AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45522

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45522.t1 MTR_2g097467 95.122 656 32 0 1 656 1 656 0.0 1258
MS.gene45522.t1 MTR_2g097473 92.378 656 50 0 1 656 1 656 0.0 1239
MS.gene45522.t1 MTR_2g097463 82.192 657 116 1 1 656 1 657 0.0 1108
MS.gene45522.t1 MTR_4g064200 54.993 691 237 13 1 656 1 652 0.0 695
MS.gene45522.t1 MTR_7g062120 44.331 688 297 15 44 656 66 742 0.0 539
MS.gene45522.t1 MTR_4g064120 45.171 642 296 15 38 656 20 628 1.14e-171 504
MS.gene45522.t1 MTR_4g064150 43.917 674 306 19 38 656 79 735 1.17e-169 503
MS.gene45522.t1 MTR_4g064180 45.016 642 297 15 38 656 20 628 2.38e-168 495
MS.gene45522.t1 MTR_4g064160 52.665 469 203 6 200 656 225 686 4.83e-168 497
MS.gene45522.t1 MTR_2g097410 50.873 458 219 3 205 656 286 743 2.58e-165 491
MS.gene45522.t1 MTR_2g097310 38.699 615 341 12 51 656 53 640 5.65e-138 418
MS.gene45522.t1 MTR_2g097390 45.872 436 221 6 227 656 217 643 2.70e-134 409
MS.gene45522.t1 MTR_2g097350 42.767 477 260 6 185 656 174 642 1.48e-132 404
MS.gene45522.t1 MTR_2g097380 48.361 366 159 5 292 656 227 563 2.38e-120 370
MS.gene45522.t1 MTR_5g097480 32.283 381 252 5 277 653 165 543 1.59e-58 206
MS.gene45522.t1 MTR_2g026250 29.443 377 261 4 281 653 220 595 8.81e-57 202
MS.gene45522.t1 MTR_2g082090 32.225 391 259 5 267 653 190 578 1.19e-56 202
MS.gene45522.t1 MTR_4g133660 29.211 380 262 6 280 654 166 543 5.22e-54 194
MS.gene45522.t1 MTR_7g027190 29.592 392 244 5 282 653 293 672 4.20e-52 191
MS.gene45522.t1 MTR_3g056110 29.521 376 261 3 281 653 167 541 1.52e-51 187
MS.gene45522.t1 MTR_1g029420 28.533 368 259 3 289 653 225 591 9.21e-51 186
MS.gene45522.t1 MTR_4g104020 29.351 385 244 7 286 653 146 519 3.78e-50 182
MS.gene45522.t1 MTR_5g094450 30.688 378 250 8 285 653 157 531 5.92e-50 182
MS.gene45522.t1 MTR_8g442410 28.682 387 255 5 283 656 149 527 6.61e-48 177
MS.gene45522.t1 MTR_3g089055 27.717 368 262 3 289 653 198 564 1.15e-47 177
MS.gene45522.t1 MTR_3g089055 27.717 368 262 3 289 653 203 569 1.33e-47 176
MS.gene45522.t1 MTR_3g065980 29.668 391 238 9 283 653 166 539 1.38e-45 171
MS.gene45522.t1 MTR_7g074650 29.700 367 241 7 292 655 441 793 2.92e-43 167
MS.gene45522.t1 MTR_3g022830 27.578 417 242 7 290 653 26 435 3.09e-41 156
MS.gene45522.t1 MTR_2g034260 29.716 387 247 8 284 653 205 583 4.61e-40 155
MS.gene45522.t1 MTR_4g076140 25.814 430 251 5 286 653 45 468 2.26e-38 148
MS.gene45522.t1 MTR_2g034250 28.462 390 249 8 284 653 205 584 1.48e-37 148
MS.gene45522.t1 MTR_4g076020 27.926 376 229 9 292 656 87 431 4.56e-37 144
MS.gene45522.t1 MTR_4g102790 24.742 388 267 9 283 655 286 663 9.53e-37 146
MS.gene45522.t1 MTR_2g089100 27.363 402 250 11 289 656 52 445 8.29e-36 140
MS.gene45522.t1 MTR_4g077760 25.729 377 259 7 286 653 190 554 1.77e-33 135
MS.gene45522.t1 MTR_4g097080 24.668 377 269 7 289 655 133 504 5.67e-33 133
MS.gene45522.t1 MTR_1g069725 26.509 381 249 12 283 653 103 462 6.12e-32 130
MS.gene45522.t1 MTR_7g109580 26.442 416 264 10 253 653 165 553 7.75e-32 130
MS.gene45522.t1 MTR_1g096030 25.980 408 257 12 277 653 65 458 3.87e-31 127
MS.gene45522.t1 MTR_2g034280 26.632 383 262 8 284 653 198 574 7.54e-31 128
MS.gene45522.t1 MTR_5g015490 25.000 384 265 8 289 655 114 491 9.29e-31 126
MS.gene45522.t1 MTR_5g058860 25.978 358 235 10 316 653 149 496 8.62e-27 115
MS.gene45522.t1 MTR_7g057230 27.758 281 193 5 375 653 384 656 8.80e-27 116
MS.gene45522.t1 MTR_3g072710 27.346 373 226 14 308 653 145 499 6.72e-26 112
MS.gene45522.t1 MTR_6g047750 26.149 348 228 10 321 653 328 661 7.08e-25 110
MS.gene45522.t1 MTR_5g009080 31.100 209 134 2 289 493 56 258 4.46e-24 106
MS.gene45522.t1 MTR_5g009080 29.530 149 105 0 505 653 329 477 1.02e-16 84.0
MS.gene45522.t1 MTR_1g086970 23.729 354 243 8 316 653 135 477 1.14e-23 105
MS.gene45522.t1 MTR_5g015950 21.823 417 274 9 289 656 35 448 2.12e-23 103
MS.gene45522.t1 MTR_3g053270 21.823 417 274 9 289 656 35 448 2.12e-23 103
MS.gene45522.t1 MTR_4g095500 23.206 418 265 10 289 656 59 470 1.55e-22 101
