Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37695.t1 | AAK63815.1 | 97 | 199 | 3 | 1 | 1 | 196 | 1 | 199 | 1.50E-81 | 312.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37695.t1 | P11432 | 74.9 | 199 | 44 | 2 | 1 | 196 | 1 | 196 | 3.0e-70 | 266.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37695.t1 | Q94F86 | 97.0 | 199 | 3 | 1 | 1 | 196 | 1 | 199 | 1.1e-81 | 312.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene37695 | 0.801186 | 1.01E-48 | -1.69E-46 |
| MS.gene05081 | MS.gene37695 | 0.818604 | 1.80E-52 | -1.69E-46 |
| MS.gene051734 | MS.gene37695 | 0.815627 | 8.39E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene37695 | 0.811814 | 5.80E-51 | -1.69E-46 |
| MS.gene052469 | MS.gene37695 | 0.845375 | 4.22E-59 | -1.69E-46 |
| MS.gene052870 | MS.gene37695 | 0.854329 | 1.32E-61 | -1.69E-46 |
| MS.gene052872 | MS.gene37695 | 0.849487 | 3.13E-60 | -1.69E-46 |
| MS.gene053824 | MS.gene37695 | 0.818907 | 1.53E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene37695 | 0.825591 | 4.31E-54 | -1.69E-46 |
| MS.gene055224 | MS.gene37695 | 0.818964 | 1.49E-52 | -1.69E-46 |
| MS.gene055539 | MS.gene37695 | 0.832054 | 1.18E-55 | -1.69E-46 |
| MS.gene055741 | MS.gene37695 | 0.811299 | 7.51E-51 | -1.69E-46 |
| MS.gene055744 | MS.gene37695 | 0.81049 | 1.12E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene37695 | 0.801274 | 9.73E-49 | -1.69E-46 |
| MS.gene058448 | MS.gene37695 | 0.803447 | 3.47E-49 | -1.69E-46 |
| MS.gene059717 | MS.gene37695 | 0.828997 | 6.59E-55 | -1.69E-46 |
| MS.gene060981 | MS.gene37695 | 0.801491 | 8.78E-49 | -1.69E-46 |
| MS.gene061154 | MS.gene37695 | 0.800763 | 1.24E-48 | -1.69E-46 |
| MS.gene061231 | MS.gene37695 | 0.81106 | 8.46E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37695.t1 | MTR_1g102780 | 95.000 | 200 | 6 | 2 | 1 | 196 | 1 | 200 | 5.15e-133 | 371 |
| MS.gene37695.t1 | MTR_1g102800 | 90.955 | 199 | 13 | 2 | 1 | 196 | 1 | 197 | 1.20e-106 | 304 |
| MS.gene37695.t1 | MTR_1g102570 | 86.111 | 180 | 20 | 2 | 20 | 196 | 21 | 198 | 8.40e-104 | 297 |
| MS.gene37695.t1 | MTR_1g102810 | 79.082 | 196 | 27 | 2 | 1 | 196 | 1 | 182 | 5.23e-96 | 277 |
| MS.gene37695.t1 | MTR_1g102830 | 79.444 | 180 | 20 | 3 | 20 | 196 | 21 | 186 | 6.55e-96 | 276 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37695.t1 | AT3G22840 | 54.774 | 199 | 83 | 5 | 1 | 196 | 1 | 195 | 7.76e-62 | 191 |
| MS.gene37695.t1 | AT4G14690 | 51.759 | 199 | 87 | 4 | 1 | 196 | 1 | 193 | 3.52e-57 | 179 |
Find 55 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGCTGATGGTGGTGTAAT+TGG | 0.245963 | 1.1:-73762777 | None:intergenic |
| TGGTGGAATTCCATGGTTCT+TGG | 0.288858 | 1.1:+73763087 | MS.gene37695:CDS |
| GGTGGAATTCCATGGTTCTT+GGG | 0.299911 | 1.1:+73763088 | MS.gene37695:CDS |
| GGTTTGTTTGATCAAATATC+TGG | 0.314167 | 1.1:+73763064 | MS.gene37695:CDS |
