Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45751.t1 | XP_013445072.1 | 99.6 | 464 | 2 | 0 | 7 | 470 | 34 | 497 | 5.30E-274 | 953 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45751.t1 | Q6DKP5 | 37.0 | 330 | 198 | 7 | 141 | 463 | 158 | 484 | 3.6e-53 | 210.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45751.t1 | A0A072TQH3 | 99.6 | 464 | 2 | 0 | 7 | 470 | 34 | 497 | 3.8e-274 | 953.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059849 | MS.gene45751 | 0.807171 | 5.76E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene45751 | MS.gene51850 | PPI |
| MS.gene45751 | MS.gene022257 | PPI |
| MS.gene45751 | MS.gene074392 | PPI |
| MS.gene45751 | MS.gene27507 | PPI |
| MS.gene45751 | MS.gene70587 | PPI |
| MS.gene45751 | MS.gene87680 | PPI |
| MS.gene45751 | MS.gene92768 | PPI |
| MS.gene45751 | MS.gene25914 | PPI |
| MS.gene45751 | MS.gene036937 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45751.t1 | MTR_8g037340 | 99.571 | 466 | 2 | 0 | 5 | 470 | 32 | 497 | 0.0 | 970 |
| MS.gene45751.t1 | MTR_8g037340 | 93.548 | 496 | 2 | 1 | 5 | 470 | 32 | 527 | 0.0 | 954 |
| MS.gene45751.t1 | MTR_1g072140 | 28.182 | 330 | 205 | 13 | 139 | 462 | 12 | 315 | 1.58e-25 | 106 |
| MS.gene45751.t1 | MTR_4g119620 | 25.915 | 328 | 196 | 14 | 154 | 463 | 27 | 325 | 5.56e-19 | 88.2 |
| MS.gene45751.t1 | MTR_4g123597 | 21.557 | 334 | 227 | 8 | 154 | 459 | 106 | 432 | 3.90e-12 | 69.3 |
| MS.gene45751.t1 | MTR_2g079740 | 22.887 | 284 | 183 | 8 | 181 | 446 | 474 | 739 | 5.72e-11 | 65.5 |
| MS.gene45751.t1 | MTR_8g092890 | 21.849 | 238 | 167 | 6 | 181 | 417 | 276 | 495 | 5.92e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45751.t1 | AT2G16405 | 75.108 | 462 | 110 | 4 | 5 | 465 | 24 | 481 | 0.0 | 724 |
| MS.gene45751.t1 | AT3G49660 | 27.329 | 322 | 189 | 16 | 156 | 463 | 26 | 316 | 2.26e-21 | 95.1 |
| MS.gene45751.t1 | AT4G02730 | 25.208 | 361 | 229 | 15 | 110 | 462 | 5 | 332 | 2.55e-17 | 83.6 |
| MS.gene45751.t1 | AT5G53500 | 24.473 | 237 | 158 | 7 | 181 | 415 | 313 | 530 | 4.07e-12 | 68.9 |
| MS.gene45751.t1 | AT5G53500 | 24.473 | 237 | 158 | 7 | 181 | 415 | 313 | 530 | 4.07e-12 | 68.9 |
| MS.gene45751.t1 | AT5G54200 | 25.207 | 242 | 153 | 9 | 181 | 416 | 453 | 672 | 4.15e-12 | 68.9 |
Find 106 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCTTCTTTCTCTTGAAAA+AGG | 0.184205 | 8.3:-58962984 | MS.gene45751:CDS |
| GAGGTGGACAGCTGGAGTTC+TGG | 0.218453 | 8.3:-58967951 | MS.gene45751:CDS |
| AGTTCTGACCTACTTGCTTA+TGG | 0.246804 | 8.3:-58966621 | MS.gene45751:CDS |
| TGGATAAAACTGTGAGAGTA+TGG | 0.287100 | 8.3:-58965236 | MS.gene45751:CDS |
