AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94236

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94236.t1 MTR_5g082830 97.754 668 15 0 1 668 16 683 0.0 1358
MS.gene94236.t1 MTR_5g071070 49.206 693 298 13 10 667 30 703 0.0 632
MS.gene94236.t1 MTR_5g071070 49.219 640 271 13 63 667 1 621 0.0 576
MS.gene94236.t1 MTR_1g075890 45.345 666 271 16 10 628 28 647 6.32e-173 508
MS.gene94236.t1 MTR_7g092070 42.483 725 306 21 15 668 47 731 1.17e-166 495
MS.gene94236.t1 MTR_3g070880 62.000 400 87 7 1 399 1 336 7.47e-165 483
MS.gene94236.t1 MTR_3g070880 51.452 241 89 9 424 659 298 515 1.31e-53 192
MS.gene94236.t1 MTR_3g029590 39.807 726 322 20 13 663 43 728 6.38e-146 442
MS.gene94236.t1 MTR_3g464260 46.485 441 159 10 18 448 28 401 1.84e-116 359
MS.gene94236.t1 MTR_3g464260 31.050 219 105 9 462 668 345 529 1.57e-13 73.9
MS.gene94236.t1 MTR_3g464280 40.065 614 220 19 2 609 19 490 1.06e-110 343
MS.gene94236.t1 MTR_1g050438 37.262 526 255 13 15 494 15 511 1.60e-103 327
MS.gene94236.t1 MTR_0728s0020 37.262 526 255 13 15 494 31 527 6.10e-103 327
MS.gene94236.t1 MTR_3g435590 44.416 394 183 7 13 381 30 412 4.93e-102 320
MS.gene94236.t1 MTR_3g435680 46.048 291 119 6 118 383 44 321 3.00e-75 247
MS.gene94236.t1 MTR_3g464290 36.008 511 209 17 135 625 39 451 3.82e-71 238
MS.gene94236.t1 MTR_3g464270 35.831 427 192 15 201 623 8 356 1.27e-57 199
MS.gene94236.t1 MTR_8g102100 49.296 213 99 4 198 405 77 285 2.00e-55 194
MS.gene94236.t1 MTR_6g090380 68.519 108 34 0 197 304 98 205 1.45e-51 183
MS.gene94236.t1 MTR_3g103960 64.035 114 40 1 192 304 43 156 2.62e-48 171
MS.gene94236.t1 MTR_2g090605 64.865 111 37 1 202 310 77 187 6.74e-48 174
MS.gene94236.t1 MTR_4g078660 60.748 107 42 0 198 304 56 162 6.43e-46 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94236.t1 AT2G40140 51.108 677 222 19 1 668 20 596 0.0 592
MS.gene94236.t1 AT2G40140 51.108 677 222 19 1 668 20 596 0.0 592
MS.gene94236.t1 AT5G58620 49.478 671 249 15 13 668 12 607 0.0 587
MS.gene94236.t1 AT3G55980 61.954 389 130 6 14 398 29 403 1.51e-162 479
MS.gene94236.t1 AT3G55980 46.277 188 65 7 483 668 427 580 2.18e-28 120
MS.gene94236.t1 AT3G55980 61.954 389 130 6 14 398 96 470 1.26e-161 479
MS.gene94236.t1 AT3G55980 46.277 188 65 7 483 668 494 647 3.91e-28 120
MS.gene94236.t1 AT5G12850 40.470 724 307 25 10 663 37 706 9.57e-143 433
MS.gene94236.t1 AT2G41900 40.225 711 323 19 15 668 48 713 4.12e-142 431
MS.gene94236.t1 AT5G07500 62.698 126 47 0 179 304 29 154 2.50e-55 189
MS.gene94236.t1 AT4G29190 45.575 226 113 3 180 405 58 273 5.80e-54 189
MS.gene94236.t1 AT2G25900 68.224 107 34 0 198 304 86 192 2.74e-51 181
MS.gene94236.t1 AT2G25900 68.224 107 34 0 198 304 86 192 2.74e-51 181
MS.gene94236.t1 AT2G19810 63.559 118 43 0 187 304 63 180 7.91e-51 181
MS.gene94236.t1 AT1G03790 62.097 124 43 2 198 317 78 201 5.58e-48 174
MS.gene94236.t1 AT5G44260 62.963 108 39 1 198 304 56 163 2.91e-44 163

Find 200 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGAAGGTGGTTCATGTTT+TGG 0.196801 5.4:-65527366 None:intergenic
CTGAGGAAATCCGCTTCTTT+TGG 0.197101 5.4:+65527670 MS.gene94236:CDS
CTGCCACATGTTTCCACTCT+TGG 0.215837 5.4:-65526978 None:intergenic
CTTATGATTGCTTCCTTGTT+TGG 0.250098 5.4:+65526086 MS.gene94236:CDS
GACATAAACAACGGTGTATA+TGG 0.256569 5.4:+65526476 MS.gene94236:CDS
ACTCGCAAGAATCTCCTTTC+TGG 0.258493 5.4:-65526670 None:intergenic
CGATTTGGACAACGAATTAA+AGG 0.268967 5.4:-65526318 None:intergenic
TGTAGTTGAGATAGTAAATT+AGG 0.275386 5.4:-65527259 None:intergenic
ATCTGTTGTGTGGGTGTTGA+TGG 0.295601 5.4:-65527298 None:intergenic
GTCGTCCGGCCTTTCAGATT+GGG 0.298046 5.4:+65527594 MS.gene94236:CDS
TACACAGGGCGCAGCTCTTC+GGG 0.300339 5.4:-65526800 None:intergenic
TGTCGTCCGGCCTTTCAGAT+TGG 0.305721 5.4:+65527593 MS.gene94236:CDS
GCTCTTCATTGTGCTGTTGC+TGG 0.308167 5.4:+65526188 MS.gene94236:CDS
CAACACCTTCCTACGCGATT+TGG 0.321673 5.4:-65526333 None:intergenic
TTGGGTTAATTCATTGGTTA+AGG 0.328824 5.4:+65527753 MS.gene94236:CDS
TCTGAGAATTCCGGCGTATA+TGG 0.334049 5.4:+65527784 MS.gene94236:CDS
TGCCACATGTTTCCACTCTT+GGG 0.340074 5.4:-65526977 None:intergenic
CAGCTGCAGTGATGAATTCT+AGG 0.343853 5.4:+65527470 MS.gene94236:CDS
AGAAGTAGATGAGAAGGGTT+TGG 0.348805 5.4:+65525989 MS.gene94236:CDS
TTTGGCCCATGTTCATGTTT+TGG 0.358068 5.4:-65527348 None:intergenic
CGCCTTCCAACCCTTCCTGC+AGG 0.359580 5.4:+65527569 MS.gene94236:CDS
AGGAGTTTGTGGTTCCGATA+TGG 0.367167 5.4:+65526160 MS.gene94236:CDS
