Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50848.t1 | XP_003598033.2 | 97.3 | 480 | 12 | 1 | 1 | 480 | 50 | 528 | 3.40E-252 | 880.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50848.t1 | Q9FVW4 | 47.5 | 488 | 189 | 12 | 1 | 483 | 48 | 473 | 3.1e-92 | 340.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50848.t1 | G7IYF2 | 97.3 | 480 | 12 | 1 | 1 | 480 | 50 | 528 | 2.5e-252 | 880.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene50848 | 0.801008 | 1.10E-48 | -1.69E-46 |
| MS.gene049499 | MS.gene50848 | 0.806074 | 9.81E-50 | -1.69E-46 |
| MS.gene051209 | MS.gene50848 | 0.809807 | 1.58E-50 | -1.69E-46 |
| MS.gene051332 | MS.gene50848 | 0.804095 | 2.55E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene50848 | 0.80375 | 3.00E-49 | -1.69E-46 |
| MS.gene052775 | MS.gene50848 | 0.81452 | 1.48E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene50848 | 0.813124 | 3.00E-51 | -1.69E-46 |
| MS.gene056050 | MS.gene50848 | 0.803942 | 2.74E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene50848 | 0.821982 | 3.02E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene50848 | 0.822915 | 1.83E-53 | -1.69E-46 |
| MS.gene057598 | MS.gene50848 | 0.810581 | 1.07E-50 | -1.69E-46 |
| MS.gene057855 | MS.gene50848 | 0.830578 | 2.71E-55 | -1.69E-46 |
| MS.gene058292 | MS.gene50848 | 0.801187 | 1.01E-48 | -1.69E-46 |
| MS.gene058427 | MS.gene50848 | 0.815685 | 8.15E-52 | -1.69E-46 |
| MS.gene058781 | MS.gene50848 | 0.801622 | 8.26E-49 | -1.69E-46 |
| MS.gene058919 | MS.gene50848 | 0.805379 | 1.37E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene50848 | 0.820844 | 5.53E-53 | -1.69E-46 |
| MS.gene060933 | MS.gene50848 | 0.828147 | 1.06E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene50848 | 0.834199 | 3.44E-56 | -1.69E-46 |
| MS.gene060937 | MS.gene50848 | 0.840175 | 1.02E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 113 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGCTTTGAAATGAGTTTA+AGG | 0.166330 | 3.4:+1089774 | None:intergenic |
| AGATCCCCGTCTGGTATTAC+TGG | 0.217254 | 3.4:+1088200 | None:intergenic |
| GAGTTTGCGGAGTTCTTTAA+TGG | 0.236801 | 3.4:-1090664 | MS.gene50848:CDS |
| ATTCTGAAGGGGAATCTAAA+AGG | 0.281820 | 3.4:-1083706 | MS.gene50848:CDS |
| TCTTCTGAATCAAGTATAAA+AGG | 0.294483 | 3.4:-1086414 | MS.gene50848:CDS |
| GGTAAAAGAAAATGCCTATT+CGG | 0.304563 | 3.4:-1083345 | MS.gene50848:CDS |
| CTTGCCTTGCATAGCAGCTC+TGG | 0.315043 | 3.4:+1088230 | None:intergenic |
| AGGCGTCCACCTCGTCCTTT+TGG | 0.319628 | 3.4:-1083782 | MS.gene50848:CDS |
| GTCATACCTTCAACTGATTC+AGG | 0.322351 | 3.4:+1083497 | None:intergenic |
| TTCCACCGGCTTCACGCCTT+TGG | 0.335343 | 3.4:+1083903 | None:intergenic |
| GCTGCTATGCAAGGCAAGCC+TGG | 0.337012 | 3.4:-1088225 | MS.gene50848:CDS |
| CAGGCCTGATCGTGGTGTTT+GGG | 0.338312 | 3.4:-1083600 | MS.gene50848:CDS |
| CCATCATCCTTCACTATATA+GGG | 0.349986 | 3.4:+1083107 | None:intergenic |
| ACAGGCCTGATCGTGGTGTT+TGG | 0.351241 | 3.4:-1083601 | MS.gene50848:CDS |
| ACCACTCCTGTTTCCCGAAT+AGG | 0.372521 | 3.4:+1083331 | None:intergenic |
| GGCATTGAAGGTTCCGTCTC+TGG | 0.374822 | 3.4:-1084406 | MS.gene50848:CDS |
