Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36538.t1 | KEH16098.1 | 100 | 104 | 0 | 0 | 1 | 104 | 17 | 120 | 2.10E-53 | 218 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36538.t1 | Q558Z3 | 50.5 | 105 | 50 | 2 | 1 | 104 | 36 | 139 | 1.4e-26 | 120.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36538.t1 | A0A072TGI6 | 100.0 | 104 | 0 | 0 | 1 | 104 | 17 | 120 | 1.5e-53 | 218.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene36538 | -0.815618 | 8.43E-52 | -1.69E-46 |
| MS.gene049499 | MS.gene36538 | -0.823801 | 1.14E-53 | -1.69E-46 |
| MS.gene051699 | MS.gene36538 | -0.811391 | 7.17E-51 | -1.69E-46 |
| MS.gene051848 | MS.gene36538 | 0.801289 | 9.66E-49 | -1.69E-46 |
| MS.gene052534 | MS.gene36538 | -0.804388 | 2.21E-49 | -1.69E-46 |
| MS.gene052685 | MS.gene36538 | -0.821119 | 4.78E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene36538 | -0.821195 | 4.59E-53 | -1.69E-46 |
| MS.gene054222 | MS.gene36538 | -0.813263 | 2.80E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene36538 | -0.802 | 6.90E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene36538 | -0.818461 | 1.94E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene36538 | -0.817732 | 2.83E-52 | -1.69E-46 |
| MS.gene058159 | MS.gene36538 | -0.824228 | 9.04E-54 | -1.69E-46 |
| MS.gene058279 | MS.gene36538 | -0.818022 | 2.43E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene36538 | -0.809211 | 2.12E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene36538 | 0.834715 | 2.55E-56 | -1.69E-46 |
| MS.gene060935 | MS.gene36538 | -0.809138 | 2.19E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene36538 | -0.815624 | 8.40E-52 | -1.69E-46 |
| MS.gene061160 | MS.gene36538 | -0.804966 | 1.68E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36538.t1 | MTR_0332s0040 | 100.000 | 104 | 0 | 0 | 1 | 104 | 17 | 120 | 2.35e-72 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36538.t1 | AT1G73820 | 82.692 | 104 | 18 | 0 | 1 | 104 | 17 | 120 | 4.02e-63 | 190 |
| MS.gene36538.t1 | AT1G73820 | 82.692 | 104 | 18 | 0 | 1 | 104 | 33 | 136 | 5.28e-63 | 190 |
Find 20 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCCTGCCAATGCTTAAA+AGG | 0.252032 | 7.1:-7481054 | MS.gene36538:CDS |
| GAACCAAATGTCTATGAATT+TGG | 0.253761 | 7.1:-7482273 | MS.gene36538:CDS |
| GGGTTCGATGTATCTTCTTA+TGG | 0.256941 | 7.1:-7482336 | MS.gene36538:CDS |
| AAGGAATGCAGCTGTGAAAT+TGG | 0.321236 | 7.1:-7481035 | MS.gene36538:CDS |
| GTACCAAATTCATAGACATT+TGG | 0.395982 | 7.1:+7482270 | None:intergenic |
| GATGTATCTTCTTATGGAAC+AGG | 0.408399 | 7.1:-7482330 | MS.gene36538:CDS |
