AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34854


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34854.t1 MTR_8g062700 95.122 123 6 0 1 123 558 680 2.40e-76 249
MS.gene34854.t1 MTR_8g062700 95.122 123 6 0 1 123 559 681 2.41e-76 249
MS.gene34854.t1 MTR_5g036670 74.797 123 31 0 1 123 555 677 1.71e-60 204
MS.gene34854.t1 MTR_6g008970 64.228 123 44 0 1 123 541 663 3.38e-52 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34854.t1 AT1G24190 74.797 123 31 0 1 123 497 619 3.29e-61 206
MS.gene34854.t1 AT1G24190 74.797 123 31 0 1 123 493 615 3.50e-61 206
MS.gene34854.t1 AT1G24190 74.797 123 31 0 1 123 493 615 3.77e-61 206
MS.gene34854.t1 AT1G70060 73.171 123 33 0 1 123 511 633 3.52e-59 200
MS.gene34854.t1 AT1G70060 73.171 123 33 0 1 123 511 633 3.52e-59 200
MS.gene34854.t1 AT1G59890 73.984 123 32 0 1 123 386 508 2.19e-58 197
MS.gene34854.t1 AT1G59890 73.984 123 32 0 1 123 382 504 2.34e-58 197
MS.gene34854.t1 AT1G59890 73.984 123 32 0 1 123 391 513 2.47e-58 197
MS.gene34854.t1 AT1G59890 73.984 123 32 0 1 123 386 508 2.54e-58 197
MS.gene34854.t1 AT1G10450 70.732 123 36 0 1 123 369 491 2.32e-54 186
MS.gene34854.t1 AT1G10450 70.732 123 36 0 1 123 369 491 2.32e-54 186
MS.gene34854.t1 AT1G10450 70.732 123 36 0 1 123 420 542 2.69e-54 186
MS.gene34854.t1 AT5G15020 65.041 123 43 0 1 123 533 655 4.25e-52 179
MS.gene34854.t1 AT5G15020 65.041 123 43 0 1 123 533 655 4.50e-52 179
MS.gene34854.t1 AT3G01320 61.789 123 47 0 1 123 538 660 1.31e-48 169
MS.gene34854.t1 AT3G01320 61.789 123 47 0 1 123 526 648 1.39e-48 169

Find 49 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTATTGAGGAGCATTTATC+AGG 0.277753 8.2:+38688848 MS.gene34854:CDS
GTGCAGAGTTTAATATAGTT+TGG 0.279806 8.2:+38689129 MS.gene34854:CDS
GAATGCATCTCTAGCCTTGC+CGG 0.346779 8.2:+38689058 MS.gene34854:CDS
TGCAGAGTTTAATATAGTTT+GGG 0.348281 8.2:+38689130 MS.gene34854:CDS
ACTCCTCCTGTTTCTGCTTC+AGG 0.353246 8.2:-38689094 None:intergenic
GTTAATGATGATGTAATTCC+AGG 0.355314 8.2:+38688812 MS.gene34854:CDS
GGATGGTGCTGTTACTGTTC+AGG 0.386199 8.2:+38689787 MS.gene34854:CDS
AAATGCTCCTCAATACGAAT+TGG 0.386991 8.2:-38688842 None:intergenic
GAGCAGGCTGTCGACGGGAC+TGG 0.402053 8.2:+38689825 MS.gene34854:CDS
AACCCGCCTGAAGCAGAAAC+AGG 0.442327 8.2:+38689088 MS.gene34854:CDS
AGGTGCATTGAACGACTATA+CGG 0.453204 8.2:+38688999 MS.gene34854:CDS
ATACGAATTGGAATATCTCC+TGG 0.466439 8.2:-38688830 None:intergenic
AGATATTCCAATTCGTATTG+AGG 0.495294 8.2:+38688835 MS.gene34854:CDS
GAGAAAAGACATTATTTCAA+AGG 0.496041 8.2:+38689766 MS.gene34854:CDS
AAAGACATTATTTCAAAGGA+TGG 0.504914 8.2:+38689770 MS.gene34854:CDS
CGGGACTGGAGCGGAGATTG+TGG 0.507792 8.2:+38689839 MS.gene34854:CDS
