Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41741.t1 | XP_003624637.1 | 100 | 33 | 0 | 0 | 38 | 70 | 206 | 238 | 3.30E-07 | 63.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41741.t1 | Q2HVN6 | 100.0 | 33 | 0 | 0 | 38 | 70 | 206 | 238 | 2.4e-07 | 63.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene41741 | 0.871505 | 6.49E-67 | -1.69E-46 |
| MS.gene04917 | MS.gene41741 | 0.874036 | 9.24E-68 | -1.69E-46 |
| MS.gene050317 | MS.gene41741 | 0.813584 | 2.38E-51 | -1.69E-46 |
| MS.gene050752 | MS.gene41741 | 0.818409 | 1.99E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene41741 | 0.802766 | 4.80E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene41741 | 0.801007 | 1.10E-48 | -1.69E-46 |
| MS.gene051184 | MS.gene41741 | 0.813135 | 2.98E-51 | -1.69E-46 |
| MS.gene052296 | MS.gene41741 | 0.842568 | 2.39E-58 | -1.69E-46 |
| MS.gene052342 | MS.gene41741 | 0.806176 | 9.34E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene41741 | 0.829641 | 4.59E-55 | -1.69E-46 |
| MS.gene052818 | MS.gene41741 | -0.809893 | 1.51E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene41741 | 0.810801 | 9.62E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene41741 | 0.813257 | 2.81E-51 | -1.69E-46 |
| MS.gene056752 | MS.gene41741 | 0.866327 | 3.09E-65 | -1.69E-46 |
| MS.gene057999 | MS.gene41741 | 0.815772 | 7.79E-52 | -1.69E-46 |
| MS.gene058654 | MS.gene41741 | 0.834997 | 2.17E-56 | -1.69E-46 |
| MS.gene06011 | MS.gene41741 | 0.823642 | 1.24E-53 | -1.69E-46 |
| MS.gene060259 | MS.gene41741 | 0.818963 | 1.49E-52 | -1.69E-46 |
| MS.gene060702 | MS.gene41741 | 0.800085 | 1.70E-48 | -1.69E-46 |
| MS.gene060869 | MS.gene41741 | 0.80442 | 2.18E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41741.t1 | MTR_7g085850 | 100.000 | 47 | 0 | 0 | 24 | 70 | 192 | 238 | 2.56e-24 | 90.9 |
| MS.gene41741.t1 | MTR_6g028130 | 71.429 | 42 | 12 | 0 | 21 | 62 | 364 | 405 | 2.85e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTCTGTTTCAGCTTACT+AGG | 0.366926 | 7.2:+21967078 | None:intergenic |
| TTTCAGCTTACTAGGATCAT+TGG | 0.393959 | 7.2:+21967086 | None:intergenic |
| TGCAAAGTTAGAAGCACTCT+TGG | 0.412053 | 7.2:-21967043 | MS.gene41741:CDS |
| CAGATTAAAGAAGGTGAAGA+TGG | 0.424543 | 7.2:-21966997 | MS.gene41741:CDS |
| AGAAGCACTCTTGGAAAAGA+AGG | 0.514342 | 7.2:-21967034 | MS.gene41741:CDS |
| AAAAGAAGGCTGATCTAGAA+AGG | 0.535925 | 7.2:-21967020 | MS.gene41741:CDS |
| CTAGAAAGGCAGATTAAAGA+AGG | 0.541104 | 7.2:-21967006 | MS.gene41741:CDS |
| ATCCTCAATCTTGATTTGAG+TGG | 0.542150 | 7.2:+21967764 | None:intergenic |
| AAGATTGAGGATAAATCTGC+AGG | 0.542679 | 7.2:-21967753 | MS.gene41741:CDS |
| AGGTGGTGTAAAAGAAGATA+AGG | 0.558774 | 7.2:-21967733 | MS.gene41741:intron |
| CTCCACTCAAATCAAGATTG+AGG | 0.573595 | 7.2:-21967766 | MS.gene41741:CDS |
| AGGTCAAGATATTAACGTGA+AGG | 0.638283 | 7.2:-21966974 | MS.gene41741:CDS |
| ATTAAAGAAGGTGAAGATGG+AGG | 0.660144 | 7.2:-21966994 | MS.gene41741:CDS |
| ATTGAGGATAAATCTGCAGG+TGG | 0.690052 | 7.2:-21967750 | MS.gene41741:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTAAGAAAAAGAAGAATA+AGG | - | chr7.2:21967439-21967458 | MS.gene41741:intron | 20.0% |
| ! | AAAGAAGATGATAAGAAAGA+AGG | - | chr7.2:21967566-21967585 | MS.gene41741:intron | 25.0% |
