AlfalfaGEDB | MS.gene054805

M. sativa cultivar XinJiangDaYe / MS.gene054805

KEGG: -
GO: GO:0003674 GO:0003824 GO:0009055 GO:0016491
PFAM: PF02298.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054805.t1	XP_003602846.1	97	100	3	0	1	100	17	116	6.10E-50	206.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054805.t1	Q41001	87.5	104	12	1	1	103	18	121	1.4e-47	189.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054805.t1	B7FHG1	97.0	100	3	0	1	100	17	116	4.4e-50	206.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054805	MS.gene20375	0.810846	9.41E-51	-1.69E-46
MS.gene054805	MS.gene22697	0.806423	8.29E-50	-1.69E-46
MS.gene054805	MS.gene22779	0.817735	2.83E-52	-1.69E-46
MS.gene054805	MS.gene31203	0.803445	3.47E-49	-1.69E-46
MS.gene054805	MS.gene31204	0.804349	2.25E-49	-1.69E-46
MS.gene054805	MS.gene38580	0.839069	1.98E-57	-1.69E-46
MS.gene054805	MS.gene38601	0.813527	2.45E-51	-1.69E-46
MS.gene054805	MS.gene56020	0.801564	8.48E-49	-1.69E-46
MS.gene054805	MS.gene67694	0.803908	2.78E-49	-1.69E-46
MS.gene054805	MS.gene93852	0.827952	1.18E-54	-1.69E-46
MS.gene054805	MS.gene94683	0.819537	1.10E-52	-1.69E-46
MS.gene054805	MS.gene95624	0.826357	2.84E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene47150	MS.gene054805	PPI
MS.gene054805	MS.gene55509	PPI
MS.gene074086	MS.gene054805	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054805.t1	MTR_3g099580	97.087	103	3	0	1	103	17	119	8.25e-71	209
MS.gene054805.t1	MTR_3g099570	86.408	103	14	0	1	103	17	119	2.49e-61	185
MS.gene054805.t1	MTR_3g099680	81.905	105	17	2	1	103	17	121	2.13e-54	169
MS.gene054805.t1	MTR_3g099980	81.905	105	17	2	1	103	17	121	1.60e-53	166
MS.gene054805.t1	MTR_3g099540	69.903	103	31	0	1	103	18	120	3.91e-47	147
MS.gene054805.t1	MTR_8g007035	58.491	106	41	2	1	103	14	119	1.21e-36	123
MS.gene054805.t1	MTR_8g007020	57.547	106	42	2	1	103	14	119	1.56e-35	120
MS.gene054805.t1	MTR_1g090190	44.792	96	53	0	7	102	20	115	7.06e-28	100
MS.gene054805.t1	MTR_6g080660	47.872	94	49	0	10	103	28	121	7.36e-26	95.9
MS.gene054805.t1	MTR_4g114870	49.495	99	45	2	10	103	191	289	8.60e-26	98.6
MS.gene054805.t1	MTR_4g114870	47.525	101	48	2	8	103	27	127	1.49e-25	98.2
MS.gene054805.t1	MTR_2g088990	44.898	98	53	1	6	102	21	118	1.11e-25	95.9
MS.gene054805.t1	MTR_6g023760	44.898	98	53	1	6	102	22	119	3.84e-24	90.5
MS.gene054805.t1	MTR_5g006040	44.444	99	52	3	7	102	23	121	4.89e-24	90.5
MS.gene054805.t1	MTR_0334s0010	42.157	102	56	1	5	103	23	124	2.68e-23	89.0
MS.gene054805.t1	MTR_1g104800	42.424	99	54	3	7	102	22	120	1.08e-22	87.0
MS.gene054805.t1	MTR_1g112700	38.614	101	60	2	5	103	28	128	4.78e-22	85.5
MS.gene054805.t1	MTR_7g086140	43.434	99	54	2	6	102	22	120	2.14e-21	83.2
MS.gene054805.t1	MTR_7g086190	43.434	99	54	2	6	102	22	120	2.14e-21	83.2
MS.gene054805.t1	MTR_8g099220	41.584	101	55	2	7	103	26	126	2.48e-20	79.3
MS.gene054805.t1	MTR_4g067200	38.182	110	61	3	1	103	19	128	8.22e-20	79.3
MS.gene054805.t1	MTR_4g066110	38.182	110	61	3	1	103	19	128	8.22e-20	79.3
MS.gene054805.t1	MTR_7g086160	41.837	98	55	2	7	102	24	121	1.07e-19	79.0
MS.gene054805.t1	MTR_6g083240	41.905	105	54	3	5	102	22	126	1.35e-19	79.0
MS.gene054805.t1	MTR_2g043730	43.000	100	53	3	5	103	27	123	2.43e-19	77.0
MS.gene054805.t1	MTR_1g105130	39.394	99	58	2	6	102	21	119	2.53e-19	77.8
MS.gene054805.t1	MTR_1g105120	39.394	99	58	2	6	102	21	119	2.53e-19	77.8
MS.gene054805.t1	MTR_4g081100	42.708	96	50	2	12	102	34	129	1.40e-18	77.4
MS.gene054805.t1	MTR_2g025580	39.583	96	56	2	10	103	27	122	3.97e-18	75.1
MS.gene054805.t1	MTR_3g089035	43.299	97	51	3	7	102	30	123	5.90e-18	73.2
MS.gene054805.t1	MTR_7g086220	37.374	99	60	2	6	102	21	119	7.17e-18	73.9
MS.gene054805.t1	MTR_7g086100	39.785	93	55	1	11	102	27	119	1.44e-17	74.7
MS.gene054805.t1	MTR_7g086090	40.860	93	54	1	11	102	27	119	1.90e-17	74.7
MS.gene054805.t1	MTR_2g083250	41.837	98	50	2	12	102	28	125	2.55e-17	74.7
MS.gene054805.t1	MTR_7g086280	37.755	98	60	1	6	102	22	119	5.45e-17	72.0
MS.gene054805.t1	MTR_7g086230	37.755	98	60	1	6	102	22	119	5.81e-17	72.0
