AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38925


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38925.t1 MTR_2g069350 43.750 384 146 11 1 333 1 365 1.47e-88 270
MS.gene38925.t1 MTR_2g028830 33.889 360 169 9 1 333 1 318 5.20e-58 190
MS.gene38925.t1 MTR_4g083540 35.465 344 159 11 1 333 1 292 7.11e-58 189
MS.gene38925.t1 MTR_5g013690 31.556 225 129 3 109 333 135 334 1.36e-30 118
MS.gene38925.t1 MTR_5g013690 31.556 225 129 3 109 333 72 271 2.77e-30 116
MS.gene38925.t1 MTR_1g019250 28.251 223 134 5 109 331 142 338 3.51e-23 98.6
MS.gene38925.t1 MTR_4g099060 30.222 225 129 3 109 333 134 330 3.44e-22 95.5
MS.gene38925.t1 MTR_4g099060 30.222 225 129 3 109 333 89 285 4.35e-22 94.7
MS.gene38925.t1 MTR_4g036485 26.457 223 118 5 101 321 21 199 2.04e-20 88.2
MS.gene38925.t1 MTR_3g117970 27.706 231 112 6 104 329 11 191 7.03e-18 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38925.t1 AT3G12920 38.719 359 168 13 1 333 1 333 1.13e-64 208
MS.gene38925.t1 AT5G45100 35.588 340 164 10 1 333 1 292 5.75e-59 192
MS.gene38925.t1 AT1G79110 35.484 372 182 11 1 333 1 353 2.58e-57 189
MS.gene38925.t1 AT1G79110 35.200 375 182 12 1 333 1 356 3.55e-55 184
MS.gene38925.t1 AT1G79110 43.231 229 117 4 107 333 114 331 3.59e-55 183
MS.gene38925.t1 AT5G45100 37.643 263 122 6 73 333 43 265 6.29e-55 181
MS.gene38925.t1 AT4G19700 33.819 343 176 11 1 333 1 302 8.05e-53 176
MS.gene38925.t1 AT4G35070 31.963 219 118 6 114 331 19 207 1.40e-25 102
MS.gene38925.t1 AT4G35070 32.258 217 116 6 114 329 74 260 2.09e-25 103
MS.gene38925.t1 AT1G32740 29.279 222 129 6 110 329 112 307 7.72e-24 100
MS.gene38925.t1 AT1G32740 29.279 222 129 6 110 329 101 296 7.82e-24 99.8
MS.gene38925.t1 AT1G32740 29.279 222 129 6 110 329 68 263 8.15e-24 99.0
MS.gene38925.t1 AT1G32740 29.279 222 129 6 110 329 68 263 8.15e-24 99.0
MS.gene38925.t1 AT1G60610 28.139 231 145 5 101 331 128 337 1.85e-23 99.4
MS.gene38925.t1 AT1G60610 28.139 231 145 5 101 331 128 337 1.85e-23 99.4
MS.gene38925.t1 AT1G60610 28.139 231 145 5 101 331 128 337 1.85e-23 99.4
MS.gene38925.t1 AT1G60610 28.139 231 145 5 101 331 128 337 1.85e-23 99.4
MS.gene38925.t1 AT1G60610 28.139 231 145 5 101 331 128 337 1.85e-23 99.4
MS.gene38925.t1 AT1G45976 28.700 223 126 5 109 331 134 323 2.25e-23 99.0
MS.gene38925.t1 AT1G10650 29.302 215 127 4 118 331 90 280 2.69e-21 92.4
