Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52248.t1 | RHN46941.1 | 81.8 | 77 | 13 | 1 | 1 | 76 | 39 | 115 | 1.30E-24 | 122.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52248.t1 | Q41066 | 67.1 | 73 | 18 | 2 | 4 | 76 | 3 | 69 | 3.6e-18 | 92.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52248.t1 | G7L4L7 | 81.8 | 77 | 13 | 1 | 1 | 76 | 1 | 77 | 9.6e-25 | 122.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene52248 | 0.821047 | 4.97E-53 | -1.69E-46 |
| MS.gene050426 | MS.gene52248 | 0.81125 | 7.69E-51 | -1.69E-46 |
| MS.gene050971 | MS.gene52248 | -0.804476 | 2.12E-49 | -1.69E-46 |
| MS.gene052223 | MS.gene52248 | 0.821346 | 4.24E-53 | -1.69E-46 |
| MS.gene05233 | MS.gene52248 | 0.806396 | 8.39E-50 | -1.69E-46 |
| MS.gene052702 | MS.gene52248 | 0.815633 | 8.36E-52 | -1.69E-46 |
| MS.gene054349 | MS.gene52248 | -0.806439 | 8.22E-50 | -1.69E-46 |
| MS.gene054742 | MS.gene52248 | 0.801507 | 8.71E-49 | -1.69E-46 |
| MS.gene055601 | MS.gene52248 | 0.820343 | 7.21E-53 | -1.69E-46 |
| MS.gene056330 | MS.gene52248 | 0.815651 | 8.29E-52 | -1.69E-46 |
| MS.gene056690 | MS.gene52248 | 0.810167 | 1.32E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene52248 | 0.816317 | 5.89E-52 | -1.69E-46 |
| MS.gene056920 | MS.gene52248 | 0.802168 | 6.38E-49 | -1.69E-46 |
| MS.gene059402 | MS.gene52248 | 0.827286 | 1.70E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52248.t1 | MTR_7g077340 | 86.667 | 120 | 14 | 2 | 1 | 118 | 1 | 120 | 1.47e-71 | 209 |
| MS.gene52248.t1 | MTR_7g077215 | 81.818 | 99 | 16 | 1 | 13 | 111 | 14 | 110 | 6.34e-53 | 162 |
| MS.gene52248.t1 | MTR_7g077210 | 61.667 | 120 | 33 | 6 | 1 | 111 | 1 | 116 | 2.13e-40 | 130 |
| MS.gene52248.t1 | MTR_7g077180 | 63.063 | 111 | 16 | 2 | 1 | 111 | 1 | 86 | 7.95e-39 | 125 |
| MS.gene52248.t1 | MTR_7g077310 | 59.615 | 104 | 38 | 2 | 11 | 111 | 1 | 103 | 5.31e-37 | 121 |
| MS.gene52248.t1 | MTR_7g077260 | 54.386 | 114 | 48 | 3 | 1 | 111 | 1 | 113 | 4.92e-35 | 117 |
| MS.gene52248.t1 | MTR_7g077220 | 61.458 | 96 | 25 | 1 | 10 | 105 | 1 | 84 | 2.81e-34 | 115 |
| MS.gene52248.t1 | MTR_7g077300 | 55.462 | 119 | 42 | 4 | 1 | 118 | 1 | 109 | 4.99e-34 | 114 |
| MS.gene52248.t1 | MTR_7g077160 | 55.556 | 108 | 43 | 1 | 11 | 118 | 5 | 107 | 3.23e-30 | 104 |
| MS.gene52248.t1 | MTR_7g077313 | 49.533 | 107 | 45 | 4 | 4 | 108 | 3 | 102 | 4.55e-23 | 86.3 |
| MS.gene52248.t1 | MTR_7g077350 | 50.877 | 114 | 46 | 5 | 6 | 112 | 23 | 133 | 5.04e-22 | 84.7 |
| MS.gene52248.t1 | MTR_7g077190 | 55.000 | 60 | 27 | 0 | 54 | 113 | 22 | 81 | 1.21e-16 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGTCACTGTGCTGATAT+TGG | 0.334596 | 7.4:+28657920 | MS.gene52248:CDS |
| AATGATGAAGTTAGCTTTGT+TGG | 0.339085 | 7.4:+28657739 | MS.gene52248:CDS |
| TTTGAGCTTCACTGCAACCA+TGG | 0.462992 | 7.4:+28657769 | MS.gene52248:CDS |
| TGGTTGCAGTGAAGCTCAAA+AGG | 0.467432 | 7.4:-28657766 | None:intergenic |
| AACTTAGTGAGATCCACCTT+TGG | 0.468934 | 7.4:-28658050 | None:intergenic |
| CCACCTTTGGAGTAGCTACA+TGG | 0.481606 | 7.4:-28658037 | None:intergenic |
| GGAGAGGAGTTGAGTGATGA+AGG | 0.494529 | 7.4:-28657816 | None:intergenic |
| ATCACAGCATGCTGTTGTTG+TGG | 0.550411 | 7.4:-28657867 | None:intergenic |
| ATCGTAACTAGCTTCACCAT+TGG | 0.556263 | 7.4:-28657837 | None:intergenic |
