Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35049.t1 | PNX82701.1 | 56 | 225 | 91 | 1 | 1 | 217 | 1 | 225 | 4.80E-60 | 241.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35049.t1 | Q9SJ52 | 38.6 | 220 | 125 | 3 | 2 | 219 | 3 | 214 | 6.1e-40 | 165.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35049.t1 | A0A2K3LVY2 | 56.0 | 225 | 91 | 1 | 1 | 217 | 1 | 225 | 3.4e-60 | 241.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene35049 | MS.gene007146 | PPI |
| MS.gene35049 | MS.gene70399 | PPI |
| MS.gene050363 | MS.gene35049 | PPI |
| MS.gene35049 | MS.gene036507 | PPI |
| MS.gene35049 | MS.gene041542 | PPI |
| MS.gene35049 | MS.gene58402 | PPI |
| MS.gene35049 | MS.gene028407 | PPI |
| MS.gene35049 | MS.gene034789 | PPI |
| MS.gene35049 | MS.gene41069 | PPI |
| MS.gene35049 | MS.gene025640 | PPI |
| MS.gene35049 | MS.gene055657 | PPI |
| MS.gene35049 | MS.gene055658 | PPI |
| MS.gene35049 | MS.gene036506 | PPI |
| MS.gene35049 | MS.gene051327 | PPI |
| MS.gene35049 | MS.gene035313 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35049.t1 | MTR_4g119428 | 65.198 | 227 | 69 | 4 | 1 | 219 | 2 | 226 | 6.11e-94 | 275 |
| MS.gene35049.t1 | MTR_1g057270 | 56.140 | 228 | 91 | 2 | 1 | 219 | 1 | 228 | 2.84e-72 | 220 |
| MS.gene35049.t1 | MTR_7g106000 | 39.091 | 220 | 117 | 4 | 1 | 213 | 1 | 210 | 1.66e-48 | 159 |
| MS.gene35049.t1 | MTR_7g106010 | 39.450 | 218 | 119 | 3 | 5 | 218 | 4 | 212 | 3.28e-48 | 158 |
| MS.gene35049.t1 | MTR_7g105870 | 46.411 | 209 | 105 | 2 | 4 | 211 | 5 | 207 | 2.91e-47 | 155 |
| MS.gene35049.t1 | MTR_7g106030 | 36.530 | 219 | 125 | 4 | 5 | 218 | 7 | 216 | 3.25e-41 | 140 |
| MS.gene35049.t1 | MTR_7g105970 | 36.036 | 222 | 125 | 4 | 1 | 215 | 1 | 212 | 1.44e-37 | 130 |
| MS.gene35049.t1 | MTR_1g057400 | 35.714 | 182 | 112 | 2 | 38 | 214 | 11 | 192 | 1.60e-34 | 122 |
| MS.gene35049.t1 | MTR_6g071410 | 36.207 | 174 | 101 | 6 | 45 | 211 | 9 | 179 | 2.08e-25 | 99.0 |
| MS.gene35049.t1 | MTR_6g070890 | 36.207 | 174 | 101 | 6 | 45 | 211 | 9 | 179 | 2.08e-25 | 99.0 |
| MS.gene35049.t1 | MTR_6g471090 | 35.979 | 189 | 103 | 8 | 30 | 212 | 2 | 178 | 7.09e-25 | 97.4 |
| MS.gene35049.t1 | MTR_6g070900 | 34.483 | 174 | 103 | 6 | 47 | 211 | 8 | 179 | 2.90e-22 | 90.9 |
| MS.gene35049.t1 | MTR_6g071400 | 34.483 | 174 | 103 | 6 | 47 | 211 | 8 | 179 | 2.90e-22 | 90.9 |
| MS.gene35049.t1 | MTR_4g094322 | 75.510 | 49 | 12 | 0 | 171 | 219 | 2 | 50 | 1.46e-20 | 82.0 |
| MS.gene35049.t1 | MTR_2g011350 | 29.252 | 147 | 103 | 1 | 59 | 205 | 35 | 180 | 4.59e-20 | 84.7 |
| MS.gene35049.t1 | MTR_1g057290 | 39.333 | 150 | 86 | 2 | 72 | 216 | 52 | 201 | 1.29e-19 | 84.0 |
| MS.gene35049.t1 | MTR_6g471080 | 37.647 | 170 | 98 | 6 | 48 | 211 | 7 | 174 | 1.01e-17 | 78.6 |
