Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058072.t1 | XP_003610789.2 | 44.8 | 534 | 13 | 1 | 1 | 252 | 306 | 839 | 1.70E-101 | 379 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058072.t1 | G7K528 | 44.8 | 534 | 13 | 1 | 1 | 252 | 306 | 839 | 1.2e-101 | 379.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058072 | MS.gene058075 | 0.874687 | 5.56E-68 | -1.69E-46 |
| MS.gene058072 | MS.gene059807 | 0.836636 | 8.33E-57 | -1.69E-46 |
| MS.gene058072 | MS.gene066009 | 0.819724 | 9.99E-53 | -1.69E-46 |
| MS.gene058072 | MS.gene21790 | 0.847459 | 1.14E-59 | -1.69E-46 |
| MS.gene058072 | MS.gene40499 | 0.892584 | 1.39E-74 | -1.69E-46 |
| MS.gene058072 | MS.gene44228 | 0.811159 | 8.05E-51 | -1.69E-46 |
| MS.gene058072 | MS.gene46957 | 0.80305 | 4.19E-49 | -1.69E-46 |
| MS.gene058072 | MS.gene49612 | 0.805841 | 1.10E-49 | -1.69E-46 |
| MS.gene058072 | MS.gene52997 | 0.811214 | 7.83E-51 | -1.69E-46 |
| MS.gene058072 | MS.gene71450 | 0.813184 | 2.91E-51 | -1.69E-46 |
| MS.gene058072 | MS.gene76535 | 0.818056 | 2.39E-52 | -1.69E-46 |
| MS.gene058072 | MS.gene85151 | 0.802938 | 4.42E-49 | -1.69E-46 |
| MS.gene058072 | MS.gene89675 | 0.810194 | 1.30E-50 | -1.69E-46 |
| MS.gene058072 | MS.gene99874 | 0.825452 | 4.65E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058072.t1 | MTR_5g007040 | 90.789 | 152 | 14 | 0 | 101 | 252 | 688 | 839 | 3.60e-89 | 282 |
| MS.gene058072.t1 | MTR_5g007040 | 92.105 | 114 | 9 | 0 | 1 | 114 | 306 | 419 | 1.95e-67 | 224 |
| MS.gene058072.t1 | MTR_6g046940 | 74.561 | 114 | 9 | 2 | 1 | 114 | 276 | 369 | 1.21e-46 | 164 |
| MS.gene058072.t1 | MTR_6g046940 | 98.305 | 59 | 1 | 0 | 116 | 174 | 615 | 673 | 2.02e-30 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058072.t1 | AT5G43630 | 56.250 | 128 | 54 | 2 | 126 | 252 | 665 | 791 | 9.83e-38 | 140 |
| MS.gene058072.t1 | AT5G43630 | 56.250 | 128 | 54 | 2 | 126 | 252 | 665 | 791 | 9.83e-38 | 140 |
| MS.gene058072.t1 | AT5G43630 | 56.250 | 128 | 54 | 2 | 126 | 252 | 665 | 791 | 9.83e-38 | 140 |
| MS.gene058072.t1 | AT5G43630 | 56.250 | 128 | 54 | 2 | 126 | 252 | 665 | 791 | 9.83e-38 | 140 |
Find 52 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTCTGGAAAACAGGTTAT+TGG | 0.246005 | 5.1:-1635614 | None:intergenic |
| AACCATGCATTATGAAGATT+TGG | 0.280576 | 5.1:-1635401 | None:intergenic |
