AlfalfaGEDB | MS.gene42330

M. sativa cultivar XinJiangDaYe / MS.gene42330

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42330.t1	XP_013462134.1	92.9	70	5	0	1	70	2	71	4.80E-25	124

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42330.t1	A0A072V2A3	92.9	70	5	0	1	70	2	71	3.4e-25	124.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049611	MS.gene42330	0.812849	3.45E-51	-1.69E-46
MS.gene050381	MS.gene42330	0.820951	5.23E-53	-1.69E-46
MS.gene050809	MS.gene42330	0.808027	3.79E-50	-1.69E-46
MS.gene05104	MS.gene42330	0.812671	3.77E-51	-1.69E-46
MS.gene051632	MS.gene42330	0.814976	1.17E-51	-1.69E-46
MS.gene05240	MS.gene42330	0.829746	4.33E-55	-1.69E-46
MS.gene053420	MS.gene42330	0.834984	2.18E-56	-1.69E-46
MS.gene054277	MS.gene42330	0.809205	2.12E-50	-1.69E-46
MS.gene054706	MS.gene42330	0.816512	5.32E-52	-1.69E-46
MS.gene054742	MS.gene42330	0.811322	7.42E-51	-1.69E-46
MS.gene055959	MS.gene42330	0.824071	9.84E-54	-1.69E-46
MS.gene056330	MS.gene42330	0.807809	4.22E-50	-1.69E-46
MS.gene056856	MS.gene42330	0.816829	4.52E-52	-1.69E-46
MS.gene056920	MS.gene42330	0.819496	1.13E-52	-1.69E-46
MS.gene057544	MS.gene42330	0.816672	4.90E-52	-1.69E-46
MS.gene058068	MS.gene42330	0.812367	4.39E-51	-1.69E-46
MS.gene058093	MS.gene42330	0.812788	3.55E-51	-1.69E-46
MS.gene058099	MS.gene42330	0.842927	1.92E-58	-1.69E-46
MS.gene058269	MS.gene42330	0.80007	1.71E-48	-1.69E-46
MS.gene060405	MS.gene42330	0.800774	1.23E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42330.t1	MTR_3g112000	88.525	122	12	2	1	121	2	122	5.85e-70	206

