AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40397

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40397.t1 MTR_7g095080 96.828 725 22 1 1 724 1 725 0.0 1457
MS.gene40397.t1 MTR_4g094992 92.664 518 32 1 213 724 2 519 0.0 994
MS.gene40397.t1 MTR_1g023985 46.423 685 309 5 41 724 563 1190 0.0 637
MS.gene40397.t1 MTR_1g114490 45.877 667 303 6 59 724 512 1121 0.0 612
MS.gene40397.t1 MTR_3g023880 45.109 685 316 8 43 724 407 1034 0.0 596
MS.gene40397.t1 MTR_6g061840 44.526 685 319 7 41 720 247 875 0.0 586
MS.gene40397.t1 MTR_2g015320 40.993 705 339 7 41 723 46 695 0.0 558
MS.gene40397.t1 MTR_1g086640 41.667 720 346 8 22 724 31 693 0.0 553
MS.gene40397.t1 MTR_5g075310 42.291 681 293 6 43 720 204 787 0.0 548
MS.gene40397.t1 MTR_3g052610 40.162 742 339 11 27 722 33 715 3.56e-178 526
MS.gene40397.t1 MTR_3g054400 40.435 690 345 8 41 722 361 992 1.08e-177 536
MS.gene40397.t1 MTR_2g098440 40.232 691 347 8 41 723 564 1196 1.16e-174 534
MS.gene40397.t1 MTR_6g061840 46.410 571 245 7 41 606 247 761 2.75e-169 505
MS.gene40397.t1 MTR_4g485610 40.361 664 322 8 78 724 49 655 1.04e-167 498
MS.gene40397.t1 MTR_8g080240 38.081 688 345 14 47 712 3 631 1.49e-153 461
MS.gene40397.t1 MTR_8g080240 38.081 688 345 14 47 712 3 631 3.32e-153 461
MS.gene40397.t1 MTR_8g080240 38.295 645 321 13 47 673 3 588 1.36e-142 430
MS.gene40397.t1 MTR_8g041990 36.157 661 251 10 43 699 101 594 2.29e-126 390
MS.gene40397.t1 MTR_6g061840 49.869 381 150 1 41 421 247 586 8.47e-124 382
MS.gene40397.t1 MTR_3g086770 32.773 714 374 20 41 717 255 899 8.97e-96 326
MS.gene40397.t1 MTR_3g014030 32.113 710 384 18 41 717 255 899 3.77e-95 324
MS.gene40397.t1 MTR_0267s0070 32.650 683 364 18 66 717 937 1554 2.55e-90 310
MS.gene40397.t1 MTR_1g056490 30.634 741 428 18 28 712 211 921 1.41e-78 271
MS.gene40397.t1 MTR_8g106630 29.195 733 433 19 35 709 260 964 2.51e-78 273
MS.gene40397.t1 MTR_1543s0010 40.836 311 164 5 327 633 10 304 8.68e-69 228
MS.gene40397.t1 MTR_4g023590 37.500 328 164 8 15 320 227 535 5.87e-56 207
MS.gene40397.t1 MTR_4g023590 38.624 189 114 2 332 519 692 879 3.60e-32 134
MS.gene40397.t1 MTR_3g055020 41.202 233 121 3 385 616 44 261 2.01e-53 191
MS.gene40397.t1 MTR_8g045110 28.406 433 288 7 288 712 105 523 7.49e-48 179
MS.gene40397.t1 MTR_1g109170 29.493 434 273 11 292 709 601 1017 7.81e-48 183
MS.gene40397.t1 MTR_1g109170 34.959 246 108 4 35 273 316 516 3.57e-32 134
MS.gene40397.t1 MTR_1g109170 29.452 438 268 11 292 709 601 1017 8.31e-48 183
MS.gene40397.t1 MTR_1g109170 34.959 246 108 4 35 273 316 516 3.37e-32 134
MS.gene40397.t1 MTR_4g134790 30.162 494 267 16 280 707 888 1369 9.00e-44 171
MS.gene40397.t1 MTR_4g134790 33.808 281 131 6 7 273 562 801 3.04e-35 144
MS.gene40397.t1 MTR_4g134790 30.162 494 267 16 280 707 885 1366 9.40e-44 171
MS.gene40397.t1 MTR_4g134790 33.808 281 131 6 7 273 562 801 3.09e-35 144
MS.gene40397.t1 MTR_1g088590 29.124 491 247 15 48 491 30 466 1.05e-42 167
MS.gene40397.t1 MTR_8g044260 28.146 437 284 10 288 709 518 939 2.03e-41 163
MS.gene40397.t1 MTR_8g044260 31.600 250 119 3 27 269 228 432 8.46e-31 130
MS.gene40397.t1 MTR_5g090430 28.186 408 253 9 273 669 413 791 7.27e-40 158
MS.gene40397.t1 MTR_5g090430 29.752 242 115 6 45 273 71 270 6.58e-26 114
MS.gene40397.t1 MTR_5g090430 28.009 432 274 11 273 682 526 942 9.02e-40 158
MS.gene40397.t1 MTR_5g090430 29.752 242 115 6 45 273 184 383 5.93e-26 115
