Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49571.t1 | XP_003613597.1 | 91.2 | 159 | 12 | 1 | 1 | 157 | 1 | 159 | 3.70E-54 | 221.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49571.t1 | O23342 | 67.1 | 70 | 23 | 0 | 88 | 157 | 108 | 177 | 2.1e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49571.t1 | G7K9T4 | 91.2 | 159 | 12 | 1 | 1 | 157 | 1 | 159 | 2.6e-54 | 221.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene49571 | 0.810295 | 1.24E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene49571 | 0.828373 | 9.32E-55 | -1.69E-46 |
| MS.gene050558 | MS.gene49571 | 0.838619 | 2.58E-57 | -1.69E-46 |
| MS.gene051582 | MS.gene49571 | 0.808141 | 3.58E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene49571 | 0.858913 | 5.94E-63 | -1.69E-46 |
| MS.gene054473 | MS.gene49571 | 0.819236 | 1.29E-52 | -1.69E-46 |
| MS.gene054930 | MS.gene49571 | 0.814621 | 1.40E-51 | -1.69E-46 |
| MS.gene054983 | MS.gene49571 | 0.800276 | 1.55E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene49571 | 0.831034 | 2.10E-55 | -1.69E-46 |
| MS.gene055428 | MS.gene49571 | 0.830493 | 2.85E-55 | -1.69E-46 |
| MS.gene055712 | MS.gene49571 | 0.811512 | 6.75E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene49571 | 0.804643 | 1.96E-49 | -1.69E-46 |
| MS.gene057121 | MS.gene49571 | 0.805561 | 1.26E-49 | -1.69E-46 |
| MS.gene057439 | MS.gene49571 | 0.820103 | 8.19E-53 | -1.69E-46 |
| MS.gene058002 | MS.gene49571 | 0.833702 | 4.58E-56 | -1.69E-46 |
| MS.gene058142 | MS.gene49571 | 0.82765 | 1.39E-54 | -1.69E-46 |
| MS.gene05886 | MS.gene49571 | 0.806097 | 9.71E-50 | -1.69E-46 |
| MS.gene059597 | MS.gene49571 | 0.819335 | 1.23E-52 | -1.69E-46 |
| MS.gene059899 | MS.gene49571 | 0.80157 | 8.46E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene49571 | 0.817145 | 3.84E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49571.t1 | MTR_5g038520 | 89.937 | 159 | 14 | 1 | 1 | 157 | 1 | 159 | 4.10e-91 | 262 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49571.t1 | AT4G14870 | 42.778 | 180 | 67 | 8 | 11 | 157 | 1 | 177 | 7.51e-32 | 112 |
Find 42 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAATAGAATGGCCTGAATT+TGG | 0.155669 | 5.1:-29214350 | MS.gene49571:CDS |
| GTTGATTCTGGTTTCGGTTC+TGG | 0.225493 | 5.1:+29214470 | None:intergenic |
| TCTGGTTTCGGTTCTGGTTC+GGG | 0.248024 | 5.1:+29214476 | None:intergenic |
| AATTCGGACTCGGTTGATTC+TGG | 0.250082 | 5.1:+29214458 | None:intergenic |
| TTCTGGTTCGGGGTTGGAGG+TGG | 0.251891 | 5.1:+29214487 | None:intergenic |
| AGAAAGAAGGAGACAATTTC+TGG | 0.259067 | 5.1:-29214397 | MS.gene49571:CDS |