MS.gene38925.t1 AT1G10650 28.037 214 131 3 118 331 146 336 2.94e-21 93.2
MS.gene38925.t1 AT4G17680 30.000 220 125 5 113 331 121 312 5.83e-21 92.0
MS.gene38925.t1 AT4G17680 30.000 220 125 5 113 331 49 240 7.72e-21 90.1
MS.gene38925.t1 AT5G47050 27.126 247 150 4 89 331 77 297 5.90e-17 80.5
MS.gene38925.t1 AT4G17680 31.100 209 112 7 125 331 10 188 2.90e-16 76.3

Find 51 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCACTGTTACCACTTTC+AGG 0.214320 8.4:-50405905 MS.gene38925:CDS
GTTGAAGTTGAGAAGGAAAA+AGG 0.285209 8.4:+50406112 None:intergenic
AGGGTAGTTATTGTTATTGA+TGG 0.337872 8.4:+50405724 None:intergenic
GAGAGAAACAATGATGATAA+TGG 0.367299 8.4:-50405496 MS.gene38925:CDS
CTTCTCAACTTCAACCCAAT+AGG 0.388096 8.4:-50406104 MS.gene38925:intron
TGCCGTTGCTGCGGCGGTGG+AGG 0.390131 8.4:-50405217 MS.gene38925:CDS
GTGTTCAATGTGTGGGGCTA+TGG 0.402214 8.4:-50405010 MS.gene38925:CDS
AGTAATGACGGCGCAGGATA+AGG 0.404721 8.4:-50405139 MS.gene38925:CDS
AAGATTGTACTATACCTATT+GGG 0.415314 8.4:+50406090 None:intergenic
ACAAATCCTTATGCAACAAA+TGG 0.418067 8.4:-50405292 MS.gene38925:CDS
ATATTCATCATTACTACAAC+AGG 0.424115 8.4:+50405179 None:intergenic
CGTTGCTGCGGCGGTGGAGG+CGG 0.430174 8.4:-50405214 MS.gene38925:CDS
TGCTGCGGCGGTGGAGGCGG+AGG 0.433823 8.4:-50405211 MS.gene38925:CDS
TATGCATGGAGAATCAAATC+TGG 0.461576 8.4:-50405371 MS.gene38925:CDS
TATGTTTGTGTTCAATGTGT+GGG 0.464104 8.4:-50405017 MS.gene38925:CDS
GAAGATGGTGTCTGGTTTCA+CGG 0.465973 8.4:+50405793 None:intergenic
CGCCTCCACCGCCGCAGCAA+CGG 0.480109 8.4:+50405215 None:intergenic
TTGTAAGCGAAGATGGTGTC+TGG 0.480918 8.4:+50405785 None:intergenic
AACGTTGTTGTAAGCGAAGA+TGG 0.493808 8.4:+50405778 None:intergenic
CACAAACATAAGTGTCTACA+AGG 0.505076 8.4:+50405030 None:intergenic
CGGAGGAAATCGGTTATGAA+AGG 0.505897 8.4:+50405830 None:intergenic
GATTTAAAAGAATCACATAT+AGG 0.512400 8.4:+50404976 None:intergenic
AAGGATGAAGGTACTAGTAG+TGG 0.520250 8.4:-50405120 MS.gene38925:CDS
AAAAGTGAAAACACTATGCA+TGG 0.524997 8.4:-50405385 MS.gene38925:CDS
AACAACAACAACGGCTGCGA+CGG 0.528600 8.4:-50405261 MS.gene38925:CDS
TGTAGTAATGATGAATATTG+TGG 0.529768 8.4:-50405174 MS.gene38925:CDS
ATAGATTATGCAGAAACTGT+GGG 0.549558 8.4:-50405080 MS.gene38925:CDS
TTATGTTTGTGTTCAATGTG+TGG 0.554213 8.4:-50405018 MS.gene38925:CDS
GTTGAGATGGTGAGCATCGA+CGG 0.556642 8.4:+50406134 None:intergenic
TTAGACGGAGAAGTTTAGAG+TGG 0.557294 8.4:-50405538 MS.gene38925:intron
