AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39163

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39163.t1 MTR_1g054525 96.875 192 6 0 1 192 1 192 3.00e-136 379
MS.gene39163.t1 MTR_4g013345 56.897 174 73 1 21 192 23 196 1.13e-67 205
MS.gene39163.t1 MTR_4g013350 57.303 178 71 2 20 192 20 197 2.80e-67 204
MS.gene39163.t1 MTR_4g013335 58.788 165 64 2 30 192 34 196 3.09e-67 204
MS.gene39163.t1 MTR_4g013320 57.576 165 68 1 30 192 35 199 1.42e-66 202
MS.gene39163.t1 MTR_4g013330 56.886 167 68 2 30 192 33 199 1.25e-64 197
MS.gene39163.t1 MTR_4g013325 62.759 145 52 1 50 192 55 199 2.38e-62 192
MS.gene39163.t1 MTR_7g093820 51.053 190 88 4 6 192 7 194 7.44e-60 185
MS.gene39163.t1 MTR_4g013310 50.610 164 81 0 29 192 34 197 8.64e-60 185
MS.gene39163.t1 MTR_7g093850 49.000 200 88 6 1 192 1 194 2.85e-59 184
MS.gene39163.t1 MTR_7g093830 49.744 195 93 4 1 192 1 193 9.46e-59 182
MS.gene39163.t1 MTR_7g070390 48.168 191 90 5 10 192 3 192 9.06e-58 180
MS.gene39163.t1 MTR_7g093790 46.667 195 97 4 1 192 1 191 1.52e-55 174
MS.gene39163.t1 MTR_4g013385 46.277 188 91 5 12 192 6 190 2.83e-55 174
MS.gene39163.t1 MTR_0433s0040 46.277 188 91 5 12 192 6 190 2.83e-55 174
MS.gene39163.t1 MTR_7g093870 51.765 170 74 4 30 192 25 193 1.40e-54 173
MS.gene39163.t1 MTR_4g013770 46.023 176 90 3 20 192 18 191 7.75e-50 160
MS.gene39163.t1 MTR_4g073950 50.000 152 73 2 44 192 41 192 6.90e-49 157
MS.gene39163.t1 MTR_4g013355 44.809 183 82 4 17 192 9 179 3.57e-47 152
MS.gene39163.t1 MTR_4g078885 48.993 149 72 3 46 192 39 185 6.91e-46 149
MS.gene39163.t1 MTR_3g034030 48.993 149 72 3 46 192 39 185 3.20e-45 148
MS.gene39163.t1 MTR_3g105630 43.850 187 92 5 14 192 8 189 4.38e-43 142
MS.gene39163.t1 MTR_1g056370 47.682 151 75 3 44 191 36 185 1.85e-42 141
MS.gene39163.t1 MTR_5g096120 43.850 187 95 4 12 192 7 189 7.51e-42 139
MS.gene39163.t1 MTR_3g105640 41.799 189 100 5 9 192 5 188 1.07e-38 131
MS.gene39163.t1 MTR_4g122110 36.301 146 91 1 49 192 35 180 2.98e-26 99.4
MS.gene39163.t1 MTR_4g122130 36.601 153 91 2 46 192 28 180 1.48e-25 97.4
MS.gene39163.t1 MTR_4g049570 33.824 136 90 0 43 178 27 162 1.71e-18 79.0
MS.gene39163.t1 MTR_4g049550 29.775 178 104 3 7 176 4 168 8.28e-17 74.7
MS.gene39163.t1 MTR_1g046490 38.889 90 54 1 81 170 121 209 4.12e-15 71.2
MS.gene39163.t1 MTR_1g046800 36.697 109 66 2 62 170 118 223 6.73e-15 70.9
MS.gene39163.t1 MTR_1g046500 40.000 90 53 1 81 170 135 223 1.52e-14 70.1
MS.gene39163.t1 MTR_8g099115 32.394 142 85 4 42 175 32 170 4.58e-14 67.8