MS.gene40397.t1 MTR_4g120070 32.374 278 130 7 77 320 208 461 6.88e-33 137
MS.gene40397.t1 MTR_4g120070 37.624 202 119 3 324 519 605 805 9.95e-33 136
MS.gene40397.t1 MTR_3g054420 46.552 58 31 0 518 575 2 59 8.17e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40397.t1 AT1G26370 68.864 713 211 3 10 720 10 713 0.0 1024
MS.gene40397.t1 AT3G26560 46.715 685 307 7 41 724 508 1135 0.0 636
MS.gene40397.t1 AT2G35340 44.663 712 326 9 24 724 372 1026 0.0 610
MS.gene40397.t1 AT2G35340 44.663 712 326 9 24 724 234 888 0.0 609
MS.gene40397.t1 AT1G32490 44.196 715 338 9 13 724 360 1016 0.0 608
MS.gene40397.t1 AT1G32490 44.196 715 338 9 13 724 370 1026 0.0 607
MS.gene40397.t1 AT4G16680 42.897 711 341 8 19 724 106 756 0.0 590
MS.gene40397.t1 AT3G62310 42.559 719 339 8 23 724 36 697 0.0 564
MS.gene40397.t1 AT2G47250 42.238 715 339 8 27 724 44 701 0.0 560
MS.gene40397.t1 AT4G18465 40.086 701 344 7 41 720 41 686 0.0 545
MS.gene40397.t1 AT5G13010 40.462 692 346 8 41 724 551 1184 5.99e-178 542
MS.gene40397.t1 AT3G62310 45.009 571 250 8 14 562 25 553 4.85e-154 459
MS.gene40397.t1 AT3G62310 46.026 541 235 6 14 539 25 523 1.49e-153 457
MS.gene40397.t1 AT4G16680 48.211 475 201 3 19 493 186 615 4.67e-151 454
MS.gene40397.t1 AT1G27900 36.652 693 359 14 47 715 3 639 7.49e-149 450
MS.gene40397.t1 AT1G27900 38.240 591 300 9 47 628 3 537 8.86e-140 421
MS.gene40397.t1 AT1G27900 38.240 591 300 9 47 628 3 537 2.57e-139 421
MS.gene40397.t1 AT5G10370 31.337 718 395 16 38 723 296 947 1.85e-97 331
MS.gene40397.t1 AT4G01020 30.508 708 394 17 48 723 303 944 6.32e-89 306
MS.gene40397.t1 AT2G35920 31.163 722 415 19 45 712 167 860 9.31e-86 290
MS.gene40397.t1 AT2G35920 31.154 719 413 19 45 709 225 915 1.01e-85 291
MS.gene40397.t1 AT2G35920 31.154 719 413 19 45 709 257 947 2.06e-85 291
MS.gene40397.t1 AT2G01130 29.155 734 437 17 27 705 134 839 2.91e-80 277
MS.gene40397.t1 AT1G48650 29.719 747 447 19 16 709 267 988 3.03e-80 278
MS.gene40397.t1 AT1G48650 29.719 747 447 19 16 709 267 988 3.04e-80 279
MS.gene40397.t1 AT2G01130 29.155 734 437 17 27 705 199 904 6.06e-80 277
MS.gene40397.t1 AT1G58050 29.870 693 381 23 14 636 561 1218 5.41e-72 256
MS.gene40397.t1 AT1G33390 38.908 293 137 5 37 312 230 497 4.30e-52 196
MS.gene40397.t1 AT1G33390 41.791 201 103 3 331 524 646 839 3.49e-38 153
MS.gene40397.t1 AT1G33390 38.908 293 137 5 37 312 230 497 4.30e-52 196
MS.gene40397.t1 AT1G33390 41.791 201 103 3 331 524 646 839 3.49e-38 153
MS.gene40397.t1 AT5G14900 34.965 286 159 8 448 724 3 270 1.99e-49 176
MS.gene40397.t1 AT1G58060 30.426 493 266 15 280 707 895 1375 4.86e-47 181
MS.gene40397.t1 AT1G58060 35.081 248 111 6 37 273 603 811 5.69e-36 147
MS.gene40397.t1 AT1G06670 29.040 396 251 9 247 630 524 901 2.08e-41 164
MS.gene40397.t1 AT1G06670 33.333 240 114 3 39 273 208 406 6.98e-33 137
MS.gene40397.t1 AT1G06670 29.040 396 251 9 247 630 524 901 2.43e-41 163
MS.gene40397.t1 AT1G06670 33.333 240 114 3 39 273 208 406 7.05e-33 137
MS.gene40397.t1 AT2G30800 28.302 477 289 16 274 716 527 984 1.15e-39 158
MS.gene40397.t1 AT2G30800 32.083 240 117 4 39 273 178 376 9.82e-30 127
MS.gene40397.t1 AT2G30800 28.302 477 289 16 274 716 184 641 1.41e-39 157
MS.gene40397.t1 AT5G04895 28.046 435 283 11 288 707 529 948 5.41e-39 155
MS.gene40397.t1 AT5G04895 30.709 254 124 3 27 273 239 447 4.69e-32 134
MS.gene40397.t1 AT2G47680 29.888 445 228 12 47 456 25 420 4.62e-38 152