MS.gene45522.t1 MTR_8g093070 23.747 379 249 16 294 656 144 498 2.95e-22 101
MS.gene45522.t1 MTR_2g099110 24.725 364 224 9 289 623 74 416 2.10e-21 98.2
MS.gene45522.t1 MTR_4g026485 22.137 393 272 11 275 653 251 623 2.12e-17 86.7
MS.gene45522.t1 MTR_0092s0100 23.450 371 261 9 290 653 373 727 3.75e-17 85.9
MS.gene45522.t1 MTR_0092s0100 23.450 371 261 9 290 653 376 730 3.93e-17 85.9
MS.gene45522.t1 MTR_8g020840 25.000 404 237 17 289 653 171 547 6.32e-16 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45522.t1 AT5G59450 42.997 614 265 10 54 656 61 600 1.01e-165 488
MS.gene45522.t1 AT2G29060 42.677 635 301 14 50 655 56 656 1.21e-165 489
MS.gene45522.t1 AT2G29060 42.481 645 325 11 50 655 56 693 4.71e-164 487
MS.gene45522.t1 AT2G37650 41.975 648 324 12 50 655 77 714 1.66e-160 478
MS.gene45522.t1 AT2G37650 41.975 648 324 12 50 655 65 702 1.96e-160 478
MS.gene45522.t1 AT1G07530 49.789 474 223 5 195 656 297 767 4.14e-159 476
MS.gene45522.t1 AT3G46600 51.448 449 195 6 216 656 148 581 3.15e-153 455
MS.gene45522.t1 AT3G46600 51.448 449 195 6 216 656 116 549 5.18e-153 453
MS.gene45522.t1 AT3G46600 56.216 370 160 1 289 656 4 373 3.47e-151 442
MS.gene45522.t1 AT2G29065 42.400 625 313 14 51 656 34 630 2.86e-148 444
MS.gene45522.t1 AT1G07520 46.058 482 242 8 185 656 58 531 2.27e-137 412
MS.gene45522.t1 AT1G07520 46.058 482 242 8 185 656 58 531 2.27e-137 412
MS.gene45522.t1 AT1G07520 46.058 482 242 8 185 656 223 696 7.60e-136 414
MS.gene45522.t1 AT1G07520 46.058 482 242 8 185 656 222 695 7.96e-136 414
MS.gene45522.t1 AT1G50600 33.251 403 261 6 256 653 126 525 4.34e-60 210
MS.gene45522.t1 AT1G50600 33.251 403 261 6 256 653 197 596 1.97e-59 210
MS.gene45522.t1 AT1G50600 33.251 403 261 6 256 653 201 600 2.65e-59 210
MS.gene45522.t1 AT5G48150 31.414 382 257 3 276 653 109 489 3.62e-58 204
MS.gene45522.t1 AT5G48150 31.414 382 257 3 276 653 109 489 3.62e-58 204
MS.gene45522.t1 AT5G48150 31.414 382 257 3 276 653 109 489 3.62e-58 204
MS.gene45522.t1 AT5G48150 31.414 382 257 3 276 653 109 489 3.62e-58 204
MS.gene45522.t1 AT2G04890 30.108 372 249 4 285 653 49 412 2.11e-51 183
MS.gene45522.t1 AT4G17230 29.867 375 259 3 284 655 153 526 1.09e-48 179
MS.gene45522.t1 AT4G17230 29.867 375 259 3 284 655 153 526 1.09e-48 179
MS.gene45522.t1 AT1G21450 27.717 368 263 2 289 653 225 592 8.19e-47 175
MS.gene45522.t1 AT1G14920 31.481 378 237 11 283 653 166 528 1.21e-46 173
MS.gene45522.t1 AT3G03450 30.077 389 233 9 283 653 177 544 7.55e-46 171
MS.gene45522.t1 AT1G66350 30.380 395 234 11 276 653 135 505 4.18e-45 168
MS.gene45522.t1 AT3G54220 30.295 373 234 9 292 655 296 651 6.04e-44 167
MS.gene45522.t1 AT2G01570 28.800 375 251 9 283 653 218 580 3.50e-43 164
MS.gene45522.t1 AT5G17490 30.233 387 229 12 283 653 154 515 5.70e-43 162
MS.gene45522.t1 AT5G66770 28.418 373 256 7 286 653 217 583 5.35e-42 161
MS.gene45522.t1 AT5G41920 27.013 385 253 7 279 653 33 399 2.51e-38 147
MS.gene45522.t1 AT1G50420 25.522 431 252 8 286 653 54 478 5.23e-38 147
MS.gene45522.t1 AT3G50650 25.867 375 261 7 290 653 173 541 1.56e-36 144
MS.gene45522.t1 AT4G37650 25.310 403 249 11 288 655 145 530 1.12e-34 139
MS.gene45522.t1 AT1G63100 25.765 392 259 9 285 654 248 629 9.97e-33 134
MS.gene45522.t1 AT1G63100 26.020 392 258 9 285 654 273 654 1.17e-32 134
MS.gene45522.t1 AT5G52510 27.083 384 243 11 292 653 271 639 5.12e-32 132
MS.gene45522.t1 AT3G49950 27.551 392 247 11 289 655 30 409 1.56e-30 124
MS.gene45522.t1 AT4G08250 27.473 364 237 13 305 653 125 476 4.04e-27 115
MS.gene45522.t1 AT1G55580 22.739 387 233 9 318 653 73 444 1.22e-21 99.0
MS.gene45522.t1 AT3G13840 23.514 387 249 14 289 653 148 509 3.24e-18 88.6
MS.gene45522.t1 AT4G00150 21.792 413 288 10 249 653 168 553 5.24e-14 75.9
MS.gene45522.t1 AT5G67411 29.703 202 125 9 402 590 1 198 6.79e-12 65.5

Find 138 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGACTACAAAATCTTTA+TGG 0.219672 2.1:+6541914 None:intergenic
CCATCTTCACCTTCTTCTTC+TGG 0.229698 2.1:-6543701 MS.gene45522:CDS