| GTTGGTTGTGGTTGTGGTGG+TGG | 0.344690 | 1.1:-73762804 | None:intergenic |
| TGTGGTTGTGGTGGTGGAGT+TGG | 0.352890 | 1.1:-73762798 | None:intergenic |
| TATGTTGGGTTTGATTGCTT+TGG | 0.357312 | 1.1:+73763228 | MS.gene37695:CDS |
| ATTGGGTCTACAGGCACCTT+TGG | 0.360861 | 1.1:-73762759 | None:intergenic |
| AATGGAAGGTTGGCTATGAT+TGG | 0.393839 | 1.1:+73763001 | MS.gene37695:CDS |
| CCATTGATTCTCTCAGGTGC+TGG | 0.411549 | 1.1:-73762983 | None:intergenic |
| CAAGCATTTCTTCTCAAGCT+TGG | 0.413181 | 1.1:-73762528 | None:intergenic |
| GGTGGTGGAGTTGGTGCTGA+TGG | 0.417357 | 1.1:-73762789 | None:intergenic |
| CACCTTTGGTGAACGAGTAT+AGG | 0.424707 | 1.1:-73762826 | None:intergenic |
| AACCTTCCATTGATTCTCTC+AGG | 0.436061 | 1.1:-73762989 | None:intergenic |
| GAATGGAAGAATAGCTATGT+TGG | 0.436992 | 1.1:+73763213 | MS.gene37695:CDS |
| GTGCTGATGGTGGTGTAATT+GGG | 0.440700 | 1.1:-73762776 | None:intergenic |
| GTATAGGTTGGTTGTGGTTG+TGG | 0.452125 | 1.1:-73762810 | None:intergenic |
| GTGGAATTCCATGGTTCTTG+GGG | 0.468120 | 1.1:+73763089 | MS.gene37695:CDS |
| GGTGGAGTTGGTGCTGATGG+TGG | 0.468618 | 1.1:-73762786 | None:intergenic |
| TGTCCTCAGATGCTGAGTTA+TGG | 0.479277 | 1.1:+73763191 | MS.gene37695:CDS |
| GAAATGCTTGCCTCAAAGTT+CGG | 0.480582 | 1.1:+73762541 | MS.gene37695:CDS |
| CGGATTTGATGGCATTTGGT+GGG | 0.485102 | 1.1:+73762960 | MS.gene37695:CDS |
| TCGGATTTGATGGCATTTGG+TGG | 0.487791 | 1.1:+73762959 | MS.gene37695:CDS |
| AATGGAAGAATAGCTATGTT+GGG | 0.501527 | 1.1:+73763214 | MS.gene37695:CDS |
| TCTAGGCCTGCTAGAAATGC+TGG | 0.515845 | 1.1:-73762478 | None:intergenic |
| GGTGGTGTAATTGGGTCTAC+AGG | 0.516474 | 1.1:-73762768 | None:intergenic |
| CCAGCACCTGAGAGAATCAA+TGG | 0.516939 | 1.1:+73762983 | MS.gene37695:CDS |
| CAGCCATGGACCGAACTTTG+AGG | 0.520058 | 1.1:-73762551 | None:intergenic |
| TAGGTTGGTTGTGGTTGTGG+TGG | 0.523841 | 1.1:-73762807 | None:intergenic |
| AGTGTGGAGTCTAAGTCAAA+AGG | 0.525000 | 1.1:+73763163 | MS.gene37695:CDS |
| CGAAAACATTACCTCAGCCA+TGG | 0.528935 | 1.1:-73762565 | None:intergenic |
| CAAAGTTCGGTCCATGGCTG+AGG | 0.533870 | 1.1:+73762554 | MS.gene37695:CDS |
| TTGCCTCAAAGTTCGGTCCA+TGG | 0.544126 | 1.1:+73762548 | MS.gene37695:CDS |
| GGTGGAGATAGCAAATGGAC+AGG | 0.547189 | 1.1:+73763042 | MS.gene37695:CDS |
| CCATGACCAGCATTTCTAGC+AGG | 0.549312 | 1.1:+73762472 | MS.gene37695:CDS |
| CCTGCTAGAAATGCTGGTCA+TGG | 0.550870 | 1.1:-73762472 | None:intergenic |
| TTTGATCAAATATCTGGTGG+TGG | 0.559193 | 1.1:+73763070 | MS.gene37695:CDS |
| TGAGAGAATCAATGGAAGGT+TGG | 0.560259 | 1.1:+73762991 | MS.gene37695:CDS |
| GAACGAGTATAGGTTGGTTG+TGG | 0.560878 | 1.1:-73762816 | None:intergenic |
| GAAGGTATGTTGTTAACTCT+AGG | 0.563861 | 1.1:-73762495 | None:intergenic |
| TCAGATGCTGAGTTATGGAA+TGG | 0.568279 | 1.1:+73763196 | MS.gene37695:CDS |