| ACATAAACAGGCCGAGGAAC+TGG | 0.321105 | 8.3:-58968715 | MS.gene45751:CDS |
| GTACATTGCTTCTGCTTCAT+TGG | 0.327705 | 8.3:-58965256 | MS.gene45751:CDS |
| AATTGCATGCGGGTGATATA+TGG | 0.338828 | 8.3:-58965198 | MS.gene45751:CDS |
| GTAGTTACGGTGATAAAGTT+TGG | 0.339409 | 8.3:-58966651 | MS.gene45751:intron |
| AAATTCAGCAACCTGTTTCC+TGG | 0.348373 | 8.3:+58968656 | None:intergenic |
| AGGTGGACAGCTGGAGTTCT+GGG | 0.349940 | 8.3:-58967950 | MS.gene45751:CDS |
| GGCATATTACAGTGTGGCTT+TGG | 0.361902 | 8.3:-58963084 | MS.gene45751:intron |
| AGAGTATGGGAGATAGAAAA+AGG | 0.367149 | 8.3:-58965222 | MS.gene45751:CDS |
| TCCAATCATAGAGCAAGTTT+TGG | 0.376757 | 8.3:-58966877 | MS.gene45751:CDS |
| TTTACGGCATAGTAATTGTT+TGG | 0.377298 | 8.3:-58962559 | MS.gene45751:CDS |
| TCGGCCTGTTTATGTAATTC+CGG | 0.380316 | 8.3:+58968723 | None:intergenic |
| CCTGGAGTACTTCGAAGACT+TGG | 0.393197 | 8.3:+58968674 | None:intergenic |
| GTTCTGACCTACTTGCTTAT+GGG | 0.394653 | 8.3:-58966620 | MS.gene45751:CDS |
| CAGTCACAACAGTGCAGTAT+AGG | 0.394700 | 8.3:-58963262 | MS.gene45751:CDS |
| CCGAGGAACTGGGAAAGTTT+AGG | 0.398546 | 8.3:-58968704 | MS.gene45751:CDS |
| TTGTTCACACATGTATGCTT+TGG | 0.408174 | 8.3:+58962733 | None:intergenic |
| AGTGTGGCTTTGGAGATAAA+AGG | 0.415343 | 8.3:-58963074 | MS.gene45751:CDS |
| CTGGAGTACTTCGAAGACTT+GGG | 0.427411 | 8.3:+58968675 | None:intergenic |
| GCCAAAACTTGCTCTATGAT+TGG | 0.428070 | 8.3:+58966876 | None:intergenic |
| GGGTCATCGGTTTCCTGTGA+TGG | 0.438655 | 8.3:-58962627 | MS.gene45751:CDS |
| TAGTTACGGTGATAAAGTTT+GGG | 0.440842 | 8.3:-58966650 | MS.gene45751:CDS |
| AGAAGACAGCAGAATAAGTC+TGG | 0.447786 | 8.3:+58969664 | None:intergenic |
| TCTGATCGTCCACGAATTCG+TGG | 0.450477 | 8.3:-58966832 | MS.gene45751:CDS |
| GACCAACGGGAGAGTGGACT+TGG | 0.450591 | 8.3:+58967975 | None:intergenic |
| TTCGAAGTACTCCAGGAAAC+AGG | 0.450607 | 8.3:-58968667 | MS.gene45751:intron |
| AAAATATGAGATGGCCAGTA+TGG | 0.452859 | 8.3:+58963469 | None:intergenic |
| ATGCATTCCTACGAAGGAAT+AGG | 0.453630 | 8.3:+58966798 | None:intergenic |
| CATACCGGAATTACATAAAC+AGG | 0.453670 | 8.3:-58968727 | MS.gene45751:CDS |
| TTCCTGTGATGGGGATTGCT+TGG | 0.458213 | 8.3:-58962616 | MS.gene45751:CDS |
| GGTCATCGGTTTCCTGTGAT+GGG | 0.461880 | 8.3:-58962626 | MS.gene45751:CDS |
| GTTGGTCTGAGGTGGACAGC+TGG | 0.471148 | 8.3:-58967959 | MS.gene45751:CDS |
| TCAATGGACCACGACCATAC+TGG | 0.471678 | 8.3:-58963483 | MS.gene45751:CDS |
| GCTGTCCACCTCAGACCAAC+GGG | 0.477029 | 8.3:+58967962 | None:intergenic |
| TCAGTCATCAAACAATTAAA+CGG | 0.482319 | 8.3:-58966552 | MS.gene45751:CDS |
| CCTAAACTTTCCCAGTTCCT+CGG | 0.484066 | 8.3:+58968704 | None:intergenic |
