 61 244 6 190 1.19e-15 77.0
MS.gene34135.t1 AT5G22370 27.957 186 131 3 61 244 6 190 1.19e-15 77.0

Find 77 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTGAAGGTTCCTCTTC+TGG 0.208586 1.2:-75031279 MS.gene34135:CDS
TACTCATCTGTCAAACATTA+GGG 0.298804 1.2:-75030973 MS.gene34135:CDS
ATACTCATCTGTCAAACATT+AGG 0.301640 1.2:-75030974 MS.gene34135:CDS
CCTGTTATCATCATTGTTGT+TGG 0.328714 1.2:-75031234 MS.gene34135:CDS
CCAACAACAATGATGATAAC+AGG 0.357092 1.2:+75031234 None:intergenic
ATATTCACATGGTCTGCTTC+TGG 0.363085 1.2:-75030300 MS.gene34135:CDS
GCTGGAGTTTCTGCAGTTAC+CGG 0.366983 1.2:-75029133 MS.gene34135:CDS
GAGTGAGTCTATGAAGAAAT+TGG 0.370198 1.2:-75031301 MS.gene34135:CDS
GAAGTCAAAATTCCACCATT+GGG 0.397466 1.2:+75030840 None:intergenic
GTTTCTGCAGTTACCGGTGC+TGG 0.411468 1.2:-75029127 MS.gene34135:CDS
TACAGTAATTTAAGATCAGC+TGG 0.416370 1.2:-75029151 MS.gene34135:CDS
ATCATTGTTGTTGGAATGGC+AGG 0.426570 1.2:-75031225 MS.gene34135:intron
TTCCATGTATTCCTCTGCAC+TGG 0.431616 1.2:+75029075 None:intergenic
TTTAACTCAGAGCCTTTCTC+TGG 0.457020 1.2:-75029188 MS.gene34135:CDS
AAACTTAACCTCATCGAACT+TGG 0.460043 1.2:+75030800 None:intergenic
GATCAGAACTTGCAGCTGCT+TGG 0.463589 1.2:+75029227 None:intergenic
ATGAAGAAATTGGACATTGA+AGG 0.465533 1.2:-75031291 MS.gene34135:CDS
ACAACATGAGTTTGCCTTGG+AGG 0.468476 1.2:-75030103 MS.gene34135:intron
AAATTCCACCATTGGGGCCA+AGG 0.471975 1.2:+75030847 None:intergenic
AGGAGGGAGATGGAGAAATC+TGG 0.473893 1.2:-75028840 MS.gene34135:CDS
GTCACCGGCTTCCATTTCCA+TGG 0.485145 1.2:+75031572 None:intergenic
CTCATCTGTGTTTAGGGAGT+GGG 0.487079 1.2:-75031028 MS.gene34135:intron
TCACCGGCTTCCATTTCCAT+GGG 0.489818 1.2:+75031573 None:intergenic
ATATCAATGTTAGAAGCATA+TGG 0.489870 1.2:+75030912 None:intergenic
CAATTCAACCTTGGCCCCAA+TGG 0.493147 1.2:-75030855 MS.gene34135:CDS
AGAAGTCAAAATTCCACCAT+TGG 0.493187 1.2:+75030839 None:intergenic
TCAGAGCCTTTCTCTGGCAC+TGG 0.495072 1.2:-75029182 MS.gene34135:CDS
AGCACAACATGAGTTTGCCT+TGG 0.507905 1.2:-75030106 MS.gene34135:CDS
AAGCATGTTGCTCATAAAAG+TGG 0.519169 1.2:+75030194 None:intergenic
AGGAGAACATGGATAAACTT+AGG 0.522057 1.2:-75028860 MS.gene34135:CDS
TATGGAAGTGTCATGACAGC+CGG 0.522098 1.2:+75030930 None:intergenic
TCAACTTGACTATGTTCTTG+TGG 0.522799 1.2:-75030343 MS.gene34135:CDS
ATTCATCCAGTGCCAGAGAA+AGG 0.526358 1.2:+75029176 None:intergenic
GAAGCCTTCGATTCCAGCAC+CGG 0.536218 1.2:+75029114 None:intergenic
TTCAACCTTGGCCCCAATGG+TGG 0.539374 1.2:-75030852 MS.gene34135:CDS
AGCCAGTGCAGAGGAATACA+TGG 0.540021 1.2:-75029077 MS.gene34135:CDS
TATGTTCTTGTGGATACTCC+TGG 0.544128 1.2:-75030333 MS.gene34135:CDS
GAAGTTAGGTGAACCAGAAG+AGG 0.546699 1.2:+75031266 None:intergenic
CACCCTCCGCAACATGGACG+CGG 0.548806 1.2:-75031631 None:intergenic
TATCATCATTGTTGTTGGAA+TGG 0.555919 1.2:-75031229 MS.gene34135:CDS
GGATAAACTTAGGAGGGAGA+TGG 0.557760 1.2:-75028850 MS.gene34135:CDS
AGAAAGTACTTGAGAGTCAC+CGG 0.565078 1.2:+75031557 None:intergenic
GACTACACTCATGCATAGGT+TGG 0.567808 1.2:-75031003 MS.gene34135:CDS
GAAACTGTAGTGTTGAGCAC+TGG 0.570041 1.2:-75028813 MS.gene34135:intron
AGCTGTTGGAAGAAAATCGC+AGG 0.575043 1.2:-75028884 MS.gene34135:CDS
AAATCGCAGGAAGGAGAACA+TGG 0.576913 1.2:-75028871 MS.gene34135:CDS
GTTACCGGTGCTGGAATCGA+AGG 0.577784 1.2:-75029118 MS.gene34135:CDS
ATGGAAGTGTCATGACAGCC+GGG 0.578869 1.2:+75030931 None:intergenic
GGCAACTACAGTGGGAAATG+TGG 0.579709 1.2:+75030251 None:intergenic