CTCCTGTTTCTGCTTCAGGC+GGG 0.509322 8.2:-38689090 None:intergenic
AGTTACACCGGCGGGATCAC+TGG 0.518021 8.2:-38689729 None:intergenic
CAAATACTTGGCTGAAGTGA+AGG 0.518215 8.2:+38689679 MS.gene34854:CDS
TTCAGGCGGGTTAATATCAC+CGG 0.529652 8.2:-38689077 None:intergenic
CCTCCTGTTTCTGCTTCAGG+CGG 0.534175 8.2:-38689091 None:intergenic
GAAGGTGAGGAAGGCAGCCG+AGG 0.543484 8.2:+38689697 MS.gene34854:CDS
TTCTGAGAGAAACAAATACT+TGG 0.543933 8.2:+38689667 MS.gene34854:CDS
TGACCATGGGCTTGATGTGA+TGG 0.544947 8.2:+38689022 MS.gene34854:CDS
AGCTTCGAGCAGGCTGTCGA+CGG 0.546334 8.2:+38689819 MS.gene34854:CDS
GCTTCGAGCAGGCTGTCGAC+GGG 0.549946 8.2:+38689820 MS.gene34854:CDS
GTTGATACGGAGCTTCGAGC+AGG 0.550606 8.2:+38689809 MS.gene34854:CDS
GAACGACTATACGGTGACCA+TGG 0.550770 8.2:+38689008 MS.gene34854:CDS
TGTTACTGTTCAGGTTGATA+CGG 0.551073 8.2:+38689796 MS.gene34854:CDS
GGCTGAAGTGAAGGTGAGGA+AGG 0.554813 8.2:+38689688 MS.gene34854:CDS
GAAGTCGTCATGGGCAACAT+TGG 0.556203 8.2:-38689647 None:intergenic
AACGACTATACGGTGACCAT+GGG 0.556588 8.2:+38689009 MS.gene34854:CDS
TTTCTCTCAGAAGTCGTCAT+GGG 0.563812 8.2:-38689656 None:intergenic
GAAGCAGAAACAGGAGGAGT+GGG 0.580158 8.2:+38689097 MS.gene34854:CDS
GTTTCTCTCAGAAGTCGTCA+TGG 0.580421 8.2:-38689657 None:intergenic
AAACTATATTAAACTCTGCA+CGG 0.581815 8.2:-38689127 None:intergenic
TGAAGCAGAAACAGGAGGAG+TGG 0.583162 8.2:+38689096 MS.gene34854:CDS
GGCTGTCGACGGGACTGGAG+CGG 0.601171 8.2:+38689830 MS.gene34854:CDS
AAGGTGAGGAAGGCAGCCGA+GGG 0.608404 8.2:+38689698 MS.gene34854:CDS
TGCAACAAGTAAGAAAGTTG+AGG 0.611189 8.2:+38688775 MS.gene34854:CDS
TTACGATGAGTTACACCGGC+GGG 0.613809 8.2:-38689737 None:intergenic
ATCACTGGTAGCATATCCCT+CGG 0.627935 8.2:-38689714 None:intergenic
TGCTTTACGATGAGTTACAC+CGG 0.645166 8.2:-38689741 None:intergenic
CTTCCATCACATCAAGCCCA+TGG 0.664006 8.2:-38689025 None:intergenic
ACTTGGCTGAAGTGAAGGTG+AGG 0.666702 8.2:+38689684 MS.gene34854:CDS
GCGGGTTAATATCACCGGCA+AGG 0.668769 8.2:-38689072 None:intergenic
TATGCTACCAGTGATCCCGC+CGG 0.678025 8.2:+38689722 MS.gene34854:CDS
CCGCCTGAAGCAGAAACAGG+AGG 0.700575 8.2:+38689091 MS.gene34854:CDS
TTTACGATGAGTTACACCGG+CGG 0.801865 8.2:-38689738 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTCTTAACTATATTAAA+AGG - chr8.2:38689369-38689388 None:intergenic 10.0%
!! TTAAATAACTTACTATTTAA+AGG - chr8.2:38689316-38689335 None:intergenic 10.0%
!!! GAAATATTTAAAATTAGTTT+AGG + chr8.2:38689383-38689402 MS.gene34854:intron 10.0%
!! AATTTATCATAGCATATAAA+TGG + chr8.2:38688898-38688917 MS.gene34854:intron 15.0%
!!! AATCAAATATTTTCTTTCAA+TGG - chr8.2:38689342-38689361 None:intergenic 15.0%
!! TATTTAAAGGTTGTTAAAGA+GGG - chr8.2:38689303-38689322 None:intergenic 20.0%
