Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50125.t1 | XP_024641057.1 | 99.6 | 255 | 1 | 0 | 1 | 255 | 1 | 255 | 6.20E-128 | 466.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50125.t1 | Q8W487 | 58.8 | 194 | 74 | 3 | 63 | 254 | 60 | 249 | 1.8e-46 | 187.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50125.t1 | A0A396I021 | 99.6 | 255 | 1 | 0 | 1 | 255 | 1 | 255 | 4.5e-128 | 466.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene000604 | MS.gene50125 | PPI |
| MS.gene055201 | MS.gene50125 | PPI |
| MS.gene46104 | MS.gene50125 | PPI |
| MS.gene46105 | MS.gene50125 | PPI |
| MS.gene040272 | MS.gene50125 | PPI |
| MS.gene89319 | MS.gene50125 | PPI |
| MS.gene053230 | MS.gene50125 | PPI |
| MS.gene034255 | MS.gene50125 | PPI |
| MS.gene040271 | MS.gene50125 | PPI |
| MS.gene67544 | MS.gene50125 | PPI |
| MS.gene040269 | MS.gene50125 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50125.t1 | MTR_5g080880 | 99.606 | 254 | 1 | 0 | 1 | 254 | 1 | 254 | 0.0 | 523 |
| MS.gene50125.t1 | MTR_5g080870 | 66.667 | 255 | 50 | 6 | 1 | 255 | 1 | 220 | 4.20e-110 | 321 |
| MS.gene50125.t1 | MTR_7g063520 | 48.400 | 250 | 110 | 5 | 8 | 254 | 20 | 253 | 3.09e-72 | 221 |
| MS.gene50125.t1 | MTR_4g006560 | 40.881 | 159 | 87 | 3 | 97 | 254 | 83 | 235 | 7.87e-31 | 114 |
| MS.gene50125.t1 | MTR_1g013120 | 32.500 | 240 | 142 | 9 | 24 | 254 | 4 | 232 | 5.63e-26 | 102 |
| MS.gene50125.t1 | MTR_5g093000 | 32.164 | 171 | 90 | 7 | 92 | 253 | 59 | 212 | 3.16e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50125.t1 | AT3G55605 | 50.000 | 270 | 106 | 8 | 1 | 254 | 1 | 257 | 1.78e-70 | 217 |
| MS.gene50125.t1 | AT5G05990 | 46.212 | 264 | 120 | 5 | 5 | 254 | 3 | 258 | 6.42e-70 | 216 |
| MS.gene50125.t1 | AT2G39795 | 53.299 | 197 | 86 | 3 | 60 | 254 | 57 | 249 | 1.41e-64 | 202 |
| MS.gene50125.t1 | AT5G02050 | 43.750 | 272 | 129 | 5 | 1 | 254 | 1 | 266 | 8.38e-59 | 187 |
| MS.gene50125.t1 | AT2G39790 | 35.249 | 261 | 138 | 6 | 1 | 253 | 1 | 238 | 1.48e-38 | 135 |
| MS.gene50125.t1 | AT1G15870 | 33.824 | 204 | 121 | 5 | 54 | 254 | 48 | 240 | 1.13e-26 | 103 |
| MS.gene50125.t1 | AT1G80720 | 31.863 | 204 | 125 | 6 | 54 | 254 | 48 | 240 | 6.75e-22 | 91.3 |
| MS.gene50125.t1 | AT1G80720 | 32.105 | 190 | 115 | 6 | 68 | 254 | 10 | 188 | 9.62e-22 | 89.7 |
| MS.gene50125.t1 | AT4G31930 | 32.632 | 190 | 114 | 7 | 68 | 253 | 53 | 232 | 7.29e-21 | 88.6 |
| MS.gene50125.t1 | AT4G32605 | 32.927 | 164 | 91 | 5 | 97 | 253 | 66 | 217 | 1.14e-17 | 79.7 |
Find 56 sgRNAs with CRISPR-Local
Find 230 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTATCAACTATCTTAAA+TGG | 0.175868 | 5.4:-63310692 | None:intergenic |
| TCCACAAGTACTTAGAAATA+AGG | 0.241404 | 5.4:+63311458 | MS.gene50125:CDS |
| CACATGCCTGATTTGGTTAC+TGG | 0.250062 | 5.4:+63310776 | MS.gene50125:CDS |
| AGTGTTATTGTCTGATTTCC+TGG | 0.252098 | 5.4:-63310713 | None:intergenic |
| ATCAGACAATAACACTTAAA+AGG | 0.289378 | 5.4:+63310720 | MS.gene50125:CDS |
| TAAGAAGGATGGGCCCTCTT+TGG | 0.320568 | 5.4:+63310886 | MS.gene50125:CDS |
| CCACAAGTACTTAGAAATAA+GGG | 0.328236 | 5.4:+63311459 | MS.gene50125:CDS |
| TGAGGTTCACATGCCTGATT+TGG | 0.354382 | 5.4:+63310769 | MS.gene50125:CDS |
| CTGTCGATAACAATCTCATC+TGG | 0.355760 | 5.4:-63310929 | None:intergenic |
| CCAAGTCAAGCACTTTCGTT+AGG | 0.373305 | 5.4:+63308074 | MS.gene50125:CDS |
| AGAAGTACATACTGAAAATC+TGG | 0.385204 | 5.4:-63311004 | None:intergenic |
| GCTCAGGAAACCGATGATTT+CGG | 0.391310 | 5.4:+63308283 | MS.gene50125:CDS |
| ATCTTAAATGGGAAATTGCT+TGG | 0.407574 | 5.4:-63310680 | None:intergenic |
| CCCTTATTTCTAAGTACTTG+TGG | 0.408675 | 5.4:-63311459 | None:intergenic |
