Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50127.t1 | XP_024641057.1 | 79.9 | 259 | 45 | 4 | 1 | 256 | 1 | 255 | 4.20E-92 | 347.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50127.t1 | Q8W487 | 48.5 | 194 | 87 | 4 | 68 | 255 | 63 | 249 | 3.2e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50127.t1 | A0A396I021 | 79.9 | 259 | 45 | 4 | 1 | 256 | 1 | 255 | 3.0e-92 | 347.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene46104 | MS.gene50127 | PPI |
| MS.gene46105 | MS.gene50127 | PPI |
| MS.gene89319 | MS.gene50127 | PPI |
| MS.gene053230 | MS.gene50127 | PPI |
| MS.gene040271 | MS.gene50127 | PPI |
| MS.gene055201 | MS.gene50127 | PPI |
| MS.gene67544 | MS.gene50127 | PPI |
| MS.gene040272 | MS.gene50127 | PPI |
| MS.gene040269 | MS.gene50127 | PPI |
| MS.gene034255 | MS.gene50127 | PPI |
| MS.gene000604 | MS.gene50127 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50127.t1 | MTR_5g080880 | 80.620 | 258 | 43 | 4 | 1 | 255 | 1 | 254 | 6.31e-133 | 379 |
| MS.gene50127.t1 | MTR_5g080870 | 70.312 | 256 | 40 | 5 | 1 | 256 | 1 | 220 | 5.21e-116 | 336 |
| MS.gene50127.t1 | MTR_7g063520 | 42.292 | 253 | 122 | 6 | 8 | 255 | 20 | 253 | 2.18e-54 | 176 |
| MS.gene50127.t1 | MTR_4g006560 | 35.849 | 159 | 94 | 4 | 99 | 255 | 83 | 235 | 1.46e-21 | 90.5 |
| MS.gene50127.t1 | MTR_1g013120 | 30.864 | 243 | 143 | 10 | 24 | 255 | 4 | 232 | 1.17e-19 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50127.t1 | AT3G55605 | 41.758 | 273 | 125 | 10 | 1 | 255 | 1 | 257 | 8.19e-50 | 164 |
| MS.gene50127.t1 | AT5G05990 | 37.218 | 266 | 142 | 6 | 5 | 255 | 3 | 258 | 1.09e-48 | 161 |
| MS.gene50127.t1 | AT2G39795 | 43.299 | 194 | 97 | 4 | 68 | 255 | 63 | 249 | 1.08e-44 | 151 |
| MS.gene50127.t1 | AT5G02050 | 44.500 | 200 | 95 | 5 | 68 | 255 | 71 | 266 | 6.07e-42 | 144 |
| MS.gene50127.t1 | AT2G39790 | 31.559 | 263 | 150 | 9 | 1 | 256 | 1 | 240 | 3.75e-26 | 102 |
| MS.gene50127.t1 | AT1G15870 | 32.919 | 161 | 97 | 5 | 98 | 255 | 88 | 240 | 7.05e-19 | 83.2 |
| MS.gene50127.t1 | AT1G80720 | 30.723 | 166 | 103 | 5 | 94 | 255 | 31 | 188 | 2.63e-15 | 72.4 |
| MS.gene50127.t1 | AT1G80720 | 30.723 | 166 | 103 | 5 | 94 | 255 | 83 | 240 | 3.72e-15 | 73.2 |
| MS.gene50127.t1 | AT4G31930 | 30.247 | 162 | 98 | 5 | 99 | 254 | 80 | 232 | 8.95e-11 | 60.8 |
Find 40 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCATGAACTATCTTAAA+TGG | 0.152828 | 5.4:-63301233 | None:intergenic |
| TGTTCATCATAACACTTAAA+AGG | 0.286226 | 5.4:+63301261 | MS.gene50127:CDS |
| TAAGAAGAATGGGCTCTCTT+TGG | 0.316384 | 5.4:+63301430 | MS.gene50127:CDS |
| TTATCAAGGTGAAGAAATTC+AGG | 0.325526 | 5.4:+63301286 | MS.gene50127:CDS |
