AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44265

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44265.t1 MTR_5g032420 98.758 322 4 0 1 322 1 322 0.0 648
MS.gene44265.t1 MTR_8g068890 80.745 322 55 4 1 322 1 315 7.46e-177 492
MS.gene44265.t1 MTR_3g100570 75.229 327 62 7 1 322 1 313 5.97e-169 472
MS.gene44265.t1 MTR_8g086070 37.695 321 160 3 3 322 18 299 2.53e-68 216
MS.gene44265.t1 MTR_7g066690 36.508 315 169 3 6 320 17 300 5.53e-66 210
MS.gene44265.t1 MTR_4g018750 35.647 317 173 3 6 322 15 300 5.16e-64 204
MS.gene44265.t1 MTR_1g090330 32.362 309 177 3 11 318 19 296 7.87e-54 178
MS.gene44265.t1 MTR_3g462140 33.019 318 181 3 4 320 13 299 6.41e-52 173
MS.gene44265.t1 MTR_4g018750 33.445 299 164 5 6 301 15 281 3.24e-47 160
MS.gene44265.t1 MTR_4g018750 28.814 177 113 5 150 317 31 203 3.67e-14 72.0
MS.gene44265.t1 MTR_8g086070 38.356 219 96 2 3 220 18 198 2.90e-45 153
MS.gene44265.t1 MTR_1g088995 30.671 313 178 6 6 318 33 306 5.27e-41 145
MS.gene44265.t1 MTR_1g088995 29.258 229 124 5 6 234 33 223 4.80e-21 90.5
MS.gene44265.t1 MTR_4g057850 25.868 317 187 9 7 315 23 299 1.52e-19 87.8
MS.gene44265.t1 MTR_4g057850 26.087 184 129 2 127 309 15 192 2.59e-11 63.9
MS.gene44265.t1 MTR_4g012850 22.327 318 202 7 6 319 16 292 3.17e-16 78.2
MS.gene44265.t1 MTR_3g104500 23.922 255 178 7 73 320 77 322 5.07e-16 77.8
MS.gene44265.t1 MTR_5g009500 24.047 341 198 9 3 319 35 338 1.05e-15 77.0
MS.gene44265.t1 MTR_5g009500 26.396 197 134 4 131 319 31 224 9.96e-11 62.4
MS.gene44265.t1 MTR_7g117030 25.076 327 216 11 12 321 23 337 2.68e-15 75.9
MS.gene44265.t1 MTR_7g117030 26.549 226 127 7 134 322 14 237 2.52e-14 73.2
MS.gene44265.t1 MTR_2g102010 23.529 323 201 7 4 321 15 296 6.02e-15 74.3
MS.gene44265.t1 MTR_5g055410 26.316 304 183 7 22 319 37 305 9.41e-15 74.3
MS.gene44265.t1 MTR_8g027975 24.854 342 196 11 3 319 48 353 9.91e-15 74.3
MS.gene44265.t1 MTR_8g027975 26.633 199 131 4 131 319 44 237 3.15e-12 67.0
MS.gene44265.t1 MTR_1g087840 25.249 301 190 10 22 319 35 303 2.25e-13 70.1
MS.gene44265.t1 MTR_5g073040 25.538 325 182 9 11 322 40 317 2.35e-13 70.1
MS.gene44265.t1 MTR_6g034515 25.574 305 170 11 21 316 82 338 2.94e-13 70.1
MS.gene44265.t1 MTR_1g097890 27.586 232 150 5 90 319 89 304 4.67e-13 68.9
MS.gene44265.t1 MTR_4g119770 21.765 340 216 9 5 322 17 328 6.10e-13 68.9
MS.gene44265.t1 MTR_4g088545 27.225 191 130 4 131 316 9 195 1.26e-12 67.0
MS.gene44265.t1 MTR_7g064980 21.277 329 217 7 3 322 22 317 1.58e-12 67.8
MS.gene44265.t1 MTR_5g030530 24.611 321 194 9 9 319 14 296 1.67e-12 67.4
MS.gene44265.t1 MTR_5g030530 23.902 205 128 5 132 319 6 199 8.29e-11 62.4
MS.gene44265.t1 MTR_4g088545 27.225 191 130 4 131 316 9 195 1.79e-12 67.0
MS.gene44265.t1 MTR_4g088545 27.225 191 130 4 131 316 9 195 2.56e-12 67.0
MS.gene44265.t1 MTR_6g091800 24.519 208 127 6 128 317 12 207 5.00e-12 65.5
MS.gene44265.t1 MTR_2g098070 25.214 234 160 7 67 293 102 327 6.32e-12 66.2
MS.gene44265.t1 MTR_6g091800 24.519 208 127 6 128 317 12 207 1.12e-11 65.1
MS.gene44265.t1 MTR_7g068340 27.586 174 115 4 150 321 103 267 1.68e-11 65.1
MS.gene44265.t1 MTR_7g109135 23.511 319 201 8 8 319 24 306 1.76e-11 64.3
MS.gene44265.t1 MTR_8g076760 26.908 249 162 9 85 320 65 306 5.96e-11 62.8
MS.gene44265.t1 MTR_8g066975 25.776 322 189 10 9 319 14 296 6.15e-11 62.8
