AlfalfaGEDB | MS.gene51560

M. sativa cultivar XinJiangDaYe / MS.gene51560

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51560.t1	XP_004498649.1	98	100	2	0	14	113	35	134	1.00E-45	192.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51560.t1	Q8H7Y8	94.0	100	6	0	14	113	39	138	5.0e-46	184.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51560.t1	G7I6D5	98.0	100	2	0	14	113	35	134	7.2e-46	192.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene51560	0.814543	1.46E-51	-1.69E-46
MS.gene050916	MS.gene51560	0.833594	4.88E-56	-1.69E-46
MS.gene051330	MS.gene51560	0.804501	2.10E-49	-1.69E-46
MS.gene051903	MS.gene51560	0.82756	1.46E-54	-1.69E-46
MS.gene052947	MS.gene51560	0.80556	1.26E-49	-1.69E-46
MS.gene053401	MS.gene51560	0.81405	1.88E-51	-1.69E-46
MS.gene055808	MS.gene51560	0.818215	2.20E-52	-1.69E-46
MS.gene056631	MS.gene51560	0.825421	4.73E-54	-1.69E-46
MS.gene057705	MS.gene51560	0.816899	4.36E-52	-1.69E-46
MS.gene06001	MS.gene51560	0.82942	5.20E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51560.t1	MTR_1g011750	98.000	100	2	0	14	113	35	134	1.26e-66	197
MS.gene51560.t1	MTR_1g011850	98.000	100	2	0	14	113	35	134	1.26e-66	197
MS.gene51560.t1	MTR_1g071040	90.000	100	10	0	14	113	35	134	1.65e-60	181
MS.gene51560.t1	MTR_1g114050	86.000	100	14	0	14	113	32	131	2.92e-57	173
MS.gene51560.t1	MTR_4g048490	83.582	67	11	0	47	113	39	105	9.70e-35	115
MS.gene51560.t1	MTR_7g108320	60.825	97	36	2	14	109	31	126	2.10e-33	113
MS.gene51560.t1	MTR_7g114060	60.825	97	36	2	14	109	28	123	3.83e-33	112
MS.gene51560.t1	MTR_7g114040	60.825	97	36	2	14	109	28	123	3.83e-33	112
MS.gene51560.t1	MTR_2g082220	60.606	99	37	2	14	111	25	122	1.27e-32	111
MS.gene51560.t1	MTR_2g082510	60.606	99	37	2	14	111	25	122	1.34e-32	111
MS.gene51560.t1	MTR_7g093910	58.763	97	38	2	14	109	31	126	1.12e-31	109
MS.gene51560.t1	MTR_8g086640	58.416	101	40	2	14	113	33	132	4.59e-30	105
MS.gene51560.t1	MTR_4g071150	59.000	100	39	2	14	112	33	131	8.26e-30	104
MS.gene51560.t1	MTR_7g113160	54.545	99	43	2	14	111	35	132	8.99e-30	104
MS.gene51560.t1	MTR_4g064967	57.426	101	41	2	14	113	33	132	1.28e-29	103
MS.gene51560.t1	MTR_4g064005	57.426	101	41	2	14	113	33	132	1.35e-29	103
MS.gene51560.t1	MTR_2g096570	58.000	100	40	2	14	112	32	130	1.46e-29	103
MS.gene51560.t1	MTR_4g063280	57.426	101	41	2	14	113	27	126	1.58e-29	103
MS.gene51560.t1	MTR_2g096610	57.426	101	41	2	14	113	31	130	1.77e-29	103
MS.gene51560.t1	MTR_4g063410	58.763	97	38	2	14	109	33	128	8.11e-29	102
MS.gene51560.t1	MTR_7g027610	55.000	100	21	1	14	113	23	98	1.04e-25	93.2
MS.gene51560.t1	MTR_0653s0030	87.500	48	6	0	44	91	50	97	1.30e-22	86.7
MS.gene51560.t1	MTR_7g114050	51.515	99	35	4	14	111	30	116	3.52e-22	84.7
MS.gene51560.t1	MTR_4g063300	62.687	67	24	1	34	99	17	83	1.16e-19	77.8
MS.gene51560.t1	MTR_6g005150	42.000	100	47	4	14	111	35	125	1.23e-14	65.5
MS.gene51560.t1	MTR_7g427690	78.947	38	8	0	75	112	412	449	2.35e-12	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51560.t1	AT1G52740	91.000	100	9	0	14	113	35	134	9.44e-63	187
MS.gene51560.t1	AT3G54560	91.000	100	9	0	14	113	37	136	6.86e-62	185
MS.gene51560.t1	AT3G54560	91.000	100	9	0	14	113	37	136	6.86e-62	185
MS.gene51560.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene51560.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene51560.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene51560.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene51560.t1	AT4G13570	72.165	97	27	0	7	103	22	118	1.72e-44	140
MS.gene51560.t1	AT4G13570	76.667	90	21	0	14	103	35	124	5.52e-44	139
MS.gene51560.t1	AT1G08880	62.105	95	34	2	14	107	31	124	2.51e-33	113
MS.gene51560.t1	AT1G54690	61.053	95	35	2	14	107	31	124	7.88e-33	112
MS.gene51560.t1	AT4G27230	60.606	99	37	2	14	111	25	122	1.25e-32	111
MS.gene51560.t1	AT4G27230	60.606	99	37	2	14	111	25	122	1.25e-32	111
MS.gene51560.t1	AT5G54640	60.606	99	37	2	14	111	25	122	1.38e-32	111
MS.gene51560.t1	AT1G51060	60.606	99	37	2	14	111	25	122	1.88e-32	110
MS.gene51560.t1	AT3G20670	60.606	99	37	2	14	111	25	122	3.15e-32	110
MS.gene51560.t1	AT5G02560	59.406	101	39	2	14	113	33	132	4.86e-31	107
MS.gene51560.t1	AT5G59870	60.606	99	37	2	14	111	33	130	5.33e-31	107
MS.gene51560.t1	AT5G27670	60.606	99	37	2	14	111	34	131	6.10e-30	105
MS.gene51560.t1	AT5G02560	48.000	125	39	3	14	113	33	156	1.10e-25	95.1