| MS.gene35049.t1 | MTR_3g078800 | 29.814 | 161 | 110 | 3 | 51 | 209 | 73 | 232 | 2.57e-17 | 78.6 |
| MS.gene35049.t1 | MTR_2g048270 | 38.686 | 137 | 76 | 5 | 87 | 218 | 3 | 136 | 4.87e-17 | 75.9 |
| MS.gene35049.t1 | MTR_5g087760 | 26.000 | 200 | 126 | 7 | 23 | 214 | 55 | 240 | 1.08e-16 | 76.6 |
| MS.gene35049.t1 | MTR_5g076170 | 32.051 | 156 | 102 | 4 | 63 | 216 | 96 | 249 | 1.22e-16 | 76.6 |
| MS.gene35049.t1 | MTR_3g063310 | 30.137 | 146 | 98 | 3 | 66 | 209 | 87 | 230 | 5.78e-16 | 74.7 |
| MS.gene35049.t1 | MTR_7g106100 | 32.203 | 118 | 73 | 3 | 42 | 153 | 64 | 180 | 1.91e-13 | 67.8 |
| MS.gene35049.t1 | MTR_2g100060 | 25.170 | 147 | 108 | 2 | 61 | 206 | 78 | 223 | 2.35e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35049.t1 | AT2G35460 | 38.182 | 220 | 128 | 3 | 4 | 215 | 3 | 222 | 4.85e-55 | 176 |
| MS.gene35049.t1 | AT2G35980 | 38.679 | 212 | 120 | 3 | 2 | 211 | 3 | 206 | 4.98e-53 | 170 |
| MS.gene35049.t1 | AT3G11650 | 37.443 | 219 | 129 | 3 | 6 | 217 | 8 | 225 | 1.35e-51 | 167 |
| MS.gene35049.t1 | AT5G06320 | 39.450 | 218 | 127 | 3 | 1 | 215 | 1 | 216 | 4.30e-48 | 158 |
| MS.gene35049.t1 | AT1G32270 | 33.775 | 151 | 98 | 1 | 69 | 217 | 1 | 151 | 6.86e-22 | 92.0 |
| MS.gene35049.t1 | AT1G32270 | 33.775 | 151 | 98 | 1 | 69 | 217 | 1 | 151 | 1.87e-21 | 91.7 |
| MS.gene35049.t1 | AT5G22870 | 25.767 | 163 | 117 | 3 | 46 | 205 | 24 | 185 | 3.34e-16 | 74.3 |
| MS.gene35049.t1 | AT4G01410 | 25.625 | 160 | 119 | 0 | 48 | 207 | 44 | 203 | 1.17e-15 | 73.6 |
| MS.gene35049.t1 | AT4G05220 | 22.051 | 195 | 149 | 3 | 16 | 209 | 14 | 206 | 1.64e-15 | 73.2 |
| MS.gene35049.t1 | AT3G52470 | 28.000 | 150 | 106 | 2 | 34 | 182 | 5 | 153 | 8.19e-15 | 70.9 |
| MS.gene35049.t1 | AT5G53730 | 28.402 | 169 | 108 | 4 | 61 | 217 | 40 | 207 | 8.19e-15 | 70.9 |
| MS.gene35049.t1 | AT2G27080 | 25.248 | 202 | 133 | 6 | 15 | 209 | 49 | 239 | 1.62e-14 | 70.9 |
| MS.gene35049.t1 | AT2G27080 | 25.248 | 202 | 133 | 6 | 15 | 209 | 49 | 239 | 1.62e-14 | 70.9 |
| MS.gene35049.t1 | AT5G21130 | 25.743 | 202 | 132 | 6 | 15 | 209 | 70 | 260 | 1.15e-12 | 65.9 |
| MS.gene35049.t1 | AT4G09590 | 25.000 | 160 | 118 | 2 | 48 | 206 | 22 | 180 | 1.31e-12 | 64.7 |
| MS.gene35049.t1 | AT1G65690 | 25.444 | 169 | 122 | 4 | 49 | 215 | 71 | 237 | 5.10e-12 | 63.9 |
| MS.gene35049.t1 | AT1G61760 | 23.353 | 167 | 125 | 3 | 44 | 209 | 40 | 204 | 2.28e-11 | 61.6 |
| MS.gene35049.t1 | AT5G36970 | 25.000 | 152 | 109 | 4 | 60 | 209 | 78 | 226 | 7.43e-11 | 60.5 |
Find 43 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATAATTGGTTTCCTCTTT+TGG | 0.148150 | 4.4:-20493684 | MS.gene35049:CDS |
| GCAAAACACTTGTATTCTTA+TGG | 0.189397 | 4.4:+20493440 | None:intergenic |
| GGCTTCTGCAGTTGCTTTAG+AGG | 0.296458 | 4.4:-20493769 | MS.gene35049:CDS |