| CGCCGCCGTCAATCGATACC+AGG | 0.411836 | 5.4:+63308159 | MS.gene50125:CDS |
| TTCAGTCAGTGTTTCTAAGA+AGG | 0.415683 | 5.4:+63310871 | MS.gene50125:CDS |
| TGCAAGAAATTTGTTGTGCT+TGG | 0.436674 | 5.4:-63311490 | None:intergenic |
| TGAAAATCTGGCCCGTCATA+AGG | 0.439441 | 5.4:-63310992 | None:intergenic |
| CACGGCGCGATGACCCGGTT+GGG | 0.442829 | 5.4:-63308131 | None:intergenic |
| TCTTCTCCGTTTCATCGAAT+CGG | 0.447658 | 5.4:+63308249 | MS.gene50125:CDS |
| CAAGGATAGTAGAGAATACT+TGG | 0.453079 | 5.4:+63311528 | MS.gene50125:CDS |
| GATTATCAACTATCTTAAAT+GGG | 0.456111 | 5.4:-63310691 | None:intergenic |
| TCTTCACCAGTAACCAAATC+AGG | 0.463223 | 5.4:-63310782 | None:intergenic |
| TTAGAAACACTGACTGAAAG+TGG | 0.463235 | 5.4:-63310866 | None:intergenic |
| GTCAGTGTTTCTAAGAAGGA+TGG | 0.492712 | 5.4:+63310875 | MS.gene50125:CDS |
| ATCGGAAATCACTTGTGCTC+AGG | 0.496436 | 5.4:+63308267 | MS.gene50125:CDS |
| TGATCATCAAGATGATGATA+AGG | 0.496458 | 5.4:+63310808 | MS.gene50125:CDS |
| TTTAAGATAGTTGATAATCC+AGG | 0.504200 | 5.4:+63310695 | MS.gene50125:CDS |
| CAAGTCAAGCACTTTCGTTA+GGG | 0.512325 | 5.4:+63308075 | MS.gene50125:CDS |
| GAAAGCACGGCGCGATGACC+CGG | 0.517251 | 5.4:-63308136 | None:intergenic |
| TGATTTCCGATTCGATGAAA+CGG | 0.520694 | 5.4:-63308255 | None:intergenic |
| ACTTACAGCGCCGAAATCAT+CGG | 0.522056 | 5.4:-63308293 | None:intergenic |
| GAGAAGAATATCATCGGAGG+TGG | 0.524675 | 5.4:-63308233 | None:intergenic |
| ACACTTAAAAGGACTTATCA+AGG | 0.527097 | 5.4:+63310731 | MS.gene50125:CDS |
| ATGAACAGATTCCTTATGAC+GGG | 0.541918 | 5.4:+63310981 | MS.gene50125:CDS |
| ATTGACGGCGGCGGAAAGCA+CGG | 0.549833 | 5.4:-63308149 | None:intergenic |
| TGAACCCTGGTATCGATTGA+CGG | 0.565956 | 5.4:-63308164 | None:intergenic |
| AGGTGAAGAAATTCAAGTTG+AGG | 0.571505 | 5.4:+63310751 | MS.gene50125:CDS |
| GCACGGCGCGATGACCCGGT+TGG | 0.590649 | 5.4:-63308132 | None:intergenic |
| TGTCGATAACAATCTCATCT+GGG | 0.591480 | 5.4:-63310928 | None:intergenic |
| CCTAACGAAAGTGCTTGACT+TGG | 0.591991 | 5.4:-63308074 | None:intergenic |
| CTGGTATCGATTGACGGCGG+CGG | 0.595525 | 5.4:-63308158 | None:intergenic |
| TCAGTGTTTCTAAGAAGGAT+GGG | 0.595788 | 5.4:+63310876 | MS.gene50125:CDS |
| CACAAGTACTTAGAAATAAG+GGG | 0.598764 | 5.4:+63311460 | MS.gene50125:CDS |
| ACACAACTGAACTCCAAAGA+GGG | 0.618264 | 5.4:-63310899 | None:intergenic |
| GAAACGGAGAAGAATATCAT+CGG | 0.632263 | 5.4:-63308239 | None:intergenic |
| GATGAACAGATTCCTTATGA+CGG | 0.641036 | 5.4:+63310980 | MS.gene50125:CDS |
| ATAGTAGAGAATACTTGGTG+TGG | 0.641539 | 5.4:+63311533 | MS.gene50125:CDS |
| AACACAACTGAACTCCAAAG+AGG | 0.648826 | 5.4:-63310900 | None:intergenic |
| GCCGCCGTCAATCGATACCA+GGG | 0.649914 | 5.4:+63308160 | MS.gene50125:CDS |
| ACAGAAGAAAAGTGAAACGT+AGG | 0.650656 | 5.4:-63308193 | None:intergenic |
| AGAAGAATATCATCGGAGGT+GGG | 0.660614 | 5.4:-63308232 | None:intergenic |
| ACGTAGGAACAAATGAACCC+TGG | 0.662093 | 5.4:-63308177 | None:intergenic |
| ACGGAGAAGAATATCATCGG+AGG | 0.705754 | 5.4:-63308236 | None:intergenic |
| GCATGAGTACATGATCAACA+AGG | 0.729521 | 5.4:+63311510 | MS.gene50125:CDS |
| ACCCTGGTATCGATTGACGG+CGG | 0.780259 | 5.4:-63308161 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGTTGAAAATTTGAATT+TGG | + | chr5.4:63310581-63310600 | MS.gene50125:intron | 15.0% |
| !! | AAGAGTAAAAAAAATTATCT+TGG | + | chr5.4:63309839-63309858 | MS.gene50125:intron | 15.0% |
| !! | TAAATATGATTATCTTAGTT+TGG | + | chr5.4:63309459-63309478 | MS.gene50125:intron | 15.0% |
| !! | TGAAATGAAACTTATTATTA+AGG | - | chr5.4:63310212-63310231 | None:intergenic | 15.0% |
