Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52120.t1 | XP_003607208.1 | 100 | 50 | 0 | 0 | 1 | 50 | 1 | 50 | 1.00E-17 | 98.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52120.t1 | B7FNA9 | 100.0 | 50 | 0 | 0 | 1 | 50 | 1 | 50 | 2.5e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52120.t1 | A0A072URE6 | 100.0 | 50 | 0 | 0 | 1 | 50 | 1 | 50 | 7.6e-18 | 98.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene52120 | 0.836646 | 8.28E-57 | -1.69E-46 |
| MS.gene052533 | MS.gene52120 | 0.810228 | 1.28E-50 | -1.69E-46 |
| MS.gene053228 | MS.gene52120 | 0.815072 | 1.11E-51 | -1.69E-46 |
| MS.gene053427 | MS.gene52120 | 0.819259 | 1.28E-52 | -1.69E-46 |
| MS.gene054004 | MS.gene52120 | 0.850083 | 2.14E-60 | -1.69E-46 |
| MS.gene054413 | MS.gene52120 | 0.814233 | 1.71E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene52120 | 0.807342 | 5.30E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene52120 | 0.813883 | 2.04E-51 | -1.69E-46 |
| MS.gene059436 | MS.gene52120 | 0.812861 | 3.43E-51 | -1.69E-46 |
| MS.gene059802 | MS.gene52120 | 0.814062 | 1.87E-51 | -1.69E-46 |
| MS.gene060933 | MS.gene52120 | 0.803371 | 3.60E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene52120 | 0.80884 | 2.54E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52120.t1 | MTR_4g074480 | 100.000 | 50 | 0 | 0 | 1 | 50 | 1 | 50 | 1.06e-28 | 102 |
| MS.gene52120.t1 | MTR_4g108000 | 100.000 | 50 | 0 | 0 | 1 | 50 | 1 | 50 | 1.82e-27 | 99.4 |
| MS.gene52120.t1 | MTR_4g108000 | 100.000 | 50 | 0 | 0 | 1 | 50 | 1 | 50 | 3.67e-27 | 100 |
| MS.gene52120.t1 | MTR_4g108000 | 100.000 | 49 | 0 | 0 | 2 | 50 | 17 | 65 | 4.17e-27 | 99.0 |
| MS.gene52120.t1 | MTR_4g074480 | 100.000 | 49 | 0 | 0 | 2 | 50 | 17 | 65 | 5.17e-27 | 98.6 |
| MS.gene52120.t1 | MTR_4g108000 | 100.000 | 49 | 0 | 0 | 2 | 50 | 17 | 65 | 6.84e-27 | 100 |
| MS.gene52120.t1 | MTR_4g108000 | 100.000 | 49 | 0 | 0 | 2 | 50 | 32 | 80 | 1.03e-26 | 98.2 |
| MS.gene52120.t1 | MTR_4g108000 | 100.000 | 49 | 0 | 0 | 2 | 50 | 17 | 65 | 1.09e-26 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGAATTAGTGTATCCTTT+CGG | 0.449557 | 4.1:+29505818 | None:intergenic |
| GGTTGAGAAATTGGATCCTC+TGG | 0.453715 | 4.1:-29505880 | MS.gene52120:CDS |
| TTTGATGCGATTAGAGAGCT+TGG | 0.471851 | 4.1:-29505912 | MS.gene52120:CDS |
| GCATCAAACACTTGACTGAC+AGG | 0.506089 | 4.1:+29505927 | None:intergenic |
| TTGATGCGATTAGAGAGCTT+GGG | 0.506545 | 4.1:-29505911 | MS.gene52120:CDS |
| TGGTGCTGTGTTGGCATGTA+CGG | 0.526353 | 4.1:-29505964 | MS.gene52120:CDS |
| ATTAGAGAGCTTGGGAATGA+GGG | 0.528876 | 4.1:-29505903 | MS.gene52120:CDS |
| GATTAGAGAGCTTGGGAATG+AGG | 0.531987 | 4.1:-29505904 | MS.gene52120:CDS |
| CAGGATGCTGCAAAGATGTA+TGG | 0.549895 | 4.1:-29505984 | MS.gene52120:CDS |