CATGTTTGTATAATTTGTGT+CGG 0.569763 8.4:+50405940 None:intergenic
GTAGTAATGATGAATATTGT+GGG 0.574573 8.4:-50405173 MS.gene38925:CDS
CTATTGGGTTGAAGTTGAGA+AGG 0.576004 8.4:+50406105 None:intergenic
GGAGGAAATCGGTTATGAAA+GGG 0.577225 8.4:+50405831 None:intergenic
AATAGATTATGCAGAAACTG+TGG 0.580193 8.4:-50405081 MS.gene38925:CDS
TTATGCAGAAACTGTGGGGA+AGG 0.589265 8.4:-50405075 MS.gene38925:CDS
ATGTTTGTGTTCAATGTGTG+GGG 0.595264 8.4:-50405016 MS.gene38925:CDS
ACGGCGCAGGATAAGGATGA+AGG 0.596413 8.4:-50405132 MS.gene38925:CDS
TGAATATTGTGGGTGGCGTA+CGG 0.612792 8.4:-50405163 MS.gene38925:CDS
GTTATGAAAGGGTTGTTCGA+CGG 0.613447 8.4:+50405842 None:intergenic
ACGGTGGTTGTGCCTGAAAG+TGG 0.615359 8.4:+50405893 None:intergenic
TTGTTCGACGGAGATTGCGA+CGG 0.619398 8.4:+50405854 None:intergenic
TACGGTAGTAATGACGGCGC+AGG 0.633033 8.4:-50405145 MS.gene38925:CDS
TTCAGGCACAACCACCGTGT+CGG 0.634510 8.4:-50405888 MS.gene38925:CDS
AGAAATGAAGTTGTCGATGT+CGG 0.644465 8.4:+50405658 None:intergenic
GTGGCGTACGGTAGTAATGA+CGG 0.665628 8.4:-50405151 MS.gene38925:CDS
TGCGACGGTGAATATAGCGG+TGG 0.671652 8.4:-50405246 MS.gene38925:CDS
GGCTGCGACGGTGAATATAG+CGG 0.693061 8.4:-50405249 MS.gene38925:CDS
TAGATTATGCAGAAACTGTG+GGG 0.699966 8.4:-50405079 MS.gene38925:CDS
GTAATGATGAATATTGTGGG+TGG 0.732674 8.4:-50405170 MS.gene38925:CDS
GATGGTGTCTGGTTTCACGG+TGG 0.784629 8.4:+50405796 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAACAAAACAAAAAACA+TGG + chr8.4:50405058-50405077 None:intergenic 20.0%
!! GAAAAAAATTTAGTAGAGTA+AGG + chr8.4:50405472-50405491 None:intergenic 20.0%
!! GATTTAAAAGAATCACATAT+AGG + chr8.4:50406113-50406132 None:intergenic 20.0%
!!! TGTTCATTCTTTCAATTTTT+TGG + chr8.4:50405668-50405687 None:intergenic 20.0%
! AAAAATCTTCACTGATGAAA+GGG + chr8.4:50405384-50405403 None:intergenic 25.0%
! AAATTTAGTAGAGTAAGGTA+AGG + chr8.4:50405467-50405486 None:intergenic 25.0%
! AAGATTGTACTATACCTATT+GGG + chr8.4:50404999-50405018 None:intergenic 25.0%
! ATATTCATCATTACTACAAC+AGG + chr8.4:50405910-50405929 None:intergenic 25.0%
! CAAAACAAAAAACATGGTTT+AGG + chr8.4:50405052-50405071 None:intergenic 25.0%
! GTAGTAATGATGAATATTGT+GGG - chr8.4:50405913-50405932 MS.gene38925:CDS 25.0%
! TGTAGTAATGATGAATATTG+TGG - chr8.4:50405912-50405931 MS.gene38925:CDS 25.0%
!! CATGTTTGTATAATTTGTGT+CGG + chr8.4:50405149-50405168 None:intergenic 25.0%
!!! TTGCATGTGTTTTTTTTAGA+CGG - chr8.4:50405533-50405552 MS.gene38925:CDS 25.0%