| CAAAACACTTGTATTCTTAT+GGG | 0.307477 | 4.4:+20493441 | None:intergenic |
| TTCAAATCGCAATGAAGTTT+AGG | 0.324721 | 4.4:+20493262 | None:intergenic |
| CAAGGGATTCATTTGAGTTC+AGG | 0.328368 | 4.4:-20493397 | MS.gene35049:CDS |
| ATTGTCATAGTAGCAATAAT+TGG | 0.352334 | 4.4:-20493697 | MS.gene35049:CDS |
| TAAACTTCATTGCGATTTGA+AGG | 0.374826 | 4.4:-20493260 | MS.gene35049:CDS |
| CGCTGGGCTTGTTTAGGTCT+AGG | 0.393225 | 4.4:-20493294 | MS.gene35049:CDS |
| CTTCACTAACGCTGAATTTG+AGG | 0.395049 | 4.4:+20493641 | None:intergenic |
| TCTAAATTTGAGGTGTACGC+TGG | 0.411534 | 4.4:-20493311 | MS.gene35049:CDS |
| GGTGTACGCTGGGCTTGTTT+AGG | 0.415997 | 4.4:-20493300 | MS.gene35049:CDS |
| AAGTTTGTTTCTCAAACCTT+AGG | 0.419715 | 4.4:-20493478 | MS.gene35049:CDS |
| TGCAGTTGCTTTAGAGGATG+TGG | 0.422429 | 4.4:-20493763 | MS.gene35049:CDS |
| TCAAATGTCAGAGTTCAATA+AGG | 0.431524 | 4.4:-20493374 | MS.gene35049:CDS |
| GCAGAAGCCGCAACCATTGT+TGG | 0.436398 | 4.4:+20493783 | None:intergenic |
| CAAAACCCTAATCGAAAAGT+TGG | 0.457401 | 4.4:-20493547 | MS.gene35049:CDS |
| TTCGACGGCGAGGGGATGGA+AGG | 0.471157 | 4.4:+20493826 | None:intergenic |
| ACGCTGAATTTGAGGACGTA+GGG | 0.476124 | 4.4:+20493649 | None:intergenic |
| AACCATTGTTGGTGTGGCGT+CGG | 0.479384 | 4.4:+20493794 | None:intergenic |
| CGGTCAGATTTCGACGGCGA+GGG | 0.491291 | 4.4:+20493817 | None:intergenic |
| AGATTTCGACGGCGAGGGGA+TGG | 0.498045 | 4.4:+20493822 | None:intergenic |
| AACAATGGAACTTCTTCGTA+GGG | 0.531313 | 4.4:-20493223 | None:intergenic |
| CATTGTTGGTGTGGCGTCGG+CGG | 0.541260 | 4.4:+20493797 | None:intergenic |
| GTTCCATTGTTTGATGTCAA+CGG | 0.541893 | 4.4:+20493235 | None:intergenic |
| CTAAATTTGAGGTGTACGCT+GGG | 0.543006 | 4.4:-20493310 | MS.gene35049:CDS |
| CGCCACACCAACAATGGTTG+CGG | 0.544206 | 4.4:-20493790 | MS.gene35049:CDS |
| AAACAATGGAACTTCTTCGT+AGG | 0.544398 | 4.4:-20493224 | None:intergenic |
| CGCCGACGCCACACCAACAA+TGG | 0.545022 | 4.4:-20493796 | MS.gene35049:CDS |
| AAAACACTTGTATTCTTATG+GGG | 0.553234 | 4.4:+20493442 | None:intergenic |
| GGCGCACAATAAACCAAAAG+AGG | 0.561827 | 4.4:+20493671 | None:intergenic |
| AGCCGCAACCATTGTTGGTG+TGG | 0.573197 | 4.4:+20493788 | None:intergenic |
| TCGACGGCGAGGGGATGGAA+GGG | 0.573313 | 4.4:+20493827 | None:intergenic |
| CAACTCCACAATGCTTACTA+CGG | 0.575454 | 4.4:-20493850 | MS.gene35049:CDS |
| GGTCAGATTTCGACGGCGAG+GGG | 0.601595 | 4.4:+20493818 | None:intergenic |
| TGCTACTATGACAATGATTG+TGG | 0.622752 | 4.4:+20493705 | None:intergenic |
| GCGGTCAGATTTCGACGGCG+AGG | 0.623861 | 4.4:+20493816 | None:intergenic |
| AGTTGCTTTAGAGGATGTGG+TGG | 0.630447 | 4.4:-20493760 | MS.gene35049:CDS |
| CGTCGGCGGTCAGATTTCGA+CGG | 0.641839 | 4.4:+20493811 | None:intergenic |
| CGCTGAATTTGAGGACGTAG+GGG | 0.649005 | 4.4:+20493650 | None:intergenic |
