AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50856


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50856.t1 MTR_3g005140 94.895 333 6 6 1 333 1 322 0.0 630
MS.gene50856.t1 MTR_7g011850 44.622 251 124 7 90 332 45 288 1.00e-57 188
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50856.t1 AT1G33780 65.490 255 76 4 79 332 70 313 5.18e-112 328
MS.gene50856.t1 AT3G29240 41.365 249 124 5 100 332 70 312 2.96e-54 180
MS.gene50856.t1 AT3G29240 41.365 249 124 5 100 332 70 312 2.96e-54 180

Find 0 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGAAAATATGGAAAA+CGG - 21095:70136-70155 MS.gene50856:intron 15.0%
!! TGAAAACATATTTGAAAATA+TGG - 21095:70129-70148 MS.gene50856:intron 15.0%
!! TTAAGGATTTATACAATAAA+TGG - 21095:70093-70112 MS.gene50856:intron 15.0%
!! TTATACATGATACATAATTA+TGG - 21095:69705-69724 MS.gene50856:intron 15.0%
!!! GATTTGTATCATTTTATTTA+TGG - 21095:69999-70018 MS.gene50856:intron 15.0%
!!! TTTTAAAAGCATAGTAAAAT+TGG + 21095:69185-69204 None:intergenic 15.0%
!! TATCGTCTAATGATTATTAT+TGG - 21095:69948-69967 MS.gene50856:intron 20.0%
!!! GTTACTAAATCATGATTTTT+AGG + 21095:70623-70642 None:intergenic 20.0%
! AAAAAGTTATAGGAAGTCAA+TGG - 21095:69668-69687 MS.gene50856:intron 25.0%
! AACAAGAAGAACAATAATCA+CGG - 21095:69052-69071 MS.gene50856:CDS 25.0%
! ACCAAGTTATAGAAAAATGA+AGG - 21095:69973-69992 MS.gene50856:intron 25.0%
! ATAGATACATAGAATGCAAA+AGG + 21095:70210-70229 None:intergenic 25.0%
! ATTATCATTAGTGCTTCTAT+GGG + 21095:69601-69620 None:intergenic 25.0%
! CAGAATAAAATGAATTGGTT+GGG + 21095:68907-68926 None:intergenic 25.0%
! CCAAGTTATAGAAAAATGAA+GGG - 21095:69974-69993 MS.gene50856:intron 25.0%
! CTTAATAACAAGTACAAACA+AGG + 21095:70078-70097 None:intergenic 25.0%
! GAGAGACTAAAAAAAGTTAT+AGG - 21095:69658-69677 MS.gene50856:intron 25.0%
! TGTTCCAGAATAAAATGAAT+TGG + 21095:68912-68931 None:intergenic 25.0%
! TTAATGACAAGTTCATCAAA+AGG + 21095:69868-69887 None:intergenic 25.0%
! TTGTTTACAGTTAATGATCT+CGG + 21095:68963-68982 None:intergenic 25.0%
!! GTATAATCATACTTGATAGT+TGG - 21095:69832-69851 MS.gene50856:intron 25.0%
!!! CTTGTTTGTACTTGTTATTA+AGG - 21095:70076-70095 MS.gene50856:intron 25.0%
ACAAAGAAACTGAAATCATG+AGG + 21095:70824-70843 None:intergenic 30.0%
AGATTAATCAACAACACAAC+AGG + 21095:69260-69279 None:intergenic 30.0%
ATGAAATTGAGTCAGAGTAT+TGG - 21095:70879-70898 MS.gene50856:CDS 30.0%