MS.gene054805.t1	MTR_4g007250	39.000	100	59	2	4	102	23	121	1.53e-16	70.9
MS.gene054805.t1	MTR_1g090420	34.653	101	62	2	7	103	25	125	4.21e-16	70.9
MS.gene054805.t1	MTR_6g022170	36.275	102	59	2	7	103	31	131	2.55e-15	67.4
MS.gene054805.t1	MTR_8g463180	38.298	94	55	2	12	102	26	119	2.73e-15	69.7
MS.gene054805.t1	MTR_4g111640	33.333	102	61	2	8	103	30	130	8.96e-15	66.6
MS.gene054805.t1	MTR_2g101300	36.275	102	59	2	7	103	24	124	3.31e-14	65.1
MS.gene054805.t1	MTR_6g013420	38.462	104	59	4	3	102	30	132	5.31e-14	64.3
MS.gene054805.t1	MTR_4g077787	32.353	102	62	2	8	103	30	130	7.13e-14	64.3
MS.gene054805.t1	MTR_8g088830	39.000	100	52	5	7	102	25	119	1.29e-13	62.0
MS.gene054805.t1	MTR_6g013200	37.234	94	55	3	12	102	37	129	2.78e-13	62.4
MS.gene054805.t1	MTR_4g112310	35.000	80	51	1	24	103	2	80	4.70e-13	60.8
MS.gene054805.t1	MTR_2g090575	40.217	92	52	2	12	100	27	118	6.06e-13	63.2
MS.gene054805.t1	MTR_1g014120	35.789	95	56	2	13	102	29	123	9.89e-13	61.2
MS.gene054805.t1	MTR_3g105930	34.737	95	57	2	13	102	31	125	1.88e-12	60.5
MS.gene054805.t1	MTR_2g090580	37.234	94	56	2	12	102	29	122	2.04e-12	60.5
MS.gene054805.t1	MTR_4g124280	33.663	101	62	2	7	102	22	122	3.29e-12	60.5
MS.gene054805.t1	MTR_4g130800	40.260	77	45	1	27	102	50	126	3.45e-12	60.8
MS.gene054805.t1	MTR_4g130800	40.260	77	45	1	27	102	51	127	3.84e-12	60.8
MS.gene054805.t1	MTR_4g130780	37.500	80	49	1	24	102	48	127	9.21e-12	58.5
MS.gene054805.t1	MTR_8g086360	38.542	96	52	3	12	102	26	119	5.68e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054805.t1	AT1G72230	72.941	85	23	0	18	102	31	115	3.79e-43	139
MS.gene054805.t1	AT1G22480	65.882	85	29	0	18	102	27	111	4.36e-35	118
MS.gene054805.t1	AT2G32300	48.000	100	51	1	5	103	21	120	3.13e-29	105
MS.gene054805.t1	AT2G32300	48.000	100	51	1	5	103	51	150	8.88e-29	105
MS.gene054805.t1	AT3G60270	46.465	99	53	0	5	103	21	119	1.10e-27	100
MS.gene054805.t1	AT3G27200	44.898	98	53	1	6	102	22	119	3.96e-26	95.5
MS.gene054805.t1	AT5G07475	44.330	97	54	0	7	103	27	123	4.22e-25	93.6
MS.gene054805.t1	AT2G26720	45.361	97	51	2	8	102	27	123	1.74e-24	92.4
MS.gene054805.t1	AT2G31050	47.423	97	49	2	8	102	27	123	3.84e-24	91.3
MS.gene054805.t1	AT2G44790	51.136	88	41	1	18	103	36	123	1.62e-23	89.7
MS.gene054805.t1	AT5G26330	44.444	99	52	2	7	102	21	119	3.60e-23	88.6
MS.gene054805.t1	AT3G17675	37.500	104	64	1	1	103	23	126	1.19e-20	80.5
MS.gene054805.t1	AT3G60280	42.268	97	56	0	6	102	20	116	2.60e-20	81.6
MS.gene054805.t1	AT5G20230	41.237	97	53	2	10	102	25	121	4.80e-16	70.1
MS.gene054805.t1	AT2G25060	40.816	98	52	3	11	102	32	129	7.14e-16	69.3
MS.gene054805.t1	AT1G64640	38.000	100	58	2	7	102	30	129	1.54e-15	68.6
MS.gene054805.t1	AT3G18590	36.893	103	58	2	7	103	24	125	4.13e-15	67.4
MS.gene054805.t1	AT5G57920	39.241	79	47	1	25	102	43	121	1.19e-14	66.2
MS.gene054805.t1	AT5G57920	39.241	79	47	1	25	102	42	120	1.97e-14	65.5
MS.gene054805.t1	AT5G57920	39.241	79	47	1	25	102	75	153	2.52e-14	65.9
MS.gene054805.t1	AT4G31840	38.776	98	54	3	11	102	28	125	2.67e-14	65.1
MS.gene054805.t1	AT1G45063	39.000	100	53	4	9	102	148	245	2.92e-14	66.6
MS.gene054805.t1	AT1G45063	33.663	101	60	3	7	102	26	124	9.32e-11	57.0
MS.gene054805.t1	AT1G45063	39.000	100	53	4	9	102	148	245	4.06e-14	66.6
MS.gene054805.t1	AT4G12880	36.364	99	59	3	7	102	26	123	1.32e-13	62.8
MS.gene054805.t1	AT5G53870	36.170	94	57	2	12	102	32	125	2.37e-13	64.7
MS.gene054805.t1	AT4G30590	36.709	79	49	1	25	102	48	126	2.47e-13	62.8
MS.gene054805.t1	AT2G02850	39.000	100	51	4	7	102	33	126	3.45e-13	61.2
MS.gene054805.t1	AT4G28365	35.789	95	58	2	12	103	31	125	4.72e-13	62.4
MS.gene054805.t1	AT5G15350	36.364	99	61	2	7	103	26	124	5.35e-13	61.6
MS.gene054805.t1	AT1G48940	37.113	97	54	3	13	103	29	124	1.05e-12	60.8
MS.gene054805.t1	AT4G32490	32.979	94	60	2	12	102	33	126	5.67e-12	59.7
MS.gene054805.t1	AT1G79800	35.052	97	57	2	12	103	36	131	1.18e-11	58.5
MS.gene054805.t1	AT3G53330	33.654	104	64	4	3	102	182	284	1.27e-11	59.7
MS.gene054805.t1	AT5G53870	36.170	94	57	2	12	102	32	125	1.35e-11	59.7
MS.gene054805.t1	AT4G01380	32.979	94	53	4	17	102	79	170	4.09e-11	57.4

