Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38338.t1 | XP_003622535.1 | 89 | 145 | 16 | 0 | 1 | 145 | 1 | 145 | 1.20E-70 | 275.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38338.t1 | P62927 | 42.7 | 131 | 69 | 4 | 1 | 128 | 1 | 128 | 5.2e-19 | 95.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38338.t1 | G7KTY0 | 89.0 | 145 | 16 | 0 | 1 | 145 | 1 | 145 | 8.3e-71 | 275.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052223 | MS.gene38338 | 0.812065 | 5.11E-51 | -1.69E-46 |
| MS.gene054742 | MS.gene38338 | 0.802288 | 6.02E-49 | -1.69E-46 |
| MS.gene055595 | MS.gene38338 | 0.815938 | 7.15E-52 | -1.69E-46 |
| MS.gene055597 | MS.gene38338 | 0.810306 | 1.23E-50 | -1.69E-46 |
| MS.gene056690 | MS.gene38338 | 0.805801 | 1.12E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene38338 | 0.808647 | 2.79E-50 | -1.69E-46 |
| MS.gene056920 | MS.gene38338 | 0.826191 | 3.11E-54 | -1.69E-46 |
| MS.gene056923 | MS.gene38338 | 0.819142 | 1.36E-52 | -1.69E-46 |
| MS.gene056924 | MS.gene38338 | 0.810457 | 1.14E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene38338 | 0.807195 | 5.69E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38338.t1 | MTR_7g044980 | 88.966 | 145 | 16 | 0 | 1 | 145 | 1 | 145 | 2.73e-94 | 269 |
| MS.gene38338.t1 | MTR_7g044920 | 57.895 | 152 | 57 | 3 | 1 | 145 | 1 | 152 | 5.73e-55 | 169 |
| MS.gene38338.t1 | MTR_8g056800 | 69.792 | 96 | 28 | 1 | 50 | 145 | 7 | 101 | 1.49e-43 | 139 |
| MS.gene38338.t1 | MTR_3g436100 | 45.161 | 155 | 74 | 6 | 1 | 145 | 1 | 154 | 1.54e-36 | 123 |
| MS.gene38338.t1 | MTR_8g022430 | 48.837 | 129 | 58 | 5 | 1 | 124 | 1 | 126 | 4.46e-34 | 116 |
| MS.gene38338.t1 | MTR_6g017170 | 54.032 | 124 | 49 | 6 | 1 | 117 | 1 | 123 | 4.48e-34 | 116 |
| MS.gene38338.t1 | MTR_8g022400 | 50.382 | 131 | 59 | 4 | 1 | 125 | 1 | 131 | 1.37e-33 | 115 |
| MS.gene38338.t1 | MTR_8g022420 | 51.145 | 131 | 57 | 6 | 1 | 125 | 1 | 130 | 7.36e-33 | 113 |
| MS.gene38338.t1 | MTR_6g017150 | 51.515 | 132 | 57 | 6 | 1 | 125 | 1 | 132 | 1.73e-32 | 112 |
| MS.gene38338.t1 | MTR_3g067270 | 47.287 | 129 | 64 | 3 | 1 | 125 | 1 | 129 | 5.21e-32 | 111 |
| MS.gene38338.t1 | MTR_3g467750 | 47.287 | 129 | 64 | 3 | 1 | 125 | 1 | 129 | 5.21e-32 | 111 |
| MS.gene38338.t1 | MTR_3g463570 | 48.855 | 131 | 61 | 5 | 1 | 125 | 1 | 131 | 1.26e-31 | 110 |
| MS.gene38338.t1 | MTR_8g461020 | 45.865 | 133 | 64 | 4 | 3 | 128 | 4 | 135 | 6.55e-31 | 108 |
| MS.gene38338.t1 | MTR_3g067280 | 46.512 | 129 | 65 | 3 | 1 | 125 | 1 | 129 | 1.05e-30 | 107 |
| MS.gene38338.t1 | MTR_3g067437 | 47.287 | 129 | 63 | 3 | 1 | 124 | 1 | 129 | 1.48e-30 | 107 |
