AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41069


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41069.t1 MTR_1g057270 94.355 248 14 0 1 248 1 248 3.38e-147 411
MS.gene41069.t1 MTR_4g119428 63.415 246 87 1 1 246 2 244 1.78e-112 323
MS.gene41069.t1 MTR_7g105870 52.720 239 96 4 4 241 5 227 4.06e-61 192
MS.gene41069.t1 MTR_7g106000 40.562 249 118 6 1 242 1 226 8.05e-59 186
MS.gene41069.t1 MTR_7g106010 37.860 243 127 5 5 242 4 227 6.93e-48 158
MS.gene41069.t1 MTR_7g106030 39.912 228 114 5 5 227 7 216 2.01e-45 152
MS.gene41069.t1 MTR_1g057400 40.000 205 112 5 43 242 11 209 3.47e-45 150
MS.gene41069.t1 MTR_7g105970 39.419 241 119 5 5 241 8 225 6.09e-44 148
MS.gene41069.t1 MTR_6g071410 42.169 166 89 5 61 220 15 179 3.40e-33 120
MS.gene41069.t1 MTR_6g070890 42.169 166 89 5 61 220 15 179 3.40e-33 120
MS.gene41069.t1 MTR_6g471090 41.765 170 91 6 57 220 10 177 5.81e-32 117
MS.gene41069.t1 MTR_6g070900 36.970 165 99 3 61 220 15 179 1.28e-29 110
MS.gene41069.t1 MTR_6g071400 36.970 165 99 3 61 220 15 179 1.28e-29 110
MS.gene41069.t1 MTR_6g471080 43.860 171 86 7 57 220 7 174 4.25e-26 101
MS.gene41069.t1 MTR_1g057290 39.521 167 95 4 81 242 52 217 2.22e-24 97.1
MS.gene41069.t1 MTR_2g011350 31.469 143 97 1 73 215 40 181 5.86e-22 90.5
MS.gene41069.t1 MTR_4g094322 57.143 70 29 1 180 248 2 71 3.72e-21 84.3
MS.gene41069.t1 MTR_2g048270 40.816 147 80 6 95 238 2 144 2.25e-20 85.5
MS.gene41069.t1 MTR_3g063310 32.386 176 103 5 75 246 87 250 9.65e-18 80.1
MS.gene41069.t1 MTR_1g057230 50.000 66 32 1 181 246 30 94 1.18e-14 68.2
MS.gene41069.t1 MTR_5g076170 28.395 162 101 4 72 226 96 249 3.67e-14 70.5
MS.gene41069.t1 MTR_7g118270 27.711 166 116 3 58 221 47 210 5.21e-14 69.7
MS.gene41069.t1 MTR_3g078800 26.708 161 115 3 60 218 73 232 7.14e-14 69.7
MS.gene41069.t1 MTR_5g087760 26.697 221 132 8 15 225 41 241 9.56e-14 69.3
MS.gene41069.t1 MTR_2g100060 21.788 179 139 1 70 247 78 256 1.13e-12 66.2
MS.gene41069.t1 MTR_7g106050 26.630 184 114 4 55 230 23 193 2.73e-12 64.3
MS.gene41069.t1 MTR_3g078820 26.897 145 103 3 75 218 44 186 4.90e-12 63.9
MS.gene41069.t1 MTR_1g057300 40.000 100 53 3 43 142 12 104 9.35e-12 61.2
MS.gene41069.t1 MTR_1g105570 24.277 173 110 5 56 218 50 211 6.25e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41069.t1 AT3G11650 46.862 239 116 6 6 241 8 238 8.07e-71 217