| GAGTGGAGCTACAAGCGAAA+TGG | 0.467833 | 7.1:-7481076 | MS.gene36538:intron |
| GTATTTGATATGGTTGTTGA+AGG | 0.475037 | 7.1:-7480944 | None:intergenic |
| TCATCAAGCATATATTTGTA+AGG | 0.512106 | 7.1:+7482246 | None:intergenic |
| TGAAATTGGCACCTCAAAGA+TGG | 0.526468 | 7.1:-7481021 | MS.gene36538:CDS |
| GGTTCTCTGAGGGAAGGACC+AGG | 0.532924 | 7.1:+7482291 | None:intergenic |
| GGTACCCACGTTAAGCTCCC+TGG | 0.567847 | 7.1:-7482309 | MS.gene36538:CDS |
| CATAGACATTTGGTTCTCTG+AGG | 0.569922 | 7.1:+7482280 | None:intergenic |
| ATAGACATTTGGTTCTCTGA+GGG | 0.571121 | 7.1:+7482281 | None:intergenic |
| AAGGACCAGGGAGCTTAACG+TGG | 0.600033 | 7.1:+7482304 | None:intergenic |
| ACATTTGGTTCTCTGAGGGA+AGG | 0.600394 | 7.1:+7482285 | None:intergenic |
| TGGCAAGAAAATGCTGCTGA+TGG | 0.614390 | 7.1:-7481001 | MS.gene36538:CDS |
| GTTCTCTGAGGGAAGGACCA+GGG | 0.632137 | 7.1:+7482292 | None:intergenic |
| TGCTGATGGCATATTTGATG+TGG | 0.692771 | 7.1:-7480987 | MS.gene36538:CDS |
| AGGACCAGGGAGCTTAACGT+GGG | 0.701054 | 7.1:+7482305 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAATTAAGTAAACAAA+AGG | + | chr7.1:7481146-7481165 | None:intergenic | 10.0% |
| !! | AAATAATTAAGTAAACAAAA+GGG | + | chr7.1:7481145-7481164 | None:intergenic | 10.0% |
| !! | TAAAAAATGCAAATAAAAAA+AGG | + | chr7.1:7481119-7481138 | None:intergenic | 10.0% |
| !!! | AAATTTAAGTTTTAGTTTTA+TGG | - | chr7.1:7481272-7481291 | MS.gene36538:intron | 10.0% |
| !!! | AATTTAAGTTTTAGTTTTAT+GGG | - | chr7.1:7481273-7481292 | MS.gene36538:intron | 10.0% |
| !!! | TACTTAATTATTTTAGTTTT+AGG | - | chr7.1:7481152-7481171 | MS.gene36538:intron | 10.0% |
| !!! | TTTTGTTTTAAAAGAATTTT+AGG | - | chr7.1:7481661-7481680 | MS.gene36538:intron | 10.0% |
| !! | AAAATGAAAATTTAGATTTG+GGG | - | chr7.1:7481590-7481609 | MS.gene36538:intron | 15.0% |
| !! | AAATTAAAAACAAGCAAAAT+TGG | + | chr7.1:7481321-7481340 | None:intergenic | 15.0% |
| !! | GAAAATGAAAATTTAGATTT+GGG | - | chr7.1:7481589-7481608 | MS.gene36538:intron | 15.0% |
| !! | TGAAAATGAAAATTTAGATT+TGG | - | chr7.1:7481588-7481607 | MS.gene36538:intron | 15.0% |
| !!! | ATTTAAGTTTTAGTTTTATG+GGG | - | chr7.1:7481274-7481293 | MS.gene36538:intron | 15.0% |
| !! | AAATGAAAATTTAGATTTGG+GGG | - | chr7.1:7481591-7481610 | MS.gene36538:intron | 20.0% |
| !! | TGATGATACTAAGAAATATT+GGG | - | chr7.1:7482083-7482102 | MS.gene36538:intron | 20.0% |
| !!! | AGATTTGTGTATTTTTCATA+AGG | - | chr7.1:7481399-7481418 | MS.gene36538:intron | 20.0% |
| !!! | ATTAACATTAATTTGTCTAC+AGG | - | chr7.1:7481511-7481530 | MS.gene36538:intron | 20.0% |
| !!! | TGAAGAAAAAGTATTTGATA+TGG | - | chr7.1:7482357-7482376 | MS.gene36538:CDS | 20.0% |