Find 150 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGGTGTTGATGACATATT+AGG 0.230492 7.2:+14629210 MS.gene40397:CDS
GCATCTACATAATCTGTTTC+TGG 0.232424 7.2:-14626371 None:intergenic
TAGAGCAGCTACTGATTTAT+TGG 0.232627 7.2:+14631061 MS.gene40397:CDS
GCCTTTGATCCGGCTCCATC+TGG 0.237514 7.2:+14626847 MS.gene40397:CDS
ATAAGAGCTTTGGCATAAAC+TGG 0.292906 7.2:-14630606 None:intergenic
TTGATTATAGTTGGTGAAAC+TGG 0.312675 7.2:+14625019 MS.gene40397:CDS
ATGAAAAGGAATTTAGAAAA+TGG 0.316581 7.2:+14631117 MS.gene40397:CDS
TTCTAAGAAAAGTGTCATTC+TGG 0.319203 7.2:-14626199 None:intergenic
TGTTCATAGGGAAGCGTTGC+TGG 0.323561 7.2:+14625989 MS.gene40397:intron
CACAGCTGCATTGAGGAAAA+AGG 0.328575 7.2:-14631376 None:intergenic
CTCGCGCGGAAAATAAAATA+TGG 0.329052 7.2:-14630694 None:intergenic
TTATGCACTCTGGTTTCTTC+CGG 0.338992 7.2:-14631705 None:intergenic
AGGTTGCAACGCTTAATTTC+CGG 0.339311 7.2:-14629159 None:intergenic
TTCATGTTGGATAAAGATAA+TGG 0.353602 7.2:+14626176 MS.gene40397:CDS
TCTTTGCAATGCCTGGGACT+TGG 0.361472 7.2:-14628252 None:intergenic
AAGTCACTTCCTATTGCTTC+CGG 0.367651 7.2:+14624871 MS.gene40397:CDS
CCCTTGAACGTGAACAGCTT+TGG 0.383415 7.2:-14626312 None:intergenic
TAACAGAGTACCCAACCTTT+TGG 0.385983 7.2:-14625605 None:intergenic
TTCTTTACACAACAGGGCTT+TGG 0.389466 7.2:+14631639 MS.gene40397:intron
GAGACATCAATTACAATTCC+TGG 0.391735 7.2:+14628140 MS.gene40397:CDS
CATACCTTCTTTGCAATGCC+TGG 0.393486 7.2:-14628259 None:intergenic
TGTCCACACTGATGTCTTGA+TGG 0.399891 7.2:+14626067 MS.gene40397:CDS
GATGGAAGAGCTGCATATAT+AGG 0.404918 7.2:-14626808 None:intergenic
TGGGTGTTGATGACATATTA+GGG 0.411725 7.2:+14629211 MS.gene40397:CDS
TCAAGGACATGTTCAACAAA+TGG 0.413696 7.2:+14631293 MS.gene40397:CDS
TCAATTACATACTTGATTCC+AGG 0.417163 7.2:-14628158 None:intergenic
AGCAAGTCAGTTTAACTGCT+TGG 0.419602 7.2:+14630632 MS.gene40397:CDS
AGAGATTAATTGAAGAGGTT+CGG 0.420010 7.2:+14624984 MS.gene40397:CDS
ATTGGTGTTGGCATTAGCAT+TGG 0.423696 7.2:-14624648 None:intergenic
GGCAAACAACTCATGGTGTT+TGG 0.423711 7.2:+14624578 None:intergenic
GCCAAAGCTGTTCACGTTCA+AGG 0.430027 7.2:+14626311 MS.gene40397:CDS
GACAGCTATTATAAAATCAT+TGG 0.430046 7.2:+14630497 MS.gene40397:CDS
CGGGCCATTTGACGTCCAAC+TGG 0.430443 7.2:-14630570 None:intergenic
CAACCTATGGTTAACCGAAC+TGG 0.433171 7.2:+14631786 MS.gene40397:CDS
AACAATTATAACAGAATACT+TGG 0.433600 7.2:-14626024 None:intergenic
TAACCAGAGTTGACAACCTA+TGG 0.434887 7.2:+14631773 MS.gene40397:CDS
ACTTGCTAAAATAAGAGCTT+TGG 0.436656 7.2:-14630616 None:intergenic
ATCCTTCGTCTGTATTGTTC+CGG 0.437198 7.2:+14631686 MS.gene40397:CDS
TTTGGTAACAGTGGGCGTGC+AGG 0.437226 7.2:+14629063 MS.gene40397:intron
CCTTTGATCCGGCTCCATCT+GGG 0.438053 7.2:+14626848 MS.gene40397:CDS
TGACAAGGTTCTCACCTTGA+AGG 0.442041 7.2:-14629252 None:intergenic
AAGGACCTGGAAAATGCTTT+CGG 0.443930 7.2:+14629091 MS.gene40397:CDS
GAAGAATGTGGTTGTGAGTT+GGG 0.445363 7.2:+14625582 MS.gene40397:CDS
AACTGGGTCCAAGGGAAGTC+GGG 0.447934 7.2:-14630589 None:intergenic
GACCTGGAAAATTGTTATCA+AGG 0.449776 7.2:-14626396 None:intergenic
GCCACGACGGAAAAGTTGTT+GGG 0.450132 7.2:+14625508 MS.gene40397:CDS
TATTCTTGTTCTCATTATCA+AGG 0.450410 7.2:-14626145 None:intergenic
AATTCAATACCGGAAGCAAT+AGG 0.452539 7.2:-14624880 None:intergenic
CAGGGCTTTGGCAAGTGGTC+AGG 0.455322 7.2:+14631651 MS.gene40397:intron
GACATGCTCGTGACATTCAC+AGG 0.455685 7.2:+14631174 MS.gene40397:CDS