| GACTGAACCTCATCGCCGAA+AGG | 0.600933 | 4.1:-29505833 | MS.gene52120:CDS |
| AAAGATGTATGGTGCTGTGT+TGG | 0.610093 | 4.1:-29505973 | MS.gene52120:CDS |
| AGTGTATCCTTTCGGCGATG+AGG | 0.617470 | 4.1:+29505826 | None:intergenic |
| TTAGAGAGCTTGGGAATGAG+GGG | 0.642564 | 4.1:-29505902 | MS.gene52120:CDS |
| TAGAGAGCTTGGGAATGAGG+GGG | 0.658577 | 4.1:-29505901 | MS.gene52120:CDS |
| GATGCAGATGTAATAACCAG+AGG | 0.692682 | 4.1:+29505864 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTGTAATGATTTGATTT+CGG | - | chr4.1:29506014-29506033 | MS.gene52120:intron | 20.0% |
| ! | ATTTGAGCATGAAACATAAA+AGG | + | chr4.1:29505966-29505985 | None:intergenic | 25.0% |
| ! | TTCATGCTCAAATATTTACA+CGG | - | chr4.1:29505973-29505992 | MS.gene52120:CDS | 25.0% |
| ! | TTTGAGCATGAAACATAAAA+GGG | + | chr4.1:29505965-29505984 | None:intergenic | 25.0% |
| AAAAAAACCAAACACTTTGC+AGG | + | chr4.1:29506276-29506295 | None:intergenic | 30.0% | |
| ATTTGTGGATATGAGATTGA+AGG | - | chr4.1:29506168-29506187 | MS.gene52120:intron | 30.0% | |
| CAATCGTTATTTATTGTCAC+AGG | - | chr4.1:29506356-29506375 | MS.gene52120:intron | 30.0% | |
| TCATGCTCAAATATTTACAC+GGG | - | chr4.1:29505974-29505993 | MS.gene52120:CDS | 30.0% | |
| TGTTTGAAGATGTTAACGAA+AGG | + | chr4.1:29505857-29505876 | None:intergenic | 30.0% | |
| TTTGTGGATATGAGATTGAA+GGG | - | chr4.1:29506169-29506188 | MS.gene52120:intron | 30.0% | |
| TTTGTTAAAAACGATGTTCC+TGG | - | chr4.1:29506132-29506151 | MS.gene52120:intron | 30.0% | |
| !! | ATGATTTGATTTCGGCTTTT+TGG | - | chr4.1:29506022-29506041 | MS.gene52120:intron | 30.0% |
| !! | TTGTGATTGATGGTTTTCTA+TGG | - | chr4.1:29506194-29506213 | MS.gene52120:intron | 30.0% |
| AGGATTTGAAAGAGATAGAG+AGG | + | chr4.1:29505837-29505856 | None:intergenic | 35.0% | |
| CTTGGACTGCTTTGATTAAT+GGG | - | chr4.1:29506109-29506128 | MS.gene52120:intron | 35.0% | |
| GTTTGATGAAAGTCCTCAAA+GGG | - | chr4.1:29506077-29506096 | MS.gene52120:intron | 35.0% | |
| TGTTTGATGAAAGTCCTCAA+AGG | - | chr4.1:29506076-29506095 | MS.gene52120:intron | 35.0% | |
| TTCTGTTTATTGTGCTCTTG+TGG | - | chr4.1:29506217-29506236 | MS.gene52120:intron | 35.0% | |
| TTTACGATAACCTGTTCCAT+TGG | - | chr4.1:29505933-29505952 | MS.gene52120:CDS | 35.0% | |
| ATAACCTGTTCCATTGGTGA+TGG | - | chr4.1:29505939-29505958 | MS.gene52120:CDS | 40.0% | |
| ATTAGAGAGCTTGGGAATGA+GGG | - | chr4.1:29506456-29506475 | MS.gene52120:intron | 40.0% | |
| CAAGCAACAATATCCCTTTG+AGG | + | chr4.1:29506093-29506112 | None:intergenic | 40.0% | |
| CTCAAAGGGATATTGTTGCT+TGG | - | chr4.1:29506091-29506110 | MS.gene52120:intron | 40.0% | |
| GATGCAGATGTAATAACCAG+AGG | + | chr4.1:29506498-29506517 | None:intergenic | 40.0% | |
| GCTTGGACTGCTTTGATTAA+TGG | - | chr4.1:29506108-29506127 | MS.gene52120:intron | 40.0% | |
