Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44575.t1 | KEH17122.1 | 90.8 | 65 | 6 | 0 | 2 | 66 | 143 | 207 | 6.10E-27 | 129.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44575.t1 | A0A072TI67 | 90.8 | 65 | 6 | 0 | 2 | 66 | 143 | 207 | 4.4e-27 | 129.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049853 | MS.gene44575 | 0.833891 | 4.11E-56 | -1.69E-46 |
| MS.gene052447 | MS.gene44575 | 0.82463 | 7.27E-54 | -1.69E-46 |
| MS.gene054174 | MS.gene44575 | 0.806586 | 7.66E-50 | -1.69E-46 |
| MS.gene054175 | MS.gene44575 | 0.810491 | 1.12E-50 | -1.69E-46 |
| MS.gene054484 | MS.gene44575 | 0.813108 | 3.02E-51 | -1.69E-46 |
| MS.gene055058 | MS.gene44575 | 0.821939 | 3.09E-53 | -1.69E-46 |
| MS.gene055952 | MS.gene44575 | 0.85368 | 2.04E-61 | -1.69E-46 |
| MS.gene059276 | MS.gene44575 | 0.802754 | 4.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATGTTATTTTGATATTCTT+AGG | + | 43391:8579-8598 | MS.gene44575:intron | 20.0% |
| !! | TTTTGATATTCTTAGGTATG+AGG | + | 43391:8586-8605 | MS.gene44575:intron | 25.0% |
| GATAACATTAGCATTACCTA+GGG | - | 43391:8517-8536 | MS.gene44575:intergenic | 30.0% | |
| GCTAATGTTATCCTAATGAT+TGG | + | 43391:8525-8544 | MS.gene44575:intron | 30.0% | |
| ACACAAAGACACCAATCATT+AGG | - | 43391:8539-8558 | MS.gene44574:intergenic | 35.0% | |
| AGGTAAACAGTGTAGTGATT+TGG | + | 43391:8682-8701 | MS.gene44575:CDS | 35.0% | |
| GGATAACATTAGCATTACCT+AGG | - | 43391:8518-8537 | MS.gene44575:intergenic | 35.0% | |
| !! | GTTGTGTTTGTTGTGTTTGA+CGG | + | 43391:8641-8660 | MS.gene44575:CDS | 35.0% |
| TATGAGGTGAAGATCTGTGT+CGG | + | 43391:8602-8621 | MS.gene44575:CDS | 40.0% | |
| ! | GGTGATATGCAGAATTTGCT+AGG | + | 43391:8662-8681 | MS.gene44575:CDS | 40.0% |
| !! | ACAGTGTAGTGATTTGGTGT+CGG | + | 43391:8688-8707 | MS.gene44575:CDS | 40.0% |
| !! | TTTGGTGCTTACTACTGCAA+GGG | + | 43391:8464-8483 | MS.gene44575:CDS | 40.0% |
| ATGTGTTTCAGATGGTCCCT+AGG | + | 43391:8498-8517 | MS.gene44575:CDS | 45.0% | |
| CAGTAGTAAGCACCAAAGTC+GGG | - | 43391:8461-8480 | MS.gene44575:intergenic | 45.0% | |
| CGTGAAGCATGTGTTTCAGA+TGG | + | 43391:8490-8509 | MS.gene44575:CDS | 45.0% | |
| GCAGTAGTAAGCACCAAAGT+CGG | - | 43391:8462-8481 | MS.gene44575:intergenic | 45.0% | |
| !! | CTTTGGTGCTTACTACTGCA+AGG | + | 43391:8463-8482 | MS.gene44575:CDS | 45.0% |
| !! | AGAGGTGTTACACCCGACTT+TGG | + | 43391:8446-8465 | MS.gene44575:CDS | 50.0% |
| ! | ATCTGTGTCGGCGATGGTGA+TGG | + | 43391:8614-8633 | MS.gene44575:CDS | 55.0% |
| ! | GTGAAGATCTGTGTCGGCGA+TGG | + | 43391:8608-8627 | MS.gene44575:CDS | 55.0% |
| ! | TCTGTGTCGGCGATGGTGAT+GGG | + | 43391:8615-8634 | MS.gene44575:CDS | 55.0% |
| !! | TGATTTGGTGTCGGCTGCCA+AGG | + | 43391:8697-8716 | MS.gene44575:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43391 | gene | 8439 | 8718 | 8439 | ID=MS.gene44575 |
| 43391 | mRNA | 8439 | 8718 | 8439 | ID=MS.gene44575.t1;Parent=MS.gene44575 |
| 43391 | exon | 8439 | 8519 | 8439 | ID=MS.gene44575.t1.exon1;Parent=MS.gene44575.t1 |
| 43391 | CDS | 8439 | 8519 | 8439 | ID=cds.MS.gene44575.t1;Parent=MS.gene44575.t1 |
| 43391 | exon | 8601 | 8718 | 8601 | ID=MS.gene44575.t1.exon2;Parent=MS.gene44575.t1 |
| 43391 | CDS | 8601 | 8718 | 8601 | ID=cds.MS.gene44575.t1;Parent=MS.gene44575.t1 |
| Gene Sequence |
| Protein sequence |
