AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05233


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05233.t1 MTR_6g078280 94.836 213 9 1 1 213 1 211 1.23e-141 394
MS.gene05233.t1 MTR_6g078260 67.742 217 47 7 1 213 1 198 1.59e-93 272
MS.gene05233.t1 MTR_6g078140 58.911 202 75 4 15 213 16 212 5.20e-73 220
MS.gene05233.t1 MTR_6g078120 58.416 202 76 4 15 213 16 212 3.08e-72 218
MS.gene05233.t1 MTR_6g078290 51.852 216 94 5 1 212 1 210 3.99e-67 205
MS.gene05233.t1 MTR_6g078250 52.074 217 88 6 1 214 1 204 1.84e-62 193
MS.gene05233.t1 MTR_7g037410 43.979 191 90 6 26 213 25 201 1.59e-48 158
MS.gene05233.t1 MTR_6g078100 37.788 217 118 8 1 213 1 204 7.69e-35 123
MS.gene05233.t1 MTR_6g078070 35.747 221 115 10 1 213 1 202 1.96e-30 111
MS.gene05233.t1 MTR_6g065460 29.767 215 133 7 1 211 1 201 8.28e-16 73.2
MS.gene05233.t1 MTR_6g009563 29.756 205 119 7 6 205 9 193 2.34e-12 63.9
MS.gene05233.t1 MTR_3g106620 29.515 227 114 13 6 214 1 199 1.15e-11 62.0
MS.gene05233.t1 MTR_6g009650 29.952 207 118 7 4 205 8 192 1.29e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05233.t1 AT1G17860 36.239 218 110 9 1 213 1 194 1.41e-36 127
MS.gene05233.t1 AT1G73325 33.480 227 112 12 1 213 1 202 9.20e-22 89.4
MS.gene05233.t1 AT1G73260 30.047 213 124 10 8 214 12 205 6.73e-18 79.0
MS.gene05233.t1 AT1G73330 29.596 223 117 13 1 214 1 192 2.54e-13 66.6