MS.gene39163.t1 MTR_1g115510 32.353 170 98 6 37 191 186 353 1.29e-13 68.6
MS.gene39163.t1 MTR_7g021300 31.690 142 83 5 60 189 248 387 1.34e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39163.t1 AT1G55210 49.744 195 87 5 1 192 1 187 2.01e-60 187
MS.gene39163.t1 AT1G55210 49.744 195 87 5 1 192 1 187 2.01e-60 187
MS.gene39163.t1 AT1G58170 55.263 152 66 1 43 192 34 185 1.48e-59 184
MS.gene39163.t1 AT1G65870 53.488 172 75 3 22 191 21 189 3.94e-59 183
MS.gene39163.t1 AT5G49040 47.778 180 92 1 15 192 12 191 5.45e-58 181
MS.gene39163.t1 AT5G42500 52.349 149 71 0 43 191 37 185 2.17e-56 176
MS.gene39163.t1 AT1G22900 49.143 175 84 2 17 191 24 193 6.36e-56 175
MS.gene39163.t1 AT5G42510 51.007 149 73 0 43 191 34 182 1.19e-54 172
MS.gene39163.t1 AT3G13650 47.179 195 91 6 1 192 1 186 8.52e-52 165
MS.gene39163.t1 AT3G13662 53.103 145 65 2 33 177 27 168 3.68e-51 162
MS.gene39163.t1 AT2G21100 41.969 193 104 3 1 191 1 187 1.65e-49 159
MS.gene39163.t1 AT2G21100 41.969 193 104 3 1 191 1 187 1.65e-49 159
MS.gene39163.t1 AT5G49030 46.821 173 92 0 15 187 12 184 3.24e-48 168
MS.gene39163.t1 AT3G13660 58.120 117 47 1 78 192 9 125 7.04e-43 140
MS.gene39163.t1 AT4G38700 38.298 188 104 6 14 192 6 190 5.37e-40 135
MS.gene39163.t1 AT2G21110 42.949 156 81 3 45 192 31 186 5.87e-38 129
MS.gene39163.t1 AT3G58090 55.963 109 46 1 85 191 163 271 7.92e-37 129
MS.gene39163.t1 AT3G24020 28.889 135 87 3 61 189 110 241 7.85e-13 65.1
MS.gene39163.t1 AT4G13580 28.889 135 87 3 61 189 111 242 3.05e-12 63.5
MS.gene39163.t1 AT4G11210 26.471 170 110 5 15 177 5 166 4.82e-12 62.0
MS.gene39163.t1 AT4G11190 28.571 140 93 3 36 169 20 158 2.69e-11 60.1

Find 56 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGTAATCTCAGTGTTT+TGG 0.259860 1.2:-41926802 MS.gene39163:CDS
TGTGGAGTCCTAGGGTTGTT+GGG 0.287660 1.2:+41927096 None:intergenic
AGGAAGTAGATGAGGGATAA+GGG 0.337506 1.2:+41927173 None:intergenic
TCCTAGGGTTGTTGGGGAGA+TGG 0.354171 1.2:+41927103 None:intergenic
GAGGAAGTAGATGAGGGATA+AGG 0.362909 1.2:+41927172 None:intergenic
GACTCCGGACGCGCTGTTAA+CGG 0.362933 1.2:+41926933 None:intergenic
ACTCCGGACGCGCTGTTAAC+GGG 0.366524 1.2:+41926934 None:intergenic
TTGTGGAGTCCTAGGGTTGT+TGG 0.373760 1.2:+41927095 None:intergenic
TTTGAGGTTTGTGGAGTCCT+AGG 0.379817 1.2:+41927087 None:intergenic
ATGCCGGTTGTGGGTGGTAG+TGG 0.389118 1.2:-41926747 MS.gene39163:CDS
TTCACACGAGGGAAGTATAA+TGG 0.406207 1.2:-41926822 MS.gene39163:CDS
AGGCGACCCGGACAGCAGTT+TGG 0.419537 1.2:+41927015 None:intergenic