MS.gene44265.t1 MTR_7g083790 27.717 184 114 5 137 315 85 254 7.71e-11 62.8
MS.gene44265.t1 MTR_3g090820 23.596 356 220 16 4 319 43 386 8.75e-11 62.8
MS.gene44265.t1 MTR_3g028590 23.355 304 189 9 6 300 97 365 9.56e-11 62.8
MS.gene44265.t1 MTR_3g028590 23.355 304 189 9 6 300 93 361 9.63e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44265.t1 AT4G24570 73.602 322 76 4 1 322 1 313 5.27e-167 467
MS.gene44265.t1 AT2G22500 74.613 323 69 6 1 322 1 311 9.10e-163 456
MS.gene44265.t1 AT5G09470 59.531 341 111 8 1 321 1 334 5.17e-123 356
MS.gene44265.t1 AT5G19760 38.272 324 150 4 3 320 15 294 9.07e-70 219
MS.gene44265.t1 AT3G54110 36.364 319 172 4 4 322 13 300 2.21e-66 211
MS.gene44265.t1 AT5G58970 36.000 325 159 5 6 319 12 298 3.40e-65 207
MS.gene44265.t1 AT5G58970 26.222 225 115 7 9 228 127 305 1.25e-12 67.8
MS.gene44265.t1 AT1G14140 33.119 311 173 5 11 318 21 299 2.15e-52 175
MS.gene44265.t1 AT4G03115 32.075 318 170 6 3 318 35 308 2.20e-47 162
MS.gene44265.t1 AT4G03115 32.075 318 170 6 3 318 67 340 3.71e-47 162
MS.gene44265.t1 AT5G58970 35.385 260 126 4 6 254 12 240 5.55e-45 154
MS.gene44265.t1 AT5G58970 26.012 173 123 3 150 317 32 204 4.57e-16 77.4
MS.gene44265.t1 AT4G03115 33.462 260 154 5 61 318 3 245 5.09e-43 149
MS.gene44265.t1 AT4G03115 33.884 242 144 4 79 318 10 237 5.55e-41 143
MS.gene44265.t1 AT4G03115 28.879 232 124 4 3 234 35 225 3.89e-25 101
MS.gene44265.t1 AT4G03115 28.879 232 124 4 3 234 35 225 4.26e-25 101
MS.gene44265.t1 AT5G01340 26.562 320 192 8 7 320 18 300 1.26e-21 93.6
MS.gene44265.t1 AT5G01340 26.702 191 133 2 120 309 3 187 3.46e-12 66.6
MS.gene44265.t1 AT5G46800 26.235 324 188 9 9 319 14 299 2.11e-17 81.6
MS.gene44265.t1 AT5G46800 22.222 198 145 2 131 319 5 202 2.53e-12 67.0
MS.gene44265.t1 AT5G46800 26.235 324 188 9 9 319 14 299 2.11e-17 81.6
MS.gene44265.t1 AT5G46800 22.222 198 145 2 131 319 5 202 2.53e-12 67.0
MS.gene44265.t1 AT5G46800 26.235 324 188 9 9 319 14 299 2.11e-17 81.6
MS.gene44265.t1 AT5G46800 22.222 198 145 2 131 319 5 202 2.53e-12 67.0
MS.gene44265.t1 AT5G56450 25.969 258 169 7 73 320 81 326 1.22e-16 79.7
MS.gene44265.t1 AT1G25380 25.413 303 190 7 22 320 37 307 1.29e-14 74.3
MS.gene44265.t1 AT1G25380 25.413 303 190 7 22 320 37 307 1.29e-14 74.3
MS.gene44265.t1 AT1G07030 26.687 326 188 9 6 322 36 319 2.36e-14 73.2
MS.gene44265.t1 AT2G33820 24.925 333 193 13 5 319 13 306 1.80e-13 70.5
MS.gene44265.t1 AT4G01100 24.854 342 194 11 4 319 40 344 2.17e-13 70.5
MS.gene44265.t1 AT4G01100 26.396 197 134 3 131 319 35 228 3.77e-12 66.6
MS.gene44265.t1 AT4G27940 23.362 351 214 11 12 320 71 408 4.19e-13 70.1
MS.gene44265.t1 AT3G51870 30.286 175 109 5 150 321 107 271 7.37e-13 68.9
MS.gene44265.t1 AT3G51870 22.848 302 185 9 20 319 105 360 6.37e-12 66.2
MS.gene44265.t1 AT4G26180 27.451 204 128 7 127 317 13 209 7.72e-13 68.2
MS.gene44265.t1 AT4G01100 26.415 265 165 8 80 319 35 294 8.24e-13 68.2
MS.gene44265.t1 AT3G53940 23.423 333 197 9 3 319 70 360 1.04e-12 68.6
MS.gene44265.t1 AT3G53940 24.870 193 128 4 138 318 73 260 1.36e-12 67.4
MS.gene44265.t1 AT4G26180 27.451 204 128 7 127 317 13 209 1.50e-12 67.8
MS.gene44265.t1 AT3G55640 24.103 195 133 3 138 321 38 228 2.73e-12 66.2
MS.gene44265.t1 AT3G55640 24.103 195 133 3 138 321 38 228 2.73e-12 66.2
MS.gene44265.t1 AT4G01100 24.490 343 210 14 3 319 39 358 3.07e-12 67.0
