AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53978

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53978.t1 MTR_5g071070 96.322 707 23 1 1 707 1 704 0.0 1420
MS.gene53978.t1 MTR_5g071070 96.800 625 17 1 83 707 1 622 0.0 1254
MS.gene53978.t1 MTR_5g082830 49.712 694 296 13 30 706 25 682 0.0 636
MS.gene53978.t1 MTR_1g075890 54.294 652 205 14 1 642 1 569 0.0 615
MS.gene53978.t1 MTR_7g092070 42.229 682 291 19 35 663 47 678 3.27e-155 467
MS.gene53978.t1 MTR_3g029590 39.532 726 343 18 33 702 43 728 3.18e-146 444
MS.gene53978.t1 MTR_3g464280 44.204 647 198 17 1 641 1 490 4.21e-143 428
MS.gene53978.t1 MTR_3g464260 48.583 494 167 12 39 529 29 438 8.26e-133 402
MS.gene53978.t1 MTR_3g464260 44.521 146 49 7 571 706 405 528 1.11e-17 87.4
MS.gene53978.t1 MTR_3g070880 46.404 431 161 5 33 462 8 369 3.19e-124 380
MS.gene53978.t1 MTR_3g070880 32.569 218 121 9 483 698 322 515 1.23e-17 87.0
MS.gene53978.t1 MTR_1g050438 40.406 542 255 15 35 538 15 526 4.79e-109 343
MS.gene53978.t1 MTR_0728s0020 40.406 542 255 15 35 538 31 542 1.44e-108 343
MS.gene53978.t1 MTR_3g435590 45.232 367 169 7 32 378 29 383 4.10e-97 308
MS.gene53978.t1 MTR_3g464290 40.733 491 184 18 199 675 68 465 1.50e-80 265
MS.gene53978.t1 MTR_3g435680 41.194 335 139 10 138 451 44 341 1.02e-72 241
MS.gene53978.t1 MTR_3g464270 38.532 436 177 14 229 660 8 356 1.61e-69 231
MS.gene53978.t1 MTR_8g102100 43.498 223 115 3 162 381 21 235 1.40e-54 192
MS.gene53978.t1 MTR_6g090380 57.971 138 48 2 196 332 77 205 6.21e-52 184
MS.gene53978.t1 MTR_3g103960 49.419 172 73 3 202 372 32 190 1.52e-51 181
MS.gene53978.t1 MTR_2g090605 61.261 111 43 0 230 340 77 187 2.86e-47 173
MS.gene53978.t1 MTR_4g078660 57.009 107 46 0 226 332 56 162 3.90e-44 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53978.t1 AT5G58620 47.050 695 267 18 23 706 2 606 0.0 572
MS.gene53978.t1 AT2G40140 42.297 714 285 17 1 706 1 595 9.42e-174 510
MS.gene53978.t1 AT2G40140 42.297 714 285 17 1 706 1 595 9.42e-174 510
MS.gene53978.t1 AT2G41900 39.702 738 300 23 35 702 48 710 5.58e-144 437
MS.gene53978.t1 AT3G55980 50.000 474 194 13 1 462 1 443 3.85e-142 428
MS.gene53978.t1 AT3G55980 38.071 197 82 8 514 706 419 579 2.48e-21 99.0
MS.gene53978.t1 AT3G55980 50.000 474 194 13 1 462 68 510 1.20e-141 429
MS.gene53978.t1 AT3G55980 38.071 197 82 8 514 706 486 646 4.27e-21 98.6
MS.gene53978.t1 AT5G12850 38.267 750 306 25 30 702 37 706 1.34e-139 426
MS.gene53978.t1 AT4G29190 52.273 176 83 1 212 387 62 236 1.72e-54 191
MS.gene53978.t1 AT2G25900 66.964 112 37 0 226 337 86 197 3.50e-53 186
MS.gene53978.t1 AT2G25900 66.964 112 37 0 226 337 86 197 3.50e-53 186
MS.gene53978.t1 AT2G19810 63.333 120 44 0 215 334 63 182 8.35e-53 186
MS.gene53978.t1 AT5G07500 52.703 148 66 1 189 332 7 154 1.26e-49 174
MS.gene53978.t1 AT1G03790 51.634 153 66 3 197 345 53 201 2.67e-47 172
MS.gene53978.t1 AT5G44260 55.970 134 54 2 210 342 44 173 2.67e-45 166

Find 192 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGTCTTCAACCTGCTTT+TGG 0.190182 5.2:-60111135 None:intergenic
TCAGACATGTCTTCTGTTTC+CGG 0.194365 5.2:+60110455 MS.gene53978:CDS
GATGTATCGGCTTATATTCT+TGG 0.209122 5.2:+60110183 MS.gene53978:CDS
CTCTTCATTGTGCGGTTGTT+GGG 0.225881 5.2:+60110256 MS.gene53978:CDS
AAAGTTGGTTCAAACAAATT+AGG 0.238453 5.2:+60110111 MS.gene53978:CDS
GGGCTCACCCGATGGAAATT+TGG 0.238746 5.2:+60111718 MS.gene53978:CDS
CTAGCTGCAGCTGGAGTTAA+AGG 0.264190 5.2:-60111815 None:intergenic
GAGGATGCTACAAGCTCTTT+TGG 0.287126 5.2:+60110413 MS.gene53978:CDS
ATACGACCGGGGAACTAAAT+AGG 0.321029 5.2:+60111321 MS.gene53978:CDS
GCGTCCCTTGTCCTGAGTTT+CGG 0.326990 5.2:+60110727 MS.gene53978:CDS
CTGAGGAAATCATCTTCATT+TGG 0.338854 5.2:+60111773 MS.gene53978:CDS
GCTCTTCATTGTGCGGTTGT+TGG 0.359015 5.2:+60110255 MS.gene53978:CDS
GATTCATATTCGGACGCATT+TGG 0.363999 5.2:-60111478 None:intergenic
ACCTATAGGTTTGATCCATC+TGG 0.364237 5.2:+60111560 MS.gene53978:CDS
TGCAGATGCTGATTCATATT+CGG 0.365840 5.2:-60111488 None:intergenic
ACGCCGCCTTTGACTTCATC+TGG 0.368423 5.2:+60111038 MS.gene53978:CDS
GTCTTTCCTCTCCTTCCAAC+TGG 0.371979 5.2:+60111249 MS.gene53978:CDS
TATATAGTACCGATGAGTTT+AGG 0.375544 5.2:+60110583 MS.gene53978:CDS
TCCTGTGCCCTAGCTGCAGC+TGG 0.377666 5.2:-60111824 None:intergenic
CCTTGGATTGAAGATAGAAT+TGG 0.386827 5.2:-60110388 None:intergenic
CTGCAACAAAACTATTCTTC+AGG 0.389322 5.2:+60111506 MS.gene53978:CDS
CGCCCCTTGTATGCTTCTAC+CGG 0.390009 5.2:+60110903 MS.gene53978:CDS
GATGGGGTTGGTTTGTGGTA+TGG 0.391400 5.2:+60110084 MS.gene53978:CDS