| !! | TTATTTATGAAGAAAGAATT+TGG | + | chr5.4:63309317-63309336 | MS.gene50125:intron | 15.0% |
| !!! | AAATGTTGAAAAAATGATTT+TGG | + | chr5.4:63310636-63310655 | MS.gene50125:intron | 15.0% |
| !!! | ACAAAATTTTTAAAATAGAC+AGG | - | chr5.4:63309695-63309714 | None:intergenic | 15.0% |
| !!! | CAAAATTTTTAAAATAGACA+GGG | - | chr5.4:63309694-63309713 | None:intergenic | 15.0% |
| !!! | CTTTTACTCTATTAATTTAA+TGG | + | chr5.4:63309890-63309909 | MS.gene50125:intron | 15.0% |
| !! | ATGATGAGTCTAATAAATTA+AGG | - | chr5.4:63308327-63308346 | None:intergenic | 20.0% |
| !! | ATTATAATAAAAGACCTGTT+TGG | + | chr5.4:63308698-63308717 | MS.gene50125:intron | 20.0% |
| !! | GAAACTTATTATTAAGGTAT+AGG | - | chr5.4:63310206-63310225 | None:intergenic | 20.0% |
| !! | GATTATCAACTATCTTAAAT+GGG | - | chr5.4:63310694-63310713 | None:intergenic | 20.0% |
| !! | GTTAAGGTTAAAAAAGTTTA+TGG | - | chr5.4:63309250-63309269 | None:intergenic | 20.0% |
| !! | TAAATATGAATGACATACAA+AGG | - | chr5.4:63309105-63309124 | None:intergenic | 20.0% |
| !!! | AAAATGATTTTGGTTGATAT+AGG | + | chr5.4:63310646-63310665 | MS.gene50125:intron | 20.0% |
| !!! | AAATGATTTTGGTTGATATA+GGG | + | chr5.4:63310647-63310666 | MS.gene50125:intron | 20.0% |
| !!! | AACAATCTGAAATTTAGATT+GGG | + | chr5.4:63311237-63311256 | MS.gene50125:intron | 20.0% |
| !!! | AAGAATTGAAAGTATACAAA+CGG | - | chr5.4:63311362-63311381 | None:intergenic | 20.0% |
| !!! | TTAAAACTTTTAGCTTATCA+TGG | + | chr5.4:63308587-63308606 | MS.gene50125:intron | 20.0% |
| ! | AAAATTAACAGTTACCAAAC+AGG | - | chr5.4:63308715-63308734 | None:intergenic | 25.0% |
| ! | AAGAAGATAGGATATTTAAC+AGG | + | chr5.4:63310355-63310374 | MS.gene50125:intron | 25.0% |
| ! | AATCCTTAAAATTAGTCAAG+TGG | + | chr5.4:63309864-63309883 | MS.gene50125:intron | 25.0% |
| ! | ACAAAAGACTACAAAACAAA+AGG | - | chr5.4:63310453-63310472 | None:intergenic | 25.0% |
| ! | AGAAAATTTAGTGAGAAACT+GGG | + | chr5.4:63309722-63309741 | MS.gene50125:intron | 25.0% |
| ! | ATCAGACAATAACACTTAAA+AGG | + | chr5.4:63310720-63310739 | MS.gene50125:CDS | 25.0% |
| ! | ATCCTTAAAATTAGTCAAGT+GGG | + | chr5.4:63309865-63309884 | MS.gene50125:intron | 25.0% |
| ! | ATTTATATATAGGGTCTGTT+TGG | + | chr5.4:63308387-63308406 | MS.gene50125:intron | 25.0% |
| ! | CAACAAGATAATCATTGTTA+AGG | - | chr5.4:63309266-63309285 | None:intergenic | 25.0% |
| ! | CATTGATATCAAGTTTCTAT+TGG | + | chr5.4:63310230-63310249 | MS.gene50125:intron | 25.0% |
| ! | CTAATTCAAGTAGTTTATAG+CGG | + | chr5.4:63308784-63308803 | MS.gene50125:intron | 25.0% |
| ! | GGATTATCAACTATCTTAAA+TGG | - | chr5.4:63310695-63310714 | None:intergenic | 25.0% |
| ! | GGTAAAAATAAGTTATAAGC+TGG | - | chr5.4:63308523-63308542 | None:intergenic | 25.0% |
| ! | TAGAAAATTTAGTGAGAAAC+TGG | + | chr5.4:63309721-63309740 | MS.gene50125:intron | 25.0% |
| ! | TATCAAAAGATGCTATAACA+TGG | - | chr5.4:63308544-63308563 | None:intergenic | 25.0% |
| ! | TATTAAGGTATAGGAACAAA+AGG | - | chr5.4:63310197-63310216 | None:intergenic | 25.0% |
| ! | TTAAATATCCTATCTTCTTC+CGG | - | chr5.4:63310354-63310373 | None:intergenic | 25.0% |
| ! | TTTATGAAGAAAGAATTTGG+CGG | + | chr5.4:63309320-63309339 | MS.gene50125:intron | 25.0% |
| ! | TTTCTAATGTTTGTGTAGTT+TGG | + | chr5.4:63309759-63309778 | MS.gene50125:intron | 25.0% |
| !! | ACATCAGTGTTGTTAAATAA+AGG | + | chr5.4:63311084-63311103 | MS.gene50125:intron | 25.0% |
| !! | ATATGATTATCTTAGTTTGG+AGG | + | chr5.4:63309462-63309481 | MS.gene50125:intron | 25.0% |
| !! | CAATTTTACCCTGTAATATT+AGG | + | chr5.4:63308615-63308634 | MS.gene50125:intron | 25.0% |
| !! | GAACAATCTGAAATTTAGAT+TGG | + | chr5.4:63311236-63311255 | MS.gene50125:intron | 25.0% |