| TTGTTTGATCAAATATCTGG+TGG | 0.570609 | 1.1:+73763067 | MS.gene37695:CDS |
| CTATAGAAATCTATTACACA+AGG | 0.583097 | 1.1:-73763279 | None:intergenic |
| TTTGGTGAACGAGTATAGGT+TGG | 0.589396 | 1.1:-73762822 | None:intergenic |
| ATTCCATAACTCAGCATCTG+AGG | 0.591329 | 1.1:-73763194 | None:intergenic |
| AACCTATACTCGTTCACCAA+AGG | 0.597244 | 1.1:+73762824 | MS.gene37695:CDS |
| TAGACTCCACACTAACACCT+TGG | 0.597870 | 1.1:-73763153 | None:intergenic |
| TATCTGGTGGTGGAATTCCA+TGG | 0.624337 | 1.1:+73763080 | MS.gene37695:CDS |
| AAGCTTGGCATATACACAGA+AGG | 0.629885 | 1.1:-73762513 | None:intergenic |
| AGGGCAGAAAGAGCAACCAA+AGG | 0.630670 | 1.1:+73762743 | MS.gene37695:CDS |
| ACACTTGTCCCCAAGAACCA+TGG | 0.633615 | 1.1:-73763097 | None:intergenic |
| GTGGAGATAGCAAATGGACA+GGG | 0.645295 | 1.1:+73763043 | MS.gene37695:CDS |
| CACCTGAGAGAATCAATGGA+AGG | 0.646646 | 1.1:+73762987 | MS.gene37695:CDS |
| TATAGAAATCTATTACACAA+GGG | 0.705502 | 1.1:-73763278 | None:intergenic |
| AGAAATCTATTACACAAGGG+CGG | 0.719843 | 1.1:-73763275 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTAATAATGATGAAAATA+TGG | + | chr1.1:73762918-73762937 | MS.gene37695:intron | 15.0% |
| !!! | AGTTTTTCATTACATTTATC+AGG | + | chr1.1:73762719-73762738 | MS.gene37695:intron | 20.0% |
| ! | TTCATTACATTTATCAGGAA+GGG | + | chr1.1:73762724-73762743 | MS.gene37695:intron | 25.0% |
| ! | TTTCATTACATTTATCAGGA+AGG | + | chr1.1:73762723-73762742 | MS.gene37695:intron | 25.0% |
| !! | TGTTTTCGAAAACAGATTTA+AGG | + | chr1.1:73762580-73762599 | MS.gene37695:intron | 25.0% |
| AATGGAAGAATAGCTATGTT+GGG | + | chr1.1:73763214-73763233 | MS.gene37695:CDS | 30.0% | |
| TGCATAATGCAACAAGTTAA+TGG | + | chr1.1:73762634-73762653 | MS.gene37695:intron | 30.0% | |
| TTGTTTGATCAAATATCTGG+TGG | + | chr1.1:73763067-73763086 | MS.gene37695:CDS | 30.0% | |
| ! | GCTTTTACAGAGTATGTTAA+GGG | + | chr1.1:73763250-73763269 | MS.gene37695:CDS | 30.0% |
| ! | GGTTTGTTTGATCAAATATC+TGG | + | chr1.1:73763064-73763083 | MS.gene37695:CDS | 30.0% |
| !! | TCTTTGATTCCATTTTTCCA+AGG | + | chr1.1:73763136-73763155 | MS.gene37695:CDS | 30.0% |
| ACACTAACACCTTGGAAAAA+TGG | - | chr1.1:73763148-73763167 | None:intergenic | 35.0% | |
| GAATGGAAGAATAGCTATGT+TGG | + | chr1.1:73763213-73763232 | MS.gene37695:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | chr1.1:73763228-73763247 | MS.gene37695:CDS | 35.0% | |
| TTTACTACTATGCTCACCTT+TGG | - | chr1.1:73762843-73762862 | None:intergenic | 35.0% | |
| TTTGATCAAATATCTGGTGG+TGG | + | chr1.1:73763070-73763089 | MS.gene37695:CDS | 35.0% | |
| ! | CACAAAGTTTTCGGATTTGA+TGG | + | chr1.1:73762949-73762968 | MS.gene37695:CDS | 35.0% |
| ! | GAAGGTATGTTGTTAACTCT+AGG | - | chr1.1:73762498-73762517 | None:intergenic | 35.0% |
| ! | GGCTTTTACAGAGTATGTTA+AGG | + | chr1.1:73763249-73763268 | MS.gene37695:CDS | 35.0% |