AGCAAAATCTTGATAATCTC+GGG 0.396619 5.2:-60110294 None:intergenic
CATTCCACTTCTAGTGTGAT+TGG 0.397045 5.2:+60111530 MS.gene53978:CDS
CACATGGCTGCAGCAGCAAC+GGG 0.402327 5.2:-60111071 None:intergenic
ATTCAGATATTGCAATGCTT+AGG 0.408619 5.2:+60111177 MS.gene53978:CDS
AAAATGTCTTCAAGGTTTGT+AGG 0.409953 5.2:-60111359 None:intergenic
CAACGGAACAGATTCGATTC+CGG 0.410373 5.2:+60111964 MS.gene53978:CDS
TGGAATTTATGCAACATTGA+TGG 0.416189 5.2:-60111395 None:intergenic
AATCTGTTCCGTTGTTAAGA+TGG 0.416969 5.2:-60111955 None:intergenic
TTCAGATATTGCAATGCTTA+GGG 0.419562 5.2:+60111178 MS.gene53978:CDS
TCGGCTTATATTCTTGGAAC+AGG 0.420142 5.2:+60110189 MS.gene53978:CDS
TTGTCAAAAGGATTTGATCA+TGG 0.420181 5.2:+60109936 MS.gene53978:CDS
TGATTGGATCGCCAACCTAT+AGG 0.421260 5.2:+60111546 MS.gene53978:CDS
TATATCCCATTCTTAATGTC+TGG 0.426530 5.2:-60110564 None:intergenic
CTCATGGTTGCAGCTATGTA+TGG 0.426552 5.2:+60110153 MS.gene53978:CDS
GCTTGTAGCATCCTCTTCCT+TGG 0.429452 5.2:-60110405 None:intergenic
GAAAAGGCATAAGGCTTAGC+AGG 0.430069 5.2:-60111689 None:intergenic
AAGCAAAATCTTGATAATCT+CGG 0.433411 5.2:-60110295 None:intergenic
CTTGATGCTGCAGGAGCAGC+AGG 0.434896 5.2:+60111428 MS.gene53978:CDS
GAGACCCGAAAATGTCTTCA+AGG 0.438096 5.2:-60111367 None:intergenic
CTTATATTCTTGGAACAGGT+CGG 0.443594 5.2:+60110193 MS.gene53978:CDS
ATGTTGATGGGGTTGGTTTG+TGG 0.450945 5.2:+60110079 MS.gene53978:CDS
GTATGGTTGGTCGAAGAAAC+CGG 0.454341 5.2:-60111302 None:intergenic
GAGTGTCCCTTTGTTCATCC+AGG 0.455762 5.2:+60110660 MS.gene53978:CDS
GGTTGCGATGTTGATGGGGT+TGG 0.455769 5.2:+60110072 MS.gene53978:CDS
TCAAAGTGAAGCCTTGTTCA+AGG 0.457380 5.2:+60110616 MS.gene53978:CDS
GCAGTACTGTAATATGATCT+AGG 0.457857 5.2:-60110942 None:intergenic
CACCCGATGGAAATTTGGAT+TGG 0.458700 5.2:+60111723 MS.gene53978:CDS
AGCGAAACAATCAAATCAAC+TGG 0.459199 5.2:-60110363 None:intergenic
AGTTGATTTGATTGTTTCGC+TGG 0.460352 5.2:+60110365 MS.gene53978:CDS
CCACATGGCTGCAGCAGCAA+CGG 0.462166 5.2:-60111072 None:intergenic
TGTCAAAAGGATTTGATCAT+GGG 0.468107 5.2:+60109937 MS.gene53978:CDS
CTTCTTTGCTCACAAGGTCG+AGG 0.469734 5.2:+60110875 MS.gene53978:CDS
ACGAGGGTTGCGATGTTGAT+GGG 0.471244 5.2:+60110067 MS.gene53978:CDS
TTGGGTTAATTCGCTTGTAA+AGG 0.474557 5.2:+60111871 MS.gene53978:CDS
TCTCTTCCAGACATTAAGAA+TGG 0.478120 5.2:+60110558 MS.gene53978:CDS
AGAATTTCACTTGATGCTGC+AGG 0.479378 5.2:+60111419 MS.gene53978:CDS
CTTTAACTCCAGCTGCAGCT+AGG 0.479692 5.2:+60111816 MS.gene53978:CDS
GGAGCAATTGTACATGGATC+AGG 0.480663 5.2:+60111994 MS.gene53978:CDS
CCCTTCAGCTTCCCAAAAGC+AGG 0.481050 5.2:+60111123 MS.gene53978:CDS
CTCTTCCAGACATTAAGAAT+GGG 0.482849 5.2:+60110559 MS.gene53978:CDS
GTCGAAGAAACCGGAAAAGA+TGG 0.483591 5.2:-60111293 None:intergenic
TACTGCTTCTACATTAGAGA+TGG 0.486960 5.2:+60110959 MS.gene53978:CDS
TATGTGAATGGGAATAAAGA+AGG 0.487426 5.2:+60109967 MS.gene53978:CDS
CGGAAAGGATCGTGCAGTAA+AGG 0.487429 5.2:+60110747 MS.gene53978:CDS
AGATTCGATTCCGGCTTGGT+TGG 0.488203 5.2:+60111973 MS.gene53978:CDS
ACCGGAAAAGATGGACTGTT+AGG 0.489839 5.2:-60111284 None:intergenic
AGCTGGAGTTAAAGGAGTAC+TGG 0.489995 5.2:-60111807 None:intergenic
TTATATTCTTGGAACAGGTC+GGG 0.491005 5.2:+60110194 MS.gene53978:CDS
TCTTCATTGTGCGGTTGTTG+GGG 0.492583 5.2:+60110257 MS.gene53978:CDS
TTGTGGTATGGAAGGAAAGT+TGG 0.493676 5.2:+60110096 MS.gene53978:CDS
ATGGGGTGTGAGTATGTGAA+TGG 0.494445 5.2:+60109955 MS.gene53978:CDS
CAGGTCGGGTTGATGTTAAT+CGG 0.495738 5.2:+60110208 MS.gene53978:CDS
GTATGGAAGCTTGGATGTAT+CGG 0.499389 5.2:+60110170 MS.gene53978:CDS
CACTGCGCTCGATAAAGCTC+TGG 0.499905 5.2:-60111637 None:intergenic
TCTGGAAGAGAGACATCAAC+AGG 0.501027 5.2:-60110546 None:intergenic
CCCAGATGGATCAAACCTAT+AGG 0.501454 5.2:-60111561 None:intergenic
TTTAACTCCAGCTGCAGCTA+GGG 0.502176 5.2:+60111817 MS.gene53978:CDS
TATTTAGTTCCCCGGTCGTA+TGG 0.502935 5.2:-60111319 None:intergenic
AGGAGATGGAATAGCAGAAC+CGG 0.503649 5.2:-60110922 None:intergenic
TCTCAGTTACAATCTCCAAC+TGG 0.504676 5.2:+60111452 MS.gene53978:CDS
ATGGAGAACCAAGTGACATT+GGG 0.506623 5.2:-60110992 None:intergenic
GGACGCATTTGGATTCCAGT+TGG 0.507506 5.2:-60111467 None:intergenic
CCTATAGGTTTGATCCATCT+GGG 0.508102 5.2:+60111561 MS.gene53978:CDS
CTATGAACCAGATGTGTCTT+GGG 0.508226 5.2:+60111853 MS.gene53978:CDS
TCCAGCTGCAGCTAGGGCAC+AGG 0.510410 5.2:+60111823 MS.gene53978:CDS
CCCAAAAGCAGGTTGAAGAC+TGG 0.511273 5.2:+60111134 MS.gene53978:CDS
CAAGGGCATATTCTCATGAC+TGG 0.511439 5.2:+60110634 MS.gene53978:CDS
ACACGGCTTTGCAAGGATGA+AGG 0.512844 5.2:+60110831 MS.gene53978:CDS
