AlfalfaGEDB | MS.gene49612

M. sativa cultivar XinJiangDaYe / MS.gene49612

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49612.t1	XP_003613544.1	87.2	141	3	1	1	141	1	126	2.80E-61	244.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49612.t1	Q38879	42.9	126	57	1	15	140	22	132	1.3e-27	124.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49612.t1	D8L5E0	87.2	141	3	1	1	141	1	126	2.0e-61	244.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene49612	0.804622	1.98E-49	-1.69E-46
MS.gene050971	MS.gene49612	0.818661	1.74E-52	-1.69E-46
MS.gene05316	MS.gene49612	0.809419	1.91E-50	-1.69E-46
MS.gene054486	MS.gene49612	0.800259	1.57E-48	-1.69E-46
MS.gene055909	MS.gene49612	0.802874	4.56E-49	-1.69E-46
MS.gene057609	MS.gene49612	0.923627	2.07E-89	-1.69E-46
MS.gene057610	MS.gene49612	0.939173	1.98E-99	-1.69E-46
MS.gene058007	MS.gene49612	0.811942	5.44E-51	-1.69E-46
MS.gene058072	MS.gene49612	0.805841	1.10E-49	-1.69E-46
MS.gene058075	MS.gene49612	0.830195	3.37E-55	-1.69E-46
MS.gene059674	MS.gene49612	0.814171	1.76E-51	-1.69E-46
MS.gene059807	MS.gene49612	0.831062	2.07E-55	-1.69E-46
MS.gene059876	MS.gene49612	0.831332	1.77E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49612.t1	MTR_5g037890	87.234	141	3	1	1	141	1	126	2.98e-87	250
MS.gene49612.t1	MTR_5g037950	59.574	141	41	2	1	140	1	126	6.84e-48	150
MS.gene49612.t1	MTR_5g037930	59.441	143	41	2	1	141	1	128	1.01e-47	150
MS.gene49612.t1	MTR_7g009070	50.413	121	45	1	20	140	28	133	4.63e-40	131
MS.gene49612.t1	MTR_3g112410	42.063	126	58	1	14	139	2	112	9.82e-33	112
MS.gene49612.t1	MTR_1g023140	38.281	128	64	1	14	141	2	114	5.36e-31	107
MS.gene49612.t1	MTR_5g021180	39.683	126	61	1	14	139	4	114	1.12e-30	107
MS.gene49612.t1	MTR_8g107460	36.975	119	60	1	18	136	254	357	7.31e-21	87.0
MS.gene49612.t1	MTR_7g073680	31.667	120	67	1	17	136	23	127	1.29e-19	79.3
MS.gene49612.t1	MTR_8g005320	30.000	120	69	1	17	136	25	129	1.50e-19	79.3
MS.gene49612.t1	MTR_2g010750	34.167	120	64	1	18	137	9	113	3.20e-19	77.8
MS.gene49612.t1	MTR_8g006685	31.667	120	67	2	17	136	94	198	2.15e-18	77.8
MS.gene49612.t1	MTR_8g006560	31.667	120	67	2	17	136	94	198	2.15e-18	77.8
MS.gene49612.t1	MTR_4g081380	32.787	122	63	3	18	137	10	114	2.06e-17	73.2
MS.gene49612.t1	MTR_2g082590	28.333	120	71	1	18	137	10	114	4.14e-16	69.7
MS.gene49612.t1	MTR_2g082590	29.688	128	66	3	10	137	24	127	6.68e-16	69.7
MS.gene49612.t1	MTR_7g080250	32.353	102	55	1	40	141	94	181	1.84e-13	64.3
MS.gene49612.t1	MTR_4g094690	27.966	118	72	2	21	138	62	166	9.43e-13	62.4
MS.gene49612.t1	MTR_1g098660	29.703	101	53	4	39	138	77	160	8.77e-12	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49612.t1	AT5G39950	42.857	126	57	1	15	140	22	132	6.19e-38	125
MS.gene49612.t1	AT1G69880	41.803	122	56	2	17	138	36	142	3.55e-31	109
MS.gene49612.t1	AT1G59730	37.063	143	72	2	1	140	1	128	7.34e-31	107
MS.gene49612.t1	AT3G51030	37.600	125	63	1	14	138	2	111	1.38e-29	104
MS.gene49612.t1	AT1G19730	42.063	126	57	2	14	138	2	112	1.56e-29	104
MS.gene49612.t1	AT1G45145	39.669	121	58	1	18	138	5	110	2.46e-28	101
MS.gene49612.t1	AT5G42980	38.710	124	61	1	18	141	5	113	3.39e-27	98.2
MS.gene49612.t1	AT3G17880	35.294	119	62	1	18	136	270	373	4.14e-19	82.4
MS.gene49612.t1	AT3G17880	35.294	119	62	1	18	136	263	366	4.26e-19	82.0
MS.gene49612.t1	AT3G56420	32.710	107	57	1	28	134	50	141	9.11e-18	75.1
MS.gene49612.t1	AT3G56420	32.710	107	57	1	28	134	50	141	9.11e-18	75.1
MS.gene49612.t1	AT3G56420	32.710	107	57	1	28	134	50	141	9.11e-18	75.1
MS.gene49612.t1	AT3G08710	29.365	126	72	2	11	136	18	126	1.06e-16	72.0
MS.gene49612.t1	AT3G08710	29.365	126	72	2	11	136	18	126	1.06e-16	72.0
MS.gene49612.t1	AT3G08710	29.365	126	72	2	11	136	18	126	1.06e-16	72.0
MS.gene49612.t1	AT1G11530	30.000	120	69	1	18	137	2	106	2.24e-15	67.8
MS.gene49612.t1	AT5G16400	35.294	102	52	2	40	141	97	184	1.39e-14	67.4
MS.gene49612.t1	AT3G02730	35.644	101	51	2	41	141	88	174	4.48e-14	65.9
MS.gene49612.t1	AT2G40790	28.947	114	66	1	28	141	50	148	1.88e-13	63.9
MS.gene49612.t1	AT2G40790	28.947	114	66	1	28	141	50	148	1.88e-13	63.9
MS.gene49612.t1	AT1G43560	27.273	132	76	5	1	127	33	149	1.61e-12	61.6
MS.gene49612.t1	AT4G03520	37.209	86	38	3	43	127	101	171	5.18e-12	60.8
MS.gene49612.t1	AT1G31020	27.206	136	76	3	6	138	41	156	2.25e-11	58.5
MS.gene49612.t1	AT1G03680	35.417	96	45	4	45	138	97	177	4.10e-11	58.2
MS.gene49612.t1	AT1G76760	27.273	110	58	5	33	135	70	164	9.00e-11	57.0