ATTTCTTCATAGACTCACTC+AGG 0.587164 1.2:+75031304 None:intergenic
GTGAATATCTCAATCTGACC+AGG 0.587935 1.2:+75030315 None:intergenic
AGAACATGGATAAACTTAGG+AGG 0.588077 1.2:-75028857 MS.gene34135:CDS
GTCATGAAGCAATTCAACCT+TGG 0.600059 1.2:-75030864 MS.gene34135:CDS
AGGGAGATGGAGAAATCTGG+AGG 0.604309 1.2:-75028837 MS.gene34135:CDS
ACTACATAGGCAACTACAGT+GGG 0.605400 1.2:+75030243 None:intergenic
ACGAGGTGTATCAACTACAT+AGG 0.606135 1.2:+75030230 None:intergenic
ACGTGAAGAGAAACAGCTGT+TGG 0.606507 1.2:-75028898 MS.gene34135:CDS
GAACATGGATAAACTTAGGA+GGG 0.606999 1.2:-75028856 MS.gene34135:CDS
TTATGTGATGAATCTCGACC+CGG 0.607451 1.2:-75030949 MS.gene34135:CDS
GAGGTAACCTTGTCTTATAG+AGG 0.608238 1.2:+75030157 None:intergenic
CAAAATCAACAATACCTCCA+AGG 0.609268 1.2:+75030089 None:intergenic
TTGTTGAATGCTAAGATGAG+AGG 0.620285 1.2:+75030138 None:intergenic
AACTACAGTGGGAAATGTGG+AGG 0.621918 1.2:+75030254 None:intergenic
ATGTTGCTCATAAAAGTGGT+AGG 0.622477 1.2:+75030198 None:intergenic
AGCTGTTGAAGCCAGTGCAG+AGG 0.624332 1.2:-75029086 MS.gene34135:CDS
CACCGGCTTCCATTTCCATG+GGG 0.630823 1.2:+75031574 None:intergenic
GGAAGACTACACTCATGCAT+AGG 0.648368 1.2:-75031007 MS.gene34135:CDS
ACCGGCTTCCATTTCCATGG+GGG 0.657686 1.2:+75031575 None:intergenic
GTTTGCAACCAAGTTCGATG+AGG 0.658878 1.2:-75030808 MS.gene34135:intron
ACTAGTAGGAGTAATTGCAA+AGG 0.660440 1.2:-75028472 MS.gene34135:intron
GTTGGAAGAAAATCGCAGGA+AGG 0.666047 1.2:-75028880 MS.gene34135:CDS
AAGTCAAAATTCCACCATTG+GGG 0.670613 1.2:+75030841 None:intergenic
AACTACATAGGCAACTACAG+TGG 0.686317 1.2:+75030242 None:intergenic
GTAGTATCCTCTATAAGACA+AGG 0.691493 1.2:-75030164 MS.gene34135:CDS
ACTCATCTGTCAAACATTAG+GGG 0.701476 1.2:-75030972 MS.gene34135:CDS
TCTCATCTTAGCATTCAACA+AGG 0.708282 1.2:-75030136 MS.gene34135:CDS
GTCAGATTGAGATATTCACA+TGG 0.740453 1.2:-75030311 MS.gene34135:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAAACAAAATTTAATT+AGG - chr1.2:75028439-75028458 MS.gene34135:intron 10.0%
!! ACATATAAATACTTATTAAA+AGG - chr1.2:75030283-75030302 MS.gene34135:CDS 10.0%
!!! TGTTTAAGTTATTATTTTAT+AGG - chr1.2:75030447-75030466 MS.gene34135:intron 10.0%
!! AATAATAACTTAAACATGAA+AGG + chr1.2:75030443-75030462 None:intergenic 15.0%
!! ATAAATAAATCATGACATTT+GGG - chr1.2:75030091-75030110 MS.gene34135:intron 15.0%
!! ATAATAACTTAAACATGAAA+GGG + chr1.2:75030442-75030461 None:intergenic 15.0%
!! CATGAAAAAAAATAAATTGT+TGG + chr1.2:75029472-75029491 None:intergenic 15.0%
!!! AATACAATAAAAACGTTTTT+AGG + chr1.2:75028533-75028552 None:intergenic 15.0%
!!! ATACAATAAAAACGTTTTTA+GGG + chr1.2:75028532-75028551 None:intergenic 15.0%
!!! ATTTTAACATTTCATTCAAT+TGG - chr1.2:75030321-75030340 MS.gene34135:CDS 15.0%
!! AAAACAGATACTAGTTATAT+GGG + chr1.2:75031552-75031571 None:intergenic 20.0%
!! ATAGAATGTATGAAATAACA+GGG + chr1.2:75031406-75031425 None:intergenic 20.0%
!! CAAAATCAAAAGTGAAATAA+TGG + chr1.2:75030939-75030958 None:intergenic 20.0%
!! CAGTATAATATATATATTGC+TGG - chr1.2:75029188-75029207 MS.gene34135:CDS 20.0%
!! GATAAATAAATCATGACATT+TGG - chr1.2:75030090-75030109 MS.gene34135:intron 20.0%
!! GCATAAAAAAAAATTAAGCA+GGG + chr1.2:75029309-75029328 None:intergenic 20.0%
!! GTTCAAATTAAGTACTTAAA+AGG + chr1.2:75030060-75030079 None:intergenic 20.0%
!! TAAAATATACTTTGCTTGTT+TGG + chr1.2:75028836-75028855 None:intergenic 20.0%