| ACCATCATCCTTCACTATAT+AGG | 0.376532 | 3.4:+1083106 | None:intergenic |
| AGGGGAATCTAAAAGGGATT+TGG | 0.379049 | 3.4:-1083699 | MS.gene50848:CDS |
| ACGGGGATCTGAAAAGAAAA+AGG | 0.392701 | 3.4:-1088187 | MS.gene50848:intron |
| AGAAGAATGGTCCTCACGCC+TGG | 0.398858 | 3.4:+1086431 | None:intergenic |
| CGTGAAGGGACTATATATAA+AGG | 0.410366 | 3.4:-1089933 | MS.gene50848:intron |
| AAAATGCCTATTCGGGAAAC+AGG | 0.412330 | 3.4:-1083337 | MS.gene50848:CDS |
| GTAAAAGAAAATGCCTATTC+GGG | 0.413952 | 3.4:-1083344 | MS.gene50848:CDS |
| TTAAGGAACTCGAGATAGTC+TGG | 0.424224 | 3.4:+1089791 | None:intergenic |
| TCCAAAGCCGAGTACCAAAA+AGG | 0.427328 | 3.4:-1089961 | MS.gene50848:CDS |
| TCCGAGTGCAGAAATACAAT+TGG | 0.428949 | 3.4:-1089741 | MS.gene50848:CDS |
| CAAATCTTAACTTCTGAGAA+TGG | 0.431636 | 3.4:-1083806 | MS.gene50848:CDS |
| AAACTTTCAAAGAAAGGTGT+TGG | 0.453145 | 3.4:-1083062 | MS.gene50848:CDS |
| CTTTCAGGCCACTGAAGGTA+TGG | 0.458031 | 3.4:-1083984 | MS.gene50848:intron |
| GATCCCCGTCTGGTATTACT+GGG | 0.459889 | 3.4:+1088201 | None:intergenic |
| CATCTTGTGTCAGGTTTCAT+CGG | 0.460552 | 3.4:-1088277 | MS.gene50848:intron |
| TATACAGTGCATGGCATTGA+AGG | 0.462061 | 3.4:-1084418 | MS.gene50848:CDS |
| AACTTTCAAAGAAAGGTGTT+GGG | 0.465614 | 3.4:-1083061 | MS.gene50848:CDS |
| TTATACTTGATTCAGAAGAA+TGG | 0.467658 | 3.4:+1086418 | None:intergenic |
| ATTACTAGAATGTGAAGAAT+CGG | 0.475433 | 3.4:+1083562 | None:intergenic |
| CTGTTTAACCATACCTTCAG+TGG | 0.475898 | 3.4:+1083976 | None:intergenic |
| CGTCTGGTATTACTGGGGCC+AGG | 0.479425 | 3.4:+1088207 | None:intergenic |
| TCTGACGTATATATGCCATC+AGG | 0.484252 | 3.4:+1089515 | None:intergenic |
| AGAAATACAATTGGAAAGAA+AGG | 0.484703 | 3.4:-1089732 | MS.gene50848:CDS |
| CTTTATATATAGTCCCTTCA+CGG | 0.485953 | 3.4:+1089934 | None:intergenic |
| GGTGAATTCTCAACTTCTGC+AGG | 0.489502 | 3.4:-1083311 | MS.gene50848:CDS |
| GCCATCAGGGGTGTGACAAT+AGG | 0.490885 | 3.4:+1089529 | None:intergenic |
| TTCTGAAGGGGAATCTAAAA+GGG | 0.490928 | 3.4:-1083705 | MS.gene50848:CDS |
| GCAAACTCGAAAACATCATC+AGG | 0.492867 | 3.4:+1090679 | None:intergenic |
| CAGAACCAAAGGCGTGAAGC+CGG | 0.505752 | 3.4:-1083908 | MS.gene50848:CDS |
| ACAATGTCAAGAATGCTAAT+CGG | 0.510501 | 3.4:-1086491 | MS.gene50848:CDS |
| TCATATCCTGAATCAGTTGA+AGG | 0.510630 | 3.4:-1083503 | MS.gene50848:intron |
| AAGCCGGTGGAAGATTGATC+AGG | 0.514442 | 3.4:-1083892 | MS.gene50848:CDS |
| GGTGCCCAAACACCACGATC+AGG | 0.517553 | 3.4:+1083596 | None:intergenic |
| GAAGAATGGTCCTCACGCCT+GGG | 0.520144 | 3.4:+1086432 | None:intergenic |
| AACCCAGACCTTGAACCAAA+AGG | 0.525585 | 3.4:+1083767 | None:intergenic |
| TCCAATTGTATTTCTGCACT+CGG | 0.526926 | 3.4:+1089740 | None:intergenic |
| TCATCGGCCGCAACAAGAGT+TGG | 0.528330 | 3.4:-1088261 | MS.gene50848:CDS |
| GAAGGGAAACTTTCAAAGAA+AGG | 0.528593 | 3.4:-1083068 | MS.gene50848:CDS |
| TGCTGTTGACAGTGGTCCAC+TGG | 0.532809 | 3.4:-1089488 | MS.gene50848:intron |
| CCCTATATAGTGAAGGATGA+TGG | 0.537091 | 3.4:-1083107 | MS.gene50848:CDS |
| CTGCCTTTACTGTGGTCCCC+AGG | 0.539742 | 3.4:-1086449 | MS.gene50848:CDS |
| ACGCCTGGGGACCACAGTAA+AGG | 0.540090 | 3.4:+1086446 | None:intergenic |