| GCCTGAATTTGGGAAAGTTT+TGG | 0.263374 | 5.1:-29214339 | MS.gene49571:CDS |
| AAGAGAAGGGAGTGAGGGTT+TGG | 0.280510 | 5.1:+29214562 | None:intergenic |
| CCCAAAACTTTCCCAAATTC+AGG | 0.294145 | 5.1:+29214338 | None:intergenic |
| AATTGAGGACAGGGAGTAGA+AGG | 0.296228 | 5.1:+29214599 | None:intergenic |
| TTCTGGTTTCGGTTCTGGTT+CGG | 0.300899 | 5.1:+29214475 | None:intergenic |
| GACTCGGTTGATTCTGGTTT+CGG | 0.302160 | 5.1:+29214464 | None:intergenic |
| CTTCAATTCCGATGCTAATT+CGG | 0.305857 | 5.1:+29214442 | None:intergenic |
| GTTGTTCTTAGTGTTATCTT+TGG | 0.334231 | 5.1:-29214305 | MS.gene49571:CDS |
| AAATAGAATGGCCTGAATTT+GGG | 0.352322 | 5.1:-29214349 | MS.gene49571:CDS |
| AGAGAAGGGAGTGAGGGTTT+GGG | 0.386040 | 5.1:+29214563 | None:intergenic |
| CGGTTCTGGTTCGGGGTTGG+AGG | 0.407735 | 5.1:+29214484 | None:intergenic |
| TTTCGGTTCTGGTTCGGGGT+TGG | 0.415510 | 5.1:+29214481 | None:intergenic |
| TCTGGTTCGGGGTTGGAGGT+GGG | 0.446629 | 5.1:+29214488 | None:intergenic |
| GAGAGTAAGAGAAGGGAGTG+AGG | 0.446761 | 5.1:+29214556 | None:intergenic |
| GTTCGGGGTTGGAGGTGGGT+TGG | 0.449439 | 5.1:+29214492 | None:intergenic |
| AACGGCGTGGTTTCAGAAAT+CGG | 0.462382 | 5.1:-29214374 | MS.gene49571:CDS |
| GAAGTGGAAGAATTGAGGAC+AGG | 0.468061 | 5.1:+29214589 | None:intergenic |
| GGTTTGAAGTGGAAGAATTG+AGG | 0.486748 | 5.1:+29214584 | None:intergenic |
| CAGAAATCGGTGAAATAGAA+TGG | 0.492882 | 5.1:-29214361 | MS.gene49571:CDS |
| AAGTGGAAGAATTGAGGACA+GGG | 0.494677 | 5.1:+29214590 | None:intergenic |
| CAAGAGAGGAAAGAGAAAGA+AGG | 0.500510 | 5.1:-29214410 | MS.gene49571:CDS |
| GTGAGGGTTTGGGTTTGAAG+TGG | 0.502213 | 5.1:+29214573 | None:intergenic |
| CTGGTTTCGGTTCTGGTTCG+GGG | 0.532553 | 5.1:+29214477 | None:intergenic |
| TTCCGATGCTAATTCGGACT+CGG | 0.534253 | 5.1:+29214448 | None:intergenic |
| AGAGTAAGAGAAGGGAGTGA+GGG | 0.535792 | 5.1:+29214557 | None:intergenic |
| TGAAGAAAGCAATGCAAGAG+AGG | 0.560185 | 5.1:-29214424 | MS.gene49571:CDS |
| CGGCGCGTGAGAGTAAGAGA+AGG | 0.562454 | 5.1:+29214548 | None:intergenic |
| TGAGGACAGGGAGTAGAAGG+TGG | 0.568073 | 5.1:+29214602 | None:intergenic |
| AGAGAGTGTGAGTGTAAAGC+TGG | 0.590584 | 5.1:+29214643 | None:intergenic |
| CGGCGCGTGGAAGTGTGAGT+CGG | 0.600147 | 5.1:+29214528 | None:intergenic |
| GAAGGAGACAATTTCTGGAA+CGG | 0.601112 | 5.1:-29214392 | MS.gene49571:CDS |
| CACACTTCCACGCGCCGTCG+AGG | 0.607708 | 5.1:-29214522 | MS.gene49571:CDS |
| GGCGCGTGAGAGTAAGAGAA+GGG | 0.614359 | 5.1:+29214549 | None:intergenic |
| AACCGAGTCCGAATTAGCAT+CGG | 0.616271 | 5.1:-29214450 | MS.gene49571:CDS |