CTGGGTCAGTGCTACCATTT+GGG 0.242938 2.1:+6543492 None:intergenic
TATGTATCTTAGGATGTTAT+TGG 0.255000 2.1:+6543637 None:intergenic
AGCGTTCATGTTATTGATTT+TGG 0.271020 2.1:-6542621 MS.gene45522:CDS
CCTCCAAAGCTTCGAATTAC+AGG 0.281535 2.1:-6542537 MS.gene45522:CDS
GTTACTTGGTTTACTGAGTT+TGG 0.288731 2.1:+6543749 None:intergenic
AGGCAACACTCTTCGCCTTT+TGG 0.305061 2.1:-6542852 MS.gene45522:CDS
ACTCCATGGAAGAAGACTTT+TGG 0.314241 2.1:+6542243 None:intergenic
CAAAATTATAAGATGTTTGT+TGG 0.315251 2.1:+6543033 None:intergenic
TTCCTTCTTCACCATTGCTT+TGG 0.317965 2.1:+6541939 None:intergenic
GCAGGAATGTTTAAGACTTC+AGG 0.330542 2.1:-6543561 MS.gene45522:CDS
TTTGTATGCTTTATCTGCTT+GGG 0.337273 2.1:-6541851 MS.gene45522:CDS
TCTGGGTCAGTGCTACCATT+TGG 0.342818 2.1:+6543491 None:intergenic
ATAACTCATATGAATCTAAC+TGG 0.344405 2.1:-6543412 MS.gene45522:CDS
AAGTCCTTCTATGATGTTCT+TGG 0.346747 2.1:-6543530 MS.gene45522:CDS
ACAACTTCATTGCACAGAAA+TGG 0.347339 2.1:-6542413 MS.gene45522:CDS
TTAGCTCATTACTTTGCTAA+TGG 0.355828 2.1:-6542813 MS.gene45522:CDS
GGATGTCACTTATGTATCTT+AGG 0.358360 2.1:+6543627 None:intergenic
ATTCCTGTAATTCGAAGCTT+TGG 0.359837 2.1:+6542534 None:intergenic
AATCAGGTTGGATTCTGATT+TGG 0.361148 2.1:-6541971 MS.gene45522:CDS
TAAAGGAGTTTGTAGTTGTA+TGG 0.363980 2.1:+6543358 None:intergenic
GCAGAAAGGGTGGAGGAAAC+TGG 0.374425 2.1:-6542483 MS.gene45522:CDS
AAATCTTGCAGAAAGGTGTT+TGG 0.378133 2.1:+6543779 None:intergenic
ATCGCTGTTTGATATGTTTG+AGG 0.385635 2.1:-6542154 MS.gene45522:CDS
CCTCTTGGTTTGGCAACTGC+TGG 0.385884 2.1:-6542750 MS.gene45522:CDS
TCTTCTGGCACAAGCTCAAA+TGG 0.388620 2.1:-6543686 MS.gene45522:CDS
AGCTTCAACACAGCTTCTCT+TGG 0.390505 2.1:+6542282 None:intergenic
ATCATATCAGCCTCTTGGTT+TGG 0.393034 2.1:-6542760 MS.gene45522:CDS
GTTTCCTCCACCCTTTCTGC+AGG 0.394328 2.1:+6542486 None:intergenic
AAGCTGTGTTGAAGCTGATC+AGG 0.396558 2.1:-6542275 MS.gene45522:CDS
TCAGTAAACCAAGTAACAAA+TGG 0.401437 2.1:-6543743 MS.gene45522:CDS
CCTCGTGTCTCGATGACAAC+TGG 0.405616 2.1:+6543299 None:intergenic
ATTCAAACCCATTTGTTACT+TGG 0.405951 2.1:+6543735 None:intergenic
ACCATTGTCACAACGGAACT+TGG 0.411323 2.1:-6543271 MS.gene45522:CDS
GAGCTGGGTTCAAGCAAATC+AGG 0.413441 2.1:-6541987 MS.gene45522:CDS
TTCTGACGAGGCACGGCCAC+TGG 0.426845 2.1:+6542583 None:intergenic
CAACTTCATTGCACAGAAAT+GGG 0.427023 2.1:-6542412 MS.gene45522:CDS
CGTACAAGCAGTGGCAGGTC+AGG 0.432935 2.1:-6542017 MS.gene45522:CDS
ATGCTTGAGAGAGGGTTGTT+TGG 0.436357 2.1:-6542099 MS.gene45522:CDS
ATGTTATTGGAATATCTTGT+CGG 0.439317 2.1:+6543650 None:intergenic
TTCAATGCCACAGTTTCATC+AGG 0.440053 2.1:+6542309 None:intergenic
GCACGATCGAAATTTCCATC+TGG 0.452751 2.1:+6543473 None:intergenic
TGCCACTGCTTGTACGTCTC+AGG 0.458186 2.1:+6542024 None:intergenic
CATTTGGGGATGGATGGTTA+TGG 0.459747 2.1:+6543507 None:intergenic
AAGCTCAAATGGAGAATCCT+TGG 0.463718 2.1:-6543675 MS.gene45522:CDS
CATGGATGAAGAAGATGACT+TGG 0.465816 2.1:-6543603 MS.gene45522:CDS
TTGAGAGAGGGTTGTTTGGA+AGG 0.472118 2.1:-6542095 MS.gene45522:CDS
ATGTTGCGGCTGTTGATTTG+TGG 0.473041 2.1:-6542950 MS.gene45522:CDS
TAAACATTCCTGCAACATGC+AGG 0.474161 2.1:+6543571 None:intergenic
GAAACTTCTTCCACCGTCAC+TGG 0.475504 2.1:+6543230 None:intergenic
TGCTACCATTTGGGGATGGA+TGG 0.476955 2.1:+6543501 None:intergenic
GTGCTTCCTTAAACCGAGTC+AGG 0.477530 2.1:+6542190 None:intergenic
AGTACCTCATCTAAAATCTC+TGG 0.483079 2.1:+6543089 None:intergenic
AGCTTGTGAGGGAGCAGAAA+GGG 0.488617 2.1:-6542055 MS.gene45522:CDS
CTTTCTGCAGGTCTAAATCC+TGG 0.490039 2.1:+6542498 None:intergenic
TAGTCGATGTGGATGGTAAG+TGG 0.492425 2.1:-6541900 MS.gene45522:CDS
CGAAGCTTTGGAGGACCACC+AGG 0.497005 2.1:+6542546 None:intergenic
CCTGTAATTCGAAGCTTTGG+AGG 0.497546 2.1:+6542537 None:intergenic
AACCAAAGCAATGGTGAAGA+AGG 0.500696 2.1:-6541941 MS.gene45522:CDS
ATGGCCAAGAACATCATAGA+AGG 0.501527 2.1:+6543526 None:intergenic
AATCCAAAAGTCTTCTTCCA+TGG 0.502095 2.1:-6542246 MS.gene45522:CDS
TAATGGTCTTGAGATAAGAT+TGG 0.503318 2.1:-6542796 MS.gene45522:CDS
GGAATTGAGCTTCCTCAGCC+AGG 0.503697 2.1:-6542516 MS.gene45522:CDS