| ATCACTCAACTCCTCTCCAA+TGG | 0.592346 | 7.4:+28657821 | MS.gene52248:CDS |
| CCATGTAGCTACTCCAAAGG+TGG | 0.604280 | 7.4:+28658037 | MS.gene52248:CDS |
| CAGCACAGTGACACTGAGGA+GGG | 0.627236 | 7.4:-28657913 | None:intergenic |
| GAGGAAAAGATCTTGTGCAA+AGG | 0.628614 | 7.4:-28657973 | None:intergenic |
| TCAGCACAGTGACACTGAGG+AGG | 0.630876 | 7.4:-28657914 | None:intergenic |
| AACTAGCTTCACCATTGGAG+AGG | 0.633390 | 7.4:-28657832 | None:intergenic |
| GAACCATGTAGCTACTCCAA+AGG | 0.655168 | 7.4:+28658034 | MS.gene52248:CDS |
| ATCGAAACGAGCATCAACCA+TGG | 0.682629 | 7.4:-28657786 | None:intergenic |
| TCAGTGCAACGACACTGTGG+AGG | 0.684993 | 7.4:-28657992 | None:intergenic |
| TGGTTCATAGCAGAAGTCAG+TGG | 0.693755 | 7.4:-28658017 | None:intergenic |
| ATATCAGCACAGTGACACTG+AGG | 0.763170 | 7.4:-28657917 | None:intergenic |
| GTATCAGTGCAACGACACTG+TGG | 0.776006 | 7.4:-28657995 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGATGAAGTTAGCTTTGT+TGG | + | chr7.4:28657739-28657758 | MS.gene52248:CDS | 30.0% |
| GAGGAAAAGATCTTGTGCAA+AGG | - | chr7.4:28657976-28657995 | None:intergenic | 40.0% | |
| ! | ATCGTAACTAGCTTCACCAT+TGG | - | chr7.4:28657840-28657859 | None:intergenic | 40.0% |
| !! | GAGGGATTGATTTTGTGCAA+AGG | - | chr7.4:28657898-28657917 | None:intergenic | 40.0% |
| ATATCAGCACAGTGACACTG+AGG | - | chr7.4:28657920-28657939 | None:intergenic | 45.0% | |
| ATCACAGCATGCTGTTGTTG+TGG | - | chr7.4:28657870-28657889 | None:intergenic | 45.0% | |
| ATCACTCAACTCCTCTCCAA+TGG | + | chr7.4:28657821-28657840 | MS.gene52248:CDS | 45.0% | |
| ATCGAAACGAGCATCAACCA+TGG | - | chr7.4:28657789-28657808 | None:intergenic | 45.0% | |
| GAACCATGTAGCTACTCCAA+AGG | + | chr7.4:28658034-28658053 | MS.gene52248:CDS | 45.0% | |
| TGGTTCATAGCAGAAGTCAG+TGG | - | chr7.4:28658020-28658039 | None:intergenic | 45.0% | |
| TGGTTGCAGTGAAGCTCAAA+AGG | - | chr7.4:28657769-28657788 | None:intergenic | 45.0% | |
| TTTGAGCTTCACTGCAACCA+TGG | + | chr7.4:28657769-28657788 | MS.gene52248:CDS | 45.0% | |
| ! | AACTAGCTTCACCATTGGAG+AGG | - | chr7.4:28657835-28657854 | None:intergenic | 45.0% |
| ! | CAGTGTCACTGTGCTGATAT+TGG | + | chr7.4:28657920-28657939 | MS.gene52248:CDS | 45.0% |
| CCATGTAGCTACTCCAAAGG+TGG | + | chr7.4:28658037-28658056 | MS.gene52248:CDS | 50.0% | |
| GGAGAGGAGTTGAGTGATGA+AGG | - | chr7.4:28657819-28657838 | None:intergenic | 50.0% | |
| GTATCAGTGCAACGACACTG+TGG | - | chr7.4:28657998-28658017 | None:intergenic | 50.0% | |
| !! | CCACCTTTGGAGTAGCTACA+TGG | - | chr7.4:28658040-28658059 | None:intergenic | 50.0% |
| CAGCACAGTGACACTGAGGA+GGG | - | chr7.4:28657916-28657935 | None:intergenic | 55.0% | |
| TCAGCACAGTGACACTGAGG+AGG | - | chr7.4:28657917-28657936 | None:intergenic | 55.0% | |
| TCAGTGCAACGACACTGTGG+AGG | - | chr7.4:28657995-28658014 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 28657713 | 28658069 | 28657713 | ID=MS.gene52248 |
| chr7.4 | mRNA | 28657713 | 28658069 | 28657713 | ID=MS.gene52248.t1;Parent=MS.gene52248 |
| chr7.4 | exon | 28657713 | 28658069 | 28657713 | ID=MS.gene52248.t1.exon1;Parent=MS.gene52248.t1 |
| chr7.4 | CDS | 28657713 | 28658069 | 28657713 | ID=cds.MS.gene52248.t1;Parent=MS.gene52248.t1 |
| Gene Sequence |
| Protein sequence |