| !! | GTGATTGAATTTGTAGTTTT+TGG | - | chr5.4:63309153-63309172 | None:intergenic | 25.0% |
| !! | TTCAATTCTTGTTGTATCTA+AGG | + | chr5.4:63311372-63311391 | MS.gene50125:intron | 25.0% |
| !!! | AAAAAGTTTATGGCTTTGTT+TGG | - | chr5.4:63309240-63309259 | None:intergenic | 25.0% |
| !!! | AATTGTCTAATTTTTTCCAG+TGG | + | chr5.4:63309604-63309623 | MS.gene50125:intron | 25.0% |
| !!! | TAAGACATAAATTTGGTTTG+TGG | + | chr5.4:63308648-63308667 | MS.gene50125:intron | 25.0% |
| !!! | TTATTTCTATGAGCATATAG+CGG | + | chr5.4:63308750-63308769 | MS.gene50125:intron | 25.0% |
| !!! | TTTAAGATAGTTGATAATCC+AGG | + | chr5.4:63310695-63310714 | MS.gene50125:CDS | 25.0% |
| !!! | TTTTTGGTAAACTTAGCAAA+TGG | - | chr5.4:63309137-63309156 | None:intergenic | 25.0% |
| AAAGATTATCTTTCGTGAGA+GGG | + | chr5.4:63309509-63309528 | MS.gene50125:intron | 30.0% | |
| ACACTTAAAAGGACTTATCA+AGG | + | chr5.4:63310731-63310750 | MS.gene50125:CDS | 30.0% | |
| ATAGCTTATAGCTTATAAGC+TGG | + | chr5.4:63308674-63308693 | MS.gene50125:intron | 30.0% | |
| ATCTGTTCATCAGAAAAATC+AGG | - | chr5.4:63310971-63310990 | None:intergenic | 30.0% | |
| ATCTTAAATGGGAAATTGCT+TGG | - | chr5.4:63310683-63310702 | None:intergenic | 30.0% | |
| ATTTAACAGGTTGTACAATC+TGG | + | chr5.4:63310368-63310387 | MS.gene50125:intron | 30.0% | |
| CACAAGTACTTAGAAATAAG+GGG | + | chr5.4:63311460-63311479 | MS.gene50125:CDS | 30.0% | |
| CACGAAAGATAATCTTTAGT+TGG | - | chr5.4:63309506-63309525 | None:intergenic | 30.0% | |
| CCACAAGTACTTAGAAATAA+GGG | + | chr5.4:63311459-63311478 | MS.gene50125:CDS | 30.0% | |
| GTATTAATATGCTCAGAATC+AGG | - | chr5.4:63310523-63310542 | None:intergenic | 30.0% | |
| TAAAGATTATCTTTCGTGAG+AGG | + | chr5.4:63309508-63309527 | MS.gene50125:intron | 30.0% | |
| TCAACTTATAGCTTATAAGC+TGG | + | chr5.4:63308423-63308442 | MS.gene50125:intron | 30.0% | |
| TCCACAAGTACTTAGAAATA+AGG | + | chr5.4:63311458-63311477 | MS.gene50125:CDS | 30.0% | |
| TGATATCAAGTTTCTATTGG+TGG | + | chr5.4:63310233-63310252 | MS.gene50125:intron | 30.0% | |
| TGATCATCAAGATGATGATA+AGG | + | chr5.4:63310808-63310827 | MS.gene50125:CDS | 30.0% | |
| TTATGAAGAAAGAATTTGGC+GGG | + | chr5.4:63309321-63309340 | MS.gene50125:intron | 30.0% | |
| TTCGAAATATCCAAATTGGA+AGG | - | chr5.4:63309669-63309688 | None:intergenic | 30.0% | |
| TTCTCAAAAAGGATTCGAAT+GGG | + | chr5.4:63309805-63309824 | MS.gene50125:intron | 30.0% | |
| TTTCTCAAAAAGGATTCGAA+TGG | + | chr5.4:63309804-63309823 | MS.gene50125:intron | 30.0% | |
| TTTGTGTAGTTTGGAAACTT+AGG | + | chr5.4:63309768-63309787 | MS.gene50125:intron | 30.0% | |
| ! | AACCCACTTGACTAATTTTA+AGG | - | chr5.4:63309870-63309889 | None:intergenic | 30.0% |
| ! | AGAAGTACATACTGAAAATC+TGG | - | chr5.4:63311007-63311026 | None:intergenic | 30.0% |
| ! | AGATTCTTCCATGTTTTACA+GGG | + | chr5.4:63311408-63311427 | MS.gene50125:intron | 30.0% |
| ! | ATCACTTTCTGTTTGAAGTT+GGG | + | chr5.4:63309945-63309964 | MS.gene50125:intron | 30.0% |
| ! | GCTAGCTTATTTGTAATGTT+TGG | - | chr5.4:63308961-63308980 | None:intergenic | 30.0% |
| ! | GTAATGTTTGGTGAAATTAG+CGG | - | chr5.4:63308949-63308968 | None:intergenic | 30.0% |
| ! | GTTTTCAATTGAGATTGCAA+GGG | - | chr5.4:63308867-63308886 | None:intergenic | 30.0% |
| ! | TAACTGATAAACTAGCTTAG+AGG | - | chr5.4:63309202-63309221 | None:intergenic | 30.0% |
| ! | TGTTTTCAATTGAGATTGCA+AGG | - | chr5.4:63308868-63308887 | None:intergenic | 30.0% |
| ! | TTTTCAATTGAGATTGCAAG+GGG | - | chr5.4:63308866-63308885 | None:intergenic | 30.0% |
| !! | AATTTTCAGGGTTTTGTGTT+AGG | + | chr5.4:63309541-63309560 | MS.gene50125:intron | 30.0% |