| TTGCATGATGCATTGCATTC+TGG | 0.295405 | 5.1:-1635677 | None:intergenic |
| TGCGCCTGCGGCTTGGGAAG+TGG | 0.328935 | 5.1:+1636053 | MS.gene058072:CDS |
| CTTCTCAAAACTATGTTTCT+CGG | 0.355144 | 5.1:-1636253 | None:intergenic |
| ATTGAAAATGCCGTTTGTTA+AGG | 0.362224 | 5.1:-1635643 | None:intergenic |
| TACCTCAAGAAAATCTTGAT+TGG | 0.368233 | 5.1:-1636343 | None:intergenic |
| CGGCTTGGGAAGTGGGAGGA+AGG | 0.403364 | 5.1:+1636061 | MS.gene058072:CDS |
| TATCCAAGTTTGAAGAATAT+AGG | 0.406340 | 5.1:+1635509 | MS.gene058072:CDS |
| GCAATTGATCTCTGGAAAAC+AGG | 0.406472 | 5.1:-1635621 | None:intergenic |
| GCGCCTGCGGCTTGGGAAGT+GGG | 0.408888 | 5.1:+1636054 | MS.gene058072:CDS |
| AAGTGGGAGGAAGGGCTTGG+AGG | 0.415944 | 5.1:+1636070 | MS.gene058072:CDS |
| TATAACATCTCCGTCGAGAT+TGG | 0.435268 | 5.1:+1635751 | MS.gene058072:CDS |
| TTGTTAAGGCAATTGATCTC+TGG | 0.437536 | 5.1:-1635629 | None:intergenic |
| GGGAAGTGGGAGGAAGGGCT+TGG | 0.440847 | 5.1:+1636067 | MS.gene058072:CDS |
| CTCGACGGAGATGTTATACT+AGG | 0.443437 | 5.1:-1635746 | None:intergenic |
| GAGGAAGGGCTTGGAGGAAC+TGG | 0.447576 | 5.1:+1636076 | MS.gene058072:CDS |
| AATCTCTCTCAGTGAAAGTA+GGG | 0.456729 | 5.1:+1636286 | MS.gene058072:CDS |
| GTTCCTATATTCTTCAAACT+TGG | 0.462575 | 5.1:-1635512 | None:intergenic |
| CCAAGTGATAACACGGTACA+TGG | 0.465869 | 5.1:+1635578 | MS.gene058072:CDS |
| AAATCTCTCTCAGTGAAAGT+AGG | 0.468291 | 5.1:+1636285 | MS.gene058072:CDS |
| CTTCAAACTTGGATAATACA+TGG | 0.480740 | 5.1:-1635501 | None:intergenic |
| CCATGTACCGTGTTATCACT+TGG | 0.497567 | 5.1:-1635578 | None:intergenic |
| CCTCCCACTTCCCAAGCCGC+AGG | 0.500679 | 5.1:-1636057 | None:intergenic |
| TATGAAGATTTGGATTTGCA+AGG | 0.503391 | 5.1:-1635391 | None:intergenic |
| ATCCAAATCTTCATAATGCA+TGG | 0.507310 | 5.1:+1635399 | MS.gene058072:CDS |
| TTACTTCAGGATGTCGGTGC+GGG | 0.508749 | 5.1:-1635837 | None:intergenic |
| GGCTTGGGAAGTGGGAGGAA+GGG | 0.512560 | 5.1:+1636062 | MS.gene058072:CDS |
| GGATTTCAAGCATGGTGAAA+GGG | 0.521068 | 5.1:+1635324 | None:intergenic |
| ACAAGAATGTTTCATCAAGA+AGG | 0.536595 | 5.1:+1635533 | MS.gene058072:CDS |
| AGATCAATTGCCTTAACAAA+CGG | 0.537737 | 5.1:+1635633 | MS.gene058072:CDS |
| TGGATTTCAAGCATGGTGAA+AGG | 0.544832 | 5.1:+1635323 | None:intergenic |
| CATTACTTACTTCAGGATGT+CGG | 0.547841 | 5.1:-1635843 | None:intergenic |
| GTGGCTTGTATAACTATAAG+TGG | 0.554774 | 5.1:+1636106 | MS.gene058072:CDS |