TGTCTCTTGGGTTAATTCAT+TGG 0.367834 5.4:+65527747 MS.gene94236:CDS
ATACACAGGGCGCAGCTCTT+CGG 0.370872 5.4:-65526801 None:intergenic
TATATGGATCAGATGAGTTT+AGG 0.370932 5.4:+65526492 MS.gene94236:CDS
ATTTGAATGAAGAAGGCTTT+TGG 0.383843 5.4:+65526012 MS.gene94236:CDS
CTCCTCCTTGCATTCTCACC+TGG 0.394967 5.4:-65526587 None:intergenic
CCCCACTGGGAAACTCGATT+GGG 0.408358 5.4:+65527621 MS.gene94236:CDS
CAAGACCCTTTGCGAAACTC+TGG 0.410056 5.4:-65526647 None:intergenic
ATGGAGGATAAGCTTCAAAA+AGG 0.412138 5.4:+65525903 MS.gene94236:CDS
GTCAATTCCACTAGCTGAAT+AGG 0.414997 5.4:-65526858 None:intergenic
GAGGAAGGCATATTGTTTGA+TGG 0.415999 5.4:-65527397 None:intergenic
CGCAAAGGGTCTTGCCAGAA+AGG 0.418356 5.4:+65526656 MS.gene94236:CDS
TGGGATGCAGTTGGTGCTGC+TGG 0.418675 5.4:-65527709 None:intergenic
GTCAAGCTCTTGCTTGATGC+CGG 0.418825 5.4:+65526236 MS.gene94236:CDS
TATGGTGAGGGCTTCCTCAC+TGG 0.419808 5.4:-65527421 None:intergenic
CGTCCTTGCAAAGCCTTGTT+CGG 0.424614 5.4:-65526736 None:intergenic
CAGCTGTAAGGGTATTTCCT+TGG 0.426047 5.4:-65526614 None:intergenic
ATTGATGTCAAAGGAGTTTG+TGG 0.426631 5.4:+65526149 MS.gene94236:CDS
TCGGGCAACGATATATCAAT+AGG 0.428410 5.4:-65526455 None:intergenic
GTCGAGTATATTCTTTCGAC+AGG 0.429694 5.4:+65526122 MS.gene94236:CDS
CTGCAGCTGCCGCATATTCT+CGG 0.430426 5.4:-65527809 None:intergenic
AAGAGGTCATCAAGGTTAGT+TGG 0.431942 5.4:-65527223 None:intergenic
GTGAGGAAGCCCTCACCATA+TGG 0.432783 5.4:+65527424 MS.gene94236:CDS
GCACTCTTCAAACGGCTACC+TGG 0.432870 5.4:-65527037 None:intergenic
CGCCCTGTGTATGCCTCCAC+TGG 0.433195 5.4:+65526812 MS.gene94236:CDS
GATGTTCCATCTGAGAATTC+CGG 0.433513 5.4:+65527775 MS.gene94236:CDS
AGACATCGGGCTCGGTATGT+TGG 0.435368 5.4:-65527729 None:intergenic
AGCTGTAAGGGTATTTCCTT+GGG 0.442755 5.4:-65526613 None:intergenic
CCACTAGCTGAATAGGACTT+TGG 0.443786 5.4:-65526851 None:intergenic
GACATCGGGCTCGGTATGTT+GGG 0.444418 5.4:-65527728 None:intergenic
TTCTGTTGAATTCGCTGTTA+CGG 0.446795 5.4:-65527186 None:intergenic
GGGCAGGGAACACAGCTGTA+AGG 0.457289 5.4:-65526626 None:intergenic
GTTTAGGATGTACAGTTTCA+AGG 0.458638 5.4:+65526508 MS.gene94236:CDS
TATACACCGTTGTTTATGTC+GGG 0.462321 5.4:-65526473 None:intergenic
GGTTTGGATTTGAATGAAGA+AGG 0.464213 5.4:+65526005 MS.gene94236:CDS
TACCGAGCCCGATGTCTCTT+GGG 0.465711 5.4:+65527735 MS.gene94236:CDS
CCCTTTGCGAAACTCTGGGC+AGG 0.465980 5.4:-65526642 None:intergenic
CACTCAAAGCACTCTTCAAA+CGG 0.468740 5.4:-65527045 None:intergenic
TGAGATGGAATTGCTCGGTC+TGG 0.469920 5.4:+65527084 MS.gene94236:CDS
ATACCGAGCCCGATGTCTCT+TGG 0.470119 5.4:+65527734 MS.gene94236:CDS
GGCTTCCTCACTGGAGAGGA+AGG 0.472160 5.4:-65527412 None:intergenic
CTACCTGGGAGCTGCAACGA+TGG 0.472506 5.4:-65527022 None:intergenic
CAAGAGAGAAGTAGATGAGA+AGG 0.474046 5.4:+65525983 MS.gene94236:CDS
TGGCGCATCTGCATTGCACT+TGG 0.475619 5.4:-65527328 None:intergenic
TTCTCACCTGGATGAACAAA+GGG 0.476487 5.4:-65526575 None:intergenic
AAGAGAGAAGTAGATGAGAA+GGG 0.478152 5.4:+65525984 MS.gene94236:CDS
AACAAGGAAGCAATCATAAG+AGG 0.482396 5.4:-65526083 None:intergenic
GCTGGCGCAATCAGATCAAC+CGG 0.486158 5.4:-65526296 None:intergenic
CACTCTTCAAACGGCTACCT+GGG 0.486258 5.4:-65527036 None:intergenic
CACCACGGTCAATGAAACTT+TGG 0.491260 5.4:-65527519 None:intergenic
GGTGCTGCAACTCACCATCT+TGG 0.497686 5.4:+65527538 MS.gene94236:CDS
CCTTCCTGCAGGGTGTCGTC+CGG 0.501860 5.4:+65527580 MS.gene94236:CDS
AACACCACCCATGTCTCCCT+TGG 0.506352 5.4:+65526940 MS.gene94236:CDS
AAACTCGATTGGGGTGTAAA+CGG 0.506355 5.4:+65527631 MS.gene94236:CDS
ATGAAGAGCAGTGGCCATAT+CGG 0.506470 5.4:-65526174 None:intergenic
TGTGGGTGTTGATGGTTGCA+TGG 0.506859 5.4:-65527290 None:intergenic
AGGCTTTGCAAGGACGAGAC+TGG 0.506964 5.4:+65526743 MS.gene94236:CDS
AACCCAAGAGACATCGGGCT+CGG 0.507762 5.4:-65527737 None:intergenic
CACTCTAGTGCTTCCAAACA+AGG 0.508719 5.4:-65526099 None:intergenic
TGAAGAGTGCTTTGAGTGCA+AGG 0.510749 5.4:+65527050 MS.gene94236:CDS
TTGGCTGCGCTGCTTTGCAA+AGG 0.511458 5.4:-65527500 None:intergenic
AATTCGCTGTTACGGAAGGA+TGG 0.512383 5.4:-65527178 None:intergenic
CTTCATTGTGCTGTTGCTGG+TGG 0.512690 5.4:+65526191 MS.gene94236:CDS
TCGTCCGGCCTTTCAGATTG+GGG 0.513214 5.4:+65527595 MS.gene94236:CDS
AGCCTTGTTCGGTATTGTGC+GGG 0.515045 5.4:-65526725 None:intergenic
GCCTTCCAACCCTTCCTGCA+GGG 0.517193 5.4:+65527570 MS.gene94236:CDS
CCACCCATGTCTCCCTTGGC+AGG 0.517296 5.4:+65526944 MS.gene94236:CDS
TTGTACATCTGCTCCACCCA+TGG 0.518688 5.4:-65527862 None:intergenic
TGATGCTAGTGGAAACAAAC+CGG 0.520501 5.4:+65526277 MS.gene94236:CDS