| !!! | TTTAGTTTTAGTAGAATTTC+AGG | - | chr7.1:7481343-7481362 | MS.gene36538:intron | 20.0% |
| ! | AATGAAAATTTAGATTTGGG+GGG | - | chr7.1:7481592-7481611 | MS.gene36538:intron | 25.0% |
| ! | ATAAAAAAAGGATACAGTTC+AGG | + | chr7.1:7481107-7481126 | None:intergenic | 25.0% |
| ! | ATTAATGTTAATATCGTGTC+TGG | + | chr7.1:7481503-7481522 | None:intergenic | 25.0% |
| ! | CTGATGATACTAAGAAATAT+TGG | - | chr7.1:7482082-7482101 | MS.gene36538:intron | 25.0% |
| ! | GATGATACTAAGAAATATTG+GGG | - | chr7.1:7482084-7482103 | MS.gene36538:intron | 25.0% |
| ! | TAAAAAAAGGATACAGTTCA+GGG | + | chr7.1:7481106-7481125 | None:intergenic | 25.0% |
| ! | TACAAAACAAGAATTCGAAA+TGG | + | chr7.1:7482189-7482208 | None:intergenic | 25.0% |
| ! | TAGGATTTAGTGAATAGTTT+GGG | - | chr7.1:7481749-7481768 | MS.gene36538:intron | 25.0% |
| ! | TCAGCTAAAAAAAATCGAAA+GGG | + | chr7.1:7481571-7481590 | None:intergenic | 25.0% |
| ! | TCATAAGGATTTATGATGAT+AGG | - | chr7.1:7481414-7481433 | MS.gene36538:intron | 25.0% |
| ! | TCATCAAGCATATATTTGTA+AGG | + | chr7.1:7481068-7481087 | None:intergenic | 25.0% |
| ! | TTAGGATTTAGTGAATAGTT+TGG | - | chr7.1:7481748-7481767 | MS.gene36538:intron | 25.0% |
| ! | TTCAGCTAAAAAAAATCGAA+AGG | + | chr7.1:7481572-7481591 | None:intergenic | 25.0% |
| ! | TTCAGCTAAAACAATCAAAA+GGG | + | chr7.1:7481254-7481273 | None:intergenic | 25.0% |
| ! | TTTCAGCTAAAACAATCAAA+AGG | + | chr7.1:7481255-7481274 | None:intergenic | 25.0% |
| !! | ACTTTCTTTTCAAAAGACAA+GGG | - | chr7.1:7480955-7480974 | MS.gene36538:CDS | 25.0% |
| !! | TGTATAATCTATGAAGTACT+GGG | - | chr7.1:7481782-7481801 | MS.gene36538:intron | 25.0% |
| !!! | TTTCGAATTCTTGTTTTGTA+TGG | - | chr7.1:7482189-7482208 | MS.gene36538:intron | 25.0% |
| !!! | TTTTTTCTTAAGCATAAGTG+AGG | - | chr7.1:7481175-7481194 | MS.gene36538:intron | 25.0% |
| AGGATTTATGATGATAGGTT+AGG | - | chr7.1:7481419-7481438 | MS.gene36538:intron | 30.0% | |
| GAACCAAATGTCTATGAATT+TGG | - | chr7.1:7481038-7481057 | MS.gene36538:CDS | 30.0% | |
| GAATTGTGTGTTTCTCATAA+AGG | - | chr7.1:7481710-7481729 | MS.gene36538:intron | 30.0% | |
| GTACCAAATTCATAGACATT+TGG | + | chr7.1:7481044-7481063 | None:intergenic | 30.0% | |
| GTGTATAATCTATGAAGTAC+TGG | - | chr7.1:7481781-7481800 | MS.gene36538:intron | 30.0% | |
| TAGGATTGAATGCATAGTAT+GGG | - | chr7.1:7481438-7481457 | MS.gene36538:intron | 30.0% | |
| TGAATAGTTTGGGAAAAAGA+TGG | - | chr7.1:7481759-7481778 | MS.gene36538:intron | 30.0% | |
| TGTTTCTCATAAAGGATTGA+GGG | - | chr7.1:7481718-7481737 | MS.gene36538:intron | 30.0% | |