| ATACTGTGGTTCTAGTTCAA+AGG | 0.562249 | 5.1:+1635703 | MS.gene058072:CDS |
| TGGATCAATCTCGCAACTCG+AGG | 0.580905 | 5.1:+1636021 | MS.gene058072:CDS |
| CGAGTTGCGAGATTGATCCA+TGG | 0.583492 | 5.1:-1636018 | None:intergenic |
| GAGTTGCGAGATTGATCCAT+GGG | 0.584749 | 5.1:-1636017 | None:intergenic |
| GGATCAATCTCGCAACTCGA+GGG | 0.607948 | 5.1:+1636022 | MS.gene058072:CDS |
| CCTGCGGCTTGGGAAGTGGG+AGG | 0.612188 | 5.1:+1636057 | MS.gene058072:CDS |
| ACCAGATGGATAACTGCCCA+TGG | 0.618765 | 5.1:+1636001 | MS.gene058072:intron |
| ATGTTTCATCAAGAAGGAGA+AGG | 0.623506 | 5.1:+1635539 | MS.gene058072:CDS |
| GAAGATTTCACATGTGCCAG+AGG | 0.625270 | 5.1:+1635781 | MS.gene058072:CDS |
| ATCTCTCTCAGTGAAAGTAG+GGG | 0.638733 | 5.1:+1636287 | MS.gene058072:CDS |
| AAGATTTCACATGTGCCAGA+GGG | 0.642256 | 5.1:+1635782 | MS.gene058072:CDS |
| TCTCTCTCAGTGAAAGTAGG+GGG | 0.646665 | 5.1:+1636288 | MS.gene058072:CDS |
| TGGAGGAACTGGTTACCATG+TGG | 0.653299 | 5.1:+1636087 | MS.gene058072:CDS |
| CTTACTTCAGGATGTCGGTG+CGG | 0.670580 | 5.1:-1635838 | None:intergenic |
| TCAAGAAGGAGAAGGTGACG+AGG | 0.679529 | 5.1:+1635547 | MS.gene058072:CDS |
| GCATCATGCAAGAAATACTG+TGG | 0.685241 | 5.1:+1635689 | MS.gene058072:CDS |
| TTTAGAGCCAAGTGATAACA+CGG | 0.691371 | 5.1:+1635571 | MS.gene058072:CDS |
| TTATAGTTATACAAGCCACA+TGG | 0.755776 | 5.1:-1636102 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATCATCAATGATTATTTTT+TGG | + | chr5.1:1636372-1636391 | MS.gene058072:intron | 15.0% |
| !!! | TGGATTTAATTTGTATATAT+TGG | + | chr5.1:1636392-1636411 | MS.gene058072:intron | 15.0% |
| !!! | TTTTAAACTTTTTCTTATTC+TGG | + | chr5.1:1636217-1636236 | MS.gene058072:intron | 15.0% |
| !! | ATTTCATCCTTAATGTAATT+GGG | - | chr5.1:1635926-1635945 | None:intergenic | 20.0% |
| !! | TATTTCATCCTTAATGTAAT+TGG | - | chr5.1:1635927-1635946 | None:intergenic | 20.0% |
| ! | TATCCAAGTTTGAAGAATAT+AGG | + | chr5.1:1635509-1635528 | MS.gene058072:CDS | 25.0% |
| !! | ATAATACATGGACTGAAAAA+TGG | - | chr5.1:1635492-1635511 | None:intergenic | 25.0% |
| !! | GATTTGAATCCTGTATTTTT+CGG | - | chr5.1:1635470-1635489 | None:intergenic | 25.0% |
| !!! | ATAGCTGGTTTAATTGTTTT+TGG | + | chr5.1:1636168-1636187 | MS.gene058072:intron | 25.0% |
| AACCATGCATTATGAAGATT+TGG | - | chr5.1:1635404-1635423 | None:intergenic | 30.0% | |
| ACAAGAATGTTTCATCAAGA+AGG | + | chr5.1:1635533-1635552 | MS.gene058072:CDS | 30.0% | |