AATTTACTATCTCAACTACA+TGG 0.520900 5.4:+65527262 MS.gene94236:CDS
TCGAGACGTGCTTCCACCAT+GGG 0.522786 5.4:+65527846 MS.gene94236:CDS
TTCATCCAGGTGAGAATGCA+AGG 0.523324 5.4:+65526582 MS.gene94236:CDS
GGAACCCCAATCTGAAAGGC+CGG 0.526956 5.4:-65527599 None:intergenic
AAGAATTGGATCGAAGAAGA+TGG 0.527469 5.4:+65526043 MS.gene94236:CDS
CGACACCCTGCAGGAAGGGT+TGG 0.530365 5.4:-65527575 None:intergenic
TCGGTATGTTGGGATGCAGT+TGG 0.533279 5.4:-65527718 None:intergenic
GTACAAAGAGCAGGAGCAGA+TGG 0.534247 5.4:+65527879 MS.gene94236:CDS
CCACCATCGTTGCAGCTCCC+AGG 0.534409 5.4:+65527019 MS.gene94236:CDS
CCCTGCCCAGAGTTTCGCAA+AGG 0.536018 5.4:+65526641 MS.gene94236:CDS
GGAGCAGATGTACAAAGAGC+AGG 0.537466 5.4:+65527870 MS.gene94236:CDS
CGGACGACACCCTGCAGGAA+GGG 0.538863 5.4:-65527579 None:intergenic
CCAAAGTCCTATTCAGCTAG+TGG 0.541639 5.4:+65526851 MS.gene94236:CDS
ATTCGCTGTTACGGAAGGAT+GGG 0.542511 5.4:-65527177 None:intergenic
CCTGCCCAGAGTTTCGCAAA+GGG 0.543026 5.4:+65526642 MS.gene94236:CDS
GAGTGTCCCTTTGTTCATCC+AGG 0.543113 5.4:+65526569 MS.gene94236:CDS
TGAATTAACCCAAGAGACAT+CGG 0.547817 5.4:-65527743 None:intergenic
ATTTCTGAACCCAAAAGAAG+CGG 0.548536 5.4:-65527680 None:intergenic
GTTGTCCAAATCGCGTAGGA+AGG 0.549554 5.4:+65526328 MS.gene94236:CDS
GAATTAACCCAAGAGACATC+GGG 0.551012 5.4:-65527742 None:intergenic
GGGAGATGATGAACCTGCCA+AGG 0.553827 5.4:-65526957 None:intergenic
AGAAGATGGAATCTGAAAAG+AGG 0.553960 5.4:+65526057 MS.gene94236:CDS
CGAGGGCTTACTCTCACGAT+TGG 0.553989 5.4:+65526543 MS.gene94236:CDS
ACCCTGCAGGAAGGGTTGGA+AGG 0.554901 5.4:-65527571 None:intergenic
TGGAGAATGCATCTGTTGTG+TGG 0.555021 5.4:-65527308 None:intergenic
GTTGAATTCGCTGTTACGGA+AGG 0.555631 5.4:-65527182 None:intergenic
TTGGACAACGAATTAAAGGC+TGG 0.557548 5.4:-65526314 None:intergenic
GGCAGGGAACACAGCTGTAA+GGG 0.559340 5.4:-65526625 None:intergenic
ATATACACCGTTGTTTATGT+CGG 0.561652 5.4:-65526474 None:intergenic
GCTGATGAATCAAATCCATA+TGG 0.562833 5.4:-65527439 None:intergenic
AAGCCTTGTTCGGTATTGTG+CGG 0.563056 5.4:-65526726 None:intergenic
AAGACCCTTTGCGAAACTCT+GGG 0.564593 5.4:-65526646 None:intergenic
GAACCAGTGGAGGCATACAC+AGG 0.565086 5.4:-65526815 None:intergenic
CATCACTGCAGCTGCTACTG+CGG 0.565349 5.4:-65527461 None:intergenic
AAGGCCGGACGACACCCTGC+AGG 0.568796 5.4:-65527584 None:intergenic
GATCTTGAGATGGAATTGCT+CGG 0.572830 5.4:+65527079 MS.gene94236:CDS
CCTGGGAGCTGCAACGATGG+TGG 0.572925 5.4:-65527019 None:intergenic
GATGCGCCAAAACATGAACA+TGG 0.573046 5.4:+65527342 MS.gene94236:CDS
AGACGTGCTTCCACCATGGG+TGG 0.573955 5.4:+65527849 MS.gene94236:CDS
CTTGTTTGGAAGCACTAGAG+TGG 0.576155 5.4:+65526100 MS.gene94236:CDS
TGAACCTGCCAAGGGAGACA+TGG 0.576779 5.4:-65526948 None:intergenic
AGACAATTCAAGCAAGATTG+AGG 0.577524 5.4:-65525937 None:intergenic
ATCCCGCACAATACCGAACA+AGG 0.579739 5.4:+65526723 MS.gene94236:CDS
AAGACAATCAGCATCTGCAC+CGG 0.580324 5.4:-65526255 None:intergenic
GTCGAGACGTGCTTCCACCA+TGG 0.580527 5.4:+65527845 MS.gene94236:CDS
TGAGAGTAAGCCCTCGAGCA+TGG 0.580982 5.4:-65526536 None:intergenic
ATGGATAACTTGCGCGAAGG+TGG 0.584205 5.4:-65527378 None:intergenic
AAGGAGCGTCCATCTCCCCT+TGG 0.584326 5.4:-65526394 None:intergenic
TTCGCTGTTACGGAAGGATG+GGG 0.584831 5.4:-65527176 None:intergenic
TGAACTCATCCAAGGGGAGA+TGG 0.584869 5.4:+65526385 MS.gene94236:CDS
CAAATCGCGTAGGAAGGTGT+TGG 0.585250 5.4:+65526334 MS.gene94236:CDS
GCTGATTGTCTTGATGCTAG+TGG 0.585414 5.4:+65526266 MS.gene94236:CDS
CCGGACGACACCCTGCAGGA+AGG 0.586791 5.4:-65527580 None:intergenic
GGAGAATGCATCTGTTGTGT+GGG 0.590736 5.4:-65527307 None:intergenic
GAAGAGTGCTTTGAGTGCAA+GGG 0.591839 5.4:+65527051 MS.gene94236:CDS
ATTTACTATCTCAACTACAT+GGG 0.597158 5.4:+65527263 MS.gene94236:CDS
ATACCGAACAAGGCTTTGCA+AGG 0.600596 5.4:+65526733 MS.gene94236:CDS
TAGGATCAGCTGATGCTAAG+AGG 0.602013 5.4:-65527240 None:intergenic
TGCTGCTGCTGTTGACGACG+AGG 0.602651 5.4:-65527112 None:intergenic
CAAGGTGAAGCCATGCTCGA+GGG 0.603335 5.4:+65526526 MS.gene94236:CDS
TCAAGGTGAAGCCATGCTCG+AGG 0.606783 5.4:+65526525 MS.gene94236:CDS
GAATCAAATCCATATGGTGA+GGG 0.607337 5.4:-65527433 None:intergenic
GTAAGTGCTGAACTCATCCA+AGG 0.609847 5.4:+65526377 MS.gene94236:CDS
ATTCTCACCTGGATGAACAA+AGG 0.616516 5.4:-65526576 None:intergenic
TGGATGAACAAAGGGACACT+CGG 0.617153 5.4:-65526567 None:intergenic
GGTTCATCATCTCCCAAGAG+TGG 0.617657 5.4:+65526965 MS.gene94236:CDS
ACAGGGAAGATTGATGTCAA+AGG 0.618036 5.4:+65526140 MS.gene94236:CDS
CTGCAGGAAGGGTTGGAAGG+CGG 0.621056 5.4:-65527568 None:intergenic
GGTGTTGAAACAGTTGACAT+AGG 0.621294 5.4:-65526923 None:intergenic
