AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47959


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47959.t1 MTR_8g089655 98.077 104 2 0 1 104 43 146 1.53e-71 209
MS.gene47959.t1 MTR_8g089655 98.077 104 2 0 1 104 40 143 1.89e-71 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47959.t1 AT1G16790 55.670 97 40 2 1 95 39 134 2.72e-33 113

Find 26 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCAATTGGGGTTGTTTC+TGG 0.158557 4.1:+11014255 MS.gene47959:CDS
GTTTCTGGTGCTGCTCTTAA+TGG 0.209111 4.1:+11014270 MS.gene47959:CDS
TTCATCTGGTAAAGGTAAAA+AGG 0.279247 4.1:+11014131 MS.gene47959:CDS
TTTCACCTATTGCTCAGATT+TGG 0.309135 4.1:+11014319 MS.gene47959:CDS
GATTTACAGAAGGCGTTGTT+TGG 0.358146 4.1:+11014102 MS.gene47959:CDS
GGTGCTGCTCTTAATGGAAT+TGG 0.362930 4.1:+11014276 MS.gene47959:CDS
TTGTTGAGACGTTTGCAATT+GGG 0.377010 4.1:+11014244 MS.gene47959:CDS
TTTGTTGAGACGTTTGCAAT+TGG 0.400517 4.1:+11014243 MS.gene47959:CDS
GAATTCCAAATCTGAGCAAT+AGG 0.408372 4.1:-11014324 None:intergenic
TTGTTTGGAGTAATTTCATC+TGG 0.412151 4.1:+11014117 MS.gene47959:CDS
AAATGAATCTTCTAGAAAGA+TGG 0.419369 4.1:+11014164 MS.gene47959:CDS
TCATTGAGGGGTAGATTTAG+TGG 0.436309 4.1:-11014358 None:intergenic
ATGAAGCTTGATACTAAGCC+TGG 0.457946 4.1:+11014219 MS.gene47959:CDS
GGAGTAATTTCATCTGGTAA+AGG 0.473380 4.1:+11014123 MS.gene47959:CDS
TGTTGAGACGTTTGCAATTG+GGG 0.507433 4.1:+11014245 MS.gene47959:CDS
ATCTTCTAGAAAGATGGATT+CGG 0.522036 4.1:+11014170 MS.gene47959:CDS
AGGGGTAGATTTAGTGGCTG+AGG 0.534389 4.1:-11014352 None:intergenic
AGGGTGCAAAATGTCATTGA+GGG 0.536281 4.1:-11014371 None:intergenic
TTCACAAGAAGCCTCATTGA+AGG 0.549868 4.1:+11014071 MS.gene47959:CDS
AAGGGTGCAAAATGTCATTG+AGG 0.553529 4.1:-11014372 None:intergenic
GCAAACGTCTCAACAAATCC+AGG 0.561055 4.1:-11014237 None:intergenic
AGCCTCATTGAAGGTCATAG+AGG 0.621483 4.1:+11014080 MS.gene47959:CDS
GGTCATAGAGGATTTACAGA+AGG 0.636264 4.1:+11014092 MS.gene47959:CDS
ATCCTCTATGACCTTCAATG+AGG 0.666837 4.1:-11014082 None:intergenic
GCAATAGGTGAAAGAATGTG+AGG 0.689368 4.1:-11014309 None:intergenic
GGGTGCAAAATGTCATTGAG+GGG 0.694593 4.1:-11014370 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAATCTTCTAGAAAGA+TGG + chr4.1:11014164-11014183 MS.gene47959:CDS 25.0%
TTCATCTGGTAAAGGTAAAA+AGG + chr4.1:11014131-11014150 MS.gene47959:CDS 30.0%
! ATCTTCTAGAAAGATGGATT+CGG + chr4.1:11014170-11014189 MS.gene47959:CDS 30.0%