| GGAAAACTTACGTGGCATAC+CGG | 0.485124 | 8.3:-58968742 | MS.gene45751:CDS |
| TTCGACCAGTGCAAAGCCTC+TGG | 0.486329 | 8.3:-58966769 | MS.gene45751:intron |
| TTGTACTCACAGGGTGGAAA+CGG | 0.487559 | 8.3:+58965154 | None:intergenic |
| CGGGAGAGTGGACTTGGAGA+TGG | 0.503119 | 8.3:+58967981 | None:intergenic |
| CTATACTGCACTGTTGTGAC+TGG | 0.513927 | 8.3:+58963263 | None:intergenic |
| TTTAATTGTTTGATGACTGA+AGG | 0.515062 | 8.3:+58966555 | None:intergenic |
| CTTGCTTATGGGGCATCAGA+TGG | 0.516204 | 8.3:-58966609 | MS.gene45751:CDS |
| TAGATTTCCATCCTGAGTAC+AGG | 0.521762 | 8.3:+58963202 | None:intergenic |
| TGACGCCGAAGTTACCTCAA+TGG | 0.522944 | 8.3:-58963499 | MS.gene45751:CDS |
| GTGATGCGATGCAGGGTCAT+CGG | 0.522992 | 8.3:-58962640 | MS.gene45751:intron |
| TGAAGGATTGGAGATGCAAC+GGG | 0.524759 | 8.3:-58968763 | MS.gene45751:intron |
| GAGCCTGCCTATTCCTTCGT+AGG | 0.527204 | 8.3:-58966805 | MS.gene45751:CDS |
| ACTTCGATGACGATGTGAGC+GGG | 0.531444 | 8.3:+58963304 | None:intergenic |
| CATAAACAGGCCGAGGAACT+GGG | 0.531839 | 8.3:-58968714 | MS.gene45751:CDS |
| TTCTGACCTACTTGCTTATG+GGG | 0.543681 | 8.3:-58966619 | MS.gene45751:CDS |
| AGCTGGAATTCTCCGCCGTA+AGG | 0.553349 | 8.3:-58969569 | MS.gene45751:intron |
| TCCACTCTCCCGTTGGTCTG+AGG | 0.553452 | 8.3:-58967970 | MS.gene45751:CDS |
| CTCCAAGTCCACTCTCCCGT+TGG | 0.556019 | 8.3:-58967977 | MS.gene45751:CDS |
| ACTCTCCCGTTGGTCTGAGG+TGG | 0.557213 | 8.3:-58967967 | MS.gene45751:CDS |
| GGCTCAACACCACGAATTCG+TGG | 0.557690 | 8.3:+58966823 | None:intergenic |
| TGTGTTGTTGACCTGTACTC+AGG | 0.559101 | 8.3:-58963213 | MS.gene45751:CDS |
| CGACGAATGCCAGTGTACTC+AGG | 0.559149 | 8.3:+58969616 | None:intergenic |
| GAATAACAACTTCCTTTCTG+TGG | 0.561366 | 8.3:-58964262 | MS.gene45751:CDS |
| AGCTGTCCACCTCAGACCAA+CGG | 0.563191 | 8.3:+58967961 | None:intergenic |
| TGGATACTGAGTTTGCAACT+TGG | 0.564933 | 8.3:+58966896 | None:intergenic |
| AATAACAACTTCCTTTCTGT+GGG | 0.569279 | 8.3:-58964261 | MS.gene45751:CDS |
| CCAAGTCTTCGAAGTACTCC+AGG | 0.569413 | 8.3:-58968674 | MS.gene45751:CDS |
| TTCCAAGCAATCCCCATCAC+AGG | 0.577118 | 8.3:+58962614 | None:intergenic |
| TACATGTGTGAACAAGCTAC+AGG | 0.579845 | 8.3:-58962725 | MS.gene45751:intron |
| TACTACCAGAGGCTTTGCAC+TGG | 0.583440 | 8.3:+58966764 | None:intergenic |
| ATGGGGATTGCTTGGAACCA+TGG | 0.583684 | 8.3:-58962608 | MS.gene45751:CDS |
| TGTTTGCATTGCCCACAGAA+AGG | 0.583927 | 8.3:+58964250 | None:intergenic |
| TGAAACACTGCACACAGTCA+AGG | 0.586692 | 8.3:+58966584 | None:intergenic |
| GGATAAAACTGTGAGAGTAT+GGG | 0.586995 | 8.3:-58965235 | MS.gene45751:CDS |
| CTGATGCCCCATAAGCAAGT+AGG | 0.590315 | 8.3:+58966613 | None:intergenic |
