AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40853

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40853.t1 MTR_5g045590 99.259 405 3 0 1 405 1 405 0.0 825
MS.gene40853.t1 MTR_2g105270 52.941 425 152 5 20 404 31 447 5.77e-143 414
MS.gene40853.t1 MTR_2g105270 53.714 350 114 5 20 329 31 372 2.58e-108 325
MS.gene40853.t1 MTR_2g105270 51.940 335 113 5 20 314 31 357 1.25e-97 296
MS.gene40853.t1 MTR_5g005240 40.092 434 207 8 19 403 14 443 4.95e-96 295
MS.gene40853.t1 MTR_8g088670 39.900 401 182 9 32 403 50 420 2.64e-83 261
MS.gene40853.t1 MTR_1g040495 39.370 381 181 7 40 400 15 365 1.60e-79 249
MS.gene40853.t1 MTR_8g094880 36.952 433 198 10 22 405 18 424 7.93e-75 239
MS.gene40853.t1 MTR_8g094905 36.952 433 198 10 22 405 18 424 7.93e-75 239
MS.gene40853.t1 MTR_1g076210 40.806 397 187 6 31 403 16 388 9.93e-75 238
MS.gene40853.t1 MTR_1g093280 40.806 397 187 6 31 403 16 388 9.93e-75 238
MS.gene40853.t1 MTR_1g106065 34.988 423 227 8 22 403 3 418 1.27e-74 238
MS.gene40853.t1 MTR_7g096170 38.619 391 194 6 32 400 17 383 8.48e-71 228
MS.gene40853.t1 MTR_7g096170 40.395 354 166 5 32 364 17 346 8.88e-66 214
MS.gene40853.t1 MTR_8g088670 40.323 310 130 7 32 314 50 331 8.92e-60 198
MS.gene40853.t1 MTR_8g088670 39.490 314 135 7 32 318 50 335 9.74e-60 197
MS.gene40853.t1 MTR_7g096170 40.252 318 145 5 32 328 17 310 6.73e-54 181
MS.gene40853.t1 MTR_7g096170 39.850 266 135 4 136 400 45 286 8.65e-45 157
MS.gene40853.t1 MTR_1g040475 40.796 201 103 3 27 211 4 204 1.20e-33 125
MS.gene40853.t1 MTR_1g076220 34.052 232 91 5 176 403 54 227 3.94e-30 116
MS.gene40853.t1 MTR_1g093370 42.105 133 69 3 275 403 37 165 4.69e-24 98.2
MS.gene40853.t1 MTR_2g059110 46.753 77 41 0 31 107 8 84 3.98e-17 76.6
MS.gene40853.t1 MTR_2g059120 53.968 63 29 0 45 107 20 82 2.25e-14 68.9
MS.gene40853.t1 MTR_2g100150 36.667 150 77 5 36 181 12 147 4.20e-14 70.1
MS.gene40853.t1 MTR_1g040455 36.264 91 57 1 314 403 7 97 9.86e-14 67.0
MS.gene40853.t1 MTR_1g055050 42.991 107 58 2 121 225 33 138 2.90e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40853.t1 AT2G03620 64.394 396 131 4 17 404 27 420 1.05e-164 469
MS.gene40853.t1 AT2G03620 64.394 396 131 4 17 404 27 420 1.05e-164 469
MS.gene40853.t1 AT1G16010 55.802 405 148 4 28 404 40 441 7.21e-148 427
MS.gene40853.t1 AT1G16010 55.802 405 148 4 28 404 40 441 7.21e-148 427
MS.gene40853.t1 AT1G16010 55.802 405 148 4 28 404 40 441 7.21e-148 427
MS.gene40853.t1 AT1G80900 52.955 423 149 4 20 404 32 442 8.98e-148 427
MS.gene40853.t1 AT1G80900 52.955 423 149 4 20 404 32 442 8.98e-148 427
MS.gene40853.t1 AT3G19640 41.096 438 184 11 40 403 46 483 2.46e-89 278
MS.gene40853.t1 AT5G09690 40.657 396 192 4 26 403 25 395 2.63e-89 276
MS.gene40853.t1 AT5G09690 40.657 396 192 4 26 403 14 384 2.88e-89 275
MS.gene40853.t1 AT5G09690 41.144 367 173 4 26 375 25 365 1.90e-83 260
MS.gene40853.t1 AT3G58970 37.750 400 200 7 32 403 56 434 3.90e-78 248
MS.gene40853.t1 AT5G09690 42.424 330 148 3 26 338 14 318 1.44e-72 230
MS.gene40853.t1 AT5G09690 42.424 330 148 3 26 338 25 329 1.78e-72 230
MS.gene40853.t1 AT5G09690 42.424 330 148 3 26 338 25 329 1.78e-72 230
MS.gene40853.t1 AT5G09690 42.424 330 148 3 26 338 14 318 2.48e-72 229
MS.gene40853.t1 AT5G09690 42.424 330 148 3 26 338 25 329 2.86e-72 230