Find 32 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTCTGCAGTTAAATATTCC+AGG	0.413090	1.4:-8765570	None:intergenic
CAATGGCACATGGAAGAGTT+GGG	0.442531	1.4:+8765518	MS.gene51560:CDS
TATGAACATTCATTCCTTCG+AGG	0.453552	1.4:-8765761	None:intergenic
AGTTTATTCTGCCGCTATCC+TGG	0.454001	1.4:+8765552	MS.gene51560:CDS
ACAATGGCACATGGAAGAGT+TGG	0.476378	1.4:+8765517	MS.gene51560:CDS
TGCTTCAGCCACATGTGTCA+TGG	0.477968	1.4:+8765199	None:intergenic
ATATTTAACTGCAGAGGTGT+TGG	0.485770	1.4:+8765576	MS.gene51560:CDS
CTCATCAAAGGAACAATAGC+TGG	0.487626	1.4:+8765694	MS.gene51560:CDS
ATAAGAGACTTGTGGATATG+AGG	0.507095	1.4:-8765730	None:intergenic
AGACGATGGATACGTCCGAC+AGG	0.539450	1.4:-8765481	None:intergenic
AACTGCAGAGGTGTTGGAGT+TGG	0.540859	1.4:+8765582	MS.gene51560:CDS
GCAGAGGTGTTGGAGTTGGC+TGG	0.558822	1.4:+8765586	MS.gene51560:CDS
CAATGCAAGCAAGGATCTGA+AGG	0.565081	1.4:+8765609	MS.gene51560:CDS
GTTGGCTGGCAATGCAAGCA+AGG	0.571379	1.4:+8765600	MS.gene51560:CDS
GAACTTGACACTCTCATCAA+AGG	0.571379	1.4:+8765682	MS.gene51560:CDS
ACTTGTTGATAAGAGACTTG+TGG	0.572936	1.4:-8765738	None:intergenic
ACATGTGTCATGGCTACAGC+AGG	0.574358	1.4:+8765209	None:intergenic
TGTCAAGTTCTTCGTCTCCA+CGG	0.583078	1.4:-8765669	None:intergenic
ATAGCAAGCTGTAGATGTCT+CGG	0.584334	1.4:-8765646	None:intergenic
CATCTACAGCTTGCTATCCG+TGG	0.586408	1.4:+8765652	MS.gene51560:CDS
TCCATCGTCTTCTGAAGTCA+AGG	0.589423	1.4:+8765494	MS.gene51560:CDS
AAAGGAACAATAGCTGGTGG+TGG	0.608922	1.4:+8765700	MS.gene51560:CDS
CCTGGAATATTTAACTGCAG+AGG	0.615265	1.4:+8765570	MS.gene51560:CDS
TCTTATCAACAAGTCCTCGA+AGG	0.615894	1.4:+8765747	MS.gene51560:CDS
AAGTCAAGGACAATGGCACA+TGG	0.622887	1.4:+8765508	MS.gene51560:CDS
GACGATGGATACGTCCGACA+GGG	0.626692	1.4:-8765480	None:intergenic
TTCTACATACAGTTCCCTGT+CGG	0.628096	1.4:+8765466	MS.gene51560:intron
AATGGCACATGGAAGAGTTG+GGG	0.645846	1.4:+8765519	MS.gene51560:CDS
TCTTCTGAAGTCAAGGACAA+TGG	0.655563	1.4:+8765501	MS.gene51560:CDS
ATCAAAGGAACAATAGCTGG+TGG	0.661257	1.4:+8765697	MS.gene51560:CDS
TCCTTGACTTCAGAAGACGA+TGG	0.668973	1.4:-8765495	None:intergenic
AGTTAAATATTCCAGGATAG+CGG	0.686777	1.4:-8765563	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTAAATTATGTTGCATTTGT+TGG	+	chr1.4:8765391-8765410	MS.gene51560:intron	25.0%
!!!	ATTTTAGCATGCTTAAAATG+TGG	-	chr1.4:8765358-8765377	None:intergenic	25.0%
	AATAATTAACCGCTTGCAAA+AGG	-	chr1.4:8765300-8765319	None:intergenic	30.0%
	AGTTAAATATTCCAGGATAG+CGG	-	chr1.4:8765566-8765585	None:intergenic	30.0%
	ATGTAGAAAGATGTTCATGA+TGG	-	chr1.4:8765454-8765473	None:intergenic	30.0%
!	TGATTATTTCCTTTTGCAAG+CGG	+	chr1.4:8765288-8765307	MS.gene51560:intron	30.0%
	ATAAGAGACTTGTGGATATG+AGG	-	chr1.4:8765733-8765752	None:intergenic	35.0%
	ATTTATCCCACATCATCATC+CGG	+	chr1.4:8765424-8765443	MS.gene51560:intron	35.0%
!	ACTTGTTGATAAGAGACTTG+TGG	-	chr1.4:8765741-8765760	None:intergenic	35.0%
