Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43213.t1 | XP_013457864.1 | 97.5 | 1238 | 31 | 0 | 1 | 1238 | 1 | 1238 | 0.00E+00 | 2416.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43213.t1 | F4IDY5 | 48.4 | 1259 | 591 | 14 | 1 | 1238 | 1 | 1221 | 0.0e+00 | 1076.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43213.t1 | A0A072V1D4 | 97.5 | 1238 | 31 | 0 | 1 | 1238 | 1 | 1238 | 0.0e+00 | 2416.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050809 | MS.gene43213 | 0.821189 | 4.61E-53 | -1.69E-46 |
| MS.gene05240 | MS.gene43213 | 0.800161 | 1.64E-48 | -1.69E-46 |
| MS.gene052702 | MS.gene43213 | 0.824235 | 9.01E-54 | -1.69E-46 |
| MS.gene054181 | MS.gene43213 | 0.818285 | 2.12E-52 | -1.69E-46 |
| MS.gene054290 | MS.gene43213 | 0.839328 | 1.69E-57 | -1.69E-46 |
| MS.gene054742 | MS.gene43213 | 0.820075 | 8.31E-53 | -1.69E-46 |
| MS.gene055158 | MS.gene43213 | 0.801093 | 1.06E-48 | -1.69E-46 |
| MS.gene055601 | MS.gene43213 | 0.812606 | 3.90E-51 | -1.69E-46 |
| MS.gene056590 | MS.gene43213 | 0.818057 | 2.39E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene43213 | 0.815434 | 9.26E-52 | -1.69E-46 |
| MS.gene056697 | MS.gene43213 | 0.800873 | 1.18E-48 | -1.69E-46 |
| MS.gene056856 | MS.gene43213 | 0.830042 | 3.67E-55 | -1.69E-46 |
| MS.gene056920 | MS.gene43213 | 0.802921 | 4.46E-49 | -1.69E-46 |
| MS.gene057544 | MS.gene43213 | 0.815045 | 1.13E-51 | -1.69E-46 |
| MS.gene058158 | MS.gene43213 | 0.834516 | 2.86E-56 | -1.69E-46 |
| MS.gene059402 | MS.gene43213 | 0.81582 | 7.60E-52 | -1.69E-46 |
| MS.gene060405 | MS.gene43213 | 0.803704 | 3.07E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43213.t1 | MTR_4g107440 | 97.417 | 1239 | 32 | 0 | 1 | 1239 | 1 | 1239 | 0.0 | 2506 |
| MS.gene43213.t1 | MTR_6g083900 | 33.834 | 866 | 480 | 16 | 29 | 833 | 36 | 869 | 1.08e-129 | 430 |
| MS.gene43213.t1 | MTR_6g083900 | 53.497 | 286 | 131 | 2 | 954 | 1238 | 907 | 1191 | 2.01e-101 | 351 |
| MS.gene43213.t1 | MTR_6g083900 | 20.067 | 598 | 375 | 19 | 257 | 846 | 8 | 510 | 2.27e-16 | 85.5 |
| MS.gene43213.t1 | MTR_6g083900 | 25.862 | 232 | 150 | 5 | 620 | 849 | 45 | 256 | 2.95e-16 | 85.1 |
| MS.gene43213.t1 | MTR_6g083900 | 26.144 | 306 | 171 | 8 | 38 | 300 | 642 | 935 | 1.53e-12 | 73.2 |
| MS.gene43213.t1 | MTR_8g104410 | 56.643 | 286 | 122 | 2 | 954 | 1238 | 681 | 965 | 2.69e-104 | 355 |
| MS.gene43213.t1 | MTR_8g104410 | 43.983 | 241 | 121 | 3 | 609 | 835 | 405 | 645 | 4.43e-52 | 200 |
| MS.gene43213.t1 | MTR_8g104410 | 30.622 | 209 | 136 | 3 | 301 | 506 | 66 | 268 | 3.59e-17 | 88.2 |
| MS.gene43213.t1 | MTR_8g104410 | 23.970 | 267 | 171 | 8 | 603 | 859 | 52 | 296 | 1.36e-13 | 76.3 |
| MS.gene43213.t1 | MTR_8g104410 | 24.762 | 210 | 144 | 5 | 38 | 243 | 69 | 268 | 7.96e-13 | 73.9 |
| MS.gene43213.t1 | MTR_8g104410 | 27.358 | 212 | 118 | 6 | 38 | 219 | 416 | 621 | 4.69e-12 | 71.2 |
| MS.gene43213.t1 | MTR_8g104410 | 56.643 | 286 | 122 | 2 | 954 | 1238 | 917 | 1201 | 1.14e-103 | 358 |
| MS.gene43213.t1 | MTR_8g104410 | 32.882 | 517 | 315 | 8 | 2 | 506 | 8 | 504 | 4.02e-67 | 249 |
| MS.gene43213.t1 | MTR_8g104410 | 43.983 | 241 | 121 | 3 | 609 | 835 | 641 | 881 | 1.28e-51 | 199 |
| MS.gene43213.t1 | MTR_8g104410 | 27.727 | 220 | 137 | 6 | 620 | 837 | 40 | 239 | 1.28e-17 | 89.7 |