TGCAGCTATGTATGGAAGCT+TGG 0.514902 5.2:+60110161 MS.gene53978:CDS
GAATTAACCCAAGACACATC+TGG 0.514995 5.2:-60111860 None:intergenic
TAGTTCCCCGGTCGTATGGT+TGG 0.517395 5.2:-60111315 None:intergenic
TTAGCGTTGAAGATCAGAAA+AGG 0.521469 5.2:+60111921 MS.gene53978:CDS
GATGGAGAACCAAGTGACAT+TGG 0.523747 5.2:-60110993 None:intergenic
AACGAGGGTTGCGATGTTGA+TGG 0.524302 5.2:+60110066 MS.gene53978:CDS
CGAAAACCTATTTAGTTCCC+CGG 0.524607 5.2:-60111327 None:intergenic
GCTCTGGCTCCGCTTTGAAA+AGG 0.525597 5.2:-60111621 None:intergenic
AGGGGAAAATGCAAGGCGAC+GGG 0.532175 5.2:+60110680 MS.gene53978:CDS
GAACAGATTCGATTCCGGCT+TGG 0.533268 5.2:+60111969 MS.gene53978:CDS
TCAATTAGCCCAATGTCACT+TGG 0.534086 5.2:+60110984 MS.gene53978:CDS
CCCCAGTTAGAAAAGGCATA+AGG 0.538596 5.2:-60111698 None:intergenic
GTCCAATCCAAATTTCCATC+GGG 0.538779 5.2:-60111725 None:intergenic
TTGGTTGGAGCAATTGTACA+TGG 0.540592 5.2:+60111988 MS.gene53978:CDS
TCTTCGACCAACCATACGAC+CGG 0.541735 5.2:+60111308 MS.gene53978:CDS
CGATCCAATCACACTAGAAG+TGG 0.542472 5.2:-60111534 None:intergenic
GGGTGAGCCCCAGTTAGAAA+AGG 0.542739 5.2:-60111705 None:intergenic
ACTATGAACCAGATGTGTCT+TGG 0.542920 5.2:+60111852 MS.gene53978:CDS
ACTGCTTCTACATTAGAGAT+GGG 0.544371 5.2:+60110960 MS.gene53978:CDS
TACAATTGCTCCAACCAAGC+CGG 0.546247 5.2:-60111983 None:intergenic
GTCAAAGGCGGCGTTGAGGA+GGG 0.547086 5.2:-60111029 None:intergenic
CTATCTTCAATCCAAGGAAG+AGG 0.548194 5.2:+60110394 MS.gene53978:CDS
AGTTTGCTTCTTTGCTCACA+AGG 0.549883 5.2:+60110869 MS.gene53978:CDS
CACGATCCTTTCCGAAACTC+AGG 0.550059 5.2:-60110738 None:intergenic
CAAAGTGAAGCCTTGTTCAA+GGG 0.551738 5.2:+60110617 MS.gene53978:CDS
GCGAAAGCAGCAACTACTGA+TGG 0.552696 5.2:+60111214 MS.gene53978:CDS
CTTCGACCAACCATACGACC+GGG 0.555663 5.2:+60111309 MS.gene53978:CDS
CCAATTCTATCTTCAATCCA+AGG 0.560551 5.2:+60110388 MS.gene53978:CDS
AGCCGTGTCTTATACTGCGC+AGG 0.561388 5.2:-60110816 None:intergenic
CTGTAATATGATCTAGGAGA+TGG 0.561399 5.2:-60110936 None:intergenic
AGTCAAAGGCGGCGTTGAGG+AGG 0.561805 5.2:-60111030 None:intergenic
TCAATGTCATCAATTGTCTC+CGG 0.563566 5.2:-60110474 None:intergenic
TTCATCCAGGGGAAAATGCA+AGG 0.563820 5.2:+60110673 MS.gene53978:CDS
AGAACCGGTAGAAGCATACA+AGG 0.565045 5.2:-60110907 None:intergenic
CTACTGATGGATGAGATGTC+CGG 0.565792 5.2:+60111227 MS.gene53978:CDS
CAGGGGAAAATGCAAGGCGA+CGG 0.567408 5.2:+60110679 MS.gene53978:CDS
GGGTTGGTTTGTGGTATGGA+AGG 0.567780 5.2:+60110088 MS.gene53978:CDS
GTTGATGTTAATCGGTCTAG+TGG 0.567818 5.2:+60110216 MS.gene53978:CDS
ACAAATTAGGTTATGAAGAG+AGG 0.569699 5.2:+60110124 MS.gene53978:CDS
TCTAACTGGGGCTCACCCGA+TGG 0.570083 5.2:+60111710 MS.gene53978:CDS
TCAGCTGCAGATGATGTGGT+TGG 0.573637 5.2:+60110024 MS.gene53978:CDS
GAGGGTGGTATCAAAACAGA+TGG 0.574942 5.2:-60111011 None:intergenic
TGAAGAGAGGACACCTCTCA+TGG 0.576333 5.2:+60110137 MS.gene53978:CDS
AGTGTCCCTTTGTTCATCCA+GGG 0.576667 5.2:+60110661 MS.gene53978:CDS
ATGGAATGATTCCAGTTGGA+AGG 0.579291 5.2:-60111260 None:intergenic
CCTTTCCGAAACTCAGGACA+AGG 0.580350 5.2:-60110732 None:intergenic
GTATAAGACACGGCTTTGCA+AGG 0.581068 5.2:+60110824 MS.gene53978:CDS
GTTGGAAGGAGAGGAAAGAC+CGG 0.583195 5.2:-60111246 None:intergenic
ATGATTCCAGTTGGAAGGAG+AGG 0.583775 5.2:-60111255 None:intergenic
GATGGATCAAACCTATAGGT+TGG 0.584232 5.2:-60111557 None:intergenic
AGTCCAATCCAAATTTCCAT+CGG 0.586204 5.2:-60111726 None:intergenic
ATGAAGAGCAGTAGCACCAT+CGG 0.586647 5.2:-60110241 None:intergenic
ATGTGAATGGGAATAAAGAA+GGG 0.588204 5.2:+60109968 MS.gene53978:CDS
GACGCGGATAACTCCCCAGA+TGG 0.588308 5.2:-60111575 None:intergenic
AGGCATGGAATGATTCCAGT+TGG 0.590112 5.2:-60111264 None:intergenic
CCTTGTCCTGAGTTTCGGAA+AGG 0.592498 5.2:+60110732 MS.gene53978:CDS
CCGTTGCTGCTGCAGCCATG+TGG 0.592506 5.2:+60111072 MS.gene53978:CDS
TGAGAATATGCCCTTGAACA+AGG 0.592999 5.2:-60110627 None:intergenic
CAATCACACTAGAAGTGGAA+TGG 0.595527 5.2:-60111529 None:intergenic
ATCAACTGGTCGGTTACCGT+TGG 0.595630 5.2:-60110349 None:intergenic
ATCTGAATTAATATCTCTAG+CGG 0.595917 5.2:-60111162 None:intergenic
CGTATACATCCTAAACTCAT+CGG 0.599276 5.2:-60110592 None:intergenic
TGGGGTGTGAGTATGTGAAT+GGG 0.600624 5.2:+60109956 MS.gene53978:CDS
ATGACATTGACGAATATCAA+AGG 0.602422 5.2:+60110487 MS.gene53978:CDS
ATCTGGGGAGTTATCCGCGT+CGG 0.603525 5.2:+60111577 MS.gene53978:CDS
AATTTGGATTGGACTTCACA+CGG 0.603583 5.2:+60111734 MS.gene53978:CDS
CAGCATCAAGTGAAATTCTG+TGG 0.605687 5.2:-60111415 None:intergenic
TGCTACTGCTCTTCATTGTG+CGG 0.605703 5.2:+60110248 MS.gene53978:CDS
GAGAATGAAGCTCAGAATGA+CGG 0.613447 5.2:-60111667 None:intergenic