AAAAGTGAAAACACTATGCA+TGG - chr8.4:50405701-50405720 MS.gene38925:CDS 30.0%
AATAGATTATGCAGAAACTG+TGG - chr8.4:50406005-50406024 MS.gene38925:intron 30.0%
ACAAATCCTTATGCAACAAA+TGG - chr8.4:50405794-50405813 MS.gene38925:CDS 30.0%
ATAGATTATGCAGAAACTGT+GGG - chr8.4:50406006-50406025 MS.gene38925:intron 30.0%
GAAAAATCTTCACTGATGAA+AGG + chr8.4:50405385-50405404 None:intergenic 30.0%
GAAAACAACAACAACAACAA+CGG - chr8.4:50405816-50405835 MS.gene38925:CDS 30.0%
GAAGATTGTACTATACCTAT+TGG + chr8.4:50405000-50405019 None:intergenic 30.0%
GAGAGAAACAATGATGATAA+TGG - chr8.4:50405590-50405609 MS.gene38925:intron 30.0%
TATGTTTGTGTTCAATGTGT+GGG - chr8.4:50406069-50406088 MS.gene38925:intron 30.0%
TTATGTTTGTGTTCAATGTG+TGG - chr8.4:50406068-50406087 MS.gene38925:intron 30.0%
! AGGGTAGTTATTGTTATTGA+TGG + chr8.4:50405365-50405384 None:intergenic 30.0%
!! TGTTTTCCATTTGTTGCATA+AGG + chr8.4:50405803-50405822 None:intergenic 30.0%
!!! AATCTCTAGAACGTTTTTGT+GGG + chr8.4:50405342-50405361 None:intergenic 30.0%
AGAAATGAAGTTGTCGATGT+CGG + chr8.4:50405431-50405450 None:intergenic 35.0%
ATGTTTGTGTTCAATGTGTG+GGG - chr8.4:50406070-50406089 MS.gene38925:intron 35.0%
CACAAACATAAGTGTCTACA+AGG + chr8.4:50406059-50406078 None:intergenic 35.0%
GTAATGATGAATATTGTGGG+TGG - chr8.4:50405916-50405935 MS.gene38925:CDS 35.0%
GTTGAAGTTGAGAAGGAAAA+AGG + chr8.4:50404977-50404996 None:intergenic 35.0%
TAGATTATGCAGAAACTGTG+GGG - chr8.4:50406007-50406026 MS.gene38925:intron 35.0%
TATGCATGGAGAATCAAATC+TGG - chr8.4:50405715-50405734 MS.gene38925:CDS 35.0%
!!! GAATCTCTAGAACGTTTTTG+TGG + chr8.4:50405343-50405362 None:intergenic 35.0%
AACGTTGTTGTAAGCGAAGA+TGG + chr8.4:50405311-50405330 None:intergenic 40.0%
CTTCTCAACTTCAACCCAAT+AGG - chr8.4:50404982-50405001 MS.gene38925:CDS 40.0%
GAGAAGGAAAAAGGTTGAGA+TGG + chr8.4:50404968-50404987 None:intergenic 40.0%
GGAGGAAATCGGTTATGAAA+GGG + chr8.4:50405258-50405277 None:intergenic 40.0%
GTTATGAAAGGGTTGTTCGA+CGG + chr8.4:50405247-50405266 None:intergenic 40.0%
TCATCACTGTTACCACTTTC+AGG - chr8.4:50405181-50405200 MS.gene38925:CDS 40.0%
TTAGACGGAGAAGTTTAGAG+TGG - chr8.4:50405548-50405567 MS.gene38925:intron 40.0%
! TGTCTTTTGACGGAGGAAAT+CGG + chr8.4:50405269-50405288 None:intergenic 40.0%
!! AAGGATGAAGGTACTAGTAG+TGG - chr8.4:50405966-50405985 MS.gene38925:CDS 40.0%