!! TACTAGTATAAAAAACATCT+AGG + chr1.2:75031455-75031474 None:intergenic 20.0%
!! TATAGTATACAATACTAACA+CGG + chr1.2:75028866-75028885 None:intergenic 20.0%
!!! AACTTTTAAGTTTAACAGAT+TGG - chr1.2:75030376-75030395 MS.gene34135:intron 20.0%
!!! AATATATATATTGCTGGTTA+TGG - chr1.2:75029194-75029213 MS.gene34135:CDS 20.0%
!!! ACTTTTAAGTTTAACAGATT+GGG - chr1.2:75030377-75030396 MS.gene34135:intron 20.0%
!!! GTTTTACTTATATATGCATT+CGG - chr1.2:75029238-75029257 MS.gene34135:CDS 20.0%
!!! TAATTTGAAATTGTGAATCA+TGG - chr1.2:75028571-75028590 MS.gene34135:intron 20.0%
!!! TAGATGTTTTTTATACTAGT+AGG - chr1.2:75031454-75031473 MS.gene34135:intron 20.0%
!!! TATCATTTTTATAGCTTTCA+AGG + chr1.2:75029927-75029946 None:intergenic 20.0%
!!! TCTAATTGATAGTTTTATCT+CGG - chr1.2:75030006-75030025 MS.gene34135:intron 20.0%
!!! TGCTTAATTTTTTTTTATGC+CGG - chr1.2:75029309-75029328 MS.gene34135:intron 20.0%
!!! TTTTGATTTTGCATTTAGTT+AGG - chr1.2:75030948-75030967 MS.gene34135:CDS 20.0%
! AAGTCTAACTCTTAATTCAT+AGG + chr1.2:75030199-75030218 None:intergenic 25.0%
! AATATAACTCATCTGTGTTT+AGG - chr1.2:75028905-75028924 MS.gene34135:CDS 25.0%
! ATAGTATACAATACTAACAC+GGG + chr1.2:75028865-75028884 None:intergenic 25.0%
! ATATAACTCATCTGTGTTTA+GGG - chr1.2:75028906-75028925 MS.gene34135:CDS 25.0%
! ATTATACTGTACATGAGTTT+AGG + chr1.2:75029177-75029196 None:intergenic 25.0%
! CAAAACAGATACTAGTTATA+TGG + chr1.2:75031553-75031572 None:intergenic 25.0%
! GATAGAATGTATGAAATAAC+AGG + chr1.2:75031407-75031426 None:intergenic 25.0%
! GGAAAAAAACAAACAAAAAG+AGG - chr1.2:75028612-75028631 MS.gene34135:intron 25.0%
! GGCATAAAAAAAAATTAAGC+AGG + chr1.2:75029310-75029329 None:intergenic 25.0%
! TATACCAATAGCTTAATTAG+AGG + chr1.2:75029378-75029397 None:intergenic 25.0%
! TTTACTTCATATTGCATGTT+TGG - chr1.2:75028758-75028777 MS.gene34135:intron 25.0%
! TTTCTATTATGCGATGTTTA+GGG - chr1.2:75031003-75031022 MS.gene34135:CDS 25.0%
!! ATATCAATGTTAGAAGCATA+TGG + chr1.2:75029031-75029050 None:intergenic 25.0%
!! TTTTCTATTATGCGATGTTT+AGG - chr1.2:75031002-75031021 MS.gene34135:CDS 25.0%
!!! AACTAGTATCTGTTTTGTTT+AGG - chr1.2:75031556-75031575 MS.gene34135:intron 25.0%
!!! AACTTGTTTTGTTTATCTAG+TGG - chr1.2:75030670-75030689 MS.gene34135:intron 25.0%
!!! ACTAGTATCTGTTTTGTTTA+GGG - chr1.2:75031557-75031576 MS.gene34135:intron 25.0%
!!! CTTTTAAGTTTAACAGATTG+GGG - chr1.2:75030378-75030397 MS.gene34135:intron 25.0%
!!! GCTTAATTTTTTTTTATGCC+GGG - chr1.2:75029310-75029329 MS.gene34135:intron 25.0%
AACAAAACAAGTTCGACAAA+AGG + chr1.2:75030663-75030682 None:intergenic 30.0%
AACTATCAATTAGATCTCAC+AGG + chr1.2:75030000-75030019 None:intergenic 30.0%
ACAGAAATTACCTGAAATTG+AGG + chr1.2:75029559-75029578 None:intergenic 30.0%
ATACTCATCTGTCAAACATT+AGG - chr1.2:75028966-75028985 MS.gene34135:intron 30.0%
ATGAAGAAATTGGACATTGA+AGG - chr1.2:75028649-75028668 MS.gene34135:intron 30.0%
ATTTGCACAAAAATCAAGCA+TGG + chr1.2:75031497-75031516 None:intergenic 30.0%
CATAAGATCAAACAAACTCA+AGG + chr1.2:75029343-75029362 None:intergenic 30.0%
CTAGTATCAATGATATCAAG+AGG + chr1.2:75029276-75029295 None:intergenic 30.0%
GAAATAACAGGGCAATAAAT+CGG + chr1.2:75031395-75031414 None:intergenic 30.0%
GATTCAGCTCAAATATACAA+AGG + chr1.2:75030516-75030535 None:intergenic 30.0%
GGAACAAAAGTAAAACACAT+CGG + chr1.2:75029495-75029514 None:intergenic 30.0%
GTAAAGTGAAAGTGAATAAG+CGG + chr1.2:75028418-75028437 None:intergenic 30.0%