CAGGTCAGGAACAAGAGAGC+TGG 0.506156 2.1:-6542003 MS.gene45522:CDS
CTTGAGATCCTTGAGACGGA+TGG 0.508334 2.1:+6542386 None:intergenic
TTCTTCCATGGAGTTGCCAA+TGG 0.510258 2.1:-6542234 MS.gene45522:CDS
ATGAGCTACTCAAACAGATT+AGG 0.511922 2.1:-6542872 MS.gene45522:CDS
AGGATTTAGACCTGCAGAAA+GGG 0.512549 2.1:-6542496 MS.gene45522:CDS
CAGTAAACCAAGTAACAAAT+GGG 0.514266 2.1:-6543742 MS.gene45522:CDS
AAACCAAGAGGCTGATATGA+TGG 0.515320 2.1:+6542762 None:intergenic
ACGCCATCATATCAGCCTCT+TGG 0.518385 2.1:-6542765 MS.gene45522:CDS
CACGATCGAAATTTCCATCT+GGG 0.518624 2.1:+6543474 None:intergenic
CGTTGGAGTCCATTGCCAAA+AGG 0.523700 2.1:+6542837 None:intergenic
GGAATATCTTGTCGGTTCCA+AGG 0.526658 2.1:+6543658 None:intergenic
GAGCTTGTGCCAGAAGAAGA+AGG 0.528577 2.1:+6543692 None:intergenic
CTGTGGCGAGCTACGATCAA+AGG 0.536487 2.1:-6542902 MS.gene45522:CDS
TCCAAGTTCCGTTGTGACAA+TGG 0.537164 2.1:+6543270 None:intergenic
TGAGAGAGGGTTGTTTGGAA+GGG 0.539295 2.1:-6542094 MS.gene45522:CDS
AACTTCCATTGGCAACTCCA+TGG 0.540581 2.1:+6542229 None:intergenic
CATAACCATCCATCCCCAAA+TGG 0.544430 2.1:-6543506 MS.gene45522:CDS
GTCACAACGGAACTTGGAGT+TGG 0.544454 2.1:-6543265 MS.gene45522:CDS
TATAAATGTTATAGCTTGTG+AGG 0.544729 2.1:-6542067 MS.gene45522:CDS
CAGGATTTAGACCTGCAGAA+AGG 0.549603 2.1:-6542497 MS.gene45522:CDS
GCAATGAAGTTGTATTCAAA+TGG 0.550757 2.1:+6542423 None:intergenic
AGTCGATGTGGATGGTAAGT+GGG 0.556259 2.1:-6541899 MS.gene45522:CDS
CGGTGCGCTATAACTTCCAT+TGG 0.556645 2.1:+6542218 None:intergenic
AACACTAAAATCTTGCAGAA+AGG 0.557457 2.1:+6543772 None:intergenic
TTGTGCACATTGATTTAGCA+TGG 0.558170 2.1:+6542926 None:intergenic
GCATTTGTCATTCTCTGCAA+AGG 0.559014 2.1:+6542687 None:intergenic
ATGCCACAGTTTCATCAGGA+AGG 0.560068 2.1:+6542313 None:intergenic
AGTAATGAACAAGAGGGAAG+AGG 0.563357 2.1:-6543152 MS.gene45522:CDS
ATGGGAAACCATCCGTCTCA+AGG 0.568717 2.1:-6542394 MS.gene45522:CDS
TCAGTGCTACCATTTGGGGA+TGG 0.569199 2.1:+6543497 None:intergenic
CAAAGTAATGAGCTAAACGT+TGG 0.573182 2.1:+6542820 None:intergenic
AGGTCAGGAACAAGAGAGCT+GGG 0.576170 2.1:-6542002 MS.gene45522:CDS
GAGGTACTGCTTTATCAGAC+AGG 0.576734 2.1:-6543074 MS.gene45522:CDS
AAACATTCCTGCAACATGCA+GGG 0.578865 2.1:+6543572 None:intergenic
ATACATAAGTGACATCCTCA+TGG 0.582153 2.1:-6543621 MS.gene45522:CDS
AGGGAGCAGAAAGGGTTGAA+AGG 0.587349 2.1:-6542047 MS.gene45522:CDS
TATCAAACAGCGATGAGAAG+TGG 0.592685 2.1:+6542163 None:intergenic
CCAGAAGAAGAAGGTGAAGA+TGG 0.592856 2.1:+6543701 None:intergenic
TGATACTAGTAATGAACAAG+AGG 0.593763 2.1:-6543159 MS.gene45522:CDS
TGAGACGTACAAGCAGTGGC+AGG 0.594436 2.1:-6542022 MS.gene45522:CDS
CAGAGGTCGATGCTTGAGAG+AGG 0.600053 2.1:-6542108 MS.gene45522:CDS
TGATTCAGGAGGAAAGGCAA+AGG 0.600358 2.1:-6543012 MS.gene45522:CDS
ATTTAGACCTGCAGAAAGGG+TGG 0.601556 2.1:-6542493 MS.gene45522:CDS
ACCTCATCTAAAATCTCTGG+TGG 0.604084 2.1:+6543092 None:intergenic
TGGGTCAGTGCTACCATTTG+GGG 0.604759 2.1:+6543493 None:intergenic
AATGGTAGCACTGACCCAGA+TGG 0.606409 2.1:-6543488 MS.gene45522:CDS
ATAAATGTTATAGCTTGTGA+GGG 0.613447 2.1:-6542066 MS.gene45522:CDS
TGGGTTCAAGCAAATCAGGT+TGG 0.613823 2.1:-6541983 MS.gene45522:CDS
ACAAGAGGGAAGAGGTAGCA+AGG 0.614187 2.1:-6543144 MS.gene45522:CDS
GCAGGTCTAAATCCTGGCTG+AGG 0.615245 2.1:+6542504 None:intergenic
GGCCTGAGACGTACAAGCAG+TGG 0.615272 2.1:-6542026 MS.gene45522:CDS
TAGACCTGCAGAAAGGGTGG+AGG 0.621359 2.1:-6542490 MS.gene45522:CDS
ATCTACTACGAACAATGTTG+CGG 0.623727 2.1:-6542964 MS.gene45522:CDS
TAGCTTGTGAGGGAGCAGAA+AGG 0.625836 2.1:-6542056 MS.gene45522:CDS
CTATCTTGAGATCCTTGAGA+CGG 0.633125 2.1:+6542382 None:intergenic
GATACTAGTAATGAACAAGA+GGG 0.634217 2.1:-6543158 MS.gene45522:CDS
TGACATTCAGCCAGTGACGG+TGG 0.640113 2.1:-6543240 MS.gene45522:CDS
CCAGCAGTTGCCAAACCAAG+AGG 0.642152 2.1:+6542750 None:intergenic
GACCTCTGCGTGTCTTCACG+AGG 0.643181 2.1:+6542123 None:intergenic
AGAAAACTTTCATTAAGACC+TGG 0.645902 2.1:-6542564 MS.gene45522:CDS
AAGAGTCGAATTCGACAACA+TGG 0.647441 2.1:+6543384 None:intergenic
GAATGACATTCAGCCAGTGA+CGG 0.650343 2.1:-6543243 MS.gene45522:CDS
AGAGGTCGATGCTTGAGAGA+GGG 0.653291 2.1:-6542107 MS.gene45522:CDS