| MS.gene43213.t1 | MTR_8g104410 | 23.970 | 267 | 171 | 8 | 603 | 859 | 288 | 532 | 2.68e-13 | 75.5 |
| MS.gene43213.t1 | MTR_8g104410 | 27.358 | 212 | 118 | 6 | 38 | 219 | 652 | 857 | 5.99e-12 | 71.2 |
| MS.gene43213.t1 | MTR_2g020040 | 38.140 | 215 | 116 | 6 | 1034 | 1236 | 16 | 225 | 2.86e-42 | 156 |
| MS.gene43213.t1 | MTR_4g057310 | 37.069 | 232 | 126 | 8 | 1019 | 1236 | 1 | 226 | 5.06e-42 | 156 |
| MS.gene43213.t1 | MTR_3g108240 | 35.200 | 250 | 134 | 8 | 1011 | 1237 | 29 | 273 | 2.55e-41 | 155 |
| MS.gene43213.t1 | MTR_3g108240 | 34.340 | 265 | 151 | 9 | 991 | 1237 | 12 | 271 | 2.69e-40 | 152 |
| MS.gene43213.t1 | MTR_1g047740 | 34.426 | 244 | 135 | 9 | 1013 | 1236 | 26 | 264 | 6.30e-40 | 150 |
| MS.gene43213.t1 | MTR_4g057310 | 37.220 | 223 | 120 | 8 | 1017 | 1225 | 2 | 218 | 8.27e-40 | 147 |
| MS.gene43213.t1 | MTR_7g102990 | 35.484 | 217 | 120 | 8 | 1034 | 1236 | 22 | 232 | 1.97e-37 | 143 |
| MS.gene43213.t1 | MTR_7g102990 | 37.143 | 175 | 91 | 7 | 1034 | 1195 | 22 | 190 | 2.79e-31 | 123 |
| MS.gene43213.t1 | MTR_1g010150 | 34.706 | 170 | 102 | 6 | 1068 | 1233 | 1 | 165 | 1.60e-24 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43213.t1 | AT1G74770 | 48.810 | 1260 | 591 | 20 | 1 | 1239 | 1 | 1227 | 0.0 | 1125 |
| MS.gene43213.t1 | AT1G18910 | 48.377 | 1263 | 587 | 17 | 1 | 1239 | 1 | 1222 | 0.0 | 1114 |
| MS.gene43213.t1 | AT1G74770 | 48.348 | 1241 | 587 | 20 | 1 | 1220 | 1 | 1208 | 0.0 | 1093 |
| MS.gene43213.t1 | AT3G18290 | 31.828 | 886 | 526 | 16 | 37 | 863 | 53 | 919 | 2.58e-122 | 410 |
| MS.gene43213.t1 | AT3G18290 | 54.452 | 292 | 128 | 1 | 952 | 1238 | 917 | 1208 | 8.85e-106 | 364 |
| MS.gene43213.t1 | AT3G18290 | 25.203 | 246 | 159 | 6 | 613 | 853 | 44 | 269 | 7.77e-15 | 80.5 |
| MS.gene43213.t1 | AT3G62970 | 37.083 | 240 | 127 | 8 | 1007 | 1233 | 11 | 239 | 3.92e-42 | 155 |
| MS.gene43213.t1 | AT3G62970 | 37.083 | 240 | 127 | 8 | 1007 | 1233 | 11 | 239 | 2.33e-41 | 154 |
| MS.gene43213.t1 | AT3G62970 | 38.158 | 228 | 117 | 8 | 1007 | 1221 | 11 | 227 | 3.75e-41 | 152 |
| MS.gene43213.t1 | AT5G22920 | 39.234 | 209 | 109 | 6 | 1034 | 1229 | 25 | 228 | 1.25e-40 | 150 |
| MS.gene43213.t1 | AT5G22920 | 39.234 | 209 | 109 | 6 | 1034 | 1229 | 25 | 228 | 3.78e-40 | 151 |
| MS.gene43213.t1 | AT5G25560 | 36.323 | 223 | 122 | 8 | 1034 | 1243 | 61 | 276 | 7.23e-38 | 145 |
| MS.gene43213.t1 | AT5G25560 | 36.323 | 223 | 122 | 8 | 1034 | 1243 | 61 | 276 | 7.23e-38 | 145 |
| MS.gene43213.t1 | AT5G25560 | 36.323 | 223 | 122 | 8 | 1034 | 1243 | 61 | 276 | 7.23e-38 | 145 |
| MS.gene43213.t1 | AT5G25560 | 36.323 | 223 | 122 | 8 | 1034 | 1243 | 61 | 276 | 7.23e-38 | 145 |
| MS.gene43213.t1 | AT5G18650 | 35.849 | 212 | 119 | 6 | 1034 | 1233 | 16 | 222 | 4.85e-37 | 141 |
| MS.gene43213.t1 | AT5G25560 | 34.764 | 233 | 122 | 9 | 1034 | 1243 | 61 | 286 | 5.15e-36 | 140 |
| MS.gene43213.t1 | AT5G25560 | 35.321 | 218 | 121 | 8 | 1039 | 1243 | 61 | 271 | 2.94e-34 | 134 |
Find 232 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTCTTCTGACAAATATT+TGG | 0.159497 | 8.4:+19453033 | None:intergenic |
| TTCTTTCAAATCTTTCTTAA+TGG | 0.165016 | 8.4:+19455289 | None:intergenic |
| CTCATACTTGCTCACACTTT+TGG | 0.185809 | 8.4:+19453906 | None:intergenic |
| AACCACAACAAGGGCATTTA+TGG | 0.207892 | 8.4:+19447941 | None:intergenic |