| TGGTTGTCAGTATGTTCAGA+AGG | 0.540774 | 3.4:-1086514 | MS.gene50848:intron |
| CTCCTTTCTAGGGGCTAGCA+AGG | 0.541253 | 3.4:-1089557 | MS.gene50848:intron |
| TCAACTGATTCAGGATATGA+TGG | 0.541254 | 3.4:+1083506 | None:intergenic |
| ACCTATTGTCACACCCCTGA+TGG | 0.547863 | 3.4:-1089530 | MS.gene50848:CDS |
| ACTCAACTACTGCTTTATGC+TGG | 0.548532 | 3.4:+1090003 | None:intergenic |
| AGTAGTCCCTCTGTTGAGAA+TGG | 0.556313 | 3.4:-1083281 | MS.gene50848:intron |
| CTGACGTATATATGCCATCA+GGG | 0.556567 | 3.4:+1089516 | None:intergenic |
| CATGTCTCTAGTCATGATGC+TGG | 0.556919 | 3.4:-1083737 | MS.gene50848:CDS |
| CCTTCTGAAAGGAAAACAGC+GGG | 0.562206 | 3.4:-1084344 | MS.gene50848:CDS |
| AGAGCCAGAGCTGCTATGCA+AGG | 0.562280 | 3.4:-1088234 | MS.gene50848:CDS |
| TCTTAACTTCTGAGAATGGA+AGG | 0.562840 | 3.4:-1083802 | MS.gene50848:CDS |
| GTGCAGTCAGTTCGAGTGAA+GGG | 0.563790 | 3.4:-1083085 | MS.gene50848:CDS |
| GGCTTTGGAACACGCTGAGA+AGG | 0.570985 | 3.4:+1089975 | None:intergenic |
| TACTGTGGTCCCCAGGCGTG+AGG | 0.571346 | 3.4:-1086442 | MS.gene50848:CDS |
| TGGAACTTTCAGGCCACTGA+AGG | 0.573276 | 3.4:-1083989 | MS.gene50848:intron |
| TGGCCCCAGTAATACCAGAC+GGG | 0.576005 | 3.4:-1088205 | MS.gene50848:CDS |
| GCTGGGCATGCTAATTCTGA+AGG | 0.580615 | 3.4:-1083719 | MS.gene50848:CDS |
| GCTCCTGATCAATCTTCCAC+CGG | 0.582381 | 3.4:+1083889 | None:intergenic |
| TGAGCTCTCATCATCCTCAC+TGG | 0.585263 | 3.4:-1083540 | MS.gene50848:CDS |
| AGAAAGGAAGCTGAGCAAGC+TGG | 0.587029 | 3.4:-1089716 | MS.gene50848:intron |
| CACCAACCATTCTCAACAGA+GGG | 0.588071 | 3.4:+1083275 | None:intergenic |
| GCACTCGGAAGATGTTCTTG+AGG | 0.590407 | 3.4:+1089755 | None:intergenic |
| GGTGCAGTCAGTTCGAGTGA+AGG | 0.593819 | 3.4:-1083086 | MS.gene50848:CDS |
| CTGGGCATGCTAATTCTGAA+GGG | 0.594470 | 3.4:-1083718 | MS.gene50848:CDS |
| TCTTCTGCCAACTCTTGTTG+CGG | 0.594967 | 3.4:+1088254 | None:intergenic |
| ATGTCTCTAGTCATGATGCT+GGG | 0.599416 | 3.4:-1083736 | MS.gene50848:CDS |
| CTACTTCAAAACAGAACCAA+AGG | 0.603714 | 3.4:-1083919 | MS.gene50848:CDS |
| GAATTCTCAACTTCTGCAGG+TGG | 0.610336 | 3.4:-1083308 | MS.gene50848:CDS |
| GCCTATTCGGGAAACAGGAG+TGG | 0.612528 | 3.4:-1083332 | MS.gene50848:CDS |
| TTTCCTGAGTCAGAAGTCAG+TGG | 0.613047 | 3.4:+1084379 | None:intergenic |
| ATACCACTGACTTCTGACTC+AGG | 0.622853 | 3.4:-1084382 | MS.gene50848:CDS |
| CTGGCCCCAGTAATACCAGA+CGG | 0.624233 | 3.4:-1088206 | MS.gene50848:CDS |
| AACCAAAGGCGTGAAGCCGG+TGG | 0.625599 | 3.4:-1083905 | MS.gene50848:CDS |
| GACGTACTAGAAACAAGGAC+AGG | 0.626561 | 3.4:-1083619 | MS.gene50848:CDS |
| TGGGGCTCCCTATATAGTGA+AGG | 0.637270 | 3.4:-1083114 | MS.gene50848:CDS |
| ATTGATGAATATACAGTGCA+TGG | 0.638195 | 3.4:-1084427 | MS.gene50848:CDS |
| AACAAGGACAGGCCTGATCG+TGG | 0.639778 | 3.4:-1083608 | MS.gene50848:CDS |
| AGTCAGTGGTATTCCAGAGA+CGG | 0.644574 | 3.4:+1084393 | None:intergenic |
| CAGAAGCGTGCTGTTGACAG+TGG | 0.645146 | 3.4:-1089496 | MS.gene50848:CDS |
| TGGGCATGCTAATTCTGAAG+GGG | 0.646561 | 3.4:-1083717 | MS.gene50848:CDS |
| GACCTTGAACCAAAAGGACG+AGG | 0.650876 | 3.4:+1083773 | None:intergenic |
| TGTTGGGAATAGTACTCATG+AGG | 0.651580 | 3.4:-1083045 | MS.gene50848:CDS |