GTTCATAAAATCACATCAGA+AGG + chr1.2:75031287-75031306 None:intergenic 30.0%
TACTCATCTGTCAAACATTA+GGG - chr1.2:75028967-75028986 MS.gene34135:intron 30.0%
TGAAATTGTGAATCATGGAA+TGG - chr1.2:75028576-75028595 MS.gene34135:intron 30.0%
TGCATATAAGTTCTGTAGAT+GGG + chr1.2:75029516-75029535 None:intergenic 30.0%
TTACTTATATATGCATTCGG+TGG - chr1.2:75029241-75029260 MS.gene34135:CDS 30.0%
TTATATGTGCAATTAGAGAG+AGG + chr1.2:75030271-75030290 None:intergenic 30.0%
TTGCATATAAGTTCTGTAGA+TGG + chr1.2:75029517-75029536 None:intergenic 30.0%
TTGTCCTCTAATTAAGCTAT+TGG - chr1.2:75029371-75029390 MS.gene34135:intron 30.0%
! CTTGATTTTTGTGCAAATTG+AGG - chr1.2:75031499-75031518 MS.gene34135:intron 30.0%
! GATGTGATTTTATGAACGTT+TGG - chr1.2:75031290-75031309 MS.gene34135:CDS 30.0%
! TACAGTAATTTAAGATCAGC+TGG - chr1.2:75030789-75030808 MS.gene34135:intron 30.0%
! TATATGTAGTTTTCCTGACT+TGG - chr1.2:75028882-75028901 MS.gene34135:CDS 30.0%
! TATCATCATTGTTGTTGGAA+TGG - chr1.2:75028711-75028730 MS.gene34135:intron 30.0%
!! ATGATGATGATGTTGATTTC+GGG - chr1.2:75031200-75031219 MS.gene34135:intron 30.0%
!! GATGATGATGATGTTGATTT+CGG - chr1.2:75031199-75031218 MS.gene34135:intron 30.0%
!!! ACAGGTTTTCTTTTGAAGTT+AGG + chr1.2:75028691-75028710 None:intergenic 30.0%
!!! TGGAATGGTTTTTGTTTTCA+AGG - chr1.2:75028591-75028610 MS.gene34135:intron 30.0%
AAACAAGTTCGACAAAAGGA+AGG + chr1.2:75030659-75030678 None:intergenic 35.0%
AAACTTAACCTCATCGAACT+TGG + chr1.2:75029143-75029162 None:intergenic 35.0%
AAGCATGTTGCTCATAAAAG+TGG + chr1.2:75029749-75029768 None:intergenic 35.0%
AAGTCAAAATTCCACCATTG+GGG + chr1.2:75029102-75029121 None:intergenic 35.0%
ACTAGTAGGAGTAATTGCAA+AGG - chr1.2:75031468-75031487 MS.gene34135:intron 35.0%
ACTCATCTGTCAAACATTAG+GGG - chr1.2:75028968-75028987 MS.gene34135:intron 35.0%
AGAACATGGATAAACTTAGG+AGG - chr1.2:75031083-75031102 MS.gene34135:intron 35.0%
AGAAGTCAAAATTCCACCAT+TGG + chr1.2:75029104-75029123 None:intergenic 35.0%
AGGAGAACATGGATAAACTT+AGG - chr1.2:75031080-75031099 MS.gene34135:intron 35.0%
ATGTTGCTCATAAAAGTGGT+AGG + chr1.2:75029745-75029764 None:intergenic 35.0%
ATTTCTTCATAGACTCACTC+AGG + chr1.2:75028639-75028658 None:intergenic 35.0%
CAAAATCAACAATACCTCCA+AGG + chr1.2:75029854-75029873 None:intergenic 35.0%
CAAGAGCAGTTAATACTTGT+AGG + chr1.2:75030892-75030911 None:intergenic 35.0%
CCAACAACAATGATGATAAC+AGG + chr1.2:75028709-75028728 None:intergenic 35.0%
CCTGTTATCATCATTGTTGT+TGG - chr1.2:75028706-75028725 MS.gene34135:intron 35.0%
CTATCAGCATTATTCTCTCT+AGG - chr1.2:75031422-75031441 MS.gene34135:intron 35.0%
GAAAACAGCTATCAAGTTCA+TGG - chr1.2:75030162-75030181 MS.gene34135:CDS 35.0%
GAACATGGATAAACTTAGGA+GGG - chr1.2:75031084-75031103 MS.gene34135:intron 35.0%
GAAGTCAAAATTCCACCATT+GGG + chr1.2:75029103-75029122 None:intergenic 35.0%
GAGTGAGTCTATGAAGAAAT+TGG - chr1.2:75028639-75028658 MS.gene34135:intron 35.0%
GATGAGTTATATTCCAAGTC+AGG + chr1.2:75028898-75028917 None:intergenic 35.0%
GCTCAAATATACAAAGGAAG+AGG + chr1.2:75030510-75030529 None:intergenic 35.0%
GGCAAGAAAATATGCTTAAG+TGG + chr1.2:75030250-75030269 None:intergenic 35.0%
GTAGTATCCTCTATAAGACA+AGG - chr1.2:75029776-75029795 MS.gene34135:intron 35.0%
GTCAGATTGAGATATTCACA+TGG - chr1.2:75029629-75029648 MS.gene34135:intron 35.0%
GTTTACATTGTCATTGTCAG+TGG - chr1.2:75029415-75029434 MS.gene34135:intron 35.0%