| TTGTCCTATTCCTCATCTTC+TGG | 0.209206 | 8.4:-19453931 | MS.gene43213:CDS |
| AATATCAGGTCAATTGGTTT+TGG | 0.241534 | 8.4:+19453766 | None:intergenic |
| GAGAAGAATTCTCTAATTAT+AGG | 0.251609 | 8.4:+19453316 | None:intergenic |
| ACTTATCCAGCTAAAATTCT+TGG | 0.265565 | 8.4:-19455201 | MS.gene43213:CDS |
| CTAATAAGAAATTCCGGTTC+TGG | 0.275911 | 8.4:-19455248 | MS.gene43213:CDS |
| ACTTCCTCCAGCAACATTAT+AGG | 0.279336 | 8.4:+19455741 | None:intergenic |
| CAAGGCTATTAAGAAAGATT+TGG | 0.279486 | 8.4:-19453734 | MS.gene43213:CDS |
| TGGTTCCGCATCGGTTATTC+AGG | 0.281471 | 8.4:-19454102 | MS.gene43213:CDS |
| TCATATAACTCTAGTGTTAT+AGG | 0.281765 | 8.4:+19447918 | None:intergenic |
| AGATTAAAGTTCTCCATCTT+TGG | 0.282699 | 8.4:-19455319 | MS.gene43213:CDS |
| CTGATGTCCTCATCATTTAC+AGG | 0.283854 | 8.4:-19455178 | MS.gene43213:intron |
| CCAAGTGACTTGCTACATAT+TGG | 0.304623 | 8.4:+19449740 | None:intergenic |
| AATCAGTAGTAGAAACTAAA+AGG | 0.319009 | 8.4:+19453461 | None:intergenic |
| GTGCATCTAACATCCTAAAA+AGG | 0.326071 | 8.4:+19448165 | None:intergenic |
| CTGCAGTTCTTTCTAAAGAT+AGG | 0.327534 | 8.4:+19454306 | None:intergenic |
| GATTTGGATTACCTAGTTCT+TGG | 0.327987 | 8.4:-19453718 | MS.gene43213:CDS |
| CTTGAACATAACAGCACTAA+TGG | 0.329040 | 8.4:-19457149 | MS.gene43213:CDS |
| ACCGCGAGGAAATAGAAATT+TGG | 0.329639 | 8.4:-19453339 | MS.gene43213:CDS |
| CAAGAGGAACTGTAAGCTTT+TGG | 0.340400 | 8.4:-19451472 | MS.gene43213:CDS |
| AGATCCTTCCTTATCGGATA+TGG | 0.341556 | 8.4:-19459289 | MS.gene43213:CDS |
| ATGCCGAGTTGGAAAGGGTT+TGG | 0.341833 | 8.4:-19450049 | MS.gene43213:CDS |
| TGCCGAGTTGGAAAGGGTTT+GGG | 0.344960 | 8.4:-19450048 | MS.gene43213:CDS |
| TGCTGAAACTGCGTCGTCCT+TGG | 0.348291 | 8.4:-19459124 | MS.gene43213:CDS |
| GATCCTCATGAACAAATATA+TGG | 0.349680 | 8.4:-19451507 | MS.gene43213:CDS |
| GGATCCAGAAGATGAGGAAT+AGG | 0.349817 | 8.4:+19453927 | None:intergenic |
| TTGGTCCGAGTTCATTCAAT+AGG | 0.354396 | 8.4:-19455409 | MS.gene43213:CDS |
| GGAAATAGTGTTCCCAGCTT+TGG | 0.358463 | 8.4:-19453596 | MS.gene43213:CDS |
| AGCATGAAGTTGGTTGCAAC+AGG | 0.361074 | 8.4:+19451446 | None:intergenic |
| TAGTCAGAAGATCTCTTCTA+TGG | 0.362823 | 8.4:-19451586 | MS.gene43213:CDS |
| CCTCCGCATCACTATGGATT+TGG | 0.369354 | 8.4:+19453620 | None:intergenic |
| GTTAGAGGGGCATGACGATT+TGG | 0.371552 | 8.4:-19453686 | MS.gene43213:CDS |
| TAGACTTGGAAAGTCGGTTA+TGG | 0.374692 | 8.4:+19454947 | None:intergenic |
| TTTCCTCCTCCGCATCACTA+TGG | 0.374978 | 8.4:+19453614 | None:intergenic |
| TTTAGAAAGAACTGCAGAAA+TGG | 0.378324 | 8.4:-19454300 | MS.gene43213:CDS |
| TAGGATGTTAGATGCACTAT+TGG | 0.386601 | 8.4:-19448159 | MS.gene43213:CDS |
| TTGCACCATTGTTGCATGAA+TGG | 0.389848 | 8.4:-19454122 | MS.gene43213:CDS |
| CTTTATCTAATAAGAAATTC+CGG | 0.393478 | 8.4:-19455254 | MS.gene43213:CDS |
| TTAGAGAATTCTTCTCGAAC+CGG | 0.396247 | 8.4:-19453309 | MS.gene43213:CDS |
| TCTTGGCTCAGCTCAGTTAG+AGG | 0.396277 | 8.4:-19453701 | MS.gene43213:CDS |
| AAAGATGGAGAACTTTAATC+TGG | 0.397162 | 8.4:+19455321 | None:intergenic |
| CTCCTTTATGCAATAGAGAA+TGG | 0.398511 | 8.4:+19454170 | None:intergenic |
| TGGCCTATTGCCTCCAAAGC+TGG | 0.404084 | 8.4:+19453583 | None:intergenic |
| TACTACTGATTCAAAGATAA+GGG | 0.407684 | 8.4:-19453449 | MS.gene43213:CDS |
| GAAATTGAGTCAAGATGATC+TGG | 0.409655 | 8.4:-19452732 | MS.gene43213:CDS |