MS.gene41069.t1 AT2G35980 44.490 245 115 8 1 242 1 227 8.54e-61 191
MS.gene41069.t1 AT2G35460 39.256 242 138 4 3 241 2 237 6.09e-60 189
MS.gene41069.t1 AT5G06320 42.857 238 125 3 6 242 4 231 1.09e-54 176
MS.gene41069.t1 AT1G32270 37.013 154 97 0 78 231 1 154 1.10e-27 108
MS.gene41069.t1 AT1G32270 37.013 154 97 0 78 231 1 154 3.51e-27 108
MS.gene41069.t1 AT5G22870 27.778 180 121 4 55 226 24 202 1.72e-18 81.3
MS.gene41069.t1 AT3G52470 29.143 175 122 2 57 230 19 192 6.29e-18 79.7
MS.gene41069.t1 AT4G01410 32.317 164 103 3 57 216 44 203 1.34e-17 79.3
MS.gene41069.t1 AT4G09590 29.341 167 102 3 57 215 22 180 3.65e-16 75.1
MS.gene41069.t1 AT4G05220 25.749 167 121 3 53 218 42 206 1.74e-15 73.6
MS.gene41069.t1 AT1G61760 25.455 165 118 4 56 218 43 204 8.06e-15 71.6
MS.gene41069.t1 AT5G53730 28.000 150 104 3 68 214 38 186 2.12e-14 70.5
MS.gene41069.t1 AT2G35970 28.743 167 103 3 57 215 22 180 2.38e-14 70.1
MS.gene41069.t1 AT3G11660 29.697 165 109 4 56 214 14 177 3.63e-13 67.0
MS.gene41069.t1 AT3G44220 25.000 168 124 2 57 223 20 186 5.72e-13 66.2
MS.gene41069.t1 AT2G35960 31.858 113 69 2 73 177 37 149 2.49e-12 64.7
MS.gene41069.t1 AT5G36970 24.026 154 108 4 69 218 78 226 2.55e-11 62.4
MS.gene41069.t1 AT3G52460 26.471 238 128 10 8 229 82 288 3.03e-11 62.8
MS.gene41069.t1 AT1G65690 26.733 101 72 2 58 157 71 170 7.36e-11 61.2

Find 61 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAAATTGATGGGGTTTA+TGG 0.105048 1.3:-42284769 MS.gene41069:CDS
ACATAACGGTTCTTCTTCTT+TGG 0.195709 1.3:-42284641 MS.gene41069:CDS
GGGATTACAAATGGAGATTA+TGG 0.235819 1.3:-42284588 MS.gene41069:CDS
TGATCCTCGGGTTCCTCTTC+TGG 0.237356 1.3:-42285101 MS.gene41069:CDS
TTTGAATGAAGATCAGAATA+AGG 0.294255 1.3:-42284803 MS.gene41069:CDS
TAAATACCAACTCTTCGATT+AGG 0.295908 1.3:+42284958 None:intergenic
TGCTGTGGAGGAGGGATTGA+TGG 0.300309 1.3:+42285270 None:intergenic
TTGAAATGAGGTTTAAATAT+TGG 0.313606 1.3:-42284726 MS.gene41069:CDS
CATAACGGTTCTTCTTCTTT+GGG 0.319706 1.3:-42284640 MS.gene41069:CDS
AAATACCAACTCTTCGATTA+GGG 0.324696 1.3:+42284959 None:intergenic
ACTTGTTTATCTTTGAAAAC+AGG 0.325553 1.3:+42284832 None:intergenic
AGCGCCGTTGAGTTGATTTC+CGG 0.331355 1.3:+42285302 None:intergenic
CGGTGAAATTGAATTGCGTT+AGG 0.356370 1.3:+42285031 None:intergenic
CAACTCAACGGCGCTTATTA+CGG 0.374153 1.3:-42285294 MS.gene41069:CDS
TTTGATAAGGAGAAAATTGA+TGG 0.386908 1.3:-42284778 MS.gene41069:CDS