ATTCGTTGTCCAAATCGCGT+AGG 0.621353 5.4:+65526324 MS.gene94236:CDS
CTGATGCTAAGAGGTCATCA+AGG 0.625709 5.4:-65527231 None:intergenic
TGAATCAAATCCATATGGTG+AGG 0.627563 5.4:-65527434 None:intergenic
GCTGAATAGGACTTTGGTGA+AGG 0.628923 5.4:-65526845 None:intergenic
AGGCATAGCTGAACCAGTGG+AGG 0.631941 5.4:-65526825 None:intergenic
CCCACTGGGAAACTCGATTG+GGG 0.632471 5.4:+65527622 MS.gene94236:CDS
TACATCTGCTCCACCCATGG+TGG 0.632978 5.4:-65527859 None:intergenic
ATGCAAGGAGGAGAGACCCA+AGG 0.635278 5.4:+65526597 MS.gene94236:CDS
GGAGATGATGAACCTGCCAA+GGG 0.637245 5.4:-65526956 None:intergenic
CCTTTGCGAAACTCTGGGCA+GGG 0.637831 5.4:-65526641 None:intergenic
GAACCTGCCAAGGGAGACAT+GGG 0.638207 5.4:-65526947 None:intergenic
AGCCAAAGTTTCATTGACCG+TGG 0.638346 5.4:+65527517 MS.gene94236:CDS
TGAGGGCTTCCTCACTGGAG+AGG 0.638650 5.4:-65527416 None:intergenic
AACAGCACAATGAAGAGCAG+TGG 0.643629 5.4:-65526183 None:intergenic
TATACGCCGGAATTCTCAGA+TGG 0.645414 5.4:-65527781 None:intergenic
TAAGTGCTGAACTCATCCAA+GGG 0.645605 5.4:+65526378 MS.gene94236:CDS
AACCAGTGGAGGCATACACA+GGG 0.645864 5.4:-65526814 None:intergenic
CCCCAATCGAGTTTCCCAGT+GGG 0.652420 5.4:-65527622 None:intergenic
GCTGCAACGATGGTGGAGTG+AGG 0.652628 5.4:-65527012 None:intergenic
CCCAATCGAGTTTCCCAGTG+GGG 0.653880 5.4:-65527621 None:intergenic
TATATGGTGCCGAGAATATG+CGG 0.657924 5.4:+65527800 MS.gene94236:CDS
ATGCGCCAAAACATGAACAT+GGG 0.661793 5.4:+65527343 MS.gene94236:CDS
CCAATCGAGTTTCCCAGTGG+GGG 0.662085 5.4:-65527620 None:intergenic
CTCCCAAGAGTGGAAACATG+TGG 0.663242 5.4:+65526975 MS.gene94236:CDS
AAGGGATCTCGATCTTGAGA+TGG 0.663328 5.4:+65527069 MS.gene94236:CDS
TGAAGGCATAGCTGAACCAG+TGG 0.664738 5.4:-65526828 None:intergenic
GCTGTTACGGAAGGATGGGG+AGG 0.665128 5.4:-65527173 None:intergenic
ACCCCAATCGAGTTTCCCAG+TGG 0.665168 5.4:-65527623 None:intergenic
AAGGCGGAGAAAGTCCAAGA+TGG 0.665195 5.4:-65527552 None:intergenic
GATTCTTGCGAGTATGCACA+TGG 0.667439 5.4:+65526680 MS.gene94236:CDS
GTGACGAGCTGAACAAGCTG+AGG 0.679618 5.4:+65527653 MS.gene94236:CDS
GATGGTGAGTTGCAGCACCA+CGG 0.682458 5.4:-65527534 None:intergenic
AGTTGACATAGGCAAAGATG+AGG 0.688585 5.4:-65526912 None:intergenic
TCGAGTATATTCTTTCGACA+GGG 0.693087 5.4:+65526123 MS.gene94236:CDS
ATTCTCGGCACCATATACGC+CGG 0.695958 5.4:-65527794 None:intergenic
TTGATGGATAACTTGCGCGA+AGG 0.700054 5.4:-65527381 None:intergenic
AAGTGCTGAACTCATCCAAG+GGG 0.716976 5.4:+65526379 MS.gene94236:CDS
ATATGCCTTCCTCTCCAGTG+AGG 0.717918 5.4:+65527407 MS.gene94236:CDS
TCGTTGCCCGACATAAACAA+CGG 0.735153 5.4:+65526467 MS.gene94236:CDS
CCTGCCAAGGGAGACATGGG+TGG 0.744203 5.4:-65526944 None:intergenic
ATCCAGGTGAGAATGCAAGG+AGG 0.764804 5.4:+65526585 MS.gene94236:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTTACTATCTCAACTACA+TGG + chr5.4:65527262-65527281 MS.gene94236:CDS 25.0%
! ATTTACTATCTCAACTACAT+GGG + chr5.4:65527263-65527282 MS.gene94236:CDS 25.0%
! TGTAGTTGAGATAGTAAATT+AGG - chr5.4:65527262-65527281 None:intergenic 25.0%
ATATACACCGTTGTTTATGT+CGG - chr5.4:65526477-65526496 None:intergenic 30.0%
CCTTTCTCATTGAAAAAAGA+AGG + chr5.4:65526413-65526432 MS.gene94236:CDS 30.0%
TATATGGATCAGATGAGTTT+AGG + chr5.4:65526492-65526511 MS.gene94236:CDS 30.0%
TTCTCATTGAAAAAAGAAGG+TGG + chr5.4:65526416-65526435 MS.gene94236:CDS 30.0%
! ATTTGAATGAAGAAGGCTTT+TGG + chr5.4:65526012-65526031 MS.gene94236:CDS 30.0%
! TTGGGTTAATTCATTGGTTA+AGG + chr5.4:65527753-65527772 MS.gene94236:CDS 30.0%
! TTTTGGTATGGTAGAAGAAT+TGG + chr5.4:65526029-65526048 MS.gene94236:CDS 30.0%
!!! CCTTCTTTTTTCAATGAGAA+AGG - chr5.4:65526416-65526435 None:intergenic 30.0%
!!! TCTTTTGGGTTCAGAAATAA+TGG + chr5.4:65527685-65527704 MS.gene94236:CDS 30.0%
AACAAGGAAGCAATCATAAG+AGG - chr5.4:65526086-65526105 None:intergenic 35.0%
AAGAATTGGATCGAAGAAGA+TGG + chr5.4:65526043-65526062 MS.gene94236:CDS 35.0%
AAGAGAGAAGTAGATGAGAA+GGG + chr5.4:65525984-65526003 MS.gene94236:CDS 35.0%
AGAAGATGGAATCTGAAAAG+AGG + chr5.4:65526057-65526076 MS.gene94236:CDS 35.0%
AGACAATTCAAGCAAGATTG+AGG - chr5.4:65525940-65525959 None:intergenic 35.0%
ATGGAGGATAAGCTTCAAAA+AGG + chr5.4:65525903-65525922 MS.gene94236:CDS 35.0%
ATTGATGTCAAAGGAGTTTG+TGG + chr5.4:65526149-65526168 MS.gene94236:CDS 35.0%
ATTTCTGAACCCAAAAGAAG+CGG - chr5.4:65527683-65527702 None:intergenic 35.0%
CTTATGATTGCTTCCTTGTT+TGG + chr5.4:65526086-65526105 MS.gene94236:CDS 35.0%
GAATCAAATCCATATGGTGA+GGG - chr5.4:65527436-65527455 None:intergenic 35.0%