CCAGTTGTCATCGAGACACG+AGG 0.656087 2.1:-6543299 MS.gene45522:CDS
GGTGAATGAAACCAAAGCAA+TGG 0.658724 2.1:-6541950 MS.gene45522:CDS
GAACCTTCCTGATGAAACTG+TGG 0.660816 2.1:-6542316 MS.gene45522:CDS
AAATCAATGTGCACAAGCTG+TGG 0.661819 2.1:-6542919 MS.gene45522:CDS
AAAGGGTGGAGGAAACTGGA+CGG 0.662270 2.1:-6542479 MS.gene45522:CDS
AAACTTTCATTAAGACCTGG+TGG 0.664266 2.1:-6542561 MS.gene45522:CDS
AGATTCATATGAGTTATCAG+TGG 0.666994 2.1:+6543418 None:intergenic
TAACTCATATGAATCTAACT+GGG 0.675488 2.1:-6543411 MS.gene45522:CDS
AGTCATCTTCTTCATCCATG+AGG 0.688746 2.1:+6543606 None:intergenic
GGTGCTAACCATTGTCACAA+CGG 0.691376 2.1:-6543278 MS.gene45522:CDS
TGCCTCGTGAAGACACGCAG+AGG 0.691967 2.1:-6542125 MS.gene45522:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTAGTTTTTTTCTATTAA+AGG + chr2.1:6542290-6542309 None:intergenic 15.0%
!! CAAAATTATAAGATGTTTGT+TGG + chr2.1:6542598-6542617 None:intergenic 20.0%
!!! ACATCTTATAATTTTGATTC+AGG - chr2.1:6542602-6542621 MS.gene45522:CDS 20.0%
!!! ATTGATTTTGGAATTTTCTA+TGG - chr2.1:6543019-6543038 MS.gene45522:CDS 20.0%
! AGAAAAAAGAAGTCATAAGA+TGG - chr2.1:6542436-6542455 MS.gene45522:CDS 25.0%
! ATAACTCATATGAATCTAAC+TGG - chr2.1:6542216-6542235 MS.gene45522:CDS 25.0%
! ATGTTATTGGAATATCTTGT+CGG + chr2.1:6541981-6542000 None:intergenic 25.0%
! TAACTCATATGAATCTAACT+GGG - chr2.1:6542217-6542236 MS.gene45522:CDS 25.0%
! TATGTATCTTAGGATGTTAT+TGG + chr2.1:6541994-6542013 None:intergenic 25.0%
!! ATAAATGTTATAGCTTGTGA+GGG - chr2.1:6543562-6543581 MS.gene45522:CDS 25.0%
!! TATAAATGTTATAGCTTGTG+AGG - chr2.1:6543561-6543580 MS.gene45522:CDS 25.0%
!!! ATCTTATGACTTCTTTTTTC+TGG + chr2.1:6542437-6542456 None:intergenic 25.0%
!!! CACTATTTTGAAAATCGTTT+TGG + chr2.1:6542975-6542994 None:intergenic 25.0%
!!! TCTTATAATTTTGATTCAGG+AGG - chr2.1:6542605-6542624 MS.gene45522:CDS 25.0%
AACACTAAAATCTTGCAGAA+AGG + chr2.1:6541859-6541878 None:intergenic 30.0%
AGAAAACTTTCATTAAGACC+TGG - chr2.1:6543064-6543083 MS.gene45522:CDS 30.0%
AGATTCATATGAGTTATCAG+TGG + chr2.1:6542213-6542232 None:intergenic 30.0%
CAGTAAACCAAGTAACAAAT+GGG - chr2.1:6541886-6541905 MS.gene45522:CDS 30.0%
GATACTAGTAATGAACAAGA+GGG - chr2.1:6542470-6542489 MS.gene45522:CDS 30.0%
GCAATGAAGTTGTATTCAAA+TGG + chr2.1:6543208-6543227 None:intergenic 30.0%
TAAAGGAGTTTGTAGTTGTA+TGG + chr2.1:6542273-6542292 None:intergenic 30.0%
TCAGTAAACCAAGTAACAAA+TGG - chr2.1:6541885-6541904 MS.gene45522:CDS 30.0%
TGATACTAGTAATGAACAAG+AGG - chr2.1:6542469-6542488 MS.gene45522:CDS 30.0%
TTAGCTCATTACTTTGCTAA+TGG - chr2.1:6542815-6542834 MS.gene45522:CDS 30.0%
TTTGTATGCTTTATCTGCTT+GGG - chr2.1:6543777-6543796 MS.gene45522:CDS 30.0%
! ATTCAAACCCATTTGTTACT+TGG + chr2.1:6541896-6541915 None:intergenic 30.0%
! CATCGACTACAAAATCTTTA+TGG + chr2.1:6543717-6543736 None:intergenic 30.0%
! TAAAGATTTTGTAGTCGATG+TGG - chr2.1:6543717-6543736 MS.gene45522:CDS 30.0%
! TAATGGTCTTGAGATAAGAT+TGG - chr2.1:6542832-6542851 MS.gene45522:CDS 30.0%
! TTAATGAAAGTTTTCTGACG+AGG + chr2.1:6543060-6543079 None:intergenic 30.0%
! TTTTGTATGCTTTATCTGCT+TGG - chr2.1:6543776-6543795 MS.gene45522:CDS 30.0%
!!! AGCGTTCATGTTATTGATTT+TGG - chr2.1:6543007-6543026 MS.gene45522:CDS 30.0%
!!! TAATTTTGATTCAGGAGGAA+AGG - chr2.1:6542610-6542629 MS.gene45522:CDS 30.0%
AAACTTTCATTAAGACCTGG+TGG - chr2.1:6543067-6543086 MS.gene45522:CDS 35.0%
AAGTCCTTCTATGATGTTCT+TGG - chr2.1:6542098-6542117 MS.gene45522:CDS 35.0%
AATCCAAAAGTCTTCTTCCA+TGG - chr2.1:6543382-6543401 MS.gene45522:CDS 35.0%
ACAACTTCATTGCACAGAAA+TGG - chr2.1:6543215-6543234 MS.gene45522:CDS 35.0%
ATACATAAGTGACATCCTCA+TGG - chr2.1:6542007-6542026 MS.gene45522:CDS 35.0%
ATCGCTGTTTGATATGTTTG+AGG - chr2.1:6543474-6543493 MS.gene45522:CDS 35.0%
ATCTACTACGAACAATGTTG+CGG - chr2.1:6542664-6542683 MS.gene45522:CDS 35.0%
ATGAGCTACTCAAACAGATT+AGG - chr2.1:6542756-6542775 MS.gene45522:CDS 35.0%
ATTCCTGTAATTCGAAGCTT+TGG + chr2.1:6543097-6543116 None:intergenic 35.0%
CAAAGTAATGAGCTAAACGT+TGG + chr2.1:6542811-6542830 None:intergenic 35.0%