| !! | GCATTCAATGTTTTAAACCA+GGG | + | chr5.4:63311059-63311078 | MS.gene50125:intron | 30.0% |
| !! | TAACATGATTGTTCTGAAAC+AGG | - | chr5.4:63308469-63308488 | None:intergenic | 30.0% |
| !! | TGGTTGGTAAGACATAAATT+TGG | + | chr5.4:63308641-63308660 | MS.gene50125:intron | 30.0% |
| !!! | GGTGTGATTTTTTCTCAAAA+AGG | + | chr5.4:63309794-63309813 | MS.gene50125:intron | 30.0% |
| !!! | TCTAATTTTTTCCAGTGGTT+TGG | + | chr5.4:63309609-63309628 | MS.gene50125:intron | 30.0% |
| !!! | TTTACCTCAAGAGTTTTTTC+CGG | + | chr5.4:63310320-63310339 | MS.gene50125:intron | 30.0% |
| AACCAGAGCCTAATATTACA+GGG | - | chr5.4:63308626-63308645 | None:intergenic | 35.0% | |
| AAGATTATCTTTCGTGAGAG+GGG | + | chr5.4:63309510-63309529 | MS.gene50125:intron | 35.0% | |
| AATCTACACCTCATTACCAT+CGG | - | chr5.4:63309400-63309419 | None:intergenic | 35.0% | |
| ACAGAAGAAAAGTGAAACGT+AGG | - | chr5.4:63308196-63308215 | None:intergenic | 35.0% | |
| AGAAACTGGGTGTGATTATT+TGG | + | chr5.4:63309735-63309754 | MS.gene50125:intron | 35.0% | |
| ATCCAAATCCCTGTAAAACA+TGG | - | chr5.4:63311419-63311438 | None:intergenic | 35.0% | |
| ATGAACAGATTCCTTATGAC+GGG | + | chr5.4:63310981-63311000 | MS.gene50125:CDS | 35.0% | |
| ATTGATCAGAAAAACCCAAC+CGG | + | chr5.4:63308117-63308136 | MS.gene50125:CDS | 35.0% | |
| CAAGGATAGTAGAGAATACT+TGG | + | chr5.4:63311528-63311547 | MS.gene50125:CDS | 35.0% | |
| CTTTCAAATAGCAGCTATAG+TGG | + | chr5.4:63311183-63311202 | MS.gene50125:intron | 35.0% | |
| GAAACGGAGAAGAATATCAT+CGG | - | chr5.4:63308242-63308261 | None:intergenic | 35.0% | |
| GATGAACAGATTCCTTATGA+CGG | + | chr5.4:63310980-63310999 | MS.gene50125:CDS | 35.0% | |
| GGAGTTCGAAATATCCAAAT+TGG | - | chr5.4:63309673-63309692 | None:intergenic | 35.0% | |
| GTAGTTTGGAAACTTAGGAA+CGG | + | chr5.4:63309773-63309792 | MS.gene50125:intron | 35.0% | |
| TGTCGATAACAATCTCATCT+GGG | - | chr5.4:63310931-63310950 | None:intergenic | 35.0% | |
| ! | AGTGTTATTGTCTGATTTCC+TGG | - | chr5.4:63310716-63310735 | None:intergenic | 35.0% |
| ! | AGTTCTATGTACTTGTTGAG+GGG | + | chr5.4:63309976-63309995 | MS.gene50125:intron | 35.0% |
| ! | AGTTGAGTTCATCTAGTTTC+AGG | + | chr5.4:63310140-63310159 | MS.gene50125:intron | 35.0% |
| ! | ATAGTAGAGAATACTTGGTG+TGG | + | chr5.4:63311533-63311552 | MS.gene50125:CDS | 35.0% |
| ! | ATGGTAATGAGGTGTAGATT+GGG | + | chr5.4:63309400-63309419 | MS.gene50125:intron | 35.0% |
| ! | CAGTTCTATGTACTTGTTGA+GGG | + | chr5.4:63309975-63309994 | MS.gene50125:intron | 35.0% |
| ! | GAGATTCTTCCATGTTTTAC+AGG | + | chr5.4:63311407-63311426 | MS.gene50125:intron | 35.0% |
| ! | GATCACTTTCTGTTTGAAGT+TGG | + | chr5.4:63309944-63309963 | MS.gene50125:intron | 35.0% |
| ! | GTTGAGTTCATCTAGTTTCA+GGG | + | chr5.4:63310141-63310160 | MS.gene50125:intron | 35.0% |
| ! | TCACTTTCTGTTTGAAGTTG+GGG | + | chr5.4:63309946-63309965 | MS.gene50125:intron | 35.0% |
| ! | TCAGCTATAAGCTAGCTTAT+AGG | + | chr5.4:63308991-63309010 | MS.gene50125:intron | 35.0% |
| ! | TCAGTGTTTCTAAGAAGGAT+GGG | + | chr5.4:63310876-63310895 | MS.gene50125:CDS | 35.0% |
| ! | TCAGTTCTATGTACTTGTTG+AGG | + | chr5.4:63309974-63309993 | MS.gene50125:intron | 35.0% |
| ! | TGATTTCCGATTCGATGAAA+CGG | - | chr5.4:63308258-63308277 | None:intergenic | 35.0% |
| ! | TTAGAAACACTGACTGAAAG+TGG | - | chr5.4:63310869-63310888 | None:intergenic | 35.0% |
| ! | TTCAGTCAGTGTTTCTAAGA+AGG | + | chr5.4:63310871-63310890 | MS.gene50125:CDS | 35.0% |
| ! | TTCCATGTTTTACAGGGATT+TGG | + | chr5.4:63311414-63311433 | MS.gene50125:intron | 35.0% |
| ! | TTCTTGTTGTATCTAAGGCA+TGG | + | chr5.4:63311377-63311396 | MS.gene50125:intron | 35.0% |