GACATAAACAACGGTGTATA+TGG + chr5.4:65526476-65526495 MS.gene94236:CDS 35.0%
GCTGATGAATCAAATCCATA+TGG - chr5.4:65527442-65527461 None:intergenic 35.0%
GGTTTGGATTTGAATGAAGA+AGG + chr5.4:65526005-65526024 MS.gene94236:CDS 35.0%
TATACACCGTTGTTTATGTC+GGG - chr5.4:65526476-65526495 None:intergenic 35.0%
TCGAGTATATTCTTTCGACA+GGG + chr5.4:65526123-65526142 MS.gene94236:CDS 35.0%
TGAATCAAATCCATATGGTG+AGG - chr5.4:65527437-65527456 None:intergenic 35.0%
TGAATTAACCCAAGAGACAT+CGG - chr5.4:65527746-65527765 None:intergenic 35.0%
TGTCTCTTGGGTTAATTCAT+TGG + chr5.4:65527747-65527766 MS.gene94236:CDS 35.0%
TTCTGTTGAATTCGCTGTTA+CGG - chr5.4:65527189-65527208 None:intergenic 35.0%
! AATGAAGAAGGCTTTTGGTA+TGG + chr5.4:65526017-65526036 MS.gene94236:CDS 35.0%
! CGATTTGGACAACGAATTAA+AGG - chr5.4:65526321-65526340 None:intergenic 35.0%
! GTTTAGGATGTACAGTTTCA+AGG + chr5.4:65526508-65526527 MS.gene94236:CDS 35.0%
AAACTCGATTGGGGTGTAAA+CGG + chr5.4:65527631-65527650 MS.gene94236:CDS 40.0%
AAGAGGTCATCAAGGTTAGT+TGG - chr5.4:65527226-65527245 None:intergenic 40.0%
ACAGGGAAGATTGATGTCAA+AGG + chr5.4:65526140-65526159 MS.gene94236:CDS 40.0%
AGAAGTAGATGAGAAGGGTT+TGG + chr5.4:65525989-65526008 MS.gene94236:CDS 40.0%
AGTTGACATAGGCAAAGATG+AGG - chr5.4:65526915-65526934 None:intergenic 40.0%
ATGCGCCAAAACATGAACAT+GGG + chr5.4:65527343-65527362 MS.gene94236:CDS 40.0%
ATTCTCACCTGGATGAACAA+AGG - chr5.4:65526579-65526598 None:intergenic 40.0%
CAAGAGAGAAGTAGATGAGA+AGG + chr5.4:65525983-65526002 MS.gene94236:CDS 40.0%
GAATTAACCCAAGAGACATC+GGG - chr5.4:65527745-65527764 None:intergenic 40.0%
GATCTTGAGATGGAATTGCT+CGG + chr5.4:65527079-65527098 MS.gene94236:CDS 40.0%
GATGTTCCATCTGAGAATTC+CGG + chr5.4:65527775-65527794 MS.gene94236:CDS 40.0%
GTCAATTCCACTAGCTGAAT+AGG - chr5.4:65526861-65526880 None:intergenic 40.0%
GTCGAGTATATTCTTTCGAC+AGG + chr5.4:65526122-65526141 MS.gene94236:CDS 40.0%
TCGGGCAACGATATATCAAT+AGG - chr5.4:65526458-65526477 None:intergenic 40.0%
TGATGCTAGTGGAAACAAAC+CGG + chr5.4:65526277-65526296 MS.gene94236:CDS 40.0%
TTCTCACCTGGATGAACAAA+GGG - chr5.4:65526578-65526597 None:intergenic 40.0%
TTGGACAACGAATTAAAGGC+TGG - chr5.4:65526317-65526336 None:intergenic 40.0%
! CACTCAAAGCACTCTTCAAA+CGG - chr5.4:65527048-65527067 None:intergenic 40.0%
! GAGGAAGGCATATTGTTTGA+TGG - chr5.4:65527400-65527419 None:intergenic 40.0%
! GGTGTTGAAACAGTTGACAT+AGG - chr5.4:65526926-65526945 None:intergenic 40.0%
! TATATGGTGCCGAGAATATG+CGG + chr5.4:65527800-65527819 MS.gene94236:CDS 40.0%
! TGAGGAAATCCGCTTCTTTT+GGG + chr5.4:65527671-65527690 MS.gene94236:CDS 40.0%
! TTTGGCCCATGTTCATGTTT+TGG - chr5.4:65527351-65527370 None:intergenic 40.0%
!! AGCTGTAAGGGTATTTCCTT+GGG - chr5.4:65526616-65526635 None:intergenic 40.0%
!! GTGTTGGAGATGTTTTTGAG+AGG + chr5.4:65526350-65526369 MS.gene94236:CDS 40.0%
!! TAAGTGCTGAACTCATCCAA+GGG + chr5.4:65526378-65526397 MS.gene94236:CDS 40.0%
AACAGCACAATGAAGAGCAG+TGG - chr5.4:65526186-65526205 None:intergenic 45.0%
AAGACAATCAGCATCTGCAC+CGG - chr5.4:65526258-65526277 None:intergenic 45.0%
AAGACCCTTTGCGAAACTCT+GGG - chr5.4:65526649-65526668 None:intergenic 45.0%
AAGGGATCTCGATCTTGAGA+TGG + chr5.4:65527069-65527088 MS.gene94236:CDS 45.0%
AATTCGCTGTTACGGAAGGA+TGG - chr5.4:65527181-65527200 None:intergenic 45.0%
ACTCGCAAGAATCTCCTTTC+TGG - chr5.4:65526673-65526692 None:intergenic 45.0%
AGCCAAAGTTTCATTGACCG+TGG + chr5.4:65527517-65527536 MS.gene94236:CDS 45.0%
AGGAGTTTGTGGTTCCGATA+TGG + chr5.4:65526160-65526179 MS.gene94236:CDS 45.0%
ATACCGAACAAGGCTTTGCA+AGG + chr5.4:65526733-65526752 MS.gene94236:CDS 45.0%
ATGAAGAGCAGTGGCCATAT+CGG - chr5.4:65526177-65526196 None:intergenic 45.0%
ATTCGCTGTTACGGAAGGAT+GGG - chr5.4:65527180-65527199 None:intergenic 45.0%
ATTCGTTGTCCAAATCGCGT+AGG + chr5.4:65526324-65526343 MS.gene94236:CDS 45.0%
CACCACGGTCAATGAAACTT+TGG - chr5.4:65527522-65527541 None:intergenic 45.0%
CACTCTAGTGCTTCCAAACA+AGG - chr5.4:65526102-65526121 None:intergenic 45.0%
CAGCTGCAGTGATGAATTCT+AGG + chr5.4:65527470-65527489 MS.gene94236:CDS 45.0%
CCAAAGTCCTATTCAGCTAG+TGG + chr5.4:65526851-65526870 MS.gene94236:CDS 45.0%
CCACTAGCTGAATAGGACTT+TGG - chr5.4:65526854-65526873 None:intergenic 45.0%
CTGATGCTAAGAGGTCATCA+AGG - chr5.4:65527234-65527253 None:intergenic 45.0%
GATGCGCCAAAACATGAACA+TGG + chr5.4:65527342-65527361 MS.gene94236:CDS 45.0%
GATTCTTGCGAGTATGCACA+TGG + chr5.4:65526680-65526699 MS.gene94236:CDS 45.0%
GCTGAATAGGACTTTGGTGA+AGG - chr5.4:65526848-65526867 None:intergenic 45.0%