| MS.gene38338.t1 | MTR_6g036620 | 45.517 | 145 | 62 | 6 | 1 | 141 | 1 | 132 | 4.22e-29 | 103 |
| MS.gene38338.t1 | MTR_7g056817 | 47.015 | 134 | 62 | 5 | 1 | 125 | 1 | 134 | 7.24e-28 | 100 |
| MS.gene38338.t1 | MTR_4g026590 | 42.038 | 157 | 67 | 5 | 1 | 145 | 1 | 145 | 3.77e-27 | 99.0 |
| MS.gene38338.t1 | MTR_3g463570 | 43.307 | 127 | 68 | 3 | 3 | 125 | 25 | 151 | 2.09e-26 | 97.4 |
| MS.gene38338.t1 | MTR_6g047880 | 43.182 | 132 | 66 | 6 | 1 | 124 | 1 | 131 | 3.02e-26 | 96.7 |
| MS.gene38338.t1 | MTR_6g047900 | 45.865 | 133 | 60 | 7 | 1 | 125 | 1 | 129 | 1.40e-25 | 94.7 |
| MS.gene38338.t1 | MTR_3g067510 | 46.218 | 119 | 50 | 4 | 1 | 109 | 1 | 115 | 2.25e-24 | 91.7 |
| MS.gene38338.t1 | MTR_7g056803 | 46.617 | 133 | 63 | 6 | 1 | 125 | 1 | 133 | 2.47e-24 | 91.7 |
| MS.gene38338.t1 | MTR_3g067570 | 48.913 | 92 | 42 | 2 | 21 | 109 | 1 | 90 | 3.47e-24 | 89.7 |
| MS.gene38338.t1 | MTR_3g067430 | 40.000 | 135 | 71 | 4 | 1 | 125 | 1 | 135 | 6.96e-24 | 90.5 |
| MS.gene38338.t1 | MTR_3g467760 | 41.732 | 127 | 50 | 4 | 1 | 125 | 1 | 105 | 9.81e-24 | 89.4 |
| MS.gene38338.t1 | MTR_3g067510 | 44.355 | 124 | 50 | 4 | 1 | 109 | 1 | 120 | 1.80e-23 | 89.4 |
| MS.gene38338.t1 | MTR_3g067500 | 39.823 | 113 | 65 | 2 | 21 | 130 | 1 | 113 | 9.36e-23 | 87.0 |
| MS.gene38338.t1 | MTR_3g067445 | 42.735 | 117 | 58 | 3 | 1 | 108 | 1 | 117 | 3.64e-22 | 85.5 |
| MS.gene38338.t1 | MTR_3g067580 | 40.458 | 131 | 72 | 4 | 1 | 125 | 9 | 139 | 5.21e-22 | 85.9 |
| MS.gene38338.t1 | MTR_3g067540 | 42.647 | 136 | 66 | 7 | 1 | 125 | 1 | 135 | 1.70e-21 | 84.3 |
| MS.gene38338.t1 | MTR_3g067555 | 42.647 | 136 | 66 | 7 | 1 | 125 | 1 | 135 | 1.70e-21 | 84.3 |
| MS.gene38338.t1 | MTR_1g019650 | 43.966 | 116 | 54 | 6 | 1 | 114 | 1 | 107 | 1.61e-20 | 80.9 |
| MS.gene38338.t1 | MTR_7g029540 | 39.535 | 129 | 54 | 6 | 1 | 114 | 1 | 120 | 1.06e-19 | 79.3 |
| MS.gene38338.t1 | MTR_3g067535 | 43.382 | 136 | 61 | 9 | 1 | 125 | 1 | 131 | 3.36e-19 | 78.6 |
| MS.gene38338.t1 | MTR_3g067555 | 39.024 | 123 | 64 | 6 | 13 | 125 | 1 | 122 | 1.74e-16 | 71.2 |
| MS.gene38338.t1 | MTR_3g067550 | 39.130 | 115 | 59 | 6 | 21 | 125 | 1 | 114 | 1.59e-14 | 65.9 |
| MS.gene38338.t1 | MTR_3g067555 | 39.130 | 115 | 59 | 6 | 21 | 125 | 1 | 114 | 1.59e-14 | 65.9 |
| MS.gene38338.t1 | MTR_6g082060 | 35.484 | 155 | 77 | 6 | 1 | 141 | 1 | 146 | 2.65e-12 | 60.8 |
| MS.gene38338.t1 | MTR_3g067830 | 40.000 | 105 | 51 | 5 | 1 | 95 | 1 | 103 | 3.02e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGAATCCATTTCCATTAA+TGG | 0.138065 | 7.3:-57419674 | None:intergenic |