AAAAGATGGACTGTTAGGCA+TGG 0.613610 5.2:-60111279 None:intergenic
TGGATGAACAAAGGGACACT+CGG 0.617153 5.2:-60110658 None:intergenic
AATCGGTCTAGTGGATCCGA+TGG 0.617627 5.2:+60110225 MS.gene53978:CDS
TACATAGCTGCAACCATGAG+AGG 0.624576 5.2:-60110150 None:intergenic
GCAAAATCTTGATAATCTCG+GGG 0.625212 5.2:-60110293 None:intergenic
GTCAAAAGGATTTGATCATG+GGG 0.630254 5.2:+60109938 MS.gene53978:CDS
GTGTCCCTTTGTTCATCCAG+GGG 0.633464 5.2:+60110662 MS.gene53978:CDS
CGAGGGTTGCGATGTTGATG+GGG 0.634282 5.2:+60110068 MS.gene53978:CDS
CTTCATTGTGCGGTTGTTGG+GGG 0.635961 5.2:+60110258 MS.gene53978:CDS
GATGCTCCAGATGAAGTCAA+AGG 0.639371 5.2:-60111044 None:intergenic
AAACAATCAAATCAACTGGT+CGG 0.641933 5.2:-60110359 None:intergenic
CTAAACTGATGAGATTCTTG+CGG 0.647291 5.2:-60111902 None:intergenic
AAAGGCGGCGTTGAGGAGGG+TGG 0.647882 5.2:-60111026 None:intergenic
GCTAGTGCTGTTGATGCCAA+CGG 0.653244 5.2:+60110333 MS.gene53978:CDS
ATTAGACGGTGTCTGCCACA+TGG 0.653248 5.2:-60111087 None:intergenic
TAAAGAAGGGCAGTATCATG+AGG 0.655064 5.2:+60109981 MS.gene53978:CDS
TGGAGTTAAAGGAGTACTGG+TGG 0.655843 5.2:-60111804 None:intergenic
TGAAGTCAAAGGCGGCGTTG+AGG 0.657176 5.2:-60111033 None:intergenic
GAGAAGAACTGAATAAGCTG+AGG 0.666361 5.2:+60111756 MS.gene53978:CDS
TTCGACCAACCATACGACCG+GGG 0.672912 5.2:+60111310 MS.gene53978:CDS
TGCTTAGGGTTGAAACTCAG+CGG 0.673294 5.2:+60111192 MS.gene53978:CDS
AACCGGTAGAAGCATACAAG+GGG 0.673738 5.2:-60110905 None:intergenic
GAACCGGTAGAAGCATACAA+GGG 0.683674 5.2:-60110906 None:intergenic
CTGCAGCGCCATCTTAACAA+CGG 0.687526 5.2:+60111947 MS.gene53978:CDS
CTATAGGTTTGATCCATCTG+GGG 0.687753 5.2:+60111562 MS.gene53978:CDS
ATCCTGCGCAGTATAAGACA+CGG 0.689588 5.2:+60110814 MS.gene53978:CDS
CAAAATCTTGATAATCTCGG+GGG 0.705772 5.2:-60110292 None:intergenic
TCTTGCATTCAAAGCCGACG+CGG 0.707580 5.2:-60111591 None:intergenic
GATTCTTGTGACTATGCACA+CGG 0.716065 5.2:+60110771 MS.gene53978:CDS
CTTTCCGAAACTCAGGACAA+GGG 0.724035 5.2:-60110731 None:intergenic
GTAGGGATCGAAACATTAGA+CGG 0.728881 5.2:-60111101 None:intergenic
GCTCCAGATGAAGTCAAAGG+CGG 0.734283 5.2:-60111041 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAAAAAACTTGTCAAA+AGG + chr5.2:60109924-60109943 MS.gene53978:CDS 20.0%
! ATCTGAATTAATATCTCTAG+CGG - chr5.2:60111165-60111184 None:intergenic 25.0%
!! AAAGTTGGTTCAAACAAATT+AGG + chr5.2:60110111-60110130 MS.gene53978:CDS 25.0%
!! AAGCAAAATCTTGATAATCT+CGG - chr5.2:60110298-60110317 None:intergenic 25.0%
!! CAGCTATAATGATATTTTCT+TGG - chr5.2:60110708-60110727 None:intergenic 25.0%
AAAAAATGCGATTGAAAACG+AGG + chr5.2:60110050-60110069 MS.gene53978:CDS 30.0%
AAAAATGCGATTGAAAACGA+GGG + chr5.2:60110051-60110070 MS.gene53978:CDS 30.0%
AAAATGTCTTCAAGGTTTGT+AGG - chr5.2:60111362-60111381 None:intergenic 30.0%
AAACAATCAAATCAACTGGT+CGG - chr5.2:60110362-60110381 None:intergenic 30.0%
ACAAATTAGGTTATGAAGAG+AGG + chr5.2:60110124-60110143 MS.gene53978:CDS 30.0%
ATGACATTGACGAATATCAA+AGG + chr5.2:60110487-60110506 MS.gene53978:CDS 30.0%
ATGTGAATGGGAATAAAGAA+GGG + chr5.2:60109968-60109987 MS.gene53978:CDS 30.0%
ATTCAGATATTGCAATGCTT+AGG + chr5.2:60111177-60111196 MS.gene53978:CDS 30.0%
TATATCCCATTCTTAATGTC+TGG - chr5.2:60110567-60110586 None:intergenic 30.0%
TATGTGAATGGGAATAAAGA+AGG + chr5.2:60109967-60109986 MS.gene53978:CDS 30.0%
TGGAATTTATGCAACATTGA+TGG - chr5.2:60111398-60111417 None:intergenic 30.0%
TGTCAAAAGGATTTGATCAT+GGG + chr5.2:60109937-60109956 MS.gene53978:CDS 30.0%
TTCAGATATTGCAATGCTTA+GGG + chr5.2:60111178-60111197 MS.gene53978:CDS 30.0%
TTGTCAAAAGGATTTGATCA+TGG + chr5.2:60109936-60109955 MS.gene53978:CDS 30.0%
! ACAAACCTTGAAGACATTTT+CGG + chr5.2:60111362-60111381 MS.gene53978:CDS 30.0%
! AGCAAAATCTTGATAATCTC+GGG - chr5.2:60110297-60110316 None:intergenic 30.0%
!! TATATAGTACCGATGAGTTT+AGG + chr5.2:60110583-60110602 MS.gene53978:CDS 30.0%
AATCTGTTCCGTTGTTAAGA+TGG - chr5.2:60111958-60111977 None:intergenic 35.0%
ACTGCTTCTACATTAGAGAT+GGG + chr5.2:60110960-60110979 MS.gene53978:CDS 35.0%
AGCGAAACAATCAAATCAAC+TGG - chr5.2:60110366-60110385 None:intergenic 35.0%
AGTCCAATCCAAATTTCCAT+CGG - chr5.2:60111729-60111748 None:intergenic 35.0%
CCAATTCTATCTTCAATCCA+AGG + chr5.2:60110388-60110407 MS.gene53978:CDS 35.0%
CCTTGGATTGAAGATAGAAT+TGG - chr5.2:60110391-60110410 None:intergenic 35.0%
CGTATACATCCTAAACTCAT+CGG - chr5.2:60110595-60110614 None:intergenic 35.0%
CTAAACTGATGAGATTCTTG+CGG - chr5.2:60111905-60111924 None:intergenic 35.0%
CTCTTCCAGACATTAAGAAT+GGG + chr5.2:60110559-60110578 MS.gene53978:CDS 35.0%