ATCGTCATCCTCGTGATCCT+CGG 0.393582 1.3:-42285114 MS.gene41069:CDS
TTGAAGCTACTGAATGTGAT+TGG 0.408421 1.3:-42284609 MS.gene41069:CDS
TGTCTCTGCGGCTGCGTTCG+TGG 0.421661 1.3:-42285198 MS.gene41069:CDS
GGCTTCGGTTGCTGTGGAGG+AGG 0.424103 1.3:+42285261 None:intergenic
TCAGAATAAGGAGTTTGATA+AGG 0.436594 1.3:-42284791 MS.gene41069:CDS
TTGCGGTGTCGACGAGGCTT+CGG 0.436719 1.3:+42285246 None:intergenic
TAAGGTTCGTTGTGATTTGA+AGG 0.444006 1.3:-42284677 MS.gene41069:CDS
GGTTCTTCTTCTTTGGGGCA+TGG 0.470288 1.3:-42284634 MS.gene41069:CDS
GGTGAAATTGAATTGCGTTA+GGG 0.471511 1.3:+42285032 None:intergenic
CGTTAGGGTTGCATCGTTGA+CGG 0.471907 1.3:+42285047 None:intergenic
GTAGTGAAGTGTGTTGTTAT+CGG 0.474500 1.3:+42285011 None:intergenic
ACGGTGAATTTGACGACGTT+AGG 0.476132 1.3:+42285066 None:intergenic
TTCAAATCACAACGAACCTT+AGG 0.482759 1.3:+42284679 None:intergenic
TGAAGCTACTGAATGTGATT+GGG 0.483584 1.3:-42284608 MS.gene41069:CDS
CACCGCAACGACGACGGTTG+CGG 0.485935 1.3:-42285231 MS.gene41069:CDS
AATGTGATTGGGATTACAAA+TGG 0.489479 1.3:-42284597 MS.gene41069:CDS
CGAGGCTTCGGTTGCTGTGG+AGG 0.491545 1.3:+42285258 None:intergenic
GCTGTGGAGGAGGGATTGAT+GGG 0.498050 1.3:+42285271 None:intergenic
TCGTCATCCTCGTGATCCTC+GGG 0.502379 1.3:-42285113 MS.gene41069:CDS
CTTGTTTATCTTTGAAAACA+GGG 0.515714 1.3:+42284833 None:intergenic
TTAAGATGGTGATTGAAATG+AGG 0.522786 1.3:-42284738 MS.gene41069:CDS
ATAACGGTTCTTCTTCTTTG+GGG 0.527432 1.3:-42284639 MS.gene41069:CDS
GCTTCGGTTGCTGTGGAGGA+GGG 0.533543 1.3:+42285262 None:intergenic
GATTGGACATGCGAAACCTA+AGG 0.537152 1.3:-42284695 MS.gene41069:CDS
CAGTTTGCGAATCAAACCCT+AGG 0.540095 1.3:-42284895 MS.gene41069:CDS
TTGATAAGGAGAAAATTGAT+GGG 0.540563 1.3:-42284777 MS.gene41069:CDS
GGCTGCGTTCGTGGCTGTTG+CGG 0.543514 1.3:-42285189 MS.gene41069:CDS
CGTAACCCTAATCGAAGAGT+TGG 0.550944 1.3:-42284964 MS.gene41069:CDS
GATGCTGAAAGAATCTCCCT+AGG 0.552763 1.3:+42284878 None:intergenic
GAACCGTTATGTGATTTCAA+AGG 0.571830 1.3:+42284652 None:intergenic
CGACGAGGCTTCGGTTGCTG+TGG 0.573653 1.3:+42285255 None:intergenic
TGCCGCAACCGTCGTCGTTG+CGG 0.575842 1.3:+42285229 None:intergenic
CTATGACACAATTGAAACGA+TGG 0.582839 1.3:-42284935 MS.gene41069:CDS
ATGCTGAAAGAATCTCCCTA+GGG 0.603920 1.3:+42284879 None:intergenic
CGTCGACACCGCAACGACGA+CGG 0.610999 1.3:-42285237 MS.gene41069:CDS
GTGCCTTTGAAATCACATAA+CGG 0.619499 1.3:-42284655 MS.gene41069:CDS