MS.gene44265.t1 AT5G01500 27.528 178 118 4 146 321 131 299 4.03e-12 67.0
MS.gene44265.t1 AT5G01500 24.000 300 184 7 20 319 133 388 2.00e-11 64.7
MS.gene44265.t1 AT5G66380 28.718 195 121 8 136 322 12 196 4.64e-12 66.2
MS.gene44265.t1 AT5G17400 22.712 295 188 7 6 293 14 275 9.14e-12 65.5
MS.gene44265.t1 AT2G26360 23.220 323 180 11 3 317 112 374 1.13e-11 65.5
MS.gene44265.t1 AT2G39970 23.656 279 178 8 71 317 44 319 1.46e-11 64.7
MS.gene44265.t1 AT4G28390 22.517 302 194 7 6 300 83 351 4.54e-11 63.5
MS.gene44265.t1 AT4G28390 22.517 302 194 7 6 300 83 351 4.54e-11 63.5
MS.gene44265.t1 AT2G35800 23.750 320 187 10 3 320 542 806 4.65e-11 64.3
MS.gene44265.t1 AT2G37890 22.936 327 204 8 4 319 43 332 6.16e-11 63.2
MS.gene44265.t1 AT2G37890 23.711 194 135 3 131 316 42 230 7.54e-11 62.8
MS.gene44265.t1 AT1G79900 23.293 249 172 5 75 319 50 283 6.40e-11 62.8
MS.gene44265.t1 AT2G33820 25.243 206 142 6 116 316 3 201 7.50e-11 62.4
MS.gene44265.t1 AT3G21390 22.659 331 202 9 9 313 22 324 7.97e-11 62.8
MS.gene44265.t1 AT3G21390 22.659 331 202 9 9 313 44 346 9.77e-11 62.4

Find 117 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATGGATCGTGGTTGTTC+CGG 0.314214 5.4:-22701780 None:intergenic
CTCCGACCGGCACTTGCTTT+CGG 0.332288 5.4:+22701752 MS.gene44265:CDS
GGACTTATTGCCGGCGGGGT+TGG 0.357971 5.4:+22702025 MS.gene44265:CDS
TGTTGTTCTGTTTGTTACTT+TGG 0.360137 5.4:+22702531 MS.gene44265:CDS
ACATCCACTAGATCTTATTA+AGG 0.364470 5.4:+22701676 MS.gene44265:CDS
ACCACCTTATGCTGGTGCTT+TGG 0.365642 5.4:+22702420 MS.gene44265:CDS
AGTTCGTGCTGAGGGTCCTT+TGG 0.375226 5.4:+22702459 MS.gene44265:CDS
CGAACCATGGCAACGTCGGC+GGG 0.400237 5.4:-22702064 None:intergenic
GGTGTTATGCGTGATGGGCT+TGG 0.402456 5.4:+22702286 MS.gene44265:CDS
AGGAAGGTGTGACCAGTCTT+TGG 0.402796 5.4:+22702170 MS.gene44265:CDS
CGGAGGATCTGAACCGGGTT+CGG 0.406065 5.4:-22701734 None:intergenic
ATCGAGGCTGGACTTATTGC+CGG 0.411260 5.4:+22702016 MS.gene44265:CDS
CCCACACGAGGCTGAGGAAC+CGG 0.415972 5.4:-22701812 None:intergenic
GGGTCCTTTGGCTCTTTACA+AGG 0.417797 5.4:+22702471 MS.gene44265:CDS
GTGTTATGCGTGATGGGCTT+GGG 0.421903 5.4:+22702287 MS.gene44265:CDS
CTGCTTCGCCATTCTAGTAA+TGG 0.443816 5.4:-22702149 None:intergenic
GCCGGTCGGAGGATCTGAAC+CGG 0.451478 5.4:-22701740 None:intergenic
GGTGTAACAGCTTTGTTCTC+CGG 0.452972 5.4:+22701872 MS.gene44265:CDS
GGCGGGGTTGGTGCTGCTAT+CGG 0.453712 5.4:+22702037 MS.gene44265:CDS
GCATCTCCGCCACTGTGCTC+AGG 0.455298 5.4:+22701894 MS.gene44265:CDS
CTGTTTCGCAAGATCGAGGC+TGG 0.456258 5.4:+22702004 MS.gene44265:CDS
CATTACTAGAATGGCGAAGC+AGG 0.462623 5.4:+22702150 MS.gene44265:CDS
GAGCACAGTGGCGGAGATGC+CGG 0.463595 5.4:-22701891 None:intergenic
AAGGTTCGCATGCAACTTCA+AGG 0.464338 5.4:+22701695 MS.gene44265:CDS
ATCTGAACCGGGTTCGGCTT+AGG 0.465925 5.4:-22701728 None:intergenic
GGTCCCTGCCTTGAAATTGT+AGG 0.469679 5.4:-22702502 None:intergenic
GATTTATTCCTACAATTTCA+AGG 0.470932 5.4:+22702494 MS.gene44265:CDS
GGCGGCTGTTGCGTCGAATC+CGG 0.475424 5.4:+22702345 MS.gene44265:CDS
TATTCCTACAATTTCAAGGC+AGG 0.475430 5.4:+22702498 MS.gene44265:CDS
GCACTTGCTTTCGGTCAAAC+CGG 0.477758 5.4:+22701761 MS.gene44265:CDS
TCCGGTTCCTCAGCCTCGTG+TGG 0.482332 5.4:+22701811 MS.gene44265:CDS
TGATGACCCGCGCCAAAGAC+TGG 0.483708 5.4:-22702182 None:intergenic
GCAAGTTCGCAAGTTGTTGA+AGG 0.493746 5.4:+22702555 MS.gene44265:CDS
AGCCGATGGGAGACTCCCAC+CGG 0.495248 5.4:+22702093 MS.gene44265:CDS