GGATGTTGAGAAGAAAAGTT+TGG 0.430943 1.2:+41927199 None:intergenic
CGACCCGTTAACAGCGCGTC+CGG 0.452836 1.2:-41926937 MS.gene39163:CDS
CGTGAGAGAGATGCCGGTTG+TGG 0.463566 1.2:-41926757 MS.gene39163:CDS
AACCCACTACCACCCACAAC+CGG 0.476541 1.2:+41926744 None:intergenic
TTGAGGTTTGTGGAGTCCTA+GGG 0.486598 1.2:+41927088 None:intergenic
GTGGAGTCCTAGGGTTGTTG+GGG 0.490912 1.2:+41927097 None:intergenic
TGGGTTGTTCAGATTTGCAA+GGG 0.492504 1.2:-41926727 MS.gene39163:CDS
AGTAGATGAGGGATAAGGGA+AGG 0.507437 1.2:+41927177 None:intergenic
TTGTGCTCTTCCCACAACTT+CGG 0.510733 1.2:+41926905 None:intergenic
CGTGGAAGTAGAAGCGGATG+TGG 0.512512 1.2:+41927057 None:intergenic
TGCCGGTTGTGGGTGGTAGT+GGG 0.514800 1.2:-41926746 MS.gene39163:CDS
GATGAAGAGGAAGTAGATGA+GGG 0.521113 1.2:+41927166 None:intergenic
TCAGCTTCACAGAGTGAAGT+GGG 0.523071 1.2:-41926870 MS.gene39163:CDS
TTCTACTTCCACGACATCAT+CGG 0.523779 1.2:-41927047 MS.gene39163:CDS
GTGGGTTGTTCAGATTTGCA+AGG 0.530567 1.2:-41926728 MS.gene39163:CDS
GTGAGAGAGATGCCGGTTGT+GGG 0.540631 1.2:-41926756 MS.gene39163:CDS
ACCATCTCCCCAACAACCCT+AGG 0.552762 1.2:-41927104 MS.gene39163:CDS
AAATGCATTAGAAGCTATTG+TGG 0.554492 1.2:-41926676 MS.gene39163:CDS
GAGTGAAGTGGGGTTTCTCA+TGG 0.555374 1.2:-41926859 MS.gene39163:CDS
TGATGAAGAGGAAGTAGATG+AGG 0.561084 1.2:+41927165 None:intergenic
TGGTTGGAGATGAGGCGACC+CGG 0.565807 1.2:+41927003 None:intergenic
GTAGATGAGGGATAAGGGAA+GGG 0.571545 1.2:+41927178 None:intergenic
ATCCGCCGTGAGAGAGATGC+CGG 0.574343 1.2:-41926763 MS.gene39163:CDS
CTTAACTTTGCATTCACACG+AGG 0.574668 1.2:-41926834 MS.gene39163:CDS
GGAGTCAAACTCCGAAGTTG+TGG 0.581001 1.2:-41926916 MS.gene39163:CDS
TGGTAATGGTGGTGATGAAG+AGG 0.589837 1.2:+41927153 None:intergenic
ACAACTTCGGAGTTTGACTC+CGG 0.593268 1.2:+41926918 None:intergenic
GAGTCAAACTCCGAAGTTGT+GGG 0.593363 1.2:-41926915 MS.gene39163:CDS
GAAGTTGTGGGAAGAGCACA+AGG 0.595582 1.2:-41926903 MS.gene39163:CDS
AACCACTGAACCAAAACCCG+TGG 0.598362 1.2:+41926968 None:intergenic
ACCACTGAACCAAAACCCGT+GGG 0.602599 1.2:+41926969 None:intergenic
TTCAGCTTCACAGAGTGAAG+TGG 0.609451 1.2:-41926871 MS.gene39163:CDS
CACAACCGGCATCTCTCTCA+CGG 0.612074 1.2:+41926758 None:intergenic
TACTTCCACGACATCATCGG+TGG 0.615547 1.2:-41927044 MS.gene39163:CDS
CACTGAACCAAAACCCGTGG+GGG 0.622706 1.2:+41926971 None:intergenic
TGATGTCGTGGAAGTAGAAG+CGG 0.627685 1.2:+41927051 None:intergenic
CAGCTTCACAGAGTGAAGTG+GGG 0.638369 1.2:-41926869 MS.gene39163:CDS
TGAGAAGAAAAGTTTGGCCA+TGG 0.638924 1.2:+41927205 None:intergenic