| TAAAAACGATGTTCCTGGTG+AGG | - | chr4.1:29506137-29506156 | MS.gene52120:intron | 40.0% | |
| TTGATGCGATTAGAGAGCTT+GGG | - | chr4.1:29506448-29506467 | MS.gene52120:intron | 40.0% | |
| TTTGATGCGATTAGAGAGCT+TGG | - | chr4.1:29506447-29506466 | MS.gene52120:intron | 40.0% | |
| ! | GTGGACATGTATTTCAAGTG+TGG | - | chr4.1:29506236-29506255 | MS.gene52120:intron | 40.0% |
| !! | AAAGATGTATGGTGCTGTGT+TGG | - | chr4.1:29506386-29506405 | MS.gene52120:intron | 40.0% |
| !! | TTGAAGGGTGTTGTGATTGA+TGG | - | chr4.1:29506184-29506203 | MS.gene52120:intron | 40.0% |
| !!! | ATTTCGGCTTTTTGGTGTTC+TGG | - | chr4.1:29506030-29506049 | MS.gene52120:intron | 40.0% |
| !!! | TTTCGGCTTTTTGGTGTTCT+GGG | - | chr4.1:29506031-29506050 | MS.gene52120:intron | 40.0% |
| CAGGATGCTGCAAAGATGTA+TGG | - | chr4.1:29506375-29506394 | MS.gene52120:intron | 45.0% | |
| CATAAAAGGGCCATCACCAA+TGG | + | chr4.1:29505952-29505971 | None:intergenic | 45.0% | |
| GAATGAGGGGGTTGAGAAAT+TGG | - | chr4.1:29506470-29506489 | MS.gene52120:intron | 45.0% | |
| GATTAGAGAGCTTGGGAATG+AGG | - | chr4.1:29506455-29506474 | MS.gene52120:intron | 45.0% | |
| GCATCAAACACTTGACTGAC+AGG | + | chr4.1:29506435-29506454 | None:intergenic | 45.0% | |
| GGTTGAGAAATTGGATCCTC+TGG | - | chr4.1:29506479-29506498 | MS.gene52120:intron | 45.0% | |
| TTAGAGAGCTTGGGAATGAG+GGG | - | chr4.1:29506457-29506476 | MS.gene52120:intron | 45.0% | |
| ! | GATGATGCCTGCAAAGTGTT+TGG | - | chr4.1:29506266-29506285 | MS.gene52120:intron | 45.0% |
| AGTGTATCCTTTCGGCGATG+AGG | + | chr4.1:29506536-29506555 | None:intergenic | 50.0% | |
| CAAATCACTTGTGCCTCACC+AGG | + | chr4.1:29506153-29506172 | None:intergenic | 50.0% | |
| GGTGAGGCACAAGTGATTTG+TGG | - | chr4.1:29506153-29506172 | MS.gene52120:intron | 50.0% | |
| TAGAGAGCTTGGGAATGAGG+GGG | - | chr4.1:29506458-29506477 | MS.gene52120:intron | 50.0% | |
| !! | TGGTGCTGTGTTGGCATGTA+CGG | - | chr4.1:29506395-29506414 | MS.gene52120:intron | 50.0% |
| AGGGCCATCACCAATGGAAC+AGG | + | chr4.1:29505946-29505965 | None:intergenic | 55.0% | |
| GACTGAACCTCATCGCCGAA+AGG | - | chr4.1:29506526-29506545 | MS.gene52120:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 29505824 | 29506557 | 29505824 | ID=MS.gene52120 |
| chr4.1 | mRNA | 29505824 | 29506557 | 29505824 | ID=MS.gene52120.t1;Parent=MS.gene52120 |
| chr4.1 | exon | 29506555 | 29506557 | 29506555 | ID=MS.gene52120.t1.exon1;Parent=MS.gene52120.t1 |
| chr4.1 | CDS | 29506555 | 29506557 | 29506555 | ID=cds.MS.gene52120.t1;Parent=MS.gene52120.t1 |
| chr4.1 | exon | 29505824 | 29506003 | 29505824 | ID=MS.gene52120.t1.exon2;Parent=MS.gene52120.t1 |
| chr4.1 | CDS | 29505824 | 29506003 | 29505824 | ID=cds.MS.gene52120.t1;Parent=MS.gene52120.t1 |
| Gene Sequence |
| Protein sequence |