| CCATGTTCGATTTCAGAATT+AGG | 0.219721 | 7.3:-57419559 | None:intergenic |
| AAGAATCCATTTCCATTAAT+GGG | 0.254802 | 7.3:-57419673 | None:intergenic |
| ACTGTATCTTACCCCATTAA+TGG | 0.304225 | 7.3:+57419661 | MS.gene38338:CDS |
| GGCTTATGTTAAGCTTGCTT+TGG | 0.336285 | 7.3:+57418960 | MS.gene38338:CDS |
| TCTTACCCCATTAATGGAAA+TGG | 0.347113 | 7.3:+57419667 | MS.gene38338:CDS |
| TGCCTCGCGTGGGGAATAAT+TGG | 0.352167 | 7.3:+57419421 | MS.gene38338:CDS |
| GATTGCATCAAGAAGGGAAA+TGG | 0.379888 | 7.3:+57419517 | MS.gene38338:CDS |
| GCGTTCGGCTTCAACGTTAG+AGG | 0.426857 | 7.3:-57419593 | None:intergenic |
| CCACGCGAGGCAACCACATC+CGG | 0.431307 | 7.3:-57419410 | None:intergenic |
| AATGTGGGATTGACATAAGT+TGG | 0.435348 | 7.3:-57419494 | None:intergenic |
| CATGCTTCTACCTTCTTCAT+CGG | 0.451053 | 7.3:-57419343 | None:intergenic |
| ATGCTTCTACCTTCTTCATC+GGG | 0.462775 | 7.3:-57419342 | None:intergenic |
| GATTAAGTGGCAATCTCTAC+AGG | 0.474451 | 7.3:-57419703 | None:intergenic |
| CCGGATGTGGTTGCCTCGCG+TGG | 0.488380 | 7.3:+57419410 | MS.gene38338:CDS |
| CTTCTTGATGCAATCAATGT+GGG | 0.499847 | 7.3:-57419509 | None:intergenic |
| ATTGCATCAAGAAGGGAAAT+GGG | 0.503880 | 7.3:+57419518 | MS.gene38338:CDS |
| ATAGACATATCAAGATTAAG+TGG | 0.519324 | 7.3:-57419716 | None:intergenic |
| ACAGTATGTGGCTCCGGATG+TGG | 0.521366 | 7.3:+57419397 | MS.gene38338:CDS |
| CGGATGTGGTTGCCTCGCGT+GGG | 0.521966 | 7.3:+57419411 | MS.gene38338:CDS |
| TCAGTTCATACAACAGTATG+TGG | 0.525112 | 7.3:+57419385 | MS.gene38338:CDS |
| CCACATTGATTGCATCAAGA+AGG | 0.531789 | 7.3:+57419510 | MS.gene38338:CDS |
| CATACAACAGTATGTGGCTC+CGG | 0.542165 | 7.3:+57419391 | MS.gene38338:CDS |
| ACGACCATCTCTTACGAAGA+TGG | 0.542973 | 7.3:+57419459 | MS.gene38338:CDS |
| GAGAATCTCGAAGTAGCGTT+CGG | 0.552820 | 7.3:-57419608 | None:intergenic |
| TCGACCATCTTCGTAAGAGA+TGG | 0.555256 | 7.3:-57419463 | None:intergenic |
| AGTCATAAGCCCGATGAAGA+AGG | 0.556957 | 7.3:+57419333 | MS.gene38338:intron |
| CTCGCGTGGGGAATAATTGG+TGG | 0.561996 | 7.3:+57419424 | MS.gene38338:CDS |
| ATGTGGGATTGACATAAGTT+GGG | 0.564035 | 7.3:-57419493 | None:intergenic |
| CCTAATTCTGAAATCGAACA+TGG | 0.569253 | 7.3:+57419559 | MS.gene38338:CDS |
| CACATTGATTGCATCAAGAA+GGG | 0.569807 | 7.3:+57419511 | MS.gene38338:CDS |
| ATTCTGAAATCGAACATGGC+TGG | 0.571433 | 7.3:+57419563 | MS.gene38338:CDS |
| CCTTCTTGATGCAATCAATG+TGG | 0.579394 | 7.3:-57419510 | None:intergenic |
| AGAATCCATTTCCATTAATG+GGG | 0.584366 | 7.3:-57419672 | None:intergenic |
| GGATGTGGTTGCCTCGCGTG+GGG | 0.586874 | 7.3:+57419412 | MS.gene38338:CDS |
| TAGAAGCATGTGACACAACA+TGG | 0.603510 | 7.3:+57419356 | MS.gene38338:CDS |