Find 28 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTCCATTCCACTGCTAAA+TGG	0.238150	5.1:-28560751	MS.gene49612:CDS
GCTTGATGTTGATGAGTTAT+TGG	0.264813	5.1:-28560502	MS.gene49612:intron
GCAGCAAAATCTTTCAAAAC+TGG	0.287096	5.1:+28560552	None:intergenic
TTATTGATTTCACAGCTAAA+TGG	0.322109	5.1:-28560599	MS.gene49612:CDS
CCCACAATATAATTGGCAAC+AGG	0.356350	5.1:-28560361	MS.gene49612:CDS
GGGAGTGTCTCAAACCTTCC+AGG	0.392325	5.1:-28560340	MS.gene49612:CDS
TAAAGTGGTGGGAGCAAGAA+AGG	0.432390	5.1:-28560268	MS.gene49612:CDS
CCTGTTGCCAATTATATTGT+GGG	0.436903	5.1:+28560361	None:intergenic
TTCTTAATGAGTAGAAAAGT+TGG	0.438267	5.1:+28560306	None:intergenic
CCCTGTTGCCAATTATATTG+TGG	0.464449	5.1:+28560360	None:intergenic
GTGTGGACCTTGCAAAGTTA+TGG	0.487071	5.1:-28560577	MS.gene49612:CDS
AGGAAAGGTTGTTGATAAAG+TGG	0.493130	5.1:-28560283	MS.gene49612:CDS
TAGCAGTGGAATGGAATGTA+AGG	0.498154	5.1:+28560756	None:intergenic
TCTACTCATTAAGAAAGGAA+AGG	0.517021	5.1:-28560298	MS.gene49612:CDS
ATACACAAAATTATACAAGT+TGG	0.527315	5.1:+28560808	None:intergenic
CATTCCATTTAGCAGTGGAA+TGG	0.530623	5.1:+28560747	None:intergenic
TGGAATGGAATGTAAGGACA+TGG	0.568326	5.1:+28560762	None:intergenic
AAGTTGGCAATGAATGCACC+TGG	0.579245	5.1:+28560322	None:intergenic
TGGCAATGAATGCACCTGGA+AGG	0.589602	5.1:+28560326	None:intergenic
GATTTCACAGCTAAATGGTG+TGG	0.590907	5.1:-28560594	MS.gene49612:CDS
AAGGTTGTTGATAAAGTGGT+GGG	0.601867	5.1:-28560279	MS.gene49612:CDS
CCACAATATAATTGGCAACA+GGG	0.601984	5.1:-28560360	MS.gene49612:CDS
ACTGGATCCATAACTTTGCA+AGG	0.608925	5.1:+28560570	None:intergenic
ATGAGCATTCCATTTAGCAG+TGG	0.609708	5.1:+28560742	None:intergenic
AAAGGTTGTTGATAAAGTGG+TGG	0.610487	5.1:-28560280	MS.gene49612:CDS
CAAGAAAGGAGGAACTACAA+AGG	0.696570	5.1:-28560254	MS.gene49612:CDS
AGTGGTGGGAGCAAGAAAGG+AGG	0.703427	5.1:-28560265	MS.gene49612:CDS
GGAATGGAATGTAAGGACAT+GGG	0.736550	5.1:+28560763	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAAAGAAACTAATAAAC+TGG	-	chr5.1:28560334-28560353	MS.gene49612:CDS	15.0%
!!	ATACACAAAATTATACAAGT+TGG	+	chr5.1:28560240-28560259	None:intergenic	20.0%
!!!	TCTTATGCATTATTTTTCTT+TGG	-	chr5.1:28560414-28560433	MS.gene49612:intron	20.0%
!	TAACAAACTAGTTAAAGTGA+AGG	+	chr5.1:28560584-28560603	None:intergenic	25.0%
!	TTCTTAATGAGTAGAAAAGT+TGG	+	chr5.1:28560742-28560761	None:intergenic	25.0%
!!	ACTTTTCTACTCATTAAGAA+AGG	-	chr5.1:28560742-28560761	MS.gene49612:CDS	25.0%
!!	TGTTTTTCCCACAATATAAT+TGG	-	chr5.1:28560677-28560696	MS.gene49612:intron	25.0%
!!!	TTATTGATTTCACAGCTAAA+TGG	-	chr5.1:28560446-28560465	MS.gene49612:intron	25.0%
!!!	TTATTTTTCTTTGGCATAGA+TGG	-	chr5.1:28560423-28560442	MS.gene49612:intron	25.0%