| !! | AGGTGAAGAAATTCAAGTTG+AGG | + | chr5.4:63310751-63310770 | MS.gene50125:CDS | 35.0% |
| !! | AGTGAGACTTTGAAATGTCT+TGG | + | chr5.4:63309632-63309651 | MS.gene50125:intron | 35.0% |
| !! | CCCTTATTTCTAAGTACTTG+TGG | - | chr5.4:63311462-63311481 | None:intergenic | 35.0% |
| !! | CGCATTCAATGTTTTAAACC+AGG | + | chr5.4:63311058-63311077 | MS.gene50125:intron | 35.0% |
| !! | CTATAGCACTTTTTGTGTAG+CGG | + | chr5.4:63311208-63311227 | MS.gene50125:intron | 35.0% |
| !! | GTGAGACTTTGAAATGTCTT+GGG | + | chr5.4:63309633-63309652 | MS.gene50125:intron | 35.0% |
| !! | TGAGACTTTGAAATGTCTTG+GGG | + | chr5.4:63309634-63309653 | MS.gene50125:intron | 35.0% |
| !! | TGCAAGAAATTTGTTGTGCT+TGG | - | chr5.4:63311493-63311512 | None:intergenic | 35.0% |
| !!! | AGGGGTTGGTATTAATTTTC+AGG | + | chr5.4:63309528-63309547 | MS.gene50125:intron | 35.0% |
| !!! | ATTTTGGTTGATATAGGGTG+AGG | + | chr5.4:63310652-63310671 | MS.gene50125:intron | 35.0% |
| !!! | GGGGTTGGTATTAATTTTCA+GGG | + | chr5.4:63309529-63309548 | MS.gene50125:intron | 35.0% |
| !!! | TTACCTCAAGAGTTTTTTCC+GGG | + | chr5.4:63310321-63310340 | MS.gene50125:intron | 35.0% |
| AACACAACTGAACTCCAAAG+AGG | - | chr5.4:63310903-63310922 | None:intergenic | 40.0% | |
| AAGTGATCTCTCAACAGAAC+AGG | - | chr5.4:63309932-63309951 | None:intergenic | 40.0% | |
| AATGAGGTGTAGATTGGGTA+GGG | + | chr5.4:63309405-63309424 | MS.gene50125:intron | 40.0% | |
| ACACAACTGAACTCCAAAGA+GGG | - | chr5.4:63310902-63310921 | None:intergenic | 40.0% | |
| ACAGGTTGTACAATCTGGTT+TGG | + | chr5.4:63310373-63310392 | MS.gene50125:intron | 40.0% | |
| AGAAGAATATCATCGGAGGT+GGG | - | chr5.4:63308235-63308254 | None:intergenic | 40.0% | |
| CAACCAGAGCCTAATATTAC+AGG | - | chr5.4:63308627-63308646 | None:intergenic | 40.0% | |
| CAAGTCAAGCACTTTCGTTA+GGG | + | chr5.4:63308075-63308094 | MS.gene50125:CDS | 40.0% | |
| CTGTCGATAACAATCTCATC+TGG | - | chr5.4:63310932-63310951 | None:intergenic | 40.0% | |
| GACTCTTTGACAGAGTTACT+CGG | - | chr5.4:63309360-63309379 | None:intergenic | 40.0% | |
| GCATGAGTACATGATCAACA+AGG | + | chr5.4:63311510-63311529 | MS.gene50125:CDS | 40.0% | |
| GCTTTCTCTTTCATCTTGTC+TGG | + | chr5.4:63310168-63310187 | MS.gene50125:intron | 40.0% | |
| GTAGAATTCCGGAAGAAGAT+AGG | + | chr5.4:63310343-63310362 | MS.gene50125:intron | 40.0% | |
| TAACAACACTGATGTAACCC+TGG | - | chr5.4:63311079-63311098 | None:intergenic | 40.0% | |
| TAATGAGGTGTAGATTGGGT+AGG | + | chr5.4:63309404-63309423 | MS.gene50125:intron | 40.0% | |
| TACCCTGTAATATTAGGCTC+TGG | + | chr5.4:63308621-63308640 | MS.gene50125:intron | 40.0% | |
| TCTTCACCAGTAACCAAATC+AGG | - | chr5.4:63310785-63310804 | None:intergenic | 40.0% | |
| TCTTCTCCGTTTCATCGAAT+CGG | + | chr5.4:63308249-63308268 | MS.gene50125:CDS | 40.0% | |
| TGATGTTGCAGTTCAGATTG+CGG | + | chr5.4:63311316-63311335 | MS.gene50125:intron | 40.0% | |
| TTGATCAGAAAAACCCAACC+GGG | + | chr5.4:63308118-63308137 | MS.gene50125:CDS | 40.0% | |
| ! | CAGGTTGTACAATCTGGTTT+GGG | + | chr5.4:63310374-63310393 | MS.gene50125:intron | 40.0% |
| ! | CTGTAATATTAGGCTCTGGT+TGG | + | chr5.4:63308625-63308644 | MS.gene50125:intron | 40.0% |
| ! | GATGGTAATGAGGTGTAGAT+TGG | + | chr5.4:63309399-63309418 | MS.gene50125:intron | 40.0% |
| ! | GCAAACTTTTGCTGCAAGTT+TGG | + | chr5.4:63310264-63310283 | MS.gene50125:intron | 40.0% |
| ! | GTCAGTGTTTCTAAGAAGGA+TGG | + | chr5.4:63310875-63310894 | MS.gene50125:CDS | 40.0% |
| ! | GTTCATCTAGTTTCAGGGTT+AGG | + | chr5.4:63310146-63310165 | MS.gene50125:intron | 40.0% |
| ! | GTTCTATGTACTTGTTGAGG+GGG | + | chr5.4:63309977-63309996 | MS.gene50125:intron | 40.0% |
| ! | GTTTTACAGGGATTTGGATG+AGG | + | chr5.4:63311420-63311439 | MS.gene50125:intron | 40.0% |