| GGATCAGCGTCGGAGGTGAG+AGG | 0.592584 | 8.3:+58969637 | None:intergenic |
| TGAGATGGCCAGTATGGTCG+TGG | 0.593850 | 8.3:+58963475 | None:intergenic |
| TGGAGTACTTCGAAGACTTG+GGG | 0.596156 | 8.3:+58968676 | None:intergenic |
| CATCTCCACAAAATATGAGA+TGG | 0.597341 | 8.3:+58963460 | None:intergenic |
| ACCTCAGACCAACGGGAGAG+TGG | 0.599253 | 8.3:+58967969 | None:intergenic |
| GGACAGCTGGAGTTCTGGGA+GGG | 0.602771 | 8.3:-58967946 | MS.gene45751:intron |
| GCTCGCTCAAGTTAACTCCT+CGG | 0.603557 | 8.3:-58963034 | MS.gene45751:CDS |
| TCCTACACCATGGCGGAGCA+CGG | 0.604515 | 8.3:-58969772 | None:intergenic |
| GATGCAATGCATTCCTACGA+AGG | 0.607558 | 8.3:+58966792 | None:intergenic |
| GCGGGATAATATACCTGTGT+GGG | 0.610158 | 8.3:+58963322 | None:intergenic |
| TGAGTACAGGTCAACAACAC+AGG | 0.611720 | 8.3:+58963215 | None:intergenic |
| AGTATGGTCGTGGTCCATTG+AGG | 0.613871 | 8.3:+58963485 | None:intergenic |
| ATTCCTACGAAGGAATAGGC+AGG | 0.617187 | 8.3:+58966802 | None:intergenic |
| GTCATCGGTTTCCTGTGATG+GGG | 0.617591 | 8.3:-58962625 | MS.gene45751:CDS |
| TACTTCGATGACGATGTGAG+CGG | 0.618776 | 8.3:+58963303 | None:intergenic |
| GTGGTCCATTGAGGTAACTT+CGG | 0.625366 | 8.3:+58963494 | None:intergenic |
| TTGAAGGATTGGAGATGCAA+CGG | 0.629606 | 8.3:-58968764 | MS.gene45751:intron |
| AGTTTCTCTCTGCTAGCTCG+AGG | 0.630037 | 8.3:-58963239 | MS.gene45751:CDS |
| AGTGTACTCAGGATCAGCGT+CGG | 0.632113 | 8.3:+58969627 | None:intergenic |
| GTTGATGCATCAGTGGCCGA+TGG | 0.633147 | 8.3:-58968007 | MS.gene45751:intron |
| GGCCATTCAAAAGACATCAC+AGG | 0.639255 | 8.3:-58966531 | MS.gene45751:intron |
| GACGCTGATCCTGAGTACAC+TGG | 0.643851 | 8.3:-58969625 | MS.gene45751:CDS |
| TGGACAGCTGGAGTTCTGGG+AGG | 0.650084 | 8.3:-58967947 | MS.gene45751:CDS |
| GGAATTACATAAACAGGCCG+AGG | 0.650444 | 8.3:-58968721 | MS.gene45751:CDS |
| GTACTCAGGATCAGCGTCGG+AGG | 0.653901 | 8.3:+58969630 | None:intergenic |
| TTGTTGACCTGTACTCAGGA+TGG | 0.657122 | 8.3:-58963209 | MS.gene45751:CDS |
| GTAAATATGAACTCCCACAC+AGG | 0.663031 | 8.3:-58963335 | MS.gene45751:CDS |
| ATATGCAGTTGCTGGAAGTG+AGG | 0.669433 | 8.3:-58962788 | MS.gene45751:intron |
| GACTTGGAGATGGAAACCAT+CGG | 0.697706 | 8.3:+58967991 | None:intergenic |
| ATGCAACGGGAAAACTTACG+TGG | 0.702149 | 8.3:-58968750 | MS.gene45751:CDS |
| AGCGGGATAATATACCTGTG+TGG | 0.702369 | 8.3:+58963321 | None:intergenic |
| ATGCGGAGTATACCTTACGG+CGG | 0.798435 | 8.3:+58969557 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 58962523 | 58969785 | 58962523 | ID=MS.gene45751 |
| chr8.3 | mRNA | 58962523 | 58969785 | 58962523 | ID=MS.gene45751.t1;Parent=MS.gene45751 |
| chr8.3 | exon | 58969773 | 58969785 | 58969773 | ID=MS.gene45751.t1.exon1;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58969773 | 58969785 | 58969773 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58969570 | 58969688 | 58969570 | ID=MS.gene45751.t1.exon2;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58969570 | 58969688 | 58969570 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58968668 | 58968780 | 58968668 | ID=MS.gene45751.t1.exon3;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58968668 | 58968780 | 58968668 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58967947 | 58968016 | 58967947 | ID=MS.gene45751.t1.exon4;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58967947 | 58968016 | 58967947 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58966770 | 58966923 | 58966770 | ID=MS.gene45751.t1.exon5;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58966770 | 58966923 | 58966770 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58966532 | 58966669 | 58966532 | ID=MS.gene45751.t1.exon6;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58966532 | 58966669 | 58966532 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58965164 | 58965303 | 58965164 | ID=MS.gene45751.t1.exon7;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58965164 | 58965303 | 58965164 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58964239 | 58964286 | 58964239 | ID=MS.gene45751.t1.exon8;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58964239 | 58964286 | 58964239 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58963455 | 58963565 | 58963455 | ID=MS.gene45751.t1.exon9;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58963455 | 58963565 | 58963455 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58963191 | 58963372 | 58963191 | ID=MS.gene45751.t1.exon10;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58963191 | 58963372 | 58963191 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58962973 | 58963094 | 58962973 | ID=MS.gene45751.t1.exon11;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58962973 | 58963094 | 58962973 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58962726 | 58962802 | 58962726 | ID=MS.gene45751.t1.exon12;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58962726 | 58962802 | 58962726 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| chr8.3 | exon | 58962523 | 58962648 | 58962523 | ID=MS.gene45751.t1.exon13;Parent=MS.gene45751.t1 |
| chr8.3 | CDS | 58962523 | 58962648 | 58962523 | ID=cds.MS.gene45751.t1;Parent=MS.gene45751.t1 |
| Gene Sequence |
| Protein sequence |