CAACTTCATTGCACAGAAAT+GGG - chr2.1:6543216-6543235 MS.gene45522:CDS 35.0%
GTTACTTGGTTTACTGAGTT+TGG + chr2.1:6541882-6541901 None:intergenic 35.0%
TTAAACCGAGTCAGGAAAAA+CGG + chr2.1:6543433-6543452 None:intergenic 35.0%
! AGTACCTCATCTAAAATCTC+TGG + chr2.1:6542542-6542561 None:intergenic 35.0%
! GAATTTTCTATGGTTTCCAG+TGG - chr2.1:6543029-6543048 MS.gene45522:CDS 35.0%
! GGATGTCACTTATGTATCTT+AGG + chr2.1:6542004-6542023 None:intergenic 35.0%
!! AAATCTTGCAGAAAGGTGTT+TGG + chr2.1:6541852-6541871 None:intergenic 35.0%
!! AATCAGGTTGGATTCTGATT+TGG - chr2.1:6543657-6543676 MS.gene45522:CDS 35.0%
!! TTGTGCACATTGATTTAGCA+TGG + chr2.1:6542705-6542724 None:intergenic 35.0%
!!! CTTTTTTCTGGTACTGTTGA+AGG + chr2.1:6542425-6542444 None:intergenic 35.0%
!!! GATGGTAAGTGGGTTTTAAA+GGG - chr2.1:6543739-6543758 MS.gene45522:CDS 35.0%
AAACATTCCTGCAACATGCA+GGG + chr2.1:6542059-6542078 None:intergenic 40.0%
AAACCAAGAGGCTGATATGA+TGG + chr2.1:6542869-6542888 None:intergenic 40.0%
AAATCAATGTGCACAAGCTG+TGG - chr2.1:6542709-6542728 MS.gene45522:CDS 40.0%
AACCAAAGCAATGGTGAAGA+AGG - chr2.1:6543687-6543706 MS.gene45522:CDS 40.0%
AAGAGTCGAATTCGACAACA+TGG + chr2.1:6542247-6542266 None:intergenic 40.0%
AAGCTCAAATGGAGAATCCT+TGG - chr2.1:6541953-6541972 MS.gene45522:CDS 40.0%
AGGATTTAGACCTGCAGAAA+GGG - chr2.1:6543132-6543151 MS.gene45522:CDS 40.0%
AGTAATGAACAAGAGGGAAG+AGG - chr2.1:6542476-6542495 MS.gene45522:CDS 40.0%
AGTCATCTTCTTCATCCATG+AGG + chr2.1:6542025-6542044 None:intergenic 40.0%
ATCATATCAGCCTCTTGGTT+TGG - chr2.1:6542868-6542887 MS.gene45522:CDS 40.0%
ATGGCCAAGAACATCATAGA+AGG + chr2.1:6542105-6542124 None:intergenic 40.0%
CACGATCGAAATTTCCATCT+GGG + chr2.1:6542157-6542176 None:intergenic 40.0%
CATGGATGAAGAAGATGACT+TGG - chr2.1:6542025-6542044 MS.gene45522:CDS 40.0%
CTATCTTGAGATCCTTGAGA+CGG + chr2.1:6543249-6543268 None:intergenic 40.0%
GCATTTGTCATTCTCTGCAA+AGG + chr2.1:6542944-6542963 None:intergenic 40.0%
GGTGAATGAAACCAAAGCAA+TGG - chr2.1:6543678-6543697 MS.gene45522:CDS 40.0%
TAAACATTCCTGCAACATGC+AGG + chr2.1:6542060-6542079 None:intergenic 40.0%
TATCAAACAGCGATGAGAAG+TGG + chr2.1:6543468-6543487 None:intergenic 40.0%
TTCAATGCCACAGTTTCATC+AGG + chr2.1:6543322-6543341 None:intergenic 40.0%
TTCCTTCTTCACCATTGCTT+TGG + chr2.1:6543692-6543711 None:intergenic 40.0%
! ACCTCATCTAAAATCTCTGG+TGG + chr2.1:6542539-6542558 None:intergenic 40.0%
! ACTCCATGGAAGAAGACTTT+TGG + chr2.1:6543388-6543407 None:intergenic 40.0%
! GATTTTGTAGTCGATGTGGA+TGG - chr2.1:6543721-6543740 MS.gene45522:CDS 40.0%
! GCAGGAATGTTTAAGACTTC+AGG - chr2.1:6542067-6542086 MS.gene45522:CDS 40.0%
!! GGATGGTAAGTGGGTTTTAA+AGG - chr2.1:6543738-6543757 MS.gene45522:CDS 40.0%
!!! GCTGCTAGTTTTGAGAGAAA+TGG - chr2.1:6542176-6542195 MS.gene45522:CDS 40.0%
!!! GTAAGTGGGTTTTAAAGGGA+TGG - chr2.1:6543743-6543762 MS.gene45522:CDS 40.0%
!!! GTTTTTCCTGACTCGGTTTA+AGG - chr2.1:6543432-6543451 MS.gene45522:CDS 40.0%
!!! TGGGTTTTAAAGGGATGGAA+AGG - chr2.1:6543748-6543767 MS.gene45522:CDS 40.0%
AACTTCCATTGGCAACTCCA+TGG + chr2.1:6543402-6543421 None:intergenic 45.0%
AAGCTGTGTTGAAGCTGATC+AGG - chr2.1:6543353-6543372 MS.gene45522:CDS 45.0%
ACCATTGTCACAACGGAACT+TGG - chr2.1:6542357-6542376 MS.gene45522:CDS 45.0%
AGCTTCAACACAGCTTCTCT+TGG + chr2.1:6543349-6543368 None:intergenic 45.0%
AGTCGATGTGGATGGTAAGT+GGG - chr2.1:6543729-6543748 MS.gene45522:CDS 45.0%
ATGCCACAGTTTCATCAGGA+AGG + chr2.1:6543318-6543337 None:intergenic 45.0%
ATGCTTGAGAGAGGGTTGTT+TGG - chr2.1:6543529-6543548 MS.gene45522:CDS 45.0%
ATTTAGACCTGCAGAAAGGG+TGG - chr2.1:6543135-6543154 MS.gene45522:CDS 45.0%
CAGGATTTAGACCTGCAGAA+AGG - chr2.1:6543131-6543150 MS.gene45522:CDS 45.0%
CATAACCATCCATCCCCAAA+TGG - chr2.1:6542122-6542141 MS.gene45522:CDS 45.0%
CATTTGGGGATGGATGGTTA+TGG + chr2.1:6542124-6542143 None:intergenic 45.0%
CCAGAAGAAGAAGGTGAAGA+TGG + chr2.1:6541930-6541949 None:intergenic 45.0%
CCATCTTCACCTTCTTCTTC+TGG - chr2.1:6541927-6541946 MS.gene45522:CDS 45.0%
CCTCCAAAGCTTCGAATTAC+AGG - chr2.1:6543091-6543110 MS.gene45522:CDS 45.0%