| AACTCGGCATAAGTTGCAAT+AGG | 0.411380 | 8.4:+19450062 | None:intergenic |
| TACTAAAATGTGTTATAACT+TGG | 0.412883 | 8.4:-19454227 | MS.gene43213:CDS |
| ACACTTCTTGGATGAGCTGA+TGG | 0.415060 | 8.4:-19457108 | MS.gene43213:CDS |
| ATGCAGAGAGAAATCTTTAG+AGG | 0.416654 | 8.4:-19449962 | MS.gene43213:CDS |
| CAAGTGACTTGCTACATATT+GGG | 0.423471 | 8.4:+19449741 | None:intergenic |
| GCAGATTATCTTTCATGCAC+TGG | 0.424257 | 8.4:-19457207 | MS.gene43213:intron |
| CATCAGATCATTTAATTGAC+AGG | 0.426147 | 8.4:-19451401 | MS.gene43213:intron |
| ACTATTTCACGGCAACGGTT+TGG | 0.426162 | 8.4:+19459093 | None:intergenic |
| CTTAACTTCAGAGCAGTGTC+AGG | 0.426892 | 8.4:+19453844 | None:intergenic |
| GATGAGCATGTGGCATTGAT+TGG | 0.431318 | 8.4:+19451237 | None:intergenic |
| AGCAACAAACAAACCTTCAC+TGG | 0.431741 | 8.4:-19455494 | MS.gene43213:CDS |
| TAGTGTTCCTATAATGTTGC+TGG | 0.432236 | 8.4:-19455748 | MS.gene43213:CDS |
| GAATGGGTACAGTATATCTA+AGG | 0.434165 | 8.4:-19453113 | MS.gene43213:CDS |
| CAATGTTGATGACAATGTTA+AGG | 0.435508 | 8.4:-19452918 | MS.gene43213:CDS |
| AGACTTGGAAAGTCGGTTAT+GGG | 0.435699 | 8.4:+19454948 | None:intergenic |
| CTTCTTCTTTCAGCATATGT+TGG | 0.438855 | 8.4:+19457012 | None:intergenic |
| GAAGCTTAACAACTATTTCA+CGG | 0.443297 | 8.4:+19459082 | None:intergenic |
| GTCCATTCTCTATTGCATAA+AGG | 0.444100 | 8.4:-19454172 | MS.gene43213:CDS |
| CTACTACTGATTCAAAGATA+AGG | 0.446476 | 8.4:-19453450 | MS.gene43213:CDS |
| AGAATACAATGTTTGATGAA+TGG | 0.448565 | 8.4:-19453150 | MS.gene43213:CDS |
| ATTCTCTTCTCAAGAAATTG+AGG | 0.449296 | 8.4:-19454782 | MS.gene43213:intron |
| AGCATCGAAGATTTGAAAAC+GGG | 0.451553 | 8.4:+19459212 | None:intergenic |
| CGGGAGCAAGGGAAGATCAT+AGG | 0.451871 | 8.4:-19453289 | MS.gene43213:CDS |
| TCTTGCAGTGTTGTTTCCAT+TGG | 0.452534 | 8.4:+19455645 | None:intergenic |
| TTGTAAGATGGATGTTTCCC+TGG | 0.454024 | 8.4:+19451531 | None:intergenic |
| CTCTTCCTCCATATCCGATA+AGG | 0.465904 | 8.4:+19459281 | None:intergenic |
| CATGCACTCAACATGTTTCA+AGG | 0.467455 | 8.4:-19449866 | MS.gene43213:intron |
| AGATATGATACCTTGGCTAA+TGG | 0.469441 | 8.4:-19453230 | MS.gene43213:CDS |
| TAAGGCGTCATCATTTAATC+AGG | 0.470772 | 8.4:-19453414 | MS.gene43213:CDS |
| TTGAACATAACAGCACTAAT+GGG | 0.471886 | 8.4:-19457148 | MS.gene43213:CDS |
| CAATGCAGTACACAAGTTCT+CGG | 0.475451 | 8.4:+19457057 | None:intergenic |
| GAGGAACTTCAAGGTTCTCA+TGG | 0.475468 | 8.4:-19455455 | MS.gene43213:CDS |
| AAATTAATTCCTGTGGAGTA+TGG | 0.476959 | 8.4:+19453880 | None:intergenic |
| TACCATAAATGCCCTTGTTG+TGG | 0.478121 | 8.4:-19447943 | MS.gene43213:CDS |
| ATTGTCATCAACATTGTTGT+CGG | 0.478504 | 8.4:+19452925 | None:intergenic |
| GGTTAGGTGAATGGTGGAAT+GGG | 0.478929 | 8.4:-19453129 | MS.gene43213:CDS |
| TTGAATGAACTCGGACCAAA+TGG | 0.479156 | 8.4:+19455413 | None:intergenic |
| TTGATGAATGGTTAGGTGAA+TGG | 0.479760 | 8.4:-19453138 | MS.gene43213:CDS |
| TTTAATGTTCTTATGGTCCA+TGG | 0.480913 | 8.4:-19453179 | MS.gene43213:CDS |
| GCTAATGGCATCTTTAACAC+AGG | 0.481517 | 8.4:-19453215 | MS.gene43213:CDS |
| TAATGGCGTTATGCCAAAGA+TGG | 0.482078 | 8.4:+19455306 | None:intergenic |
| GAATTTGCTGCATGAAGTCT+TGG | 0.482807 | 8.4:+19453814 | None:intergenic |
| AACTTCATGTTTGTGATCAA+AGG | 0.486890 | 8.4:+19453534 | None:intergenic |
| ATCCAAATATTTGTCAGAAG+AGG | 0.487452 | 8.4:-19453035 | MS.gene43213:CDS |