| ! | TTCTATGTACTTGTTGAGGG+GGG | + | chr5.4:63309978-63309997 | MS.gene50125:intron | 40.0% |
| !! | CTCTGTCAAAGAGTCGTTTT+TGG | + | chr5.4:63309365-63309384 | MS.gene50125:intron | 40.0% |
| !! | TTTCAGGGTTTTGTGTTAGG+AGG | + | chr5.4:63309544-63309563 | MS.gene50125:intron | 40.0% |
| !!! | AGTTTTTCAAGGGGTGTTTG+TGG | + | chr5.4:63310026-63310045 | MS.gene50125:intron | 40.0% |
| !!! | GTTTTTTCCGGGTAGAATTC+CGG | + | chr5.4:63310332-63310351 | MS.gene50125:intron | 40.0% |
| !!! | TGCACTCTGGAGTTTTTCAA+GGG | + | chr5.4:63310016-63310035 | MS.gene50125:intron | 40.0% |
| !!! | TTGCACTCTGGAGTTTTTCA+AGG | + | chr5.4:63310015-63310034 | MS.gene50125:intron | 40.0% |
| AAAGAATTTGGCGGGAGATG+GGG | + | chr5.4:63309329-63309348 | MS.gene50125:intron | 45.0% | |
| AAAGTCTCACTCCAAACCAC+TGG | - | chr5.4:63309623-63309642 | None:intergenic | 45.0% | |
| ACGGAGAAGAATATCATCGG+AGG | - | chr5.4:63308239-63308258 | None:intergenic | 45.0% | |
| ACGTAGGAACAAATGAACCC+TGG | - | chr5.4:63308180-63308199 | None:intergenic | 45.0% | |
| AGAAAGAATTTGGCGGGAGA+TGG | + | chr5.4:63309327-63309346 | MS.gene50125:intron | 45.0% | |
| ATCGGAAATCACTTGTGCTC+AGG | + | chr5.4:63308267-63308286 | MS.gene50125:CDS | 45.0% | |
| CCAAGTCAAGCACTTTCGTT+AGG | + | chr5.4:63308074-63308093 | MS.gene50125:CDS | 45.0% | |
| CTACCCGGAAAAAACTCTTG+AGG | - | chr5.4:63310327-63310346 | None:intergenic | 45.0% | |
| CTCTCAACAGAACAGGTCAA+GGG | - | chr5.4:63309925-63309944 | None:intergenic | 45.0% | |
| GAAAGAATTTGGCGGGAGAT+GGG | + | chr5.4:63309328-63309347 | MS.gene50125:intron | 45.0% | |
| GAGAAGAATATCATCGGAGG+TGG | - | chr5.4:63308236-63308255 | None:intergenic | 45.0% | |
| GCTCAGGAAACCGATGATTT+CGG | + | chr5.4:63308283-63308302 | MS.gene50125:CDS | 45.0% | |
| GTTAGGAGGATGTGGTACTT+TGG | + | chr5.4:63309558-63309577 | MS.gene50125:intron | 45.0% | |
| GTTTCGTTCGCCTTCCAATT+TGG | + | chr5.4:63309656-63309675 | MS.gene50125:intron | 45.0% | |
| TACTGCAGCAGGAGAAAGAA+AGG | - | chr5.4:63310091-63310110 | None:intergenic | 45.0% | |
| TATGCTCAGAATCAGGCACA+AGG | - | chr5.4:63310516-63310535 | None:intergenic | 45.0% | |
| TCTCTCAACAGAACAGGTCA+AGG | - | chr5.4:63309926-63309945 | None:intergenic | 45.0% | |
| TCTTCTTCCGGAATTCTACC+CGG | - | chr5.4:63310342-63310361 | None:intergenic | 45.0% | |
| TGAAAATCTGGCCCGTCATA+AGG | - | chr5.4:63310995-63311014 | None:intergenic | 45.0% | |
| TGAGGTTCACATGCCTGATT+TGG | + | chr5.4:63310769-63310788 | MS.gene50125:CDS | 45.0% | |
| TTATCTTTCGTGAGAGGGGT+TGG | + | chr5.4:63309514-63309533 | MS.gene50125:intron | 45.0% | |
| ! | ACTTACAGCGCCGAAATCAT+CGG | - | chr5.4:63308296-63308315 | None:intergenic | 45.0% |
| ! | ATTGGGTTGTGTTGCTAACC+TGG | + | chr5.4:63311254-63311273 | MS.gene50125:intron | 45.0% |
| ! | CAGCTAGTTAGTCTCAGGTT+TGG | + | chr5.4:63310293-63310312 | MS.gene50125:intron | 45.0% |
| ! | TGCAGTTCAGATTGCGGTTT+TGG | + | chr5.4:63311322-63311341 | MS.gene50125:intron | 45.0% |
| ! | TGTAGCAGCTAGTTAGTCTC+AGG | + | chr5.4:63310288-63310307 | MS.gene50125:intron | 45.0% |
| !! | CACATGCCTGATTTGGTTAC+TGG | + | chr5.4:63310776-63310795 | MS.gene50125:CDS | 45.0% |
| !! | CCTAACGAAAGTGCTTGACT+TGG | - | chr5.4:63308077-63308096 | None:intergenic | 45.0% |
| !! | GGTTTTGTGTTAGGAGGATG+TGG | + | chr5.4:63309550-63309569 | MS.gene50125:intron | 45.0% |
| !! | GTTGTACAATCTGGTTTGGG+TGG | + | chr5.4:63310377-63310396 | MS.gene50125:intron | 45.0% |
| !! | GTTTTGAGCTTGGCATGGTT+AGG | + | chr5.4:63310407-63310426 | MS.gene50125:intron | 45.0% |
| !! | TCAAGTTTCTATTGGTGGCG+TGG | + | chr5.4:63310238-63310257 | MS.gene50125:intron | 45.0% |