| CACCAATTATTCCCCACGCG+AGG | 0.633688 | 7.3:-57419423 | None:intergenic |
| ACAAATTGTTGCAAGAAAGA+CGG | 0.634388 | 7.3:-57418986 | None:intergenic |
| TGGGGTAAGATACAGTCTTG+AGG | 0.662338 | 7.3:-57419654 | None:intergenic |
| ATCTTCGTAAGAGATGGTCG+TGG | 0.691138 | 7.3:-57419457 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAATGCATGGTATATAA+AGG | - | chr7.3:57419036-57419055 | None:intergenic | 20.0% |
| !! | CTAACATAAAAATAAATGCA+TGG | - | chr7.3:57419046-57419065 | None:intergenic | 20.0% |
| ! | AAGAATCCATTTCCATTAAT+GGG | - | chr7.3:57419676-57419695 | None:intergenic | 25.0% |
| ! | ACTAAGTATGTAACTTATTC+AGG | + | chr7.3:57419194-57419213 | MS.gene38338:intron | 25.0% |
| !! | CATTAATTTAGAGTTGATTC+TGG | - | chr7.3:57419088-57419107 | None:intergenic | 25.0% |
| !! | TTTTATGTTAGAAAACTCGT+GGG | + | chr7.3:57419055-57419074 | MS.gene38338:intron | 25.0% |
| !! | TTTTTATGTTAGAAAACTCG+TGG | + | chr7.3:57419054-57419073 | MS.gene38338:intron | 25.0% |
| ACAAATTGTTGCAAGAAAGA+CGG | - | chr7.3:57418989-57419008 | None:intergenic | 30.0% | |
| AGAATCCATTTCCATTAATG+GGG | - | chr7.3:57419675-57419694 | None:intergenic | 30.0% | |
| CAAGAATCCATTTCCATTAA+TGG | - | chr7.3:57419677-57419696 | None:intergenic | 30.0% | |
| AATGTGGGATTGACATAAGT+TGG | - | chr7.3:57419497-57419516 | None:intergenic | 35.0% | |
| ACTGTATCTTACCCCATTAA+TGG | + | chr7.3:57419661-57419680 | MS.gene38338:CDS | 35.0% | |
| ATGGGAAAGTCAGTTAAAGT+TGG | - | chr7.3:57419309-57419328 | None:intergenic | 35.0% | |
| ATGTGGGATTGACATAAGTT+GGG | - | chr7.3:57419496-57419515 | None:intergenic | 35.0% | |
| ATTGCATCAAGAAGGGAAAT+GGG | + | chr7.3:57419518-57419537 | MS.gene38338:CDS | 35.0% | |
| CCATGTTCGATTTCAGAATT+AGG | - | chr7.3:57419562-57419581 | None:intergenic | 35.0% | |
| CTTCTTGATGCAATCAATGT+GGG | - | chr7.3:57419512-57419531 | None:intergenic | 35.0% | |
| TCAGTTCATACAACAGTATG+TGG | + | chr7.3:57419385-57419404 | MS.gene38338:CDS | 35.0% | |
| TCTTACCCCATTAATGGAAA+TGG | + | chr7.3:57419667-57419686 | MS.gene38338:CDS | 35.0% | |
| ! | CACATTGATTGCATCAAGAA+GGG | + | chr7.3:57419511-57419530 | MS.gene38338:CDS | 35.0% |
| !! | CCTAATTCTGAAATCGAACA+TGG | + | chr7.3:57419559-57419578 | MS.gene38338:CDS | 35.0% |
| ATCGGGCTTATGACTGTAAA+TGG | - | chr7.3:57419328-57419347 | None:intergenic | 40.0% | |
| ATGCTTCTACCTTCTTCATC+GGG | - | chr7.3:57419345-57419364 | None:intergenic | 40.0% | |
| CATGCTTCTACCTTCTTCAT+CGG | - | chr7.3:57419346-57419365 | None:intergenic | 40.0% | |
| CCTTCTTGATGCAATCAATG+TGG | - | chr7.3:57419513-57419532 | None:intergenic | 40.0% | |