| AAGATGTATTCATGACAAAT+CGG | 0.488675 | 8.4:+19449931 | None:intergenic |
| CACTTCTTGGATGAGCTGAT+GGG | 0.490110 | 8.4:-19457107 | MS.gene43213:CDS |
| CTATGATCTTCCCTTGCTCC+CGG | 0.490486 | 8.4:+19453290 | None:intergenic |
| CAATATCAAAAGCGATGGAC+AGG | 0.491085 | 8.4:-19451559 | MS.gene43213:CDS |
| TTTCAGAGTGAGGAACTTCA+AGG | 0.493318 | 8.4:-19455464 | MS.gene43213:CDS |
| AAGTCTACTGAACATTTCCT+TGG | 0.493469 | 8.4:-19454931 | MS.gene43213:CDS |
| AGGAGGAGATCCTTCCTTAT+CGG | 0.493689 | 8.4:-19459295 | MS.gene43213:CDS |
| AATGACAAAGAAACAGAAGA+TGG | 0.493756 | 8.4:-19455350 | MS.gene43213:CDS |
| CAACTTATGCCGAGTTGGAA+AGG | 0.494734 | 8.4:-19450055 | MS.gene43213:CDS |
| AACTTATGCCGAGTTGGAAA+GGG | 0.495045 | 8.4:-19450054 | MS.gene43213:CDS |
| TGGTTAGGTGAATGGTGGAA+TGG | 0.495588 | 8.4:-19453130 | MS.gene43213:CDS |
| TTCCCAGCTTTGGAGGCAAT+AGG | 0.498064 | 8.4:-19453586 | MS.gene43213:CDS |
| CAACCATATATTTGTTCATG+AGG | 0.499363 | 8.4:+19451504 | None:intergenic |
| GGATATGGAGGAAGAGGAGG+AGG | 0.499403 | 8.4:-19459274 | MS.gene43213:CDS |
| GAGCATCGAAGATTTGAAAA+CGG | 0.502621 | 8.4:+19459211 | None:intergenic |
| TGACTCTTGCCTAGACCCTC+AGG | 0.504189 | 8.4:-19452684 | MS.gene43213:CDS |
| GAGGGCTTTCACTGGAGAGC+AGG | 0.504325 | 8.4:+19449903 | None:intergenic |
| AAGATCATAGGATGCATACT+TGG | 0.504340 | 8.4:-19453277 | MS.gene43213:CDS |
| ATCTGGAGAAGGTAATAAGA+AGG | 0.505001 | 8.4:-19452715 | MS.gene43213:CDS |
| CAAGTTCAGAACGCAGAGCT+TGG | 0.505710 | 8.4:+19459163 | None:intergenic |
| AAATTTGTAGAGAAGCTTTG+CGG | 0.506688 | 8.4:-19454841 | MS.gene43213:CDS |
| TTCTTCTCGAACCGGGAGCA+AGG | 0.507447 | 8.4:-19453301 | MS.gene43213:CDS |
| CACCCAAACCCTTTCCAACT+CGG | 0.508340 | 8.4:+19450046 | None:intergenic |
| TAATCAGGAAGCTGCAAGAA+AGG | 0.512537 | 8.4:-19453399 | MS.gene43213:CDS |
| CTTGGCTCAGCTCAGTTAGA+GGG | 0.516578 | 8.4:-19453700 | MS.gene43213:CDS |
| AAGGAAATGTTCAGTAGACT+TGG | 0.518193 | 8.4:+19454933 | None:intergenic |
| AAGGAGCAGCTCCCTTCCAC+TGG | 0.518592 | 8.4:-19447969 | MS.gene43213:CDS |
| CATTGTATTCTTAGTAGCCA+TGG | 0.519388 | 8.4:+19453162 | None:intergenic |
| CCATGGCAAAATATTACTGC+GGG | 0.524020 | 8.4:-19451194 | MS.gene43213:CDS |
| AGAAAATGAAGGAAGCTTGT+GGG | 0.524602 | 8.4:-19454008 | MS.gene43213:CDS |
| AATCCATAGTGATGCGGAGG+AGG | 0.526381 | 8.4:-19453617 | MS.gene43213:CDS |
| CCACATGGGAGGGCTTTCAC+TGG | 0.526805 | 8.4:+19449895 | None:intergenic |
| ACTCGGCATAAGTTGCAATA+GGG | 0.529060 | 8.4:+19450063 | None:intergenic |
| TGTAATGACTGTGAAAAGAA+AGG | 0.530218 | 8.4:-19447988 | MS.gene43213:CDS |
| TAGAGAATTCTTCTCGAACC+GGG | 0.530378 | 8.4:-19453308 | MS.gene43213:CDS |
| CTTATCGGATATGGAGGAAG+AGG | 0.531600 | 8.4:-19459280 | MS.gene43213:CDS |
| AATAGTGTTCCCAGCTTTGG+AGG | 0.531830 | 8.4:-19453593 | MS.gene43213:CDS |
| AAACATGAAGTTGAGCACTT+TGG | 0.533442 | 8.4:-19453523 | MS.gene43213:CDS |
| GGCCTATTGCCTCCAAAGCT+GGG | 0.536547 | 8.4:+19453584 | None:intergenic |
| GCAAGATATTTGAAGATGAA+AGG | 0.537490 | 8.4:-19451167 | MS.gene43213:intron |
| CACAAGTTCTCGGAACAGCT+TGG | 0.538274 | 8.4:+19457067 | None:intergenic |
| AGCGATGGACAGGAATTCCC+AGG | 0.541892 | 8.4:-19451549 | MS.gene43213:CDS |
| AAAAGACCAACCTCGTAAGT+AGG | 0.543105 | 8.4:+19452639 | None:intergenic |