!!! CAATTCTTGATTCTTTTTAA+CGG + chr8.2:38689272-38689291 MS.gene34854:intron 20.0%
! AAACTATATTAAACTCTGCA+CGG - chr8.2:38689130-38689149 None:intergenic 25.0%
! AAAGACATTATTTCAAAGGA+TGG + chr8.2:38689770-38689789 MS.gene34854:CDS 25.0%
! AGAATCAAGAATTGTGTTAT+TGG - chr8.2:38689266-38689285 None:intergenic 25.0%
! ATAAACTCTTGAATAAGGAA+GGG + chr8.2:38689515-38689534 MS.gene34854:intron 25.0%
! ATTCAAGAGTTTATGGATAT+CGG - chr8.2:38689509-38689528 None:intergenic 25.0%
! CTATTTAAAGGTTGTTAAAG+AGG - chr8.2:38689304-38689323 None:intergenic 25.0%
! GAGAAAAGACATTATTTCAA+AGG + chr8.2:38689766-38689785 MS.gene34854:CDS 25.0%
! TATCCATAAACTCTTGAATA+AGG + chr8.2:38689510-38689529 MS.gene34854:intron 25.0%
! TGCAGAGTTTAATATAGTTT+GGG + chr8.2:38689130-38689149 MS.gene34854:CDS 25.0%
! TGTCAACAAATAATGAAAAG+AGG - chr8.2:38689608-38689627 None:intergenic 25.0%
!!! TTGTTTTAGCTCTAAATCTT+AGG + chr8.2:38688979-38688998 MS.gene34854:intron 25.0%
AGAACAGAACACAAAATATC+AGG - chr8.2:38688939-38688958 None:intergenic 30.0%
AGATATTCCAATTCGTATTG+AGG + chr8.2:38688835-38688854 MS.gene34854:CDS 30.0%
CATAAACTCTTGAATAAGGA+AGG + chr8.2:38689514-38689533 MS.gene34854:intron 30.0%
CTTCCTTATTCAAGAGTTTA+TGG - chr8.2:38689516-38689535 None:intergenic 30.0%
GTGCAGAGTTTAATATAGTT+TGG + chr8.2:38689129-38689148 MS.gene34854:CDS 30.0%
TAAACTCTTGAATAAGGAAG+GGG + chr8.2:38689516-38689535 MS.gene34854:intron 30.0%
! CTTGATTCTTTTTAACGGTA+TGG + chr8.2:38689277-38689296 MS.gene34854:intron 30.0%
! GTTAATGATGATGTAATTCC+AGG + chr8.2:38688812-38688831 MS.gene34854:CDS 30.0%
! TTCTGAGAGAAACAAATACT+TGG + chr8.2:38689667-38689686 MS.gene34854:CDS 30.0%
AAATGCTCCTCAATACGAAT+TGG - chr8.2:38688845-38688864 None:intergenic 35.0%
ACAAAAAGCTTGAGCACTAA+AGG + chr8.2:38689221-38689240 MS.gene34854:CDS 35.0%
ATACGAATTGGAATATCTCC+TGG - chr8.2:38688833-38688852 None:intergenic 35.0%
CTGTTCTGATACTATCTTCT+TGG + chr8.2:38688952-38688971 MS.gene34854:intron 35.0%
TGCAACAAGTAAGAAAGTTG+AGG + chr8.2:38688775-38688794 MS.gene34854:CDS 35.0%
! AAGCTTTTACTTTAAGCAGC+AGG + chr8.2:38689196-38689215 MS.gene34854:CDS 35.0%
!! TGTTACTGTTCAGGTTGATA+CGG + chr8.2:38689796-38689815 MS.gene34854:CDS 35.0%
AGCCCAAACCAATATTAGCA+GGG + chr8.2:38689451-38689470 MS.gene34854:intron 40.0%
AGGTGCATTGAACGACTATA+CGG + chr8.2:38688999-38689018 MS.gene34854:CDS 40.0%
CAAATACTTGGCTGAAGTGA+AGG + chr8.2:38689679-38689698 MS.gene34854:CDS 40.0%
CTTGAATAAGGAAGGGGATA+CGG + chr8.2:38689522-38689541 MS.gene34854:intron 40.0%
TGCTTTACGATGAGTTACAC+CGG - chr8.2:38689744-38689763 None:intergenic 40.0%
TTTCTCTCAGAAGTCGTCAT+GGG - chr8.2:38689659-38689678 None:intergenic 40.0%