| TGCAAGAAATTTGTTGTGCT+TGG | 0.355860 | 5.4:-63301930 | None:intergenic |
| CTGCGGATAACCATCTCATC+TGG | 0.366391 | 5.4:-63301473 | None:intergenic |
| AGTGTTATGATGAACAGATT+AGG | 0.395452 | 5.4:-63301254 | None:intergenic |
| GTCAGTGTTTCTAAGAAGAA+TGG | 0.401567 | 5.4:+63301419 | MS.gene50127:CDS |
| CTCTTATTTCTAAGTACTTG+TGG | 0.408675 | 5.4:-63301899 | None:intergenic |
| TGCAAGAGTACATGATCAAC+TGG | 0.419125 | 5.4:+63301949 | MS.gene50127:CDS |
| CTGGAAAAGTAGAGAATACT+TGG | 0.432278 | 5.4:+63301968 | MS.gene50127:CDS |
| CACAAGTACTTAGAAATAAG+AGG | 0.434165 | 5.4:+63301900 | MS.gene50127:CDS |
| AACAAATTCGTTATTATGAC+AGG | 0.438496 | 5.4:+63301519 | MS.gene50127:CDS |
| GTCTCACTCTCATCGGAGTT+GGG | 0.439253 | 5.4:-63300898 | None:intergenic |
| CGTCGCCGTCAACCGCTACA+AGG | 0.457906 | 5.4:+63300825 | MS.gene50127:CDS |
| TTAGAAACACTGACTGAAAG+TGG | 0.463235 | 5.4:-63301410 | None:intergenic |
| GATCATGAACTATCTTAAAT+GGG | 0.467389 | 5.4:-63301232 | None:intergenic |
| TAAGCATCAAAAGGATGATA+AGG | 0.471842 | 5.4:+63301352 | MS.gene50127:CDS |
| ACAGAAGAAAAGTGAAACTT+AGG | 0.474140 | 5.4:-63300859 | None:intergenic |
| AGTCTCACTCTCATCGGAGT+TGG | 0.474313 | 5.4:-63300899 | None:intergenic |
| ATCGAAAATCACTTGTGCTC+AGG | 0.490320 | 5.4:+63300939 | MS.gene50127:CDS |
| TGAACCCTTGTAGCGGTTGA+CGG | 0.505939 | 5.4:-63300830 | None:intergenic |
| TCAGTGTTTCTAAGAAGAAT+GGG | 0.508747 | 5.4:+63301420 | MS.gene50127:CDS |
| AGAACATGATAAGCATCAAA+AGG | 0.514345 | 5.4:+63301343 | MS.gene50127:CDS |
| ATCTTAAATGGGAAATTGCT+CGG | 0.515748 | 5.4:-63301221 | None:intergenic |
| TGCTTATCATGTTCTTCACC+AGG | 0.518527 | 5.4:-63301335 | None:intergenic |
| ACACTTAAAAGGACTTATCA+AGG | 0.527097 | 5.4:+63301272 | MS.gene50127:CDS |
| GTTTACAGCGCGGAACTCAT+CGG | 0.541419 | 5.4:-63300965 | None:intergenic |
| AGGTGAAGAAATTCAGGTTG+AGG | 0.552447 | 5.4:+63301292 | MS.gene50127:CDS |
| GAAAGCACGGCACGATGACC+CGG | 0.553123 | 5.4:-63300802 | None:intergenic |
| GAGAAGAGTCTCACTCTCAT+CGG | 0.570988 | 5.4:-63300905 | None:intergenic |
| CTTGTAGCGGTTGACGGCGA+CGG | 0.585839 | 5.4:-63300824 | None:intergenic |
| TGCGGATAACCATCTCATCT+GGG | 0.591316 | 5.4:-63301472 | None:intergenic |
| GTTGACGGCGACGGAAAGCA+CGG | 0.602741 | 5.4:-63300815 | None:intergenic |
| TGTTGCTTACCCAGATGAGA+TGG | 0.609066 | 5.4:+63301463 | MS.gene50127:CDS |
| GTCGCCGTCAACCGCTACAA+GGG | 0.618745 | 5.4:+63300826 | MS.gene50127:CDS |
| TCTAACGAAAGTGCTTGACT+TGG | 0.627309 | 5.4:-63300740 | None:intergenic |
| GATTCTTAATAAACAAGCTG+CGG | 0.629799 | 5.4:-63301490 | None:intergenic |
| AAAGTAGAGAATACTTGGTG+TGG | 0.686000 | 5.4:+63301973 | MS.gene50127:CDS |