| TTCTTGCCGCAATTTATCCA+TGG | 0.543356 | 8.4:-19451211 | MS.gene43213:CDS |
| AAACAAACGAAGAGAAGTAT+AGG | 0.546504 | 8.4:+19459189 | None:intergenic |
| TATTGCAACTTATGCCGAGT+TGG | 0.549331 | 8.4:-19450060 | MS.gene43213:CDS |
| GTTCAGAACGCAGAGCTTGG+TGG | 0.550240 | 8.4:+19459166 | None:intergenic |
| CCAATATGTAGCAAGTCACT+TGG | 0.552811 | 8.4:-19449740 | MS.gene43213:CDS |
| ATGACAAAGAAACAGAAGAT+GGG | 0.553658 | 8.4:-19455349 | MS.gene43213:CDS |
| GTGGCTCAACATTCTGTAGA+GGG | 0.554576 | 8.4:+19453068 | None:intergenic |
| GAGAAAATGAAGGAAGCTTG+TGG | 0.556774 | 8.4:-19454009 | MS.gene43213:CDS |
| TATGCTGAAAGAAGAAGAGC+AGG | 0.558732 | 8.4:-19457006 | MS.gene43213:intron |
| ACAATGTTTGATGAATGGTT+AGG | 0.559976 | 8.4:-19453145 | MS.gene43213:CDS |
| GACGACGCAGTTTCAGCAAA+CGG | 0.560323 | 8.4:+19459129 | None:intergenic |
| AGCATGCATATGATGCCACT+TGG | 0.561365 | 8.4:-19454252 | MS.gene43213:CDS |
| TTTATGGTAAAGCCAGTGGA+AGG | 0.563649 | 8.4:+19447957 | None:intergenic |
| TCATGAGGATCATTGTAAGA+TGG | 0.564132 | 8.4:+19451519 | None:intergenic |
| TGTAAGATGGATGTTTCCCT+GGG | 0.565215 | 8.4:+19451532 | None:intergenic |
| CAAGTCACTTGGAGACATGC+AGG | 0.566640 | 8.4:-19449729 | MS.gene43213:intron |
| AGTATATCTAAGGCAGCAGA+TGG | 0.566827 | 8.4:-19453103 | MS.gene43213:CDS |
| TGACTTGCTACATATTGGGC+AGG | 0.571852 | 8.4:+19449745 | None:intergenic |
| AATGTATGCAAGCATGAAGT+TGG | 0.571867 | 8.4:+19451436 | None:intergenic |
| ATGTTTGTGATCAAAGGAGT+AGG | 0.573241 | 8.4:+19453540 | None:intergenic |
| TTGCATGAATGGTTCCGCAT+CGG | 0.574198 | 8.4:-19454111 | MS.gene43213:CDS |
| CATTCTCTATTGCATAAAGG+AGG | 0.574541 | 8.4:-19454169 | MS.gene43213:CDS |
| CTCAATTGAAAAGTTCCGAC+AGG | 0.574927 | 8.4:-19454073 | MS.gene43213:CDS |
| TCTTCTCGAACCGGGAGCAA+GGG | 0.576700 | 8.4:-19453300 | MS.gene43213:CDS |
| ACTGAGCTGAGCCAAGAACT+AGG | 0.580216 | 8.4:+19453707 | None:intergenic |
| CAGCATATGTTGGTAGACTG+AGG | 0.580581 | 8.4:+19457022 | None:intergenic |
| CTGCTTGTTTATGTGCTGAA+AGG | 0.581336 | 8.4:+19455697 | None:intergenic |
| ATAAACAAGCAGAAGTTACT+CGG | 0.582541 | 8.4:-19455686 | MS.gene43213:CDS |
| TGACTTGTCATAGTGCCCAC+TGG | 0.582686 | 8.4:+19452806 | None:intergenic |
| TCTGGAGAAGGTAATAAGAA+GGG | 0.582890 | 8.4:-19452714 | MS.gene43213:CDS |
| TTGGCTCAGCTCAGTTAGAG+GGG | 0.583051 | 8.4:-19453699 | MS.gene43213:CDS |
| AAGAACAAGCCTCGCTTGTG+TGG | 0.584106 | 8.4:-19455782 | MS.gene43213:CDS |
| CGGAACCATTCATGCAACAA+TGG | 0.584260 | 8.4:+19454117 | None:intergenic |
| TAACAACTATTTCACGGCAA+CGG | 0.584286 | 8.4:+19459088 | None:intergenic |
| TATTTGGATATAATTTCTAG+TGG | 0.585996 | 8.4:+19453049 | None:intergenic |
| TTGTTTATGTGCTGAAAGGA+AGG | 0.586249 | 8.4:+19455701 | None:intergenic |
| GTTCAGTAGACTTGGAAAGT+CGG | 0.586659 | 8.4:+19454941 | None:intergenic |
| AATGATCTGATGCCTCATCA+TGG | 0.588389 | 8.4:+19451412 | None:intergenic |
| GGAGGAGGAAAACGATGTCA+TGG | 0.589865 | 8.4:-19459259 | MS.gene43213:CDS |
| ATGAATGGTTAGGTGAATGG+TGG | 0.590779 | 8.4:-19453135 | MS.gene43213:CDS |
| GAAGCCAATATCAAAAGCGA+TGG | 0.591818 | 8.4:-19451564 | MS.gene43213:CDS |
| ATGGTTGCAAACACTACAAG+AGG | 0.592631 | 8.4:-19451488 | MS.gene43213:CDS |
| AATGGAAACAACACTGCAAG+AGG | 0.596748 | 8.4:-19455643 | MS.gene43213:intron |
| TATGTGCAAATTAATTCCTG+TGG | 0.596921 | 8.4:+19453873 | None:intergenic |