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 28 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAATTCCAACATTTGTGTTC+TGG	0.239933	3.2:-89361803	MS.gene42330:CDS
AATTCCAACATTTGTGTTCT+GGG	0.279236	3.2:-89361802	MS.gene42330:CDS
GATACGGTTGCAGCTGGTGC+TGG	0.372363	3.2:+89361477	None:intergenic
TTTGACTTGGACTTGTACTT+TGG	0.409420	3.2:+89361368	None:intergenic
CTCATGTATCTTGTTTGACT+TGG	0.410489	3.2:+89361355	None:intergenic
ATACGGTTGCAGCTGGTGCT+GGG	0.483882	3.2:+89361478	None:intergenic
CAAGTCAGTGGTTGTAGAGC+AGG	0.484666	3.2:-89361508	MS.gene42330:CDS
CTGCTCTACAACCACTGACT+TGG	0.511839	3.2:+89361509	None:intergenic
ACCACTGACTTGGCAGGTGG+CGG	0.515445	3.2:+89361519	None:intergenic
TTTACTATTTGATGCAAGGT+CGG	0.516012	3.2:+89361725	None:intergenic
TTGTACTTTGGAAGCATCAT+CGG	0.516399	3.2:+89361380	None:intergenic
TCTACAACCACTGACTTGGC+AGG	0.516779	3.2:+89361513	None:intergenic
ATTCTTTACTATTTGATGCA+AGG	0.520583	3.2:+89361721	None:intergenic
GTGTTGGTGTGATTATTGTA+AGG	0.522525	3.2:+89361775	None:intergenic
AAAGCCCAGAACACAAATGT+TGG	0.544379	3.2:+89361798	None:intergenic
ATGAAGGTAAGAAGAATGAT+CGG	0.544526	3.2:-89361573	MS.gene42330:CDS
TGAAGGTAAGAAGAATGATC+GGG	0.567599	3.2:-89361572	MS.gene42330:CDS
TGGCAATGGTAATAATGATA+CGG	0.579053	3.2:+89361461	None:intergenic
AATAATGATACGGTTGCAGC+TGG	0.580071	3.2:+89361471	None:intergenic
ACCGCCACCTGCCAAGTCAG+TGG	0.586261	3.2:-89361520	MS.gene42330:CDS
TGATTATGATGCGATTGTGA+TGG	0.609805	3.2:+89361435	None:intergenic
AAATATAACAGAGACAATGA+AGG	0.611259	3.2:-89361589	MS.gene42330:CDS
TGATGCGATTGTGATGGAGT+TGG	0.619596	3.2:+89361441	None:intergenic
TACTTTGGAAGCATCATCGG+TGG	0.630715	3.2:+89361383	None:intergenic
ACAACCACTGACTTGGCAGG+TGG	0.640558	3.2:+89361516	None:intergenic
TCGGACAAGAGAATTAGTGT+TGG	0.641471	3.2:+89361759	None:intergenic
TACGGTTGCAGCTGGTGCTG+GGG	0.642249	3.2:+89361479	None:intergenic
GATTGTGATGGAGTTGGCAA+TGG	0.656251	3.2:+89361447	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAATTAATTGTATCAATTT+TGG	+	chr3.2:89361519-89361538	None:intergenic	10.0%
!!!	AATTAATTTTCTCTTTAATT+AGG	-	chr3.2:89361529-89361548	MS.gene42330:CDS	10.0%
!	AAATATAACAGAGACAATGA+AGG	-	chr3.2:89361589-89361608	MS.gene42330:CDS	25.0%
!	ATTCTTTACTATTTGATGCA+AGG	+	chr3.2:89361460-89361479	None:intergenic	25.0%
	AATTCCAACATTTGTGTTCT+GGG	-	chr3.2:89361376-89361395	MS.gene42330:CDS	30.0%
	ATGAAGGTAAGAAGAATGAT+CGG	-	chr3.2:89361605-89361624	MS.gene42330:intron	30.0%
	TAATTCCAACATTTGTGTTC+TGG	-	chr3.2:89361375-89361394	MS.gene42330:CDS	30.0%
	TGGCAATGGTAATAATGATA+CGG	+	chr3.2:89361720-89361739	None:intergenic	30.0%
	TGTTATATTTCCTGCAAAGT+AGG	+	chr3.2:89361579-89361598	None:intergenic	30.0%
	TTTACTATTTGATGCAAGGT+CGG	+	chr3.2:89361456-89361475	None:intergenic	30.0%
!!	TGTATCAATTTTGGTGAACA+AGG	+	chr3.2:89361510-89361529	None:intergenic	30.0%
	AGTAGGACACAGAGATTAAT+TGG	+	chr3.2:89361562-89361581	None:intergenic	35.0%
	TGAAGGTAAGAAGAATGATC+GGG	-	chr3.2:89361606-89361625	MS.gene42330:intron	35.0%
	TGATTATGATGCGATTGTGA+TGG	+	chr3.2:89361746-89361765	None:intergenic	35.0%
!	GTGTTGGTGTGATTATTGTA+AGG	+	chr3.2:89361406-89361425	None:intergenic	35.0%
!!	TTGTACTTTGGAAGCATCAT+CGG	+	chr3.2:89361801-89361820	None:intergenic	35.0%
!!	TTTGACTTGGACTTGTACTT+TGG	+	chr3.2:89361813-89361832	None:intergenic	35.0%
	AAAGCCCAGAACACAAATGT+TGG	+	chr3.2:89361383-89361402	None:intergenic	40.0%
	AATAATGATACGGTTGCAGC+TGG	+	chr3.2:89361710-89361729	None:intergenic	40.0%
	TCGGACAAGAGAATTAGTGT+TGG	+	chr3.2:89361422-89361441	None:intergenic	40.0%
!!	AAGGTCGGCTTTTGAATTCT+CGG	+	chr3.2:89361441-89361460	None:intergenic	40.0%
	ATCTCTGTGTCCTACTTTGC+AGG	-	chr3.2:89361566-89361585	MS.gene42330:CDS	45.0%
!	GATTGTGATGGAGTTGGCAA+TGG	+	chr3.2:89361734-89361753	None:intergenic	45.0%
!	TGATGCGATTGTGATGGAGT+TGG	+	chr3.2:89361740-89361759	None:intergenic	45.0%
!!	TACTTTGGAAGCATCATCGG+TGG	+	chr3.2:89361798-89361817	None:intergenic	45.0%
	CAAGTCAGTGGTTGTAGAGC+AGG	-	chr3.2:89361670-89361689	MS.gene42330:intron	50.0%
!	CTGCTCTACAACCACTGACT+TGG	+	chr3.2:89361672-89361691	None:intergenic	50.0%
!!	TCTACAACCACTGACTTGGC+AGG	+	chr3.2:89361668-89361687	None:intergenic	50.0%
	ATACGGTTGCAGCTGGTGCT+GGG	+	chr3.2:89361703-89361722	None:intergenic	55.0%
!!	ACAACCACTGACTTGGCAGG+TGG	+	chr3.2:89361665-89361684	None:intergenic	55.0%
	GATACGGTTGCAGCTGGTGC+TGG	+	chr3.2:89361704-89361723	None:intergenic	60.0%
!	TACGGTTGCAGCTGGTGCTG+GGG	+	chr3.2:89361702-89361721	None:intergenic	60.0%
!!	ACCACTGACTTGGCAGGTGG+CGG	+	chr3.2:89361662-89361681	None:intergenic	60.0%
	ACCGCCACCTGCCAAGTCAG+TGG	-	chr3.2:89361658-89361677	MS.gene42330:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	89361356	89361844	89361356	ID=MS.gene42330
chr3.2	mRNA	89361356	89361844	89361356	ID=MS.gene42330.t1;Parent=MS.gene42330
chr3.2	exon	89361730	89361844	89361730	ID=MS.gene42330.t1.exon1;Parent=MS.gene42330.t1
chr3.2	CDS	89361730	89361844	89361730	ID=cds.MS.gene42330.t1;Parent=MS.gene42330.t1
chr3.2	exon	89361356	89361612	89361356	ID=MS.gene42330.t1.exon2;Parent=MS.gene42330.t1
chr3.2	CDS	89361356	89361612	89361356	ID=cds.MS.gene42330.t1;Parent=MS.gene42330.t1

