AlfalfaGEDB | MS.gene50928

M. sativa cultivar XinJiangDaYe / MS.gene50928

KEGG: -
GO: -
PFAM: PF18052.4

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50928.t1	RHN65936.1	88.9	81	9	0	1	81	1	81	1.90E-28	134.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50928.t1	Q9LRR4	40.2	82	44	2	1	81	4	81	3.5e-07	55.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50928.t1	A0A072TQW6	88.9	81	9	0	1	81	1	81	1.4e-28	134.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049264	MS.gene50928	0.801949	7.07E-49	-1.69E-46
MS.gene050807	MS.gene50928	0.804791	1.82E-49	-1.69E-46
MS.gene050826	MS.gene50928	0.806662	7.38E-50	-1.69E-46
MS.gene051800	MS.gene50928	0.802256	6.12E-49	-1.69E-46
MS.gene052673	MS.gene50928	-0.817441	3.29E-52	-1.69E-46
MS.gene054379	MS.gene50928	0.807907	4.02E-50	-1.69E-46
MS.gene054467	MS.gene50928	0.813851	2.08E-51	-1.69E-46
MS.gene054468	MS.gene50928	0.822948	1.80E-53	-1.69E-46
MS.gene057869	MS.gene50928	0.81565	8.29E-52	-1.69E-46
MS.gene059438	MS.gene50928	0.805343	1.40E-49	-1.69E-46
MS.gene059439	MS.gene50928	0.841407	4.84E-58	-1.69E-46
MS.gene06052	MS.gene50928	0.82516	5.46E-54	-1.69E-46
MS.gene060998	MS.gene50928	0.804026	2.63E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50928.t1	MTR_3g023030	87.952	83	8	1	1	81	1	83	4.30e-40	140
MS.gene50928.t1	MTR_3g023030	87.952	83	8	1	1	81	1	83	4.51e-40	140
MS.gene50928.t1	MTR_3g022300	88.889	81	9	0	1	81	1	81	5.28e-40	139
MS.gene50928.t1	MTR_0425s0020	88.889	81	9	0	1	81	1	81	2.81e-39	137
MS.gene50928.t1	MTR_3g022600	86.420	81	11	0	1	81	1	81	1.33e-38	135
MS.gene50928.t1	MTR_3g027200	85.185	81	12	0	1	81	1	81	1.38e-38	135
MS.gene50928.t1	MTR_3g022130	83.951	81	13	0	1	81	1	81	1.12e-37	133
MS.gene50928.t1	MTR_3g022230	79.012	81	17	0	1	81	1	81	4.79e-34	122
MS.gene50928.t1	MTR_3g019040	62.195	82	30	1	1	81	1	82	4.88e-25	97.1
MS.gene50928.t1	MTR_3g018980	58.537	82	33	1	1	81	1	82	7.56e-23	90.9
MS.gene50928.t1	MTR_3g018930	58.537	82	33	1	1	81	3	84	1.12e-22	90.5
MS.gene50928.t1	MTR_3g018980	58.537	82	33	1	1	81	1	82	1.14e-22	90.5
MS.gene50928.t1	MTR_3g018930	58.537	82	33	1	1	81	3	84	1.99e-22	89.7
MS.gene50928.t1	MTR_3g027420	58.025	81	30	1	1	81	1	77	5.54e-22	88.6
MS.gene50928.t1	MTR_7g021000	55.556	81	32	1	1	81	1	77	1.68e-21	87.0
MS.gene50928.t1	MTR_3g025360	59.494	79	28	1	1	79	1	75	5.45e-21	85.5