!! CTATTGGGTTGAAGTTGAGA+AGG + chr8.4:50404984-50405003 None:intergenic 40.0%
CGGAGGAAATCGGTTATGAA+AGG + chr8.4:50405259-50405278 None:intergenic 45.0%
TGAATATTGTGGGTGGCGTA+CGG - chr8.4:50405923-50405942 MS.gene38925:CDS 45.0%
TTATGCAGAAACTGTGGGGA+AGG - chr8.4:50406011-50406030 MS.gene38925:intron 45.0%
TTGTAAGCGAAGATGGTGTC+TGG + chr8.4:50405304-50405323 None:intergenic 45.0%
! AATGAGATTTTCCGACACGG+TGG + chr8.4:50405212-50405231 None:intergenic 45.0%
! CGGAATGAGATTTTCCGACA+CGG + chr8.4:50405215-50405234 None:intergenic 45.0%
! GAAGATGGTGTCTGGTTTCA+CGG + chr8.4:50405296-50405315 None:intergenic 45.0%
! TCACGGTGGATGTCTTTTGA+CGG + chr8.4:50405279-50405298 None:intergenic 45.0%
!! TGTCGATGTCGGTGTTTTGA+CGG + chr8.4:50405420-50405439 None:intergenic 45.0%
AACAACAACAACGGCTGCGA+CGG - chr8.4:50405825-50405844 MS.gene38925:CDS 50.0%
AGTAATGACGGCGCAGGATA+AGG - chr8.4:50405947-50405966 MS.gene38925:CDS 50.0%
GTGGCGTACGGTAGTAATGA+CGG - chr8.4:50405935-50405954 MS.gene38925:CDS 50.0%
GTGTTCAATGTGTGGGGCTA+TGG - chr8.4:50406076-50406095 MS.gene38925:intron 50.0%
TTGTTCGACGGAGATTGCGA+CGG + chr8.4:50405235-50405254 None:intergenic 50.0%
! TGGTGATTTTGCCGTTGCTG+CGG - chr8.4:50405860-50405879 MS.gene38925:CDS 50.0%
!! GTTGAGATGGTGAGCATCGA+CGG + chr8.4:50404955-50404974 None:intergenic 50.0%
ACGGCGCAGGATAAGGATGA+AGG - chr8.4:50405954-50405973 MS.gene38925:CDS 55.0%
ACGGTGGTTGTGCCTGAAAG+TGG + chr8.4:50405196-50405215 None:intergenic 55.0%
GGCTGCGACGGTGAATATAG+CGG - chr8.4:50405837-50405856 MS.gene38925:CDS 55.0%
TACGGTAGTAATGACGGCGC+AGG - chr8.4:50405941-50405960 MS.gene38925:CDS 55.0%
TGCGACGGTGAATATAGCGG+TGG - chr8.4:50405840-50405859 MS.gene38925:CDS 55.0%
TTCAGGCACAACCACCGTGT+CGG - chr8.4:50405198-50405217 MS.gene38925:CDS 55.0%
! CGGTGGATGTCTTTTGACGG+AGG + chr8.4:50405276-50405295 None:intergenic 55.0%
! GATGGTGTCTGGTTTCACGG+TGG + chr8.4:50405293-50405312 None:intergenic 55.0%
! TGATTTTGCCGTTGCTGCGG+CGG - chr8.4:50405863-50405882 MS.gene38925:CDS 55.0%
!!! CGATGTCGGTGTTTTGACGG+TGG + chr8.4:50405417-50405436 None:intergenic 55.0%
! TTTTGCCGTTGCTGCGGCGG+TGG - chr8.4:50405866-50405885 MS.gene38925:CDS 65.0%
CGCCTCCACCGCCGCAGCAA+CGG + chr8.4:50405874-50405893 None:intergenic 75.0%
CGTTGCTGCGGCGGTGGAGG+CGG - chr8.4:50405872-50405891 MS.gene38925:CDS 75.0%
TGCCGTTGCTGCGGCGGTGG+AGG - chr8.4:50405869-50405888 MS.gene38925:CDS 75.0%