! TTGTTTGGAGTAATTTCATC+TGG + chr4.1:11014117-11014136 MS.gene47959:CDS 30.0%
CTGGTAAAGGTAAAAAGGAT+AGG + chr4.1:11014136-11014155 MS.gene47959:CDS 35.0%
GAATTCCAAATCTGAGCAAT+AGG - chr4.1:11014327-11014346 None:intergenic 35.0%
GGAGTAATTTCATCTGGTAA+AGG + chr4.1:11014123-11014142 MS.gene47959:CDS 35.0%
TTGTTGAGACGTTTGCAATT+GGG + chr4.1:11014244-11014263 MS.gene47959:CDS 35.0%
TTTCACCTATTGCTCAGATT+TGG + chr4.1:11014319-11014338 MS.gene47959:CDS 35.0%
TTTGTTGAGACGTTTGCAAT+TGG + chr4.1:11014243-11014262 MS.gene47959:CDS 35.0%
ATCCTCTATGACCTTCAATG+AGG - chr4.1:11014085-11014104 None:intergenic 40.0%
ATGAAGCTTGATACTAAGCC+TGG + chr4.1:11014219-11014238 MS.gene47959:CDS 40.0%
GCAATAGGTGAAAGAATGTG+AGG - chr4.1:11014312-11014331 None:intergenic 40.0%
GGTCATAGAGGATTTACAGA+AGG + chr4.1:11014092-11014111 MS.gene47959:CDS 40.0%
TCATTGAGGGGTAGATTTAG+TGG - chr4.1:11014361-11014380 None:intergenic 40.0%
TGTTGAGACGTTTGCAATTG+GGG + chr4.1:11014245-11014264 MS.gene47959:CDS 40.0%
TTCACAAGAAGCCTCATTGA+AGG + chr4.1:11014071-11014090 MS.gene47959:CDS 40.0%
! TTTGCAATTGGGGTTGTTTC+TGG + chr4.1:11014255-11014274 MS.gene47959:CDS 40.0%
!! GATTTACAGAAGGCGTTGTT+TGG + chr4.1:11014102-11014121 MS.gene47959:CDS 40.0%
AGCCTCATTGAAGGTCATAG+AGG + chr4.1:11014080-11014099 MS.gene47959:CDS 45.0%
GCAAACGTCTCAACAAATCC+AGG - chr4.1:11014240-11014259 None:intergenic 45.0%
!! GGTGCTGCTCTTAATGGAAT+TGG + chr4.1:11014276-11014295 MS.gene47959:CDS 45.0%
!! GTTTCTGGTGCTGCTCTTAA+TGG + chr4.1:11014270-11014289 MS.gene47959:CDS 45.0%
AGGGGTAGATTTAGTGGCTG+AGG - chr4.1:11014355-11014374 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 11014066 11014380 11014066 ID=MS.gene47959
chr4.1 mRNA 11014066 11014380 11014066 ID=MS.gene47959.t1;Parent=MS.gene47959
chr4.1 exon 11014066 11014380 11014066 ID=MS.gene47959.t1.exon1;Parent=MS.gene47959.t1
chr4.1 CDS 11014066 11014380 11014066 ID=cds.MS.gene47959.t1;Parent=MS.gene47959.t1
Gene Sequence

>MS.gene47959

ATGGATTCACAAGAAGCCTCATTGAAGGTCATAGAGGATTTACAGAAGGCGTTGTTTGGAGTAATTTCATCTGGTAAAGGTAAAAAGGATAGGCTTATAAATGAATCTTCTAGAAAGATGGATTCGGTTAATCAAAGACTTGCTATACTTGATATGAAGCTTGATACTAAGCCTGGATTTGTTGAGACGTTTGCAATTGGGGTTGTTTCTGGTGCTGCTCTTAATGGAATTGGTGCTGTAATGCCTCACATTCTTTCACCTATTGCTCAGATTTGGAATTCTGTTACCTCAGCCACTAAATCTACCCCTCAATGA

Protein sequence

>MS.gene47959.t1

MDSQEASLKVIEDLQKALFGVISSGKGKKDRLINESSRKMDSVNQRLAILDMKLDTKPGFVETFAIGVVSGAALNGIGAVMPHILSPIAQIWNSVTSATKSTPQ