!! CGTATTGAGGAGCATTTATC+AGG + chr8.2:38688848-38688867 MS.gene34854:CDS 40.0%
AAACCAATATTAGCAGGGCG+TGG + chr8.2:38689456-38689475 MS.gene34854:intron 45.0%
AACGACTATACGGTGACCAT+GGG + chr8.2:38689009-38689028 MS.gene34854:CDS 45.0%
ATCACTGGTAGCATATCCCT+CGG - chr8.2:38689717-38689736 None:intergenic 45.0%
CAGCCCAAACCAATATTAGC+AGG + chr8.2:38689450-38689469 MS.gene34854:intron 45.0%
GTTTCTCTCAGAAGTCGTCA+TGG - chr8.2:38689660-38689679 None:intergenic 45.0%
TTTACGATGAGTTACACCGG+CGG - chr8.2:38689741-38689760 None:intergenic 45.0%
! ACGCCCTGCTAATATTGGTT+TGG - chr8.2:38689457-38689476 None:intergenic 45.0%
!! CGCCCTGCTAATATTGGTTT+GGG - chr8.2:38689456-38689475 None:intergenic 45.0%
!! TTCAGGCGGGTTAATATCAC+CGG - chr8.2:38689080-38689099 None:intergenic 45.0%
!!! AAATATTTAAAATTAGTTTA+GGG + chr8.2:38689384-38689403 MS.gene34854:intron 5.0%
ACTTGGCTGAAGTGAAGGTG+AGG + chr8.2:38689684-38689703 MS.gene34854:CDS 50.0%
CAATATTAGCAGGGCGTGGT+GGG + chr8.2:38689460-38689479 MS.gene34854:intron 50.0%
CTTCCATCACATCAAGCCCA+TGG - chr8.2:38689028-38689047 None:intergenic 50.0%
GAACGACTATACGGTGACCA+TGG + chr8.2:38689008-38689027 MS.gene34854:CDS 50.0%
GAAGCAGAAACAGGAGGAGT+GGG + chr8.2:38689097-38689116 MS.gene34854:CDS 50.0%
GAAGTCGTCATGGGCAACAT+TGG - chr8.2:38689650-38689669 None:intergenic 50.0%
GAATAAGGAAGGGGATACGG+AGG + chr8.2:38689525-38689544 MS.gene34854:intron 50.0%
TGAAGCAGAAACAGGAGGAG+TGG + chr8.2:38689096-38689115 MS.gene34854:CDS 50.0%
TGACCATGGGCTTGATGTGA+TGG + chr8.2:38689022-38689041 MS.gene34854:CDS 50.0%
TTACGATGAGTTACACCGGC+GGG - chr8.2:38689740-38689759 None:intergenic 50.0%
!! ACTCCTCCTGTTTCTGCTTC+AGG - chr8.2:38689097-38689116 None:intergenic 50.0%
!! GAATGCATCTCTAGCCTTGC+CGG + chr8.2:38689058-38689077 MS.gene34854:CDS 50.0%
!! GGATGGTGCTGTTACTGTTC+AGG + chr8.2:38689787-38689806 MS.gene34854:CDS 50.0%
AACCCGCCTGAAGCAGAAAC+AGG + chr8.2:38689088-38689107 MS.gene34854:CDS 55.0%
CCAATATTAGCAGGGCGTGG+TGG + chr8.2:38689459-38689478 MS.gene34854:intron 55.0%
CCACCACGCCCTGCTAATAT+TGG - chr8.2:38689462-38689481 None:intergenic 55.0%
GGCTGAAGTGAAGGTGAGGA+AGG + chr8.2:38689688-38689707 MS.gene34854:CDS 55.0%
GTTGATACGGAGCTTCGAGC+AGG + chr8.2:38689809-38689828 MS.gene34854:CDS 55.0%
TATGCTACCAGTGATCCCGC+CGG + chr8.2:38689722-38689741 MS.gene34854:CDS 55.0%
! GCGGGTTAATATCACCGGCA+AGG - chr8.2:38689075-38689094 None:intergenic 55.0%
!! CCTCCTGTTTCTGCTTCAGG+CGG - chr8.2:38689094-38689113 None:intergenic 55.0%
!! CTCCTGTTTCTGCTTCAGGC+GGG - chr8.2:38689093-38689112 None:intergenic 55.0%
AAGGTGAGGAAGGCAGCCGA+GGG + chr8.2:38689698-38689717 MS.gene34854:CDS 60.0%