CGTGCAGGGCGTGAAGGACC+TGG 0.456345 7.2:+14629078 MS.gene40397:CDS
GTGAGTTGGGCCAAAAGGTT+GGG 0.456369 7.2:+14625595 MS.gene40397:CDS
ATCAAGTATGTAATTGATCC+TGG 0.458843 7.2:+14628164 MS.gene40397:CDS
AGTCCCAGGCATTGCAAAGA+AGG 0.461418 7.2:+14628255 MS.gene40397:CDS
CGTACTTTCTCTGAGTACTT+TGG 0.462297 7.2:+14626284 MS.gene40397:CDS
ACCGGGGAGAGGAATGGGTT+TGG 0.463561 7.2:-14624679 None:intergenic
TAACCATAGGTTGTCAACTC+TGG 0.467320 7.2:-14631776 None:intergenic
TGATCCAGTTGGACGTCAAA+TGG 0.470663 7.2:+14630566 MS.gene40397:CDS
TTGGTTATTCCGATTGGTGT+TGG 0.471136 7.2:-14624660 None:intergenic
TGCCACGACGGAAAAGTTGT+TGG 0.473901 7.2:+14625507 MS.gene40397:CDS
TGCTGTTGAACGGCTTATCA+AGG 0.477511 7.2:+14626738 MS.gene40397:CDS
GCTGCAAAATGACATTAGAA+AGG 0.479362 7.2:-14629179 None:intergenic
GATATACTAGTATTTCTGAC+TGG 0.479761 7.2:+14626700 MS.gene40397:CDS
AGAGTGATGTGGTCTCCCTC+TGG 0.480341 7.2:-14631026 None:intergenic
TCAAATGGCCCGACTTCCCT+TGG 0.483015 7.2:+14630581 MS.gene40397:CDS
GGCTTTGGCAAGTGGTCAGG+TGG 0.485781 7.2:+14631654 MS.gene40397:CDS
CAAGGACATGTTCAACAAAT+GGG 0.487040 7.2:+14631294 MS.gene40397:CDS
GCTCGCATTTGCTGCTCAGA+TGG 0.488003 7.2:-14626826 None:intergenic
AAAGCGCGTTCCTATGATCC+TGG 0.491588 7.2:+14628194 MS.gene40397:CDS
TGAGAAGAGATTAATTGAAG+AGG 0.492285 7.2:+14624979 MS.gene40397:CDS
ATGGGTTTGGTTATTCCGAT+TGG 0.493362 7.2:-14624666 None:intergenic
TTGGTAACAGTGGGCGTGCA+GGG 0.495052 7.2:+14629064 MS.gene40397:intron
CGTTCCTATGATCCTGGAAA+AGG 0.496489 7.2:+14628200 MS.gene40397:CDS
ATGCTAATGCCAACACCAAT+CGG 0.500852 7.2:+14624651 MS.gene40397:CDS
ATTTCATTGAGAAACCTTCA+AGG 0.501014 7.2:+14629238 MS.gene40397:CDS
TTGGTGTTGGCATTAGCATT+GGG 0.506589 7.2:-14624647 None:intergenic
TAATTTCGACGAGCCACAAA+AGG 0.506645 7.2:-14624620 None:intergenic
GCAAATGCGAGCCTTTGATC+CGG 0.507905 7.2:+14626837 MS.gene40397:CDS
GTAATACCGGGGAGAGGAAT+GGG 0.508438 7.2:-14624684 None:intergenic
ACAGAATACTTGGAAAGATA+AGG 0.508465 7.2:-14626014 None:intergenic
TGTGAGTTGGGCCAAAAGGT+TGG 0.511660 7.2:+14625594 MS.gene40397:CDS
GGATACCTGTAGGTTCCATC+TGG 0.513099 7.2:-14631405 None:intergenic
CGGTCAAATTCTATCAAAGA+TGG 0.513129 7.2:+14626116 MS.gene40397:CDS
TGAAGAATGTGGTTGTGAGT+TGG 0.513598 7.2:+14625581 MS.gene40397:CDS
ATACCTTCTTTGCAATGCCT+GGG 0.513868 7.2:-14628258 None:intergenic
TCGTTAAATATTATGCACTC+TGG 0.514440 7.2:-14631715 None:intergenic
ACTTTCTCTGAGTACTTTGG+TGG 0.515214 7.2:+14626287 MS.gene40397:CDS
CCCAGATGGAGCCGGATCAA+AGG 0.518326 7.2:-14626848 None:intergenic
ATATTTGATCCTCGTTGAGT+TGG 0.522636 7.2:-14625646 None:intergenic
CACAACAGGGCTTTGGCAAG+TGG 0.525131 7.2:+14631646 MS.gene40397:intron
AGTAATACCGGGGAGAGGAA+TGG 0.526454 7.2:-14624685 None:intergenic
ACATTCTTCAGCAACTCGAT+TGG 0.535455 7.2:-14625568 None:intergenic
GGTCTGAAACTTGCTTCTTG+TGG 0.535848 7.2:+14631315 MS.gene40397:CDS
TGACAGATGGATTGCTTCTG+AGG 0.540103 7.2:+14625670 MS.gene40397:CDS
CCAAAGCTGTTCACGTTCAA+GGG 0.541798 7.2:+14626312 MS.gene40397:CDS
ATAGTTGGTGAAACTGGAAG+TGG 0.542916 7.2:+14625025 MS.gene40397:CDS
GTGATAGCAGCAACACGTCT+AGG 0.544134 7.2:-14625540 None:intergenic
ACTTGAGGATTCTACAATGC+CGG 0.554792 7.2:+14629140 MS.gene40397:CDS
GAGCCAGTTCGGTTAACCAT+AGG 0.557137 7.2:-14631789 None:intergenic
TTGCGAAACCCAGATGGAGC+CGG 0.557730 7.2:-14626856 None:intergenic
CGAGTATAAAATATATCCAC+AGG 0.558690 7.2:-14626344 None:intergenic
AGGATCAAATATATGACAGA+TGG 0.560005 7.2:+14625657 MS.gene40397:CDS