| TTAGGATTGAATGCATAGTA+TGG | - | chr7.1:7481437-7481456 | MS.gene36538:intron | 30.0% | |
| ! | AATAATTGAGTTGTCAGTGT+CGG | - | chr7.1:7481871-7481890 | MS.gene36538:intron | 30.0% |
| ! | ATTTTGTAATTGAGGATGAG+GGG | - | chr7.1:7482107-7482126 | MS.gene36538:intron | 30.0% |
| ! | CACTTTCTTTTCAAAAGACA+AGG | - | chr7.1:7480954-7480973 | MS.gene36538:CDS | 30.0% |
| ! | GATTTTGTAATTGAGGATGA+GGG | - | chr7.1:7482106-7482125 | MS.gene36538:intron | 30.0% |
| ! | TACAGGAAGATGTGTATTTT+AGG | - | chr7.1:7481528-7481547 | MS.gene36538:intron | 30.0% |
| ! | TATTGGGGATTTTGTAATTG+AGG | - | chr7.1:7482099-7482118 | MS.gene36538:intron | 30.0% |
| !!! | TCTTGTTTTGTATGGTGAAA+TGG | - | chr7.1:7482197-7482216 | MS.gene36538:intron | 30.0% |
| ATCTGACACTGGTAGTAATT+TGG | - | chr7.1:7481819-7481838 | MS.gene36538:intron | 35.0% | |
| ATGCATAGTATGGGAAACAT+TGG | - | chr7.1:7481447-7481466 | MS.gene36538:intron | 35.0% | |
| CAAAATTGCCAGCATATCAA+AGG | + | chr7.1:7482047-7482066 | None:intergenic | 35.0% | |
| GATGTATCTTCTTATGGAAC+AGG | - | chr7.1:7480981-7481000 | MS.gene36538:CDS | 35.0% | |
| GTGTATAATCTACGAAGTTC+TGG | - | chr7.1:7481469-7481488 | MS.gene36538:intron | 35.0% | |
| GTGTTTCTCATAAAGGATTG+AGG | - | chr7.1:7481717-7481736 | MS.gene36538:intron | 35.0% | |
| GTTAATATCGTGTCTGGTAT+CGG | + | chr7.1:7481497-7481516 | None:intergenic | 35.0% | |
| TAGTAATTTGGGAATGACTC+TGG | - | chr7.1:7481831-7481850 | MS.gene36538:intron | 35.0% | |
| TCACTTTCGATCAGAAGTAA+CGG | - | chr7.1:7481903-7481922 | MS.gene36538:intron | 35.0% | |
| TCTGACACTGGTAGTAATTT+GGG | - | chr7.1:7481820-7481839 | MS.gene36538:intron | 35.0% | |
| ! | GGATTTTGTAATTGAGGATG+AGG | - | chr7.1:7482105-7482124 | MS.gene36538:intron | 35.0% |
| ! | TGATATGCTGGCAATTTTGT+TGG | - | chr7.1:7482048-7482067 | MS.gene36538:intron | 35.0% |
| !! | ATAGACATTTGGTTCTCTGA+GGG | + | chr7.1:7481033-7481052 | None:intergenic | 35.0% |
| AAGGAATGCAGCTGTGAAAT+TGG | - | chr7.1:7482276-7482295 | MS.gene36538:CDS | 40.0% | |
| CATAAAGGATTGAGGGTGAT+AGG | - | chr7.1:7481725-7481744 | MS.gene36538:intron | 40.0% | |
| CTTCTGATCGAAAGTGAATC+AGG | + | chr7.1:7481900-7481919 | None:intergenic | 40.0% | |
| GAATCCTGCCAATGCTTAAA+AGG | - | chr7.1:7482257-7482276 | MS.gene36538:CDS | 40.0% | |
| GGATGTATCCTTAACAAGAG+TGG | - | chr7.1:7482218-7482237 | MS.gene36538:intron | 40.0% | |
| GGGTTCGATGTATCTTCTTA+TGG | - | chr7.1:7480975-7480994 | MS.gene36538:CDS | 40.0% | |
| TAATTTGGGAATGACTCTGG+TGG | - | chr7.1:7481834-7481853 | MS.gene36538:intron | 40.0% | |
| TGAAATTGGCACCTCAAAGA+TGG | - | chr7.1:7482290-7482309 | MS.gene36538:CDS | 40.0% | |