| AGATCAATTGCCTTAACAAA+CGG | + | chr5.1:1635633-1635652 | MS.gene058072:CDS | 30.0% | |
| ATCCAAATCTTCATAATGCA+TGG | + | chr5.1:1635399-1635418 | MS.gene058072:CDS | 30.0% | |
| CTTCAAACTTGGATAATACA+TGG | - | chr5.1:1635504-1635523 | None:intergenic | 30.0% | |
| CTTCTCAAAACTATGTTTCT+CGG | - | chr5.1:1636256-1636275 | None:intergenic | 30.0% | |
| GTTCCTATATTCTTCAAACT+TGG | - | chr5.1:1635515-1635534 | None:intergenic | 30.0% | |
| TACCTCAAGAAAATCTTGAT+TGG | - | chr5.1:1636346-1636365 | None:intergenic | 30.0% | |
| TATGAAGATTTGGATTTGCA+AGG | - | chr5.1:1635394-1635413 | None:intergenic | 30.0% | |
| TCTATTGCCCAATTACATTA+AGG | + | chr5.1:1635916-1635935 | MS.gene058072:intron | 30.0% | |
| TTATAGTTATACAAGCCACA+TGG | - | chr5.1:1636105-1636124 | None:intergenic | 30.0% | |
| ! | ATCCAATCAAGATTTTCTTG+AGG | + | chr5.1:1636341-1636360 | MS.gene058072:CDS | 30.0% |
| ! | ATTGAAAATGCCGTTTGTTA+AGG | - | chr5.1:1635646-1635665 | None:intergenic | 30.0% |
| AAATCTCTCTCAGTGAAAGT+AGG | + | chr5.1:1636285-1636304 | MS.gene058072:CDS | 35.0% | |
| AATCTCTCTCAGTGAAAGTA+GGG | + | chr5.1:1636286-1636305 | MS.gene058072:CDS | 35.0% | |
| AATGTCACAGCATAAGTTAG+TGG | - | chr5.1:1635874-1635893 | None:intergenic | 35.0% | |
| AGCTATGATTCTACATTCCA+AGG | - | chr5.1:1636154-1636173 | None:intergenic | 35.0% | |
| ATGTCACAGCATAAGTTAGT+GGG | - | chr5.1:1635873-1635892 | None:intergenic | 35.0% | |
| ATGTTTCATCAAGAAGGAGA+AGG | + | chr5.1:1635539-1635558 | MS.gene058072:CDS | 35.0% | |
| CATTACTTACTTCAGGATGT+CGG | - | chr5.1:1635846-1635865 | None:intergenic | 35.0% | |
| GCTATGATTCTACATTCCAA+GGG | - | chr5.1:1636153-1636172 | None:intergenic | 35.0% | |
| TTGGAATGTAGAATCATAGC+TGG | + | chr5.1:1636153-1636172 | MS.gene058072:intron | 35.0% | |
| TTGTTAAGGCAATTGATCTC+TGG | - | chr5.1:1635632-1635651 | None:intergenic | 35.0% | |
| ! | ATCTCTGGAAAACAGGTTAT+TGG | - | chr5.1:1635617-1635636 | None:intergenic | 35.0% |
| ! | GTGGCTTGTATAACTATAAG+TGG | + | chr5.1:1636106-1636125 | MS.gene058072:CDS | 35.0% |
| ! | TTTAGAGCCAAGTGATAACA+CGG | + | chr5.1:1635571-1635590 | MS.gene058072:CDS | 35.0% |
| ! | TTTCACTGAGAGAGATTTTC+TGG | - | chr5.1:1636283-1636302 | None:intergenic | 35.0% |
| !! | ATACTGTGGTTCTAGTTCAA+AGG | + | chr5.1:1635703-1635722 | MS.gene058072:CDS | 35.0% |