| AAGGGCCGTAGTAAGCATTG+TGG | 0.684765 | 4.4:+20493845 | None:intergenic |
| GTTCCGTTGACATCAAACAA+TGG | 0.691708 | 4.4:-20493238 | MS.gene35049:CDS |
| AACGCTGAATTTGAGGACGT+AGG | 0.716355 | 4.4:+20493648 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAACACTTGTATTCTTATG+GGG | + | chr4.4:20493648-20493667 | None:intergenic | 25.0% |
| ! | ATTGTCATAGTAGCAATAAT+TGG | - | chr4.4:20493390-20493409 | MS.gene35049:CDS | 25.0% |
| ! | CAAAACACTTGTATTCTTAT+GGG | + | chr4.4:20493649-20493668 | None:intergenic | 25.0% |
| ! | TTCAATAAGGAGAAAAATGA+TGG | - | chr4.4:20493726-20493745 | MS.gene35049:CDS | 25.0% |
| !! | ATAAGAATACAAGTGTTTTG+CGG | - | chr4.4:20493649-20493668 | MS.gene35049:CDS | 25.0% |
| !! | TGAAGGTTTTTCTAAATTTG+AGG | - | chr4.4:20493766-20493785 | MS.gene35049:CDS | 25.0% |
| GCAAAACACTTGTATTCTTA+TGG | + | chr4.4:20493650-20493669 | None:intergenic | 30.0% | |
| TAAACTTCATTGCGATTTGA+AGG | - | chr4.4:20493827-20493846 | MS.gene35049:CDS | 30.0% | |
| TCAAATGTCAGAGTTCAATA+AGG | - | chr4.4:20493713-20493732 | MS.gene35049:CDS | 30.0% | |
| TTCAAATCGCAATGAAGTTT+AGG | + | chr4.4:20493828-20493847 | None:intergenic | 30.0% | |
| ! | AAGTTTGTTTCTCAAACCTT+AGG | - | chr4.4:20493609-20493628 | MS.gene35049:CDS | 30.0% |
| ! | TAAGAATACAAGTGTTTTGC+GGG | - | chr4.4:20493650-20493669 | MS.gene35049:CDS | 30.0% |
| !! | CAATAATTGGTTTCCTCTTT+TGG | - | chr4.4:20493403-20493422 | MS.gene35049:CDS | 30.0% |
| CAAAACCCTAATCGAAAAGT+TGG | - | chr4.4:20493540-20493559 | MS.gene35049:CDS | 35.0% | |
| GTTCCATTGTTTGATGTCAA+CGG | + | chr4.4:20493855-20493874 | None:intergenic | 35.0% | |
| TGCTACTATGACAATGATTG+TGG | + | chr4.4:20493385-20493404 | None:intergenic | 35.0% | |
| ! | CAACTTTTCGATTAGGGTTT+TGG | + | chr4.4:20493542-20493561 | None:intergenic | 35.0% |
| ! | CGATACCAACTTTTCGATTA+GGG | + | chr4.4:20493548-20493567 | None:intergenic | 35.0% |
| ! | GGGTTGAAAGAATTTTCCTA+AGG | + | chr4.4:20493628-20493647 | None:intergenic | 35.0% |
| ! | TCGATACCAACTTTTCGATT+AGG | + | chr4.4:20493549-20493568 | None:intergenic | 35.0% |
| !! | CTGCTTTTAAAGGTAAGCAA+GGG | - | chr4.4:20493673-20493692 | MS.gene35049:CDS | 35.0% |
| !! | GAGAAAAATGATGGCGTTTT+TGG | - | chr4.4:20493735-20493754 | MS.gene35049:CDS | 35.0% |
| !!! | CGTTTTTGGAATTGACTTGA+AGG | - | chr4.4:20493749-20493768 | MS.gene35049:CDS | 35.0% |
| CAACTCCACAATGCTTACTA+CGG | - | chr4.4:20493237-20493256 | MS.gene35049:CDS | 40.0% | |
| CTTCACTAACGCTGAATTTG+AGG | + | chr4.4:20493449-20493468 | None:intergenic | 40.0% | |
| GTTCCGTTGACATCAAACAA+TGG | - | chr4.4:20493849-20493868 | MS.gene35049:CDS | 40.0% | |
| ! | CAAGGGATTCATTTGAGTTC+AGG | - | chr4.4:20493690-20493709 | MS.gene35049:CDS | 40.0% |
| ! | CTAAATTTGAGGTGTACGCT+GGG | - | chr4.4:20493777-20493796 | MS.gene35049:CDS | 40.0% |