CAATCCAAAGCACCAGCATA+AGG 0.495820 5.4:-22702424 None:intergenic
ATGAACATGAAGGTGGAGGT+TGG 0.497583 5.4:+22702391 MS.gene44265:CDS
CTGCAAAACTAGCTGTCACA+TGG 0.501796 5.4:-22702312 None:intergenic
CAAGGAGATGATTCTTGAGA+AGG 0.502747 5.4:+22702264 MS.gene44265:CDS
GCTGGTCACAGCTTCACAGC+TGG 0.507929 5.4:+22702225 MS.gene44265:CDS
ATAGAGTCCCATCCTGGTGG+TGG 0.515961 5.4:-22701930 None:intergenic
TAGTTTCTCTGCTGAGCCGG+TGG 0.518095 5.4:-22702109 None:intergenic
AAGTGGACAGATCGTGAAGC+TGG 0.518322 5.4:+22701968 MS.gene44265:CDS
AGGCTGGACTTATTGCCGGC+GGG 0.524366 5.4:+22702020 MS.gene44265:CDS
GACTGTGAACCGCGCCATGC+TGG 0.524934 5.4:+22702207 MS.gene44265:CDS
TTGAAGACAGTTCGTGCTGA+GGG 0.525849 5.4:+22702451 MS.gene44265:CDS
TTGTAGTTTCTCTGCTGAGC+CGG 0.527077 5.4:-22702112 None:intergenic
TCGAACCATGGCAACGTCGG+CGG 0.536548 5.4:-22702065 None:intergenic
CATGAAGGTGGAGGTTGGAA+AGG 0.542492 5.4:+22702396 MS.gene44265:CDS
GTGTGACCAGTCTTTGGCGC+GGG 0.545353 5.4:+22702176 MS.gene44265:CDS
CACCAGGATGGGACTCTATG+AGG 0.546356 5.4:+22701934 MS.gene44265:CDS
TAACCCCGCCGACGTTGCCA+TGG 0.547137 5.4:+22702060 MS.gene44265:CDS
CGGTGGATGTTATTAAGACA+AGG 0.547567 5.4:+22702365 MS.gene44265:CDS
AGAAGGGTGTTATGCGTGAT+GGG 0.547920 5.4:+22702281 MS.gene44265:CDS
GGAAAGGAACCACCTTATGC+TGG 0.548867 5.4:+22702412 MS.gene44265:CDS
GAGAAGGGTGTTATGCGTGA+TGG 0.556651 5.4:+22702280 MS.gene44265:CDS
GTCCGTCTTGTTCAACAAGA+AGG 0.557738 5.4:+22701851 MS.gene44265:CDS
CGTGTGGGCCTTATCTCAGT+CGG 0.561147 5.4:+22701827 MS.gene44265:CDS
CACCTTCTTGTTGAACAAGA+CGG 0.561342 5.4:-22701853 None:intergenic
TGTCGACGCCATTACTAGAA+TGG 0.561691 5.4:+22702141 MS.gene44265:CDS
GGCATCATATGATCAGTTCA+AGG 0.562745 5.4:+22702246 MS.gene44265:CDS
AACCTCATAGAGTCCCATCC+TGG 0.563410 5.4:-22701936 None:intergenic
ATGGTTCGAATGCAAGCCGA+TGG 0.563779 5.4:+22702079 MS.gene44265:CDS
AAGGAGATGATTCTTGAGAA+GGG 0.566148 5.4:+22702265 MS.gene44265:CDS
GACGGACTCCGACTGAGATA+AGG 0.567027 5.4:-22701835 None:intergenic
AGTTTCTCTGCTGAGCCGGT+GGG 0.569976 5.4:-22702108 None:intergenic
CTGTATTCCACCACCAGGAT+GGG 0.571102 5.4:+22701923 MS.gene44265:CDS
CTTGTCTTAATAACATCCAC+CGG 0.572366 5.4:-22702364 None:intergenic
ATCGGCTTGCATTCGAACCA+TGG 0.573789 5.4:-22702077 None:intergenic
AGCCGGTGGGAGTCTCCCAT+CGG 0.573898 5.4:-22702095 None:intergenic
GGTCCTTTGGCTCTTTACAA+GGG 0.576211 5.4:+22702472 MS.gene44265:CDS
CCGGTTCCTCAGCCTCGTGT+GGG 0.577991 5.4:+22701812 MS.gene44265:CDS
TTTGAAGACAGTTCGTGCTG+AGG 0.579339 5.4:+22702450 MS.gene44265:CDS
GATCCATGTGGGGTCTACTC+CGG 0.580439 5.4:+22701793 MS.gene44265:CDS
ACAAACAGAACAACAGTGAA+AGG 0.581480 5.4:-22702523 None:intergenic
ATAAGGCCCACACGAGGCTG+AGG 0.584839 5.4:-22701818 None:intergenic
ATTCCTACAATTTCAAGGCA+GGG 0.587080 5.4:+22702499 MS.gene44265:CDS
CGAACCTTAATAAGATCTAG+TGG 0.587146 5.4:-22701680 None:intergenic
GGCTGGACTTATTGCCGGCG+GGG 0.587219 5.4:+22702021 MS.gene44265:CDS
CGAAAGCAAGTGCCGGTCGG+AGG 0.591618 5.4:-22701751 None:intergenic
AATCCCTTGTAAAGAGCCAA+AGG 0.597291 5.4:-22702475 None:intergenic
GGTATTGCTTCAATCATCGC+AGG 0.598971 5.4:+22701644 MS.gene44265:CDS
ACTGTATTCCACCACCAGGA+TGG 0.600318 5.4:+22701922 MS.gene44265:CDS
AATAAGATCTAGTGGATGTG+TGG 0.600430 5.4:-22701672 None:intergenic
CCGGTCGGAGGATCTGAACC+GGG 0.601331 5.4:-22701739 None:intergenic
CCCGGTTCAGATCCTCCGAC+CGG 0.604021 5.4:+22701739 MS.gene44265:CDS