| AGAAAGACGTACTAGAAACA+AGG | 0.658009 | 3.4:-1083624 | MS.gene50848:CDS |
| AAGAATGGTCCTCACGCCTG+GGG | 0.661060 | 3.4:+1086433 | None:intergenic |
| TCCTTCTGAAAGGAAAACAG+CGG | 0.668874 | 3.4:-1084345 | MS.gene50848:CDS |
| CTTGAACCAAAAGGACGAGG+TGG | 0.668946 | 3.4:+1083776 | None:intergenic |
| GAAGAATCGGAACGGCGAAG+AGG | 0.673314 | 3.4:+1083575 | None:intergenic |
| ACACCAACCATTCTCAACAG+AGG | 0.678931 | 3.4:+1083274 | None:intergenic |
| AGACAAATCTGCCTTTACTG+TGG | 0.679939 | 3.4:-1086457 | MS.gene50848:CDS |
| GGCCCCAGTAATACCAGACG+GGG | 0.682231 | 3.4:-1088204 | MS.gene50848:CDS |
| TAGAATGTGAAGAATCGGAA+CGG | 0.692170 | 3.4:+1083567 | None:intergenic |
| GCAGTAGATGTAGACTGACT+CGG | 0.696602 | 3.4:+1083842 | None:intergenic |
| TGACGTATATATGCCATCAG+GGG | 0.713880 | 3.4:+1089517 | None:intergenic |
| ATCCCCGTCTGGTATTACTG+GGG | 0.722921 | 3.4:+1088202 | None:intergenic |
| TGGTGCAGACTGTGCCAGTG+AGG | 0.729152 | 3.4:+1083526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 1083028 | 1090747 | 1083028 | ID=MS.gene50848 |
| chr3.4 | mRNA | 1083028 | 1090747 | 1083028 | ID=MS.gene50848.t1;Parent=MS.gene50848 |
| chr3.4 | exon | 1090641 | 1090747 | 1090641 | ID=MS.gene50848.t1.exon1;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1090641 | 1090747 | 1090641 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1089934 | 1090029 | 1089934 | ID=MS.gene50848.t1.exon2;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1089934 | 1090029 | 1089934 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1089717 | 1089815 | 1089717 | ID=MS.gene50848.t1.exon3;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1089717 | 1089815 | 1089717 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1089489 | 1089568 | 1089489 | ID=MS.gene50848.t1.exon4;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1089489 | 1089568 | 1089489 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1088188 | 1088286 | 1088188 | ID=MS.gene50848.t1.exon5;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1088188 | 1088286 | 1088188 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1086394 | 1086526 | 1086394 | ID=MS.gene50848.t1.exon6;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1086394 | 1086526 | 1086394 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1084333 | 1084457 | 1084333 | ID=MS.gene50848.t1.exon7;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1084333 | 1084457 | 1084333 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1083504 | 1083999 | 1083504 | ID=MS.gene50848.t1.exon8;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1083504 | 1083999 | 1083504 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1083282 | 1083371 | 1083282 | ID=MS.gene50848.t1.exon9;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1083282 | 1083371 | 1083282 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| chr3.4 | exon | 1083028 | 1083161 | 1083028 | ID=MS.gene50848.t1.exon10;Parent=MS.gene50848.t1 |
| chr3.4 | CDS | 1083028 | 1083161 | 1083028 | ID=cds.MS.gene50848.t1;Parent=MS.gene50848.t1 |
| Gene Sequence |
| Protein sequence |