| !! | ATTTTTCCAAGGTGTTAGTG+TGG | + | chr1.1:73763147-73763166 | MS.gene37695:CDS | 35.0% |
| !! | TTTTCGGATTTGATGGCATT+TGG | + | chr1.1:73762956-73762975 | MS.gene37695:CDS | 35.0% |
| !!! | ATTGGTTTTGTAGCTGCTAT+GGG | + | chr1.1:73763019-73763038 | MS.gene37695:CDS | 35.0% |
| AACCTATACTCGTTCACCAA+AGG | + | chr1.1:73762824-73762843 | MS.gene37695:CDS | 40.0% | |
| AAGCTTGGCATATACACAGA+AGG | - | chr1.1:73762516-73762535 | None:intergenic | 40.0% | |
| AGTGTGGAGTCTAAGTCAAA+AGG | + | chr1.1:73763163-73763182 | MS.gene37695:CDS | 40.0% | |
| ATTCCATAACTCAGCATCTG+AGG | - | chr1.1:73763197-73763216 | None:intergenic | 40.0% | |
| CAAGCATTTCTTCTCAAGCT+TGG | - | chr1.1:73762531-73762550 | None:intergenic | 40.0% | |
| GAAATGCTTGCCTCAAAGTT+CGG | + | chr1.1:73762541-73762560 | MS.gene37695:CDS | 40.0% | |
| TCAGATGCTGAGTTATGGAA+TGG | + | chr1.1:73763196-73763215 | MS.gene37695:CDS | 40.0% | |
| TGAGAGAATCAATGGAAGGT+TGG | + | chr1.1:73762991-73763010 | MS.gene37695:CDS | 40.0% | |
| TTTGGTGAACGAGTATAGGT+TGG | - | chr1.1:73762825-73762844 | None:intergenic | 40.0% | |
| ! | AATGGAAGGTTGGCTATGAT+TGG | + | chr1.1:73763001-73763020 | MS.gene37695:CDS | 40.0% |
| ! | GCAGATGAGCACAAAGTTTT+CGG | + | chr1.1:73762940-73762959 | MS.gene37695:intron | 40.0% |
| !! | AACCTTCCATTGATTCTCTC+AGG | - | chr1.1:73762992-73763011 | None:intergenic | 40.0% |
| !!! | GATTGGTTTTGTAGCTGCTA+TGG | + | chr1.1:73763018-73763037 | MS.gene37695:CDS | 40.0% |
| !!! | TTGGTTTTGTAGCTGCTATG+GGG | + | chr1.1:73763020-73763039 | MS.gene37695:CDS | 40.0% |
| CACCTGAGAGAATCAATGGA+AGG | + | chr1.1:73762987-73763006 | MS.gene37695:CDS | 45.0% | |
| CACCTTTGGTGAACGAGTAT+AGG | - | chr1.1:73762829-73762848 | None:intergenic | 45.0% | |
| CGAAAACATTACCTCAGCCA+TGG | - | chr1.1:73762568-73762587 | None:intergenic | 45.0% | |
| GGTGGAATTCCATGGTTCTT+GGG | + | chr1.1:73763088-73763107 | MS.gene37695:CDS | 45.0% | |
| GTGGAATTCCATGGTTCTTG+GGG | + | chr1.1:73763089-73763108 | MS.gene37695:CDS | 45.0% | |
| GTGGAGATAGCAAATGGACA+GGG | + | chr1.1:73763043-73763062 | MS.gene37695:CDS | 45.0% | |
| TAGACTCCACACTAACACCT+TGG | - | chr1.1:73763156-73763175 | None:intergenic | 45.0% | |
| TGGTGGAATTCCATGGTTCT+TGG | + | chr1.1:73763087-73763106 | MS.gene37695:CDS | 45.0% | |
| TGTCCTCAGATGCTGAGTTA+TGG | + | chr1.1:73763191-73763210 | MS.gene37695:CDS | 45.0% | |
| ! | CGGATTTGATGGCATTTGGT+GGG | + | chr1.1:73762960-73762979 | MS.gene37695:CDS | 45.0% |
| ! | TCGGATTTGATGGCATTTGG+TGG | + | chr1.1:73762959-73762978 | MS.gene37695:CDS | 45.0% |
| !! | GAACGAGTATAGGTTGGTTG+TGG | - | chr1.1:73762819-73762838 | None:intergenic | 45.0% |
| !! | GTATAGGTTGGTTGTGGTTG+TGG | - | chr1.1:73762813-73762832 | None:intergenic | 45.0% |
| !! | GTGCTGATGGTGGTGTAATT+GGG | - | chr1.1:73762779-73762798 | None:intergenic | 45.0% |
| !! | TATCTGGTGGTGGAATTCCA+TGG | + | chr1.1:73763080-73763099 | MS.gene37695:CDS | 45.0% |