| GAACAAATGAACCCTTGTAG+CGG | 0.686097 | 5.4:-63300837 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATGTTGAAAATTTGATTT+TGG | + | chr5.4:63301177-63301196 | MS.gene50127:intron | 15.0% |
| !! | AATTGAAAGTAATACAAACA+GGG | - | chr5.4:63301821-63301840 | None:intergenic | 20.0% |
| !! | ATGGTATGAAAATTTGAATT+TGG | + | chr5.4:63301122-63301141 | MS.gene50127:intron | 20.0% |
| !! | TAATTGAAAGTAATACAAAC+AGG | - | chr5.4:63301822-63301841 | None:intergenic | 20.0% |
| !!! | AATTTGATTTTGGTTGATAT+AGG | + | chr5.4:63301187-63301206 | MS.gene50127:intron | 20.0% |
| !!! | ATTTGATTTTGGTTGATATA+GGG | + | chr5.4:63301188-63301207 | MS.gene50127:intron | 20.0% |
| !!! | TGATTTTCGATTCAATTAAA+TGG | - | chr5.4:63300930-63300949 | None:intergenic | 20.0% |
| ! | AACAAATTCGTTATTATGAC+AGG | + | chr5.4:63301519-63301538 | MS.gene50127:CDS | 25.0% |
| ! | GATCATGAACTATCTTAAAT+GGG | - | chr5.4:63301235-63301254 | None:intergenic | 25.0% |
| ! | TGTTCATCATAACACTTAAA+AGG | + | chr5.4:63301261-63301280 | MS.gene50127:CDS | 25.0% |
| !! | ATTTTCATACCATTACGAAA+TGG | - | chr5.4:63301115-63301134 | None:intergenic | 25.0% |
| !! | TTTTCATACCATTACGAAAT+GGG | - | chr5.4:63301114-63301133 | None:intergenic | 25.0% |
| !!! | TAGCTAATACATTTTTTCCA+GGG | + | chr5.4:63301848-63301867 | MS.gene50127:intron | 25.0% |
| AAAAAAGACCCATTTCGTAA+TGG | + | chr5.4:63301103-63301122 | MS.gene50127:intron | 30.0% | |
| ACACTTAAAAGGACTTATCA+AGG | + | chr5.4:63301272-63301291 | MS.gene50127:CDS | 30.0% | |
| ACAGAAGAAAAGTGAAACTT+AGG | - | chr5.4:63300862-63300881 | None:intergenic | 30.0% | |
| AGAACATGATAAGCATCAAA+AGG | + | chr5.4:63301343-63301362 | MS.gene50127:CDS | 30.0% | |
| AGTGTTATGATGAACAGATT+AGG | - | chr5.4:63301257-63301276 | None:intergenic | 30.0% | |
| ATCTTAAATGGGAAATTGCT+CGG | - | chr5.4:63301224-63301243 | None:intergenic | 30.0% | |
| CACAAGTACTTAGAAATAAG+AGG | + | chr5.4:63301900-63301919 | MS.gene50127:CDS | 30.0% | |
| GATTCTTAATAAACAAGCTG+CGG | - | chr5.4:63301493-63301512 | None:intergenic | 30.0% | |
| GGATCATGAACTATCTTAAA+TGG | - | chr5.4:63301236-63301255 | None:intergenic | 30.0% | |
| TAAGCATCAAAAGGATGATA+AGG | + | chr5.4:63301352-63301371 | MS.gene50127:CDS | 30.0% | |
| TGAACTTATAGCTTATAAGC+TGG | + | chr5.4:63301073-63301092 | MS.gene50127:intron | 30.0% | |
| TTATCAAGGTGAAGAAATTC+AGG | + | chr5.4:63301286-63301305 | MS.gene50127:CDS | 30.0% | |
| ! | AACTACTATTTTCCGTGATT+TGG | + | chr5.4:63301691-63301710 | MS.gene50127:intron | 30.0% |
| ! | TCAGTGTTTCTAAGAAGAAT+GGG | + | chr5.4:63301420-63301439 | MS.gene50127:CDS | 30.0% |
| !! | CTCTTATTTCTAAGTACTTG+TGG | - | chr5.4:63301902-63301921 | None:intergenic | 30.0% |