TATAGGACCTTGACTTCATT+TGG - chr1.2:75031335-75031354 MS.gene34135:intron 35.0%
TATGAACGTTTGGATTGCAT+CGG - chr1.2:75031300-75031319 MS.gene34135:CDS 35.0%
TCAACTTGACTATGTTCTTG+TGG - chr1.2:75029597-75029616 MS.gene34135:intron 35.0%
TCTCATCTTAGCATTCAACA+AGG - chr1.2:75029804-75029823 MS.gene34135:intron 35.0%
TCTGTTCAACAGAATTGACT+GGG + chr1.2:75030591-75030610 None:intergenic 35.0%
TGGTTTGAAAAACAAAGAGG+AGG - chr1.2:75031147-75031166 MS.gene34135:intron 35.0%
TGTTTATGCTCCTCAATTTC+AGG - chr1.2:75029546-75029565 MS.gene34135:intron 35.0%
TTAACAGATTGGGGTGAAAT+GGG - chr1.2:75030387-75030406 MS.gene34135:intron 35.0%
TTCTGTTCAACAGAATTGAC+TGG + chr1.2:75030592-75030611 None:intergenic 35.0%
TTGTTGAATGCTAAGATGAG+AGG + chr1.2:75029805-75029824 None:intergenic 35.0%
TTTAACAGATTGGGGTGAAA+TGG - chr1.2:75030386-75030405 MS.gene34135:intron 35.0%
! AACCATTTTCCTGTAGTTTC+AGG - chr1.2:75030475-75030494 MS.gene34135:intron 35.0%
! ACCATTTTCCTGTAGTTTCA+GGG - chr1.2:75030476-75030495 MS.gene34135:intron 35.0%
! GTGTTTGATCTTTTAGTGTC+AGG + chr1.2:75030139-75030158 None:intergenic 35.0%
! GTTAGGTTGTATGTCTTTGA+TGG - chr1.2:75030965-75030984 MS.gene34135:CDS 35.0%
! TTAAAAGGTCTTATGCTTCG+TGG - chr1.2:75030298-75030317 MS.gene34135:CDS 35.0%
!! CACTGGTTTGAAAAACAAAG+AGG - chr1.2:75031144-75031163 MS.gene34135:intron 35.0%
!! CTAGTGGATGAAAGATTTTG+AGG - chr1.2:75030686-75030705 MS.gene34135:intron 35.0%
!! GAACTTGATAGCTGTTTTCA+AGG + chr1.2:75030161-75030180 None:intergenic 35.0%
!! GTCTTTTAACTCACTACAAC+AGG - chr1.2:75029965-75029984 MS.gene34135:intron 35.0%
AACGAAATCCCAATGCTACT+GGG + chr1.2:75028807-75028826 None:intergenic 40.0%
AACTACATAGGCAACTACAG+TGG + chr1.2:75029701-75029720 None:intergenic 40.0%
ACCCTGAAACTACAGGAAAA+TGG + chr1.2:75030480-75030499 None:intergenic 40.0%
ACGAGGTGTATCAACTACAT+AGG + chr1.2:75029713-75029732 None:intergenic 40.0%
ACTACATAGGCAACTACAGT+GGG + chr1.2:75029700-75029719 None:intergenic 40.0%
AGATGAGGATGCTATAGATG+AGG - chr1.2:75031234-75031253 MS.gene34135:CDS 40.0%
ATATTCACATGGTCTGCTTC+TGG - chr1.2:75029640-75029659 MS.gene34135:intron 40.0%
CAACAGAATTGACTGGGTAT+TGG + chr1.2:75030585-75030604 None:intergenic 40.0%
CCTGTAGTTTCAGGGTTTAT+GGG - chr1.2:75030484-75030503 MS.gene34135:intron 40.0%
GAGGTAACCTTGTCTTATAG+AGG + chr1.2:75029786-75029805 None:intergenic 40.0%
GCATGTACCAAATGAAGTCA+AGG + chr1.2:75031345-75031364 None:intergenic 40.0%
GGTGAAATGGGAAGCAATAT+TGG - chr1.2:75030399-75030418 MS.gene34135:intron 40.0%
GTCATGAAGCAATTCAACCT+TGG - chr1.2:75029076-75029095 MS.gene34135:CDS 40.0%
GTGAATATCTCAATCTGACC+AGG + chr1.2:75029628-75029647 None:intergenic 40.0%
TATGTTCTTGTGGATACTCC+TGG - chr1.2:75029607-75029626 MS.gene34135:intron 40.0%
TCCTGTAGTTTCAGGGTTTA+TGG - chr1.2:75030483-75030502 MS.gene34135:intron 40.0%
TTATGTGATGAATCTCGACC+CGG - chr1.2:75028991-75029010 MS.gene34135:intron 40.0%
TTTAACTCAGAGCCTTTCTC+TGG - chr1.2:75030752-75030771 MS.gene34135:intron 40.0%
! ATCATTGTTGTTGGAATGGC+AGG - chr1.2:75028715-75028734 MS.gene34135:intron 40.0%
!! AGAAAGTACTTGAGAGTCAC+CGG + chr1.2:75028386-75028405 None:intergenic 40.0%
AAATCGCAGGAAGGAGAACA+TGG - chr1.2:75031069-75031088 MS.gene34135:intron 45.0%
AACTACAGTGGGAAATGTGG+AGG + chr1.2:75029689-75029708 None:intergenic 45.0%
AAGTGAATAAGCGGCGAGTT+TGG + chr1.2:75028409-75028428 None:intergenic 45.0%