ATTGATGTAGAAGCTACTAT+TGG - 21095:70249-70268 MS.gene50856:intron 30.0%
CAAACCTCTTGAAACAAAAT+GGG - 21095:70415-70434 MS.gene50856:intron 30.0%
CATGATTTCAGTTTCTTTGT+TGG - 21095:70824-70843 MS.gene50856:CDS 30.0%
CATTATCATTAGTGCTTCTA+TGG + 21095:69602-69621 None:intergenic 30.0%
CCAGAAGGCAAAAATAATAA+TGG - 21095:69310-69329 MS.gene50856:CDS 30.0%
CCAGAATAAAATGAATTGGT+TGG + 21095:68908-68927 None:intergenic 30.0%
GAATGAAGTTATTCCAATTC+AGG + 21095:69512-69531 None:intergenic 30.0%
GAGAATAACAGTTCTTTCAA+AGG + 21095:70510-70529 None:intergenic 30.0%
GTTGATTAATCTAACCTTAC+AGG - 21095:69268-69287 MS.gene50856:CDS 30.0%
TAAACTATATAGAGATGAGC+TGG - 21095:69399-69418 MS.gene50856:CDS 30.0%
TAATCTAACCTTACAGGTAA+TGG - 21095:69274-69293 MS.gene50856:CDS 30.0%
TCATTCCATTGTAGAAAAGA+TGG - 21095:69526-69545 MS.gene50856:intron 30.0%
TGTTTAATCTTCTTGTGAAG+AGG + 21095:70593-70612 None:intergenic 30.0%
! CCAACCAATTCATTTTATTC+TGG - 21095:68905-68924 MS.gene50856:CDS 30.0%
!! AGCATGTTTTTGCTAAAATC+AGG - 21095:70689-70708 MS.gene50856:intron 30.0%
!! CATAGAAGCACTAATGATAA+TGG - 21095:69600-69619 MS.gene50856:intron 30.0%
!! CATTTTATTTATGGCTCGTT+TGG - 21095:70008-70027 MS.gene50856:intron 30.0%
!! CCCTTCATTTTTCTATAACT+TGG + 21095:69977-69996 None:intergenic 30.0%
!!! CCATTATTATTTTTGCCTTC+TGG + 21095:69313-69332 None:intergenic 30.0%
AAGAAGAACAATAATCACGG+CGG - 21095:69055-69074 MS.gene50856:CDS 35.0%
AATCCAGATTAACATGACCA+CGG + 21095:69363-69382 None:intergenic 35.0%
ACTGCATTGCTTCTTAGATA+TGG + 21095:70285-70304 None:intergenic 35.0%
CAATTAACCTTTATCCCACT+AGG + 21095:69759-69778 None:intergenic 35.0%
CCAAACCTCTTGAAACAAAA+TGG - 21095:70414-70433 MS.gene50856:intron 35.0%
GGGATAAAGGTTAATTGTCA+AGG - 21095:69762-69781 MS.gene50856:intron 35.0%
TAGAATGCAAAAGGCGAAAA+AGG + 21095:70201-70220 None:intergenic 35.0%
TAGACGATAAACACCTCAAT+GGG + 21095:69937-69956 None:intergenic 35.0%
TAGCAGTGACAACAAGAAAT+GGG + 21095:69015-69034 None:intergenic 35.0%
TTAATCAACAACACAACAGG+CGG + 21095:69257-69276 None:intergenic 35.0%
TTAGACGATAAACACCTCAA+TGG + 21095:69938-69957 None:intergenic 35.0%
TTAGCAGTGACAACAAGAAA+TGG + 21095:69016-69035 None:intergenic 35.0%
TTTACAGTTAATGATCTCGG+CGG + 21095:68960-68979 None:intergenic 35.0%
! AGATTCTTCTGAAGGTTTGT+GGG - 21095:70946-70965 MS.gene50856:CDS 35.0%
! AGTCTTGTCATAACCCATAT+AGG - 21095:69552-69571 MS.gene50856:intron 35.0%