| ! | ATAAGGATGAAGATAAAGAT+GGG | - | chr7.2:21967081-21967100 | MS.gene41741:CDS | 25.0% |
| ! | GAAAAAGAAGGATAAAAATG+AGG | - | chr7.2:21967472-21967491 | MS.gene41741:intron | 25.0% |
| ! | GAATAAGAAAAAGGAAAAGA+AGG | - | chr7.2:21967298-21967317 | MS.gene41741:intron | 25.0% |
| AAAAAGGAAAAGAAGGAGAA+GGG | - | chr7.2:21967305-21967324 | MS.gene41741:intron | 30.0% | |
| AAAGAAGGATAAAAATGAGG+AGG | - | chr7.2:21967475-21967494 | MS.gene41741:intron | 30.0% | |
| AGAGAAAGAGAAGAAACATA+AGG | - | chr7.2:21967064-21967083 | MS.gene41741:CDS | 30.0% | |
| CATAAGGATGAAGATAAAGA+TGG | - | chr7.2:21967080-21967099 | MS.gene41741:CDS | 30.0% | |
| GAAAAAGGAAAAGAAGGATA+AGG | - | chr7.2:21967106-21967125 | MS.gene41741:intron | 30.0% | |
| GAAGAAGAAAAAGGAAAAGA+AGG | - | chr7.2:21967205-21967224 | MS.gene41741:intron | 30.0% | |
| GGATAAGAAAGAGAAAAAGA+AGG | - | chr7.2:21967460-21967479 | MS.gene41741:intron | 30.0% | |
| GGATAAGGAGAATAAGAAAA+AGG | - | chr7.2:21967289-21967308 | MS.gene41741:intron | 30.0% | |
| TAAGGATGAAGATAAAGATG+GGG | - | chr7.2:21967082-21967101 | MS.gene41741:CDS | 30.0% | |
| !! | ATCTTCTTTTTCACATTTCG+CGG | + | chr7.2:21967555-21967574 | None:intergenic | 30.0% |
| AAAAGAAGGCTGATCTAGAA+AGG | - | chr7.2:21967720-21967739 | MS.gene41741:intron | 35.0% | |
| AAAGAAGATGGAGACAAAGA+TGG | - | chr7.2:21967329-21967348 | MS.gene41741:intron | 35.0% | |
| AAGATTGAGGATAAATCTGC+AGG | - | chr7.2:21966987-21967006 | MS.gene41741:CDS | 35.0% | |
| AGATAAAGATGGGGAGAAAA+AGG | - | chr7.2:21967091-21967110 | MS.gene41741:CDS | 35.0% | |
| AGGTCAAGATATTAACGTGA+AGG | - | chr7.2:21967766-21967785 | MS.gene41741:CDS | 35.0% | |
| AGGTGGTGTAAAAGAAGATA+AGG | - | chr7.2:21967007-21967026 | MS.gene41741:CDS | 35.0% | |
| ATTAAAGAAGGTGAAGATGG+AGG | - | chr7.2:21967746-21967765 | MS.gene41741:CDS | 35.0% | |
| CAGATTAAAGAAGGTGAAGA+TGG | - | chr7.2:21967743-21967762 | MS.gene41741:CDS | 35.0% | |
| CTAGAAAGGCAGATTAAAGA+AGG | - | chr7.2:21967734-21967753 | MS.gene41741:CDS | 35.0% | |
| GAAAAAGGAAAAGAAGGAGA+AGG | - | chr7.2:21967304-21967323 | MS.gene41741:intron | 35.0% | |
| GAAAGACAAGGAGAAGAAAA+AGG | - | chr7.2:21967370-21967389 | MS.gene41741:intron | 35.0% | |
| GAAGAAGAAGAAAGAAGACA+AGG | - | chr7.2:21967598-21967617 | MS.gene41741:intron | 35.0% | |
| GAAGAAGAAGAAATCTGGAA+AGG | - | chr7.2:21967625-21967644 | MS.gene41741:intron | 35.0% | |
| GAAGAAGTCTAAGAAAGACA+AGG | - | chr7.2:21967358-21967377 | MS.gene41741:intron | 35.0% | |
| GAAGAGAAGAAGAAGAAATC+TGG | - | chr7.2:21967620-21967639 | MS.gene41741:intron | 35.0% | |
| GACAAGAATGAAGATGATGA+GGG | - | chr7.2:21967401-21967420 | MS.gene41741:intron | 35.0% | |
| GGAGAAGAAAAAGGAAAAGA+AGG | - | chr7.2:21967379-21967398 | MS.gene41741:intron | 35.0% | |
| GGTTGAGATAGAAAAAGATC+TGG | - | chr7.2:21967028-21967047 | MS.gene41741:CDS | 35.0% | |
| GTTGATGTAGAGAATGTCAA+GGG | - | chr7.2:21967242-21967261 | MS.gene41741:intron | 35.0% | |
| TTTCAGCTTACTAGGATCAT+TGG | + | chr7.2:21967657-21967676 | None:intergenic | 35.0% | |
| ! | AGAAGATGGTGATGAAAAGA+AGG | - | chr7.2:21967268-21967287 | MS.gene41741:intron | 35.0% |
| ! | AGTTGATGTAGAGAATGTCA+AGG | - | chr7.2:21967241-21967260 | MS.gene41741:intron | 35.0% |