AGAGAGATGCCGGTTGTGGG+TGG 0.647672 1.2:-41926753 MS.gene39163:CDS
GGGTTGTTCAGATTTGCAAG+GGG 0.683015 1.2:-41926726 MS.gene39163:CDS
GGTTGTTCAGATTTGCAAGG+GGG 0.683578 1.2:-41926725 MS.gene39163:CDS
CCACTGAACCAAAACCCGTG+GGG 0.704423 1.2:+41926970 None:intergenic
TTAACTTTGCATTCACACGA+GGG 0.712435 1.2:-41926833 MS.gene39163:CDS
AACCGGCATCTCTCTCACGG+CGG 0.738069 1.2:+41926761 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATGGTTTTTATGAAAGAA+TGG + chr1.2:41926733-41926752 None:intergenic 25.0%
!! ATGGTTTTTATGAAAGAATG+GGG + chr1.2:41926731-41926750 None:intergenic 25.0%
!! GATGGTTTTTATGAAAGAAT+GGG + chr1.2:41926732-41926751 None:intergenic 25.0%
AAATGCATTAGAAGCTATTG+TGG - chr1.2:41927174-41927193 MS.gene39163:CDS 30.0%
GGATGTTGAGAAGAAAAGTT+TGG + chr1.2:41926654-41926673 None:intergenic 35.0%
TTAACTTTGCATTCACACGA+GGG - chr1.2:41927017-41927036 MS.gene39163:CDS 35.0%
! GGAAGTAATCTCAGTGTTTT+GGG - chr1.2:41927049-41927068 MS.gene39163:CDS 35.0%
! TGGAAGTAATCTCAGTGTTT+TGG - chr1.2:41927048-41927067 MS.gene39163:CDS 35.0%
!!! TTTTGGTTCAGTGGTTATGA+TGG - chr1.2:41926889-41926908 MS.gene39163:CDS 35.0%
AAAAAACCAAACTGCTGTCC+GGG - chr1.2:41926829-41926848 MS.gene39163:CDS 40.0%
AGGAAGTAGATGAGGGATAA+GGG + chr1.2:41926680-41926699 None:intergenic 40.0%
CTTAACTTTGCATTCACACG+AGG - chr1.2:41927016-41927035 MS.gene39163:CDS 40.0%
GAAAAAACCAAACTGCTGTC+CGG - chr1.2:41926828-41926847 MS.gene39163:CDS 40.0%
GATGAAGAGGAAGTAGATGA+GGG + chr1.2:41926687-41926706 None:intergenic 40.0%
TGATGAAGAGGAAGTAGATG+AGG + chr1.2:41926688-41926707 None:intergenic 40.0%
TGGGTTGTTCAGATTTGCAA+GGG - chr1.2:41927123-41927142 MS.gene39163:CDS 40.0%
TTCTACTTCCACGACATCAT+CGG - chr1.2:41926803-41926822 MS.gene39163:CDS 40.0%
! TTCACACGAGGGAAGTATAA+TGG - chr1.2:41927028-41927047 MS.gene39163:CDS 40.0%
!! TTTTTCAGTGGTTGGAGATG+AGG + chr1.2:41926858-41926877 None:intergenic 40.0%
!!! GGCTTAGTTTTTGAGGTTTG+TGG + chr1.2:41926775-41926794 None:intergenic 40.0%
!!! GTAATCTCAGTGTTTTGGGA+AGG - chr1.2:41927053-41927072 MS.gene39163:CDS 40.0%
AACCACTGAAAAATCCCCCA+CGG - chr1.2:41926865-41926884 MS.gene39163:CDS 45.0%
ACAACTTCGGAGTTTGACTC+CGG + chr1.2:41926935-41926954 None:intergenic 45.0%
AGTAGATGAGGGATAAGGGA+AGG + chr1.2:41926676-41926695 None:intergenic 45.0%
GAGGAAGTAGATGAGGGATA+AGG + chr1.2:41926681-41926700 None:intergenic 45.0%
GAGTCAAACTCCGAAGTTGT+GGG - chr1.2:41926935-41926954 MS.gene39163:CDS 45.0%