Find 0 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATATTAGATTTGTAAATTG+TGG	-	21142:21272-21291	MS.gene054805:intergenic	15.0%
!!	TGGTTCTGATTATAACACAT+GGG	+	21142:21082-21101	MS.gene054805:CDS	30.0%
!!	TTGGTTCTGATTATAACACA+TGG	+	21142:21081-21100	MS.gene054805:CDS	30.0%
	ACTATGACAGTGTTCAACTA+TGG	+	21142:21301-21320	MS.gene054805:intron	35.0%
	GACTATAAATCATGCACAAC+TGG	+	21142:21358-21377	MS.gene054805:CDS	35.0%
	TCAATTAGTACAGACAGTAG+TGG	+	21142:21385-21404	MS.gene054805:CDS	35.0%
	AGTGACAAAACATTTGCGGT+TGG	+	21142:21107-21126	MS.gene054805:CDS	40.0%
	CAGCTTAATGAGAAGAAAGC+AGG	+	21142:21173-21192	MS.gene054805:intron	40.0%
	CCAACCTGATTTATCTCCAA+CGG	-	21142:21057-21076	MS.gene054805:intergenic	40.0%
	CGTTGGAGATAAATCAGGTT+GGG	+	21142:21055-21074	MS.gene054805:CDS	40.0%
	GGCTAGTGACAAAACATTTG+CGG	+	21142:21103-21122	MS.gene054805:CDS	40.0%
!	TACTTCATATGTGCTGTTCC+TGG	+	21142:21445-21464	MS.gene054805:CDS	40.0%
!!	GATAAATCAGGTTGGGCAAT+TGG	+	21142:21062-21081	MS.gene054805:CDS	40.0%
	ACAGTGTTCAACTATGGTGC+TGG	+	21142:21307-21326	MS.gene054805:intron	45.0%
	CACACCGTTGGAGATAAATC+AGG	+	21142:21050-21069	MS.gene054805:CDS	45.0%
	CCGTTGGAGATAAATCAGGT+TGG	+	21142:21054-21073	MS.gene054805:CDS	45.0%
	CTGATTTATCTCCAACGGTG+TGG	-	21142:21052-21071	MS.gene054805:intergenic	45.0%
	GTGTTCCTGCTTTCTTGAGA+GGG	-	21142:21423-21442	MS.gene054805:intergenic	45.0%
	TGTGTTCCTGCTTTCTTGAG+AGG	-	21142:21424-21443	MS.gene054805:intergenic	45.0%
	TTTCTTGAGAGGGATGGTTG+TGG	-	21142:21413-21432	MS.gene054805:intergenic	45.0%
	ACCATCCCTCTCAAGAAAGC+AGG	+	21142:21415-21434	MS.gene054805:CDS	50.0%
	GTGTGGACAGTTGCAAGAGT+TGG	-	21142:21035-21054	MS.gene054805:intergenic	50.0%
	TCCTGCTTTCTTGAGAGGGA+TGG	-	21142:21419-21438	MS.gene054805:intergenic	50.0%
!!	TTTGCGGTTGGTGACAGTCT+TGG	+	21142:21119-21138	MS.gene054805:CDS	50.0%
	ATGCCACCTGTGCAATGTCC+AGG	-	21142:21466-21485	MS.gene054805:intergenic	55.0%
	CTTGCAACTGTCCACACCGT+TGG	+	21142:21038-21057	MS.gene054805:CDS	55.0%
	GCTGTTCCTGGACATTGCAC+AGG	+	21142:21457-21476	MS.gene054805:CDS	55.0%
	GTTCCTGGACATTGCACAGG+TGG	+	21142:21460-21479	MS.gene054805:CDS	55.0%
!	CTATGGTGCTGGACACACAG+TGG	+	21142:21318-21337	MS.gene054805:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
21142	gene	21023	21501	21023	ID=MS.gene054805
21142	mRNA	21023	21501	21023	ID=MS.gene054805.t1;Parent=MS.gene054805
21142	exon	21023	21140	21023	ID=MS.gene054805.t1.exon1;Parent=MS.gene054805.t1
21142	CDS	21023	21140	21023	ID=cds.MS.gene054805.t1;Parent=MS.gene054805.t1
21142	exon	21311	21501	21311	ID=MS.gene054805.t1.exon2;Parent=MS.gene054805.t1
21142	CDS	21311	21501	21311	ID=cds.MS.gene054805.t1;Parent=MS.gene054805.t1