| GGTGGTGATGATGTAAAGTG+TGG | 0.662522 | 5.1:+29214620 | None:intergenic |
| AGACAATTTCTGGAACGGCG+TGG | 0.696429 | 5.1:-29214387 | MS.gene49571:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAGAATGGCCTGAATTT+GGG | - | chr5.1:29214524-29214543 | MS.gene49571:CDS | 30.0% | |
| GTTGTTCTTAGTGTTATCTT+TGG | - | chr5.1:29214568-29214587 | MS.gene49571:CDS | 30.0% | |
| ! | ATAAAGTTTTTGCTGGCAAA+GGG | - | chr5.1:29214641-29214660 | MS.gene49571:CDS | 30.0% |
| ! | CTTTCTGATAAAGTTTTTGC+TGG | - | chr5.1:29214634-29214653 | MS.gene49571:CDS | 30.0% |
| AGAAAGAAGGAGACAATTTC+TGG | - | chr5.1:29214476-29214495 | MS.gene49571:CDS | 35.0% | |
| CAGAAATCGGTGAAATAGAA+TGG | - | chr5.1:29214512-29214531 | MS.gene49571:CDS | 35.0% | |
| CTTCAATTCCGATGCTAATT+CGG | + | chr5.1:29214434-29214453 | None:intergenic | 35.0% | |
| GAAATAGAATGGCCTGAATT+TGG | - | chr5.1:29214523-29214542 | MS.gene49571:CDS | 35.0% | |
| ! | CCTGAATTTGGGAAAGTTTT+GGG | - | chr5.1:29214535-29214554 | MS.gene49571:CDS | 35.0% |
| ! | GATAAAGTTTTTGCTGGCAA+AGG | - | chr5.1:29214640-29214659 | MS.gene49571:CDS | 35.0% |
| ! | TAAAGTTTTTGCTGGCAAAG+GGG | - | chr5.1:29214642-29214661 | MS.gene49571:CDS | 35.0% |
| AAGTGGAAGAATTGAGGACA+GGG | + | chr5.1:29214286-29214305 | None:intergenic | 40.0% | |
| CAAGAGAGGAAAGAGAAAGA+AGG | - | chr5.1:29214463-29214482 | MS.gene49571:CDS | 40.0% | |
| CCCAAAACTTTCCCAAATTC+AGG | + | chr5.1:29214538-29214557 | None:intergenic | 40.0% | |
| TGAAGAAAGCAATGCAAGAG+AGG | - | chr5.1:29214449-29214468 | MS.gene49571:CDS | 40.0% | |
| ! | GAAGGAGACAATTTCTGGAA+CGG | - | chr5.1:29214481-29214500 | MS.gene49571:CDS | 40.0% |
| ! | GCCTGAATTTGGGAAAGTTT+TGG | - | chr5.1:29214534-29214553 | MS.gene49571:CDS | 40.0% |
| ! | GGTTTGAAGTGGAAGAATTG+AGG | + | chr5.1:29214292-29214311 | None:intergenic | 40.0% |
| AACCGAGTCCGAATTAGCAT+CGG | - | chr5.1:29214423-29214442 | MS.gene49571:CDS | 45.0% | |
| AACGGCGTGGTTTCAGAAAT+CGG | - | chr5.1:29214499-29214518 | MS.gene49571:CDS | 45.0% | |
| AATTGAGGACAGGGAGTAGA+AGG | + | chr5.1:29214277-29214296 | None:intergenic | 45.0% | |
| AGAGTAAGAGAAGGGAGTGA+GGG | + | chr5.1:29214319-29214338 | None:intergenic | 45.0% | |
| GAAGTGGAAGAATTGAGGAC+AGG | + | chr5.1:29214287-29214306 | None:intergenic | 45.0% | |
| TTCCGATGCTAATTCGGACT+CGG | + | chr5.1:29214428-29214447 | None:intergenic | 45.0% | |
| ! | AATTCGGACTCGGTTGATTC+TGG | + | chr5.1:29214418-29214437 | None:intergenic | 45.0% |
| ! | AGAGAGTGTGAGTGTAAAGC+TGG | + | chr5.1:29214233-29214252 | None:intergenic | 45.0% |