!	AGATGTTCATGATGGAAAAC+CGG	-	chr1.4:8765446-8765465	None:intergenic	35.0%
!	ATATTTAACTGCAGAGGTGT+TGG	+	chr1.4:8765576-8765595	MS.gene51560:CDS	35.0%
!!	GCATGCTAAAATTTGTTGTC+TGG	+	chr1.4:8765366-8765385	MS.gene51560:intron	35.0%
	ATAGCAAGCTGTAGATGTCT+CGG	-	chr1.4:8765649-8765668	None:intergenic	40.0%
	ATCAAAGGAACAATAGCTGG+TGG	+	chr1.4:8765697-8765716	MS.gene51560:CDS	40.0%
	CCTCTGCAGTTAAATATTCC+AGG	-	chr1.4:8765573-8765592	None:intergenic	40.0%
	CCTGGAATATTTAACTGCAG+AGG	+	chr1.4:8765570-8765589	MS.gene51560:CDS	40.0%
	CTCATCAAAGGAACAATAGC+TGG	+	chr1.4:8765694-8765713	MS.gene51560:CDS	40.0%
	CTGTGAGTGACTAGTATCTT+TGG	+	chr1.4:8765254-8765273	MS.gene51560:intron	40.0%
	GAACTTGACACTCTCATCAA+AGG	+	chr1.4:8765682-8765701	MS.gene51560:CDS	40.0%
	TCTTATCAACAAGTCCTCGA+AGG	+	chr1.4:8765747-8765766	MS.gene51560:CDS	40.0%
	TTCTACATACAGTTCCCTGT+CGG	+	chr1.4:8765466-8765485	MS.gene51560:intron	40.0%
!!	TCTTCTGAAGTCAAGGACAA+TGG	+	chr1.4:8765501-8765520	MS.gene51560:CDS	40.0%
	AAAGGAACAATAGCTGGTGG+TGG	+	chr1.4:8765700-8765719	MS.gene51560:CDS	45.0%
	AAGTCAAGGACAATGGCACA+TGG	+	chr1.4:8765508-8765527	MS.gene51560:CDS	45.0%
	AATGGCACATGGAAGAGTTG+GGG	+	chr1.4:8765519-8765538	MS.gene51560:CDS	45.0%
	ACAATGGCACATGGAAGAGT+TGG	+	chr1.4:8765517-8765536	MS.gene51560:CDS	45.0%
	AGTTTATTCTGCCGCTATCC+TGG	+	chr1.4:8765552-8765571	MS.gene51560:CDS	45.0%
	CAATGCAAGCAAGGATCTGA+AGG	+	chr1.4:8765609-8765628	MS.gene51560:CDS	45.0%
	CAATGGCACATGGAAGAGTT+GGG	+	chr1.4:8765518-8765537	MS.gene51560:CDS	45.0%
	TCCTTGACTTCAGAAGACGA+TGG	-	chr1.4:8765498-8765517	None:intergenic	45.0%
	TGTCAAGTTCTTCGTCTCCA+CGG	-	chr1.4:8765672-8765691	None:intergenic	45.0%
!	GGAAAACCGGATGATGATGT+GGG	-	chr1.4:8765433-8765452	None:intergenic	45.0%
!	TGGAAAACCGGATGATGATG+TGG	-	chr1.4:8765434-8765453	None:intergenic	45.0%
!!	TCCATCGTCTTCTGAAGTCA+AGG	+	chr1.4:8765494-8765513	MS.gene51560:CDS	45.0%
!!	AACTGCAGAGGTGTTGGAGT+TGG	+	chr1.4:8765582-8765601	MS.gene51560:CDS	50.0%
!!	CATCTACAGCTTGCTATCCG+TGG	+	chr1.4:8765652-8765671	MS.gene51560:CDS	50.0%
	AGACGATGGATACGTCCGAC+AGG	-	chr1.4:8765484-8765503	None:intergenic	55.0%
	GACGATGGATACGTCCGACA+GGG	-	chr1.4:8765483-8765502	None:intergenic	55.0%
	GTTGGCTGGCAATGCAAGCA+AGG	+	chr1.4:8765600-8765619	MS.gene51560:CDS	55.0%
!!	GCAGAGGTGTTGGAGTTGGC+TGG	+	chr1.4:8765586-8765605	MS.gene51560:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	8765211	8765774	8765211	ID=MS.gene51560
chr1.4	mRNA	8765211	8765774	8765211	ID=MS.gene51560.t1;Parent=MS.gene51560
chr1.4	exon	8765211	8765255	8765211	ID=MS.gene51560.t1.exon1;Parent=MS.gene51560.t1
chr1.4	CDS	8765211	8765255	8765211	ID=cds.MS.gene51560.t1;Parent=MS.gene51560.t1
chr1.4	exon	8765478	8765774	8765478	ID=MS.gene51560.t1.exon2;Parent=MS.gene51560.t1
chr1.4	CDS	8765478	8765774	8765478	ID=cds.MS.gene51560.t1;Parent=MS.gene51560.t1