CTGAGGAAATCATCTTCATT+TGG + chr5.2:60111773-60111792 MS.gene53978:CDS 35.0%
CTGCAACAAAACTATTCTTC+AGG + chr5.2:60111506-60111525 MS.gene53978:CDS 35.0%
CTGTAATATGATCTAGGAGA+TGG - chr5.2:60110939-60110958 None:intergenic 35.0%
CTTATATTCTTGGAACAGGT+CGG + chr5.2:60110193-60110212 MS.gene53978:CDS 35.0%
GCAGTACTGTAATATGATCT+AGG - chr5.2:60110945-60110964 None:intergenic 35.0%
GTCAAAAGGATTTGATCATG+GGG + chr5.2:60109938-60109957 MS.gene53978:CDS 35.0%
TACTGCTTCTACATTAGAGA+TGG + chr5.2:60110959-60110978 MS.gene53978:CDS 35.0%
TCAATGTCATCAATTGTCTC+CGG - chr5.2:60110477-60110496 None:intergenic 35.0%
TCTCTTCCAGACATTAAGAA+TGG + chr5.2:60110558-60110577 MS.gene53978:CDS 35.0%
TTATATTCTTGGAACAGGTC+GGG + chr5.2:60110194-60110213 MS.gene53978:CDS 35.0%
TTGGGTTAATTCGCTTGTAA+AGG + chr5.2:60111871-60111890 MS.gene53978:CDS 35.0%
! AATTTGGATTGGACTTCACA+CGG + chr5.2:60111734-60111753 MS.gene53978:CDS 35.0%
! CAAAATCTTGATAATCTCGG+GGG - chr5.2:60110295-60110314 None:intergenic 35.0%
! CAAACCTTGAAGACATTTTC+GGG + chr5.2:60111363-60111382 MS.gene53978:CDS 35.0%
! CAAGATTTTGCTTGATGCAT+CGG + chr5.2:60110305-60110324 MS.gene53978:CDS 35.0%
! GCAAAATCTTGATAATCTCG+GGG - chr5.2:60110296-60110315 None:intergenic 35.0%
! TTAGCGTTGAAGATCAGAAA+AGG + chr5.2:60111921-60111940 MS.gene53978:CDS 35.0%
!! AGTTGATTTGATTGTTTCGC+TGG + chr5.2:60110365-60110384 MS.gene53978:CDS 35.0%
!! GATGTATCGGCTTATATTCT+TGG + chr5.2:60110183-60110202 MS.gene53978:CDS 35.0%
!! TGCAGATGCTGATTCATATT+CGG - chr5.2:60111491-60111510 None:intergenic 35.0%
!!! GGATAATCTTTTGAAACACG+AGG - chr5.2:60110528-60110547 None:intergenic 35.0%
ACCTATAGGTTTGATCCATC+TGG + chr5.2:60111560-60111579 MS.gene53978:CDS 40.0%
ACTATGAACCAGATGTGTCT+TGG + chr5.2:60111852-60111871 MS.gene53978:CDS 40.0%
AGAATTTCACTTGATGCTGC+AGG + chr5.2:60111419-60111438 MS.gene53978:CDS 40.0%
ATGGAATGATTCCAGTTGGA+AGG - chr5.2:60111263-60111282 None:intergenic 40.0%
ATGGAGAACCAAGTGACATT+GGG - chr5.2:60110995-60111014 None:intergenic 40.0%
CAATCACACTAGAAGTGGAA+TGG - chr5.2:60111532-60111551 None:intergenic 40.0%
CAGCATCAAGTGAAATTCTG+TGG - chr5.2:60111418-60111437 None:intergenic 40.0%
CATTCCACTTCTAGTGTGAT+TGG + chr5.2:60111530-60111549 MS.gene53978:CDS 40.0%
CCTATAGGTTTGATCCATCT+GGG + chr5.2:60111561-60111580 MS.gene53978:CDS 40.0%
CTATAGGTTTGATCCATCTG+GGG + chr5.2:60111562-60111581 MS.gene53978:CDS 40.0%
CTATCTTCAATCCAAGGAAG+AGG + chr5.2:60110394-60110413 MS.gene53978:CDS 40.0%
CTATGAACCAGATGTGTCTT+GGG + chr5.2:60111853-60111872 MS.gene53978:CDS 40.0%
GAATTAACCCAAGACACATC+TGG - chr5.2:60111863-60111882 None:intergenic 40.0%
GAGAAGAACTGAATAAGCTG+AGG + chr5.2:60111756-60111775 MS.gene53978:CDS 40.0%
GAGAATGAAGCTCAGAATGA+CGG - chr5.2:60111670-60111689 None:intergenic 40.0%
GATGGATCAAACCTATAGGT+TGG - chr5.2:60111560-60111579 None:intergenic 40.0%
GATTCATATTCGGACGCATT+TGG - chr5.2:60111481-60111500 None:intergenic 40.0%
GATTCTTGTGACTATGCACA+CGG + chr5.2:60110771-60110790 MS.gene53978:CDS 40.0%
GTAGGGATCGAAACATTAGA+CGG - chr5.2:60111104-60111123 None:intergenic 40.0%
GTATGGAAGCTTGGATGTAT+CGG + chr5.2:60110170-60110189 MS.gene53978:CDS 40.0%
GTCCAATCCAAATTTCCATC+GGG - chr5.2:60111728-60111747 None:intergenic 40.0%
GTTGATGTTAATCGGTCTAG+TGG + chr5.2:60110216-60110235 MS.gene53978:CDS 40.0%
TAAAGAAGGGCAGTATCATG+AGG + chr5.2:60109981-60110000 MS.gene53978:CDS 40.0%
TCAATTAGCCCAATGTCACT+TGG + chr5.2:60110984-60111003 MS.gene53978:CDS 40.0%
TCAGACATGTCTTCTGTTTC+CGG + chr5.2:60110455-60110474 MS.gene53978:CDS 40.0%
TCGGCTTATATTCTTGGAAC+AGG + chr5.2:60110189-60110208 MS.gene53978:CDS 40.0%
TCTCAGTTACAATCTCCAAC+TGG + chr5.2:60111452-60111471 MS.gene53978:CDS 40.0%
TGAGAATATGCCCTTGAACA+AGG - chr5.2:60110630-60110649 None:intergenic 40.0%
TTGGTTGGAGCAATTGTACA+TGG + chr5.2:60111988-60112007 MS.gene53978:CDS 40.0%
TTGTGGTATGGAAGGAAAGT+TGG + chr5.2:60110096-60110115 MS.gene53978:CDS 40.0%
! AGTTTGCTTCTTTGCTCACA+AGG + chr5.2:60110869-60110888 MS.gene53978:CDS 40.0%
! ATGCTACAAGCTCTTTTGGA+TGG + chr5.2:60110417-60110436 MS.gene53978:CDS 40.0%
! ATTTTCAGCTGCAGATGATG+TGG + chr5.2:60110020-60110039 MS.gene53978:CDS 40.0%
! ATTTTCCCCTGGATGAACAA+AGG - chr5.2:60110670-60110689 None:intergenic 40.0%
! CCTTATGCCTTTTCTAACTG+GGG + chr5.2:60111698-60111717 MS.gene53978:CDS 40.0%
! CGAAAACCTATTTAGTTCCC+CGG - chr5.2:60111330-60111349 None:intergenic 40.0%
! CGGGGAACTAAATAGGTTTT+CGG + chr5.2:60111328-60111347 MS.gene53978:CDS 40.0%