! AGTTTCTTTGTTGGTTATGC+TGG - 21095:70833-70852 MS.gene50856:CDS 35.0%
! ATTAATCTAAGATGCCTCCT+TGG - 21095:69883-69902 MS.gene50856:intron 35.0%
! CGATTGATGACAATTCCAAA+AGG + 21095:70569-70588 None:intergenic 35.0%
! CTCTACCATCTTTTCTACAA+TGG + 21095:69534-69553 None:intergenic 35.0%
! GAGGAGATTTTGCAACTTAT+GGG - 21095:70968-70987 MS.gene50856:CDS 35.0%
! TCTGATTGTTCTCTGCATTT+TGG - 21095:70650-70669 MS.gene50856:intron 35.0%
!! AAAATTGGTTTGCAGGTTGA+TGG + 21095:69170-69189 None:intergenic 35.0%
!! CCATTTTGTTTCAAGAGGTT+TGG + 21095:70417-70436 None:intergenic 35.0%
!! GAAGCACTAATGATAATGGT+TGG - 21095:69604-69623 MS.gene50856:intron 35.0%
!! GCATAGTAAAATTGGTTTGC+AGG + 21095:69177-69196 None:intergenic 35.0%
!! GTTATTCTCCTTCTAAGATC+TGG - 21095:70521-70540 MS.gene50856:CDS 35.0%
!! TAGCTCAAGTTTGCTGTATA+AGG - 21095:70916-70935 MS.gene50856:CDS 35.0%
AAAAACATGCTTGCCTCAAG+AGG + 21095:70680-70699 None:intergenic 40.0%
AACTGTTTAGATGATGCAGC+AGG - 21095:70773-70792 MS.gene50856:intron 40.0%
AATAACAACTCTGACAACCG+TGG - 21095:69343-69362 MS.gene50856:CDS 40.0%
ACATGTCCAAACCACCTATA+TGG + 21095:69569-69588 None:intergenic 40.0%
ACTGTTTAGATGATGCAGCA+GGG - 21095:70774-70793 MS.gene50856:intron 40.0%
ATGCAGAGAACAATCAGAGA+AGG + 21095:70648-70667 None:intergenic 40.0%
CATAACCCATATAGGTGGTT+TGG - 21095:69560-69579 MS.gene50856:intron 40.0%
CATGTCCAAACCACCTATAT+GGG + 21095:69568-69587 None:intergenic 40.0%
CTTGTCATAACCCATATAGG+TGG - 21095:69555-69574 MS.gene50856:intron 40.0%
GAGCTCCATAATATAGACCA+GGG + 21095:70751-70770 None:intergenic 40.0%
GCTTTGACAGATTCTTCTGA+AGG - 21095:70938-70957 MS.gene50856:CDS 40.0%
GTAGCAACTTACTCGATGTT+AGG - 21095:70303-70322 MS.gene50856:intron 40.0%
GTAGTCCCTGGTCTATATTA+TGG - 21095:70743-70762 MS.gene50856:intron 40.0%
GTGGTCATGTTAATCTGGAT+TGG - 21095:69362-69381 MS.gene50856:CDS 40.0%
TCAGAAGTCCAAGAGTTAAG+AGG - 21095:69631-69650 MS.gene50856:intron 40.0%
TCTCTCTACCTCTTAACTCT+TGG + 21095:69642-69661 None:intergenic 40.0%
TGACAACAAGAAATGGGATG+CGG + 21095:69009-69028 None:intergenic 40.0%
TGTACCTCTGTCTGTTTAAG+TGG - 21095:69473-69492 MS.gene50856:CDS 40.0%
TTAACCTTTATCCCACTAGG+TGG + 21095:69756-69775 None:intergenic 40.0%
TTCGAGTCAACTTCCTGAAT+TGG - 21095:69496-69515 MS.gene50856:intron 40.0%
! ATAGGTGGTTTGGACATGTT+TGG - 21095:69570-69589 MS.gene50856:intron 40.0%
! CAGATTCTTCTGAAGGTTTG+TGG - 21095:70945-70964 MS.gene50856:CDS 40.0%