MS.gene40853.t1 AT5G09690 42.424 330 148 3 26 338 14 318 2.96e-72 229
MS.gene40853.t1 AT5G64560 39.440 393 183 6 40 403 26 392 9.01e-68 220
MS.gene40853.t1 AT5G09690 36.111 396 195 6 26 403 14 369 1.97e-67 218
MS.gene40853.t1 AT5G64560 40.111 359 161 5 40 370 26 358 1.73e-61 203
MS.gene40853.t1 AT5G09690 46.047 215 95 2 26 223 14 224 3.32e-53 179
MS.gene40853.t1 AT5G09690 46.047 215 95 2 26 223 25 235 5.12e-53 179
MS.gene40853.t1 AT5G09690 46.047 215 95 2 26 223 25 235 5.12e-53 179
MS.gene40853.t1 AT5G09690 46.047 215 95 2 26 223 25 235 5.12e-53 179
MS.gene40853.t1 AT4G28580 31.579 399 214 6 29 403 44 407 2.83e-51 177
MS.gene40853.t1 AT5G09720 39.427 279 138 8 36 301 16 276 7.70e-47 162
MS.gene40853.t1 AT5G09720 39.427 279 138 8 36 301 16 276 7.70e-47 162

Find 91 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATCTTCCCTTTGAATTT+AGG 0.088653 5.2:+36203828 MS.gene40853:CDS
TGATCTTCCCTTTGAATTTA+GGG 0.214507 5.2:+36203829 MS.gene40853:CDS
GCCGCCGTGGCAGGAGTATT+TGG 0.234108 5.2:+36205388 MS.gene40853:CDS
TTCTGGATTCAATTTGGTTT+TGG 0.248178 5.2:+36205444 MS.gene40853:CDS
CTACTCTTCTGGATTCAATT+TGG 0.249380 5.2:+36205438 MS.gene40853:CDS
ATTTATCCTTCTTCCATATT+AGG 0.249449 5.2:+36203339 MS.gene40853:CDS
AGCCATCTCCCTTCCTAATA+TGG 0.295338 5.2:-36203352 None:intergenic
TTCTAGAGATCTTCGACTAT+TGG 0.307849 5.2:+36203305 MS.gene40853:CDS
TCCCTTTGAATTTAGGGCTC+TGG 0.324784 5.2:+36203835 MS.gene40853:CDS
GAACGTGTTCGAAGATTTAA+AGG 0.326254 5.2:+36204575 MS.gene40853:CDS
TTCCATGACCTCTCTGCTTC+AGG 0.330823 5.2:-36203194 None:intergenic
AATCGCAGGGCTGGTATAAA+AGG 0.334220 5.2:+36203171 MS.gene40853:CDS
GAAGCATCTCAAACTTTATC+AGG 0.362455 5.2:-36205333 None:intergenic
ATTGGAAATTCAAGTAAATA+TGG 0.367253 5.2:+36204938 MS.gene40853:CDS
GAAGAAGGATAAATGAATTT+CGG 0.372060 5.2:-36203330 None:intergenic
ATCTATCAGTACTCTCCTTC+TGG 0.372524 5.2:+36204553 MS.gene40853:CDS
AGTAAATATGGTAGTTCAAC+GGG 0.377045 5.2:+36204950 MS.gene40853:CDS
CCAAGGACTTTCAGCGGCAT+TGG 0.377986 5.2:+36204920 MS.gene40853:CDS
GCAATATTTGCCGCCGTGGC+AGG 0.398522 5.2:+36205379 MS.gene40853:CDS
ATGCACATCTTTAGATTCTC+AGG 0.428704 5.2:+36203877 MS.gene40853:CDS
ATATCCCGTGCTAGATGAAC+TGG 0.441183 5.2:+36204526 MS.gene40853:CDS
ATTTGAAAACAATCATTTGA+AGG 0.441921 5.2:-36204818 None:intergenic
GAGGTCATGGAAGTCGGTCT+TGG 0.450393 5.2:+36203205 MS.gene40853:CDS
TTGTCAACCTTGAACAAATC+CGG 0.460150 5.2:+36203379 MS.gene40853:CDS
GATTTGACAGGTAAATGAAC+TGG 0.464980 5.2:+36204493 MS.gene40853:intron
CCAATGCCGCTGAAAGTCCT+TGG 0.466774 5.2:-36204920 None:intergenic
TAGGGCTCTGGAGTTAGCTC+TGG 0.466890 5.2:+36203847 MS.gene40853:CDS
TTTCAAATTCGTTCACCATC+GGG 0.472477 5.2:+36204833 MS.gene40853:CDS
GTATCTAAAATAGAATAGTA+AGG 0.476408 5.2:-36205503 None:intergenic
TTTGACTCAGCGAGTTCAAA+AGG 0.483080 5.2:+36204610 MS.gene40853:CDS
TTAACAGGCAAGCGAATCGC+AGG 0.485254 5.2:+36203157 MS.gene40853:CDS
TCACCAGAGCGGTCACTTAG+TGG 0.486329 5.2:+36204884 MS.gene40853:CDS
CTCCAGAGCCCTAAATTCAA+AGG 0.486570 5.2:-36203837 None:intergenic
CACAGAAAGGATTCAGCCGC+TGG 0.488174 5.2:+36204985 MS.gene40853:CDS
GGCCTGAAGCAGAGAGGTCA+TGG 0.488760 5.2:+36203192 MS.gene40853:CDS
TCCAGAGCCCTAAATTCAAA+GGG 0.491102 5.2:-36203836 None:intergenic
TTATACACCGGATTTGTTCA+AGG 0.499716 5.2:-36203386 None:intergenic