	TCTACTCATTAAGAAAGGAA+AGG	-	chr5.1:28560747-28560766	MS.gene49612:CDS	30.0%
	CCACAATATAATTGGCAACA+GGG	-	chr5.1:28560685-28560704	MS.gene49612:intron	35.0%
	CCTGTTGCCAATTATATTGT+GGG	+	chr5.1:28560687-28560706	None:intergenic	35.0%
	GCAGCAAAATCTTTCAAAAC+TGG	+	chr5.1:28560496-28560515	None:intergenic	35.0%
!	AAAGGTTGTTGATAAAGTGG+TGG	-	chr5.1:28560765-28560784	MS.gene49612:CDS	35.0%
!	AAGGTTGTTGATAAAGTGGT+GGG	-	chr5.1:28560766-28560785	MS.gene49612:CDS	35.0%
!	AGGAAAGGTTGTTGATAAAG+TGG	-	chr5.1:28560762-28560781	MS.gene49612:CDS	35.0%
!	GCTTGATGTTGATGAGTTAT+TGG	-	chr5.1:28560543-28560562	MS.gene49612:CDS	35.0%
	ACAAATCAGTTCTCAAGTGC+TGG	+	chr5.1:28560660-28560679	None:intergenic	40.0%
	ACTGGATCCATAACTTTGCA+AGG	+	chr5.1:28560478-28560497	None:intergenic	40.0%
	ATGAGCATTCCATTTAGCAG+TGG	+	chr5.1:28560306-28560325	None:intergenic	40.0%
	CAAGAAAGGAGGAACTACAA+AGG	-	chr5.1:28560791-28560810	MS.gene49612:CDS	40.0%
	CATTCCATTCCACTGCTAAA+TGG	-	chr5.1:28560294-28560313	MS.gene49612:CDS	40.0%
	CATTCCATTTAGCAGTGGAA+TGG	+	chr5.1:28560301-28560320	None:intergenic	40.0%
	CCCACAATATAATTGGCAAC+AGG	-	chr5.1:28560684-28560703	MS.gene49612:intron	40.0%
	CCCTGTTGCCAATTATATTG+TGG	+	chr5.1:28560688-28560707	None:intergenic	40.0%
	GATTTCACAGCTAAATGGTG+TGG	-	chr5.1:28560451-28560470	MS.gene49612:intron	40.0%
	GGAATGGAATGTAAGGACAT+GGG	+	chr5.1:28560285-28560304	None:intergenic	40.0%
	TAGCAGTGGAATGGAATGTA+AGG	+	chr5.1:28560292-28560311	None:intergenic	40.0%
	TGGAATGGAATGTAAGGACA+TGG	+	chr5.1:28560286-28560305	None:intergenic	40.0%
	AAGTTGGCAATGAATGCACC+TGG	+	chr5.1:28560726-28560745	None:intergenic	45.0%
	GTGTGGACCTTGCAAAGTTA+TGG	-	chr5.1:28560468-28560487	MS.gene49612:intron	45.0%
!	TAAAGTGGTGGGAGCAAGAA+AGG	-	chr5.1:28560777-28560796	MS.gene49612:CDS	45.0%
	TGGCAATGAATGCACCTGGA+AGG	+	chr5.1:28560722-28560741	None:intergenic	50.0%
!	AGTGGTGGGAGCAAGAAAGG+AGG	-	chr5.1:28560780-28560799	MS.gene49612:CDS	55.0%
!!	GGGAGTGTCTCAAACCTTCC+AGG	-	chr5.1:28560705-28560724	MS.gene49612:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	28560230	28560837	28560230	ID=MS.gene49612
chr5.1	mRNA	28560230	28560837	28560230	ID=MS.gene49612.t1;Parent=MS.gene49612
chr5.1	exon	28560712	28560837	28560712	ID=MS.gene49612.t1.exon1;Parent=MS.gene49612.t1
chr5.1	CDS	28560712	28560837	28560712	ID=cds.MS.gene49612.t1;Parent=MS.gene49612.t1
chr5.1	exon	28560503	28560625	28560503	ID=MS.gene49612.t1.exon2;Parent=MS.gene49612.t1
chr5.1	CDS	28560503	28560625	28560503	ID=cds.MS.gene49612.t1;Parent=MS.gene49612.t1
chr5.1	exon	28560230	28560406	28560230	ID=MS.gene49612.t1.exon3;Parent=MS.gene49612.t1
chr5.1	CDS	28560230	28560406	28560230	ID=cds.MS.gene49612.t1;Parent=MS.gene49612.t1