CTTTCTGCAGGTCTAAATCC+TGG + chr2.1:6543133-6543152 None:intergenic 45.0%
GAACCTTCCTGATGAAACTG+TGG - chr2.1:6543312-6543331 MS.gene45522:CDS 45.0%
GAATGACATTCAGCCAGTGA+CGG - chr2.1:6542385-6542404 MS.gene45522:CDS 45.0%
GCACGATCGAAATTTCCATC+TGG + chr2.1:6542158-6542177 None:intergenic 45.0%
GGTGCTAACCATTGTCACAA+CGG - chr2.1:6542350-6542369 MS.gene45522:CDS 45.0%
TAGTCGATGTGGATGGTAAG+TGG - chr2.1:6543728-6543747 MS.gene45522:CDS 45.0%
TCCAAGTTCCGTTGTGACAA+TGG + chr2.1:6542361-6542380 None:intergenic 45.0%
TCTTCTGGCACAAGCTCAAA+TGG - chr2.1:6541942-6541961 MS.gene45522:CDS 45.0%
TGAGAGAGGGTTGTTTGGAA+GGG - chr2.1:6543534-6543553 MS.gene45522:CDS 45.0%
TGATTCAGGAGGAAAGGCAA+AGG - chr2.1:6542616-6542635 MS.gene45522:CDS 45.0%
TGGGTTCAAGCAAATCAGGT+TGG - chr2.1:6543645-6543664 MS.gene45522:CDS 45.0%
TTCTTCCATGGAGTTGCCAA+TGG - chr2.1:6543394-6543413 MS.gene45522:CDS 45.0%
TTGAGAGAGGGTTGTTTGGA+AGG - chr2.1:6543533-6543552 MS.gene45522:CDS 45.0%
! CCTGTAATTCGAAGCTTTGG+AGG + chr2.1:6543094-6543113 None:intergenic 45.0%
! GAAAGTTTTCTGACGAGGCA+CGG + chr2.1:6543055-6543074 None:intergenic 45.0%
! GCCACCAGAGATTTTAGATG+AGG - chr2.1:6542535-6542554 MS.gene45522:CDS 45.0%
!! ATGTTGCGGCTGTTGATTTG+TGG - chr2.1:6542678-6542697 MS.gene45522:CDS 45.0%
!! GAGGTACTGCTTTATCAGAC+AGG - chr2.1:6542554-6542573 MS.gene45522:CDS 45.0%
!! GGAATATCTTGTCGGTTCCA+AGG + chr2.1:6541973-6541992 None:intergenic 45.0%
AAAGGGTGGAGGAAACTGGA+CGG - chr2.1:6543149-6543168 MS.gene45522:CDS 50.0%
ACAAGAGGGAAGAGGTAGCA+AGG - chr2.1:6542484-6542503 MS.gene45522:CDS 50.0%
ACGCCATCATATCAGCCTCT+TGG - chr2.1:6542863-6542882 MS.gene45522:CDS 50.0%
AGAGGTCGATGCTTGAGAGA+GGG - chr2.1:6543521-6543540 MS.gene45522:CDS 50.0%
AGCTTGTGAGGGAGCAGAAA+GGG - chr2.1:6543573-6543592 MS.gene45522:CDS 50.0%
AGGGAGCAGAAAGGGTTGAA+AGG - chr2.1:6543581-6543600 MS.gene45522:CDS 50.0%
AGGTCAGGAACAAGAGAGCT+GGG - chr2.1:6543626-6543645 MS.gene45522:CDS 50.0%
ATGGGAAACCATCCGTCTCA+AGG - chr2.1:6543234-6543253 MS.gene45522:CDS 50.0%
CGGTGCGCTATAACTTCCAT+TGG + chr2.1:6543413-6543432 None:intergenic 50.0%
CGTTGGAGTCCATTGCCAAA+AGG + chr2.1:6542794-6542813 None:intergenic 50.0%
CTGGGTCAGTGCTACCATTT+GGG + chr2.1:6542139-6542158 None:intergenic 50.0%
CTTGAGATCCTTGAGACGGA+TGG + chr2.1:6543245-6543264 None:intergenic 50.0%
GAAACTTCTTCCACCGTCAC+TGG + chr2.1:6542401-6542420 None:intergenic 50.0%
GAGCTGGGTTCAAGCAAATC+AGG - chr2.1:6543641-6543660 MS.gene45522:CDS 50.0%
GAGCTTGTGCCAGAAGAAGA+AGG + chr2.1:6541939-6541958 None:intergenic 50.0%
GCAAAAACCCTGCATGTTGC+AGG - chr2.1:6542049-6542068 MS.gene45522:CDS 50.0%
GTCACAACGGAACTTGGAGT+TGG - chr2.1:6542363-6542382 MS.gene45522:CDS 50.0%
GTGCTTCCTTAAACCGAGTC+AGG + chr2.1:6543441-6543460 None:intergenic 50.0%
TCAGTGCTACCATTTGGGGA+TGG + chr2.1:6542134-6542153 None:intergenic 50.0%
TCTGGGTCAGTGCTACCATT+TGG + chr2.1:6542140-6542159 None:intergenic 50.0%
TGCTACCATTTGGGGATGGA+TGG + chr2.1:6542130-6542149 None:intergenic 50.0%
TGGGTCAGTGCTACCATTTG+GGG + chr2.1:6542138-6542157 None:intergenic 50.0%
! AGGCAACACTCTTCGCCTTT+TGG - chr2.1:6542776-6542795 MS.gene45522:CDS 50.0%
! CACTCTTCGCCTTTTGGCAA+TGG - chr2.1:6542782-6542801 MS.gene45522:CDS 50.0%
! TAGCTTGTGAGGGAGCAGAA+AGG - chr2.1:6543572-6543591 MS.gene45522:CDS 50.0%
!! AATGGTAGCACTGACCCAGA+TGG - chr2.1:6542140-6542159 MS.gene45522:CDS 50.0%
CAGAGGTCGATGCTTGAGAG+AGG - chr2.1:6543520-6543539 MS.gene45522:CDS 55.0%
CAGGTCAGGAACAAGAGAGC+TGG - chr2.1:6543625-6543644 MS.gene45522:CDS 55.0%
CCAGCAGTTGCCAAACCAAG+AGG + chr2.1:6542881-6542900 None:intergenic 55.0%
CCAGTTGTCATCGAGACACG+AGG - chr2.1:6542329-6542348 MS.gene45522:CDS 55.0%
CCTCGTGTCTCGATGACAAC+TGG + chr2.1:6542332-6542351 None:intergenic 55.0%
CTGTGGCGAGCTACGATCAA+AGG - chr2.1:6542726-6542745 MS.gene45522:CDS 55.0%
GCAGAAAGGGTGGAGGAAAC+TGG - chr2.1:6543145-6543164 MS.gene45522:CDS 55.0%
GCAGGTCTAAATCCTGGCTG+AGG + chr2.1:6543127-6543146 None:intergenic 55.0%
GGAATTGAGCTTCCTCAGCC+AGG - chr2.1:6543112-6543131 MS.gene45522:CDS 55.0%