! GATTGTTCTCTGCATTTTGG+TGG - 21095:70653-70672 MS.gene50856:intron 40.0%
! GGAGGAGATTTTGCAACTTA+TGG - 21095:70967-70986 MS.gene50856:CDS 40.0%
! GTCTAGTTCCAGATCTTAGA+AGG + 21095:70532-70551 None:intergenic 40.0%
! TCTTTGTTGGTTATGCTGGA+TGG - 21095:70837-70856 MS.gene50856:CDS 40.0%
!! TGAGCCCATTTTGTTTCAAG+AGG + 21095:70422-70441 None:intergenic 40.0%
!! TGAGTCAGAGTATTGGTATG+TGG - 21095:70886-70905 MS.gene50856:CDS 40.0%
!!! TTTTGCCTTCTGGAATAGAC+TGG + 21095:69303-69322 None:intergenic 40.0%
AAGAACAATAATCACGGCGG+AGG - 21095:69058-69077 MS.gene50856:CDS 45.0%
ACTGGTCACCATTACCTGTA+AGG + 21095:69285-69304 None:intergenic 45.0%
ATTCAGAAGACTCCACCTAG+TGG - 21095:69741-69760 MS.gene50856:intron 45.0%
ATTCCAAAAGGCCCTTCTTG+TGG + 21095:70557-70576 None:intergenic 45.0%
CAACCGTGGTCATGTTAATC+TGG - 21095:69357-69376 MS.gene50856:CDS 45.0%
CAGAGAACAATCAGAGAAGG+TGG + 21095:70645-70664 None:intergenic 45.0%
CGAACCACTTAAACAGACAG+AGG + 21095:69480-69499 None:intergenic 45.0%
CGAGCTCCATAATATAGACC+AGG + 21095:70752-70771 None:intergenic 45.0%
GAACTAGACACCCACAAGAA+GGG - 21095:70543-70562 MS.gene50856:CDS 45.0%
GAAGCTTCCAGGATTTGAAG+AGG - 21095:70721-70740 MS.gene50856:intron 45.0%
GATGCCGATACACACAAAGA+AGG - 21095:70374-70393 MS.gene50856:intron 45.0%
GCGAATACCATCAAGCTTGT+CGG + 21095:70486-70505 None:intergenic 45.0%
TGACTGTATCTGTCCCATTG+AGG - 21095:69921-69940 MS.gene50856:intron 45.0%
TGGTTATGCTGGATGGCAAA+TGG - 21095:70844-70863 MS.gene50856:CDS 45.0%
TTCAGAAGACTCCACCTAGT+GGG - 21095:69742-69761 MS.gene50856:intron 45.0%
TTCCAAAAGGCCCTTCTTGT+GGG + 21095:70556-70575 None:intergenic 45.0%
TTTGCAACTTATGGGTACGC+AGG - 21095:70976-70995 MS.gene50856:CDS 45.0%
! TTCTTCTGAAGGTTTGTGGG+AGG - 21095:70949-70968 MS.gene50856:CDS 45.0%
AACAATAATCACGGCGGAGG+TGG - 21095:69061-69080 MS.gene50856:CDS 50.0%
ACAAGTACACAGCCAGTCTC+AGG + 21095:70458-70477 None:intergenic 50.0%
CAACAAGAAATGGGATGCGG+CGG + 21095:69006-69025 None:intergenic 50.0%
CATGTTGTCAGCCTCAACCA+AGG + 21095:69903-69922 None:intergenic 50.0%
CCAGTCTCAGGTACAGGAAT+AGG + 21095:70446-70465 None:intergenic 50.0%
CCCGCCTTCTTTGTGTGTAT+CGG + 21095:70381-70400 None:intergenic 50.0%
CCGATACACACAAAGAAGGC+GGG - 21095:70378-70397 MS.gene50856:intron 50.0%
CCTATTCCTGTACCTGAGAC+TGG - 21095:70443-70462 MS.gene50856:intron 50.0%
CGATACACACAAAGAAGGCG+GGG - 21095:70379-70398 MS.gene50856:intron 50.0%