Find 57 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTGAAACTTAACTTTAA+AGG 0.154767 6.2:-77792066 None:intergenic
CATCTTCATACTTCTCAATT+TGG 0.163418 6.2:-77791923 None:intergenic
AATGTTTCTTCTTTAGAAAA+AGG 0.285974 6.2:-77791843 None:intergenic
TTCTGAAACTTAACTTTAAA+GGG 0.298093 6.2:-77792065 None:intergenic
AGCCGGAATGATGTAGTAAT+TGG 0.358723 6.2:-77791574 None:intergenic
AACCATGTATGCAAAGATAT+TGG 0.371437 6.2:+77791993 MS.gene05233:CDS
AACGCCTTTCTTGTTGTTAA+CGG 0.386453 6.2:-77791730 None:intergenic
TTGTTAGTTGTTTCAATAGT+GGG 0.387109 6.2:+77791619 MS.gene05233:CDS
GTGACTACTAATGGTGTATT+GGG 0.404413 6.2:+77791867 MS.gene05233:CDS
ACGCCTTTCTTGTTGTTAAC+GGG 0.404872 6.2:-77791729 None:intergenic
TTCCAATATCTTTGCATACA+TGG 0.418385 6.2:-77791995 None:intergenic
GAAACATTTGTGACTACTAA+TGG 0.419288 6.2:+77791858 MS.gene05233:CDS
TTTGTTAGTTGTTTCAATAG+TGG 0.428561 6.2:+77791618 MS.gene05233:CDS
TGTGACTACTAATGGTGTAT+TGG 0.442930 6.2:+77791866 MS.gene05233:CDS
TACCAATTACTACATCATTC+CGG 0.457107 6.2:+77791572 MS.gene05233:CDS
TAAGACTAGGGAAATGCGTT+TGG 0.471251 6.2:+77792028 MS.gene05233:CDS
CTTAACTTTAAAGGGAACAT+TGG 0.476364 6.2:-77792057 None:intergenic
GTTCAAATACAATCCACAAT+TGG 0.476445 6.2:+77791898 MS.gene05233:CDS
TCTCAATTTGGAACCAATTG+TGG 0.486271 6.2:-77791911 None:intergenic
ACATGGTTGCAAGTAGGCCT+AGG 0.486695 6.2:-77791978 None:intergenic
TTGTCCTCACAACCACTACT+TGG 0.492527 6.2:+77791489 MS.gene05233:CDS
CGATGTGTCAATGACTTGAT+CGG 0.502159 6.2:-77791529 None:intergenic
GTAGGCCTAGGGCACACATT+AGG 0.504846 6.2:-77791966 None:intergenic
GAACTTTATGTTGAGATCAG+TGG 0.505826 6.2:-77791763 None:intergenic
ATTGTCCTAATGTGTGCCCT+AGG 0.513630 6.2:+77791961 MS.gene05233:CDS
GAATTTATGTGTATAAGACT+AGG 0.515322 6.2:+77792015 MS.gene05233:CDS
TGTTAGTTGTTTCAATAGTG+GGG 0.526839 6.2:+77791620 MS.gene05233:CDS
GTAGTTGTGAAACATAATCA+AGG 0.526996 6.2:+77791687 MS.gene05233:CDS
AATTTATGTGTATAAGACTA+GGG 0.531831 6.2:+77792016 MS.gene05233:CDS
AGGATCAATCTTCCACACTA+AGG 0.534325 6.2:-77791823 None:intergenic
GTTAGTTGTTTCAATAGTGG+GGG 0.535688 6.2:+77791621 MS.gene05233:CDS
TTGTGGATTGTATTTGAACC+CGG 0.536118 6.2:-77791894 None:intergenic
GTATGAAGATGCTTATAAGT+TGG 0.537055 6.2:+77791935 MS.gene05233:CDS
ATAATCAAGGTTTACCGTTG+AGG 0.542322 6.2:+77791700 MS.gene05233:CDS
TTCCACACTAAGGAATGGTT+AGG 0.542536 6.2:-77791813 None:intergenic
GATGTGTCAATGACTTGATC+GGG 0.551157 6.2:-77791528 None:intergenic
TAATGGTGTATTGGGAAACC+CGG 0.551726 6.2:+77791875 MS.gene05233:CDS
CTTTAAAGTTAAGTTTCAGA+AGG 0.553919 6.2:+77792067 MS.gene05233:CDS
TTGCATACATGGTTGCAAGT+AGG 0.561986 6.2:-77791984 None:intergenic
GTCCTAACCATTCCTTAGTG+TGG 0.567409 6.2:+77791811 MS.gene05233:CDS
GGCATTGCTCTTGAATCCGT+CGG 0.567668 6.2:+77791642 MS.gene05233:CDS
ACACCCGTTAACAACAAGAA+AGG 0.576147 6.2:+77791726 MS.gene05233:CDS
GATCAAGTCATTGACACATC+GGG 0.588958 6.2:+77791531 MS.gene05233:CDS
TTCACAACTACTACATCAAG+AGG 0.592646 6.2:-77791675 None:intergenic
CAATCTTCCACACTAAGGAA+TGG 0.596624 6.2:-77791818 None:intergenic
GTGAGGACAATGCAAAGAGA+AGG 0.598876 6.2:-77791476 None:intergenic
TGTGGATTGTATTTGAACCC+GGG 0.605984 6.2:-77791893 None:intergenic
GCTACGTCATTTACAACATG+TGG 0.606180 6.2:+77791594 MS.gene05233:CDS
CATGGTTGCAAGTAGGCCTA+GGG 0.612576 6.2:-77791977 None:intergenic
CATGTTGTAAATGACGTAGC+CGG 0.612691 6.2:-77791591 None:intergenic
CGATCAAGTCATTGACACAT+CGG 0.616565 6.2:+77791530 MS.gene05233:CDS
TTAACGGGTGTGAACCTCAA+CGG 0.621698 6.2:-77791714 None:intergenic
TGATCCAAGTAGTGGTTGTG+AGG 0.632530 6.2:-77791493 None:intergenic
GCTTCAGCTGATCCAAGTAG+TGG 0.646579 6.2:-77791501 None:intergenic
AATGGTGTATTGGGAAACCC+GGG 0.666072 6.2:+77791876 MS.gene05233:CDS
AAGAGGGCAAGATTCGCCGA+CGG 0.698733 6.2:-77791658 None:intergenic
TCACAACTACTACATCAAGA+GGG 0.703659 6.2:-77791674 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATGTGTATAAGACTA+GGG + chr6.2:77792016-77792035 MS.gene05233:CDS 20.0%
!!! AATGTTTCTTCTTTAGAAAA+AGG - chr6.2:77791846-77791865 None:intergenic 20.0%
!!! TTCTGAAACTTAACTTTAAA+GGG - chr6.2:77792068-77792087 None:intergenic 20.0%
! GAATTTATGTGTATAAGACT+AGG + chr6.2:77792015-77792034 MS.gene05233:CDS 25.0%
!! TTGTTAGTTGTTTCAATAGT+GGG + chr6.2:77791619-77791638 MS.gene05233:CDS 25.0%
!! TTTGTTAGTTGTTTCAATAG+TGG + chr6.2:77791618-77791637 MS.gene05233:CDS 25.0%