MS.gene50928.t1	MTR_7g021000	55.556	81	32	1	1	81	1	77	6.95e-21	85.5
MS.gene50928.t1	MTR_3g015260	60.976	82	31	1	1	81	3	84	1.53e-20	84.3
MS.gene50928.t1	MTR_3g015260	60.976	82	31	1	1	81	3	84	1.55e-20	84.3
MS.gene50928.t1	MTR_3g022400	56.962	79	30	1	1	79	1	75	9.91e-20	82.0
MS.gene50928.t1	MTR_3g022400	56.962	79	30	1	1	79	1	75	1.03e-19	82.0
MS.gene50928.t1	MTR_3g019000	53.659	82	37	1	1	81	1	82	5.89e-19	79.7
MS.gene50928.t1	MTR_3g027250	51.852	81	35	1	1	81	1	77	1.24e-18	79.0
MS.gene50928.t1	MTR_3g022940	55.128	78	31	1	4	81	1	74	2.34e-17	75.1
MS.gene50928.t1	MTR_3g032960	43.210	81	45	1	2	81	3	83	5.16e-17	74.3
MS.gene50928.t1	MTR_3g021100	51.220	82	35	2	1	81	1	78	5.57e-17	74.3
MS.gene50928.t1	MTR_3g022790	48.148	81	38	1	1	81	1	77	1.34e-16	73.2
MS.gene50928.t1	MTR_3g032200	44.444	81	44	1	2	81	3	83	3.13e-16	72.0
MS.gene50928.t1	MTR_3g032500	44.444	81	44	1	2	81	3	83	3.42e-16	72.0
MS.gene50928.t1	MTR_3g032340	41.975	81	46	1	2	81	3	83	3.84e-16	72.0
MS.gene50928.t1	MTR_3g033380	44.444	81	44	1	2	81	3	83	4.72e-16	71.6
MS.gene50928.t1	MTR_0083s0110	40.741	81	47	1	2	81	3	83	6.39e-16	68.6
MS.gene50928.t1	MTR_3g031940	41.975	81	46	1	2	81	3	83	1.40e-15	70.1
MS.gene50928.t1	MTR_3g030960	40.741	81	47	1	2	81	3	83	1.53e-15	70.1
MS.gene50928.t1	MTR_3g030980	40.741	81	47	1	2	81	3	83	2.06e-15	69.7
MS.gene50928.t1	MTR_8g030860	40.741	81	47	1	2	81	3	83	3.41e-15	69.3
MS.gene50928.t1	MTR_6g084550	44.444	81	44	1	2	81	3	83	6.34e-15	68.2
MS.gene50928.t1	MTR_3g032370	41.026	78	45	1	5	81	4	81	7.97e-15	68.2
MS.gene50928.t1	MTR_3g035960	44.444	81	44	1	2	81	3	83	8.11e-15	68.2
MS.gene50928.t1	MTR_3g032800	39.506	81	48	1	2	81	3	83	1.07e-14	67.8
MS.gene50928.t1	MTR_8g038590	45.000	80	43	1	3	81	4	83	1.61e-14	67.4
MS.gene50928.t1	MTR_3g032150	41.026	78	45	1	5	81	4	81	1.77e-14	67.0
MS.gene50928.t1	MTR_3g032110	38.272	81	49	1	2	81	3	83	1.93e-14	67.0
MS.gene50928.t1	MTR_3g033240	39.506	81	48	1	2	81	3	83	2.91e-14	66.6
MS.gene50928.t1	MTR_3g022870	46.667	75	32	1	7	81	8	74	4.54e-14	65.9
MS.gene50928.t1	MTR_3g022870	46.667	75	32	1	7	81	8	74	4.87e-14	65.9
MS.gene50928.t1	MTR_3g021380	48.649	74	34	1	6	79	10	79	5.26e-14	65.9
MS.gene50928.t1	MTR_1g047800	41.772	79	46	0	3	81	4	82	5.73e-14	65.5
MS.gene50928.t1	MTR_3g014360	42.683	82	46	1	1	81	1	82	6.74e-14	65.5