! TGCTGCGGCGGTGGAGGCGG+AGG - chr8.4:50405875-50405894 MS.gene38925:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 50404948 50406160 50404948 ID=MS.gene38925
chr8.4 mRNA 50404948 50406160 50404948 ID=MS.gene38925.t1;Parent=MS.gene38925
chr8.4 exon 50406105 50406160 50406105 ID=MS.gene38925.t1.exon1;Parent=MS.gene38925.t1
chr8.4 CDS 50406105 50406160 50406105 ID=cds.MS.gene38925.t1;Parent=MS.gene38925.t1
chr8.4 exon 50405652 50405991 50405652 ID=MS.gene38925.t1.exon2;Parent=MS.gene38925.t1
chr8.4 CDS 50405652 50405991 50405652 ID=cds.MS.gene38925.t1;Parent=MS.gene38925.t1
chr8.4 exon 50404948 50405556 50404948 ID=MS.gene38925.t1.exon3;Parent=MS.gene38925.t1
chr8.4 CDS 50404948 50405556 50404948 ID=cds.MS.gene38925.t1;Parent=MS.gene38925.t1
Gene Sequence

>MS.gene38925

ATGACCGTCGATGCTCACCATCTCAACCTTTTTCCTTCTCAACTTCAACCCAATAGGTATAGTACAATCTTCTTCACAAATTCAAAGTCATATTTTTTTAACCTAAACCATGTTTTTTGTTTTGTTTTGTTTTGTTTATGTTTTTAATTGATTTGTTATGATTTTGCAGAGACATGATGATGATGATGATGAACACGACCGACACAAATTATACAAACATGTACAACAACTACTCATCACTGTTACCACTTTCAGGCACAACCACCGTGTCGGAAAATCTCATTCCGTCGCAATCTCCGTCGAACAACCCTTTCATAACCGATTTCCTCCGTCAAAAGACATCCACCGTGAAACCAGACACCATCTTCGCTTACAACAACGTTGTCTCGTTCCCACAAAAACGTTCTAGAGATTCCATCAATAACAATAACTACCCTTTCATCAGTGAAGATTTTTCTCATTACATCCACCGTCAAAACACCGACATCGACAACTTCATTTCTCAACATGTAAATTCCTTACCTTACTCTACTAAATTTTTTTCTTTTTATTTATTTATTAAAAAAATAAAGCTGATTTTTTTGTTTGCATGTGTTTTTTTTAGACGGAGAAGTTTAGAGTGGAATTAGAAGAGAAAAGAAAGAGAGAAACAATGATGATAATGGAAGCAATAGAAAACGAAGTGATGAAGAAAATGAAAGCAAAAGAAGAAGAAATCCAAAAAATTGAAAGAATGAACATGATTCTTGAAGAAAAAGTGAAAACACTATGCATGGAGAATCAAATCTGGAGAGAGTTAGCACAAAACAATGAAGCAACAGCAAATGCTCTCAGAAACAATCTACAACAAATCCTTATGCAACAAATGGAAAACAACAACAACAACAACGGCTGCGACGGTGAATATAGCGGTGGTGATTTTGCCGTTGCTGCGGCGGTGGAGGCGGAGGATGCTGAGTCCTGTTGTAGTAATGATGAATATTGTGGGTGGCGTACGGTAGTAATGACGGCGCAGGATAAGGATGAAGGTACTAGTAGTGGTAGAGTGATGAATAACAATAGATTATGCAGAAACTGTGGGGAAGGTGAATCTTGTGTATTGATCATGCCTTGTAGACACTTATGTTTGTGTTCAATGTGTGGGGCTATGGTTCTTACTTGTCCTATATGTGATTCTTTTAAATCTGCTACTCTTAATGTTAATCTTGATTAA

Protein sequence

>MS.gene38925.t1

MTVDAHHLNLFPSQLQPNRDMMMMMMNTTDTNYTNMYNNYSSLLPLSGTTTVSENLIPSQSPSNNPFITDFLRQKTSTVKPDTIFAYNNVVSFPQKRSRDSINNNNYPFISEDFSHYIHRQNTDIDNFISQHTEKFRVELEEKRKRETMMIMEAIENEVMKKMKAKEEEIQKIERMNMILEEKVKTLCMENQIWRELAQNNEATANALRNNLQQILMQQMENNNNNNGCDGEYSGGDFAVAAAVEAEDAESCCSNDEYCGWRTVVMTAQDKDEGTSSGRVMNNNRLCRNCGEGESCVLIMPCRHLCLCSMCGAMVLTCPICDSFKSATLNVNLD