ACAACATGAGTTTGCCTTGG+AGG - chr1.2:75029837-75029856 MS.gene34135:intron 45.0%
ACGTGAAGAGAAACAGCTGT+TGG - chr1.2:75031042-75031061 MS.gene34135:intron 45.0%
ACTCATCTGTGTTTAGGGAG+TGG - chr1.2:75028911-75028930 MS.gene34135:CDS 45.0%
AGATGAGGACGAAGATGAAG+AGG - chr1.2:75031249-75031268 MS.gene34135:CDS 45.0%
AGCACAACATGAGTTTGCCT+TGG - chr1.2:75029834-75029853 MS.gene34135:intron 45.0%
AGCTGTTGGAAGAAAATCGC+AGG - chr1.2:75031056-75031075 MS.gene34135:intron 45.0%
ATCGGAACTAGCGCTTGTAT+AGG - chr1.2:75031318-75031337 MS.gene34135:CDS 45.0%
ATTCATCCAGTGCCAGAGAA+AGG + chr1.2:75030767-75030786 None:intergenic 45.0%
CAAACTCAAGGAAAGCTACC+CGG + chr1.2:75029331-75029350 None:intergenic 45.0%
CAACGAAATCCCAATGCTAC+TGG + chr1.2:75028808-75028827 None:intergenic 45.0%
CCCATAAACCCTGAAACTAC+AGG + chr1.2:75030487-75030506 None:intergenic 45.0%
CTCATCTGTGTTTAGGGAGT+GGG - chr1.2:75028912-75028931 MS.gene34135:CDS 45.0%
GAAAAACAAAGAGGAGGACG+AGG - chr1.2:75031153-75031172 MS.gene34135:intron 45.0%
GAAGTTAGGTGAACCAGAAG+AGG + chr1.2:75028677-75028696 None:intergenic 45.0%
GACACAATCCCAGTAGCATT+GGG - chr1.2:75028796-75028815 MS.gene34135:intron 45.0%
GACATTGAAGGTTCCTCTTC+TGG - chr1.2:75028661-75028680 MS.gene34135:intron 45.0%
GACTACACTCATGCATAGGT+TGG - chr1.2:75028937-75028956 MS.gene34135:intron 45.0%
GAGTAACACTGTGTCTATCG+TGG + chr1.2:75028496-75028515 None:intergenic 45.0%
GGAAGACTACACTCATGCAT+AGG - chr1.2:75028933-75028952 MS.gene34135:intron 45.0%
GGATAAACTTAGGAGGGAGA+TGG - chr1.2:75031090-75031109 MS.gene34135:intron 45.0%
GGATGAAGAGGATGAGGAAA+TGG - chr1.2:75031174-75031193 MS.gene34135:intron 45.0%
GTTGGAAGAAAATCGCAGGA+AGG - chr1.2:75031060-75031079 MS.gene34135:intron 45.0%
GTTTGCAACCAAGTTCGATG+AGG - chr1.2:75029132-75029151 MS.gene34135:CDS 45.0%
TGACACAATCCCAGTAGCAT+TGG - chr1.2:75028795-75028814 MS.gene34135:intron 45.0%
TTCCATGTATTCCTCTGCAC+TGG + chr1.2:75030868-75030887 None:intergenic 45.0%
! CATTGTCAGTGGCTCGTTTT+AGG - chr1.2:75029426-75029445 MS.gene34135:intron 45.0%
! GAAACTGTAGTGTTGAGCAC+TGG - chr1.2:75031127-75031146 MS.gene34135:intron 45.0%
!! TATGGAAGTGTCATGACAGC+CGG + chr1.2:75029013-75029032 None:intergenic 45.0%
AAATTCCACCATTGGGGCCA+AGG + chr1.2:75029096-75029115 None:intergenic 50.0%
AATGGCAGGTCAGTCACGAA+AGG - chr1.2:75028729-75028748 MS.gene34135:intron 50.0%
AGCCAGTGCAGAGGAATACA+TGG - chr1.2:75030863-75030882 MS.gene34135:CDS 50.0%
AGGAGGGAGATGGAGAAATC+TGG - chr1.2:75031100-75031119 MS.gene34135:intron 50.0%
AGGGAGATGGAGAAATCTGG+AGG - chr1.2:75031103-75031122 MS.gene34135:intron 50.0%
CAATTCAACCTTGGCCCCAA+TGG - chr1.2:75029085-75029104 MS.gene34135:CDS 50.0%
CGGGACATACACTGAAGATG+AGG - chr1.2:75031219-75031238 MS.gene34135:intron 50.0%
GATCAGAACTTGCAGCTGCT+TGG + chr1.2:75030716-75030735 None:intergenic 50.0%
GCTGGAGTTTCTGCAGTTAC+CGG - chr1.2:75030807-75030826 MS.gene34135:intron 50.0%
GGCAACTACAGTGGGAAATG+TGG + chr1.2:75029692-75029711 None:intergenic 50.0%
GGCAGTCATTCGTACTACGA+AGG + chr1.2:75031374-75031393 None:intergenic 50.0%
! GTGGTAGGATTTTCTGCACG+AGG + chr1.2:75029730-75029749 None:intergenic 50.0%
!! ATGGAAGTGTCATGACAGCC+GGG + chr1.2:75029012-75029031 None:intergenic 50.0%
!! ATTTCCATGGGGGTGTCTTC+GGG + chr1.2:75028358-75028377 None:intergenic 50.0%
!! CATTTCCATGGGGGTGTCTT+CGG + chr1.2:75028359-75028378 None:intergenic 50.0%
!! TCACCGGCTTCCATTTCCAT+GGG + chr1.2:75028370-75028389 None:intergenic 50.0%