GGGTTGTTCAGATTTGCAAG+GGG - chr1.2:41927124-41927143 MS.gene39163:CDS 45.0%
GGTTGTTCAGATTTGCAAGG+GGG - chr1.2:41927125-41927144 MS.gene39163:CDS 45.0%
GTAGATGAGGGATAAGGGAA+GGG + chr1.2:41926675-41926694 None:intergenic 45.0%
TCAGCTTCACAGAGTGAAGT+GGG - chr1.2:41926980-41926999 MS.gene39163:CDS 45.0%
TGATGTCGTGGAAGTAGAAG+CGG + chr1.2:41926802-41926821 None:intergenic 45.0%
TTCAGCTTCACAGAGTGAAG+TGG - chr1.2:41926979-41926998 MS.gene39163:CDS 45.0%
TTGAGGTTTGTGGAGTCCTA+GGG + chr1.2:41926765-41926784 None:intergenic 45.0%
TTGTGCTCTTCCCACAACTT+CGG + chr1.2:41926948-41926967 None:intergenic 45.0%
! GAAAGAATGGGGTTTTGCTG+TGG + chr1.2:41926720-41926739 None:intergenic 45.0%
! GTGGGTTGTTCAGATTTGCA+AGG - chr1.2:41927122-41927141 MS.gene39163:CDS 45.0%
! TTTGAGGTTTGTGGAGTCCT+AGG + chr1.2:41926766-41926785 None:intergenic 45.0%
! TTTTTCCACCGATGATGTCG+TGG + chr1.2:41926814-41926833 None:intergenic 45.0%
!! ATGGGGTTTTGCTGTGGTAA+TGG + chr1.2:41926714-41926733 None:intergenic 45.0%
!! CGGATGTGGCTTAGTTTTTG+AGG + chr1.2:41926782-41926801 None:intergenic 45.0%
!! TGGTAATGGTGGTGATGAAG+AGG + chr1.2:41926700-41926719 None:intergenic 45.0%
AACCACTGAACCAAAACCCG+TGG + chr1.2:41926885-41926904 None:intergenic 50.0%
ACCACTGAAAAATCCCCCAC+GGG - chr1.2:41926866-41926885 MS.gene39163:CDS 50.0%
ACCACTGAACCAAAACCCGT+GGG + chr1.2:41926884-41926903 None:intergenic 50.0%
CAGCTTCACAGAGTGAAGTG+GGG - chr1.2:41926981-41927000 MS.gene39163:CDS 50.0%
GAGTGAAGTGGGGTTTCTCA+TGG - chr1.2:41926991-41927010 MS.gene39163:CDS 50.0%
GGAGTCAAACTCCGAAGTTG+TGG - chr1.2:41926934-41926953 MS.gene39163:CDS 50.0%
TACTTCCACGACATCATCGG+TGG - chr1.2:41926806-41926825 MS.gene39163:CDS 50.0%
TGTGGAGTCCTAGGGTTGTT+GGG + chr1.2:41926757-41926776 None:intergenic 50.0%
TTGTGGAGTCCTAGGGTTGT+TGG + chr1.2:41926758-41926777 None:intergenic 50.0%
! GAAAAATCCCCCACGGGTTT+TGG - chr1.2:41926872-41926891 MS.gene39163:CDS 50.0%
! GAAGTTGTGGGAAGAGCACA+AGG - chr1.2:41926947-41926966 MS.gene39163:CDS 50.0%
!! GTGGGGGATTTTTCAGTGGT+TGG + chr1.2:41926866-41926885 None:intergenic 50.0%
!!! GGGTTTTGCTGTGGTAATGG+TGG + chr1.2:41926711-41926730 None:intergenic 50.0%
AACCCACTACCACCCACAAC+CGG + chr1.2:41927109-41927128 None:intergenic 55.0%
ACCATCTCCCCAACAACCCT+AGG - chr1.2:41926746-41926765 MS.gene39163:CDS 55.0%
CACAACCGGCATCTCTCTCA+CGG + chr1.2:41927095-41927114 None:intergenic 55.0%
CACTGAACCAAAACCCGTGG+GGG + chr1.2:41926882-41926901 None:intergenic 55.0%
CCACTGAACCAAAACCCGTG+GGG + chr1.2:41926883-41926902 None:intergenic 55.0%