TCCCTTCCTAATATGGAAGA+AGG 0.500264 5.2:-36203345 None:intergenic
AATAGTCGAAGATCTCTAGA+AGG 0.505041 5.2:-36203303 None:intergenic
TCAGCCGCTGGAAATGTTGC+TGG 0.507132 5.2:+36204997 MS.gene40853:CDS
AGTGACCGCTCTGGTGAAGT+AGG 0.509078 5.2:-36204878 None:intergenic
ACGGTACTGTTGGTCGGTAC+AGG 0.509486 5.2:+36203445 MS.gene40853:CDS
GTGACCGCTCTGGTGAAGTA+GGG 0.510030 5.2:-36204877 None:intergenic
ACAGGACAATATTATGCAAT+CGG 0.514813 5.2:+36203463 MS.gene40853:CDS
TCCTTCTTCCATATTAGGAA+GGG 0.517517 5.2:+36203344 MS.gene40853:CDS
ATCCTTCTTCCATATTAGGA+AGG 0.519036 5.2:+36203343 MS.gene40853:CDS
ACAGGTAAATGAACTGGAGA+TGG 0.521690 5.2:+36204499 MS.gene40853:intron
TGATGAAATAGAACATCTCA+TGG 0.526083 5.2:+36204733 MS.gene40853:CDS
AGGCAAGCGAATCGCAGGGC+TGG 0.527115 5.2:+36203162 MS.gene40853:CDS
CTTGTCAAGTGTCACAATCT+CGG 0.527722 5.2:-36203253 None:intergenic
AGATGTGTCTGACTGAGAAA+AGG 0.536746 5.2:+36204777 MS.gene40853:CDS
AAGTAAATATGGTAGTTCAA+CGG 0.547346 5.2:+36204949 MS.gene40853:CDS
TCTTACAGCAGCCACATTGG+TGG 0.559109 5.2:+36205354 MS.gene40853:CDS
AATTCACTCGACGGTACTGT+TGG 0.559813 5.2:+36203435 MS.gene40853:CDS
GGCCGCAATATTTGCCGCCG+TGG 0.566381 5.2:+36205375 MS.gene40853:CDS
AGCCCACTAAGTGACCGCTC+TGG 0.568756 5.2:-36204887 None:intergenic
CACCAGAGCGGTCACTTAGT+GGG 0.569271 5.2:+36204885 MS.gene40853:CDS
GCATCTCAAACTTTATCAGG+AGG 0.575972 5.2:-36205330 None:intergenic
TTCCATATTAGGAAGGGAGA+TGG 0.579126 5.2:+36203350 MS.gene40853:CDS
GTGGGCTTCAGATGTTGCCA+AGG 0.580454 5.2:+36204903 MS.gene40853:CDS
TGCCACGGCGGCAAATATTG+CGG 0.584897 5.2:-36205377 None:intergenic
CGAGATTGTGACACTTGACA+AGG 0.585644 5.2:+36203254 MS.gene40853:CDS
GCTTCTTACAGCAGCCACAT+TGG 0.587792 5.2:+36205351 MS.gene40853:CDS
TACTCTCAACACATTATCGT+CGG 0.589716 5.2:+36205045 MS.gene40853:CDS
TGAGGCCAGTTCATCTAGCA+CGG 0.589991 5.2:-36204531 None:intergenic
AGCAGAGAGGTCATGGAAGT+CGG 0.592681 5.2:+36203199 MS.gene40853:CDS
CAAAGAATACATCGACGACA+CGG 0.599106 5.2:+36205160 MS.gene40853:CDS
GAGGCCAGTTCATCTAGCAC+GGG 0.606072 5.2:-36204530 None:intergenic
CATGGATGACGATGGTGACA+TGG 0.607118 5.2:+36204751 MS.gene40853:CDS
ATCTTCGAACACGTTCCAGA+AGG 0.609844 5.2:-36204568 None:intergenic
ATTCATGAGGATGACTTCAT+CGG 0.612801 5.2:-36203415 None:intergenic
CATTCCAAATACTCCTGCCA+CGG 0.614342 5.2:-36205392 None:intergenic
TTTGAAATCACTCTACCCGA+TGG 0.614647 5.2:-36204848 None:intergenic
GAACTCGCTGAGTCAAAGCA+AGG 0.614782 5.2:-36204604 None:intergenic
TGACCGCTCTGGTGAAGTAG+GGG 0.617312 5.2:-36204876 None:intergenic
GTTCATCTGATAACACAGAA+AGG 0.620976 5.2:+36204972 MS.gene40853:CDS
TAACAGGCAAGCGAATCGCA+GGG 0.633785 5.2:+36203158 MS.gene40853:CDS
CACTCGACGGTACTGTTGGT+CGG 0.637311 5.2:+36203439 MS.gene40853:CDS
TGAGATGTTCTATTTCATCA+CGG 0.640698 5.2:-36204730 None:intergenic
AAGGAGAGTACTGATAGATG+AGG 0.645637 5.2:-36204549 None:intergenic
AAATATTGCGGCCACCAATG+TGG 0.649069 5.2:-36205365 None:intergenic
TACCGTCGAGTGAATTCATG+AGG 0.649158 5.2:-36203428 None:intergenic
TCCAAATACTCCTGCCACGG+CGG 0.649365 5.2:-36205389 None:intergenic
ATGTTGCCAAGGACTTTCAG+CGG 0.651077 5.2:+36204914 MS.gene40853:CDS
CATCGGCAGTGATTATACAC+CGG 0.664675 5.2:-36203398 None:intergenic
ATCCTCATGAATTCACTCGA+CGG 0.675706 5.2:+36203426 MS.gene40853:CDS