| ! | TCTAAATTTGAGGTGTACGC+TGG | - | chr4.4:20493776-20493795 | MS.gene35049:CDS | 40.0% |
| !!! | GCTGCTTTTAAAGGTAAGCA+AGG | - | chr4.4:20493672-20493691 | MS.gene35049:CDS | 40.0% |
| AACGCTGAATTTGAGGACGT+AGG | + | chr4.4:20493442-20493461 | None:intergenic | 45.0% | |
| ACGCTGAATTTGAGGACGTA+GGG | + | chr4.4:20493441-20493460 | None:intergenic | 45.0% | |
| GGCGCACAATAAACCAAAAG+AGG | + | chr4.4:20493419-20493438 | None:intergenic | 45.0% | |
| !! | AGTTGCTTTAGAGGATGTGG+TGG | - | chr4.4:20493327-20493346 | MS.gene35049:CDS | 45.0% |
| !! | TGCAGTTGCTTTAGAGGATG+TGG | - | chr4.4:20493324-20493343 | MS.gene35049:CDS | 45.0% |
| !!! | GTTTTGCGGGCTGCTTTTAA+AGG | - | chr4.4:20493663-20493682 | MS.gene35049:CDS | 45.0% |
| AACCATTGTTGGTGTGGCGT+CGG | + | chr4.4:20493296-20493315 | None:intergenic | 50.0% | |
| CGCTGAATTTGAGGACGTAG+GGG | + | chr4.4:20493440-20493459 | None:intergenic | 50.0% | |
| ! | AAGGGCCGTAGTAAGCATTG+TGG | + | chr4.4:20493245-20493264 | None:intergenic | 50.0% |
| ! | GGCTTCTGCAGTTGCTTTAG+AGG | - | chr4.4:20493318-20493337 | MS.gene35049:CDS | 50.0% |
| AGCCGCAACCATTGTTGGTG+TGG | + | chr4.4:20493302-20493321 | None:intergenic | 55.0% | |
| CGCCACACCAACAATGGTTG+CGG | - | chr4.4:20493297-20493316 | MS.gene35049:CDS | 55.0% | |
| GCAGAAGCCGCAACCATTGT+TGG | + | chr4.4:20493307-20493326 | None:intergenic | 55.0% | |
| ! | CGCTGGGCTTGTTTAGGTCT+AGG | - | chr4.4:20493793-20493812 | MS.gene35049:CDS | 55.0% |
| ! | GGTGTACGCTGGGCTTGTTT+AGG | - | chr4.4:20493787-20493806 | MS.gene35049:CDS | 55.0% |
| AGATTTCGACGGCGAGGGGA+TGG | + | chr4.4:20493268-20493287 | None:intergenic | 60.0% | |
| CGGTCAGATTTCGACGGCGA+GGG | + | chr4.4:20493273-20493292 | None:intergenic | 60.0% | |
| CGTCGGCGGTCAGATTTCGA+CGG | + | chr4.4:20493279-20493298 | None:intergenic | 60.0% | |
| GGTCAGATTTCGACGGCGAG+GGG | + | chr4.4:20493272-20493291 | None:intergenic | 60.0% | |
| ! | CATTGTTGGTGTGGCGTCGG+CGG | + | chr4.4:20493293-20493312 | None:intergenic | 60.0% |
| CGCCGACGCCACACCAACAA+TGG | - | chr4.4:20493291-20493310 | MS.gene35049:CDS | 65.0% | |
| GCGGTCAGATTTCGACGGCG+AGG | + | chr4.4:20493274-20493293 | None:intergenic | 65.0% | |
| TCGACGGCGAGGGGATGGAA+GGG | + | chr4.4:20493263-20493282 | None:intergenic | 65.0% | |
| TTCGACGGCGAGGGGATGGA+AGG | + | chr4.4:20493264-20493283 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 20493225 | 20493884 | 20493225 | ID=MS.gene35049 |
| chr4.4 | mRNA | 20493225 | 20493884 | 20493225 | ID=MS.gene35049.t1;Parent=MS.gene35049 |
| chr4.4 | exon | 20493225 | 20493884 | 20493225 | ID=MS.gene35049.t1.exon1;Parent=MS.gene35049.t1 |
| chr4.4 | CDS | 20493225 | 20493884 | 20493225 | ID=cds.MS.gene35049.t1;Parent=MS.gene35049.t1 |
| Gene Sequence |
| Protein sequence |