MS.gene50928.t1	MTR_3g014530	45.570	79	42	1	4	81	1	79	8.01e-14	62.8
MS.gene50928.t1	MTR_3g022920	48.148	81	38	2	1	81	1	77	1.14e-13	64.7
MS.gene50928.t1	MTR_3g035810	48.750	80	36	2	1	79	1	76	1.43e-13	64.7
MS.gene50928.t1	MTR_4g050410	43.210	81	45	1	2	81	3	83	1.56e-13	64.3
MS.gene50928.t1	MTR_0003s0170	40.789	76	44	1	7	81	6	81	1.75e-13	63.5
MS.gene50928.t1	MTR_3g435720	38.272	81	49	1	2	81	3	83	3.11e-13	63.5
MS.gene50928.t1	MTR_3g021170	45.122	82	40	2	1	81	1	78	5.05e-13	62.8
MS.gene50928.t1	MTR_8g038570	42.500	80	45	1	3	81	4	83	8.41e-13	62.4
MS.gene50928.t1	MTR_3g035840	45.679	81	43	1	2	81	3	83	1.91e-12	61.2
MS.gene50928.t1	MTR_3g021340	46.341	82	39	2	1	81	1	78	2.16e-12	61.2
MS.gene50928.t1	MTR_3g028040	38.272	81	49	1	2	81	3	83	2.69e-12	60.8
MS.gene50928.t1	MTR_2g063777	35.802	81	51	1	2	81	3	83	3.64e-12	60.5
MS.gene50928.t1	MTR_3g435740	39.506	81	48	1	2	81	48	128	8.51e-12	59.3
MS.gene50928.t1	MTR_5g095910	39.759	83	48	2	1	81	1	83	9.74e-12	59.3
MS.gene50928.t1	MTR_3g033080	39.506	81	48	1	2	81	3	83	1.00e-11	59.3
MS.gene50928.t1	MTR_2g014730	38.462	78	47	1	5	81	6	83	1.44e-11	58.9
MS.gene50928.t1	MTR_3g033160	39.080	87	48	2	4	85	34	120	1.90e-11	55.8
MS.gene50928.t1	MTR_3g014510	40.244	82	48	1	1	81	1	82	1.99e-11	58.5
MS.gene50928.t1	MTR_3g020890	40.964	83	47	2	1	81	1	83	2.88e-11	58.2
MS.gene50928.t1	MTR_0005s0200	41.333	75	43	1	8	81	27	101	3.16e-11	57.8
MS.gene50928.t1	MTR_3g033110	38.272	81	49	1	2	81	3	83	3.27e-11	57.8
MS.gene50928.t1	MTR_3g021400	45.946	74	36	1	8	81	2	71	3.56e-11	57.8
MS.gene50928.t1	MTR_2g039090	36.250	80	51	0	2	81	3	82	4.78e-11	57.4
MS.gene50928.t1	MTR_3g014040	45.679	81	43	1	2	81	3	83	5.80e-11	57.0
MS.gene50928.t1	MTR_3g035940	44.444	81	44	1	2	81	3	83	9.65e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50928.t1	AT3G14470	40.244	82	44	2	1	81	4	81	2.28e-11	58.2
MS.gene50928.t1	AT3G14470	40.244	82	44	2	1	81	4	81	2.28e-11	58.2

Find 16 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGATATCAGAAACTATTTC+AGG	0.281058	3.3:-13824540	MS.gene50928:CDS
ACTTCTTCACAAGTGGGATT+TGG	0.351932	3.3:+13824428	None:intergenic
TCAAGTGATTATTGAGAAGT+TGG	0.395437	3.3:-13824569	MS.gene50928:CDS
TCTCTGCTTCGTCCAGTACT+AGG	0.396690	3.3:+13824461	None:intergenic
TCAATATATCATGGCAGAAT+TGG	0.426216	3.3:-13824620	None:intergenic
ACTTAAACATGTTGTTTATG+AGG	0.457103	3.3:-13824395	MS.gene50928:CDS