GCTTCCAGCAACATTTCCAG+CGG 0.677029 5.2:-36205001 None:intergenic
GAACATCTCATGGATGACGA+TGG 0.697862 5.2:+36204743 MS.gene40853:CDS
AATGCAATGGAAGAAACACA+AGG 0.739174 5.2:+36203099 None:intergenic
ATAAAAGGCCTGAAGCAGAG+AGG 0.740166 5.2:+36203186 MS.gene40853:CDS
CAGCCCCTACTTCACCAGAG+CGG 0.766346 5.2:+36204873 MS.gene40853:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAATATGTTATATTAATA+AGG + chr5.2:36204465-36204484 MS.gene40853:intron 10.0%
!! AAGAAAGAGAATTATAATTA+AGG - chr5.2:36204658-36204677 None:intergenic 15.0%
!! AGCATACATAATATTTATAT+CGG + chr5.2:36203543-36203562 MS.gene40853:intron 15.0%
!! CATTATGTTTACATTAAAAT+TGG + chr5.2:36203977-36203996 MS.gene40853:intron 15.0%
!!! AATTGTATATTTTCATGTTT+CGG + chr5.2:36204359-36204378 MS.gene40853:intron 15.0%
!!! AATTTTATTAAAAAGACATC+AGG - chr5.2:36204300-36204319 None:intergenic 15.0%
!! ATATGATATAAAGTGACAAA+AGG - chr5.2:36205237-36205256 None:intergenic 20.0%
!! ATTGGAAATTCAAGTAAATA+TGG + chr5.2:36204938-36204957 MS.gene40853:CDS 20.0%
!! ATTTGAAAACAATCATTTGA+AGG - chr5.2:36204821-36204840 None:intergenic 20.0%
!! CTAAATACATAATAAGTCAT+TGG + chr5.2:36204403-36204422 MS.gene40853:intron 20.0%
!! GTAACTTATATTTAGTTATC+AGG + chr5.2:36204706-36204725 MS.gene40853:intron 20.0%
!!! AGATTTTCTCAACATAAAAT+TGG + chr5.2:36205184-36205203 MS.gene40853:CDS 20.0%
!!! GTATCTAAAATAGAATAGTA+AGG - chr5.2:36205506-36205525 None:intergenic 20.0%
! AAATATAAGTTACTAGTTGC+AGG - chr5.2:36204699-36204718 None:intergenic 25.0%
! AAGTAAATATGGTAGTTCAA+CGG + chr5.2:36204949-36204968 MS.gene40853:CDS 25.0%
! ATTATATACTATGTAGACCA+CGG + chr5.2:36205263-36205282 MS.gene40853:intron 25.0%
! ATTGATGTCTTGTGAAAATA+TGG - chr5.2:36204441-36204460 None:intergenic 25.0%
! ATTTATCCTTCTTCCATATT+AGG + chr5.2:36203339-36203358 MS.gene40853:CDS 25.0%
! GAAATCAGTAAGCAATTATA+TGG - chr5.2:36203795-36203814 None:intergenic 25.0%
! GAAGAAGGATAAATGAATTT+CGG - chr5.2:36203333-36203352 None:intergenic 25.0%
! TGAAAAATTTACAAGATTGC+AGG - chr5.2:36204138-36204157 None:intergenic 25.0%
! TTCACTTTGATGAAAATAGA+AGG + chr5.2:36204154-36204173 MS.gene40853:intron 25.0%
!! CATTTTCATTAAACATCCTA+TGG + chr5.2:36204058-36204077 MS.gene40853:intron 25.0%
!! TGATATCAGTGTTTGAATTT+TGG + chr5.2:36204335-36204354 MS.gene40853:intron 25.0%
!! TTTTCAATCTATCTGTTACA+GGG + chr5.2:36205295-36205314 MS.gene40853:intron 25.0%
!!! TACATGAGTATGATTTTAAC+AGG + chr5.2:36203142-36203161 MS.gene40853:CDS 25.0%
AAAACAGTGTTTAGGCTTAA+CGG + chr5.2:36204108-36204127 MS.gene40853:intron 30.0%
ACAGATTCAATGCAAAAATG+TGG - chr5.2:36203941-36203960 None:intergenic 30.0%
ACAGGACAATATTATGCAAT+CGG + chr5.2:36203463-36203482 MS.gene40853:CDS 30.0%
AGGGAGAGAATATAATGTTT+AGG + chr5.2:36204221-36204240 MS.gene40853:intron 30.0%
AGTAAATATGGTAGTTCAAC+GGG + chr5.2:36204950-36204969 MS.gene40853:CDS 30.0%
ATGATCTTCCCTTTGAATTT+AGG + chr5.2:36203828-36203847 MS.gene40853:CDS 30.0%
ATTGAAAAGTTTATAGTCCG+TGG - chr5.2:36205283-36205302 None:intergenic 30.0%
GGATAAAAATTGATCAGGAT+GGG + chr5.2:36203226-36203245 MS.gene40853:CDS 30.0%
GTCTTGGATAAAAATTGATC+AGG + chr5.2:36203221-36203240 MS.gene40853:CDS 30.0%
TCAAACACTGATATCAAGTT+TGG - chr5.2:36204331-36204350 None:intergenic 30.0%
TCAATGAAGATAAGCAATGT+CGG - chr5.2:36205115-36205134 None:intergenic 30.0%