Gene Sequence

>MS.gene42330

ATGATGAGAGAAAGAACTCTAATTCCAACATTTGTGTTCTGGGCTTTCCTTACAATAATCACACCAACACTAATTCTCTTGTCCGAGAATTCAAAAGCCGACCTTGCATCAAATAGTAAAGAATCTTGTAAACTACTAAGTTACTAACACTCCTTGTTCACCAAAATTGATACAATTAATTTTCTCTTTAATTAGGCTTGTGTCCAATTAATCTCTGTGTCCTACTTTGCAGGAAATATAACAGAGACAATGAAGGTAAGAAGAATGATCGGGCACACACAGAGTAACATCATAAGAACAGCACCGCCACCTGCCAAGTCAGTGGTTGTAGAGCAGGAACTTGCCCCAGCACCAGCTGCAACCGTATCATTATTACCATTGCCAACTCCATCACAATCGCATCATAATCACACTGATCTTATAAGCAGCAATCACAATACCACCGATGATGCTTCCAAAGTACAAGTCCAAGTCAAACAAGATACATGA

Protein sequence

>MS.gene42330.t1

MMRERTLIPTFVFWAFLTIITPTLILLSENSKADLASNRNITETMKVRRMIGHTQSNIIRTAPPPAKSVVVEQELAPAPAATVSLLPLPTPSQSHHNHTDLISSNHNTTDDASKVQVQVKQDT