GCCTCGATCTTGCGAAACAG+CGG 0.604552 5.4:-22702001 None:intergenic
GAACCGGAGTAGACCCCACA+TGG 0.607542 5.4:-22701796 None:intergenic
GCCGCTGTTTCGCAAGATCG+AGG 0.616216 5.4:+22702000 MS.gene44265:CDS
GTTTGACCGAAAGCAAGTGC+CGG 0.620216 5.4:-22701758 None:intergenic
GATAGCAGCACCAACCCCGC+CGG 0.621207 5.4:-22702035 None:intergenic
GACCGAAAGCAAGTGCCGGT+CGG 0.622497 5.4:-22701754 None:intergenic
AAACGCGCCTAAGCCGAACC+CGG 0.624848 5.4:+22701721 MS.gene44265:CDS
CATTCGAACCATGGCAACGT+CGG 0.625579 5.4:-22702068 None:intergenic
ACTAGAATGGCGAAGCAGGA+AGG 0.629489 5.4:+22702154 MS.gene44265:CDS
GGAACAACCACGATCCATGT+GGG 0.630425 5.4:+22701782 MS.gene44265:CDS
GGTGTGACCAGTCTTTGGCG+CGG 0.633502 5.4:+22702175 MS.gene44265:CDS
TGGTTCGAATGCAAGCCGAT+GGG 0.641550 5.4:+22702080 MS.gene44265:CDS
TGTCTGCCTGAGCACAGTGG+CGG 0.644365 5.4:-22701900 None:intergenic
CGGAACAACCACGATCCATG+TGG 0.645591 5.4:+22701781 MS.gene44265:CDS
CTGTGAAGCTGTGACCAGCA+TGG 0.646036 5.4:-22702221 None:intergenic
AAGGGTGATGAACATGAAGG+TGG 0.649113 5.4:+22702384 MS.gene44265:CDS
GAGGCTGGACTTATTGCCGG+CGG 0.651692 5.4:+22702019 MS.gene44265:CDS
AGTAGACCCCACATGGATCG+TGG 0.652877 5.4:-22701789 None:intergenic
CTCATAGAGTCCCATCCTGG+TGG 0.654507 5.4:-22701933 None:intergenic
GGCTGTTGCGTCGAATCCGG+TGG 0.655060 5.4:+22702348 MS.gene44265:CDS
TCCAAAGCACCAGCATAAGG+TGG 0.656451 5.4:-22702421 None:intergenic
GAACCATGGCAACGTCGGCG+GGG 0.658264 5.4:-22702063 None:intergenic
AGACACTGTATTCCACCACC+AGG 0.665651 5.4:+22701918 MS.gene44265:CDS
GACAAGGGTGATGAACATGA+AGG 0.670089 5.4:+22702381 MS.gene44265:CDS
GGTGATGAACATGAAGGTGG+AGG 0.674760 5.4:+22702387 MS.gene44265:CDS
TGCAAAACTAGCTGTCACAT+GGG 0.682381 5.4:-22702311 None:intergenic
AAGCTGTGACCAGCATGGCG+CGG 0.685288 5.4:-22702216 None:intergenic
GGTGGATGTTATTAAGACAA+GGG 0.685805 5.4:+22702366 MS.gene44265:CDS
GAACAACCACGATCCATGTG+GGG 0.695252 5.4:+22701783 MS.gene44265:CDS
TGGACAGATCGTGAAGCTGG+CGG 0.696219 5.4:+22701971 MS.gene44265:CDS
ACTGAGATAAGGCCCACACG+AGG 0.739468 5.4:-22701824 None:intergenic
CAGTGTCTGCCTGAGCACAG+TGG 0.748180 5.4:-22701903 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTTATTCCTACAATTTCA+AGG + chr5.4:22702494-22702513 MS.gene44265:CDS 25.0%
ACATCCACTAGATCTTATTA+AGG + chr5.4:22701676-22701695 MS.gene44265:CDS 30.0%
! TGTTGTTCTGTTTGTTACTT+TGG + chr5.4:22702531-22702550 MS.gene44265:CDS 30.0%
AAGGAGATGATTCTTGAGAA+GGG + chr5.4:22702265-22702284 MS.gene44265:CDS 35.0%
ACAAACAGAACAACAGTGAA+AGG - chr5.4:22702526-22702545 None:intergenic 35.0%
ATGAGGTTCTCAAAAACAAG+TGG + chr5.4:22701951-22701970 MS.gene44265:CDS 35.0%
ATTCCTACAATTTCAAGGCA+GGG + chr5.4:22702499-22702518 MS.gene44265:CDS 35.0%
CGAACCTTAATAAGATCTAG+TGG - chr5.4:22701683-22701702 None:intergenic 35.0%
CTTGTCTTAATAACATCCAC+CGG - chr5.4:22702367-22702386 None:intergenic 35.0%
GGTGGATGTTATTAAGACAA+GGG + chr5.4:22702366-22702385 MS.gene44265:CDS 35.0%
TATTCCTACAATTTCAAGGC+AGG + chr5.4:22702498-22702517 MS.gene44265:CDS 35.0%
! AATAAGATCTAGTGGATGTG+TGG - chr5.4:22701675-22701694 None:intergenic 35.0%
AATCCCTTGTAAAGAGCCAA+AGG - chr5.4:22702478-22702497 None:intergenic 40.0%
CAAGGAGATGATTCTTGAGA+AGG + chr5.4:22702264-22702283 MS.gene44265:CDS 40.0%
CACCTTCTTGTTGAACAAGA+CGG - chr5.4:22701856-22701875 None:intergenic 40.0%
CGGTGGATGTTATTAAGACA+AGG + chr5.4:22702365-22702384 MS.gene44265:CDS 40.0%