GTTTCCTCCACCCTTTCTGC+AGG + chr2.1:6543145-6543164 None:intergenic 55.0%
TAGACCTGCAGAAAGGGTGG+AGG - chr2.1:6543138-6543157 MS.gene45522:CDS 55.0%
TGACATTCAGCCAGTGACGG+TGG - chr2.1:6542388-6542407 MS.gene45522:CDS 55.0%
TGAGACGTACAAGCAGTGGC+AGG - chr2.1:6543606-6543625 MS.gene45522:CDS 55.0%
TGCCACTGCTTGTACGTCTC+AGG + chr2.1:6543607-6543626 None:intergenic 55.0%
!! CCTCTTGGTTTGGCAACTGC+TGG - chr2.1:6542878-6542897 MS.gene45522:CDS 55.0%
!! GCGCACCGTTTTTCCTGACT+CGG - chr2.1:6543425-6543444 MS.gene45522:CDS 55.0%
CGTACAAGCAGTGGCAGGTC+AGG - chr2.1:6543611-6543630 MS.gene45522:CDS 60.0%
GACCTCTGCGTGTCTTCACG+AGG + chr2.1:6543508-6543527 None:intergenic 60.0%
GGCCTGAGACGTACAAGCAG+TGG - chr2.1:6543602-6543621 MS.gene45522:CDS 60.0%
TGCCTCGTGAAGACACGCAG+AGG - chr2.1:6543503-6543522 MS.gene45522:CDS 60.0%
!! CGAAGCTTTGGAGGACCACC+AGG + chr2.1:6543085-6543104 None:intergenic 60.0%
TTCTGACGAGGCACGGCCAC+TGG + chr2.1:6543048-6543067 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 6541840 6543810 6541840 ID=MS.gene45522
chr2.1 mRNA 6541840 6543810 6541840 ID=MS.gene45522.t1;Parent=MS.gene45522
chr2.1 exon 6541840 6543810 6541840 ID=MS.gene45522.t1.exon1;Parent=MS.gene45522.t1
chr2.1 CDS 6541840 6543810 6541840 ID=cds.MS.gene45522.t1;Parent=MS.gene45522.t1
Gene Sequence

>MS.gene45522

ATGCTTTCTCCAAACACCTTTCTGCAAGATTTTAGTGTTCCAAACTCAGTAAACCAAGTAACAAATGGGTTTGAATTAGAAGATTCACCATCTTCACCTTCTTCTTCTGGCACAAGCTCAAATGGAGAATCCTTGGAACCGACAAGATATTCCAATAACATCCTAAGATACATAAGTGACATCCTCATGGATGAAGAAGATGACTTGGAGCAAAAACCCTGCATGTTGCAGGAATGTTTAAGACTTCAGGCTGCTGAAAAGTCCTTCTATGATGTTCTTGGCCATAACCATCCATCCCCAAATGGTAGCACTGACCCAGATGGAAATTTCGATCGTGCTGCTAGTTTTGAGAGAAATGGCAGCAGCTGCACCACTGATAACTCATATGAATCTAACTGGGTCAACCATGTTGTCGAATTCGACTCTTCTTCCATACAACTACAAACTCCTTTAATAGAAAAAAACTACTATGACTTGACAGAAGCTGATCCAGTTGTCATCGAGACACGAGGTGCTAACCATTGTCACAACGGAACTTGGAGTTGGAATGACATTCAGCCAGTGACGGTGGAAGAAGTTTCACCTTCAACAGTACCAGAAAAAAGAAGTCATAAGATGGATAATGATAATGATACTAGTAATGAACAAGAGGGAAGAGGTAGCAAGGTTTCAGCTATTCTCTCTGATGAATTAGAGCCACCAGAGATTTTAGATGAGGTACTGCTTTATCAGACAGGAAAGAGTCTCACTCAACACCAACAAACATCTTATAATTTTGATTCAGGAGGAAAGGCAAAGGAAACACATTCGCGTTTGAAGAAAGCATCTACTACGAACAATGTTGCGGCTGTTGATTTGTGGACCATGCTAAATCAATGTGCACAAGCTGTGGCGAGCTACGATCAAAGGAATACAGATGAGCTACTCAAACAGATTAGGCAACACTCTTCGCCTTTTGGCAATGGACTCCAACGTTTAGCTCATTACTTTGCTAATGGTCTTGAGATAAGATTGGCTGCTGAGACGCCATCATATCAGCCTCTTGGTTTGGCAACTGCTGGAGATATGTTGAAAGCTTACAAACTATTTGTTACTGCGTCTCCTTTGCAGAGAATGACAAATGCTTTGCTAACCAAAACGATTTTCAAAATAGTGAAGAACGAGAGCAGCGTTCATGTTATTGATTTTGGAATTTTCTATGGTTTCCAGTGGCCGTGCCTCGTCAGAAAACTTTCATTAAGACCTGGTGGTCCTCCAAAGCTTCGAATTACAGGAATTGAGCTTCCTCAGCCAGGATTTAGACCTGCAGAAAGGGTGGAGGAAACTGGACGGCGTCTAGCAAACTACTGCAAGAAGTTCAATGTACCATTTGAATACAACTTCATTGCACAGAAATGGGAAACCATCCGTCTCAAGGATCTCAAGATAGACAGTAATGAAATAACTCTTGTTAGCTGTTTGTACAGACTGAAGAACCTTCCTGATGAAACTGTGGCATTGAATTGTCCAAGAGAAGCTGTGTTGAAGCTGATCAGGAAGATTAATCCAAAAGTCTTCTTCCATGGAGTTGCCAATGGAAGTTATAGCGCACCGTTTTTCCTGACTCGGTTTAAGGAAGCACTCTACCACTTCTCATCGCTGTTTGATATGTTTGAGGTTAACGTGCCTCGTGAAGACACGCAGAGGTCGATGCTTGAGAGAGGGTTGTTTGGAAGGGATGCTATAAATGTTATAGCTTGTGAGGGAGCAGAAAGGGTTGAAAGGCCTGAGACGTACAAGCAGTGGCAGGTCAGGAACAAGAGAGCTGGGTTCAAGCAAATCAGGTTGGATTCTGATTTGGTGAATGAAACCAAAGCAATGGTGAAGAAGGAATACCATAAAGATTTTGTAGTCGATGTGGATGGTAAGTGGGTTTTAAAGGGATGGAAAGGACGCATTTTGTATGCTTTATCTGCTTGGGTTCCTGCTTAA
Protein sequence

>MS.gene45522.t1

MLSPNTFLQDFSVPNSVNQVTNGFELEDSPSSPSSSGTSSNGESLEPTRYSNNILRYISDILMDEEDDLEQKPCMLQECLRLQAAEKSFYDVLGHNHPSPNGSTDPDGNFDRAASFERNGSSCTTDNSYESNWVNHVVEFDSSSIQLQTPLIEKNYYDLTEADPVVIETRGANHCHNGTWSWNDIQPVTVEEVSPSTVPEKRSHKMDNDNDTSNEQEGRGSKVSAILSDELEPPEILDEVLLYQTGKSLTQHQQTSYNFDSGGKAKETHSRLKKASTTNNVAAVDLWTMLNQCAQAVASYDQRNTDELLKQIRQHSSPFGNGLQRLAHYFANGLEIRLAAETPSYQPLGLATAGDMLKAYKLFVTASPLQRMTNALLTKTIFKIVKNESSVHVIDFGIFYGFQWPCLVRKLSLRPGGPPKLRITGIELPQPGFRPAERVEETGRRLANYCKKFNVPFEYNFIAQKWETIRLKDLKIDSNEITLVSCLYRLKNLPDETVALNCPREAVLKLIRKINPKVFFHGVANGSYSAPFFLTRFKEALYHFSSLFDMFEVNVPREDTQRSMLERGLFGRDAINVIACEGAERVERPETYKQWQVRNKRAGFKQIRLDSDLVNETKAMVKKEYHKDFVVDVDGKWVLKGWKGRILYALSAWVPA