ATCATGGCAGAATTGGTAGC+TGG	0.460957	3.3:-13824613	MS.gene50928:CDS
TGATTCTATCAACCTAGTAC+TGG	0.500010	3.3:-13824473	MS.gene50928:CDS
TCATGGCAGAATTGGTAGCT+GGG	0.516366	3.3:-13824612	MS.gene50928:CDS
TCAAGCCACTTCTTCACAAG+TGG	0.549955	3.3:+13824421	None:intergenic
AAATCCCACTTGTGAAGAAG+TGG	0.556326	3.3:-13824426	MS.gene50928:CDS
CTTCTCAATAATCACTTGAA+AGG	0.569756	3.3:+13824573	None:intergenic
GAAATAGTTTCTGATATCAA+CGG	0.592464	3.3:+13824543	None:intergenic
CATGGCAGAATTGGTAGCTG+GGG	0.616301	3.3:-13824611	MS.gene50928:CDS
CAAGCCACTTCTTCACAAGT+GGG	0.622654	3.3:+13824422	None:intergenic
TAATTTAAAAGATTAACTTG+AGG	0.641641	3.3:+13824368	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAAATAGTTTCTGATATCAA+CGG	+	chr3.3:13824442-13824461	None:intergenic	25.0%
!	TTGATATCAGAAACTATTTC+AGG	-	chr3.3:13824442-13824461	MS.gene50928:CDS	25.0%
!!	ACTTAAACATGTTGTTTATG+AGG	-	chr3.3:13824587-13824606	MS.gene50928:CDS	25.0%
	CTTCTCAATAATCACTTGAA+AGG	+	chr3.3:13824412-13824431	None:intergenic	30.0%
	TCAAGTGATTATTGAGAAGT+TGG	-	chr3.3:13824413-13824432	MS.gene50928:CDS	30.0%
	TGATTCTATCAACCTAGTAC+TGG	-	chr3.3:13824509-13824528	MS.gene50928:CDS	35.0%
	AAATCCCACTTGTGAAGAAG+TGG	-	chr3.3:13824556-13824575	MS.gene50928:CDS	40.0%
	ACTTCTTCACAAGTGGGATT+TGG	+	chr3.3:13824557-13824576	None:intergenic	40.0%
	CAAGCCACTTCTTCACAAGT+GGG	+	chr3.3:13824563-13824582	None:intergenic	45.0%
	TCAAGCCACTTCTTCACAAG+TGG	+	chr3.3:13824564-13824583	None:intergenic	45.0%
	TCTCTGCTTCGTCCAGTACT+AGG	+	chr3.3:13824524-13824543	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	13824372	13824632	13824372	ID=MS.gene50928
chr3.3	mRNA	13824372	13824632	13824372	ID=MS.gene50928.t1;Parent=MS.gene50928
chr3.3	exon	13824372	13824632	13824372	ID=MS.gene50928.t1.exon1;Parent=MS.gene50928.t1
chr3.3	CDS	13824372	13824632	13824372	ID=cds.MS.gene50928.t1;Parent=MS.gene50928.t1

Gene Sequence	>MS.gene50928 ATGGCAGAATTGGTAGCTGGGGCATTTCTTCAATCTTCCTTTCAAGTGATTATTGAGAAGTTGGCTTCCGTTGATATCAGAAACTATTTCAGGAGTAACAATGTTGATGAACTTGTGAAAGAACTTCATACTGCACTTGATTCTATCAACCTAGTACTGGACGAAGCAGAGATAAAGCAGTACCAAATCCCACTTGTGAAGAAGTGGCTTGATGAACTTAAACATGTTGTTTATGAGGCAGACCTCAAGTTAATCTTTTAA
Protein sequence	>MS.gene50928.t1 MAELVAGAFLQSSFQVIIEKLASVDIRNYFRSNNVDELVKELHTALDSINLVLDEAEIKQYQIPLVKKWLDELKHVVYEADLKLIF