ACCCCCATGGAAATGGAAGC+CGG - chr1.2:75028364-75028383 MS.gene34135:CDS 55.0%
AGAGGAGGACGAGGATGAAG+AGG - chr1.2:75031162-75031181 MS.gene34135:intron 55.0%
AGCTGTTGAAGCCAGTGCAG+AGG - chr1.2:75030854-75030873 MS.gene34135:CDS 55.0%
CGAAGACACCCCCATGGAAA+TGG - chr1.2:75028357-75028376 MS.gene34135:CDS 55.0%
GAAGCCTTCGATTCCAGCAC+CGG + chr1.2:75030829-75030848 None:intergenic 55.0%
GCTGTGTCTGTGACGAGGTT+TGG - chr1.2:75030113-75030132 MS.gene34135:CDS 55.0%
GGACGAGGATGAAGAGGATG+AGG - chr1.2:75031168-75031187 MS.gene34135:intron 55.0%
GTTTCTGCAGTTACCGGTGC+TGG - chr1.2:75030813-75030832 MS.gene34135:CDS 55.0%
TCAGAGCCTTTCTCTGGCAC+TGG - chr1.2:75030758-75030777 MS.gene34135:intron 55.0%
TTCAACCTTGGCCCCAATGG+TGG - chr1.2:75029088-75029107 MS.gene34135:CDS 55.0%
TTTGGGCTGTGTCTGTGACG+AGG - chr1.2:75030108-75030127 MS.gene34135:CDS 55.0%
! ACCGGCTTCCATTTCCATGG+GGG + chr1.2:75028368-75028387 None:intergenic 55.0%
! GTTACCGGTGCTGGAATCGA+AGG - chr1.2:75030822-75030841 MS.gene34135:CDS 55.0%
!! CACCGGCTTCCATTTCCATG+GGG + chr1.2:75028369-75028388 None:intergenic 55.0%
!! GTCACCGGCTTCCATTTCCA+TGG + chr1.2:75028371-75028390 None:intergenic 55.0%
!! TTTCCATGGGGGTGTCTTCG+GGG + chr1.2:75028357-75028376 None:intergenic 55.0%
TAACCCCGAAGACACCCCCA+TGG - chr1.2:75028351-75028370 MS.gene34135:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 75028322 75031640 75028322 ID=MS.gene34135
chr1.2 mRNA 75028322 75031640 75028322 ID=MS.gene34135.t1;Parent=MS.gene34135
chr1.2 exon 75031566 75031640 75031566 ID=MS.gene34135.t1.exon1;Parent=MS.gene34135.t1
chr1.2 CDS 75031566 75031640 75031566 ID=cds.MS.gene34135.t1;Parent=MS.gene34135.t1
chr1.2 exon 75031226 75031349 75031226 ID=MS.gene34135.t1.exon2;Parent=MS.gene34135.t1
chr1.2 CDS 75031226 75031349 75031226 ID=cds.MS.gene34135.t1;Parent=MS.gene34135.t1
chr1.2 exon 75030809 75031035 75030809 ID=MS.gene34135.t1.exon3;Parent=MS.gene34135.t1
chr1.2 CDS 75030809 75031035 75030809 ID=cds.MS.gene34135.t1;Parent=MS.gene34135.t1
chr1.2 exon 75030104 75030394 75030104 ID=MS.gene34135.t1.exon4;Parent=MS.gene34135.t1
chr1.2 CDS 75030104 75030394 75030104 ID=cds.MS.gene34135.t1;Parent=MS.gene34135.t1
chr1.2 exon 75029067 75029272 75029067 ID=MS.gene34135.t1.exon5;Parent=MS.gene34135.t1
chr1.2 CDS 75029067 75029272 75029067 ID=cds.MS.gene34135.t1;Parent=MS.gene34135.t1
chr1.2 exon 75028814 75028938 75028814 ID=MS.gene34135.t1.exon6;Parent=MS.gene34135.t1
chr1.2 CDS 75028814 75028938 75028814 ID=cds.MS.gene34135.t1;Parent=MS.gene34135.t1
chr1.2 exon 75028473 75028486 75028473 ID=MS.gene34135.t1.exon7;Parent=MS.gene34135.t1
chr1.2 CDS 75028473 75028486 75028473 ID=cds.MS.gene34135.t1;Parent=MS.gene34135.t1
chr1.2 exon 75028322 75028384 75028322 ID=MS.gene34135.t1.exon8;Parent=MS.gene34135.t1
chr1.2 CDS 75028322 75028384 75028322 ID=cds.MS.gene34135.t1;Parent=MS.gene34135.t1
Gene Sequence

>MS.gene34135