| !!! | ATACATTTTTTCCAGGGATT+TGG | + | chr5.4:63301854-63301873 | MS.gene50127:intron | 30.0% |
| !!! | GTAGCTAATACATTTTTTCC+AGG | + | chr5.4:63301847-63301866 | MS.gene50127:intron | 30.0% |
| CCAATCTCAATTCCAAATCA+CGG | - | chr5.4:63301706-63301725 | None:intergenic | 35.0% | |
| CTGGAAAAGTAGAGAATACT+TGG | + | chr5.4:63301968-63301987 | MS.gene50127:CDS | 35.0% | |
| GTGCATTCAATATGTGAAAC+CGG | + | chr5.4:63301595-63301614 | MS.gene50127:intron | 35.0% | |
| GTTGATCAGAAAAACTCAAC+CGG | + | chr5.4:63300783-63300802 | MS.gene50127:CDS | 35.0% | |
| TGCATTCAATATGTGAAACC+GGG | + | chr5.4:63301596-63301615 | MS.gene50127:intron | 35.0% | |
| TTGATCAGAAAAACTCAACC+GGG | + | chr5.4:63300784-63300803 | MS.gene50127:CDS | 35.0% | |
| ! | AAAGTAGAGAATACTTGGTG+TGG | + | chr5.4:63301973-63301992 | MS.gene50127:CDS | 35.0% |
| ! | CGTGATTTGGAATTGAGATT+GGG | + | chr5.4:63301704-63301723 | MS.gene50127:intron | 35.0% |
| ! | GTCAGTGTTTCTAAGAAGAA+TGG | + | chr5.4:63301419-63301438 | MS.gene50127:CDS | 35.0% |
| ! | TTAGAAACACTGACTGAAAG+TGG | - | chr5.4:63301413-63301432 | None:intergenic | 35.0% |
| !! | TGCAAGAAATTTGTTGTGCT+TGG | - | chr5.4:63301933-63301952 | None:intergenic | 35.0% |
| !!! | ATTTTGGTTGATATAGGGTG+AGG | + | chr5.4:63301193-63301212 | MS.gene50127:intron | 35.0% |
| !!! | TGCAGTTCAGATTGTTGTTT+TGG | + | chr5.4:63301780-63301799 | MS.gene50127:intron | 35.0% |
| ATCGAAAATCACTTGTGCTC+AGG | + | chr5.4:63300939-63300958 | MS.gene50127:CDS | 40.0% | |
| CCACTATAGCTGCTATGATA+TGG | - | chr5.4:63301635-63301654 | None:intergenic | 40.0% | |
| CCATATCATAGCAGCTATAG+TGG | + | chr5.4:63301632-63301651 | MS.gene50127:intron | 40.0% | |
| GAACAAATGAACCCTTGTAG+CGG | - | chr5.4:63300840-63300859 | None:intergenic | 40.0% | |
| GCATTCAATATGTGAAACCG+GGG | + | chr5.4:63301597-63301616 | MS.gene50127:intron | 40.0% | |
| TGCTTATCATGTTCTTCACC+AGG | - | chr5.4:63301338-63301357 | None:intergenic | 40.0% | |
| ! | CCGTGATTTGGAATTGAGAT+TGG | + | chr5.4:63301703-63301722 | MS.gene50127:intron | 40.0% |
| ! | GATTTGGAATTGAGATTGGG+TGG | + | chr5.4:63301707-63301726 | MS.gene50127:intron | 40.0% |
| ! | GCTATAGCACTTTTGCATAG+AGG | + | chr5.4:63301658-63301677 | MS.gene50127:intron | 40.0% |
| ! | TAAGAAGAATGGGCTCTCTT+TGG | + | chr5.4:63301430-63301449 | MS.gene50127:CDS | 40.0% |
| ! | TGCAAGAGTACATGATCAAC+TGG | + | chr5.4:63301949-63301968 | MS.gene50127:CDS | 40.0% |
| ! | TTTTTCCAGGGATTTGGATG+AGG | + | chr5.4:63301860-63301879 | MS.gene50127:intron | 40.0% |
| !! | AGGTGAAGAAATTCAGGTTG+AGG | + | chr5.4:63301292-63301311 | MS.gene50127:CDS | 40.0% |
| !! | TCTAACGAAAGTGCTTGACT+TGG | - | chr5.4:63300743-63300762 | None:intergenic | 40.0% |