CTAAGATGCCTCCTTGGTTG+AGG - 21095:69889-69908 MS.gene50856:intron 50.0%
CTTCTTGTTCTTCTTGCCGC+CGG + 21095:69041-69060 None:intergenic 50.0%
GACTCCACCTAGTGGGATAA+AGG - 21095:69749-69768 MS.gene50856:intron 50.0%
GCCGATACACACAAAGAAGG+CGG - 21095:70377-70396 MS.gene50856:intron 50.0%
GGAACTAGACACCCACAAGA+AGG - 21095:70542-70561 MS.gene50856:CDS 50.0%
GGAGAGAAGTTGAAGCTTCC+AGG - 21095:70710-70729 MS.gene50856:intron 50.0%
GGATTTGAAGAGGTAGTCCC+TGG - 21095:70731-70750 MS.gene50856:intron 50.0%
GGGACTACCTCTTCAAATCC+TGG + 21095:70731-70750 None:intergenic 50.0%
GGTGACCAGTCTATTCCAGA+AGG - 21095:69295-69314 MS.gene50856:CDS 50.0%
! CTTGTTGTCACTGCTAACGC+CGG - 21095:69019-69038 MS.gene50856:CDS 50.0%
! TGAGACTGGCTGTGTACTTG+TGG - 21095:70457-70476 MS.gene50856:CDS 50.0%
!! GCATTTTGGTGGACCTCTTG+AGG - 21095:70664-70683 MS.gene50856:intron 50.0%
AATAATCACGGCGGAGGTGG+AGG - 21095:69064-69083 MS.gene50856:CDS 55.0%
ACACAGCCAGTCTCAGGTAC+AGG + 21095:70452-70471 None:intergenic 55.0%
CAGCAGGGCTTGTGAAGAAG+GGG - 21095:70789-70808 MS.gene50856:CDS 55.0%
GCAGCAGGGCTTGTGAAGAA+GGG - 21095:70788-70807 MS.gene50856:CDS 55.0%
GTGGCTACCGACAAGCTTGA+TGG - 21095:70476-70495 MS.gene50856:CDS 55.0%
GTTGTCAGCCTCAACCAAGG+AGG + 21095:69900-69919 None:intergenic 55.0%
TGCAGCAGGGCTTGTGAAGA+AGG - 21095:70787-70806 MS.gene50856:CDS 55.0%
! CACCCACAAGAAGGGCCTTT+TGG - 21095:70551-70570 MS.gene50856:CDS 55.0%
GTTGTCACTGCTAACGCCGG+CGG - 21095:69022-69041 MS.gene50856:CDS 60.0%
AATCACGGCGGAGGTGGAGG+AGG - 21095:69067-69086 MS.gene50856:CDS 65.0%
CACGGCGGAGGTGGAGGAGG+AGG - 21095:69070-69089 MS.gene50856:CDS 75.0%


Chromosome Type Strat End Strand Name
21095 gene 68863 71003 68863 ID=MS.gene50856
21095 mRNA 68863 71003 68863 ID=MS.gene50856.t1;Parent=MS.gene50856
21095 exon 70775 71003 70775 ID=MS.gene50856.t1.exon1;Parent=MS.gene50856.t1
21095 CDS 70775 71003 70775 ID=cds.MS.gene50856.t1;Parent=MS.gene50856.t1
21095 exon 70446 70576 70446 ID=MS.gene50856.t1.exon2;Parent=MS.gene50856.t1
21095 CDS 70446 70576 70446 ID=cds.MS.gene50856.t1;Parent=MS.gene50856.t1
21095 exon 68863 69504 68863 ID=MS.gene50856.t1.exon3;Parent=MS.gene50856.t1
21095 CDS 68863 69504 68863 ID=cds.MS.gene50856.t1;Parent=MS.gene50856.t1
Gene Sequence

>MS.gene50856