CGTGGAAGTAGAAGCGGATG+TGG + chr1.2:41926796-41926815 None:intergenic 55.0%
GTGGAGTCCTAGGGTTGTTG+GGG + chr1.2:41926756-41926775 None:intergenic 55.0%
TCCTAGGGTTGTTGGGGAGA+TGG + chr1.2:41926750-41926769 None:intergenic 55.0%
! GTGAGAGAGATGCCGGTTGT+GGG - chr1.2:41927094-41927113 MS.gene39163:CDS 55.0%
!! ACCCGTGGGGGATTTTTCAG+TGG + chr1.2:41926870-41926889 None:intergenic 55.0%
AACCGGCATCTCTCTCACGG+CGG + chr1.2:41927092-41927111 None:intergenic 60.0%
ACTCCGGACGCGCTGTTAAC+GGG + chr1.2:41926919-41926938 None:intergenic 60.0%
ATCCGCCGTGAGAGAGATGC+CGG - chr1.2:41927087-41927106 MS.gene39163:CDS 60.0%
GACTCCGGACGCGCTGTTAA+CGG + chr1.2:41926920-41926939 None:intergenic 60.0%
TGGTTGGAGATGAGGCGACC+CGG + chr1.2:41926850-41926869 None:intergenic 60.0%
! AGAGAGATGCCGGTTGTGGG+TGG - chr1.2:41927097-41927116 MS.gene39163:CDS 60.0%
! ATGCCGGTTGTGGGTGGTAG+TGG - chr1.2:41927103-41927122 MS.gene39163:CDS 60.0%
! CGTGAGAGAGATGCCGGTTG+TGG - chr1.2:41927093-41927112 MS.gene39163:CDS 60.0%
! TGCCGGTTGTGGGTGGTAGT+GGG - chr1.2:41927104-41927123 MS.gene39163:CDS 60.0%
!!! CCCCACGGGTTTTGGTTCAG+TGG - chr1.2:41926880-41926899 MS.gene39163:CDS 60.0%
AGGCGACCCGGACAGCAGTT+TGG + chr1.2:41926838-41926857 None:intergenic 65.0%
CGACCCGTTAACAGCGCGTC+CGG - chr1.2:41926913-41926932 MS.gene39163:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 41926647 41927225 41926647 ID=MS.gene39163
chr1.2 mRNA 41926647 41927225 41926647 ID=MS.gene39163.t1;Parent=MS.gene39163
chr1.2 exon 41926647 41927225 41926647 ID=MS.gene39163.t1.exon1;Parent=MS.gene39163.t1
chr1.2 CDS 41926647 41927225 41926647 ID=cds.MS.gene39163.t1;Parent=MS.gene39163.t1
Gene Sequence

>MS.gene39163

ATGGCCAAACTTTTCTTCTCAACATCCCTTCCCTTATCCCTCATCTACTTCCTCTTCATCACCACCATTACCACAGCAAAACCCCATTCTTTCATAAAAACCATCTCCCCAACAACCCTAGGACTCCACAAACCTCAAAAACTAAGCCACATCCGCTTCTACTTCCACGACATCATCGGTGGAAAAAACCAAACTGCTGTCCGGGTCGCCTCATCTCCAACCACTGAAAAATCCCCCACGGGTTTTGGTTCAGTGGTTATGATGGACGACCCGTTAACAGCGCGTCCGGAGTCAAACTCCGAAGTTGTGGGAAGAGCACAAGGAATCTATGCTTCAGCTTCACAGAGTGAAGTGGGGTTTCTCATGGTGCTTAACTTTGCATTCACACGAGGGAAGTATAATGGAAGTAATCTCAGTGTTTTGGGAAGGAATACGATCGAATCCGCCGTGAGAGAGATGCCGGTTGTGGGTGGTAGTGGGTTGTTCAGATTTGCAAGGGGGTATGCTCAAGCTTCAACTCATTCTATAAATGCATTAGAAGCTATTGTGGAGTATAATGTTTATGTGTTTCATTATTAA
Protein sequence

>MS.gene39163.t1

MAKLFFSTSLPLSLIYFLFITTITTAKPHSFIKTISPTTLGLHKPQKLSHIRFYFHDIIGGKNQTAVRVASSPTTEKSPTGFGSVVMMDDPLTARPESNSEVVGRAQGIYASASQSEVGFLMVLNFAFTRGKYNGSNLSVLGRNTIESAVREMPVVGGSGLFRFARGYAQASTHSINALEAIVEYNVYVFHY