GGCATCATATGATCAGTTCA+AGG + chr5.4:22702246-22702265 MS.gene44265:CDS 40.0%
TGCAAAACTAGCTGTCACAT+GGG - chr5.4:22702314-22702333 None:intergenic 40.0%
!!! GGTGTCAAAGGTTTTGTTGA+AGG + chr5.4:22701620-22701639 MS.gene44265:CDS 40.0%
!!! GTCAAAGGTTTTGTTGAAGG+TGG + chr5.4:22701623-22701642 MS.gene44265:CDS 40.0%
AAGGGTGATGAACATGAAGG+TGG + chr5.4:22702384-22702403 MS.gene44265:CDS 45.0%
AAGGTTCGCATGCAACTTCA+AGG + chr5.4:22701695-22701714 MS.gene44265:CDS 45.0%
ATGAACATGAAGGTGGAGGT+TGG + chr5.4:22702391-22702410 MS.gene44265:CDS 45.0%
CATTACTAGAATGGCGAAGC+AGG + chr5.4:22702150-22702169 MS.gene44265:CDS 45.0%
CTGCAAAACTAGCTGTCACA+TGG - chr5.4:22702315-22702334 None:intergenic 45.0%
CTGCTTCGCCATTCTAGTAA+TGG - chr5.4:22702152-22702171 None:intergenic 45.0%
GACAAGGGTGATGAACATGA+AGG + chr5.4:22702381-22702400 MS.gene44265:CDS 45.0%
GCAAGTTCGCAAGTTGTTGA+AGG + chr5.4:22702555-22702574 MS.gene44265:CDS 45.0%
GGTATTGCTTCAATCATCGC+AGG + chr5.4:22701644-22701663 MS.gene44265:CDS 45.0%
TGTCGACGCCATTACTAGAA+TGG + chr5.4:22702141-22702160 MS.gene44265:CDS 45.0%
TTGAAGACAGTTCGTGCTGA+GGG + chr5.4:22702451-22702470 MS.gene44265:CDS 45.0%
TTGTAGTTTCTCTGCTGAGC+CGG - chr5.4:22702115-22702134 None:intergenic 45.0%
TTTGAAGACAGTTCGTGCTG+AGG + chr5.4:22702450-22702469 MS.gene44265:CDS 45.0%
! CAATCCAAAGCACCAGCATA+AGG - chr5.4:22702427-22702446 None:intergenic 45.0%
! GTCCGTCTTGTTCAACAAGA+AGG + chr5.4:22701851-22701870 MS.gene44265:CDS 45.0%
!! AGAAGGGTGTTATGCGTGAT+GGG + chr5.4:22702281-22702300 MS.gene44265:CDS 45.0%
!! GGTCCTTTGGCTCTTTACAA+GGG + chr5.4:22702472-22702491 MS.gene44265:CDS 45.0%
!! GGTGTAACAGCTTTGTTCTC+CGG + chr5.4:22701872-22701891 MS.gene44265:CDS 45.0%
!! TAGTTTTGCAGCGGGATTTG+TGG + chr5.4:22702324-22702343 MS.gene44265:CDS 45.0%
!!! TGTGACAGCTAGTTTTGCAG+CGG + chr5.4:22702315-22702334 MS.gene44265:CDS 45.0%
AACCTCATAGAGTCCCATCC+TGG - chr5.4:22701939-22701958 None:intergenic 50.0%
AAGTGGACAGATCGTGAAGC+TGG + chr5.4:22701968-22701987 MS.gene44265:CDS 50.0%
ACCACCTTATGCTGGTGCTT+TGG + chr5.4:22702420-22702439 MS.gene44265:CDS 50.0%
ACTAGAATGGCGAAGCAGGA+AGG + chr5.4:22702154-22702173 MS.gene44265:CDS 50.0%
ACTGTATTCCACCACCAGGA+TGG + chr5.4:22701922-22701941 MS.gene44265:CDS 50.0%
AGACACTGTATTCCACCACC+AGG + chr5.4:22701918-22701937 MS.gene44265:CDS 50.0%
ATCGAGGCTGGACTTATTGC+CGG + chr5.4:22702016-22702035 MS.gene44265:CDS 50.0%
ATGGTTCGAATGCAAGCCGA+TGG + chr5.4:22702079-22702098 MS.gene44265:CDS 50.0%
CACATGGATCGTGGTTGTTC+CGG - chr5.4:22701783-22701802 None:intergenic 50.0%
CATGAAGGTGGAGGTTGGAA+AGG + chr5.4:22702396-22702415 MS.gene44265:CDS 50.0%
CATTCGAACCATGGCAACGT+CGG - chr5.4:22702071-22702090 None:intergenic 50.0%
CTGTATTCCACCACCAGGAT+GGG + chr5.4:22701923-22701942 MS.gene44265:CDS 50.0%
GAACAACCACGATCCATGTG+GGG + chr5.4:22701783-22701802 MS.gene44265:CDS 50.0%
GCACTTGCTTTCGGTCAAAC+CGG + chr5.4:22701761-22701780 MS.gene44265:CDS 50.0%
GGAAAGGAACCACCTTATGC+TGG + chr5.4:22702412-22702431 MS.gene44265:CDS 50.0%
GGAACAACCACGATCCATGT+GGG + chr5.4:22701782-22701801 MS.gene44265:CDS 50.0%
GGTCCCTGCCTTGAAATTGT+AGG - chr5.4:22702505-22702524 None:intergenic 50.0%
GGTGATGAACATGAAGGTGG+AGG + chr5.4:22702387-22702406 MS.gene44265:CDS 50.0%
GTTTGACCGAAAGCAAGTGC+CGG - chr5.4:22701761-22701780 None:intergenic 50.0%