| AAGATTTCACATGTGCCAGA+GGG | + | chr5.1:1635782-1635801 | MS.gene058072:CDS | 40.0% | |
| AATCCTGAGCCGAAAAATAC+AGG | + | chr5.1:1635458-1635477 | MS.gene058072:CDS | 40.0% | |
| ACTGCATCAAAAATTCCCTC+TGG | - | chr5.1:1635800-1635819 | None:intergenic | 40.0% | |
| ATCTCTCTCAGTGAAAGTAG+GGG | + | chr5.1:1636287-1636306 | MS.gene058072:CDS | 40.0% | |
| GCATCATGCAAGAAATACTG+TGG | + | chr5.1:1635689-1635708 | MS.gene058072:CDS | 40.0% | |
| TACAGTTAGTGTGTACCAGA+TGG | + | chr5.1:1635987-1636006 | MS.gene058072:intron | 40.0% | |
| TATAACATCTCCGTCGAGAT+TGG | + | chr5.1:1635751-1635770 | MS.gene058072:CDS | 40.0% | |
| TTAAAATCACACCTTGCCCT+TGG | + | chr5.1:1636134-1636153 | MS.gene058072:intron | 40.0% | |
| TTGCATGATGCATTGCATTC+TGG | - | chr5.1:1635680-1635699 | None:intergenic | 40.0% | |
| ! | GCAATTGATCTCTGGAAAAC+AGG | - | chr5.1:1635624-1635643 | None:intergenic | 40.0% |
| ! | TTCTTTTCAGCCAATCTCGA+CGG | - | chr5.1:1635764-1635783 | None:intergenic | 40.0% |
| !! | AATCCTGTATTTTTCGGCTC+AGG | - | chr5.1:1635464-1635483 | None:intergenic | 40.0% |
| AGTAGGGGGAATCAAGTGTA+TGG | + | chr5.1:1636302-1636321 | MS.gene058072:CDS | 45.0% | |
| CCAAGTGATAACACGGTACA+TGG | + | chr5.1:1635578-1635597 | MS.gene058072:CDS | 45.0% | |
| CCATGTACCGTGTTATCACT+TGG | - | chr5.1:1635581-1635600 | None:intergenic | 45.0% | |
| CTCGACGGAGATGTTATACT+AGG | - | chr5.1:1635749-1635768 | None:intergenic | 45.0% | |
| GAAGATTTCACATGTGCCAG+AGG | + | chr5.1:1635781-1635800 | MS.gene058072:CDS | 45.0% | |
| GAGTTGCGAGATTGATCCAT+GGG | - | chr5.1:1636020-1636039 | None:intergenic | 45.0% | |
| GATTCTACATTCCAAGGGCA+AGG | - | chr5.1:1636148-1636167 | None:intergenic | 45.0% | |
| GGGCTTGCATTACTTACTTC+AGG | - | chr5.1:1635853-1635872 | None:intergenic | 45.0% | |
| TCTCTCTCAGTGAAAGTAGG+GGG | + | chr5.1:1636288-1636307 | MS.gene058072:CDS | 45.0% | |
| ACCAGATGGATAACTGCCCA+TGG | + | chr5.1:1636001-1636020 | MS.gene058072:intron | 50.0% | |
| CGAGTTGCGAGATTGATCCA+TGG | - | chr5.1:1636021-1636040 | None:intergenic | 50.0% | |
| CTTACTTCAGGATGTCGGTG+CGG | - | chr5.1:1635841-1635860 | None:intergenic | 50.0% | |
| GGATCAATCTCGCAACTCGA+GGG | + | chr5.1:1636022-1636041 | MS.gene058072:CDS | 50.0% | |
| TCAAGAAGGAGAAGGTGACG+AGG | + | chr5.1:1635547-1635566 | MS.gene058072:CDS | 50.0% | |
| TCCATGGGCAGTTATCCATC+TGG | - | chr5.1:1636005-1636024 | None:intergenic | 50.0% | |