!!! CTTCTGAAACTTAACTTTAA+AGG - chr6.2:77792069-77792088 None:intergenic 25.0%
!!! CTTTAAAGTTAAGTTTCAGA+AGG + chr6.2:77792067-77792086 MS.gene05233:CDS 25.0%
AACCATGTATGCAAAGATAT+TGG + chr6.2:77791993-77792012 MS.gene05233:CDS 30.0%
CATCTTCATACTTCTCAATT+TGG - chr6.2:77791926-77791945 None:intergenic 30.0%
GAAACATTTGTGACTACTAA+TGG + chr6.2:77791858-77791877 MS.gene05233:CDS 30.0%
GTAGTTGTGAAACATAATCA+AGG + chr6.2:77791687-77791706 MS.gene05233:CDS 30.0%
GTATGAAGATGCTTATAAGT+TGG + chr6.2:77791935-77791954 MS.gene05233:CDS 30.0%
GTTCAAATACAATCCACAAT+TGG + chr6.2:77791898-77791917 MS.gene05233:CDS 30.0%
TACCAATTACTACATCATTC+CGG + chr6.2:77791572-77791591 MS.gene05233:CDS 30.0%
TTCCAATATCTTTGCATACA+TGG - chr6.2:77791998-77792017 None:intergenic 30.0%
! CTTAACTTTAAAGGGAACAT+TGG - chr6.2:77792060-77792079 None:intergenic 30.0%
! TGTTAGTTGTTTCAATAGTG+GGG + chr6.2:77791620-77791639 MS.gene05233:CDS 30.0%
ATAATCAAGGTTTACCGTTG+AGG + chr6.2:77791700-77791719 MS.gene05233:CDS 35.0%
GAACTTTATGTTGAGATCAG+TGG - chr6.2:77791766-77791785 None:intergenic 35.0%
GTGACTACTAATGGTGTATT+GGG + chr6.2:77791867-77791886 MS.gene05233:CDS 35.0%
TCACAACTACTACATCAAGA+GGG - chr6.2:77791677-77791696 None:intergenic 35.0%
TGTGACTACTAATGGTGTAT+TGG + chr6.2:77791866-77791885 MS.gene05233:CDS 35.0%
TTCACAACTACTACATCAAG+AGG - chr6.2:77791678-77791697 None:intergenic 35.0%
! GTTAGTTGTTTCAATAGTGG+GGG + chr6.2:77791621-77791640 MS.gene05233:CDS 35.0%
! TCTCAATTTGGAACCAATTG+TGG - chr6.2:77791914-77791933 None:intergenic 35.0%
! TTGTGGATTGTATTTGAACC+CGG - chr6.2:77791897-77791916 None:intergenic 35.0%
!! AACGCCTTTCTTGTTGTTAA+CGG - chr6.2:77791733-77791752 None:intergenic 35.0%
ACACCCGTTAACAACAAGAA+AGG + chr6.2:77791726-77791745 MS.gene05233:CDS 40.0%
AGCCGGAATGATGTAGTAAT+TGG - chr6.2:77791577-77791596 None:intergenic 40.0%
AGGATCAATCTTCCACACTA+AGG - chr6.2:77791826-77791845 None:intergenic 40.0%
CAATCTTCCACACTAAGGAA+TGG - chr6.2:77791821-77791840 None:intergenic 40.0%
CATGTTGTAAATGACGTAGC+CGG - chr6.2:77791594-77791613 None:intergenic 40.0%
CGATCAAGTCATTGACACAT+CGG + chr6.2:77791530-77791549 MS.gene05233:CDS 40.0%
CGATGTGTCAATGACTTGAT+CGG - chr6.2:77791532-77791551 None:intergenic 40.0%
GATCAAGTCATTGACACATC+GGG + chr6.2:77791531-77791550 MS.gene05233:CDS 40.0%
GATGTGTCAATGACTTGATC+GGG - chr6.2:77791531-77791550 None:intergenic 40.0%
GCTACGTCATTTACAACATG+TGG + chr6.2:77791594-77791613 MS.gene05233:CDS 40.0%
TAAGACTAGGGAAATGCGTT+TGG + chr6.2:77792028-77792047 MS.gene05233:CDS 40.0%
TAATGGTGTATTGGGAAACC+CGG + chr6.2:77791875-77791894 MS.gene05233:CDS 40.0%
TTCCACACTAAGGAATGGTT+AGG - chr6.2:77791816-77791835 None:intergenic 40.0%
TTGCATACATGGTTGCAAGT+AGG - chr6.2:77791987-77792006 None:intergenic 40.0%
! TGTGGATTGTATTTGAACCC+GGG - chr6.2:77791896-77791915 None:intergenic 40.0%
!! ACGCCTTTCTTGTTGTTAAC+GGG - chr6.2:77791732-77791751 None:intergenic 40.0%
AATGGTGTATTGGGAAACCC+GGG + chr6.2:77791876-77791895 MS.gene05233:CDS 45.0%
ATTGTCCTAATGTGTGCCCT+AGG + chr6.2:77791961-77791980 MS.gene05233:CDS 45.0%
GTCCTAACCATTCCTTAGTG+TGG + chr6.2:77791811-77791830 MS.gene05233:CDS 45.0%
GTGAGGACAATGCAAAGAGA+AGG - chr6.2:77791479-77791498 None:intergenic 45.0%
TGATCCAAGTAGTGGTTGTG+AGG - chr6.2:77791496-77791515 None:intergenic 45.0%
TTGTCCTCACAACCACTACT+TGG + chr6.2:77791489-77791508 MS.gene05233:CDS 45.0%
! TTAACGGGTGTGAACCTCAA+CGG - chr6.2:77791717-77791736 None:intergenic 45.0%
ACATGGTTGCAAGTAGGCCT+AGG - chr6.2:77791981-77792000 None:intergenic 50.0%
CATGGTTGCAAGTAGGCCTA+GGG - chr6.2:77791980-77791999 None:intergenic 50.0%
GCTTCAGCTGATCCAAGTAG+TGG - chr6.2:77791504-77791523 None:intergenic 50.0%
GGCATTGCTCTTGAATCCGT+CGG + chr6.2:77791642-77791661 MS.gene05233:CDS 50.0%
AAGAGGGCAAGATTCGCCGA+CGG - chr6.2:77791661-77791680 None:intergenic 55.0%
GTAGGCCTAGGGCACACATT+AGG - chr6.2:77791969-77791988 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 77791450 77792094 77791450 ID=MS.gene05233
chr6.2 mRNA 77791450 77792094 77791450 ID=MS.gene05233.t1;Parent=MS.gene05233
chr6.2 exon 77791450 77792094 77791450 ID=MS.gene05233.t1.exon1;Parent=MS.gene05233.t1
chr6.2 CDS 77791450 77792094 77791450 ID=cds.MS.gene05233.t1;Parent=MS.gene05233.t1
Gene Sequence