GTAAAACTGAGGAGCCAGTT+CGG 0.560736 7.2:-14631800 None:intergenic
AAACTGGGTCCAAGGGAAGT+CGG 0.561694 7.2:-14630590 None:intergenic
AAATGAATTTGAGAAACTTG+AGG 0.562155 7.2:+14629125 MS.gene40397:CDS
TGGTTGTGAGTTGGGCCAAA+AGG 0.566424 7.2:+14625590 MS.gene40397:CDS
AAGAGCTTCGCAAGTCCAGA+GGG 0.566562 7.2:+14631011 MS.gene40397:CDS
GAATACGAATGGTTTCATGT+TGG 0.567599 7.2:+14626163 MS.gene40397:CDS
GGCTCCATCTGGGTTTCGCA+AGG 0.567724 7.2:+14626858 MS.gene40397:CDS
GACATGATGATTAACTTCAA+CGG 0.568214 7.2:-14626245 None:intergenic
AGTGGGCGTGCAGGGCGTGA+AGG 0.569161 7.2:+14629072 MS.gene40397:intron
CTATGATCCTGGAAAAGGCA+TGG 0.570476 7.2:+14628205 MS.gene40397:CDS
ACCAAACCCATTCCTCTCCC+CGG 0.570805 7.2:+14624678 MS.gene40397:CDS
ATATACTAGTATTTCTGACT+GGG 0.570954 7.2:+14626701 MS.gene40397:CDS
GGAACAATACAGACGAAGGA+TGG 0.575517 7.2:-14631684 None:intergenic
GGAAAAGTAATACCGGGGAG+AGG 0.581717 7.2:-14624690 None:intergenic
GCAGCTGTGAAGCAGCCAGA+TGG 0.589847 7.2:+14631390 MS.gene40397:CDS
AAGAGATTGATGCTGTTGAA+CGG 0.590120 7.2:+14626728 MS.gene40397:CDS
GTCCACACTGATGTCTTGAT+GGG 0.592239 7.2:+14626068 MS.gene40397:CDS
AATGAGAACAAGAATACGAA+TGG 0.596334 7.2:+14626152 MS.gene40397:CDS
TTGGCATAAACTGGGTCCAA+GGG 0.596569 7.2:-14630597 None:intergenic
GCTGCTTCACAGCTGCATTG+AGG 0.605522 7.2:-14631383 None:intergenic
GATACCTTGCGAAACCCAGA+TGG 0.606659 7.2:-14626862 None:intergenic
AGCAGCCAGATGGAACCTAC+AGG 0.608253 7.2:+14631400 MS.gene40397:CDS
TTTGGCATAAACTGGGTCCA+AGG 0.609632 7.2:-14630598 None:intergenic
CACGAGCATGTCTAAGAGAA+CGG 0.611248 7.2:-14631163 None:intergenic
CAAATTCTATCAAAGATGGT+CGG 0.611405 7.2:+14626120 MS.gene40397:CDS
TTCCGCAGAAAGCATTGCAA+CGG 0.612393 7.2:-14630670 None:intergenic
AGAAATACTAGTATATCACC+AGG 0.613029 7.2:-14626694 None:intergenic
AGTACCTACATCCAAGTCCC+AGG 0.615133 7.2:+14628241 MS.gene40397:CDS
GTACATCAAGATGAGGCCCC+TGG 0.615632 7.2:+14626676 MS.gene40397:CDS
TAAGAGCTTCGCAAGTCCAG+AGG 0.617178 7.2:+14631010 MS.gene40397:CDS
AGCCGTTGCAATGCTTTCTG+CGG 0.618592 7.2:+14630668 MS.gene40397:CDS
ACCTCTTCGTACTTCTCGCG+CGG 0.625786 7.2:-14630708 None:intergenic
TCCGCGCGAGAAGTACGAAG+AGG 0.633803 7.2:+14630707 MS.gene40397:CDS
TGTCAACTATCTGATCCAGT+TGG 0.635262 7.2:+14630555 MS.gene40397:CDS
AATGCCTGGGACTTGGATGT+AGG 0.635279 7.2:-14628245 None:intergenic
GTTATAGGTACATCAAGATG+AGG 0.635310 7.2:+14626669 MS.gene40397:intron
TAAGAGCTTTGGCATAAACT+GGG 0.636597 7.2:-14630605 None:intergenic
TGTCAACTCTGGTTAAATCA+CGG 0.646694 7.2:-14631765 None:intergenic
TTCCGGAACAATACAGACGA+AGG 0.647037 7.2:-14631688 None:intergenic
ACACATTAATCAGAGTGATG+TGG 0.651588 7.2:-14631037 None:intergenic
TCAAGGGAGACAGTTTCCTG+TGG 0.671336 7.2:+14626328 MS.gene40397:CDS
AATCGAGTTGCTGAAGAATG+TGG 0.673849 7.2:+14625570 MS.gene40397:CDS
GAAATACTAGTATATCACCA+GGG 0.680918 7.2:-14626693 None:intergenic
TTCTGCATAGCGTAAAACTG+AGG 0.692959 7.2:-14631811 None:intergenic
AAATACTAGTATATCACCAG+GGG 0.695525 7.2:-14626692 None:intergenic
ATTCTGCTTCCAACTCAACG+AGG 0.740341 7.2:+14625637 MS.gene40397:CDS
AGCCCATCAAGACATCAGTG+TGG 0.744619 7.2:-14626070 None:intergenic
ACGTGTTGCTGCTATCACTG+TGG 0.765431 7.2:+14625545 MS.gene40397:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.2 gene 14624590 14631837 14624590 ID=MS.gene40397
chr7.2 mRNA 14624590 14631837 14624590 ID=MS.gene40397.t1;Parent=MS.gene40397