ATGGACGCGGATAACGCTTCAAAGAACGCTAACCCCGAAGACACCCCCATGGAAATGGAAGCCGGTGACTCTCAAGTACTTTCTCCAAACTCGCCGCTTATTCACTTTCACTTTACTAATTCAAACAAAATTTAATTAGGTCAAACTTATATTCTGTTGTTGACTCACAAACCACGATAGACACAGTGTTACTCTATAAATGATAAACCCTAAAAACGTTTTTATTGTATTGTTGTAATTTTGCGAGTTTAATTTGAAATTGTGAATCATGGAATGGTTTTTGTTTTCAAGGAAAAAAACAAACAAAAAGAGGACCTGAGTGAGTCTATGAAGAAATTGGACATTGAAGGTTCCTCTTCTGGTTCACCTAACTTCAAAAGAAAACCTGTTATCATCATTGTTGTTGGAATGGCAGGTCAGTCACGAAAGGATTGCATTTACTTCATATTGCATGTTTGGTTTCGCAACGAGTTTGACACAATCCCAGTAGCATTGGGATTTCGTTGCGATACCAAACAAGCAAAGTATATTTTATTATTTCCCGTGTTAGTATTGTATACTATATGTAGTTTTCCTGACTTGGAATATAACTCATCTGTGTTTAGGGAGTGGGAAGACTACACTCATGCATAGGTTGGTTACACATACTCATCTGTCAAACATTAGGGGTTATGTGATGAATCTCGACCCGGCTGTCATGACACTTCCATATGCTTCTAACATTGATATAAGAGACACTGTTAAGTATAAAGAAGTCATGAAGCAATTCAACCTTGGCCCCAATGGTGGAATTTTGACTTCTCTTAACTTGTTTGCAACCAAGTTCGATGAGGTTAAGTTTTCTTATCAATACCTAAACTCATGTACAGTATAATATATATATTGCTGGTTATGGACTGATTGCATTTTGAATTCAGTTTTACTTATATATGCATTCGGTGGAAATTCATACCTCTTGATATCATTGATACTAGTAAACATGATCCCTGCTTAATTTTTTTTTATGCCGGGTAGCTTTCCTTGAGTTTGTTTGATCTTATGTTTTTGTTTTGTCCTCTAATTAAGCTATTGGTATAATGCTTGAGATAATAATGTTTACATTGTCATTGTCAGTGGCTCGTTTTAGGTTCTCATTTGTTGTTCTAGTCCAACAATTTATTTTTTTTCATGCCGATGTGTTTTACTTTTGTTCCCATCTACAGAACTTATATGCAATTTTACTAATGTTTATGCTCCTCAATTTCAGGTAATTTCTGTTATTGAGAAACGAGCAGATCAACTTGACTATGTTCTTGTGGATACTCCTGGTCAGATTGAGATATTCACATGGTCTGCTTCTGGTGCTATCATCACAGAAGCTTTTGCCTCCACATTTCCCACTGTAGTTGCCTATGTAGTTGATACACCTCGTGCAGAAAATCCTACCACTTTTATGAGCAACATGCTTTATGCTTGTAGTATCCTCTATAAGACAAGGTTACCTCTCATCTTAGCATTCAACAAGGTCGATGTAGCACAACATGAGTTTGCCTTGGAGGTATTGTTGATTTTGATGAATTTTCTTTAGCTTCAATTGTATGTAATGTATTGTTGAGATTAGTTCCTTGAAAGCTATAAAAATGATAGAATATGTTTCAGTGTTTGTCTTTTAACTCACTACAACAGGCGAGTTTAACCTGTGAGATCTAATTGATAGTTTTATCTCGGAAGTTGTTTAAACTACAAATTATTGTTGCCTTTTAAGTACTTAATTTGAACTATTAAGAGTGATAAATAAATCATGACATTTGGGCTGTGTCTGTGACGAGGTTTGGCCTGACACTAAAAGATCAAACACCTTGAAAACAGCTATCAAGTTCATGGCATTCAATTGTCCTATGAATTAAGAGTTAGACTTAATTGTTCTATTTTGTATATTTTATTTACCACTTAAGCATATTTTCTTGCCTCTCTCTAATTGCACATATAAATACTTATTAAAAGGTCTTATGCTTCGTGGATTTTAACATTTCATTCAATTGGTGAAAAATTGAGTTAAAACTAAAAAGTAATTCAACTTTTAAGTTTAACAGATTGGGGTGAAATGGGAAGCAATATTGGAGTTAGTTATTAGTTTTCCCTTTCATGTTTAAGTTATTATTTTATAGGCATAAAACCATTTTCCTGTAGTTTCAGGGTTTATGGGCCTCTTCCTTTGTATATTTGAGCTGAATCTATTTGTTTCTGCTCTGATAAATAAAACAGTTACACTATTTGCACTCCAATACCCAGTCAATTCTGTTGAACAGAACTTGTGTTTTGTCTTGTTTTTACATCTGCAACTGATTTTTTTTTCCTTCCTTTTGTCGAACTTGTTTTGTTTATCTAGTGGATGAAAGATTTTGAGGTATTCCAAGCAGCTGCAAGTTCTGATCAGTCATACACGTCAAATTTAACTCAGAGCCTTTCTCTGGCACTGGATGAATTTTACAGTAATTTAAGATCAGCTGGAGTTTCTGCAGTTACCGGTGCTGGAATCGAAGGCTTCTTCAAAGCTGTTGAAGCCAGTGCAGAGGAATACATGGAAACCTACAAGTATTAACTGCTCTTGACATATTTTCATTTGTTAGCTTCACCATTATTTCACTTTTGATTTTGCATTTAGTTAGGTTGTATGTCTTTGATGGCTAAGCAATTAACTTTTTCTATTATGCGATGTTTAGGGCTGATCTTGATAAAAGACGTGAAGAGAAACAGCTGTTGGAAGAAAATCGCAGGAAGGAGAACATGGATAAACTTAGGAGGGAGATGGAGAAATCTGGAGGAGAAACTGTAGTGTTGAGCACTGGTTTGAAAAACAAAGAGGAGGACGAGGATGAAGAGGATGAGGAAATGGATGATGATGATGATGTTGATTTCGGGACATACACTGAAGATGAGGATGCTATAGATGAGGACGAAGATGAAGAGGTTGATAAGTTTTCCTTCTGATGTGATTTTATGAACGTTTGGATTGCATCGGAACTAGCGCTTGTATAGGACCTTGACTTCATTTGGTACATGCATACTACCTTCGTAGTACGAATGACTGCCGATTTATTGCCCTGTTATTTCATACATTCTATCAGCATTATTCTCTCTAGGTTTTTTTCCTAGATGTTTTTTATACTAGTAGGAGTAATTGCAAAGGTAACCATGCTTGATTTTTGTGCAAATTGAGGACTAATGATTATCACGATGACTTATGACCCATATAACTAGTATCTGTTTTGTTTAGGGACAACAATGTGTAGAGTCTGAATGTGATACTATAGTGCTCATCTCCACATCCGCAATTTGA
Protein sequence

>MS.gene34135.t1

MDADNASKNANPEDTPMEMEAGDSQEKNKQKEDLSESMKKLDIEGSSSGSPNFKRKPVIIIVVGMAGSGKTTLMHRLVTHTHLSNIRGYVMNLDPAVMTLPYASNIDIRDTVKYKEVMKQFNLGPNGGILTSLNLFATKFDEVISVIEKRADQLDYVLVDTPGQIEIFTWSASGAIITEAFASTFPTVVAYVVDTPRAENPTTFMSNMLYACSILYKTRLPLILAFNKVDVAQHEFALEWMKDFEVFQAAASSDQSYTSNLTQSLSLALDEFYSNLRSAGVSAVTGAGIEGFFKAVEASAEEYMETYKADLDKRREEKQLLEENRRKENMDKLRREMEKSGGETVVLSTGVIAKGQQCVESECDTIVLISTSAI