| TGCTGATGGCATATTTGATG+TGG | - | chr7.1:7482324-7482343 | MS.gene36538:CDS | 40.0% | |
| TTCGAAATGGCATTTCCAAG+AGG | + | chr7.1:7482176-7482195 | None:intergenic | 40.0% | |
| TTGTAGCTCCACTCTTGTTA+AGG | + | chr7.1:7482229-7482248 | None:intergenic | 40.0% | |
| ! | GAGTGTTTTGCATCTGTTGT+TGG | - | chr7.1:7482149-7482168 | MS.gene36538:intron | 40.0% |
| ! | GCATTTTCTTGCCATCTTTG+AGG | + | chr7.1:7482304-7482323 | None:intergenic | 40.0% |
| ! | GCTGCATTCCTTTTAAGCAT+TGG | + | chr7.1:7482268-7482287 | None:intergenic | 40.0% |
| !! | CATAGACATTTGGTTCTCTG+AGG | + | chr7.1:7481034-7481053 | None:intergenic | 40.0% |
| !!! | CATTCCTTTTAAGCATTGGC+AGG | + | chr7.1:7482264-7482283 | None:intergenic | 40.0% |
| AGGATTGAGGGTGATAGGTT+AGG | - | chr7.1:7481730-7481749 | MS.gene36538:intron | 45.0% | |
| TACACTGGAACATCTGACAC+TGG | - | chr7.1:7481808-7481827 | MS.gene36538:intron | 45.0% | |
| TGAGGATGAGGGGTTAGATT+AGG | - | chr7.1:7482117-7482136 | MS.gene36538:intron | 45.0% | |
| TGGCAAGAAAATGCTGCTGA+TGG | - | chr7.1:7482310-7482329 | MS.gene36538:CDS | 45.0% | |
| ! | CCAGCATATCAAAGGCACAA+CGG | + | chr7.1:7482039-7482058 | None:intergenic | 45.0% |
| !! | ACATTTGGTTCTCTGAGGGA+AGG | + | chr7.1:7481029-7481048 | None:intergenic | 45.0% |
| !! | TGAAGTACTGGGACATACAC+TGG | - | chr7.1:7481793-7481812 | MS.gene36538:intron | 45.0% |
| CCGTTGTGCCTTTGATATGC+TGG | - | chr7.1:7482036-7482055 | MS.gene36538:intron | 50.0% | |
| GAGTGGAGCTACAAGCGAAA+TGG | - | chr7.1:7482235-7482254 | MS.gene36538:CDS | 50.0% | |
| GCATCTGTTGTTGGACCTCT+TGG | - | chr7.1:7482158-7482177 | MS.gene36538:intron | 50.0% | |
| AAGGACCAGGGAGCTTAACG+TGG | + | chr7.1:7481010-7481029 | None:intergenic | 55.0% | |
| AGGACCAGGGAGCTTAACGT+GGG | + | chr7.1:7481009-7481028 | None:intergenic | 55.0% | |
| ! | GTTCTCTGAGGGAAGGACCA+GGG | + | chr7.1:7481022-7481041 | None:intergenic | 55.0% |
| GGTACCCACGTTAAGCTCCC+TGG | - | chr7.1:7481002-7481021 | MS.gene36538:CDS | 60.0% | |
| ! | GGTTCTCTGAGGGAAGGACC+AGG | + | chr7.1:7481023-7481042 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 7480945 | 7482388 | 7480945 | ID=MS.gene36538 |
| chr7.1 | mRNA | 7480945 | 7482388 | 7480945 | ID=MS.gene36538.t1;Parent=MS.gene36538 |
| chr7.1 | exon | 7482222 | 7482388 | 7482222 | ID=MS.gene36538.t1.exon1;Parent=MS.gene36538.t1 |
| chr7.1 | CDS | 7482222 | 7482388 | 7482222 | ID=cds.MS.gene36538.t1;Parent=MS.gene36538.t1 |
| chr7.1 | exon | 7480945 | 7481090 | 7480945 | ID=MS.gene36538.t1.exon2;Parent=MS.gene36538.t1 |
| chr7.1 | CDS | 7480945 | 7481090 | 7480945 | ID=cds.MS.gene36538.t1;Parent=MS.gene36538.t1 |
| Gene Sequence |
| Protein sequence |