! GCCTTATGCCTTTTCTAACT+GGG + chr5.2:60111697-60111716 MS.gene53978:CDS 40.0%
! GGAACTAAATAGGTTTTCGG+GGG + chr5.2:60111331-60111350 MS.gene53978:CDS 40.0%
! GGGAACTAAATAGGTTTTCG+GGG + chr5.2:60111330-60111349 MS.gene53978:CDS 40.0%
! GGGGAACTAAATAGGTTTTC+GGG + chr5.2:60111329-60111348 MS.gene53978:CDS 40.0%
! TTTTCCCCTGGATGAACAAA+GGG - chr5.2:60110669-60110688 None:intergenic 40.0%
!! AAAAGATGGACTGTTAGGCA+TGG - chr5.2:60111282-60111301 None:intergenic 40.0%
!! AGCCTTATGCCTTTTCTAAC+TGG + chr5.2:60111696-60111715 MS.gene53978:CDS 40.0%
!! CAAAGTGAAGCCTTGTTCAA+GGG + chr5.2:60110617-60110636 MS.gene53978:CDS 40.0%
!! TCAAAGTGAAGCCTTGTTCA+AGG + chr5.2:60110616-60110635 MS.gene53978:CDS 40.0%
AGGAGATGGAATAGCAGAAC+CGG - chr5.2:60110925-60110944 None:intergenic 45.0%
AGGCATGGAATGATTCCAGT+TGG - chr5.2:60111267-60111286 None:intergenic 45.0%
AGTGTCCCTTTGTTCATCCA+GGG + chr5.2:60110661-60110680 MS.gene53978:CDS 45.0%
ATACGACCGGGGAACTAAAT+AGG + chr5.2:60111321-60111340 MS.gene53978:CDS 45.0%
ATCCTGCGCAGTATAAGACA+CGG + chr5.2:60110814-60110833 MS.gene53978:CDS 45.0%
ATGAAGAGCAGTAGCACCAT+CGG - chr5.2:60110244-60110263 None:intergenic 45.0%
ATGATTCCAGTTGGAAGGAG+AGG - chr5.2:60111258-60111277 None:intergenic 45.0%
ATGGGGTGTGAGTATGTGAA+TGG + chr5.2:60109955-60109974 MS.gene53978:CDS 45.0%
CAACGGAACAGATTCGATTC+CGG + chr5.2:60111964-60111983 MS.gene53978:CDS 45.0%
CACCCGATGGAAATTTGGAT+TGG + chr5.2:60111723-60111742 MS.gene53978:CDS 45.0%
CCCAGATGGATCAAACCTAT+AGG - chr5.2:60111564-60111583 None:intergenic 45.0%
CGATCCAATCACACTAGAAG+TGG - chr5.2:60111537-60111556 None:intergenic 45.0%
CTCATGGTTGCAGCTATGTA+TGG + chr5.2:60110153-60110172 MS.gene53978:CDS 45.0%
CTCTTCATTGTGCGGTTGTT+GGG + chr5.2:60110256-60110275 MS.gene53978:CDS 45.0%
CTTTCCGAAACTCAGGACAA+GGG - chr5.2:60110734-60110753 None:intergenic 45.0%
GAGACCCGAAAATGTCTTCA+AGG - chr5.2:60111370-60111389 None:intergenic 45.0%
GAGGGTGGTATCAAAACAGA+TGG - chr5.2:60111014-60111033 None:intergenic 45.0%
GATGCTCCAGATGAAGTCAA+AGG - chr5.2:60111047-60111066 None:intergenic 45.0%
GATGGAGAACCAAGTGACAT+TGG - chr5.2:60110996-60111015 None:intergenic 45.0%
GGAGCAATTGTACATGGATC+AGG + chr5.2:60111994-60112013 MS.gene53978:CDS 45.0%
GTATAAGACACGGCTTTGCA+AGG + chr5.2:60110824-60110843 MS.gene53978:CDS 45.0%
GTCGAAGAAACCGGAAAAGA+TGG - chr5.2:60111296-60111315 None:intergenic 45.0%
TACAATTGCTCCAACCAAGC+CGG - chr5.2:60111986-60112005 None:intergenic 45.0%
TACATAGCTGCAACCATGAG+AGG - chr5.2:60110153-60110172 None:intergenic 45.0%
TCTGGAAGAGAGACATCAAC+AGG - chr5.2:60110549-60110568 None:intergenic 45.0%
TCTTCATTGTGCGGTTGTTG+GGG + chr5.2:60110257-60110276 MS.gene53978:CDS 45.0%
TGATTGGATCGCCAACCTAT+AGG + chr5.2:60111546-60111565 MS.gene53978:CDS 45.0%
TGCAGCTATGTATGGAAGCT+TGG + chr5.2:60110161-60110180 MS.gene53978:CDS 45.0%
TGCTACTGCTCTTCATTGTG+CGG + chr5.2:60110248-60110267 MS.gene53978:CDS 45.0%
TGCTTAGGGTTGAAACTCAG+CGG + chr5.2:60111192-60111211 MS.gene53978:CDS 45.0%
TGGATGAACAAAGGGACACT+CGG - chr5.2:60110661-60110680 None:intergenic 45.0%
TGGGGTGTGAGTATGTGAAT+GGG + chr5.2:60109956-60109975 MS.gene53978:CDS 45.0%
TTCATCCAGGGGAAAATGCA+AGG + chr5.2:60110673-60110692 MS.gene53978:CDS 45.0%
TTTAACTCCAGCTGCAGCTA+GGG + chr5.2:60111817-60111836 MS.gene53978:CDS 45.0%
! AACCGGTAGAAGCATACAAG+GGG - chr5.2:60110908-60110927 None:intergenic 45.0%
! AGAACCGGTAGAAGCATACA+AGG - chr5.2:60110910-60110929 None:intergenic 45.0%
! AGCTGGAGTTAAAGGAGTAC+TGG - chr5.2:60111810-60111829 None:intergenic 45.0%
! CAAGGGCATATTCTCATGAC+TGG + chr5.2:60110634-60110653 MS.gene53978:CDS 45.0%
! CCCCAGTTAGAAAAGGCATA+AGG - chr5.2:60111701-60111720 None:intergenic 45.0%
! CTACTGATGGATGAGATGTC+CGG + chr5.2:60111227-60111246 MS.gene53978:CDS 45.0%
! GAAAAGGCATAAGGCTTAGC+AGG - chr5.2:60111692-60111711 None:intergenic 45.0%
! GAACCGGTAGAAGCATACAA+GGG - chr5.2:60110909-60110928 None:intergenic 45.0%
! GAGGATGCTACAAGCTCTTT+TGG + chr5.2:60110413-60110432 MS.gene53978:CDS 45.0%
! GATTTTGCTTGATGCATCGG+CGG + chr5.2:60110308-60110327 MS.gene53978:CDS 45.0%
! GCCTAACAGTCCATCTTTTC+CGG + chr5.2:60111283-60111302 MS.gene53978:CDS 45.0%
! GCTCTTTTGGATGGTACAGA+CGG + chr5.2:60110426-60110445 MS.gene53978:CDS 45.0%
! GTATGGTTGGTCGAAGAAAC+CGG - chr5.2:60111305-60111324 None:intergenic 45.0%
! TATTTAGTTCCCCGGTCGTA+TGG - chr5.2:60111322-60111341 None:intergenic 45.0%
! TCCAGTCTTCAACCTGCTTT+TGG - chr5.2:60111138-60111157 None:intergenic 45.0%
!! ACCGGAAAAGATGGACTGTT+AGG - chr5.2:60111287-60111306 None:intergenic 45.0%