TGATAAGGAGAAAATTGATG+GGG 0.622850 1.3:-42284776 MS.gene41069:CDS
GTCGAACAATGAGCCAGAAG+AGG 0.628717 1.3:+42285088 None:intergenic
CGATCTCGCTCTTAACGTCA+CGG 0.640093 1.3:-42284989 MS.gene41069:CDS
CACGAGGATGACGATGATTG+TGG 0.647428 1.3:+42285122 None:intergenic
AGTTTGCGAATCAAACCCTA+GGG 0.648044 1.3:-42284894 MS.gene41069:CDS
TGAGCCAGAAGAGGAACCCG+AGG 0.651046 1.3:+42285097 None:intergenic
AGAGGAACCCGAGGATCACG+AGG 0.656504 1.3:+42285106 None:intergenic
GGCATCTGCAGCTGTCTCTG+CGG 0.663821 1.3:-42285210 MS.gene41069:CDS
TCGTCGTTGCGGTGTCGACG+AGG 0.664976 1.3:+42285240 None:intergenic
ATGGCCGGAAATCAACTCAA+CGG 0.693203 1.3:-42285306 MS.gene41069:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTGGTTTTTTAAGAT+TGG - chr1.3:42285176-42285195 MS.gene41069:CDS 15.0%
!! TTGAAATGAGGTTTAAATAT+TGG - chr1.3:42285162-42285181 MS.gene41069:CDS 20.0%
!!! TTATGGAATTGATTTTAAGA+TGG - chr1.3:42285136-42285155 MS.gene41069:CDS 20.0%
! TTGATAAGGAGAAAATTGAT+GGG - chr1.3:42285111-42285130 MS.gene41069:CDS 25.0%
! TTTGAATGAAGATCAGAATA+AGG - chr1.3:42285085-42285104 MS.gene41069:CDS 25.0%
! TTTGATAAGGAGAAAATTGA+TGG - chr1.3:42285110-42285129 MS.gene41069:CDS 25.0%
!! ACTTGTTTATCTTTGAAAAC+AGG + chr1.3:42285059-42285078 None:intergenic 25.0%
!! CTTGTTTATCTTTGAAAACA+GGG + chr1.3:42285058-42285077 None:intergenic 25.0%
AAATACCAACTCTTCGATTA+GGG + chr1.3:42284932-42284951 None:intergenic 30.0%
AATGTGATTGGGATTACAAA+TGG - chr1.3:42285291-42285310 MS.gene41069:CDS 30.0%
TAAATACCAACTCTTCGATT+AGG + chr1.3:42284933-42284952 None:intergenic 30.0%
TCAGAATAAGGAGTTTGATA+AGG - chr1.3:42285097-42285116 MS.gene41069:CDS 30.0%
TGATAAGGAGAAAATTGATG+GGG - chr1.3:42285112-42285131 MS.gene41069:CDS 30.0%
TTAAGATGGTGATTGAAATG+AGG - chr1.3:42285150-42285169 MS.gene41069:CDS 30.0%
ACATAACGGTTCTTCTTCTT+TGG - chr1.3:42285247-42285266 MS.gene41069:CDS 35.0%
ATAACGGTTCTTCTTCTTTG+GGG - chr1.3:42285249-42285268 MS.gene41069:CDS 35.0%
CATAACGGTTCTTCTTCTTT+GGG - chr1.3:42285248-42285267 MS.gene41069:CDS 35.0%
CTATGACACAATTGAAACGA+TGG - chr1.3:42284953-42284972 MS.gene41069:CDS 35.0%
GAACCGTTATGTGATTTCAA+AGG + chr1.3:42285239-42285258 None:intergenic 35.0%
GAGAAAATTGATGGGGTTTA+TGG - chr1.3:42285119-42285138 MS.gene41069:CDS 35.0%
GGGATTACAAATGGAGATTA+TGG - chr1.3:42285300-42285319 MS.gene41069:CDS 35.0%