AGCTTCGAGCAGGCTGTCGA+CGG + chr8.2:38689819-38689838 MS.gene34854:CDS 60.0%
AGTTACACCGGCGGGATCAC+TGG - chr8.2:38689732-38689751 None:intergenic 60.0%
CCGCCTGAAGCAGAAACAGG+AGG + chr8.2:38689091-38689110 MS.gene34854:CDS 60.0%
CGGGACTGGAGCGGAGATTG+TGG + chr8.2:38689839-38689858 MS.gene34854:CDS 65.0%
GAAGGTGAGGAAGGCAGCCG+AGG + chr8.2:38689697-38689716 MS.gene34854:CDS 65.0%
GCTTCGAGCAGGCTGTCGAC+GGG + chr8.2:38689820-38689839 MS.gene34854:CDS 65.0%
GAGCAGGCTGTCGACGGGAC+TGG + chr8.2:38689825-38689844 MS.gene34854:CDS 70.0%
GGCTGTCGACGGGACTGGAG+CGG + chr8.2:38689830-38689849 MS.gene34854:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 38688755 38689871 38688755 ID=MS.gene34854
chr8.2 mRNA 38688755 38689871 38688755 ID=MS.gene34854.t1;Parent=MS.gene34854
chr8.2 exon 38688755 38688869 38688755 ID=MS.gene34854.t1.exon1;Parent=MS.gene34854.t1
chr8.2 CDS 38688755 38688869 38688755 ID=cds.MS.gene34854.t1;Parent=MS.gene34854.t1
chr8.2 exon 38688988 38689242 38688988 ID=MS.gene34854.t1.exon2;Parent=MS.gene34854.t1
chr8.2 CDS 38688988 38689242 38688988 ID=cds.MS.gene34854.t1;Parent=MS.gene34854.t1
chr8.2 exon 38689645 38689871 38689645 ID=MS.gene34854.t1.exon3;Parent=MS.gene34854.t1
chr8.2 CDS 38689645 38689871 38689645 ID=cds.MS.gene34854.t1;Parent=MS.gene34854.t1
Gene Sequence

>MS.gene34854

ATGTTGTTAGAGTCTATAAATGCAACAAGTAAGAAAGTTGAGGAGATCATAGAAAAAGTTAATGATGATGTAATTCCAGGAGATATTCCAATTCGTATTGAGGAGCATTTATCAGGTTATATGTTTTTTCAATTGATCAATGAAATTTATCATAGCATATAAATGGAAAATGATGTAATTTCCTGATATTTTGTGTTCTGTTCTGATACTATCTTCTTGGTGTGTTGTTTTAGCTCTAAATCTTAGGTGCATTGAACGACTATACGGTGACCATGGGCTTGATGTGATGGAAGTGTTGAAAAAGAATGCATCTCTAGCCTTGCCGGTGATATTAACCCGCCTGAAGCAGAAACAGGAGGAGTGGGCAAGATGCCGTGCAGAGTTTAATATAGTTTGGGCTGATGTATATGCGAAGAATTATCATAAATCACTTGATCATCGAAGCTTTTACTTTAAGCAGCAGGATACAAAAAGCTTGAGCACTAAAGGTAAAATATTAATTTTCTGTCCAATAACACAATTCTTGATTCTTTTTAACGGTATGGCCCTCTTTAACAACCTTTAAATAGTAAGTTATTTAACATCCATTGAAAGAAAATATTTGATTTAATCCTTTTAATATAGTTAAGAAATATTTAAAATTAGTTTAGGGAATAAAGTATACTTAATTAATTTAATGTAGTTAGTTAAGAACACAGCCCAAACCAATATTAGCAGGGCGTGGTGGGCATTTTAGACAAGATGTTGCTCTCCGATATCCATAAACTCTTGAATAAGGAAGGGGATACGGAGGATCTAAATGAATATTTGCGTGAGTGTTTTTGTCTGAGAATTGCTTTCTTCATGCTTGCCTCTTTTCATTATTTGTTGACATTGTTTTTATTTTGTAGAACCAATGTTGCCCATGACGACTTCTGAGAGAAACAAATACTTGGCTGAAGTGAAGGTGAGGAAGGCAGCCGAGGGATATGCTACCAGTGATCCCGCCGGTGTAACTCATCGTAAAGCAAAGAGAAAAGACATTATTTCAAAGGATGGTGCTGTTACTGTTCAGGTTGATACGGAGCTTCGAGCAGGCTGTCGACGGGACTGGAGCGGAGATTGTGGATTCTCCTAA

Protein sequence

>MS.gene34854.t1

MLLESINATSKKVEEIIEKVNDDVIPGDIPIRIEEHLSALNLRCIERLYGDHGLDVMEVLKKNASLALPVILTRLKQKQEEWARCRAEFNIVWADVYAKNYHKSLDHRSFYFKQQDTKSLSTKEPMLPMTTSERNKYLAEVKVRKAAEGYATSDPAGVTHRKAKRKDIISKDGAVTVQVDTELRAGCRRDWSGDCGFS