!! ATGTTGATGGGGTTGGTTTG+TGG + chr5.2:60110079-60110098 MS.gene53978:CDS 45.0%
!! CAGGTCGGGTTGATGTTAAT+CGG + chr5.2:60110208-60110227 MS.gene53978:CDS 45.0%
!! CCAGTCTTCAACCTGCTTTT+GGG - chr5.2:60111137-60111156 None:intergenic 45.0%
!! TGGAGTTAAAGGAGTACTGG+TGG - chr5.2:60111807-60111826 None:intergenic 45.0%
!! TTTTGGGAAGCTGAAGGGTA+GGG - chr5.2:60111121-60111140 None:intergenic 45.0%
!!! CACACGGTATTTTTGAGTGC+TGG + chr5.2:60110787-60110806 MS.gene53978:CDS 45.0%
AACGAGGGTTGCGATGTTGA+TGG + chr5.2:60110066-60110085 MS.gene53978:CDS 50.0%
ACACGGCTTTGCAAGGATGA+AGG + chr5.2:60110831-60110850 MS.gene53978:CDS 50.0%
ACGAGGGTTGCGATGTTGAT+GGG + chr5.2:60110067-60110086 MS.gene53978:CDS 50.0%
ATCAACTGGTCGGTTACCGT+TGG - chr5.2:60110352-60110371 None:intergenic 50.0%
ATTAGACGGTGTCTGCCACA+TGG - chr5.2:60111090-60111109 None:intergenic 50.0%
CACGATCCTTTCCGAAACTC+AGG - chr5.2:60110741-60110760 None:intergenic 50.0%
CCTTGTCCTGAGTTTCGGAA+AGG + chr5.2:60110732-60110751 MS.gene53978:CDS 50.0%
CCTTTCCGAAACTCAGGACA+AGG - chr5.2:60110735-60110754 None:intergenic 50.0%
CGGAAAGGATCGTGCAGTAA+AGG + chr5.2:60110747-60110766 MS.gene53978:CDS 50.0%
CTAGCTGCAGCTGGAGTTAA+AGG - chr5.2:60111818-60111837 None:intergenic 50.0%
CTGCAGCGCCATCTTAACAA+CGG + chr5.2:60111947-60111966 MS.gene53978:CDS 50.0%
CTTCATTGTGCGGTTGTTGG+GGG + chr5.2:60110258-60110277 MS.gene53978:CDS 50.0%
CTTCTTTGCTCACAAGGTCG+AGG + chr5.2:60110875-60110894 MS.gene53978:CDS 50.0%
CTTTAACTCCAGCTGCAGCT+AGG + chr5.2:60111816-60111835 MS.gene53978:CDS 50.0%
GAACAGATTCGATTCCGGCT+TGG + chr5.2:60111969-60111988 MS.gene53978:CDS 50.0%
GAGTGTCCCTTTGTTCATCC+AGG + chr5.2:60110660-60110679 MS.gene53978:CDS 50.0%
GCGAAAGCAGCAACTACTGA+TGG + chr5.2:60111214-60111233 MS.gene53978:CDS 50.0%
GCTCCAGATGAAGTCAAAGG+CGG - chr5.2:60111044-60111063 None:intergenic 50.0%
GCTCTTCATTGTGCGGTTGT+TGG + chr5.2:60110255-60110274 MS.gene53978:CDS 50.0%
GCTTGTAGCATCCTCTTCCT+TGG - chr5.2:60110408-60110427 None:intergenic 50.0%
GGACGCATTTGGATTCCAGT+TGG - chr5.2:60111470-60111489 None:intergenic 50.0%
GTCTTTCCTCTCCTTCCAAC+TGG + chr5.2:60111249-60111268 MS.gene53978:CDS 50.0%
GTGTCCCTTTGTTCATCCAG+GGG + chr5.2:60110662-60110681 MS.gene53978:CDS 50.0%
GTTGGAAGGAGAGGAAAGAC+CGG - chr5.2:60111249-60111268 None:intergenic 50.0%
TCAGCTGCAGATGATGTGGT+TGG + chr5.2:60110024-60110043 MS.gene53978:CDS 50.0%
TCTTCGACCAACCATACGAC+CGG + chr5.2:60111308-60111327 MS.gene53978:CDS 50.0%
TCTTGCATTCAAAGCCGACG+CGG - chr5.2:60111594-60111613 None:intergenic 50.0%
TGAAGAGAGGACACCTCTCA+TGG + chr5.2:60110137-60110156 MS.gene53978:CDS 50.0%
! AATCGGTCTAGTGGATCCGA+TGG + chr5.2:60110225-60110244 MS.gene53978:CDS 50.0%
! CCCAAAAGCAGGTTGAAGAC+TGG + chr5.2:60111134-60111153 MS.gene53978:CDS 50.0%
! GAGCTGCTGCCTTTTCAAAG+CGG + chr5.2:60111612-60111631 MS.gene53978:CDS 50.0%
! GCTAGTGCTGTTGATGCCAA+CGG + chr5.2:60110333-60110352 MS.gene53978:CDS 50.0%
!! ACCTGCTTTTGGGAAGCTGA+AGG - chr5.2:60111127-60111146 None:intergenic 50.0%
!! AGATTCGATTCCGGCTTGGT+TGG + chr5.2:60111973-60111992 MS.gene53978:CDS 50.0%
!! CCTGCTTTTGGGAAGCTGAA+GGG - chr5.2:60111126-60111145 None:intergenic 50.0%
!! CTTTTGGGAAGCTGAAGGGT+AGG - chr5.2:60111122-60111141 None:intergenic 50.0%
!! GATGGGGTTGGTTTGTGGTA+TGG + chr5.2:60110084-60110103 MS.gene53978:CDS 50.0%
!! GGGTTGGTTTGTGGTATGGA+AGG + chr5.2:60110088-60110107 MS.gene53978:CDS 50.0%
AGCCGTGTCTTATACTGCGC+AGG - chr5.2:60110819-60110838 None:intergenic 55.0%
AGGGGAAAATGCAAGGCGAC+GGG + chr5.2:60110680-60110699 MS.gene53978:CDS 55.0%
ATCTGGGGAGTTATCCGCGT+CGG + chr5.2:60111577-60111596 MS.gene53978:CDS 55.0%
CACTGCGCTCGATAAAGCTC+TGG - chr5.2:60111640-60111659 None:intergenic 55.0%
CAGGGGAAAATGCAAGGCGA+CGG + chr5.2:60110679-60110698 MS.gene53978:CDS 55.0%
CCCTTCAGCTTCCCAAAAGC+AGG + chr5.2:60111123-60111142 MS.gene53978:CDS 55.0%
CGAGGGTTGCGATGTTGATG+GGG + chr5.2:60110068-60110087 MS.gene53978:CDS 55.0%
CGCCCCTTGTATGCTTCTAC+CGG + chr5.2:60110903-60110922 MS.gene53978:CDS 55.0%
CTTCGACCAACCATACGACC+GGG + chr5.2:60111309-60111328 MS.gene53978:CDS 55.0%
GCGTCCCTTGTCCTGAGTTT+CGG + chr5.2:60110727-60110746 MS.gene53978:CDS 55.0%
GGGCTCACCCGATGGAAATT+TGG + chr5.2:60111718-60111737 MS.gene53978:CDS 55.0%
GGGTGAGCCCCAGTTAGAAA+AGG - chr5.2:60111708-60111727 None:intergenic 55.0%
TAGTTCCCCGGTCGTATGGT+TGG - chr5.2:60111318-60111337 None:intergenic 55.0%
TGAAGTCAAAGGCGGCGTTG+AGG - chr5.2:60111036-60111055 None:intergenic 55.0%