| ! | ATCCTCAATCTTGATTTGAG+TGG | + | chr7.2:21966979-21966998 | None:intergenic | 35.0% |
| ! | TGGTGATGAAAAGAAGGATA+AGG | - | chr7.2:21967274-21967293 | MS.gene41741:intron | 35.0% |
| AAATGAGGGCAAGGATAAGA+AGG | - | chr7.2:21967175-21967194 | MS.gene41741:intron | 40.0% | |
| AAGGAGAAGGGAAAAGAAGA+TGG | - | chr7.2:21967317-21967336 | MS.gene41741:intron | 40.0% | |
| AATGTCAAGGGTGAAGAAGA+TGG | - | chr7.2:21967254-21967273 | MS.gene41741:intron | 40.0% | |
| AGCTTCTGTTTCAGCTTACT+AGG | + | chr7.2:21967665-21967684 | None:intergenic | 40.0% | |
| ATTGAGGATAAATCTGCAGG+TGG | - | chr7.2:21966990-21967009 | MS.gene41741:CDS | 40.0% | |
| CAAAACTGATGTAGAGGAGA+AGG | - | chr7.2:21967151-21967170 | MS.gene41741:intron | 40.0% | |
| CTCCACTCAAATCAAGATTG+AGG | - | chr7.2:21966974-21966993 | MS.gene41741:CDS | 40.0% | |
| GAAGGACAAAACTGATGTAG+AGG | - | chr7.2:21967145-21967164 | MS.gene41741:intron | 40.0% | |
| GAAGGATAAAAATGAGGAGG+AGG | - | chr7.2:21967478-21967497 | MS.gene41741:intron | 40.0% | |
| GGACAAGAATGAAGATGATG+AGG | - | chr7.2:21967400-21967419 | MS.gene41741:intron | 40.0% | |
| GGACAAGGAGAAGAAGAAAA+AGG | - | chr7.2:21967196-21967215 | MS.gene41741:intron | 40.0% | |
| GGAGAAGAAGAACGAAAAGA+AGG | - | chr7.2:21967127-21967146 | MS.gene41741:intron | 40.0% | |
| GTAGAGGAGAAGGAAAATGA+GGG | - | chr7.2:21967161-21967180 | MS.gene41741:intron | 40.0% | |
| TGGGGAGAAAAAGGAAAAGA+AGG | - | chr7.2:21967100-21967119 | MS.gene41741:intron | 40.0% | |
| TGTAGAGGAGAAGGAAAATG+AGG | - | chr7.2:21967160-21967179 | MS.gene41741:intron | 40.0% | |
| ! | AAGAGGGCAAAGTATCTGTA+AGG | - | chr7.2:21967510-21967529 | MS.gene41741:intron | 40.0% |
| ! | AGAGGGCAAAGTATCTGTAA+GGG | - | chr7.2:21967511-21967530 | MS.gene41741:intron | 40.0% |
| !! | AGAAGCACTCTTGGAAAAGA+AGG | - | chr7.2:21967706-21967725 | MS.gene41741:intron | 40.0% |
| !! | TGCAAAGTTAGAAGCACTCT+TGG | - | chr7.2:21967697-21967716 | MS.gene41741:intron | 40.0% |
| GAGGAGGATGAGAAAGAAGA+GGG | - | chr7.2:21967494-21967513 | MS.gene41741:intron | 45.0% | |
| GAATGAAGATGATGAGGGCG+AGG | - | chr7.2:21967406-21967425 | MS.gene41741:intron | 50.0% | |
| GGAGAAGGAAAATGAGGGCA+AGG | - | chr7.2:21967166-21967185 | MS.gene41741:intron | 50.0% | |
| GGAGGAGGATGAGAAAGAAG+AGG | - | chr7.2:21967493-21967512 | MS.gene41741:intron | 50.0% | |
| GGGCAAGGATAAGAAGGACA+AGG | - | chr7.2:21967181-21967200 | MS.gene41741:intron | 50.0% | |
| GAAGATGATGAGGGCGAGGA+TGG | - | chr7.2:21967410-21967429 | MS.gene41741:intron | 55.0% | |
| GATGAGGGCGAGGATGGTGA+TGG | - | chr7.2:21967416-21967435 | MS.gene41741:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 21966966 | 21967796 | 21966966 | ID=MS.gene41741 |
| chr7.2 | mRNA | 21966966 | 21967796 | 21966966 | ID=MS.gene41741.t1;Parent=MS.gene41741 |
| chr7.2 | exon | 21967734 | 21967796 | 21967734 | ID=MS.gene41741.t1.exon1;Parent=MS.gene41741.t1 |
| chr7.2 | CDS | 21967734 | 21967796 | 21967734 | ID=cds.MS.gene41741.t1;Parent=MS.gene41741.t1 |
| chr7.2 | exon | 21966966 | 21967115 | 21966966 | ID=MS.gene41741.t1.exon2;Parent=MS.gene41741.t1 |
| chr7.2 | CDS | 21966966 | 21967115 | 21966966 | ID=cds.MS.gene41741.t1;Parent=MS.gene41741.t1 |
| Gene Sequence |
| Protein sequence |