| ! | GGTGGTGATGATGTAAAGTG+TGG | + | chr5.1:29214256-29214275 | None:intergenic | 45.0% |
| !! | GACTCGGTTGATTCTGGTTT+CGG | + | chr5.1:29214412-29214431 | None:intergenic | 45.0% |
| !! | GTTGATTCTGGTTTCGGTTC+TGG | + | chr5.1:29214406-29214425 | None:intergenic | 45.0% |
| !! | TTCTGGTTTCGGTTCTGGTT+CGG | + | chr5.1:29214401-29214420 | None:intergenic | 45.0% |
| !!! | GGGAAAGTTTTGGGTACAAC+TGG | - | chr5.1:29214544-29214563 | MS.gene49571:CDS | 45.0% |
| AAGAGAAGGGAGTGAGGGTT+TGG | + | chr5.1:29214314-29214333 | None:intergenic | 50.0% | |
| AGAGAAGGGAGTGAGGGTTT+GGG | + | chr5.1:29214313-29214332 | None:intergenic | 50.0% | |
| GAGAGTAAGAGAAGGGAGTG+AGG | + | chr5.1:29214320-29214339 | None:intergenic | 50.0% | |
| ! | GTGAGGGTTTGGGTTTGAAG+TGG | + | chr5.1:29214303-29214322 | None:intergenic | 50.0% |
| !! | AGACAATTTCTGGAACGGCG+TGG | - | chr5.1:29214486-29214505 | MS.gene49571:CDS | 50.0% |
| !! | TCTGGTTTCGGTTCTGGTTC+GGG | + | chr5.1:29214400-29214419 | None:intergenic | 50.0% |
| !!! | GGGTTGGTCTTTTTCCTCGA+CGG | + | chr5.1:29214368-29214387 | None:intergenic | 50.0% |
| GGCGCGTGAGAGTAAGAGAA+GGG | + | chr5.1:29214327-29214346 | None:intergenic | 55.0% | |
| TGAGGACAGGGAGTAGAAGG+TGG | + | chr5.1:29214274-29214293 | None:intergenic | 55.0% | |
| !! | CTGGTTTCGGTTCTGGTTCG+GGG | + | chr5.1:29214399-29214418 | None:intergenic | 55.0% |
| !! | TTTCGGTTCTGGTTCGGGGT+TGG | + | chr5.1:29214395-29214414 | None:intergenic | 55.0% |
| CGGCGCGTGAGAGTAAGAGA+AGG | + | chr5.1:29214328-29214347 | None:intergenic | 60.0% | |
| ! | TCTTTTTCCTCGACGGCGCG+TGG | + | chr5.1:29214361-29214380 | None:intergenic | 60.0% |
| !! | TCTGGTTCGGGGTTGGAGGT+GGG | + | chr5.1:29214388-29214407 | None:intergenic | 60.0% |
| !! | TTCTGGTTCGGGGTTGGAGG+TGG | + | chr5.1:29214389-29214408 | None:intergenic | 60.0% |
| GTTCGGGGTTGGAGGTGGGT+TGG | + | chr5.1:29214384-29214403 | None:intergenic | 65.0% | |
| ! | CGGCGCGTGGAAGTGTGAGT+CGG | + | chr5.1:29214348-29214367 | None:intergenic | 65.0% |
| !! | CGGTTCTGGTTCGGGGTTGG+AGG | + | chr5.1:29214392-29214411 | None:intergenic | 65.0% |
| CACACTTCCACGCGCCGTCG+AGG | - | chr5.1:29214351-29214370 | MS.gene49571:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 29214211 | 29214684 | 29214211 | ID=MS.gene49571 |
| chr5.1 | mRNA | 29214211 | 29214684 | 29214211 | ID=MS.gene49571.t1;Parent=MS.gene49571 |
| chr5.1 | exon | 29214211 | 29214684 | 29214211 | ID=MS.gene49571.t1.exon1;Parent=MS.gene49571.t1 |
| chr5.1 | CDS | 29214211 | 29214684 | 29214211 | ID=cds.MS.gene49571.t1;Parent=MS.gene49571.t1 |
| Gene Sequence |
| Protein sequence |