TGAGATGTTCTATTTCATCA+CGG - chr5.2:36204733-36204752 None:intergenic 30.0%
TGATCTTCCCTTTGAATTTA+GGG + chr5.2:36203829-36203848 MS.gene40853:CDS 30.0%
TGATGAAATAGAACATCTCA+TGG + chr5.2:36204733-36204752 MS.gene40853:CDS 30.0%
TGGATAAAAATTGATCAGGA+TGG + chr5.2:36203225-36203244 MS.gene40853:CDS 30.0%
! AATAAGGATCTTGATTTGAC+AGG + chr5.2:36204481-36204500 MS.gene40853:intron 30.0%
! ATAGTAGTGGAAGAATTATG+AGG + chr5.2:36203586-36203605 MS.gene40853:intron 30.0%
! CTTTTCAATCTATCTGTTAC+AGG + chr5.2:36205294-36205313 MS.gene40853:intron 30.0%
! TAGTAGTGGAAGAATTATGA+GGG + chr5.2:36203587-36203606 MS.gene40853:intron 30.0%
! TGAAATAGGATGTCTTTTGT+TGG + chr5.2:36203627-36203646 MS.gene40853:intron 30.0%
! TTCATTGATAATACTCTTGC+TGG + chr5.2:36205127-36205146 MS.gene40853:intron 30.0%
! TTTTCAAATTCGTTCACCAT+CGG + chr5.2:36204832-36204851 MS.gene40853:CDS 30.0%
!! TGACCAGAAACTTTACTTTT+TGG + chr5.2:36203907-36203926 MS.gene40853:intron 30.0%
!! TTCTAGTTTACAGCAAAAAG+AGG - chr5.2:36203520-36203539 None:intergenic 30.0%
!! TTCTGGATTCAATTTGGTTT+TGG + chr5.2:36205444-36205463 MS.gene40853:CDS 30.0%
!!! AATTTGGTTTTGGTAGTTAC+TGG + chr5.2:36205454-36205473 MS.gene40853:CDS 30.0%
!!! ACCAGAAACTTTACTTTTTG+GGG + chr5.2:36203909-36203928 MS.gene40853:intron 30.0%
!!! GACCAGAAACTTTACTTTTT+GGG + chr5.2:36203908-36203927 MS.gene40853:intron 30.0%
AATAGTCGAAGATCTCTAGA+AGG - chr5.2:36203306-36203325 None:intergenic 35.0%
AGAAGAGTAGTCAAAAACTG+AGG - chr5.2:36205428-36205447 None:intergenic 35.0%
ATCCTTCTTCCATATTAGGA+AGG + chr5.2:36203343-36203362 MS.gene40853:CDS 35.0%
ATGCACATCTTTAGATTCTC+AGG + chr5.2:36203877-36203896 MS.gene40853:CDS 35.0%
ATTCATGAGGATGACTTCAT+CGG - chr5.2:36203418-36203437 None:intergenic 35.0%
CAACTCACAAAACAGTGTTT+AGG + chr5.2:36204100-36204119 MS.gene40853:intron 35.0%
CAGAGAGAAAAATCTGGTAA+GGG + chr5.2:36203489-36203508 MS.gene40853:intron 35.0%
CTACTCTTCTGGATTCAATT+TGG + chr5.2:36205438-36205457 MS.gene40853:CDS 35.0%
GAAATTGCAGGAAAAACCAT+AGG - chr5.2:36204077-36204096 None:intergenic 35.0%
GAACGTGTTCGAAGATTTAA+AGG + chr5.2:36204575-36204594 MS.gene40853:CDS 35.0%
GATTCAATGCAAAAATGTGG+AGG - chr5.2:36203938-36203957 None:intergenic 35.0%
GATTTGACAGGTAAATGAAC+TGG + chr5.2:36204493-36204512 MS.gene40853:intron 35.0%
GCACTATTAGGAAATAGTAG+TGG + chr5.2:36203573-36203592 MS.gene40853:intron 35.0%
GTTCATCTGATAACACAGAA+AGG + chr5.2:36204972-36204991 MS.gene40853:CDS 35.0%
TACTCTCAACACATTATCGT+CGG + chr5.2:36205045-36205064 MS.gene40853:CDS 35.0%
TCCCCAAAAAGTAAAGTTTC+TGG - chr5.2:36203913-36203932 None:intergenic 35.0%
TCCTTCTTCCATATTAGGAA+GGG + chr5.2:36203344-36203363 MS.gene40853:CDS 35.0%
TGTAGCACTCAAGATATGTA+GGG + chr5.2:36204202-36204221 MS.gene40853:intron 35.0%
TTGTAGCACTCAAGATATGT+AGG + chr5.2:36204201-36204220 MS.gene40853:intron 35.0%
TTGTCAACCTTGAACAAATC+CGG + chr5.2:36203379-36203398 MS.gene40853:CDS 35.0%
TTTCAAATTCGTTCACCATC+GGG + chr5.2:36204833-36204852 MS.gene40853:CDS 35.0%
! GAAGCATCTCAAACTTTATC+AGG - chr5.2:36205336-36205355 None:intergenic 35.0%
! TTATACACCGGATTTGTTCA+AGG - chr5.2:36203389-36203408 None:intergenic 35.0%
! TTCTAGAGATCTTCGACTAT+TGG + chr5.2:36203305-36203324 MS.gene40853:CDS 35.0%
!! GTTTTGGTAGTTACTGGAAT+TGG + chr5.2:36205460-36205479 MS.gene40853:CDS 35.0%