GGTGAAATTGAATTGCGTTA+GGG + chr1.3:42284859-42284878 None:intergenic 35.0%
TAAGGTTCGTTGTGATTTGA+AGG - chr1.3:42285211-42285230 MS.gene41069:CDS 35.0%
TGAAGCTACTGAATGTGATT+GGG - chr1.3:42285280-42285299 MS.gene41069:CDS 35.0%
TTCAAATCACAACGAACCTT+AGG + chr1.3:42285212-42285231 None:intergenic 35.0%
TTGAAGCTACTGAATGTGAT+TGG - chr1.3:42285279-42285298 MS.gene41069:CDS 35.0%
! GTAGTGAAGTGTGTTGTTAT+CGG + chr1.3:42284880-42284899 None:intergenic 35.0%
! TGAAACGATGGCGTTTTATA+AGG - chr1.3:42284965-42284984 MS.gene41069:CDS 35.0%
! TGCATTTTCAACTGCATCTT+AGG - chr1.3:42284723-42284742 MS.gene41069:CDS 35.0%
!! GTGCCTTTGAAATCACATAA+CGG - chr1.3:42285233-42285252 MS.gene41069:CDS 35.0%
AGTTTGCGAATCAAACCCTA+GGG - chr1.3:42284994-42285013 MS.gene41069:CDS 40.0%
ATGCTGAAAGAATCTCCCTA+GGG + chr1.3:42285012-42285031 None:intergenic 40.0%
CGGTGAAATTGAATTGCGTT+AGG + chr1.3:42284860-42284879 None:intergenic 40.0%
ACGGTGAATTTGACGACGTT+AGG + chr1.3:42284825-42284844 None:intergenic 45.0%
ATGGCCGGAAATCAACTCAA+CGG - chr1.3:42284582-42284601 MS.gene41069:CDS 45.0%
CAACTCAACGGCGCTTATTA+CGG - chr1.3:42284594-42284613 MS.gene41069:CDS 45.0%
CAGTTTGCGAATCAAACCCT+AGG - chr1.3:42284993-42285012 MS.gene41069:CDS 45.0%
CGTAACCCTAATCGAAGAGT+TGG - chr1.3:42284924-42284943 MS.gene41069:CDS 45.0%
GATGCTGAAAGAATCTCCCT+AGG + chr1.3:42285013-42285032 None:intergenic 45.0%
GATTGGACATGCGAAACCTA+AGG - chr1.3:42285193-42285212 MS.gene41069:CDS 45.0%
ATCGTCATCCTCGTGATCCT+CGG - chr1.3:42284774-42284793 MS.gene41069:CDS 50.0%
CACGAGGATGACGATGATTG+TGG + chr1.3:42284769-42284788 None:intergenic 50.0%
CGATCTCGCTCTTAACGTCA+CGG - chr1.3:42284899-42284918 MS.gene41069:CDS 50.0%
CGTTAGGGTTGCATCGTTGA+CGG + chr1.3:42284844-42284863 None:intergenic 50.0%
GTCGAACAATGAGCCAGAAG+AGG + chr1.3:42284803-42284822 None:intergenic 50.0%
!! AGCGCCGTTGAGTTGATTTC+CGG + chr1.3:42284589-42284608 None:intergenic 50.0%
!! GGTTCTTCTTCTTTGGGGCA+TGG - chr1.3:42285254-42285273 MS.gene41069:CDS 50.0%
GCTGTGGAGGAGGGATTGAT+GGG + chr1.3:42284620-42284639 None:intergenic 55.0%
TCGTCATCCTCGTGATCCTC+GGG - chr1.3:42284775-42284794 MS.gene41069:CDS 55.0%
TGATCCTCGGGTTCCTCTTC+TGG - chr1.3:42284787-42284806 MS.gene41069:CDS 55.0%
TGCTGTGGAGGAGGGATTGA+TGG + chr1.3:42284621-42284640 None:intergenic 55.0%
AGAGGAACCCGAGGATCACG+AGG + chr1.3:42284785-42284804 None:intergenic 60.0%