Gene Sequence

>MS.gene51560

ATGTGTCATGGCTACAGCAGGTTCATCTATAACAATGATATTGCTGTGAGTGACTAGTATCTTTGGATGCTGTGTGTTGATTATTTCCTTTTGCAAGCGGTTAATTATTGTATCTCTTTGATTCTTGAAACTATTTTTTTTCTTCCACATTTTAAGCATGCTAAAATTTGTTGTCTGGCTGTAAATTATGTTGCATTTGTTGGTATTGTTTAAATTTATCCCACATCATCATCCGGTTTTCCATCATGAACATCTTTCTACATACAGTTCCCTGTCGGACGTATCCATCGTCTTCTGAAGTCAAGGACAATGGCACATGGAAGAGTTGGGGCTACAGCTGCAGTTTATTCTGCCGCTATCCTGGAATATTTAACTGCAGAGGTGTTGGAGTTGGCTGGCAATGCAAGCAAGGATCTGAAGGTGAAGCGTATTACACCGAGACATCTACAGCTTGCTATCCGTGGAGACGAAGAACTTGACACTCTCATCAAAGGAACAATAGCTGGTGGTGGTGTTATTCCTCATATCCACAAGTCTCTTATCAACAAGTCCTCGAAGGAATGA

Protein sequence

>MS.gene51560.t1

MCHGYSRFIYNNDIAFPVGRIHRLLKSRTMAHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPHIHKSLINKSSKE