AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46209

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46209.t1 MTR_5g082830 98.243 683 12 0 1 683 1 683 0.0 1390
MS.gene46209.t1 MTR_5g071070 48.615 722 312 14 1 682 1 703 0.0 642
MS.gene46209.t1 MTR_5g071070 49.375 640 270 13 78 682 1 621 0.0 579
MS.gene46209.t1 MTR_1g075890 44.444 693 289 17 1 643 1 647 1.66e-175 516
MS.gene46209.t1 MTR_7g092070 43.034 725 302 21 30 683 47 731 1.00e-168 501
MS.gene46209.t1 MTR_3g070880 62.500 400 85 7 16 414 1 336 6.81e-166 486
MS.gene46209.t1 MTR_3g070880 57.143 203 62 8 474 674 336 515 5.47e-54 194
MS.gene46209.t1 MTR_3g029590 40.496 726 317 22 28 678 43 728 1.79e-146 444
MS.gene46209.t1 MTR_3g464260 46.712 441 158 10 33 463 28 401 7.13e-117 360
MS.gene46209.t1 MTR_3g464260 31.050 219 105 9 477 683 345 529 1.72e-13 73.9
MS.gene46209.t1 MTR_3g464280 38.924 632 236 19 1 624 1 490 2.45e-110 342
MS.gene46209.t1 MTR_1g050438 37.452 526 254 13 30 509 15 511 5.34e-104 329
MS.gene46209.t1 MTR_0728s0020 37.452 526 254 13 30 509 31 527 1.94e-103 328
MS.gene46209.t1 MTR_3g435590 43.902 410 195 7 1 385 1 400 8.04e-103 322
MS.gene46209.t1 MTR_3g435680 46.048 291 119 6 133 398 44 321 1.18e-74 246
MS.gene46209.t1 MTR_3g464290 36.008 511 209 17 150 640 39 451 6.35e-71 238
MS.gene46209.t1 MTR_3g464270 36.066 427 191 15 216 638 8 356 9.38e-59 202
MS.gene46209.t1 MTR_8g102100 48.826 213 100 4 213 420 77 285 1.98e-54 191
MS.gene46209.t1 MTR_6g090380 68.519 108 34 0 212 319 98 205 2.42e-51 182
MS.gene46209.t1 MTR_3g103960 52.795 161 61 3 207 366 43 189 6.79e-49 173
MS.gene46209.t1 MTR_2g090605 64.865 111 37 1 217 325 77 187 9.08e-48 174
MS.gene46209.t1 MTR_4g078660 60.748 107 42 0 213 319 56 162 7.01e-46 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46209.t1 AT2G40140 51.076 697 226 21 1 683 1 596 0.0 601
MS.gene46209.t1 AT2G40140 51.076 697 226 21 1 683 1 596 0.0 601
MS.gene46209.t1 AT5G58620 49.629 673 245 16 28 683 12 607 0.0 589
MS.gene46209.t1 AT3G55980 50.072 691 226 17 1 683 1 580 0.0 579
MS.gene46209.t1 AT3G55980 47.299 685 254 18 1 683 68 647 7.90e-179 524
MS.gene46209.t1 AT2G41900 40.393 713 319 19 30 683 48 713 3.52e-144 437
MS.gene46209.t1 AT5G12850 40.608 724 306 25 25 678 37 706 2.74e-143 435
MS.gene46209.t1 AT5G07500 62.698 126 47 0 194 319 29 154 3.92e-55 189
MS.gene46209.t1 AT4G29190 46.256 227 110 4 195 420 58 273 1.45e-54 191
MS.gene46209.t1 AT2G25900 68.224 107 34 0 213 319 86 192 4.03e-51 180
MS.gene46209.t1 AT2G25900 68.224 107 34 0 213 319 86 192 4.03e-51 180
MS.gene46209.t1 AT2G19810 63.559 118 43 0 202 319 63 180 1.10e-50 180
MS.gene46209.t1 AT1G03790 55.263 152 54 3 213 360 78 219 8.77e-49 176
MS.gene46209.t1 AT5G44260 62.963 108 39 1 213 319 56 163 3.70e-44 163

Find 204 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGAAGGTGGTTCATGTTT+TGG 0.196801 5.3:-66913701 None:intergenic
CTGAGGAAATCCGCTTCTTT+TGG 0.197101 5.3:+66914005 MS.gene46209:CDS
CTGCCACATGTTTCCACTCT+TGG 0.215837 5.3:-66913313 None:intergenic
ACTCGCAAGAATCCCCTTTC+TGG 0.236566 5.3:-66913005 None:intergenic
CTTATGATTGCTTCCTTGTT+TGG 0.250098 5.3:+66912421 MS.gene46209:CDS
GACATAAACAACGGTGTATA+TGG 0.256569 5.3:+66912811 MS.gene46209:CDS
CGATTTGGACAACGAATTAA+AGG 0.268967 5.3:-66912653 None:intergenic
ATCTGTTGTGTGGGTGTTGA+TGG 0.282762 5.3:-66913633 None:intergenic
TGTAGTTGAGATCGTAAATT+AGG 0.287165 5.3:-66913594 None:intergenic
GTCGTCCGGCCTTTCAGATT+GGG 0.298046 5.3:+66913929 MS.gene46209:CDS
TACACAGGGCGCAGCTCTTC+GGG 0.300339 5.3:-66913135 None:intergenic
TGTCGTCCGGCCTTTCAGAT+TGG 0.305721 5.3:+66913928 MS.gene46209:CDS
GCTCTTCATTGTGCTGTTGC+TGG 0.308167 5.3:+66912523 MS.gene46209:CDS
CAACACCTTCCTACGCGATT+TGG 0.321673 5.3:-66912668 None:intergenic
CGGAGTTTGTGGTTCCGATA+TGG 0.322011 5.3:+66912495 MS.gene46209:CDS
CACGACCCCTTGCGAAACTC+TGG 0.328143 5.3:-66912982 None:intergenic
TTGGGTTAATTCATTGGTTA+AGG 0.328824 5.3:+66914088 MS.gene46209:CDS
TGCCACATGTTTCCACTCTT+GGG 0.340074 5.3:-66913312 None:intergenic
AAGAATTGGATCGAAGAAAA+TGG 0.341157 5.3:+66912378 MS.gene46209:CDS
CAGCTGCAGTGATGAATTCT+AGG 0.343853 5.3:+66913805 MS.gene46209:CDS
TGTCTCTTGGGTTAATTCAT+TGG 0.351526 5.3:+66914082 MS.gene46209:CDS
TCTGAGAATTCCGGTGTATA+TGG 0.353483 5.3:+66914119 MS.gene46209:CDS
TTTGGCCCATGTTCATGTTT+TGG 0.358068 5.3:-66913683 None:intergenic
CGCCTTCCAACCCTTCCTGC+AGG 0.359580 5.3:+66913904 MS.gene46209:CDS
ATACACAGGGCGCAGCTCTT+CGG 0.370872 5.3:-66913136 None:intergenic
TATATGGATCAGATGAGTTT+AGG 0.370932 5.3:+66912827 MS.gene46209:CDS
AGAAGTAGATGAGAAGGGTT+TGG 0.379368 5.3:+66912324 MS.gene46209:CDS
GACATCAGGCTCAGCATGTT+GGG 0.385432 5.3:-66914063 None:intergenic
CTCCTCCTTGCATTCTCACC+TGG 0.394967 5.3:-66912922 None:intergenic
TAGGATCAGCTGATGCTAAT+AGG 0.402231 5.3:-66913575 None:intergenic
ATGCTGAGCCTGATGTCTCT+TGG 0.404935 5.3:+66914069 MS.gene46209:CDS
AATAGGTCATCAAGGTTAGT+TGG 0.407177 5.3:-66913558 None:intergenic
CCCCACTGGGAAACTCGATT+GGG 0.408358 5.3:+66913956 MS.gene46209:CDS
TTCTGTTGAATTCGCTGTTA+CGG 0.410177 5.3:-66913521 None:intergenic
GTCAATTCCACTAGCTGAAT+AGG 0.414997 5.3:-66913193 None:intergenic
GAGGAAGGCATATTGTTTGA+TGG 0.415999 5.3:-66913732 None:intergenic
TGGGATGCAGTTGGTGCTGC+TGG 0.418675 5.3:-66914044 None:intergenic
GTCAAGCTCTTGCTTGATGC+CGG 0.418825 5.3:+66912571 MS.gene46209:CDS
TATGGTGAGGGCTTCCTCAC+TGG 0.419808 5.3:-66913756 None:intergenic
CGCAAGGGGTCGTGCCAGAA+AGG 0.421946 5.3:+66912991 MS.gene46209:CDS
CGTCCTTGCAAAGCCTTGTT+CGG 0.424614 5.3:-66913071 None:intergenic
CAGCTGTAAGGGTATTTCCT+TGG 0.426047 5.3:-66912949 None:intergenic
ATTGATGTCAACGGAGTTTG+TGG 0.428144 5.3:+66912484 MS.gene46209:CDS
TCGGGCAACGATATATCAAT+AGG 0.428410 5.3:-66912790 None:intergenic
GTCGAGTATATTCTTTCGAC+AGG 0.429694 5.3:+66912457 MS.gene46209:CDS
CTGCAGCTGCCGCATATTCT+CGG 0.430426 5.3:-66914144 None:intergenic
GTGAGGAAGCCCTCACCATA+TGG 0.432783 5.3:+66913759 MS.gene46209:CDS
GCACTCTTCAAACGGCTACC+TGG 0.432870 5.3:-66913372 None:intergenic
CGCCCTGTGTATGCCTCCAC+TGG 0.433195 5.3:+66913147 MS.gene46209:CDS
AGCTGTAAGGGTATTTCCTT+GGG 0.442755 5.3:-66912948 None:intergenic
CCACTAGCTGAATAGGACTT+TGG 0.443786 5.3:-66913186 None:intergenic
CCCCTTGCGAAACTCTGGGC+AGG 0.444998 5.3:-66912977 None:intergenic
GATGTTCCATCTGAGAATTC+CGG 0.446112 5.3:+66914110 MS.gene46209:CDS
AATTCGCTGTTACGGAAAGA+TGG 0.453909 5.3:-66913513 None:intergenic
GGGCAGGGAACACAGCTGTA+AGG 0.457289 5.3:-66912961 None:intergenic
GTTTAGGATGTACAGTTTCA+AGG 0.458638 5.3:+66912843 MS.gene46209:CDS
ATGTGAATGAAGAAGGCTTT+TGG 0.459210 5.3:+66912347 MS.gene46209:CDS
TGCTGAGCCTGATGTCTCTT+GGG 0.461989 5.3:+66914070 MS.gene46209:CDS
TATACACCGTTGTTTATGTC+GGG 0.462321 5.3:-66912808 None:intergenic
CACTCAAAGCACTCTTCAAA+CGG 0.468740 5.3:-66913380 None:intergenic
TGAGATGGAATTGCTCGGTC+TGG 0.469920 5.3:+66913419 MS.gene46209:CDS
GGCTTCCTCACTGGAGAGGA+AGG 0.472160 5.3:-66913747 None:intergenic
CTACCTGGGAGCTGCAACGA+TGG 0.472506 5.3:-66913357 None:intergenic
CAAGAGAGAAGTAGATGAGA+AGG 0.474046 5.3:+66912318 MS.gene46209:CDS
TGGCGCATCTGCATTGCACT+TGG 0.475619 5.3:-66913663 None:intergenic
TTCTCACCTGGATGAACAAA+GGG 0.476487 5.3:-66912910 None:intergenic
AAGAGAGAAGTAGATGAGAA+GGG 0.478152 5.3:+66912319 MS.gene46209:CDS
AACAAGGAAGCAATCATAAG+AGG 0.482396 5.3:-66912418 None:intergenic
CATGGAGGATAAGCTTCAAA+AGG 0.484482 5.3:+66912237 MS.gene46209:CDS
GCTGGCGCAATCAGATCAAC+CGG 0.486158 5.3:-66912631 None:intergenic
CACTCTTCAAACGGCTACCT+GGG 0.486258 5.3:-66913371 None:intergenic
AGGCTTTGCAAGGACGAGAC+TGG 0.489002 5.3:+66913078 MS.gene46209:CDS
CACCACGGTCAATGAAACTT+TGG 0.491260 5.3:-66913854 None:intergenic
GCAAGGGGTCGTGCCAGAAA+GGG 0.492458 5.3:+66912992 MS.gene46209:CDS
GGTGCTGCAACTCACCATCT+TGG 0.497686 5.3:+66913873 MS.gene46209:CDS
GGTTTGGATGTGAATGAAGA+AGG 0.500120 5.3:+66912340 MS.gene46209:CDS
CCTTCCTGCAGGGTGTCGTC+CGG 0.501860 5.3:+66913915 MS.gene46209:CDS
AACACCACCCATGTCTCCCT+TGG 0.506352 5.3:+66913275 MS.gene46209:CDS
AAACTCGATTGGGGTGTAAA+CGG 0.506355 5.3:+66913966 MS.gene46209:CDS
ATGAAGAGCAGTGGCCATAT+CGG 0.506470 5.3:-66912509 None:intergenic
CACTCTAGTGCTTCCAAACA+AGG 0.508719 5.3:-66912434 None:intergenic
TGAAGAGTGCTTTGAGTGCA+AGG 0.510749 5.3:+66913385 MS.gene46209:CDS
TTGGCTGCGCTGCTTTGCAA+AGG 0.511458 5.3:-66913835 None:intergenic
CTTCATTGTGCTGTTGCTGG+TGG 0.512690 5.3:+66912526 MS.gene46209:CDS
TCGTCCGGCCTTTCAGATTG+GGG 0.513214 5.3:+66913930 MS.gene46209:CDS
AGCCTTGTTCGGTATTGTGC+GGG 0.515045 5.3:-66913060 None:intergenic
GCCTTCCAACCCTTCCTGCA+GGG 0.517193 5.3:+66913905 MS.gene46209:CDS
CCACCCATGTCTCCCTTGGC+AGG 0.517296 5.3:+66913279 MS.gene46209:CDS
AGACATCAGGCTCAGCATGT+TGG 0.517932 5.3:-66914064 None:intergenic
TTGTACATCTGCTCCACCCA+TGG 0.518688 5.3:-66914197 None:intergenic
TGATGCTAGTGGAAACAAAC+CGG 0.520501 5.3:+66912612 MS.gene46209:CDS
CCCTGCCCAGAGTTTCGCAA+GGG 0.522429 5.3:+66912976 MS.gene46209:CDS
TCGAGACGTGCTTCCACCAT+GGG 0.522786 5.3:+66914181 MS.gene46209:CDS
TTCATCCAGGTGAGAATGCA+AGG 0.523324 5.3:+66912917 MS.gene46209:CDS
ATTCGCTGTTACGGAAAGAT+GGG 0.523731 5.3:-66913512 None:intergenic
TCCCTGCCCAGAGTTTCGCA+AGG 0.526817 5.3:+66912975 MS.gene46209:CDS
GGAACCCCAATCTGAAAGGC+CGG 0.526956 5.3:-66913934 None:intergenic
CGACACCCTGCAGGAAGGGT+TGG 0.530365 5.3:-66913910 None:intergenic
TGTGGGTGTTGATGGTTGCA+TGG 0.532531 5.3:-66913625 None:intergenic
GTACAAAGAGCAGGAGCAGA+TGG 0.534247 5.3:+66914214 MS.gene46209:CDS
CCACCATCGTTGCAGCTCCC+AGG 0.534409 5.3:+66913354 MS.gene46209:CDS
GAATTAACCCAAGAGACATC+AGG 0.537150 5.3:-66914077 None:intergenic
GGAGCAGATGTACAAAGAGC+AGG 0.537466 5.3:+66914205 MS.gene46209:CDS
ACGACCCCTTGCGAAACTCT+GGG 0.538237 5.3:-66912981 None:intergenic
CGGACGACACCCTGCAGGAA+GGG 0.538863 5.3:-66913914 None:intergenic
CCAAAGTCCTATTCAGCTAG+TGG 0.541639 5.3:+66913186 MS.gene46209:CDS
TCAAGGTGAAGCCATGCTCA+AGG 0.542484 5.3:+66912860 MS.gene46209:CDS
GAGTGTCCCTTTGTTCATCC+AGG 0.543113 5.3:+66912904 MS.gene46209:CDS
AGAAAATGGAATCCGAAAAG+AGG 0.548081 5.3:+66912392 MS.gene46209:CDS
ATTTCTGAACCCAAAAGAAG+CGG 0.548536 5.3:-66914015 None:intergenic
GTTGTCCAAATCGCGTAGGA+AGG 0.549554 5.3:+66912663 MS.gene46209:CDS
GGGAGATGATGAACCTGCCA+AGG 0.553827 5.3:-66913292 None:intergenic
ACCCTGCAGGAAGGGTTGGA+AGG 0.554901 5.3:-66913906 None:intergenic
TTGGACAACGAATTAAAGGC+TGG 0.557548 5.3:-66912649 None:intergenic
TGGAGAGTGCATCTGTTGTG+TGG 0.558672 5.3:-66913643 None:intergenic
GGCAGGGAACACAGCTGTAA+GGG 0.559340 5.3:-66912960 None:intergenic
ATATACACCGTTGTTTATGT+CGG 0.561652 5.3:-66912809 None:intergenic
GCTGATGAATCAAATCCATA+TGG 0.562833 5.3:-66913774 None:intergenic
AAGCCTTGTTCGGTATTGTG+CGG 0.563056 5.3:-66913061 None:intergenic
TTCTTCATCAGACTGTGCCA+TGG 0.563892 5.3:+66912219 MS.gene46209:CDS
GAACCAGTGGAGGCATACAC+AGG 0.565086 5.3:-66913150 None:intergenic
CATCACTGCAGCTGCTACTG+CGG 0.565349 5.3:-66913796 None:intergenic
AAGGCCGGACGACACCCTGC+AGG 0.568796 5.3:-66913919 None:intergenic
GATCTTGAGATGGAATTGCT+CGG 0.572830 5.3:+66913414 MS.gene46209:CDS
CCTGGGAGCTGCAACGATGG+TGG 0.572925 5.3:-66913354 None:intergenic
GATGCGCCAAAACATGAACA+TGG 0.573046 5.3:+66913677 MS.gene46209:CDS
AGACGTGCTTCCACCATGGG+TGG 0.573955 5.3:+66914184 MS.gene46209:CDS
AATTTACGATCTCAACTACA+TGG 0.575156 5.3:+66913597 MS.gene46209:CDS
CTTGTTTGGAAGCACTAGAG+TGG 0.576155 5.3:+66912435 MS.gene46209:CDS
CCCTTGCGAAACTCTGGGCA+GGG 0.576535 5.3:-66912976 None:intergenic
TGAACCTGCCAAGGGAGACA+TGG 0.576779 5.3:-66913283 None:intergenic
AGACAATTCAAGCAAGATTG+AGG 0.577524 5.3:-66912272 None:intergenic
ATGGAGGATAAGCTTCAAAA+GGG 0.579380 5.3:+66912238 MS.gene46209:CDS
ATCCCGCACAATACCGAACA+AGG 0.579739 5.3:+66913058 MS.gene46209:CDS
AAGACAATCAGCATCTGCAC+CGG 0.580324 5.3:-66912590 None:intergenic
CAAGGGCTTACTCTCACGAT+TGG 0.580426 5.3:+66912878 MS.gene46209:CDS
GTCGAGACGTGCTTCCACCA+TGG 0.580527 5.3:+66914180 MS.gene46209:CDS
GGAGAGTGCATCTGTTGTGT+GGG 0.581542 5.3:-66913642 None:intergenic
ATGGATAACTTGCGCGAAGG+TGG 0.584205 5.3:-66913713 None:intergenic
AAGGAGCGTCCATCTCCCCT+TGG 0.584326 5.3:-66912729 None:intergenic
TGAACTCATCCAAGGGGAGA+TGG 0.584869 5.3:+66912720 MS.gene46209:CDS
CAAATCGCGTAGGAAGGTGT+TGG 0.585250 5.3:+66912669 MS.gene46209:CDS
GCTGATTGTCTTGATGCTAG+TGG 0.585414 5.3:+66912601 MS.gene46209:CDS
CTGATGCTAATAGGTCATCA+AGG 0.586548 5.3:-66913566 None:intergenic
CCGGACGACACCCTGCAGGA+AGG 0.586791 5.3:-66913915 None:intergenic
GAAGAGTGCTTTGAGTGCAA+GGG 0.591839 5.3:+66913386 MS.gene46209:CDS
CAAGGTGAAGCCATGCTCAA+GGG 0.594043 5.3:+66912861 MS.gene46209:CDS
CAAGGGGTCGTGCCAGAAAG+GGG 0.599774 5.3:+66912993 MS.gene46209:CDS
ATACCGAACAAGGCTTTGCA+AGG 0.600596 5.3:+66913068 MS.gene46209:CDS
TGCTGCTGCTGTTGACGACG+AGG 0.602651 5.3:-66913447 None:intergenic
GAATCAAATCCATATGGTGA+GGG 0.607337 5.3:-66913768 None:intergenic
AGGATTTGGATTATGTGCAG+TGG 0.608930 5.3:+66912181 None:intergenic
GTAAGTGCTGAACTCATCCA+AGG 0.609847 5.3:+66912712 MS.gene46209:CDS
TGAGAGTAAGCCCTTGAGCA+TGG 0.609898 5.3:-66912871 None:intergenic
CCTGCCCAGAGTTTCGCAAG+GGG 0.612209 5.3:+66912977 MS.gene46209:CDS
ATTCTCACCTGGATGAACAA+AGG 0.616516 5.3:-66912911 None:intergenic
TGGATGAACAAAGGGACACT+CGG 0.617153 5.3:-66912902 None:intergenic
GGTTCATCATCTCCCAAGAG+TGG 0.617657 5.3:+66913300 MS.gene46209:CDS
CTGCAGGAAGGGTTGGAAGG+CGG 0.621056 5.3:-66913903 None:intergenic
GGTGTTGAAACAGTTGACAT+AGG 0.621294 5.3:-66913258 None:intergenic
ATTCGTTGTCCAAATCGCGT+AGG 0.621353 5.3:+66912659 MS.gene46209:CDS
TCAGCATGTTGGGATGCAGT+TGG 0.624317 5.3:-66914053 None:intergenic
TGAATCAAATCCATATGGTG+AGG 0.627563 5.3:-66913769 None:intergenic
GCTGAATAGGACTTTGGTGA+AGG 0.628923 5.3:-66913180 None:intergenic
TATACACCGGAATTCTCAGA+TGG 0.629638 5.3:-66914116 None:intergenic
AGGCATAGCTGAACCAGTGG+AGG 0.631941 5.3:-66913160 None:intergenic
CCCACTGGGAAACTCGATTG+GGG 0.632471 5.3:+66913957 MS.gene46209:CDS
TACATCTGCTCCACCCATGG+TGG 0.632978 5.3:-66914194 None:intergenic
ATGCAAGGAGGAGAGACCCA+AGG 0.635278 5.3:+66912932 MS.gene46209:CDS
GGAGATGATGAACCTGCCAA+GGG 0.637245 5.3:-66913291 None:intergenic
GAACCTGCCAAGGGAGACAT+GGG 0.638207 5.3:-66913282 None:intergenic
AGCCAAAGTTTCATTGACCG+TGG 0.638346 5.3:+66913852 MS.gene46209:CDS
TATATGGTGCCGAGAATATG+CGG 0.638645 5.3:+66914135 MS.gene46209:CDS
TGAGGGCTTCCTCACTGGAG+AGG 0.638650 5.3:-66913751 None:intergenic
TTCATCAGACTGTGCCATGG+AGG 0.642039 5.3:+66912222 MS.gene46209:CDS
AACAGCACAATGAAGAGCAG+TGG 0.643629 5.3:-66912518 None:intergenic
TAAGTGCTGAACTCATCCAA+GGG 0.645605 5.3:+66912713 MS.gene46209:CDS
AACCAGTGGAGGCATACACA+GGG 0.645864 5.3:-66913149 None:intergenic
CCCCAATCGAGTTTCCCAGT+GGG 0.652420 5.3:-66913957 None:intergenic
GCTGCAACGATGGTGGAGTG+AGG 0.652628 5.3:-66913347 None:intergenic
ACAGGGAAGATTGATGTCAA+CGG 0.653698 5.3:+66912475 MS.gene46209:CDS
CCCAATCGAGTTTCCCAGTG+GGG 0.653880 5.3:-66913956 None:intergenic
ATGCGCCAAAACATGAACAT+GGG 0.661793 5.3:+66913678 MS.gene46209:CDS
CCAATCGAGTTTCCCAGTGG+GGG 0.662085 5.3:-66913955 None:intergenic
CTCCCAAGAGTGGAAACATG+TGG 0.663242 5.3:+66913310 MS.gene46209:CDS
AAGGGATCTCGATCTTGAGA+TGG 0.663328 5.3:+66913404 MS.gene46209:CDS
TGAAGGCATAGCTGAACCAG+TGG 0.664738 5.3:-66913163 None:intergenic
GCTGTTACGGAAAGATGGGG+AGG 0.665128 5.3:-66913508 None:intergenic
ACCCCAATCGAGTTTCCCAG+TGG 0.665168 5.3:-66913958 None:intergenic
AAGGCGGAGAAAGTCCAAGA+TGG 0.665195 5.3:-66913887 None:intergenic
GATTCTTGCGAGTATGCACA+TGG 0.665419 5.3:+66913015 MS.gene46209:CDS
TTCGCTGTTACGGAAAGATG+GGG 0.672071 5.3:-66913511 None:intergenic
GTGACGAGCTGAACAAGCTG+AGG 0.679618 5.3:+66913988 MS.gene46209:CDS
GATGAGGAGCTAAGAGACAA+CGG 0.680167 5.3:-66913231 None:intergenic
GATGGTGAGTTGCAGCACCA+CGG 0.682458 5.3:-66913869 None:intergenic
AGTTGACATAGGCAAAGATG+AGG 0.688585 5.3:-66913247 None:intergenic
ATTCTCGGCACCATATACAC+CGG 0.692075 5.3:-66914129 None:intergenic
TCGAGTATATTCTTTCGACA+GGG 0.693087 5.3:+66912458 MS.gene46209:CDS
TTGATGGATAACTTGCGCGA+AGG 0.700054 5.3:-66913716 None:intergenic
AAGTGCTGAACTCATCCAAG+GGG 0.716976 5.3:+66912714 MS.gene46209:CDS
ATATGCCTTCCTCTCCAGTG+AGG 0.717918 5.3:+66913742 MS.gene46209:CDS
TCGTTGCCCGACATAAACAA+CGG 0.735153 5.3:+66912802 MS.gene46209:CDS
CCTGCCAAGGGAGACATGGG+TGG 0.744203 5.3:-66913279 None:intergenic
ATCCAGGTGAGAATGCAAGG+AGG 0.764804 5.3:+66912920 MS.gene46209:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAATTGGATCGAAGAAAA+TGG + chr5.3:66912378-66912397 MS.gene46209:CDS 30.0%
AATTTACGATCTCAACTACA+TGG + chr5.3:66913597-66913616 MS.gene46209:CDS 30.0%
ATATACACCGTTGTTTATGT+CGG - chr5.3:66912812-66912831 None:intergenic 30.0%
CCTTTCTCATTGAAAAAAGA+AGG + chr5.3:66912748-66912767 MS.gene46209:CDS 30.0%
TATATGGATCAGATGAGTTT+AGG + chr5.3:66912827-66912846 MS.gene46209:CDS 30.0%
TGTAGTTGAGATCGTAAATT+AGG - chr5.3:66913597-66913616 None:intergenic 30.0%
TTCTCATTGAAAAAAGAAGG+TGG + chr5.3:66912751-66912770 MS.gene46209:CDS 30.0%
! TTGGGTTAATTCATTGGTTA+AGG + chr5.3:66914088-66914107 MS.gene46209:CDS 30.0%
! TTTTGGTATGGTAGAAGAAT+TGG + chr5.3:66912364-66912383 MS.gene46209:CDS 30.0%
!!! CCTTCTTTTTTCAATGAGAA+AGG - chr5.3:66912751-66912770 None:intergenic 30.0%
!!! TCTTTTGGGTTCAGAAATAA+TGG + chr5.3:66914020-66914039 MS.gene46209:CDS 30.0%
AACAAGGAAGCAATCATAAG+AGG - chr5.3:66912421-66912440 None:intergenic 35.0%
AAGAGAGAAGTAGATGAGAA+GGG + chr5.3:66912319-66912338 MS.gene46209:CDS 35.0%
AATAGGTCATCAAGGTTAGT+TGG - chr5.3:66913561-66913580 None:intergenic 35.0%
AGAAAATGGAATCCGAAAAG+AGG + chr5.3:66912392-66912411 MS.gene46209:CDS 35.0%
AGACAATTCAAGCAAGATTG+AGG - chr5.3:66912275-66912294 None:intergenic 35.0%
ATGGAGGATAAGCTTCAAAA+GGG + chr5.3:66912238-66912257 MS.gene46209:CDS 35.0%
ATTTCTGAACCCAAAAGAAG+CGG - chr5.3:66914018-66914037 None:intergenic 35.0%
CTTATGATTGCTTCCTTGTT+TGG + chr5.3:66912421-66912440 MS.gene46209:CDS 35.0%
GAATCAAATCCATATGGTGA+GGG - chr5.3:66913771-66913790 None:intergenic 35.0%
GACATAAACAACGGTGTATA+TGG + chr5.3:66912811-66912830 MS.gene46209:CDS 35.0%
GCTGATGAATCAAATCCATA+TGG - chr5.3:66913777-66913796 None:intergenic 35.0%
TATACACCGTTGTTTATGTC+GGG - chr5.3:66912811-66912830 None:intergenic 35.0%
TCGAGTATATTCTTTCGACA+GGG + chr5.3:66912458-66912477 MS.gene46209:CDS 35.0%
TGAATCAAATCCATATGGTG+AGG - chr5.3:66913772-66913791 None:intergenic 35.0%
TGTCTCTTGGGTTAATTCAT+TGG + chr5.3:66914082-66914101 MS.gene46209:CDS 35.0%
TTCTGTTGAATTCGCTGTTA+CGG - chr5.3:66913524-66913543 None:intergenic 35.0%
! AATGAAGAAGGCTTTTGGTA+TGG + chr5.3:66912352-66912371 MS.gene46209:CDS 35.0%
! ATGTGAATGAAGAAGGCTTT+TGG + chr5.3:66912347-66912366 MS.gene46209:CDS 35.0%
! CGATTTGGACAACGAATTAA+AGG - chr5.3:66912656-66912675 None:intergenic 35.0%
! GTTTAGGATGTACAGTTTCA+AGG + chr5.3:66912843-66912862 MS.gene46209:CDS 35.0%
AAACTCGATTGGGGTGTAAA+CGG + chr5.3:66913966-66913985 MS.gene46209:CDS 40.0%
AATTCGCTGTTACGGAAAGA+TGG - chr5.3:66913516-66913535 None:intergenic 40.0%
ACAGGGAAGATTGATGTCAA+CGG + chr5.3:66912475-66912494 MS.gene46209:CDS 40.0%
AGAAGTAGATGAGAAGGGTT+TGG + chr5.3:66912324-66912343 MS.gene46209:CDS 40.0%
AGTTGACATAGGCAAAGATG+AGG - chr5.3:66913250-66913269 None:intergenic 40.0%
ATGCGCCAAAACATGAACAT+GGG + chr5.3:66913678-66913697 MS.gene46209:CDS 40.0%
ATTCGCTGTTACGGAAAGAT+GGG - chr5.3:66913515-66913534 None:intergenic 40.0%
ATTCTCACCTGGATGAACAA+AGG - chr5.3:66912914-66912933 None:intergenic 40.0%
ATTGATGTCAACGGAGTTTG+TGG + chr5.3:66912484-66912503 MS.gene46209:CDS 40.0%
CAAGAGAGAAGTAGATGAGA+AGG + chr5.3:66912318-66912337 MS.gene46209:CDS 40.0%
CATGGAGGATAAGCTTCAAA+AGG + chr5.3:66912237-66912256 MS.gene46209:CDS 40.0%
CTGATGCTAATAGGTCATCA+AGG - chr5.3:66913569-66913588 None:intergenic 40.0%
GAATTAACCCAAGAGACATC+AGG - chr5.3:66914080-66914099 None:intergenic 40.0%
GATCTTGAGATGGAATTGCT+CGG + chr5.3:66913414-66913433 MS.gene46209:CDS 40.0%
GATGTTCCATCTGAGAATTC+CGG + chr5.3:66914110-66914129 MS.gene46209:CDS 40.0%
GGTTTGGATGTGAATGAAGA+AGG + chr5.3:66912340-66912359 MS.gene46209:CDS 40.0%
GTCAATTCCACTAGCTGAAT+AGG - chr5.3:66913196-66913215 None:intergenic 40.0%
GTCGAGTATATTCTTTCGAC+AGG + chr5.3:66912457-66912476 MS.gene46209:CDS 40.0%
TAGGATCAGCTGATGCTAAT+AGG - chr5.3:66913578-66913597 None:intergenic 40.0%
TATACACCGGAATTCTCAGA+TGG - chr5.3:66914119-66914138 None:intergenic 40.0%
TCGGGCAACGATATATCAAT+AGG - chr5.3:66912793-66912812 None:intergenic 40.0%
TCTGAGAATTCCGGTGTATA+TGG + chr5.3:66914119-66914138 MS.gene46209:CDS 40.0%
TGATGCTAGTGGAAACAAAC+CGG + chr5.3:66912612-66912631 MS.gene46209:CDS 40.0%
TTCTCACCTGGATGAACAAA+GGG - chr5.3:66912913-66912932 None:intergenic 40.0%
TTGGACAACGAATTAAAGGC+TGG - chr5.3:66912652-66912671 None:intergenic 40.0%
! CACTCAAAGCACTCTTCAAA+CGG - chr5.3:66913383-66913402 None:intergenic 40.0%
! CATAAGAGGAGTCCTCTTTT+CGG - chr5.3:66912407-66912426 None:intergenic 40.0%
! GAGGAAGGCATATTGTTTGA+TGG - chr5.3:66913735-66913754 None:intergenic 40.0%
! GGTGTTGAAACAGTTGACAT+AGG - chr5.3:66913261-66913280 None:intergenic 40.0%
! TATATGGTGCCGAGAATATG+CGG + chr5.3:66914135-66914154 MS.gene46209:CDS 40.0%
! TGAGGAAATCCGCTTCTTTT+GGG + chr5.3:66914006-66914025 MS.gene46209:CDS 40.0%
! TTTGGCCCATGTTCATGTTT+TGG - chr5.3:66913686-66913705 None:intergenic 40.0%
!! AGCTGTAAGGGTATTTCCTT+GGG - chr5.3:66912951-66912970 None:intergenic 40.0%
!! GTGTTGGAGATGTTTTTGAG+AGG + chr5.3:66912685-66912704 MS.gene46209:CDS 40.0%
!! TAAGTGCTGAACTCATCCAA+GGG + chr5.3:66912713-66912732 MS.gene46209:CDS 40.0%
!!! CTTTTGAAGCTTATCCTCCA+TGG - chr5.3:66912239-66912258 None:intergenic 40.0%
AACAGCACAATGAAGAGCAG+TGG - chr5.3:66912521-66912540 None:intergenic 45.0%
AAGACAATCAGCATCTGCAC+CGG - chr5.3:66912593-66912612 None:intergenic 45.0%
AAGGGATCTCGATCTTGAGA+TGG + chr5.3:66913404-66913423 MS.gene46209:CDS 45.0%
AGCCAAAGTTTCATTGACCG+TGG + chr5.3:66913852-66913871 MS.gene46209:CDS 45.0%
ATACCGAACAAGGCTTTGCA+AGG + chr5.3:66913068-66913087 MS.gene46209:CDS 45.0%
ATGAAGAGCAGTGGCCATAT+CGG - chr5.3:66912512-66912531 None:intergenic 45.0%
ATTCGTTGTCCAAATCGCGT+AGG + chr5.3:66912659-66912678 MS.gene46209:CDS 45.0%
ATTCTCGGCACCATATACAC+CGG - chr5.3:66914132-66914151 None:intergenic 45.0%
CACCACGGTCAATGAAACTT+TGG - chr5.3:66913857-66913876 None:intergenic 45.0%
CACTCTAGTGCTTCCAAACA+AGG - chr5.3:66912437-66912456 None:intergenic 45.0%
CAGCTGCAGTGATGAATTCT+AGG + chr5.3:66913805-66913824 MS.gene46209:CDS 45.0%
CCAAAGTCCTATTCAGCTAG+TGG + chr5.3:66913186-66913205 MS.gene46209:CDS 45.0%
CCACTAGCTGAATAGGACTT+TGG - chr5.3:66913189-66913208 None:intergenic 45.0%
GATGAGGAGCTAAGAGACAA+CGG - chr5.3:66913234-66913253 None:intergenic 45.0%
GATGCGCCAAAACATGAACA+TGG + chr5.3:66913677-66913696 MS.gene46209:CDS 45.0%
GATTCTTGCGAGTATGCACA+TGG + chr5.3:66913015-66913034 MS.gene46209:CDS 45.0%
GCTGAATAGGACTTTGGTGA+AGG - chr5.3:66913183-66913202 None:intergenic 45.0%
GCTGATTGTCTTGATGCTAG+TGG + chr5.3:66912601-66912620 MS.gene46209:CDS 45.0%
TCGTTGCCCGACATAAACAA+CGG + chr5.3:66912802-66912821 MS.gene46209:CDS 45.0%
TGCCACATGTTTCCACTCTT+GGG - chr5.3:66913315-66913334 None:intergenic 45.0%
TGGATGAACAAAGGGACACT+CGG - chr5.3:66912905-66912924 None:intergenic 45.0%
TTCATCCAGGTGAGAATGCA+AGG + chr5.3:66912917-66912936 MS.gene46209:CDS 45.0%
TTCGCTGTTACGGAAAGATG+GGG - chr5.3:66913514-66913533 None:intergenic 45.0%
TTCTTCATCAGACTGTGCCA+TGG + chr5.3:66912219-66912238 MS.gene46209:CDS 45.0%
! CTGAGGAAATCCGCTTCTTT+TGG + chr5.3:66914005-66914024 MS.gene46209:CDS 45.0%
! TTGATGGATAACTTGCGCGA+AGG - chr5.3:66913719-66913738 None:intergenic 45.0%
!! AAGCCTTGTTCGGTATTGTG+CGG - chr5.3:66913064-66913083 None:intergenic 45.0%
!! AAGTGCTGAACTCATCCAAG+GGG + chr5.3:66912714-66912733 MS.gene46209:CDS 45.0%
!! ATCTGTTGTGTGGGTGTTGA+TGG - chr5.3:66913636-66913655 None:intergenic 45.0%
!! CAGCTGTAAGGGTATTTCCT+TGG - chr5.3:66912952-66912971 None:intergenic 45.0%
!! CTTGTTTGGAAGCACTAGAG+TGG + chr5.3:66912435-66912454 MS.gene46209:CDS 45.0%
!! GAAGAGTGCTTTGAGTGCAA+GGG + chr5.3:66913386-66913405 MS.gene46209:CDS 45.0%
!! GGAGATGTTTTTGAGAGGTG+AGG + chr5.3:66912690-66912709 MS.gene46209:CDS 45.0%
!! GTAAGTGCTGAACTCATCCA+AGG + chr5.3:66912712-66912731 MS.gene46209:CDS 45.0%
!! TGAAGAGTGCTTTGAGTGCA+AGG + chr5.3:66913385-66913404 MS.gene46209:CDS 45.0%
!!! CACATGGTGTTTTCGAGTCA+TGG + chr5.3:66913031-66913050 MS.gene46209:CDS 45.0%
AAGGCGGAGAAAGTCCAAGA+TGG - chr5.3:66913890-66913909 None:intergenic 50.0%
ACTCGCAAGAATCCCCTTTC+TGG - chr5.3:66913008-66913027 None:intergenic 50.0%
AGACATCAGGCTCAGCATGT+TGG - chr5.3:66914067-66914086 None:intergenic 50.0%
ATATGCCTTCCTCTCCAGTG+AGG + chr5.3:66913742-66913761 MS.gene46209:CDS 50.0%
ATCCAGGTGAGAATGCAAGG+AGG + chr5.3:66912920-66912939 MS.gene46209:CDS 50.0%
ATCCCGCACAATACCGAACA+AGG + chr5.3:66913058-66913077 MS.gene46209:CDS 50.0%
ATGCTGAGCCTGATGTCTCT+TGG + chr5.3:66914069-66914088 MS.gene46209:CDS 50.0%
CAACACCTTCCTACGCGATT+TGG - chr5.3:66912671-66912690 None:intergenic 50.0%
CAAGGGCTTACTCTCACGAT+TGG + chr5.3:66912878-66912897 MS.gene46209:CDS 50.0%
CAAGGTGAAGCCATGCTCAA+GGG + chr5.3:66912861-66912880 MS.gene46209:CDS 50.0%
CACTCTTCAAACGGCTACCT+GGG - chr5.3:66913374-66913393 None:intergenic 50.0%
CGGAGTTTGTGGTTCCGATA+TGG + chr5.3:66912495-66912514 MS.gene46209:CDS 50.0%
CTCCCAAGAGTGGAAACATG+TGG + chr5.3:66913310-66913329 MS.gene46209:CDS 50.0%
CTGCCACATGTTTCCACTCT+TGG - chr5.3:66913316-66913335 None:intergenic 50.0%
GACATCAGGCTCAGCATGTT+GGG - chr5.3:66914066-66914085 None:intergenic 50.0%
GAGTGTCCCTTTGTTCATCC+AGG + chr5.3:66912904-66912923 MS.gene46209:CDS 50.0%
GGAGATGATGAACCTGCCAA+GGG - chr5.3:66913294-66913313 None:intergenic 50.0%
GGAGCAGATGTACAAAGAGC+AGG + chr5.3:66914205-66914224 MS.gene46209:CDS 50.0%
GGTTCATCATCTCCCAAGAG+TGG + chr5.3:66913300-66913319 MS.gene46209:CDS 50.0%
GTACAAAGAGCAGGAGCAGA+TGG + chr5.3:66914214-66914233 MS.gene46209:CDS 50.0%
GTCAAGCTCTTGCTTGATGC+CGG + chr5.3:66912571-66912590 MS.gene46209:CDS 50.0%
GTTGTCCAAATCGCGTAGGA+AGG + chr5.3:66912663-66912682 MS.gene46209:CDS 50.0%
TCAAGGTGAAGCCATGCTCA+AGG + chr5.3:66912860-66912879 MS.gene46209:CDS 50.0%
TCAGCATGTTGGGATGCAGT+TGG - chr5.3:66914056-66914075 None:intergenic 50.0%
TGAACTCATCCAAGGGGAGA+TGG + chr5.3:66912720-66912739 MS.gene46209:CDS 50.0%
TGAGAGTAAGCCCTTGAGCA+TGG - chr5.3:66912874-66912893 None:intergenic 50.0%
TGAGATGGAATTGCTCGGTC+TGG + chr5.3:66913419-66913438 MS.gene46209:CDS 50.0%
TGCTGAGCCTGATGTCTCTT+GGG + chr5.3:66914070-66914089 MS.gene46209:CDS 50.0%
TTCATCAGACTGTGCCATGG+AGG + chr5.3:66912222-66912241 MS.gene46209:CDS 50.0%
TTGTACATCTGCTCCACCCA+TGG - chr5.3:66914200-66914219 None:intergenic 50.0%
! AACCAGTGGAGGCATACACA+GGG - chr5.3:66913152-66913171 None:intergenic 50.0%
! ATGGATAACTTGCGCGAAGG+TGG - chr5.3:66913716-66913735 None:intergenic 50.0%
! CAAATCGCGTAGGAAGGTGT+TGG + chr5.3:66912669-66912688 MS.gene46209:CDS 50.0%
! CGCGAAGGTGGTTCATGTTT+TGG - chr5.3:66913704-66913723 None:intergenic 50.0%
! CTTCATTGTGCTGTTGCTGG+TGG + chr5.3:66912526-66912545 MS.gene46209:CDS 50.0%
! GCTCTTCATTGTGCTGTTGC+TGG + chr5.3:66912523-66912542 MS.gene46209:CDS 50.0%
! GGAGAGTGCATCTGTTGTGT+GGG - chr5.3:66913645-66913664 None:intergenic 50.0%
! TGAAGGCATAGCTGAACCAG+TGG - chr5.3:66913166-66913185 None:intergenic 50.0%
! TGGAGAGTGCATCTGTTGTG+TGG - chr5.3:66913646-66913665 None:intergenic 50.0%
!! AGCCTTGTTCGGTATTGTGC+GGG - chr5.3:66913063-66913082 None:intergenic 50.0%
!! CGTCCTTGCAAAGCCTTGTT+CGG - chr5.3:66913074-66913093 None:intergenic 50.0%
!! TGTGGGTGTTGATGGTTGCA+TGG - chr5.3:66913628-66913647 None:intergenic 50.0%
!!! TGGTTCTGAGTTTTCGCTCG+AGG + chr5.3:66912546-66912565 MS.gene46209:CDS 50.0%
AACACCACCCATGTCTCCCT+TGG + chr5.3:66913275-66913294 MS.gene46209:CDS 55.0%
ACCCCAATCGAGTTTCCCAG+TGG - chr5.3:66913961-66913980 None:intergenic 55.0%
ACGACCCCTTGCGAAACTCT+GGG - chr5.3:66912984-66913003 None:intergenic 55.0%
AGGCATAGCTGAACCAGTGG+AGG - chr5.3:66913163-66913182 None:intergenic 55.0%
AGGCTTTGCAAGGACGAGAC+TGG + chr5.3:66913078-66913097 MS.gene46209:CDS 55.0%
ATACACAGGGCGCAGCTCTT+CGG - chr5.3:66913139-66913158 None:intergenic 55.0%
ATGCAAGGAGGAGAGACCCA+AGG + chr5.3:66912932-66912951 MS.gene46209:CDS 55.0%
CATCACTGCAGCTGCTACTG+CGG - chr5.3:66913799-66913818 None:intergenic 55.0%
CCAATCGAGTTTCCCAGTGG+GGG - chr5.3:66913958-66913977 None:intergenic 55.0%
CCCAATCGAGTTTCCCAGTG+GGG - chr5.3:66913959-66913978 None:intergenic 55.0%
CCCACTGGGAAACTCGATTG+GGG + chr5.3:66913957-66913976 MS.gene46209:CDS 55.0%
CCCCAATCGAGTTTCCCAGT+GGG - chr5.3:66913960-66913979 None:intergenic 55.0%
CCCCACTGGGAAACTCGATT+GGG + chr5.3:66913956-66913975 MS.gene46209:CDS 55.0%
CTCCTCCTTGCATTCTCACC+TGG - chr5.3:66912925-66912944 None:intergenic 55.0%
CTGCAGCTGCCGCATATTCT+CGG - chr5.3:66914147-66914166 None:intergenic 55.0%
GAACCTGCCAAGGGAGACAT+GGG - chr5.3:66913285-66913304 None:intergenic 55.0%
GATGGTGAGTTGCAGCACCA+CGG - chr5.3:66913872-66913891 None:intergenic 55.0%
GCACTCTTCAAACGGCTACC+TGG - chr5.3:66913375-66913394 None:intergenic 55.0%
GCTGGCGCAATCAGATCAAC+CGG - chr5.3:66912634-66912653 None:intergenic 55.0%
GCTGTTACGGAAAGATGGGG+AGG - chr5.3:66913511-66913530 None:intergenic 55.0%
GGAACCCCAATCTGAAAGGC+CGG - chr5.3:66913937-66913956 None:intergenic 55.0%
GGCAGGGAACACAGCTGTAA+GGG - chr5.3:66912963-66912982 None:intergenic 55.0%
GGGAGATGATGAACCTGCCA+AGG - chr5.3:66913295-66913314 None:intergenic 55.0%
GTCGTCCGGCCTTTCAGATT+GGG + chr5.3:66913929-66913948 MS.gene46209:CDS 55.0%
GTGACGAGCTGAACAAGCTG+AGG + chr5.3:66913988-66914007 MS.gene46209:CDS 55.0%
GTGAGGAAGCCCTCACCATA+TGG + chr5.3:66913759-66913778 MS.gene46209:CDS 55.0%
TACATCTGCTCCACCCATGG+TGG - chr5.3:66914197-66914216 None:intergenic 55.0%
TATGGTGAGGGCTTCCTCAC+TGG - chr5.3:66913759-66913778 None:intergenic 55.0%
TCGAGACGTGCTTCCACCAT+GGG + chr5.3:66914181-66914200 MS.gene46209:CDS 55.0%
TCGTCCGGCCTTTCAGATTG+GGG + chr5.3:66913930-66913949 MS.gene46209:CDS 55.0%
TGAACCTGCCAAGGGAGACA+TGG - chr5.3:66913286-66913305 None:intergenic 55.0%
TGGCGCATCTGCATTGCACT+TGG - chr5.3:66913666-66913685 None:intergenic 55.0%
TGGGGGAACCCCAATCTGAA+AGG - chr5.3:66913941-66913960 None:intergenic 55.0%
TGTCGTCCGGCCTTTCAGAT+TGG + chr5.3:66913928-66913947 MS.gene46209:CDS 55.0%
! GAACCAGTGGAGGCATACAC+AGG - chr5.3:66913153-66913172 None:intergenic 55.0%
! GGTGCTGCAACTCACCATCT+TGG + chr5.3:66913873-66913892 MS.gene46209:CDS 55.0%
! TTGGCTGCGCTGCTTTGCAA+AGG - chr5.3:66913838-66913857 None:intergenic 55.0%
ACCCTGCAGGAAGGGTTGGA+AGG - chr5.3:66913909-66913928 None:intergenic 60.0%
AGACGTGCTTCCACCATGGG+TGG + chr5.3:66914184-66914203 MS.gene46209:CDS 60.0%
CAAGGGGTCGTGCCAGAAAG+GGG + chr5.3:66912993-66913012 MS.gene46209:CDS 60.0%
CACGACCCCTTGCGAAACTC+TGG - chr5.3:66912985-66913004 None:intergenic 60.0%
CAGATTGGGGTTCCCCCACT+GGG + chr5.3:66913943-66913962 MS.gene46209:CDS 60.0%
CCCCCACTGGGAAACTCGAT+TGG + chr5.3:66913955-66913974 MS.gene46209:CDS 60.0%
CCCTGCCCAGAGTTTCGCAA+GGG + chr5.3:66912976-66912995 MS.gene46209:CDS 60.0%
CCCTTGCGAAACTCTGGGCA+GGG - chr5.3:66912979-66912998 None:intergenic 60.0%
CCTGCCCAGAGTTTCGCAAG+GGG + chr5.3:66912977-66912996 MS.gene46209:CDS 60.0%
CTACCTGGGAGCTGCAACGA+TGG - chr5.3:66913360-66913379 None:intergenic 60.0%
CTGCAGGAAGGGTTGGAAGG+CGG - chr5.3:66913906-66913925 None:intergenic 60.0%
GCAAGGGGTCGTGCCAGAAA+GGG + chr5.3:66912992-66913011 MS.gene46209:CDS 60.0%
GCCTTCCAACCCTTCCTGCA+GGG + chr5.3:66913905-66913924 MS.gene46209:CDS 60.0%
GGCTTCCTCACTGGAGAGGA+AGG - chr5.3:66913750-66913769 None:intergenic 60.0%
GGGCAGGGAACACAGCTGTA+AGG - chr5.3:66912964-66912983 None:intergenic 60.0%
GTCGAGACGTGCTTCCACCA+TGG + chr5.3:66914180-66914199 MS.gene46209:CDS 60.0%
TACACAGGGCGCAGCTCTTC+GGG - chr5.3:66913138-66913157 None:intergenic 60.0%
TCAGATTGGGGTTCCCCCAC+TGG + chr5.3:66913942-66913961 MS.gene46209:CDS 60.0%
TCCCTGCCCAGAGTTTCGCA+AGG + chr5.3:66912975-66912994 MS.gene46209:CDS 60.0%
TGAGGGCTTCCTCACTGGAG+AGG - chr5.3:66913754-66913773 None:intergenic 60.0%
TGCTGCTGCTGTTGACGACG+AGG - chr5.3:66913450-66913469 None:intergenic 60.0%
!! AAGGAGCGTCCATCTCCCCT+TGG - chr5.3:66912732-66912751 None:intergenic 60.0%
!! GCTGCAACGATGGTGGAGTG+AGG - chr5.3:66913350-66913369 None:intergenic 60.0%
!! TGGGATGCAGTTGGTGCTGC+TGG - chr5.3:66914047-66914066 None:intergenic 60.0%
CCACCATCGTTGCAGCTCCC+AGG + chr5.3:66913354-66913373 MS.gene46209:CDS 65.0%
CCACCCATGTCTCCCTTGGC+AGG + chr5.3:66913279-66913298 MS.gene46209:CDS 65.0%
CCCCTTGCGAAACTCTGGGC+AGG - chr5.3:66912980-66912999 None:intergenic 65.0%
CCTGCCAAGGGAGACATGGG+TGG - chr5.3:66913282-66913301 None:intergenic 65.0%
CCTGGGAGCTGCAACGATGG+TGG - chr5.3:66913357-66913376 None:intergenic 65.0%
CGACACCCTGCAGGAAGGGT+TGG - chr5.3:66913913-66913932 None:intergenic 65.0%
CGCAAGGGGTCGTGCCAGAA+AGG + chr5.3:66912991-66913010 MS.gene46209:CDS 65.0%
CGCCCTGTGTATGCCTCCAC+TGG + chr5.3:66913147-66913166 MS.gene46209:CDS 65.0%
CGCCTTCCAACCCTTCCTGC+AGG + chr5.3:66913904-66913923 MS.gene46209:CDS 65.0%
CGGACGACACCCTGCAGGAA+GGG - chr5.3:66913917-66913936 None:intergenic 65.0%
!! CCTTCCTGCAGGGTGTCGTC+CGG + chr5.3:66913915-66913934 MS.gene46209:CDS 65.0%
AAGGCCGGACGACACCCTGC+AGG - chr5.3:66913922-66913941 None:intergenic 70.0%
CCGGACGACACCCTGCAGGA+AGG - chr5.3:66913918-66913937 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 66912193 66914244 66912193 ID=MS.gene46209
chr5.3 mRNA 66912193 66914244 66912193 ID=MS.gene46209.t1;Parent=MS.gene46209
chr5.3 exon 66912193 66914244 66912193 ID=MS.gene46209.t1.exon1;Parent=MS.gene46209.t1
chr5.3 CDS 66912193 66914244 66912193 ID=cds.MS.gene46209.t1;Parent=MS.gene46209.t1
Gene Sequence

>MS.gene46209

ATGTGCAGTGGTTCAAAGAGTAAACTTTCTTCATCAGACTGTGCCATGGAGGATAAGCTTCAAAAGGGTGTTCACAACTCCTCAATCTTGCTTGAATTGTCTGCATCTGATGATTTTGATGCTTTCAAGAGAGAAGTAGATGAGAAGGGTTTGGATGTGAATGAAGAAGGCTTTTGGTATGGTAGAAGAATTGGATCGAAGAAAATGGAATCCGAAAAGAGGACTCCTCTTATGATTGCTTCCTTGTTTGGAAGCACTAGAGTGGTCGAGTATATTCTTTCGACAGGGAAGATTGATGTCAACGGAGTTTGTGGTTCCGATATGGCCACTGCTCTTCATTGTGCTGTTGCTGGTGGTTCTGAGTTTTCGCTCGAGGTTGTCAAGCTCTTGCTTGATGCCGGTGCAGATGCTGATTGTCTTGATGCTAGTGGAAACAAACCGGTTGATCTGATTGCGCCAGCCTTTAATTCGTTGTCCAAATCGCGTAGGAAGGTGTTGGAGATGTTTTTGAGAGGTGAGGTAAGTGCTGAACTCATCCAAGGGGAGATGGACGCTCCTTTCTCATTGAAAAAAGAAGGTGGTGATAAGAAAGAGTTTCCTATTGATATATCGTTGCCCGACATAAACAACGGTGTATATGGATCAGATGAGTTTAGGATGTACAGTTTCAAGGTGAAGCCATGCTCAAGGGCTTACTCTCACGATTGGACCGAGTGTCCCTTTGTTCATCCAGGTGAGAATGCAAGGAGGAGAGACCCAAGGAAATACCCTTACAGCTGTGTTCCCTGCCCAGAGTTTCGCAAGGGGTCGTGCCAGAAAGGGGATTCTTGCGAGTATGCACATGGTGTTTTCGAGTCATGGCTACATCCCGCACAATACCGAACAAGGCTTTGCAAGGACGAGACTGGATGCAATAGAAAAGTCTGCTTCTTTGCTCACAGACCCGAAGAGCTGCGCCCTGTGTATGCCTCCACTGGTTCAGCTATGCCTTCACCAAAGTCCTATTCAGCTAGTGGAATTGACATGACATCGATGAGTCCGTTGTCTCTTAGCTCCTCATCTTTGCCTATGTCAACTGTTTCAACACCACCCATGTCTCCCTTGGCAGGTTCATCATCTCCCAAGAGTGGAAACATGTGGCAGAACAAATTAAACCTCACTCCACCATCGTTGCAGCTCCCAGGTAGCCGTTTGAAGAGTGCTTTGAGTGCAAGGGATCTCGATCTTGAGATGGAATTGCTCGGTCTGGATAGTCCTCGTCGTCAACAGCAGCAGCAACAACAACAACAATTGATTGAAGAGATTGCTAGAATCTCCTCCCCATCTTTCCGTAACAGCGAATTCAACAGAATTGCTGATTTGAATCCAACTAACCTTGATGACCTATTAGCATCAGCTGATCCTAATTTACGATCTCAACTACATGGACTGTCCATGCAACCATCAACACCCACACAACAGATGCACTCTCCAAGTGCAATGCAGATGCGCCAAAACATGAACATGGGCCAAAACATGAACCACCTTCGCGCAAGTTATCCATCAAACAATATGCCTTCCTCTCCAGTGAGGAAGCCCTCACCATATGGATTTGATTCATCAGCCGCAGTAGCAGCTGCAGTGATGAATTCTAGGTCTGCTGCCTTTGCAAAGCAGCGCAGCCAAAGTTTCATTGACCGTGGTGCTGCAACTCACCATCTTGGACTTTCTCCGCCTTCCAACCCTTCCTGCAGGGTGTCGTCCGGCCTTTCAGATTGGGGTTCCCCCACTGGGAAACTCGATTGGGGTGTAAACGGTGACGAGCTGAACAAGCTGAGGAAATCCGCTTCTTTTGGGTTCAGAAATAATGGACCAGCAGCACCAACTGCATCCCAACATGCTGAGCCTGATGTCTCTTGGGTTAATTCATTGGTTAAGGATGTTCCATCTGAGAATTCCGGTGTATATGGTGCCGAGAATATGCGGCAGCTGCAGCAATATGATCTGAGTCGAGACGTGCTTCCACCATGGGTGGAGCAGATGTACAAAGAGCAGGAGCAGATGGTAGCATGA
Protein sequence

>MS.gene46209.t1

MCSGSKSKLSSSDCAMEDKLQKGVHNSSILLELSASDDFDAFKREVDEKGLDVNEEGFWYGRRIGSKKMESEKRTPLMIASLFGSTRVVEYILSTGKIDVNGVCGSDMATALHCAVAGGSEFSLEVVKLLLDAGADADCLDASGNKPVDLIAPAFNSLSKSRRKVLEMFLRGEVSAELIQGEMDAPFSLKKEGGDKKEFPIDISLPDINNGVYGSDEFRMYSFKVKPCSRAYSHDWTECPFVHPGENARRRDPRKYPYSCVPCPEFRKGSCQKGDSCEYAHGVFESWLHPAQYRTRLCKDETGCNRKVCFFAHRPEELRPVYASTGSAMPSPKSYSASGIDMTSMSPLSLSSSSLPMSTVSTPPMSPLAGSSSPKSGNMWQNKLNLTPPSLQLPGSRLKSALSARDLDLEMELLGLDSPRRQQQQQQQQQLIEEIARISSPSFRNSEFNRIADLNPTNLDDLLASADPNLRSQLHGLSMQPSTPTQQMHSPSAMQMRQNMNMGQNMNHLRASYPSNNMPSSPVRKPSPYGFDSSAAVAAAVMNSRSAAFAKQRSQSFIDRGAATHHLGLSPPSNPSCRVSSGLSDWGSPTGKLDWGVNGDELNKLRKSASFGFRNNGPAAPTASQHAEPDVSWVNSLVKDVPSENSGVYGAENMRQLQQYDLSRDVLPPWVEQMYKEQEQMVA