| GATTGCATCAAGAAGGGAAA+TGG | + | chr7.3:57419517-57419536 | MS.gene38338:CDS | 40.0% | |
| TCGGGCTTATGACTGTAAAT+GGG | - | chr7.3:57419327-57419346 | None:intergenic | 40.0% | |
| ! | CCACATTGATTGCATCAAGA+AGG | + | chr7.3:57419510-57419529 | MS.gene38338:CDS | 40.0% |
| ! | GGCTTATGTTAAGCTTGCTT+TGG | + | chr7.3:57418960-57418979 | MS.gene38338:CDS | 40.0% |
| ! | TAGAAGCATGTGACACAACA+TGG | + | chr7.3:57419356-57419375 | MS.gene38338:CDS | 40.0% |
| !! | ATTCTGAAATCGAACATGGC+TGG | + | chr7.3:57419563-57419582 | MS.gene38338:CDS | 40.0% |
| ACGACCATCTCTTACGAAGA+TGG | + | chr7.3:57419459-57419478 | MS.gene38338:CDS | 45.0% | |
| AGTCATAAGCCCGATGAAGA+AGG | + | chr7.3:57419333-57419352 | MS.gene38338:intron | 45.0% | |
| ATCTTCGTAAGAGATGGTCG+TGG | - | chr7.3:57419460-57419479 | None:intergenic | 45.0% | |
| CATACAACAGTATGTGGCTC+CGG | + | chr7.3:57419391-57419410 | MS.gene38338:CDS | 45.0% | |
| GAGAATCTCGAAGTAGCGTT+CGG | - | chr7.3:57419611-57419630 | None:intergenic | 45.0% | |
| TCGACCATCTTCGTAAGAGA+TGG | - | chr7.3:57419466-57419485 | None:intergenic | 45.0% | |
| TGGGGTAAGATACAGTCTTG+AGG | - | chr7.3:57419657-57419676 | None:intergenic | 45.0% | |
| ACAGTATGTGGCTCCGGATG+TGG | + | chr7.3:57419397-57419416 | MS.gene38338:CDS | 55.0% | |
| CACCAATTATTCCCCACGCG+AGG | - | chr7.3:57419426-57419445 | None:intergenic | 55.0% | |
| CTCGCGTGGGGAATAATTGG+TGG | + | chr7.3:57419424-57419443 | MS.gene38338:CDS | 55.0% | |
| GCGTTCGGCTTCAACGTTAG+AGG | - | chr7.3:57419596-57419615 | None:intergenic | 55.0% | |
| TGCCTCGCGTGGGGAATAAT+TGG | + | chr7.3:57419421-57419440 | MS.gene38338:CDS | 55.0% | |
| CCACGCGAGGCAACCACATC+CGG | - | chr7.3:57419413-57419432 | None:intergenic | 65.0% | |
| CGGATGTGGTTGCCTCGCGT+GGG | + | chr7.3:57419411-57419430 | MS.gene38338:CDS | 65.0% | |
| GGATGTGGTTGCCTCGCGTG+GGG | + | chr7.3:57419412-57419431 | MS.gene38338:CDS | 65.0% | |
| CCGGATGTGGTTGCCTCGCG+TGG | + | chr7.3:57419410-57419429 | MS.gene38338:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 57418958 | 57419723 | 57418958 | ID=MS.gene38338 |
| chr7.3 | mRNA | 57418958 | 57419723 | 57418958 | ID=MS.gene38338.t1;Parent=MS.gene38338 |
| chr7.3 | exon | 57418958 | 57419006 | 57418958 | ID=MS.gene38338.t1.exon1;Parent=MS.gene38338.t1 |
| chr7.3 | CDS | 57418958 | 57419006 | 57418958 | ID=cds.MS.gene38338.t1;Parent=MS.gene38338.t1 |
| chr7.3 | exon | 57419335 | 57419723 | 57419335 | ID=MS.gene38338.t1.exon2;Parent=MS.gene38338.t1 |
| chr7.3 | CDS | 57419335 | 57419723 | 57419335 | ID=cds.MS.gene38338.t1;Parent=MS.gene38338.t1 |
| Gene Sequence |
| Protein sequence |