TGGTTCGAATGCAAGCCGAT+GGG + chr5.4:22702080-22702099 MS.gene44265:CDS 50.0%
! AGGAAGGTGTGACCAGTCTT+TGG + chr5.4:22702170-22702189 MS.gene44265:CDS 50.0%
! TCCAAAGCACCAGCATAAGG+TGG - chr5.4:22702424-22702443 None:intergenic 50.0%
! TTTTGCAGCGGGATTTGTGG+CGG + chr5.4:22702327-22702346 MS.gene44265:CDS 50.0%
!! ATCGGCTTGCATTCGAACCA+TGG - chr5.4:22702080-22702099 None:intergenic 50.0%
!! GAGAAGGGTGTTATGCGTGA+TGG + chr5.4:22702280-22702299 MS.gene44265:CDS 50.0%
!! GGGTCCTTTGGCTCTTTACA+AGG + chr5.4:22702471-22702490 MS.gene44265:CDS 50.0%
!! GTGTTATGCGTGATGGGCTT+GGG + chr5.4:22702287-22702306 MS.gene44265:CDS 50.0%
!!! GTGACAGCTAGTTTTGCAGC+GGG + chr5.4:22702316-22702335 MS.gene44265:CDS 50.0%
ACTGAGATAAGGCCCACACG+AGG - chr5.4:22701827-22701846 None:intergenic 55.0%
AGTAGACCCCACATGGATCG+TGG - chr5.4:22701792-22701811 None:intergenic 55.0%
AGTTCGTGCTGAGGGTCCTT+TGG + chr5.4:22702459-22702478 MS.gene44265:CDS 55.0%
AGTTTCTCTGCTGAGCCGGT+GGG - chr5.4:22702111-22702130 None:intergenic 55.0%
ATAGAGTCCCATCCTGGTGG+TGG - chr5.4:22701933-22701952 None:intergenic 55.0%
ATCTGAACCGGGTTCGGCTT+AGG - chr5.4:22701731-22701750 None:intergenic 55.0%
CACCAGGATGGGACTCTATG+AGG + chr5.4:22701934-22701953 MS.gene44265:CDS 55.0%
CGGAACAACCACGATCCATG+TGG + chr5.4:22701781-22701800 MS.gene44265:CDS 55.0%
CTCATAGAGTCCCATCCTGG+TGG - chr5.4:22701936-22701955 None:intergenic 55.0%
CTGTGAAGCTGTGACCAGCA+TGG - chr5.4:22702224-22702243 None:intergenic 55.0%
CTGTTTCGCAAGATCGAGGC+TGG + chr5.4:22702004-22702023 MS.gene44265:CDS 55.0%
GATCCATGTGGGGTCTACTC+CGG + chr5.4:22701793-22701812 MS.gene44265:CDS 55.0%
GCCTCGATCTTGCGAAACAG+CGG - chr5.4:22702004-22702023 None:intergenic 55.0%
TAGTTTCTCTGCTGAGCCGG+TGG - chr5.4:22702112-22702131 None:intergenic 55.0%
TGGACAGATCGTGAAGCTGG+CGG + chr5.4:22701971-22701990 MS.gene44265:CDS 55.0%
! CGTGTGGGCCTTATCTCAGT+CGG + chr5.4:22701827-22701846 MS.gene44265:CDS 55.0%
! GACGGACTCCGACTGAGATA+AGG - chr5.4:22701838-22701857 None:intergenic 55.0%
!! GGTGTTATGCGTGATGGGCT+TGG + chr5.4:22702286-22702305 MS.gene44265:CDS 55.0%
AAACGCGCCTAAGCCGAACC+CGG + chr5.4:22701721-22701740 MS.gene44265:CDS 60.0%
AAGCTGTGACCAGCATGGCG+CGG - chr5.4:22702219-22702238 None:intergenic 60.0%
AGGCTGGACTTATTGCCGGC+GGG + chr5.4:22702020-22702039 MS.gene44265:CDS 60.0%
ATAAGGCCCACACGAGGCTG+AGG - chr5.4:22701821-22701840 None:intergenic 60.0%
CAGTGTCTGCCTGAGCACAG+TGG - chr5.4:22701906-22701925 None:intergenic 60.0%
CGGAGGATCTGAACCGGGTT+CGG - chr5.4:22701737-22701756 None:intergenic 60.0%
CTCCGACCGGCACTTGCTTT+CGG + chr5.4:22701752-22701771 MS.gene44265:CDS 60.0%
GAACCGGAGTAGACCCCACA+TGG - chr5.4:22701799-22701818 None:intergenic 60.0%
GAGGCTGGACTTATTGCCGG+CGG + chr5.4:22702019-22702038 MS.gene44265:CDS 60.0%
GCTGGTCACAGCTTCACAGC+TGG + chr5.4:22702225-22702244 MS.gene44265:CDS 60.0%
GGTGTGACCAGTCTTTGGCG+CGG + chr5.4:22702175-22702194 MS.gene44265:CDS 60.0%
GTGTGACCAGTCTTTGGCGC+GGG + chr5.4:22702176-22702195 MS.gene44265:CDS 60.0%
TCGAACCATGGCAACGTCGG+CGG - chr5.4:22702068-22702087 None:intergenic 60.0%
TGATGACCCGCGCCAAAGAC+TGG - chr5.4:22702185-22702204 None:intergenic 60.0%
TGTCTGCCTGAGCACAGTGG+CGG - chr5.4:22701903-22701922 None:intergenic 60.0%
! GCCGCTGTTTCGCAAGATCG+AGG + chr5.4:22702000-22702019 MS.gene44265:CDS 60.0%
!! GACCGAAAGCAAGTGCCGGT+CGG - chr5.4:22701757-22701776 None:intergenic 60.0%