GCTGATTGTCTTGATGCTAG+TGG + chr5.4:65526266-65526285 MS.gene94236:CDS 45.0%
GGAGAATGCATCTGTTGTGT+GGG - chr5.4:65527310-65527329 None:intergenic 45.0%
GTTGAATTCGCTGTTACGGA+AGG - chr5.4:65527185-65527204 None:intergenic 45.0%
TAGGATCAGCTGATGCTAAG+AGG - chr5.4:65527243-65527262 None:intergenic 45.0%
TATACGCCGGAATTCTCAGA+TGG - chr5.4:65527784-65527803 None:intergenic 45.0%
TCGTTGCCCGACATAAACAA+CGG + chr5.4:65526467-65526486 MS.gene94236:CDS 45.0%
TCTGAGAATTCCGGCGTATA+TGG + chr5.4:65527784-65527803 MS.gene94236:CDS 45.0%
TGCCACATGTTTCCACTCTT+GGG - chr5.4:65526980-65526999 None:intergenic 45.0%
TGGAGAATGCATCTGTTGTG+TGG - chr5.4:65527311-65527330 None:intergenic 45.0%
TGGATGAACAAAGGGACACT+CGG - chr5.4:65526570-65526589 None:intergenic 45.0%
TTCATCCAGGTGAGAATGCA+AGG + chr5.4:65526582-65526601 MS.gene94236:CDS 45.0%
! CTGAGGAAATCCGCTTCTTT+TGG + chr5.4:65527670-65527689 MS.gene94236:CDS 45.0%
! TTGATGGATAACTTGCGCGA+AGG - chr5.4:65527384-65527403 None:intergenic 45.0%
!! AAGCCTTGTTCGGTATTGTG+CGG - chr5.4:65526729-65526748 None:intergenic 45.0%
!! AAGTGCTGAACTCATCCAAG+GGG + chr5.4:65526379-65526398 MS.gene94236:CDS 45.0%
!! ATCTGTTGTGTGGGTGTTGA+TGG - chr5.4:65527301-65527320 None:intergenic 45.0%
!! CAGCTGTAAGGGTATTTCCT+TGG - chr5.4:65526617-65526636 None:intergenic 45.0%
!! CTTGTTTGGAAGCACTAGAG+TGG + chr5.4:65526100-65526119 MS.gene94236:CDS 45.0%
!! GAAGAGTGCTTTGAGTGCAA+GGG + chr5.4:65527051-65527070 MS.gene94236:CDS 45.0%
!! GGAGATGTTTTTGAGAGGTG+AGG + chr5.4:65526355-65526374 MS.gene94236:CDS 45.0%
!! GTAAGTGCTGAACTCATCCA+AGG + chr5.4:65526377-65526396 MS.gene94236:CDS 45.0%
!! TGAAGAGTGCTTTGAGTGCA+AGG + chr5.4:65527050-65527069 MS.gene94236:CDS 45.0%
!!! CACATGGTGTTTTCGAGTCA+TGG + chr5.4:65526696-65526715 MS.gene94236:CDS 45.0%
AAGGCGGAGAAAGTCCAAGA+TGG - chr5.4:65527555-65527574 None:intergenic 50.0%
ATATGCCTTCCTCTCCAGTG+AGG + chr5.4:65527407-65527426 MS.gene94236:CDS 50.0%
ATCCAGGTGAGAATGCAAGG+AGG + chr5.4:65526585-65526604 MS.gene94236:CDS 50.0%
ATCCCGCACAATACCGAACA+AGG + chr5.4:65526723-65526742 MS.gene94236:CDS 50.0%
ATTCTCGGCACCATATACGC+CGG - chr5.4:65527797-65527816 None:intergenic 50.0%
CAACACCTTCCTACGCGATT+TGG - chr5.4:65526336-65526355 None:intergenic 50.0%
CAAGACCCTTTGCGAAACTC+TGG - chr5.4:65526650-65526669 None:intergenic 50.0%
CACTCTTCAAACGGCTACCT+GGG - chr5.4:65527039-65527058 None:intergenic 50.0%
CTCCCAAGAGTGGAAACATG+TGG + chr5.4:65526975-65526994 MS.gene94236:CDS 50.0%
CTGCCACATGTTTCCACTCT+TGG - chr5.4:65526981-65527000 None:intergenic 50.0%
GAGTGTCCCTTTGTTCATCC+AGG + chr5.4:65526569-65526588 MS.gene94236:CDS 50.0%
GGAGATGATGAACCTGCCAA+GGG - chr5.4:65526959-65526978 None:intergenic 50.0%
GGAGCAGATGTACAAAGAGC+AGG + chr5.4:65527870-65527889 MS.gene94236:CDS 50.0%
GGTTCATCATCTCCCAAGAG+TGG + chr5.4:65526965-65526984 MS.gene94236:CDS 50.0%
GTACAAAGAGCAGGAGCAGA+TGG + chr5.4:65527879-65527898 MS.gene94236:CDS 50.0%
GTCAAGCTCTTGCTTGATGC+CGG + chr5.4:65526236-65526255 MS.gene94236:CDS 50.0%
GTTGTCCAAATCGCGTAGGA+AGG + chr5.4:65526328-65526347 MS.gene94236:CDS 50.0%
TCGGTATGTTGGGATGCAGT+TGG - chr5.4:65527721-65527740 None:intergenic 50.0%
TGAACTCATCCAAGGGGAGA+TGG + chr5.4:65526385-65526404 MS.gene94236:CDS 50.0%
TGAGATGGAATTGCTCGGTC+TGG + chr5.4:65527084-65527103 MS.gene94236:CDS 50.0%
TTCGCTGTTACGGAAGGATG+GGG - chr5.4:65527179-65527198 None:intergenic 50.0%
TTGTACATCTGCTCCACCCA+TGG - chr5.4:65527865-65527884 None:intergenic 50.0%
! AACCAGTGGAGGCATACACA+GGG - chr5.4:65526817-65526836 None:intergenic 50.0%
! ATGGATAACTTGCGCGAAGG+TGG - chr5.4:65527381-65527400 None:intergenic 50.0%
! CAAATCGCGTAGGAAGGTGT+TGG + chr5.4:65526334-65526353 MS.gene94236:CDS 50.0%
! CGCGAAGGTGGTTCATGTTT+TGG - chr5.4:65527369-65527388 None:intergenic 50.0%
! CTTCATTGTGCTGTTGCTGG+TGG + chr5.4:65526191-65526210 MS.gene94236:CDS 50.0%
! GCTCTTCATTGTGCTGTTGC+TGG + chr5.4:65526188-65526207 MS.gene94236:CDS 50.0%
! TGAAGGCATAGCTGAACCAG+TGG - chr5.4:65526831-65526850 None:intergenic 50.0%
!! AGCCTTGTTCGGTATTGTGC+GGG - chr5.4:65526728-65526747 None:intergenic 50.0%
!! CGTCCTTGCAAAGCCTTGTT+CGG - chr5.4:65526739-65526758 None:intergenic 50.0%
!! TGTGGGTGTTGATGGTTGCA+TGG - chr5.4:65527293-65527312 None:intergenic 50.0%
!!! TGGTTCTGAGTTTTCGCTCG+AGG + chr5.4:65526211-65526230 MS.gene94236:CDS 50.0%
AACACCACCCATGTCTCCCT+TGG + chr5.4:65526940-65526959 MS.gene94236:CDS 55.0%
AACCCAAGAGACATCGGGCT+CGG - chr5.4:65527740-65527759 None:intergenic 55.0%