| TGGAGGAACTGGTTACCATG+TGG | + | chr5.1:1636087-1636106 | MS.gene058072:CDS | 50.0% | |
| TGGATCAATCTCGCAACTCG+AGG | + | chr5.1:1636021-1636040 | MS.gene058072:CDS | 50.0% | |
| TTACTTCAGGATGTCGGTGC+GGG | - | chr5.1:1635840-1635859 | None:intergenic | 50.0% | |
| !!! | AGGGTTTTTTCTTGCGCCTG+CGG | + | chr5.1:1636041-1636060 | MS.gene058072:CDS | 50.0% |
| ! | TTTTCTTGCGCCTGCGGCTT+GGG | + | chr5.1:1636047-1636066 | MS.gene058072:CDS | 55.0% |
| ! | TTTTTCTTGCGCCTGCGGCT+TGG | + | chr5.1:1636046-1636065 | MS.gene058072:CDS | 55.0% |
| AAGTGGGAGGAAGGGCTTGG+AGG | + | chr5.1:1636070-1636089 | MS.gene058072:CDS | 60.0% | |
| GAGGAAGGGCTTGGAGGAAC+TGG | + | chr5.1:1636076-1636095 | MS.gene058072:CDS | 60.0% | |
| GGCTTGGGAAGTGGGAGGAA+GGG | + | chr5.1:1636062-1636081 | MS.gene058072:CDS | 60.0% | |
| GGGAAGTGGGAGGAAGGGCT+TGG | + | chr5.1:1636067-1636086 | MS.gene058072:CDS | 65.0% | |
| ! | CGGCTTGGGAAGTGGGAGGA+AGG | + | chr5.1:1636061-1636080 | MS.gene058072:CDS | 65.0% |
| CCTCCCACTTCCCAAGCCGC+AGG | - | chr5.1:1636060-1636079 | None:intergenic | 70.0% | |
| ! | CCTGCGGCTTGGGAAGTGGG+AGG | + | chr5.1:1636057-1636076 | MS.gene058072:CDS | 70.0% |
| ! | GCGCCTGCGGCTTGGGAAGT+GGG | + | chr5.1:1636054-1636073 | MS.gene058072:CDS | 70.0% |
| ! | TGCGCCTGCGGCTTGGGAAG+TGG | + | chr5.1:1636053-1636072 | MS.gene058072:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 1635335 | 1636455 | 1635335 | ID=MS.gene058072 |
| chr5.1 | mRNA | 1635335 | 1636455 | 1635335 | ID=MS.gene058072.t1;Parent=MS.gene058072 |
| chr5.1 | exon | 1635335 | 1635855 | 1635335 | ID=MS.gene058072.t1.exon1;Parent=MS.gene058072.t1 |
| chr5.1 | CDS | 1635335 | 1635855 | 1635335 | ID=cds.MS.gene058072.t1;Parent=MS.gene058072.t1 |
| chr5.1 | exon | 1636006 | 1636127 | 1636006 | ID=MS.gene058072.t1.exon2;Parent=MS.gene058072.t1 |
| chr5.1 | CDS | 1636006 | 1636127 | 1636006 | ID=cds.MS.gene058072.t1;Parent=MS.gene058072.t1 |
| chr5.1 | exon | 1636250 | 1636362 | 1636250 | ID=MS.gene058072.t1.exon3;Parent=MS.gene058072.t1 |
| chr5.1 | CDS | 1636250 | 1636362 | 1636250 | ID=cds.MS.gene058072.t1;Parent=MS.gene058072.t1 |
| chr5.1 | exon | 1636447 | 1636455 | 1636447 | ID=MS.gene058072.t1.exon4;Parent=MS.gene058072.t1 |
| chr5.1 | CDS | 1636447 | 1636455 | 1636447 | ID=cds.MS.gene058072.t1;Parent=MS.gene058072.t1 |
| Gene Sequence |
| Protein sequence |