!! TCAGTTTTTGACTACTCTTC+TGG + chr5.2:36205427-36205446 MS.gene40853:CDS 35.0%
ACAGGTAAATGAACTGGAGA+TGG + chr5.2:36204499-36204518 MS.gene40853:intron 40.0%
AGAAGGCTGCTAAAATCTCT+AGG + chr5.2:36204171-36204190 MS.gene40853:intron 40.0%
AGATGTGTCTGACTGAGAAA+AGG + chr5.2:36204777-36204796 MS.gene40853:CDS 40.0%
ATCCTCATGAATTCACTCGA+CGG + chr5.2:36203426-36203445 MS.gene40853:CDS 40.0%
ATCGGAGTTGTAGCACTATT+AGG + chr5.2:36203561-36203580 MS.gene40853:intron 40.0%
ATCTATCAGTACTCTCCTTC+TGG + chr5.2:36204553-36204572 MS.gene40853:CDS 40.0%
CAAAGAATACATCGACGACA+CGG + chr5.2:36205160-36205179 MS.gene40853:CDS 40.0%
CCAGAGAGAAAAATCTGGTA+AGG + chr5.2:36203488-36203507 MS.gene40853:intron 40.0%
CTTGTCAAGTGTCACAATCT+CGG - chr5.2:36203256-36203275 None:intergenic 40.0%
GCATCTCAAACTTTATCAGG+AGG - chr5.2:36205333-36205352 None:intergenic 40.0%
GTGGAAGAATTATGAGGGTA+CGG + chr5.2:36203592-36203611 MS.gene40853:intron 40.0%
TCCAGAGCCCTAAATTCAAA+GGG - chr5.2:36203839-36203858 None:intergenic 40.0%
TCCCTTCCTAATATGGAAGA+AGG - chr5.2:36203348-36203367 None:intergenic 40.0%
TTCCATATTAGGAAGGGAGA+TGG + chr5.2:36203350-36203369 MS.gene40853:CDS 40.0%
TTTGACTCAGCGAGTTCAAA+AGG + chr5.2:36204610-36204629 MS.gene40853:CDS 40.0%
! CCTTACCAGATTTTTCTCTC+TGG - chr5.2:36203491-36203510 None:intergenic 40.0%
! GTTCAAAAGGTTGATCAACG+TGG + chr5.2:36204623-36204642 MS.gene40853:intron 40.0%
! TGTGAGTTGTCTGAAATTGC+AGG - chr5.2:36204089-36204108 None:intergenic 40.0%
! TTTGAAATCACTCTACCCGA+TGG - chr5.2:36204851-36204870 None:intergenic 40.0%
!! AAGGAGAGTACTGATAGATG+AGG - chr5.2:36204552-36204571 None:intergenic 40.0%
AAATATTGCGGCCACCAATG+TGG - chr5.2:36205368-36205387 None:intergenic 45.0%
AATTCACTCGACGGTACTGT+TGG + chr5.2:36203435-36203454 MS.gene40853:CDS 45.0%
AGCCATCTCCCTTCCTAATA+TGG - chr5.2:36203355-36203374 None:intergenic 45.0%
ATAAAAGGCCTGAAGCAGAG+AGG + chr5.2:36203186-36203205 MS.gene40853:CDS 45.0%
ATATCCCGTGCTAGATGAAC+TGG + chr5.2:36204526-36204545 MS.gene40853:CDS 45.0%
ATCTTCGAACACGTTCCAGA+AGG - chr5.2:36204571-36204590 None:intergenic 45.0%
ATGTTGCCAAGGACTTTCAG+CGG + chr5.2:36204914-36204933 MS.gene40853:CDS 45.0%
CATCGGCAGTGATTATACAC+CGG - chr5.2:36203401-36203420 None:intergenic 45.0%
CATTCCAAATACTCCTGCCA+CGG - chr5.2:36205395-36205414 None:intergenic 45.0%
CGAGATTGTGACACTTGACA+AGG + chr5.2:36203254-36203273 MS.gene40853:CDS 45.0%
CTCCAGAGCCCTAAATTCAA+AGG - chr5.2:36203840-36203859 None:intergenic 45.0%
GAACATCTCATGGATGACGA+TGG + chr5.2:36204743-36204762 MS.gene40853:CDS 45.0%
TACCGTCGAGTGAATTCATG+AGG - chr5.2:36203431-36203450 None:intergenic 45.0%
! AATCGCAGGGCTGGTATAAA+AGG + chr5.2:36203171-36203190 MS.gene40853:CDS 45.0%
! GGCATTGAGTGGTGTGAAAT+AGG + chr5.2:36203613-36203632 MS.gene40853:intron 45.0%
! TCCCTTTGAATTTAGGGCTC+TGG + chr5.2:36203835-36203854 MS.gene40853:CDS 45.0%
AGCAGAGAGGTCATGGAAGT+CGG + chr5.2:36203199-36203218 MS.gene40853:CDS 50.0%
CATGGATGACGATGGTGACA+TGG + chr5.2:36204751-36204770 MS.gene40853:CDS 50.0%
CGGCTCCAGAGAGAAAAATC+TGG + chr5.2:36203483-36203502 MS.gene40853:CDS 50.0%
GAACTCGCTGAGTCAAAGCA+AGG - chr5.2:36204607-36204626 None:intergenic 50.0%
GCTTCCAGCAACATTTCCAG+CGG - chr5.2:36205004-36205023 None:intergenic 50.0%