| TATTACAAGATATGATACCT+TGG | 0.597063 | 8.4:-19453237 | MS.gene43213:CDS |
| GCATGGCATGACCACATGGG+AGG | 0.597202 | 8.4:+19449884 | None:intergenic |
| CATGGCATGACCACATGGGA+GGG | 0.598751 | 8.4:+19449885 | None:intergenic |
| AGTGGCTCAACATTCTGTAG+AGG | 0.601367 | 8.4:+19453067 | None:intergenic |
| GAGTCAAGATGATCTGGAGA+AGG | 0.601856 | 8.4:-19452726 | MS.gene43213:CDS |
| TCCTTCCTTATCGGATATGG+AGG | 0.602235 | 8.4:-19459286 | MS.gene43213:CDS |
| GGCATTTATGGTAAAGCCAG+TGG | 0.602434 | 8.4:+19447953 | None:intergenic |
| CTGAAAATATGTCCCAGTGA+AGG | 0.605019 | 8.4:+19455481 | None:intergenic |
| TAAGGGACAAGAGAGTGCTA+AGG | 0.607113 | 8.4:-19453432 | MS.gene43213:CDS |
| GAGTGCATGGCATGACCACA+TGG | 0.608684 | 8.4:+19449880 | None:intergenic |
| ACGGTTTGGATCATGTTCCA+AGG | 0.612507 | 8.4:+19459107 | None:intergenic |
| ATCGGATATGGAGGAAGAGG+AGG | 0.612689 | 8.4:-19459277 | MS.gene43213:CDS |
| AAGCAGCCACATAAAAGTTG+TGG | 0.612852 | 8.4:-19453964 | MS.gene43213:CDS |
| CTTGAAACATGTTGAGTGCA+TGG | 0.613846 | 8.4:+19449867 | None:intergenic |
| ACATATGCTGCAAGTCCTGT+CGG | 0.614416 | 8.4:+19454058 | None:intergenic |
| TGTGATCAAAGGAGTAGGCA+TGG | 0.615869 | 8.4:+19453545 | None:intergenic |
| TCCTCCATATCCGATAAGGA+AGG | 0.618279 | 8.4:+19459285 | None:intergenic |
| TCTTCTGACTAATGATCCAA+CGG | 0.618296 | 8.4:+19451598 | None:intergenic |
| TATGAGACACCATACTCCAC+AGG | 0.618586 | 8.4:-19453889 | MS.gene43213:CDS |
| GTTAAAGATGCCATTAGCCA+AGG | 0.619614 | 8.4:+19453220 | None:intergenic |
| TGACAAAGAAACAGAAGATG+GGG | 0.619682 | 8.4:-19455348 | MS.gene43213:CDS |
| GTGTTATAGGAACCACAACA+AGG | 0.620988 | 8.4:+19447931 | None:intergenic |
| GAAAGGAAGGATACCATCCA+TGG | 0.624285 | 8.4:+19455714 | None:intergenic |
| TCATTGCGCTGCTGAAGATG+AGG | 0.625736 | 8.4:-19459022 | MS.gene43213:intron |
| CCAAATCCATAGTGATGCGG+AGG | 0.625818 | 8.4:-19453620 | MS.gene43213:CDS |
| TAGTTCCTATTGAATGAACT+CGG | 0.626105 | 8.4:+19455404 | None:intergenic |
| ATTCTCTATTGCATAAAGGA+GGG | 0.628958 | 8.4:-19454168 | MS.gene43213:CDS |
| TGCAATGAAGTAAAACCAGT+GGG | 0.629425 | 8.4:-19452821 | MS.gene43213:CDS |
| AGTATTACCTGTAAATGATG+AGG | 0.629939 | 8.4:+19455171 | None:intergenic |
| TCCATGGCAAAATATTACTG+CGG | 0.629983 | 8.4:-19451195 | MS.gene43213:CDS |
| TATGCAATAGAGAATGGACC+TGG | 0.633980 | 8.4:+19454176 | None:intergenic |
| GCAAACATCTATGCTCACAC+TGG | 0.639385 | 8.4:+19447890 | None:intergenic |
| TTAACTTCAGAGCAGTGTCA+GGG | 0.642545 | 8.4:+19453845 | None:intergenic |
| GCAACAAACAAACCTTCACT+GGG | 0.643067 | 8.4:-19455493 | MS.gene43213:CDS |
| GCATGCATATGATGCCACTT+GGG | 0.646181 | 8.4:-19454251 | MS.gene43213:CDS |
| TGTTCCTATAATGTTGCTGG+AGG | 0.651755 | 8.4:-19455745 | MS.gene43213:CDS |
| CTTCGATGTGACTTTCACCA+AGG | 0.654694 | 8.4:+19454914 | None:intergenic |
| GCCAAATTTCTATTTCCTCG+CGG | 0.657500 | 8.4:+19453338 | None:intergenic |
| TATGAATTGCCACACAAGCG+AGG | 0.661518 | 8.4:+19455773 | None:intergenic |
| TAATACAGAGCCTACTTACG+AGG | 0.662199 | 8.4:-19452649 | MS.gene43213:intron |
| TTATGGTAAAGCCAGTGGAA+GGG | 0.665469 | 8.4:+19447958 | None:intergenic |
| AAATACAAAGAAGCTAGGAG+AGG | 0.669375 | 8.4:+19447868 | None:intergenic |
| CTGCAATGAAGTAAAACCAG+TGG | 0.669462 | 8.4:-19452822 | MS.gene43213:CDS |
| GCGATGGACAGGAATTCCCA+GGG | 0.675192 | 8.4:-19451548 | MS.gene43213:CDS |