ACCCCAATCGAGTTTCCCAG+TGG - chr5.4:65527626-65527645 None:intergenic 55.0%
AGGCATAGCTGAACCAGTGG+AGG - chr5.4:65526828-65526847 None:intergenic 55.0%
AGGCTTTGCAAGGACGAGAC+TGG + chr5.4:65526743-65526762 MS.gene94236:CDS 55.0%
ATACACAGGGCGCAGCTCTT+CGG - chr5.4:65526804-65526823 None:intergenic 55.0%
ATACCGAGCCCGATGTCTCT+TGG + chr5.4:65527734-65527753 MS.gene94236:CDS 55.0%
ATGCAAGGAGGAGAGACCCA+AGG + chr5.4:65526597-65526616 MS.gene94236:CDS 55.0%
CAAGGTGAAGCCATGCTCGA+GGG + chr5.4:65526526-65526545 MS.gene94236:CDS 55.0%
CATCACTGCAGCTGCTACTG+CGG - chr5.4:65527464-65527483 None:intergenic 55.0%
CCAATCGAGTTTCCCAGTGG+GGG - chr5.4:65527623-65527642 None:intergenic 55.0%
CCCAATCGAGTTTCCCAGTG+GGG - chr5.4:65527624-65527643 None:intergenic 55.0%
CCCACTGGGAAACTCGATTG+GGG + chr5.4:65527622-65527641 MS.gene94236:CDS 55.0%
CCCCAATCGAGTTTCCCAGT+GGG - chr5.4:65527625-65527644 None:intergenic 55.0%
CCCCACTGGGAAACTCGATT+GGG + chr5.4:65527621-65527640 MS.gene94236:CDS 55.0%
CCTGCCCAGAGTTTCGCAAA+GGG + chr5.4:65526642-65526661 MS.gene94236:CDS 55.0%
CCTTTGCGAAACTCTGGGCA+GGG - chr5.4:65526644-65526663 None:intergenic 55.0%
CGAGGGCTTACTCTCACGAT+TGG + chr5.4:65526543-65526562 MS.gene94236:CDS 55.0%
CGCAAAGGGTCTTGCCAGAA+AGG + chr5.4:65526656-65526675 MS.gene94236:CDS 55.0%
CTCCTCCTTGCATTCTCACC+TGG - chr5.4:65526590-65526609 None:intergenic 55.0%
CTGCAGCTGCCGCATATTCT+CGG - chr5.4:65527812-65527831 None:intergenic 55.0%
GAACCTGCCAAGGGAGACAT+GGG - chr5.4:65526950-65526969 None:intergenic 55.0%
GATGGTGAGTTGCAGCACCA+CGG - chr5.4:65527537-65527556 None:intergenic 55.0%
GCACTCTTCAAACGGCTACC+TGG - chr5.4:65527040-65527059 None:intergenic 55.0%
GCTGGCGCAATCAGATCAAC+CGG - chr5.4:65526299-65526318 None:intergenic 55.0%
GGAACCCCAATCTGAAAGGC+CGG - chr5.4:65527602-65527621 None:intergenic 55.0%
GGCAGGGAACACAGCTGTAA+GGG - chr5.4:65526628-65526647 None:intergenic 55.0%
GGGAGATGATGAACCTGCCA+AGG - chr5.4:65526960-65526979 None:intergenic 55.0%
GTCGTCCGGCCTTTCAGATT+GGG + chr5.4:65527594-65527613 MS.gene94236:CDS 55.0%
GTGACGAGCTGAACAAGCTG+AGG + chr5.4:65527653-65527672 MS.gene94236:CDS 55.0%
GTGAGGAAGCCCTCACCATA+TGG + chr5.4:65527424-65527443 MS.gene94236:CDS 55.0%
TACATCTGCTCCACCCATGG+TGG - chr5.4:65527862-65527881 None:intergenic 55.0%
TACCGAGCCCGATGTCTCTT+GGG + chr5.4:65527735-65527754 MS.gene94236:CDS 55.0%
TATGGTGAGGGCTTCCTCAC+TGG - chr5.4:65527424-65527443 None:intergenic 55.0%
TCAAGGTGAAGCCATGCTCG+AGG + chr5.4:65526525-65526544 MS.gene94236:CDS 55.0%
TCGAGACGTGCTTCCACCAT+GGG + chr5.4:65527846-65527865 MS.gene94236:CDS 55.0%
TCGTCCGGCCTTTCAGATTG+GGG + chr5.4:65527595-65527614 MS.gene94236:CDS 55.0%
TGAACCTGCCAAGGGAGACA+TGG - chr5.4:65526951-65526970 None:intergenic 55.0%
TGAGAGTAAGCCCTCGAGCA+TGG - chr5.4:65526539-65526558 None:intergenic 55.0%
TGGCGCATCTGCATTGCACT+TGG - chr5.4:65527331-65527350 None:intergenic 55.0%
TGGGGGAACCCCAATCTGAA+AGG - chr5.4:65527606-65527625 None:intergenic 55.0%
TGTCGTCCGGCCTTTCAGAT+TGG + chr5.4:65527593-65527612 MS.gene94236:CDS 55.0%
! AGACATCGGGCTCGGTATGT+TGG - chr5.4:65527732-65527751 None:intergenic 55.0%
! GAACCAGTGGAGGCATACAC+AGG - chr5.4:65526818-65526837 None:intergenic 55.0%
! GACATCGGGCTCGGTATGTT+GGG - chr5.4:65527731-65527750 None:intergenic 55.0%
! GGTGCTGCAACTCACCATCT+TGG + chr5.4:65527538-65527557 MS.gene94236:CDS 55.0%
! TTGGCTGCGCTGCTTTGCAA+AGG - chr5.4:65527503-65527522 None:intergenic 55.0%
ACCCTGCAGGAAGGGTTGGA+AGG - chr5.4:65527574-65527593 None:intergenic 60.0%
AGACGTGCTTCCACCATGGG+TGG + chr5.4:65527849-65527868 MS.gene94236:CDS 60.0%
CAGATTGGGGTTCCCCCACT+GGG + chr5.4:65527608-65527627 MS.gene94236:CDS 60.0%
CCCCCACTGGGAAACTCGAT+TGG + chr5.4:65527620-65527639 MS.gene94236:CDS 60.0%
CCCTGCCCAGAGTTTCGCAA+AGG + chr5.4:65526641-65526660 MS.gene94236:CDS 60.0%
CCCTTTGCGAAACTCTGGGC+AGG - chr5.4:65526645-65526664 None:intergenic 60.0%
CTACCTGGGAGCTGCAACGA+TGG - chr5.4:65527025-65527044 None:intergenic 60.0%
CTGCAGGAAGGGTTGGAAGG+CGG - chr5.4:65527571-65527590 None:intergenic 60.0%
GCCTTCCAACCCTTCCTGCA+GGG + chr5.4:65527570-65527589 MS.gene94236:CDS 60.0%
GCTGTTACGGAAGGATGGGG+AGG - chr5.4:65527176-65527195 None:intergenic 60.0%
GGCTTCCTCACTGGAGAGGA+AGG - chr5.4:65527415-65527434 None:intergenic 60.0%
GGGCAGGGAACACAGCTGTA+AGG - chr5.4:65526629-65526648 None:intergenic 60.0%
GTCGAGACGTGCTTCCACCA+TGG + chr5.4:65527845-65527864 MS.gene94236:CDS 60.0%