Gene Sequence

>MS.gene49612

ATGAGTTCCAACTTGTATAATTTTGTGTATGTTGATAGATCAACAGAATCATCCCATGTCCTTACATTCCATTCCACTGCTAAATGGAATGCTCATTTTGAAGCTTTTAAAGAAACTAATAAACTGGTATGTTTGTGAAGCTAAATTCTTAACTAGCTAGAGTCTTAGAAGATAAAATTAGTTTTCTTATGCATTATTTTTCTTTGGCATAGATGGTTATTGATTTCACAGCTAAATGGTGTGGACCTTGCAAAGTTATGGATCCAGTTTTGAAAGATTTTGCTGCAAAATACACTGATGTTGAGTTCATTAAGCTTGATGTTGATGAGTTATTGGTATGTTACAAATTATCCTTCACTTTAACTAGTTTGTTATTTAATTTTTTTTTTGAAAGAACTAGTTTGTTATTTAAAGTATAACATGATTTCCAGCACTTGAGAACTGATTTGTTTTTCCCACAATATAATTGGCAACAGGGAGTGTCTCAAACCTTCCAGGTGCATTCATTGCCAACTTTTCTACTCATTAAGAAAGGAAAGGTTGTTGATAAAGTGGTGGGAGCAAGAAAGGAGGAACTACAAAGGATGATTGAAAAACAAAGAAATTAA

Protein sequence

>MS.gene49612.t1

MSSNLYNFVYVDRSTESSHVLTFHSTAKWNAHFEAFKETNKLMVIDFTAKWCGPCKVMDPVLKDFAAKYTDVEFIKLDVDELLHLRTDLFFPQYNWQQGVSQTFQVHSLPTFLLIKKGKVVDKVVGARKEELQRMIEKQRN