TTCGACCAACCATACGACCG+GGG + chr5.2:60111310-60111329 MS.gene53978:CDS 55.0%
! ACGCCGCCTTTGACTTCATC+TGG + chr5.2:60111038-60111057 MS.gene53978:CDS 55.0%
! GGTTGCGATGTTGATGGGGT+TGG + chr5.2:60110072-60110091 MS.gene53978:CDS 55.0%
!! GCTCTGGCTCCGCTTTGAAA+AGG - chr5.2:60111624-60111643 None:intergenic 55.0%
CACATGGCTGCAGCAGCAAC+GGG - chr5.2:60111074-60111093 None:intergenic 60.0%
CCACATGGCTGCAGCAGCAA+CGG - chr5.2:60111075-60111094 None:intergenic 60.0%
CTTGATGCTGCAGGAGCAGC+AGG + chr5.2:60111428-60111447 MS.gene53978:CDS 60.0%
GACGCGGATAACTCCCCAGA+TGG - chr5.2:60111578-60111597 None:intergenic 60.0%
TCTAACTGGGGCTCACCCGA+TGG + chr5.2:60111710-60111729 MS.gene53978:CDS 60.0%
! AGTCAAAGGCGGCGTTGAGG+AGG - chr5.2:60111033-60111052 None:intergenic 60.0%
! CGTCGCCTTGCATTTTCCCC+TGG - chr5.2:60110681-60110700 None:intergenic 60.0%
! GTCAAAGGCGGCGTTGAGGA+GGG - chr5.2:60111032-60111051 None:intergenic 60.0%
CCGTTGCTGCTGCAGCCATG+TGG + chr5.2:60111072-60111091 MS.gene53978:CDS 65.0%
TCCAGCTGCAGCTAGGGCAC+AGG + chr5.2:60111823-60111842 MS.gene53978:CDS 65.0%
TCCTGTGCCCTAGCTGCAGC+TGG - chr5.2:60111827-60111846 None:intergenic 65.0%
! AAAGGCGGCGTTGAGGAGGG+TGG - chr5.2:60111029-60111048 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 60109910 60112033 60109910 ID=MS.gene53978
chr5.2 mRNA 60109910 60112033 60109910 ID=MS.gene53978.t1;Parent=MS.gene53978
chr5.2 exon 60109910 60112033 60109910 ID=MS.gene53978.t1.exon1;Parent=MS.gene53978.t1
chr5.2 CDS 60109910 60112033 60109910 ID=cds.MS.gene53978.t1;Parent=MS.gene53978.t1
Gene Sequence

>MS.gene53978

ATGTGCAGTGATTCTAAGAAAAAAACTTGTCAAAAGGATTTGATCATGGGGTGTGAGTATGTGAATGGGAATAAAGAAGGGCAGTATCATGAGGTTTCTTATTTGCTTGAATTTTCAGCTGCAGATGATGTGGTTGGTTTAAAAAATGCGATTGAAAACGAGGGTTGCGATGTTGATGGGGTTGGTTTGTGGTATGGAAGGAAAGTTGGTTCAAACAAATTAGGTTATGAAGAGAGGACACCTCTCATGGTTGCAGCTATGTATGGAAGCTTGGATGTATCGGCTTATATTCTTGGAACAGGTCGGGTTGATGTTAATCGGTCTAGTGGATCCGATGGTGCTACTGCTCTTCATTGTGCGGTTGTTGGGGGTTCTGCTGCTTCCCCCGAGATTATCAAGATTTTGCTTGATGCATCGGCGGATGCTAGTGCTGTTGATGCCAACGGTAACCGACCAGTTGATTTGATTGTTTCGCTGGCCAATTCTATCTTCAATCCAAGGAAGAGGATGCTACAAGCTCTTTTGGATGGTACAGACGGTGCTGATCAGACATGTCTTCTGTTTCCGGAGACAATTGATGACATTGACGAATATCAAAGGCAAGATGTAAACACGCCTCGTGTTTCAAAAGATTATCCTGTTGATGTCTCTCTTCCAGACATTAAGAATGGGATATATAGTACCGATGAGTTTAGGATGTATACGTTCAAAGTGAAGCCTTGTTCAAGGGCATATTCTCATGACTGGACCGAGTGTCCCTTTGTTCATCCAGGGGAAAATGCAAGGCGACGGGATCCAAGAAAATATCATTATAGCTGCGTCCCTTGTCCTGAGTTTCGGAAAGGATCGTGCAGTAAAGGAGATTCTTGTGACTATGCACACGGTATTTTTGAGTGCTGGCTTCATCCTGCGCAGTATAAGACACGGCTTTGCAAGGATGAAGGTTCATGCATGAGAAGAGTTTGCTTCTTTGCTCACAAGGTCGAGGAGCTTCGCCCCTTGTATGCTTCTACCGGTTCTGCTATTCCATCTCCTAGATCATATTACAGTACTGCTTCTACATTAGAGATGGGTTCAATTAGCCCAATGTCACTTGGTTCTCCATCTGTTTTGATACCACCCTCCTCAACGCCGCCTTTGACTTCATCTGGAGCATCGTCTCCCGTTGCTGCTGCAGCCATGTGGCAGACACCGTCTAATGTTTCGATCCCTACCCTTCAGCTTCCCAAAAGCAGGTTGAAGACTGGAATGACCGCTAGAGATATTAATTCAGATATTGCAATGCTTAGGGTTGAAACTCAGCGGCGAAAGCAGCAACTACTGATGGATGAGATGTCCGGTCTTTCCTCTCCTTCCAACTGGAATCATTCCATGCCTAACAGTCCATCTTTTCCGGTTTCTTCGACCAACCATACGACCGGGGAACTAAATAGGTTTTCGGGGGTGAATCCTACAAACCTTGAAGACATTTTCGGGTCTCTTGATCCATCAATGTTGCATAAATTCCACAGAATTTCACTTGATGCTGCAGGAGCAGCAGGATCTCAGTTACAATCTCCAACTGGAATCCAAATGCGTCCGAATATGAATCAGCATCTGCAACAAAACTATTCTTCAGGCCATTCCACTTCTAGTGTGATTGGATCGCCAACCTATAGGTTTGATCCATCTGGGGAGTTATCCGCGTCGGCTTTGAATGCAAGAGCTGCTGCCTTTTCAAAGCGGAGCCAGAGCTTTATCGAGCGCAGTGTCACAAACCGTCATTCTGAGCTTCATTCTCCTGCTAAGCCTTATGCCTTTTCTAACTGGGGCTCACCCGATGGAAATTTGGATTGGACTTCACACGGAGAAGAACTGAATAAGCTGAGGAAATCATCTTCATTTGGATTTCGAACCACCAGTACTCCTTTAACTCCAGCTGCAGCTAGGGCACAGGAAAATGACTATGAACCAGATGTGTCTTGGGTTAATTCGCTTGTAAAGGATGCTACGCCGCAAGAATCTCATCAGTTTAGCGTTGAAGATCAGAAAAGGAAACTGCAGCGCCATCTTAACAACGGAACAGATTCGATTCCGGCTTGGTTGGAGCAATTGTACATGGATCAGGAGCAAATAGTGCAGTGA
Protein sequence

>MS.gene53978.t1

MCSDSKKKTCQKDLIMGCEYVNGNKEGQYHEVSYLLEFSAADDVVGLKNAIENEGCDVDGVGLWYGRKVGSNKLGYEERTPLMVAAMYGSLDVSAYILGTGRVDVNRSSGSDGATALHCAVVGGSAASPEIIKILLDASADASAVDANGNRPVDLIVSLANSIFNPRKRMLQALLDGTDGADQTCLLFPETIDDIDEYQRQDVNTPRVSKDYPVDVSLPDIKNGIYSTDEFRMYTFKVKPCSRAYSHDWTECPFVHPGENARRRDPRKYHYSCVPCPEFRKGSCSKGDSCDYAHGIFECWLHPAQYKTRLCKDEGSCMRRVCFFAHKVEELRPLYASTGSAIPSPRSYYSTASTLEMGSISPMSLGSPSVLIPPSSTPPLTSSGASSPVAAAAMWQTPSNVSIPTLQLPKSRLKTGMTARDINSDIAMLRVETQRRKQQLLMDEMSGLSSPSNWNHSMPNSPSFPVSSTNHTTGELNRFSGVNPTNLEDIFGSLDPSMLHKFHRISLDAAGAAGSQLQSPTGIQMRPNMNQHLQQNYSSGHSTSSVIGSPTYRFDPSGELSASALNARAAAFSKRSQSFIERSVTNRHSELHSPAKPYAFSNWGSPDGNLDWTSHGEELNKLRKSSSFGFRTTSTPLTPAAARAQENDYEPDVSWVNSLVKDATPQESHQFSVEDQKRKLQRHLNNGTDSIPAWLEQLYMDQEQIVQ