AGCCGATGGGAGACTCCCAC+CGG + chr5.4:22702093-22702112 MS.gene44265:CDS 65.0%
AGCCGGTGGGAGTCTCCCAT+CGG - chr5.4:22702098-22702117 None:intergenic 65.0%
CCCACACGAGGCTGAGGAAC+CGG - chr5.4:22701815-22701834 None:intergenic 65.0%
CCCGGTTCAGATCCTCCGAC+CGG + chr5.4:22701739-22701758 MS.gene44265:CDS 65.0%
CCGGTCGGAGGATCTGAACC+GGG - chr5.4:22701742-22701761 None:intergenic 65.0%
CCGGTTCCTCAGCCTCGTGT+GGG + chr5.4:22701812-22701831 MS.gene44265:CDS 65.0%
CGAACCATGGCAACGTCGGC+GGG - chr5.4:22702067-22702086 None:intergenic 65.0%
GAACCATGGCAACGTCGGCG+GGG - chr5.4:22702066-22702085 None:intergenic 65.0%
GACTGTGAACCGCGCCATGC+TGG + chr5.4:22702207-22702226 MS.gene44265:CDS 65.0%
GATAGCAGCACCAACCCCGC+CGG - chr5.4:22702038-22702057 None:intergenic 65.0%
GCATCTCCGCCACTGTGCTC+AGG + chr5.4:22701894-22701913 MS.gene44265:CDS 65.0%
GCCGGTCGGAGGATCTGAAC+CGG - chr5.4:22701743-22701762 None:intergenic 65.0%
GGACTTATTGCCGGCGGGGT+TGG + chr5.4:22702025-22702044 MS.gene44265:CDS 65.0%
GGCGGCTGTTGCGTCGAATC+CGG + chr5.4:22702345-22702364 MS.gene44265:CDS 65.0%
GGCTGGACTTATTGCCGGCG+GGG + chr5.4:22702021-22702040 MS.gene44265:CDS 65.0%
GGCTGTTGCGTCGAATCCGG+TGG + chr5.4:22702348-22702367 MS.gene44265:CDS 65.0%
TAACCCCGCCGACGTTGCCA+TGG + chr5.4:22702060-22702079 MS.gene44265:CDS 65.0%
TCCGGTTCCTCAGCCTCGTG+TGG + chr5.4:22701811-22701830 MS.gene44265:CDS 65.0%
! GAGCACAGTGGCGGAGATGC+CGG - chr5.4:22701894-22701913 None:intergenic 65.0%
!! CGAAAGCAAGTGCCGGTCGG+AGG - chr5.4:22701754-22701773 None:intergenic 65.0%
!! GGCGGGGTTGGTGCTGCTAT+CGG + chr5.4:22702037-22702056 MS.gene44265:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 22701617 22702585 22701617 ID=MS.gene44265
chr5.4 mRNA 22701617 22702585 22701617 ID=MS.gene44265.t1;Parent=MS.gene44265
chr5.4 exon 22701617 22702585 22701617 ID=MS.gene44265.t1.exon1;Parent=MS.gene44265.t1
chr5.4 CDS 22701617 22702585 22701617 ID=cds.MS.gene44265.t1;Parent=MS.gene44265.t1
Gene Sequence

>MS.gene44265

ATGGGTGTCAAAGGTTTTGTTGAAGGTGGTATTGCTTCAATCATCGCAGGATGTTCCACACATCCACTAGATCTTATTAAGGTTCGCATGCAACTTCAAGGCGAAAACGCGCCTAAGCCGAACCCGGTTCAGATCCTCCGACCGGCACTTGCTTTCGGTCAAACCGGAACAACCACGATCCATGTGGGGTCTACTCCGGTTCCTCAGCCTCGTGTGGGCCTTATCTCAGTCGGAGTCCGTCTTGTTCAACAAGAAGGTGTAACAGCTTTGTTCTCCGGCATCTCCGCCACTGTGCTCAGGCAGACACTGTATTCCACCACCAGGATGGGACTCTATGAGGTTCTCAAAAACAAGTGGACAGATCGTGAAGCTGGCGGCACTATGCCGCTGTTTCGCAAGATCGAGGCTGGACTTATTGCCGGCGGGGTTGGTGCTGCTATCGGTAACCCCGCCGACGTTGCCATGGTTCGAATGCAAGCCGATGGGAGACTCCCACCGGCTCAGCAGAGAAACTACAAATCTGTTGTCGACGCCATTACTAGAATGGCGAAGCAGGAAGGTGTGACCAGTCTTTGGCGCGGGTCATCATTGACTGTGAACCGCGCCATGCTGGTCACAGCTTCACAGCTGGCATCATATGATCAGTTCAAGGAGATGATTCTTGAGAAGGGTGTTATGCGTGATGGGCTTGGGACCCATGTGACAGCTAGTTTTGCAGCGGGATTTGTGGCGGCTGTTGCGTCGAATCCGGTGGATGTTATTAAGACAAGGGTGATGAACATGAAGGTGGAGGTTGGAAAGGAACCACCTTATGCTGGTGCTTTGGATTGTGCTTTGAAGACAGTTCGTGCTGAGGGTCCTTTGGCTCTTTACAAGGGATTTATTCCTACAATTTCAAGGCAGGGACCTTTCACTGTTGTTCTGTTTGTTACTTTGGAGCAAGTTCGCAAGTTGTTGAAGGATTTCTGA
Protein sequence

>MS.gene44265.t1

MGVKGFVEGGIASIIAGCSTHPLDLIKVRMQLQGENAPKPNPVQILRPALAFGQTGTTTIHVGSTPVPQPRVGLISVGVRLVQQEGVTALFSGISATVLRQTLYSTTRMGLYEVLKNKWTDREAGGTMPLFRKIEAGLIAGGVGAAIGNPADVAMVRMQADGRLPPAQQRNYKSVVDAITRMAKQEGVTSLWRGSSLTVNRAMLVTASQLASYDQFKEMILEKGVMRDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEVGKEPPYAGALDCALKTVRAEGPLALYKGFIPTISRQGPFTVVLFVTLEQVRKLLKDF