>MS.gene05233

ATGAAGAGTACAATACTAGCATTTCTCCTTCTCTTTGCATTGTCCTCACAACCACTACTTGGATCAGCTGAAGCTTCACCCGATCAAGTCATTGACACATCGGGCAAGAAGCTTCGAGCTGATACCAATTACTACATCATTCCGGCTACGTCATTTACAACATGTGGCTTTGTTAGTTGTTTCAATAGTGGGGGCATTGCTCTTGAATCCGTCGGCGAATCTTGCCCTCTTGATGTAGTAGTTGTGAAACATAATCAAGGTTTACCGTTGAGGTTCACACCCGTTAACAACAAGAAAGGCGTTATTCGTGTCTCCACTGATCTCAACATAAAGTTCTCTAATGATGCTTATGATTCTAGATGTCCTAACCATTCCTTAGTGTGGAAGATTGATCCTTTTTCTAAAGAAGAAACATTTGTGACTACTAATGGTGTATTGGGAAACCCGGGTTCAAATACAATCCACAATTGGTTCCAAATTGAGAAGTATGAAGATGCTTATAAGTTGGTCTATTGTCCTAATGTGTGCCCTAGGCCTACTTGCAACCATGTATGCAAAGATATTGGAATTTATGTGTATAAGACTAGGGAAATGCGTTTGGCTCTCACCAATGTTCCCTTTAAAGTTAAGTTTCAGAAGGTTTGA

Protein sequence

>MS.gene05233.t1

MKSTILAFLLLFALSSQPLLGSAEASPDQVIDTSGKKLRADTNYYIIPATSFTTCGFVSCFNSGGIALESVGESCPLDVVVVKHNQGLPLRFTPVNNKKGVIRVSTDLNIKFSNDAYDSRCPNHSLVWKIDPFSKEETFVTTNGVLGNPGSNTIHNWFQIEKYEDAYKLVYCPNVCPRPTCNHVCKDIGIYVYKTREMRLALTNVPFKVKFQKV