| !! | TGAGGCTGCGATTTTTGATT+TGG | + | chr5.4:63301310-63301329 | MS.gene50127:CDS | 40.0% |
| !!! | GCGATTTTTGATTTGGTTCC+TGG | + | chr5.4:63301317-63301336 | MS.gene50127:CDS | 40.0% |
| AGAAGACGAAGTTTACAGCG+CGG | - | chr5.4:63300978-63300997 | None:intergenic | 45.0% | |
| GAGAAGAGTCTCACTCTCAT+CGG | - | chr5.4:63300908-63300927 | None:intergenic | 45.0% | |
| TATGGTCACTGATGTAACCC+CGG | - | chr5.4:63301617-63301636 | None:intergenic | 45.0% | |
| TGCGGATAACCATCTCATCT+GGG | - | chr5.4:63301475-63301494 | None:intergenic | 45.0% | |
| ! | TGTTGCTTACCCAGATGAGA+TGG | + | chr5.4:63301463-63301482 | MS.gene50127:CDS | 45.0% |
| AGTCTCACTCTCATCGGAGT+TGG | - | chr5.4:63300902-63300921 | None:intergenic | 50.0% | |
| CTGCGGATAACCATCTCATC+TGG | - | chr5.4:63301476-63301495 | None:intergenic | 50.0% | |
| GTCTCACTCTCATCGGAGTT+GGG | - | chr5.4:63300901-63300920 | None:intergenic | 50.0% | |
| GTTTACAGCGCGGAACTCAT+CGG | - | chr5.4:63300968-63300987 | None:intergenic | 50.0% | |
| TCTCTACAACCAAGCAGTGC+AGG | - | chr5.4:63301742-63301761 | None:intergenic | 50.0% | |
| TGAACCCTTGTAGCGGTTGA+CGG | - | chr5.4:63300833-63300852 | None:intergenic | 50.0% | |
| TGTTGCTAACCTGCACTGCT+TGG | + | chr5.4:63301730-63301749 | MS.gene50127:intron | 50.0% | |
| CAACGCCTCATCCAAATCCC+TGG | - | chr5.4:63301868-63301887 | None:intergenic | 55.0% | |
| CTTGTAGCGGTTGACGGCGA+CGG | - | chr5.4:63300827-63300846 | None:intergenic | 60.0% | |
| GTCGCCGTCAACCGCTACAA+GGG | + | chr5.4:63300826-63300845 | MS.gene50127:CDS | 60.0% | |
| GTTGACGGCGACGGAAAGCA+CGG | - | chr5.4:63300818-63300837 | None:intergenic | 60.0% | |
| ! | GAAAGCACGGCACGATGACC+CGG | - | chr5.4:63300805-63300824 | None:intergenic | 60.0% |
| CGTCGCCGTCAACCGCTACA+AGG | + | chr5.4:63300825-63300844 | MS.gene50127:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 63300715 | 63302028 | 63300715 | ID=MS.gene50127 |
| chr5.4 | mRNA | 63300715 | 63302028 | 63300715 | ID=MS.gene50127.t1;Parent=MS.gene50127 |
| chr5.4 | exon | 63300715 | 63300981 | 63300715 | ID=MS.gene50127.t1.exon1;Parent=MS.gene50127.t1 |
| chr5.4 | CDS | 63300715 | 63300981 | 63300715 | ID=cds.MS.gene50127.t1;Parent=MS.gene50127.t1 |
| chr5.4 | exon | 63301209 | 63301552 | 63301209 | ID=MS.gene50127.t1.exon2;Parent=MS.gene50127.t1 |
| chr5.4 | CDS | 63301209 | 63301552 | 63301209 | ID=cds.MS.gene50127.t1;Parent=MS.gene50127.t1 |
| chr5.4 | exon | 63301869 | 63302028 | 63301869 | ID=MS.gene50127.t1.exon3;Parent=MS.gene50127.t1 |
| chr5.4 | CDS | 63301869 | 63302028 | 63301869 | ID=cds.MS.gene50127.t1;Parent=MS.gene50127.t1 |
| Gene Sequence |
| Protein sequence |