| GCATGAAGTTGGTTGCAACA+GGG | 0.676231 | 8.4:+19451447 | None:intergenic |
| GCAAGAAACTGATGAGCATG+TGG | 0.677045 | 8.4:+19451227 | None:intergenic |
| TCACCAAATCCATAGTGATG+CGG | 0.678375 | 8.4:-19453623 | MS.gene43213:CDS |
| TGGCTCAACATTCTGTAGAG+GGG | 0.680577 | 8.4:+19453069 | None:intergenic |
| TTGCATACATTGCCATGATG+AGG | 0.681585 | 8.4:-19451424 | MS.gene43213:CDS |
| CCAGTGAAAGCCCTCCCATG+TGG | 0.682879 | 8.4:-19449895 | MS.gene43213:CDS |
| TGTTATAGGAACCACAACAA+GGG | 0.694348 | 8.4:+19447932 | None:intergenic |
| TTCTGATCATATAAACCGCG+AGG | 0.701161 | 8.4:-19453353 | MS.gene43213:CDS |
| AGTGCATGGCATGACCACAT+GGG | 0.787322 | 8.4:+19449881 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 19447881 | 19459322 | 19447881 | ID=MS.gene43213 |
| chr8.4 | mRNA | 19447881 | 19459322 | 19447881 | ID=MS.gene43213.t1;Parent=MS.gene43213 |
| chr8.4 | exon | 19459023 | 19459322 | 19459023 | ID=MS.gene43213.t1.exon1;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19459023 | 19459322 | 19459023 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19457007 | 19457225 | 19457007 | ID=MS.gene43213.t1.exon2;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19457007 | 19457225 | 19457007 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19455644 | 19455838 | 19455644 | ID=MS.gene43213.t1.exon3;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19455644 | 19455838 | 19455644 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19455179 | 19455537 | 19455179 | ID=MS.gene43213.t1.exon4;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19455179 | 19455537 | 19455179 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19454783 | 19455014 | 19454783 | ID=MS.gene43213.t1.exon5;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19454783 | 19455014 | 19454783 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19452650 | 19454334 | 19452650 | ID=MS.gene43213.t1.exon6;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19452650 | 19454334 | 19452650 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19451402 | 19451620 | 19451402 | ID=MS.gene43213.t1.exon7;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19451402 | 19451620 | 19451402 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19451168 | 19451305 | 19451168 | ID=MS.gene43213.t1.exon8;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19451168 | 19451305 | 19451168 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19449867 | 19450101 | 19449867 | ID=MS.gene43213.t1.exon9;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19449867 | 19450101 | 19449867 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19449730 | 19449789 | 19449730 | ID=MS.gene43213.t1.exon10;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19449730 | 19449789 | 19449730 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19448107 | 19448189 | 19448107 | ID=MS.gene43213.t1.exon11;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19448107 | 19448189 | 19448107 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| chr8.4 | exon | 19447881 | 19448019 | 19447881 | ID=MS.gene43213.t1.exon12;Parent=MS.gene43213.t1 |
| chr8.4 | CDS | 19447881 | 19448019 | 19447881 | ID=cds.MS.gene43213.t1;Parent=MS.gene43213.t1 |
| Gene Sequence |
| Protein sequence |