ATGGATCTGTGTGGCGTTAATGTGAAGAACAATCTTTTGTACCCAACCAATTCATTTTATTCTGGAACAACAAGATCATCATCTTTTTACAACGCCGCCGAGATCATTAACTGTAAACAACAACAACAACACTTTCAACGCCGCCGCATCCCATTTCTTGTTGTCACTGCTAACGCCGGCGGCAAGAAGAACAAGAAGAACAATAATCACGGCGGAGGTGGAGGAGGAGGTATGTATACTCTTCATTTCTTGTTGTCACTCACTGCATTTGCATTATTATTTATACTTGTAAGTAATTCTCTATCCATCAACCTGCAAACCAATTTTACTATGCTTTTAAAACATTCAGTCAATGTCATCATGCTTCTAATTTACTGTTTTACAAGTATAGCCGCCTGTTGTGTTGTTGATTAATCTAACCTTACAGGTAATGGTGACCAGTCTATTCCAGAAGGCAAAAATAATAATGGTGATAAATCAAATAACAACTCTGACAACCGTGGTCATGTTAATCTGGATTGGCGTGAATTCAGAGCTAAACTATATAGAGATGAGCTGGTAGTAATCAATTCATTGCTAAATAAATACAATCTGTCTGTAATTTACATGATGTACCTCTGTCTGTTTAAGTGGTTCGAGTCAACTTCCTGAATTGGAATAACTTCATTCCATTGTAGAAAAGATGGTAGAGTCTTGTCATAACCCATATAGGTGGTTTGGACATGTTTGGAAAAACCCATAGAAGCACTAATGATAATGGTTGGTTGATCAGAAGTCCAAGAGTTAAGAGGTAGAGAGAGACTAAAAAAAGTTATAGGAAGTCAATGGTCTATTTGTGAACTTTATACATGATACATAATTATGGCATTGTTTGATTCATTCAGAAGACTCCACCTAGTGGGATAAAGGTTAATTGTCAAGGTTTTAAAACATCTGTTATATCTCTTTATGTGATTTGTTTCAGCAGTAGTATAATCATACTTGATAGTTGGAGATTCATCTCCTTTTGATGAACTTGTCATTAATCTAAGATGCCTCCTTGGTTGAGGCTGACAACATGACTGTATCTGTCCCATTGAGGTGTTTATCGTCTAATGATTATTATTGGTGACCAAGTTATAGAAAAATGAAGGGTTGATTTGTATCATTTTATTTATGGCTCGTTTGGTTTAAGAAAATGTGTTTTATTTTCACTAATATTTACAAATACTACCTTGTTTGTACTTGTTATTAAGGATTTATACAATAAATGGTGAAAATAGAAAATGAAAACATATTTGAAAATATGGAAAACGGAAACTGACTTTTTTTCATAATTATTAATGAAAAAAAAATCCTTTTTCGCCTTTTGCATTCTATGTATCTATACTTGATGTACTTCTAATTATTGATGTAGAAGCTACTATTGGTTATGATTGACCATATCTAAGAAGCAATGCAGTAGCAACTTACTCGATGTTAGGTTTCATTAACTGAGTTTTTCTTGACTTAATTAACAGAAAGAAATAGCAGATGCCGATACACACAAAGAAGGCGGGGCATTACACATTTCCAAACCTCTTGAAACAAAATGGGCTCATCCTATTCCTGTACCTGAGACTGGCTGTGTACTTGTGGCTACCGACAAGCTTGATGGTATTCGCACCTTTGAAAGAACTGTTATTCTCCTTCTAAGATCTGGAACTAGACACCCACAAGAAGGGCCTTTTGGAATTGTCATCAATCGTCCTCTTCACAAGAAGATTAAACAGATGAATCCTAAAAATCATGATTTAGTAACCACCTTCTCTGATTGTTCTCTGCATTTTGGTGGACCTCTTGAGGCAAGCATGTTTTTGCTAAAATCAGGAGAGAAGTTGAAGCTTCCAGGATTTGAAGAGGTAGTCCCTGGTCTATATTATGGAGCTCGAAACTGTTTAGATGATGCAGCAGGGCTTGTGAAGAAGGGGATTATCAAGCCTCATGATTTCAGTTTCTTTGTTGGTTATGCTGGATGGCAAATGGATCAGTTGCGAGATGAAATTGAGTCAGAGTATTGGTATGTGGCTGCATGTAGCTCAAGTTTGCTGTATAAGGCTTTGACAGATTCTTCTGAAGGTTTGTGGGAGGAGATTTTGCAACTTATGGGTACGCAGGATTGA

Protein sequence

>MS.gene50856.t1

MDLCGVNVKNNLLYPTNSFYSGTTRSSSFYNAAEIINCKQQQQHFQRRRIPFLVVTANAGGKKNKKNNNHGGGGGGGNGDQSIPEGKNNNGDKSNNNSDNRGHVNLDWREFRAKLYRDELKEIADADTHKEGGALHISKPLETKWAHPIPVPETGCVLVATDKLDGIRTFERTVILLLRSGTRHPQEGPFGIVINRPLHKKIKQMNPKNHDLVTTFSDCSLHFGGPLEASMFLLKSGEKLKLPGFEEVVPGLYYGARNCLDDAAGLVKKGIIKPHDFSFFVGYAGWQMDQLRDEIESEYWYVAACSSSLLYKALTDSSEGLWEEILQLMGTQD