| !!! | TGGTTTTGTAGCTGCTATGG+GGG | + | chr1.1:73763021-73763040 | MS.gene37695:CDS | 45.0% |
| ACACTTGTCCCCAAGAACCA+TGG | - | chr1.1:73763100-73763119 | None:intergenic | 50.0% | |
| AGGGCAGAAAGAGCAACCAA+AGG | + | chr1.1:73762743-73762762 | MS.gene37695:CDS | 50.0% | |
| ATGGGGGTGGAGATAGCAAA+TGG | + | chr1.1:73763037-73763056 | MS.gene37695:CDS | 50.0% | |
| ATTGGGTCTACAGGCACCTT+TGG | - | chr1.1:73762762-73762781 | None:intergenic | 50.0% | |
| CCAGCACCTGAGAGAATCAA+TGG | + | chr1.1:73762983-73763002 | MS.gene37695:CDS | 50.0% | |
| CCTGCTAGAAATGCTGGTCA+TGG | - | chr1.1:73762475-73762494 | None:intergenic | 50.0% | |
| GGTGGAGATAGCAAATGGAC+AGG | + | chr1.1:73763042-73763061 | MS.gene37695:CDS | 50.0% | |
| GGTGGTGTAATTGGGTCTAC+AGG | - | chr1.1:73762771-73762790 | None:intergenic | 50.0% | |
| TCTAGGCCTGCTAGAAATGC+TGG | - | chr1.1:73762481-73762500 | None:intergenic | 50.0% | |
| TTGCCTCAAAGTTCGGTCCA+TGG | + | chr1.1:73762548-73762567 | MS.gene37695:CDS | 50.0% | |
| ! | TTTTGTAGCTGCTATGGGGG+TGG | + | chr1.1:73763024-73763043 | MS.gene37695:CDS | 50.0% |
| !! | CCATGACCAGCATTTCTAGC+AGG | + | chr1.1:73762472-73762491 | MS.gene37695:CDS | 50.0% |
| !! | CCATTGATTCTCTCAGGTGC+TGG | - | chr1.1:73762986-73763005 | None:intergenic | 50.0% |
| !! | GGTGCTGATGGTGGTGTAAT+TGG | - | chr1.1:73762780-73762799 | None:intergenic | 50.0% |
| !! | TAGGTTGGTTGTGGTTGTGG+TGG | - | chr1.1:73762810-73762829 | None:intergenic | 50.0% |
| CAAAGTTCGGTCCATGGCTG+AGG | + | chr1.1:73762554-73762573 | MS.gene37695:CDS | 55.0% | |
| CAGCCATGGACCGAACTTTG+AGG | - | chr1.1:73762554-73762573 | None:intergenic | 55.0% | |
| GTTGGTTGTGGTTGTGGTGG+TGG | - | chr1.1:73762807-73762826 | None:intergenic | 55.0% | |
| !! | TGTGGTTGTGGTGGTGGAGT+TGG | - | chr1.1:73762801-73762820 | None:intergenic | 55.0% |
| !! | GGTGGAGTTGGTGCTGATGG+TGG | - | chr1.1:73762789-73762808 | None:intergenic | 60.0% |
| !! | GGTGGTGGAGTTGGTGCTGA+TGG | - | chr1.1:73762792-73762811 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 73762435 | 73763288 | 73762435 | ID=MS.gene37695 |
| chr1.1 | mRNA | 73762435 | 73763288 | 73762435 | ID=MS.gene37695.t1;Parent=MS.gene37695 |
| chr1.1 | exon | 73762435 | 73762575 | 73762435 | ID=MS.gene37695.t1.exon1;Parent=MS.gene37695.t1 |
| chr1.1 | CDS | 73762435 | 73762575 | 73762435 | ID=cds.MS.gene37695.t1;Parent=MS.gene37695.t1 |
| chr1.1 | exon | 73762741 | 73762845 | 73762741 | ID=MS.gene37695.t1.exon2;Parent=MS.gene37695.t1 |
| chr1.1 | CDS | 73762741 | 73762845 | 73762741 | ID=cds.MS.gene37695.t1;Parent=MS.gene37695.t1 |
| chr1.1 | exon | 73762944 | 73763288 | 73762944 | ID=MS.gene37695.t1.exon3;Parent=MS.gene37695.t1 |
| chr1.1 | CDS | 73762944 | 73763288 | 73762944 | ID=cds.MS.gene37695.t1;Parent=MS.gene37695.t1 |
| Gene Sequence |
| Protein sequence |