Gene Sequence

>MS.gene054805

ATGGCACTGCCAACTCTTGCAACTGTCCACACCGTTGGAGATAAATCAGGTTGGGCAATTGGTTCTGATTATAACACATGGGCTAGTGACAAAACATTTGCGGTTGGTGACAGTCTTGGTAAGTATTATTAATTATTTCTTTGTTAAACACAGCTTAATGAGAAGAAAGCAGGTAAAGATTGTAAACTGAAAAATAGTTAATTGAAGTTTTGTCAATTTCATAATTCATTTAGTTTTATATTGCAACCACAATTTACAAATCTAATATATGTAACTATACTATGACAGTGTTCAACTATGGTGCTGGACACACAGTGGATGAAGTTAAAGAAAGTGACTATAAATCATGCACAACTGGAAATTCAATTAGTACAGACAGTAGTGGTGCCACAACCATCCCTCTCAAGAAAGCAGGAACACATTACTTCATATGTGCTGTTCCTGGACATTGCACAGGTGGCATGAAACTTTCTGTTAAG

Protein sequence

>MS.gene054805.t1

MALPTLATVHTVGDKSGWAIGSDYNTWASDKTFAVGDSLVFNYGAGHTVDEVKESDYKSCTTGNSISTDSSGATTIPLKKAGTHYFICAVPGHCTGGMKLSVK