GCTTCTTACAGCAGCCACAT+TGG + chr5.2:36205351-36205370 MS.gene40853:CDS 50.0%
TAACAGGCAAGCGAATCGCA+GGG + chr5.2:36203158-36203177 MS.gene40853:CDS 50.0%
TCTTACAGCAGCCACATTGG+TGG + chr5.2:36205354-36205373 MS.gene40853:CDS 50.0%
TGAGGCCAGTTCATCTAGCA+CGG - chr5.2:36204534-36204553 None:intergenic 50.0%
TTAACAGGCAAGCGAATCGC+AGG + chr5.2:36203157-36203176 MS.gene40853:CDS 50.0%
TTCCATGACCTCTCTGCTTC+AGG - chr5.2:36203197-36203216 None:intergenic 50.0%
! TATGAGGGTACGGCATTGAG+TGG + chr5.2:36203602-36203621 MS.gene40853:intron 50.0%
ACGGTACTGTTGGTCGGTAC+AGG + chr5.2:36203445-36203464 MS.gene40853:CDS 55.0%
AGTGACCGCTCTGGTGAAGT+AGG - chr5.2:36204881-36204900 None:intergenic 55.0%
CACAGAAAGGATTCAGCCGC+TGG + chr5.2:36204985-36205004 MS.gene40853:CDS 55.0%
CACCAGAGCGGTCACTTAGT+GGG + chr5.2:36204885-36204904 MS.gene40853:CDS 55.0%
CACTCGACGGTACTGTTGGT+CGG + chr5.2:36203439-36203458 MS.gene40853:CDS 55.0%
CCAAGGACTTTCAGCGGCAT+TGG + chr5.2:36204920-36204939 MS.gene40853:CDS 55.0%
CCAATGCCGCTGAAAGTCCT+TGG - chr5.2:36204923-36204942 None:intergenic 55.0%
GAGGCCAGTTCATCTAGCAC+GGG - chr5.2:36204533-36204552 None:intergenic 55.0%
GAGGTCATGGAAGTCGGTCT+TGG + chr5.2:36203205-36203224 MS.gene40853:CDS 55.0%
GTGACCGCTCTGGTGAAGTA+GGG - chr5.2:36204880-36204899 None:intergenic 55.0%
GTGGGCTTCAGATGTTGCCA+AGG + chr5.2:36204903-36204922 MS.gene40853:CDS 55.0%
TCACCAGAGCGGTCACTTAG+TGG + chr5.2:36204884-36204903 MS.gene40853:CDS 55.0%
TCAGCCGCTGGAAATGTTGC+TGG + chr5.2:36204997-36205016 MS.gene40853:CDS 55.0%
TCCAAATACTCCTGCCACGG+CGG - chr5.2:36205392-36205411 None:intergenic 55.0%
TGACCGCTCTGGTGAAGTAG+GGG - chr5.2:36204879-36204898 None:intergenic 55.0%
TGCCACGGCGGCAAATATTG+CGG - chr5.2:36205380-36205399 None:intergenic 55.0%
!! TAGGGCTCTGGAGTTAGCTC+TGG + chr5.2:36203847-36203866 MS.gene40853:CDS 55.0%
AGCCCACTAAGTGACCGCTC+TGG - chr5.2:36204890-36204909 None:intergenic 60.0%
CAGCCCCTACTTCACCAGAG+CGG + chr5.2:36204873-36204892 MS.gene40853:CDS 60.0%
GCAATATTTGCCGCCGTGGC+AGG + chr5.2:36205379-36205398 MS.gene40853:CDS 60.0%
GGCCTGAAGCAGAGAGGTCA+TGG + chr5.2:36203192-36203211 MS.gene40853:CDS 60.0%
AGGCAAGCGAATCGCAGGGC+TGG + chr5.2:36203162-36203181 MS.gene40853:CDS 65.0%
GGCCGCAATATTTGCCGCCG+TGG + chr5.2:36205375-36205394 MS.gene40853:CDS 65.0%
!! GCCGCCGTGGCAGGAGTATT+TGG + chr5.2:36205388-36205407 MS.gene40853:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 36203105 36205546 36203105 ID=MS.gene40853
chr5.2 mRNA 36203105 36205546 36203105 ID=MS.gene40853.t1;Parent=MS.gene40853
chr5.2 exon 36203105 36203504 36203105 ID=MS.gene40853.t1.exon1;Parent=MS.gene40853.t1
chr5.2 CDS 36203105 36203504 36203105 ID=cds.MS.gene40853.t1;Parent=MS.gene40853.t1
chr5.2 exon 36203828 36203898 36203828 ID=MS.gene40853.t1.exon2;Parent=MS.gene40853.t1
chr5.2 CDS 36203828 36203898 36203828 ID=cds.MS.gene40853.t1;Parent=MS.gene40853.t1
chr5.2 exon 36204503 36204631 36204503 ID=MS.gene40853.t1.exon3;Parent=MS.gene40853.t1
chr5.2 CDS 36204503 36204631 36204503 ID=cds.MS.gene40853.t1;Parent=MS.gene40853.t1
chr5.2 exon 36204728 36205066 36204728 ID=MS.gene40853.t1.exon4;Parent=MS.gene40853.t1
chr5.2 CDS 36204728 36205066 36204728 ID=cds.MS.gene40853.t1;Parent=MS.gene40853.t1
chr5.2 exon 36205158 36205205 36205158 ID=MS.gene40853.t1.exon5;Parent=MS.gene40853.t1