TACACAGGGCGCAGCTCTTC+GGG - chr5.4:65526803-65526822 None:intergenic 60.0%
TCAGATTGGGGTTCCCCCAC+TGG + chr5.4:65527607-65527626 MS.gene94236:CDS 60.0%
TGAGGGCTTCCTCACTGGAG+AGG - chr5.4:65527419-65527438 None:intergenic 60.0%
TGCTGCTGCTGTTGACGACG+AGG - chr5.4:65527115-65527134 None:intergenic 60.0%
!! AAGGAGCGTCCATCTCCCCT+TGG - chr5.4:65526397-65526416 None:intergenic 60.0%
!! GCTGCAACGATGGTGGAGTG+AGG - chr5.4:65527015-65527034 None:intergenic 60.0%
!! TGGGATGCAGTTGGTGCTGC+TGG - chr5.4:65527712-65527731 None:intergenic 60.0%
CCACCATCGTTGCAGCTCCC+AGG + chr5.4:65527019-65527038 MS.gene94236:CDS 65.0%
CCACCCATGTCTCCCTTGGC+AGG + chr5.4:65526944-65526963 MS.gene94236:CDS 65.0%
CCTGCCAAGGGAGACATGGG+TGG - chr5.4:65526947-65526966 None:intergenic 65.0%
CCTGGGAGCTGCAACGATGG+TGG - chr5.4:65527022-65527041 None:intergenic 65.0%
CGACACCCTGCAGGAAGGGT+TGG - chr5.4:65527578-65527597 None:intergenic 65.0%
CGCCCTGTGTATGCCTCCAC+TGG + chr5.4:65526812-65526831 MS.gene94236:CDS 65.0%
CGCCTTCCAACCCTTCCTGC+AGG + chr5.4:65527569-65527588 MS.gene94236:CDS 65.0%
CGGACGACACCCTGCAGGAA+GGG - chr5.4:65527582-65527601 None:intergenic 65.0%
!! CCTTCCTGCAGGGTGTCGTC+CGG + chr5.4:65527580-65527599 MS.gene94236:CDS 65.0%
AAGGCCGGACGACACCCTGC+AGG - chr5.4:65527587-65527606 None:intergenic 70.0%
CCGGACGACACCCTGCAGGA+AGG - chr5.4:65527583-65527602 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 65525903 65527909 65525903 ID=MS.gene94236
chr5.4 mRNA 65525903 65527909 65525903 ID=MS.gene94236.t1;Parent=MS.gene94236
chr5.4 exon 65525903 65527909 65525903 ID=MS.gene94236.t1.exon1;Parent=MS.gene94236.t1
chr5.4 CDS 65525903 65527909 65525903 ID=cds.MS.gene94236.t1;Parent=MS.gene94236.t1
Gene Sequence

>MS.gene94236

ATGGAGGATAAGCTTCAAAAAGGTGTTCACAACTCCTCAATCTTGCTTGAATTGTCTGCATCTGATGATTTTGATGCTTTCAAGAGAGAAGTAGATGAGAAGGGTTTGGATTTGAATGAAGAAGGCTTTTGGTATGGTAGAAGAATTGGATCGAAGAAGATGGAATCTGAAAAGAGGACTCCTCTTATGATTGCTTCCTTGTTTGGAAGCACTAGAGTGGTCGAGTATATTCTTTCGACAGGGAAGATTGATGTCAAAGGAGTTTGTGGTTCCGATATGGCCACTGCTCTTCATTGTGCTGTTGCTGGTGGTTCTGAGTTTTCGCTCGAGGTTGTCAAGCTCTTGCTTGATGCCGGTGCAGATGCTGATTGTCTTGATGCTAGTGGAAACAAACCGGTTGATCTGATTGCGCCAGCCTTTAATTCGTTGTCCAAATCGCGTAGGAAGGTGTTGGAGATGTTTTTGAGAGGTGAGGTAAGTGCTGAACTCATCCAAGGGGAGATGGACGCTCCTTTCTCATTGAAAAAAGAAGGTGGTGATAAGAAAGAGTTTCCTATTGATATATCGTTGCCCGACATAAACAACGGTGTATATGGATCAGATGAGTTTAGGATGTACAGTTTCAAGGTGAAGCCATGCTCGAGGGCTTACTCTCACGATTGGACCGAGTGTCCCTTTGTTCATCCAGGTGAGAATGCAAGGAGGAGAGACCCAAGGAAATACCCTTACAGCTGTGTTCCCTGCCCAGAGTTTCGCAAAGGGTCTTGCCAGAAAGGAGATTCTTGCGAGTATGCACATGGTGTTTTCGAGTCATGGCTACATCCCGCACAATACCGAACAAGGCTTTGCAAGGACGAGACTGGCTGCAATAGAAAAGTCTGCTTCTTTGCTCACAGACCCGAAGAGCTGCGCCCTGTGTATGCCTCCACTGGTTCAGCTATGCCTTCACCAAAGTCCTATTCAGCTAGTGGAATTGACATGACATCGATGAGTTCGTTGTCTCTTAGCTCCTCATCTTTGCCTATGTCAACTGTTTCAACACCACCCATGTCTCCCTTGGCAGGTTCATCATCTCCCAAGAGTGGAAACATGTGGCAGAACAAATTAAACCTCACTCCACCATCGTTGCAGCTCCCAGGTAGCCGTTTGAAGAGTGCTTTGAGTGCAAGGGATCTCGATCTTGAGATGGAATTGCTCGGTCTGGATAGTCCTCGTCGTCAACAGCAGCAGCAACAACAACAACAATTGATTGAAGAGATTGCTAGAATCTCCTCCCCATCCTTCCGTAACAGCGAATTCAACAGAATTGCTGATTTGAATCCAACTAACCTTGATGACCTCTTAGCATCAGCTGATCCTAATTTACTATCTCAACTACATGGGCTTTCCATGCAACCATCAACACCCACACAACAGATGCATTCTCCAAGTGCAATGCAGATGCGCCAAAACATGAACATGGGCCAAAACATGAACCACCTTCGCGCAAGTTATCCATCAAACAATATGCCTTCCTCTCCAGTGAGGAAGCCCTCACCATATGGATTTGATTCATCAGCCGCAGTAGCAGCTGCAGTGATGAATTCTAGGTCTGCTGCCTTTGCAAAGCAGCGCAGCCAAAGTTTCATTGACCGTGGTGCTGCAACTCACCATCTTGGACTTTCTCCGCCTTCCAACCCTTCCTGCAGGGTGTCGTCCGGCCTTTCAGATTGGGGTTCCCCCACTGGGAAACTCGATTGGGGTGTAAACGGTGACGAGCTGAACAAGCTGAGGAAATCCGCTTCTTTTGGGTTCAGAAATAATGGACCAGCAGCACCAACTGCATCCCAACATACCGAGCCCGATGTCTCTTGGGTTAATTCATTGGTTAAGGATGTTCCATCTGAGAATTCCGGCGTATATGGTGCCGAGAATATGCGGCAGCTGCAGCAATATGATCTGAGTCGAGACGTGCTTCCACCATGGGTGGAGCAGATGTACAAAGAGCAGGAGCAGATGGTAGCATGA
Protein sequence

>MS.gene94236.t1

MEDKLQKGVHNSSILLELSASDDFDAFKREVDEKGLDLNEEGFWYGRRIGSKKMESEKRTPLMIASLFGSTRVVEYILSTGKIDVKGVCGSDMATALHCAVAGGSEFSLEVVKLLLDAGADADCLDASGNKPVDLIAPAFNSLSKSRRKVLEMFLRGEVSAELIQGEMDAPFSLKKEGGDKKEFPIDISLPDINNGVYGSDEFRMYSFKVKPCSRAYSHDWTECPFVHPGENARRRDPRKYPYSCVPCPEFRKGSCQKGDSCEYAHGVFESWLHPAQYRTRLCKDETGCNRKVCFFAHRPEELRPVYASTGSAMPSPKSYSASGIDMTSMSSLSLSSSSLPMSTVSTPPMSPLAGSSSPKSGNMWQNKLNLTPPSLQLPGSRLKSALSARDLDLEMELLGLDSPRRQQQQQQQQQLIEEIARISSPSFRNSEFNRIADLNPTNLDDLLASADPNLLSQLHGLSMQPSTPTQQMHSPSAMQMRQNMNMGQNMNHLRASYPSNNMPSSPVRKPSPYGFDSSAAVAAAVMNSRSAAFAKQRSQSFIDRGAATHHLGLSPPSNPSCRVSSGLSDWGSPTGKLDWGVNGDELNKLRKSASFGFRNNGPAAPTASQHTEPDVSWVNSLVKDVPSENSGVYGAENMRQLQQYDLSRDVLPPWVEQMYKEQEQMVA