GCTTCGGTTGCTGTGGAGGA+GGG + chr1.3:42284629-42284648 None:intergenic 60.0%
GGCATCTGCAGCTGTCTCTG+CGG - chr1.3:42284678-42284697 MS.gene41069:CDS 60.0%
TGAGCCAGAAGAGGAACCCG+AGG + chr1.3:42284794-42284813 None:intergenic 60.0%
!! TTGCGGTGTCGACGAGGCTT+CGG + chr1.3:42284645-42284664 None:intergenic 60.0%
CACCGCAACGACGACGGTTG+CGG - chr1.3:42284657-42284676 MS.gene41069:CDS 65.0%
CGACGAGGCTTCGGTTGCTG+TGG + chr1.3:42284636-42284655 None:intergenic 65.0%
CGAGGCTTCGGTTGCTGTGG+AGG + chr1.3:42284633-42284652 None:intergenic 65.0%
CGTCGACACCGCAACGACGA+CGG - chr1.3:42284651-42284670 MS.gene41069:CDS 65.0%
GGCTGCGTTCGTGGCTGTTG+CGG - chr1.3:42284699-42284718 MS.gene41069:CDS 65.0%
GGCTTCGGTTGCTGTGGAGG+AGG + chr1.3:42284630-42284649 None:intergenic 65.0%
TGCCGCAACCGTCGTCGTTG+CGG + chr1.3:42284662-42284681 None:intergenic 65.0%
TGTCTCTGCGGCTGCGTTCG+TGG - chr1.3:42284690-42284709 MS.gene41069:CDS 65.0%
!! TCGTCGTTGCGGTGTCGACG+AGG + chr1.3:42284651-42284670 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 42284582 42285328 42284582 ID=MS.gene41069
chr1.3 mRNA 42284582 42285328 42284582 ID=MS.gene41069.t1;Parent=MS.gene41069
chr1.3 exon 42284582 42285328 42284582 ID=MS.gene41069.t1.exon1;Parent=MS.gene41069.t1
chr1.3 CDS 42284582 42285328 42284582 ID=cds.MS.gene41069.t1;Parent=MS.gene41069.t1
Gene Sequence

>MS.gene41069

ATGGCCGGAAATCAACTCAACGGCGCTTATTACGGCCCATCAATCCCTCCTCCACAGCAACCGAAGCCTCGTCGACACCGCAACGACGACGGTTGCGGCATCTGCAGCTGTCTCTGCGGCTGCGTTCGTGGCTGTTGCGGCTGCATTTTCAACTGCATCTTAGGCTTAATCTGCAAAATCATCACCACAATCATCGTCATCCTCGTGATCCTCGGGTTCCTCTTCTGGCTCATTGTTCGACCTAACGTCGTCAAATTCACCGTCAACGATGCAACCCTAACGCAATTCAATTTCACCGATAACAACACACTTCACTACGATCTCGCTCTTAACGTCACGGTTCGTAACCCTAATCGAAGAGTTGGTATTTACTATGACACAATTGAAACGATGGCGTTTTATAAGGATGTTCAGTTTGCGAATCAAACCCTAGGGAGATTCTTTCAGCATCATAAGAATACGAGTTTTTTGAACCCTGTTTTCAAAGATAAACAAGTTGTTGTTTTGAATGAAGATCAGAATAAGGAGTTTGATAAGGAGAAAATTGATGGGGTTTATGGAATTGATTTTAAGATGGTGATTGAAATGAGGTTTAAATATTGGTTTTTTAAGATTGGACATGCGAAACCTAAGGTTCGTTGTGATTTGAAGGTGCCTTTGAAATCACATAACGGTTCTTCTTCTTTGGGGCATGGTTTTGAAGCTACTGAATGTGATTGGGATTACAAATGGAGATTATGGCATTAG

Protein sequence

>MS.gene41069.t1

MAGNQLNGAYYGPSIPPPQQPKPRRHRNDDGCGICSCLCGCVRGCCGCIFNCILGLICKIITTIIVILVILGFLFWLIVRPNVVKFTVNDATLTQFNFTDNNTLHYDLALNVTVRNPNRRVGIYYDTIETMAFYKDVQFANQTLGRFFQHHKNTSFLNPVFKDKQVVVLNEDQNKEFDKEKIDGVYGIDFKMVIEMRFKYWFFKIGHAKPKVRCDLKVPLKSHNGSSSLGHGFEATECDWDYKWRLWH