chr7.2 exon 14624590 14624699 14624590 ID=MS.gene40397.t1.exon1;Parent=MS.gene40397.t1
chr7.2 CDS 14624590 14624699 14624590 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14624846 14624892 14624846 ID=MS.gene40397.t1.exon2;Parent=MS.gene40397.t1
chr7.2 CDS 14624846 14624892 14624846 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14624978 14625058 14624978 ID=MS.gene40397.t1.exon3;Parent=MS.gene40397.t1
chr7.2 CDS 14624978 14625058 14624978 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14625475 14625691 14625475 ID=MS.gene40397.t1.exon4;Parent=MS.gene40397.t1
chr7.2 CDS 14625475 14625691 14625475 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14625998 14626415 14625998 ID=MS.gene40397.t1.exon5;Parent=MS.gene40397.t1
chr7.2 CDS 14625998 14626415 14625998 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14626676 14626879 14626676 ID=MS.gene40397.t1.exon6;Parent=MS.gene40397.t1
chr7.2 CDS 14626676 14626879 14626676 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14628116 14628276 14628116 ID=MS.gene40397.t1.exon7;Parent=MS.gene40397.t1
chr7.2 CDS 14628116 14628276 14628116 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14629074 14629259 14629074 ID=MS.gene40397.t1.exon8;Parent=MS.gene40397.t1
chr7.2 CDS 14629074 14629259 14629074 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14630497 14630728 14630497 ID=MS.gene40397.t1.exon9;Parent=MS.gene40397.t1
chr7.2 CDS 14630497 14630728 14630497 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14630996 14631195 14630996 ID=MS.gene40397.t1.exon10;Parent=MS.gene40397.t1
chr7.2 CDS 14630996 14631195 14630996 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14631287 14631421 14631287 ID=MS.gene40397.t1.exon11;Parent=MS.gene40397.t1
chr7.2 CDS 14631287 14631421 14631287 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
chr7.2 exon 14631654 14631837 14631654 ID=MS.gene40397.t1.exon12;Parent=MS.gene40397.t1
chr7.2 CDS 14631654 14631837 14631654 ID=cds.MS.gene40397.t1;Parent=MS.gene40397.t1
Gene Sequence

>MS.gene40397

ATGGTGTTTGGTCAGAGAGATCGTAACAAGCCTTTTGTGGCTCGTCGAAATTATCAACCCAATGCTAATGCCAACACCAATCGGAATAACCAAACCCATTCCTCTCCCCGGTATTACTTTTCCTAATTCATTTCAATTTTCACTTTTTTTCAACTAAAATTAATCATGCTCAAAAGGGTAGCAGAAAAACACTAAATTTTGAATTGGGTTTTAGTTTTTTTTTTTGTTTGATTCTAATTTGTTAATTTTATTACAGGAGACAGGAGATTATAGAGCAGAGAAAGTCACTTCCTATTGCTTCCGGTATTGAATTTGTTTGTTTTTATGTGTTTTGAATGTTCTTCCTTTCATACATGACTTGTGACCGTTGGCATTGTTCTTATTGTAGTTGAGAAGAGATTAATTGAAGAGGTTCGGAAAAACGATATTTTGATTATAGTTGGTGAAACTGGAAGTGGAAAAACTACACGTAGGTTACATCTTATCCTATTAATCAACGTGTTTATGATCATATCGTTTATAATTTAGGGTATGTTTGGATTTACTTATTTGAGCTTATTTACTCGCATAAGGACTTGTGAGAATGTTTGAGAGTTTTTATGAAAACAACAAATAACATGTTCATAAGCTGTTTTCAGCAATTTCCATAAGTTCTCTAGGATAGCTTATGAAAACAGCTTATAGCTTATATGAAAACAATTTGTCTTTGTTTTATCTTTTGTTTTCAAAATAACTTACATTGTATGATAAGCGCTTATGTGATACACGCTTAATTTAGCTGTTTATTCAACCATGCCTTAGGTTTTTAGGAAAAGAAACGAAAAAAGGAATTTTAGAGATAGCAGGTTTTGACTGTGTTTATGTTTATTACATTGTAATGTGTAGAGATTCCACAATTTTTATTTCATGCTGGATTTTGCCACGACGGAAAAGTTGTTGGGATAACTCAGCCTAGACGTGTTGCTGCTATCACTGTGGCCAATCGAGTTGCTGAAGAATGTGGTTGTGAGTTGGGCCAAAAGGTTGGGTACTCTGTTAGATTTGATGATTCTGCTTCCAACTCAACGAGGATCAAATATATGACAGATGGATTGCTTCTGAGGTACAACTTTTTTGTAGCTTTGATTTATGGGCGTGTTTGTTTCCTATCAAGACATGATACTGTAATTTTTTTTTCATTCTTGATGTCAAACTATAAAAAATGCTATATCACTATTTAAGTCAATTGTATTCAACGACGGGTGAGTAACATCCCCTTCCCCCTCGTTGATGCGGGTTTGAGATAGTTACATGATTTGAAGTAGTTATGGAGGTTTCAGATTAAATGTTTTGTTTTTAATGGTTTGTATTTGTAAGTACTGTTTTTCTTATGCTAAAGTTAATAAGCATTTTTTGTTTGTGTTCATAGGGAAGCGTTGCTGGATCCTTATCTTTCCAAGTATTCTGTTATAATTGTTGATGAAGCACATGAGAGAACTGTCCACACTGATGTCTTGATGGGCTTGCTAAAAAATGTGCAACATGCTCGGTCAAATTCTATCAAAGATGGTCGGAACCTTGATAATGAGAACAAGAATACGAATGGTTTCATGTTGGATAAAGATAATGGCCAGAATGACACTTTTCTTAGAAAAGACCACCGCGAAAAGAGTTCTCCGTTGAAGTTAATCATCATGTCTGCAAGTCTTGATGCTCGTACTTTCTCTGAGTACTTTGGTGGTGCCAAAGCTGTTCACGTTCAAGGGAGACAGTTTCCTGTGGATATATTTTATACTCGTCATCCAGAAACAGATTATGTAGATGCTGCCTTGATAACAATTTTCCAGGTCATTGGTTTAATCAGTACATGTAATAATTTAATAACGTGGTTTAACTTATTTTTTTGTAAGAGACCTTAGATCCTAATAATATTTTTTTGGGACAGTGGTTTTTTGGTATGCATTTATGGTACATGTTTGTGCACTGCCTCAGTCATTCAGACATACTGCCACACACACACACACACAAGATTATAACATTGTGGTGATGGCTGAGAAGTTAAGTTCATATAATTGTCACTTAAAGTCATGTGTCTTTGATGTTATAGGTACATCAAGATGAGGCCCCTGGTGATATACTAGTATTTCTGACTGGGCAAGAAGAGATTGATGCTGTTGAACGGCTTATCAAGGAGCGACTTACAAAGTTACCTCAAGAAAATCAGAAGCTTCAAGTTGTGCCTATATATGCAGCTCTTCCATCTGAGCAGCAAATGCGAGCCTTTGATCCGGCTCCATCTGGGTTTCGCAAGGTATCCTGTAGCTATGCATATTATCCTTTGTTAAATTATCGTATTACTTCTGCGTAGAATTCAATAGAAAAATCAGGAAAATAAGTTTAATTTAACCAATCATTATTTTTTTATTTAATTTAAACAGTTGACATTTCCATCTAGGTTTTTATTTATTCTCTTGAAGTACTTTGTTTCCCATGACCATGTTAATTTATAAAGTAAATGCTGGTTCCTGATTGCCGCATTTAGTGCCTCAGCGTGTTTAGAACATATGCCCGTGCTTTGATATTTCATATTTTGTTATATGGAAGTTTCATCATTGGAAGTTCTCTTAGACACATATGACTTATATAATGATCATCTTCTGTTAGAAGTTAGAACTGGTATCGAAAGTAGAAAAAGTAAGATCGAAAAATGAAAATTGCAAAGGAGTGGTTCTTGAATTGATAACAATATGCACCCTGGCCAGTATATCATAGTGGTGGTGGATACACCAGCCTCGGGTTTCAAGTACCTGCAACTTCCAAATTCTACAATCCCCACAAACTCACCACTTTCTAGTTCCACCTGTATTTATAAGTTATAACTCCCTCTTCCTGTCTCTCCTTGATTAGTTATTCAGATGAAATGTATTTCCTATACTGTCCCCTGCTCTTGCTCATATCACTATAAATTACTAGAAGTTTCCTAACATCTTCCATTTACATTCATGATTTGTTACCTATAGTCTCTATCATTTGACAGTGTTGCCATTGTGGTCCAATTACTCTGATGTCAAATTTAGAACTAATTTTTTTTTCTTCCAATGTCATTATTTGCTTTGTTGCTTCTAACAGTGGATCACTTTGGTTGTAAAGTACTATTTGTATCGTTTTTATACCGTCATACTTACCGAAATGCACCTACCAGTATCTATGTATACCGACCTATTATTTTCCTTTACTTATAACCTTCATCGTGTGATATGTTACCATCACCTATACTCCTTTCGGAACTATGCCTTCTGAACTGGCAACAATCCCTATAAATAGTACATGGATATACAAAACAGTGTCTGCTAGGAGTAGGACCATATTTTTTTCCCATAGTAGAGTGTCACATTTTTAGTTTGAGCCTGTGAATAACAATAACACCAATCAAGCCTTTAATGTGAGCTAATTTATATTCAAATAACCTCTGATATTTAATATTTATCATAGCAGGTAATCGCAATTCTTAAATTGTATTCAACTTGGTACTTTGTTGTTTTGTTAGGTGGTATTGGCAACTAATATTGCTGAGACATCAATTACAATTCCTGGAATCAAGTATGTAATTGATCCTGGACTTGTAAAAGCGCGTTCCTATGATCCTGGAAAAGGCATGGAATCTTTGATTGTAGTACCTACATCCAAGTCCCAGGCATTGCAAAGAAGGTATGGTGCTGGATTATGTTTCAATCTGTTTAATATTTGATTCTGTTTGATGTTTAATTGCTGCTGCAATTGTTGGTTGAATTTAGTTAATGTAATGTAAATGAAGTAAGTTTGGTTCAGTTTAACTGAGGATGACTTCCTTTCTCATTTCAAGTTCAGTTTATTAATGGATACCTTTGATTTTCCTCAAAAACGTGTATTTTCTTAACAAAGGTTTTTGAAATGTGAATCTAGCATCATTATTTCTAGCACTTGTTTTTTGTTAAACATGGCGAGGCATAACAACAATTAACCAAAACCTTATCCCACTGGTTGTGGTCGGCTATGAGTCAAATGATGTCATAATTCAAATGACATCATAATCAAAACCATTGATTTCCCCCAAAAACATGTATTTTCTTAACAAAGACTTTTGGAACGTGAATCTAGCATCATTGTTTCTAGAATTTGTTTTTTATTACACATACCGAGGCATTACAACAACAATAATAAAACCTTATTCCACTAGTTGGGGTCGGCTATATGAGTCAAATGACGTCGTAGTTCCCTATCATGTGATAAGTTCAGAAATAGATTCTCTACTTCTAAATCTCTCTTGATGGTTTGGCCTATAGTTTTTCATGGTCTCACTCCACCTTTAACCATTGGACTGTCCTCTATCTAGCAAACTTTCCTTGACAGTGTCGTGTGTGTGCACTCGTCCATCTTAACCTTCTCATTATGTGACATAGGTAATATATTTAATTTGATTTTGCTATAAGAGTAGTGCAAATTGATTATCTATATTTTGGTAACAGTGGGCGTGCAGGGCGTGAAGGACCTGGAAAATGCTTTCGGCTATATCCAGAAAATGAATTTGAGAAACTTGAGGATTCTACAATGCCGGAAATTAAGCGTTGCAACCTTTCTAATGTCATTTTGCAGCTTAAGGCTTTGGGTGTTGATGACATATTAGGGTTCGATTTCATTGAGAAACCTTCAAGGTGAGAACCTTGTCAAAATTTTGGTTTGACAACTTCACTACCTATGCATATTTGTGGATAAGTTCTGATTGCTTATGTTTTTAAGGCTAAATTACTATGTAGATTTTAAAGTTTCACTTTGTTTTTAAATAGGTTCTTACACTTCAAAAAAATTAGTGTCTGTGATTCTAAAGATTTTTGTACGAGGGCCAACTACCAAGTACTATTAAGACAATGCAAGCTTTTGCTTAATTTGGAGACCCATTTGAAAGCATAGATAAGCTGAAGAGTTTGAGAGATATCACAGTTAAAAAGGCCCCTATACACTTAATCTTAATTTTCAAATAGGTCCTTATAAGCTACAATTTTGCTAAAGTTAACAGCCATTAGGGAGTAGGGATCTTATAAATTTGTCTAAACTATAAGAACCTATTTGAAAATTCATGAAACTATAGGGATCTGCAGACTAATGAAGACTATTTAAAATAAAATATGCATGTCCTGAAAGCTTTTTTTTCTAACTTCTGCTTATCTTTTAAAAGGATTTTAAGTCACTTGAAAAGATGCTATTATCTCTCTACTAAGATGATTCCTTCAACCTATTATAGGTCCTTTTGATTAACCTTTGATTTCTTTTTGTTGTAATGTGATCTAAACTGGAGATAAAAAATCCATTTGAATATTAGGTGCTTTAATTCAATTTTTCTTGGTCATGAAAAATAAAATTATTGAGGATTGACTATATTGTATTCATATACAGAAAGAATAAGAATGACATGACATTTGTATTGACAAATAACCAAAAATCTATAAAAGTGTCATATATATTATTAATGTAAAAGTACTTTTAGGAATACTTGTATACTGATGAAGAAACTGAGAAGAGTACATAGACAACACCTAATAAAACCTTGCTTGGACCAAGATCTTGCACCTTAAAATCAATCTATAAAGATAAATTTCTATGTACCTGAAAACTACAAAATATTGAAAGTGGGCTTTAGTGAATTTTTTTGGACTGAATGGAGAAAATAAAGGATGTAGATAATCTATTCCTTTGCATCAGTTATTAATAGTGAGTTCAAATATTTGAAACTTCTATTGATTCAGTTTCTTTAATGTCAGTTGTCTCGTCCATTGTACTCCAGAACTAAATTAACATTTTTTTAGAGTTCTTCGTTTCTTTCTTTATTTTCGAAAAGTAATATCACTGAGCAACATATTACTCAAAGCTTGTACCTGTCACATGTTTTAACAGGACAGCTATTATAAAATCATTGGAGCAGCTATTTTTGTTAGGTGCTTTGACAGATGACTGTCAACTATCTGATCCAGTTGGACGTCAAATGGCCCGACTTCCCTTGGACCCAGTTTATGCCAAAGCTCTTATTTTAGCAAGTCAGTTTAACTGCTTGGAAGAGATGTTGATAGCCGTTGCAATGCTTTCTGCGGAATCCATATTTTATTTTCCGCGCGAGAAGTACGAAGAGGTTTGAAGAGTGAATTCGGATTATAAACCATTCCTGTCATATTCTATTATTCTATTTCGGTGAAATGTAAATACGAGCATAAATCATTGATTATGTGCTATGCCACTCTATTGGTATATTCACCGTATAACAAAATATTTGAAAAATATCATACGGATGGTTTAACTATATGCGATTGGTAATTACCGTAGGAAGATTCTAGTCTTTATTTGACGTCAACTTGTCTTCTCTACACGAACTTATTGTGCTTCTCTGATTTATGGCCAGGCTAGAACTGCAGCTAAGAGCTTCGCAAGTCCAGAGGGAGACCACATCACTCTGATTAATGTGTATAGAGCAGCTACTGATTTATTGGAGAAGAGAACTATCGAAACTAATAAAACAAAAAATGAAAAGGAATTTAGAAAATGGTGCAAAGAAAATTTTATAAATAGCCGTTCTCTTAGACATGCTCGTGACATTCACAGGTTGTTTTATTGTGTTTAAACTTTACTTCAAGGCATCTATAATATCCTTGAGACATTTTGTGCATTCTAATTTCTGAAATGCCTTTTTCAGGCAGATTCAAGGACATGTTCAACAAATGGGTCTGAAACTTGCTTCTTGTGGAGAAGATATGCTTCAATTTCGCAGATGCCTTGTTGCTTCCTTTTTCCTCAATGCAGCTGTGAAGCAGCCAGATGGAACCTACAGGTATCCATTTTAACTTCAAAATTCTTGGGCAGTCCCGCTAGGGTGGCTTGCCATCTAATTGGAATCATTACATGAAAATATCGTTTAGTTTCGGGATTTTTCATTGGTAATTATGATTCGCTAATTGGAATCCTGACCTTACATGCATATACACTCTTCAAATATTTTGCTTTTTTGTTTTTACTTATACTTTTATGATAAAAAAGACTCTTACTGCTTCTTTACACAACAGGGCTTTGGCAAGTGGTCAGGTGGTGCAGATCCATCCTTCGTCTGTATTGTTCCGGAAGAAACCAGAGTGCATAATATTTAACGAACTGATTCAAACTAACAAAAAGTATGTCCGTGATTTAACCAGAGTTGACAACCTATGGTTAACCGAACTGGCTCCTCAGTTTTACGCTATGCAGAATTGA
Protein sequence

>MS.gene40397.t1

MVFGQRDRNKPFVARRNYQPNANANTNRNNQTHSSPRRQEIIEQRKSLPIASVEKRLIEEVRKNDILIIVGETGSGKTTQIPQFLFHAGFCHDGKVVGITQPRRVAAITVANRVAEECGCELGQKVGYSVRFDDSASNSTRIKYMTDGLLLREALLDPYLSKYSVIIVDEAHERTVHTDVLMGLLKNVQHARSNSIKDGRNLDNENKNTNGFMLDKDNGQNDTFLRKDHREKSSPLKLIIMSASLDARTFSEYFGGAKAVHVQGRQFPVDIFYTRHPETDYVDAALITIFQVHQDEAPGDILVFLTGQEEIDAVERLIKERLTKLPQENQKLQVVPIYAALPSEQQMRAFDPAPSGFRKVVLATNIAETSITIPGIKYVIDPGLVKARSYDPGKGMESLIVVPTSKSQALQRSGRAGREGPGKCFRLYPENEFEKLEDSTMPEIKRCNLSNVILQLKALGVDDILGFDFIEKPSRTAIIKSLEQLFLLGALTDDCQLSDPVGRQMARLPLDPVYAKALILASQFNCLEEMLIAVAMLSAESIFYFPREKYEEARTAAKSFASPEGDHITLINVYRAATDLLEKRTIETNKTKNEKEFRKWCKENFINSRSLRHARDIHRQIQGHVQQMGLKLASCGEDMLQFRRCLVASFFLNAAVKQPDGTYRALASGQVVQIHPSSVLFRKKPECIIFNELIQTNKKYVRDLTRVDNLWLTELAPQFYAMQN