chr5.2 CDS 36205158 36205205 36205158 ID=cds.MS.gene40853.t1;Parent=MS.gene40853.t1
chr5.2 exon 36205316 36205546 36205316 ID=MS.gene40853.t1.exon6;Parent=MS.gene40853.t1
chr5.2 CDS 36205316 36205546 36205316 ID=cds.MS.gene40853.t1;Parent=MS.gene40853.t1
Gene Sequence

>MS.gene40853

ATGGAAGAAACACAAGGACAGTTTTATGTTTCTAGTCTACATGAGTATGATTTTAACAGGCAAGCGAATCGCAGGGCTGGTATAAAAGGCCTGAAGCAGAGAGGTCATGGAAGTCGGTCTTGGATAAAAATTGATCAGGATGGGAATTCCGAGATTGTGACACTTGACAAGGCTACGATTATGAGACATTGTTCTTTGCCTTCTAGAGATCTTCGACTATTGGATCCGAAATTCATTTATCCTTCTTCCATATTAGGAAGGGAGATGGCTATTGTTGTCAACCTTGAACAAATCCGGTGTATAATCACTGCCGATGAAGTCATCCTCATGAATTCACTCGACGGTACTGTTGGTCGGTACAGGACAATATTATGCAATCGGCTCCAGAGAGAAAAATCTGGTAAGGGTTGTTCCTCTTTTTGCTGTAAACTAGAATTTAGCATACATAATATTTATATCGGAGTTGTAGCACTATTAGGAAATAGTAGTGGAAGAATTATGAGGGTACGGCATTGAGTGGTGTGAAATAGGATGTCTTTTGTTGGAAACAATTTGAAAATCTTAATTGAATATGATACTTCTTCAGCTTTAAGTAGCTGTTGCGATTTGTTTCTTGAATGCTATATTTGTTTATTTTGCTTCAAACTATTGCGAGTGTTTTAAAGCTTTTCGATTGACGTAATTACTCCATATAATTGCTTACTGATTTCAATTTTTGAACAGATGATCTTCCCTTTGAATTTAGGGCTCTGGAGTTAGCTCTGGAATTGACATGCACATCTTTAGATTCTCAGGTATTAATTGACCAGAAACTTTACTTTTTGGGGAGTCCTCCACATTTTTGCATTGAATCTGTGATAGTTCATTTTCTACATTATGTTTACATTAAAATTGGCAGCACTGAGTTTATCTGCTTGCTTATTTTGATTAAATTATGTAGTTCATTATTAGCTCATTTTCATTAAACATCCTATGGTTTTTCCTGCAATTTCAGACAACTCACAAAACAGTGTTTAGGCTTAACGGACTGCCTGCAATCTTGTAAATTTTTCACTTTGATGAAAATAGAAGGCTGCTAAAATCTCTAGGATTAGTTTTGTAGCACTCAAGATATGTAGGGAGAGAATATAATGTTTAGGTAAAATCTGAAACTGTAAGATAAAATTCAAGATATTATTGTAGCATAACATAACCTGATGTCTTTTTAATAAAATTCAAGAGCTCCAAACTTGATATCAGTGTTTGAATTTTGGTAATTGTATATTTTCATGTTTCGGAGTTCTACATTATATAGTTTGCTAAATACATAATAAGTCATTGGTTAAGCTATTAGCCATATTTTCACAAGACATCAATCATCTCAAATATGTTATATTAATAAGGATCTTGATTTGACAGGTAAATGAACTGGAGATGGAAATATATCCCGTGCTAGATGAACTGGCCTCATCTATCAGTACTCTCCTTCTGGAACGTGTTCGAAGATTTAAAGGTCATCTCCTTGCTTTGACTCAGCGAGTTCAAAAGGTTGATCAACGTGGAATTCTCATCCTTAATTATAATTCTCTTTCTTTTTTCTAGCTTTCTGTGACCTGCAACTAGTAACTTATATTTAGTTATCAGGTCCGTGATGAAATAGAACATCTCATGGATGACGATGGTGACATGGCTGAGATGTGTCTGACTGAGAAAAGGAGAAGACTAGATGCTTCTCCTTCAAATGATTGTTTTCAAATTCGTTCACCATCGGGTAGAGTGATTTCAAAATCAGCCCCTACTTCACCAGAGCGGTCACTTAGTGGGCTTCAGATGTTGCCAAGGACTTTCAGCGGCATTGGAAATTCAAGTAAATATGGTAGTTCAACGGGTTCATCTGATAACACAGAAAGGATTCAGCCGCTGGAAATGTTGCTGGAAGCGTACTTTATCGTCATTGATAATACTCTCAACACATTATCGTCGGTATGTAAATGTTAACTTCATTCACAGCTGTAGTTTTAGTGTATACCGACATTGCTTATCTTCATTGATAATACTCTTGCTGGTAATACAGCTCAAAGAATACATCGACGACACGGAAGATTTTCTCAACATAAAATTGGTAAGCTAAGATTAACGATTAGCAACATCCTTTTGTCACTTTATATCATATATATTTATTATATACTATGTAGACCACGGACTATAAACTTTTCAATCTATCTGTTACAGGGAAATATTCAAAACCTCCTGATAAAGTTTGAGATGCTTCTTACAGCAGCCACATTGGTGGCCGCAATATTTGCCGCCGTGGCAGGAGTATTTGGAATGAACTTTGAAACCTCAGTTTTTGACTACTCTTCTGGATTCAATTTGGTTTTGGTAGTTACTGGAATTGGTTGTGTAGCATTGTATTTCGCCTTACTATTCTATTTTAGATACAAAAAAGTGCTTCCAGAATGA
Protein sequence

>MS.gene40853.t1

MEETQGQFYVSSLHEYDFNRQANRRAGIKGLKQRGHGSRSWIKIDQDGNSEIVTLDKATIMRHCSLPSRDLRLLDPKFIYPSSILGREMAIVVNLEQIRCIITADEVILMNSLDGTVGRYRTILCNRLQREKSDDLPFEFRALELALELTCTSLDSQVNELEMEIYPVLDELASSISTLLLERVRRFKGHLLALTQRVQKVRDEIEHLMDDDGDMAEMCLTEKRRRLDASPSNDCFQIRSPSGRVISKSAPTSPERSLSGLQMLPRTFSGIGNSSKYGSSTGSSDNTERIQPLEMLLEAYFIVIDNTLNTLSSLKEYIDDTEDFLNIKLGNIQNLLIKFEMLLTAATLVAAIFAAVAGVFGMNFETSVFDYSSGFNLVLVVTGIGCVALYFALLFYFRYKKVLPE