| !! | TGAACCCTGGTATCGATTGA+CGG | - | chr5.4:63308167-63308186 | None:intergenic | 45.0% |
| !!! | GCACTCTGGAGTTTTTCAAG+GGG | + | chr5.4:63310017-63310036 | MS.gene50125:intron | 45.0% |
| !!! | TGGTGTGTCAGTTTTGAGCT+TGG | + | chr5.4:63310397-63310416 | MS.gene50125:intron | 45.0% |
| !!! | TGTCAGTTTTGAGCTTGGCA+TGG | + | chr5.4:63310402-63310421 | MS.gene50125:intron | 45.0% |
| !!! | TTTTTGGTCTTGAGCACCGA+TGG | + | chr5.4:63309381-63309400 | MS.gene50125:intron | 45.0% |
| !! | AAAAAAAAAAAAAATTGAAA+AGG | - | chr5.4:63310552-63310571 | None:intergenic | 5.0% |
| !!! | TTTAATTTCATTTATATATA+GGG | + | chr5.4:63308378-63308397 | MS.gene50125:intron | 5.0% |
| !!! | TTTTAATTTCATTTATATAT+AGG | + | chr5.4:63308377-63308396 | MS.gene50125:intron | 5.0% |
| AAGAATTTGGCGGGAGATGG+GGG | + | chr5.4:63309330-63309349 | MS.gene50125:intron | 50.0% | |
| GGGTAGGGCTGTGGATTTAT+GGG | + | chr5.4:63309420-63309439 | MS.gene50125:intron | 50.0% | |
| TAAGAAGGATGGGCCCTCTT+TGG | + | chr5.4:63310886-63310905 | MS.gene50125:CDS | 50.0% | |
| TCTCTACAACCAAGCAGTCC+AGG | - | chr5.4:63311275-63311294 | None:intergenic | 50.0% | |
| TGGGTAGGGCTGTGGATTTA+TGG | + | chr5.4:63309419-63309438 | MS.gene50125:intron | 50.0% | |
| TGTTGCTAACCTGGACTGCT+TGG | + | chr5.4:63311263-63311282 | MS.gene50125:intron | 50.0% | |
| TTTCTCCTGCTGCAGTAGTG+AGG | + | chr5.4:63310094-63310113 | MS.gene50125:intron | 50.0% | |
| ! | TATGTACTTGTTGAGGGGGG+TGG | + | chr5.4:63309981-63310000 | MS.gene50125:intron | 50.0% |
| !! | CTTGAGCACCGATGGTAATG+AGG | + | chr5.4:63309389-63309408 | MS.gene50125:intron | 50.0% |
| !! | GTGTTGCTTCAGTTGCACTC+TGG | + | chr5.4:63310003-63310022 | MS.gene50125:intron | 50.0% |
| GAGGTCTGAGTCTGACTGCT+TGG | + | chr5.4:63310113-63310132 | MS.gene50125:intron | 55.0% | |
| GTGTAGATTGGGTAGGGCTG+TGG | + | chr5.4:63309411-63309430 | MS.gene50125:intron | 55.0% | |
| TCAGACCTCACTACTGCAGC+AGG | - | chr5.4:63310102-63310121 | None:intergenic | 55.0% | |
| !! | ACCCTGGTATCGATTGACGG+CGG | - | chr5.4:63308164-63308183 | None:intergenic | 55.0% |
| !! | GTTTCTATTGGTGGCGTGGC+AGG | + | chr5.4:63310242-63310261 | MS.gene50125:intron | 55.0% |
| ATTGACGGCGGCGGAAAGCA+CGG | - | chr5.4:63308152-63308171 | None:intergenic | 60.0% | |
| GCCGCCGTCAATCGATACCA+GGG | + | chr5.4:63308160-63308179 | MS.gene50125:CDS | 60.0% | |
| !! | CTGGTATCGATTGACGGCGG+CGG | - | chr5.4:63308161-63308180 | None:intergenic | 60.0% |
| CGCCGCCGTCAATCGATACC+AGG | + | chr5.4:63308159-63308178 | MS.gene50125:CDS | 65.0% | |
| ! | GAAAGCACGGCGCGATGACC+CGG | - | chr5.4:63308139-63308158 | None:intergenic | 65.0% |
| CACGGCGCGATGACCCGGTT+GGG | - | chr5.4:63308134-63308153 | None:intergenic | 70.0% | |
| GCACGGCGCGATGACCCGGT+TGG | - | chr5.4:63308135-63308154 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 63308049 | 63311588 | 63308049 | ID=MS.gene50125 |
| chr5.4 | mRNA | 63308049 | 63311588 | 63308049 | ID=MS.gene50125.t1;Parent=MS.gene50125 |
| chr5.4 | exon | 63308049 | 63308309 | 63308049 | ID=MS.gene50125.t1.exon1;Parent=MS.gene50125.t1 |
| chr5.4 | CDS | 63308049 | 63308309 | 63308049 | ID=cds.MS.gene50125.t1;Parent=MS.gene50125.t1 |
| chr5.4 | exon | 63310668 | 63311014 | 63310668 | ID=MS.gene50125.t1.exon2;Parent=MS.gene50125.t1 |
| chr5.4 | CDS | 63310668 | 63311014 | 63310668 | ID=cds.MS.gene50125.t1;Parent=MS.gene50125.t1 |
| chr5.4 | exon | 63311429 | 63311588 | 63311429 | ID=MS.gene50125.t1.exon3;Parent=MS.gene50125.t1 |
| chr5.4 | CDS | 63311429 | 63311588 | 63311429 | ID=cds.MS.gene50125.t1;Parent=MS.gene50125.t1 |
| Gene Sequence |
| Protein sequence |
